CN104221958A - Artificial spawning method for coreius guichenoti - Google Patents
Artificial spawning method for coreius guichenoti Download PDFInfo
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- CN104221958A CN104221958A CN201410408753.6A CN201410408753A CN104221958A CN 104221958 A CN104221958 A CN 104221958A CN 201410408753 A CN201410408753 A CN 201410408753A CN 104221958 A CN104221958 A CN 104221958A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention discloses an artificial spawning method for coreius guichenoti. The artificial spawning method comprises the following steps: selecting parent fish, preparing and injecting a spawning medicine, controlling a spawning tank and water quality, carrying out artificial insemination and incubating. By adopting the artificial spawning method, the mature coreius guichenoti lays eggs and expels sperms under an artificial biological condition through a method of artificially injecting hormone to induce; fertilized ova can be obtained by an artificial insemination method and fish fries are incubated in an incubation equipment, so that the artificial propagation of the coreius guichenoti under a fully-artificial control condition is realized.
Description
Technical field
The invention belongs to technical field of aquaculture, be specifically related to a kind of C. guichenoti induced spawning method.
Background technology
C. guichenoti (Coreius guichenoti) is under the jurisdiction of carp Xing Mu Minnow subfamily copper fish and belongs to, and is Main Commercial Fishes and the endemic species of Upper Yangtze River, is typical river course migration fishes and product Drifting egg fish.In history, C. guichenoti is comparatively large in the population quantity of Yangtze upriver areas, is the main fished species of Upper Yangtze River river section.At present, the spawning ground of C. guichenoti is only found in Jinsha jiang River middle and lower reaches and downstream, Yalongjiang River master stream, along with the progressively enforcement of Jinsha jiang River middle and lower reaches, Yalongjiang River master stream and main stream of the Yangtze hydroelectric development, not only its passage completing the history of life will dam because building a dam and be blocked, and its spawning ground environment likely suffers catastrophic collapse.Meanwhile, due to long-term overfishing, immoderate resource is robbed, and C. guichenoti Stock resoures has presented obvious downward trend, and its species viability faces huge threat with continuity.Day by day increase in Jinsha jiang River and Upper Yangtze River hydroelectric project; when C. guichenoti Natural Population resource and suitable egg reproduction environmental condition reduce day by day, the artificial fecundation of reinforcement C. guichenoti is released and is become the inevitable choice of protection & restoration C. guichenoti Stock resoures.Meanwhile, C. guichenoti fine and tender taste, delicious flavour is the high-quality edible fishes of China Yangtze river basin, and the huge market demand is carried out it and propagated artificially and have huge market development potential.Carry out stock enhancement and the industrialization cultivation of C. guichenoti, first must break through its artificial propagation techniques, realize the batch production of seed.C. guichenoti artificial induced spawning technology is then the key of artificial propagation techniques.Current domestic You Duojia research unit has carried out the research of C. guichenoti artificial propagation techniques simultaneously, but there is not yet its artificial induced spawning technology report.
Summary of the invention
The object of this invention is to provide a kind of C. guichenoti induced spawning method, realize the C. guichenoti artificial propagation under full manual control condition.
The technical solution adopted in the present invention is, a kind of C. guichenoti induced spawning method, specifically implements according to following steps:
(1), parent population is selected: select to cultivate ripe C. guichenoti from closed recirculating cultivating system and hasten parturition with parent population; Female-male proportion is: 1:2, and require that parent population is anosis without wound, physique is healthy and strong;
(2), oxytocic drug preparation and injection: oxytocic drug select luteotropin releasing hormone d-ala analog, DOM and 0.7% fish physiological saline; Medicine divides 2 injections, during water temperature 19 DEG C, and two pin spacings 10 hours; First pin raun dosage is 1 μ g luteotropin releasing hormone d-ala analog+0.2mL0.7% physiological saline/kg fish body weight, and milter is not injected; Second pin raun dosage is 6 μ g luteotropin releasing hormone d-ala analog+3mg DOM+0.2mL0.7% physiological saline/kg fish body weight, and milter dosage is the half of raun dosage, and the parent population of injection oxytocic drug puts into preprepared spawning inducing pool;
(3), spawning inducing pool and Water-quality control: spawning inducing pool is flowing water pond, and area is generally 5-50 square metre, and select the spawning inducing pool of suitable size according to the hasten parturition quantity of parent population of single, parent population is placed density and is less than 0.5kg/m
2; Put into the spawning inducing pool water body after parent population and keep miniflow, flow control is at 0.1-0.2m/s, and water temperature controls at 19-22 DEG C, and oxygen in water remains on more than 8mg/L, and pH is between 7.0-8.2;
(4), artificial insemination: after arrival Effect time, check the ovulation ejaculation situation of male and female parent population, when water temperature 19 DEG C, its Effect time is 20 hours, when the ovulation of discovery raun, during milter ejaculation phenomenon, carries out artificial insemination immediately; Insemination procedures is dry method fertilization, first fish for and wait to produce raun, towel off dry fish body moisture, ovum is got in drying receptacle, then fish for and wait to produce milter, dry gonopore, seminal fluid is clamp-oned on ovum, stir gently with dry feather and rapidly seminal fluid is mixed with ovum, then activate with clear water, leave standstill after 2 minutes, pour fertilized egg into incubator and hatch;
(5), hatch: fertilized egg selects taper hatching pail to hatch, the ovum density of putting of hatching pail is 400-500 grain/L water body, and in hatching pail, water volume flow rate should regulate and control in good time, and before fertilized egg demoulding, flow velocity is 0.4m/s, after demoulding, flow velocity is 0.2m/s, controls hatching water quality consistent with spawning inducing pool water quality; After demoulding in hatching pail through the growth of 4 days, namely remove hatching pail and carry out seed rearing to the inflation of prelarva fish glue.
