CN110050729A - A kind of Tumen graining artificial fecundation method - Google Patents
A kind of Tumen graining artificial fecundation method Download PDFInfo
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- CN110050729A CN110050729A CN201910432057.1A CN201910432057A CN110050729A CN 110050729 A CN110050729 A CN 110050729A CN 201910432057 A CN201910432057 A CN 201910432057A CN 110050729 A CN110050729 A CN 110050729A
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- 238000000034 method Methods 0.000 title claims abstract description 25
- 230000012447 hatching Effects 0.000 claims abstract description 19
- 235000013601 eggs Nutrition 0.000 claims abstract description 17
- 241000251468 Actinopterygii Species 0.000 claims abstract description 14
- 230000009027 insemination Effects 0.000 claims abstract description 8
- 230000000384 rearing effect Effects 0.000 claims abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 57
- 244000068988 Glycine max Species 0.000 claims description 17
- 235000010469 Glycine max Nutrition 0.000 claims description 17
- 235000013336 milk Nutrition 0.000 claims description 11
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- 210000004080 milk Anatomy 0.000 claims description 11
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- 210000004681 ovum Anatomy 0.000 claims description 9
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- 238000007689 inspection Methods 0.000 claims description 3
- 230000035800 maturation Effects 0.000 claims description 3
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- 235000012424 soybean oil Nutrition 0.000 claims description 3
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- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 238000003860 storage Methods 0.000 claims description 3
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- 150000003722 vitamin derivatives Chemical class 0.000 claims description 3
- 235000015099 wheat brans Nutrition 0.000 claims description 3
- DPWSRXJWCYEGIV-CWMZTGDLSA-N acetic acid;n-[(2s)-1-[[(2s)-1-[[(2s)-1-[[(2s)-1-[[(2r)-1-[[(2s)-1-[[(2s)-5-(diaminomethylideneamino)-1-[(2s)-2-(ethylcarbamoyl)pyrrolidin-1-yl]-1-oxopentan-2-yl]amino]-4-methyl-1-oxopentan-2-yl]amino]-1-oxopropan-2-yl]amino]-3-(4-hydroxyphenyl)-1-oxoprop Chemical compound CC(O)=O.CC(O)=O.CCNC(=O)[C@@H]1CCCN1C(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](C)NC(=O)[C@@H](NC(=O)[C@H](CO)NC(=O)[C@H](CC=1C2=CC=CC=C2NC=1)NC(=O)[C@H](CC=1NC=NC=1)NC(=O)C1NC(=O)CC1)CC1=CC=C(O)C=C1 DPWSRXJWCYEGIV-CWMZTGDLSA-N 0.000 claims description 2
- YYRMJZQKEFZXMX-UHFFFAOYSA-L calcium bis(dihydrogenphosphate) Chemical compound [Ca+2].OP(O)([O-])=O.OP(O)([O-])=O YYRMJZQKEFZXMX-UHFFFAOYSA-L 0.000 claims description 2
- 229940062672 calcium dihydrogen phosphate Drugs 0.000 claims description 2
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- 239000003513 alkali Substances 0.000 claims 1
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- 235000013372 meat Nutrition 0.000 claims 1
- 230000004720 fertilization Effects 0.000 abstract description 8
- 230000004083 survival effect Effects 0.000 abstract description 6
- 238000009360 aquaculture Methods 0.000 abstract description 2
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- QLGKMLRGKPKGKI-QWQCQAQZSA-N (2s)-n-[(2s)-1-[[(2s)-1-[[(2s)-1-[[(2s)-1-[[(2r)-1-[[(2s)-1-[[(2s)-5-(diaminomethylideneamino)-1-[(2s)-2-(ethylcarbamoyl)pyrrolidin-1-yl]-1-oxopentan-2-yl]amino]-4-methyl-1-oxopentan-2-yl]amino]-1-oxopropan-2-yl]amino]-3-(4-hydroxyphenyl)-1-oxopropan-2-yl Chemical compound CCNC(=O)[C@@H]1CCCN1C(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CC(C)C)NC(=O)[C@@H](C)NC(=O)[C@@H](NC(=O)[C@H](CO)NC(=O)[C@H](CC=1C2=CC=CC=C2NC=1)NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H]1NC(=O)CC1)CC1=CC=C(O)C=C1 QLGKMLRGKPKGKI-QWQCQAQZSA-N 0.000 description 3
- 230000032696 parturition Effects 0.000 description 3
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- PHIQHXFUZVPYII-ZCFIWIBFSA-O (R)-carnitinium Chemical compound C[N+](C)(C)C[C@H](O)CC(O)=O PHIQHXFUZVPYII-ZCFIWIBFSA-O 0.000 description 2
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- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- SQUHHTBVTRBESD-UHFFFAOYSA-N Hexa-Ac-myo-Inositol Natural products CC(=O)OC1C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C1OC(C)=O SQUHHTBVTRBESD-UHFFFAOYSA-N 0.000 description 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
- 241000594011 Leuciscus leuciscus Species 0.000 description 1
- 241000808802 Leuciscus waleckii tumensis Species 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 description 1
- 206010054949 Metaplasia Diseases 0.000 description 1
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-L Phosphate ion(2-) Chemical compound OP([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-L 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
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- 238000009835 boiling Methods 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 235000019784 crude fat Nutrition 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000001647 drug administration Methods 0.000 description 1
- 229960000367 inositol Drugs 0.000 description 1
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 229910052748 manganese Inorganic materials 0.000 description 1
- 239000011572 manganese Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000015689 metaplastic ossification Effects 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 229960003512 nicotinic acid Drugs 0.000 description 1
- 235000001968 nicotinic acid Nutrition 0.000 description 1
- 239000011664 nicotinic acid Substances 0.000 description 1
- 229940055726 pantothenic acid Drugs 0.000 description 1
- 235000019161 pantothenic acid Nutrition 0.000 description 1
- 239000011713 pantothenic acid Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Polymers & Plastics (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Environmental Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Biodiversity & Conservation Biology (AREA)
- Insects & Arthropods (AREA)
- Birds (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
A kind of Tumen graining artificial fecundation method belongs to technical field of aquaculture, including Tumen graining parent fish rearing, parent population selection, male and female parent population combo, artificial induced spawning, artificial insemination, fertilized eggs unsticking and hatching barrel hatching.The method can significantly improve the spawning rates of Tumen graining artificial propagation, the survival rate of rate of fertilization, hatching rate and artificial propagation fry；The blank of Tumen graining artificial fecundation method has been filled up, has provided a kind of feasible method for Tumen graining large-scale production, has there is certain economic significance and ecological significance to the population conservation for the Tumen graining for being distributed only over TUMENJIANG RIVER.