Feature of the present invention is also,
The Sex judging method of male and female C. guichenoti is as follows: in mating season, ripe milter pectoral fin adularescent pearl star, and feel is coarse, ripe raun pectoral fin without pearl star, smooth surface.
Parent population maturity determination methods is as follows: the feature that milter maturity is good is, in water, light pressure parent population belly, have seminal fluid to flow out, and namely chance water falls apart; After drawing the activation of a small amount of seminal fluid water with dry suction pipe, examine under a microscope sperm viability, have 25-35 Acute movement time second; The feature that raun maturity is good is, fish body belly expands, and ovary profile is obvious, and gonopore reddens; A small amount of ovum sample is taken out from parent population gonopore with digging egg apparatus, observe soak 3 minutes by transparent liquid in culture dish after, ovum grain answers size neat, full, whole or most of ovum grain egg nucleus off normal, polarization value and egg nucleus to the beeline of egg membrane and the ratio in ovum footpath between 1/10-1/14.
The component of transparent liquid is: turpentine alcohol 25 parts, 75% alcohol 50 parts, 25 parts, glacial acetic acid.
The invention has the beneficial effects as follows: under full manual control condition, by the method for manual injection's hormone induction, make that ripe C. guichenoti is laid eggs, ejaculation, artificial insemination method is adopted to obtain fertilized egg, and fry is hatched in incubation equipment, realize C. guichenoti artificial propagation, for technical foundation has been established in the stock enhancement of C. guichenoti and industrialization cultivation.
Embodiment
Below in conjunction with embodiment, the present invention is described in detail.
The invention provides a kind of C. guichenoti induced spawning method, specifically implement according to following steps:
(1), parent population is selected: first day is selected to cultivate 6 ripe tail C. guichenoti and hastened parturition with parent population, wherein 2 tail rauns, and 4 tail milters, parent population is anosis without wound, and physique is healthy and strong.
Sex judging and maturity judge as follows: 4 tail milter pectoral fin adularescent pearl stars, and feel is coarse, and in water, light pressure parent population belly, have seminal fluid to flow out, and namely chance water falls apart; After drawing the activation of a small amount of seminal fluid water with dry suction pipe, examine under a microscope sperm viability, have more than 25 seconds Acute movement times.2 tail raun pectoral fins without pearl star, smooth surface, fish body belly expands, and ovary profile is obvious, and gonopore reddens; Take out a small amount of ovum sample with digging egg apparatus from parent population gonopore, observe soak 3 minutes by transparent liquid in culture dish after, ovum grain size is neat, full, and most of ovum grain egg nucleus off normal, polarization value is about 1/10.
(2), oxytocic drug preparation and injection: oxytocic drug selects luteotropin releasing hormone d-ala analog (LRH-A
2), DOM (DOM) and fish be with physiological saline (0.7%NaCl).Medicine divides 2 injections, and first day 10:00 in the morning injects the first pin, and raun dosage is 1 μ gLRH-A
2+ 0.2mL0.7%NaCl/kg fish body weight, milter is not injected; First day 8:00 in afternoon injects the second pin, and raun dosage is 6 μ gLRH-A
2+ 3mg DOM+0.2mL0.7%NaCl/kg fish body weight, milter dosage is the half of raun dosage.The parent population of injection oxytocic drug puts into preprepared spawning inducing pool.
(3), spawning inducing pool and Water-quality control: spawning inducing pool is circular flowing water pond, area is 12.56 square metres, put into the spawning inducing pool water body after parent population and keep miniflow, flow control is between 0.1-0.2m/s, water temperature controls at 20 ± 0.5 DEG C, oxygen in water remains on more than 8mg/L, and pH is between 7.0-8.2.
(4), artificial insemination: the second afternoon 4:00 checks, find that raun has ovulation, milter has ejaculation phenomenon, carries out artificial insemination immediately.Insemination procedures is dry method fertilization, first fish for and wait to produce raun, towel off dry fish body moisture, ovum is got in drying receptacle, then fish for and wait to produce milter, dry gonopore, seminal fluid is clamp-oned on ovum, stir gently with dry feather and rapidly seminal fluid is mixed with ovum, then activate with clear water, leave standstill after 2 minutes, pour fertilized egg into incubator and hatch.2 tail rauns, for laying eggs 29000, obtain fertilized egg more than 4000 grain.
(5), hatch: fertilized egg selects taper hatching pail to hatch, and the ovum density of putting of hatching pail is 500/L water body.In hatching pail, water volume flow rate should regulate and control in good time, and before fertilized egg demoulding, flow velocity is 0.4m/s, and after demoulding, flow velocity is 0.2m/s, controls hatching water quality consistent with spawning inducing pool water quality.Fertilized egg, through 60.3 hours hatching rear demouldings, obtains membrane prelarva more than 200 tail altogether, after in hatching pail again through the growth of 4 days, remove hatching pail and carry out seed rearing to the inflation of prelarva fish glue.
Under full manual control condition, by the method for manual injection's hormone induction, make that ripe C. guichenoti is laid eggs, ejaculation, artificial insemination method is adopted to obtain fertilized egg, and fry is hatched in incubation equipment, realize C. guichenoti artificial propagation, for technical foundation has been established in the stock enhancement of C. guichenoti and industrialization cultivation.
Claims (4)
1. a C. guichenoti induced spawning method, is characterized in that, specifically implements according to following steps:
(1) parent population is selected: select to cultivate ripe C. guichenoti from closed recirculating cultivating system and hasten parturition with parent population; Female-male proportion is: 1:2, and require that parent population is anosis without wound, physique is healthy and strong;
(2) oxytocic drug preparation and injection: oxytocic drug select luteotropin releasing hormone d-ala analog, DOM and 0.7% fish physiological saline; Medicine divides 2 injections, during water temperature 19 DEG C, and two pin spacings 10 hours; First pin raun dosage is 1 μ g luteotropin releasing hormone d-ala analog+0.2mL0.7% physiological saline/kg fish body weight, and milter is not injected; Second pin raun dosage is 6 μ g luteotropin releasing hormone d-ala analog+3mg DOM+0.2mL0.7% physiological saline/kg fish body weight, and milter dosage is the half of raun dosage, and the parent population of injection oxytocic drug puts into preprepared spawning inducing pool;
(3) spawning inducing pool and Water-quality control: spawning inducing pool is flowing water pond, and area is generally 5-50 square metre, select the spawning inducing pool of suitable size according to the hasten parturition quantity of parent population of single, parent population is placed density and is less than 0.5kg/ ㎡; Put into the spawning inducing pool water body after parent population and keep miniflow, flow control is at 0.1-0.2m/s, and water temperature controls at 19-22 DEG C, and oxygen in water remains on more than 8mg/L, and pH is between 7.0-8.2;
(4) artificial insemination: after arrival Effect time, check the ovulation ejaculation situation of male and female parent population, when water temperature 19 DEG C, its Effect time is 20 hours, when the ovulation of discovery raun, during milter ejaculation phenomenon, carries out artificial insemination immediately; Insemination procedures is dry method fertilization, first fish for and wait to produce raun, towel off dry fish body moisture, ovum is got in drying receptacle, then fish for and wait to produce milter, dry gonopore, seminal fluid is clamp-oned on ovum, stir gently with dry feather and rapidly seminal fluid is mixed with ovum, then activate with clear water, leave standstill after 2 minutes, pour fertilized egg into incubator and hatch;
(5) hatch: fertilized egg selects taper hatching pail to hatch, the ovum density of putting of hatching pail is 400-500 grain/L water body, and in hatching pail, water volume flow rate should regulate and control in good time, and before fertilized egg demoulding, flow velocity is 0.4m/s, after demoulding, flow velocity is 0.2m/s, controls hatching water quality consistent with spawning inducing pool water quality; After demoulding in hatching pail through the growth of 4 days, namely remove hatching pail and carry out seed rearing to the inflation of prelarva fish glue.
2. C. guichenoti induced spawning method according to claim 1, is characterized in that, the Sex judging method of male and female C. guichenoti is as follows: in mating season, ripe milter pectoral fin adularescent pearl star, and feel is coarse, ripe raun pectoral fin without pearl star, smooth surface.
3. C. guichenoti induced spawning method according to claim 1, is characterized in that, parent population maturity determination methods is as follows: the feature that milter maturity is good is, in water, light pressure parent population belly, have seminal fluid to flow out, and namely chance water falls apart; After drawing the activation of a small amount of seminal fluid water with dry suction pipe, examine under a microscope sperm viability, have 25-35 Acute movement time second; The feature that raun maturity is good is, fish body belly expands, and ovary profile is obvious, and gonopore reddens; A small amount of ovum sample is taken out from parent population gonopore with digging egg apparatus, observe soak 3 minutes by transparent liquid in culture dish after, ovum grain answers size neat, full, whole or most of ovum grain egg nucleus off normal, polarization value and egg nucleus to the beeline of egg membrane and the ratio in ovum footpath between 1/10-1/14.
4. C. guichenoti induced spawning method according to claim 3, is characterized in that, the component of described transparent liquid is: turpentine alcohol 25 parts, 75% alcohol 50 parts, 25 parts, glacial acetic acid.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105409845A (en) * | 2015-12-01 | 2016-03-23 | 中国科学院昆明动物研究所 | Artificial reproduction method for schizothorax wangchiachii |
| CN106719131A (en) * | 2016-11-28 | 2017-05-31 | 山东省淡水渔业研究院 | A kind of propagation method suitable for sticking up mouth Culter family selective breedings |
| CN107581111A (en) * | 2017-11-08 | 2018-01-16 | 重庆市同盟农业开发有限公司 | A kind of Triploid Rainbow Trout fish seeding technique method |
| CN108064775A (en) * | 2018-02-11 | 2018-05-25 | 水利部中国科学院水工程生态研究所 | A kind of C. guichenoti artificial fecundation method |
| CN108186157A (en) * | 2018-02-09 | 2018-06-22 | 武汉中科瑞华生态科技股份有限公司 | A kind of artificial insemination method of C. guichenoti |
| CN109349170A (en) * | 2018-11-15 | 2019-02-19 | 宜昌三江渔业有限公司 | A kind of artificial cultivating method of C. guichenoti |
| CN110050729A (en) * | 2019-05-23 | 2019-07-26 | 吉林省水产科学研究院 | A kind of Tumen graining artificial fecundation method |
| CN110178761A (en) * | 2019-06-17 | 2019-08-30 | 江西省水产科学研究所 | A kind of induced spawning method of Wen Minnow |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105409845A (en) * | 2015-12-01 | 2016-03-23 | 中国科学院昆明动物研究所 | Artificial reproduction method for schizothorax wangchiachii |
| CN105409845B (en) * | 2015-12-01 | 2018-09-11 | 中国科学院昆明动物研究所 | A kind of short hairs schizothoracin artificial fecundation method |
| CN106719131A (en) * | 2016-11-28 | 2017-05-31 | 山东省淡水渔业研究院 | A kind of propagation method suitable for sticking up mouth Culter family selective breedings |
| CN107581111A (en) * | 2017-11-08 | 2018-01-16 | 重庆市同盟农业开发有限公司 | A kind of Triploid Rainbow Trout fish seeding technique method |
| CN108186157A (en) * | 2018-02-09 | 2018-06-22 | 武汉中科瑞华生态科技股份有限公司 | A kind of artificial insemination method of C. guichenoti |
| CN108064775A (en) * | 2018-02-11 | 2018-05-25 | 水利部中国科学院水工程生态研究所 | A kind of C. guichenoti artificial fecundation method |
| CN109349170A (en) * | 2018-11-15 | 2019-02-19 | 宜昌三江渔业有限公司 | A kind of artificial cultivating method of C. guichenoti |
| CN109349170B (en) * | 2018-11-15 | 2021-08-31 | 宜昌三江渔业有限公司 | Artificial breeding method for cupfish with round mouth |
| CN110050729A (en) * | 2019-05-23 | 2019-07-26 | 吉林省水产科学研究院 | A kind of Tumen graining artificial fecundation method |
| CN110178761A (en) * | 2019-06-17 | 2019-08-30 | 江西省水产科学研究所 | A kind of induced spawning method of Wen Minnow |
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