Description
Technical field
The invention belongs to technical field of aquaculture, especially relate to a kind of artificial fecundation method of Tumen graining.
Background technique
Tumen graining (Leuciscus waleckii tumensis Mori), the flesh of fish is pure white, delicate, fishiness is delicious,
It is one of " three Hua Wuluo " delicious fish in the Northeast.For the distinctive Species of Rare Fish from Qingdao in Changbaishan area.Due to excessively catching in recent years
Fishing, water conservancy projects barrier, Tumen graining biotic population quantity die-off.In order to restore the germ plasm resource of Tumen graining, need
A kind of new technical solution solves the problems, such as this.By the way of artificial propagation, achieve the purpose that proliferation, and improve artificial breeding
Grow the survival rate of Tumen graining seed.
Summary of the invention
The technical problems to be solved by the present invention are: a kind of Tumen graining artificial fecundation method is provided, using artificial breeding
The mode grown achievees the purpose that proliferation, and can significantly improve the spawning rate of Tumen graining artificial propagation, rate of fertilization, hatching rate
With the survival rate of artificial propagation fry.
A kind of Tumen graining artificial fecundation method, it is characterized in that: include the following steps, and following steps sequentially carry out,
Step 1: Tumen graining parent fish rearing
In First Year June~October, 3 ages~6 age maps graining milters and 4 ages~7 age maps grainings are chosen
Raun carries out artificial culture in flowing water pond, respectively feeds a feed sooner or later daily；
Step 2: parent population selects
Between second year April after step 1 artificial culture~May, water temperature stability is at 4 DEG C~6 DEG C, choosing
Well-developed Tumen graining is selected as artificial propagation parent population；
Step 3: male and female parent population combo
It with female-male proportion is that 3:1 point opens that move into area be in the flowing water pond of the ㎡ of 20 ㎡~25 by the parent population selected in step 2
It temporarily supports, depth of water 50cm~80cm, temporarily feeding density is less than 16kg/ ㎡, water flow velocity 10m/s~15m/s；
Step 4: artificial induced spawning
The parent population of gonadal maturation in selecting step three injects oxytocic drug in pectoral fin base portion；
Step 5: artificial insemination
After step 4 injects oxytocic drug 18h~for 24 hours, every the female of inspections in 1 hour and male parent population, light pressure is female
Parent population abdomen has ovum grain to flow out from gonopore, light to press male parent population abdomen, has sperm to flow out from gonopore, carries out artificial insemination,
Clear water is added after the female fish-egg grain of 3 tails is mixed with 1 tail milter sperm, stirs 20s~40s with feather；
Step 6: fertilized eggs unsticking
Fertilized eggs are washed with water 2 times~3 times, are added in soya-bean milk, after stirring 1min~2min, wash with water 3 times~5
It is secondary, after standing 4min~9min, soya-bean milk is added again, after stirring 1min~2min, sloughs viscosity；
Step 7: hatching barrel is hatched
Fertilized eggs after step 6 unsticking are put into cylindrical body hatching barrel and are hatched, putting ovum density is that every liter of water is put into fertilization
800~1000, ovum, oxygen in water is greater than 6mg/L, 4 DEG C~9 DEG C of water temperature, after hatching 15d~30d, hatches fry, carries out
Artificial culture.
Flowing water pond uses 1.5ppm~2.5ppm liquor potassic permanganate pair before putting into Tumen graining in the step 1
Carry out disinfection processing, and water depth is 0.8m~1.2m, and breeding density is less than 18kg/ ㎡, and water flow velocity is 4cm/s~10cm/
S, dissolved oxygen are not less than subject to 6mg/L, pH value 6.5~8.7, and 15 DEG C~28 DEG C of water temperature.
Fed in the step 1 feed ingredient be fish meal, dregs of beans, corn flour, rapeseed dregs, soybean, soybean oil, flour,
Wheat bran, calcium dihydrogen phosphate, choline, carnitine, glycine betaine, microelement and vitamin.
The oxytocic drug injected in the step 4 is luteinizing hormone releasing hormone LHRH-A2With dioxone DOM, wherein female
Fish per kilogram fish body weight injection dosage is luteinizing hormone releasing hormone LHRH-A210 μ of μ g/kg~15 g/kg and dioxone
DOM8mg/kg~12mg/kg, it is luteinizing hormone releasing hormone LHRH-A that milter per kilogram fish body, which reuses dose,25 μ g/kg~
7.5 μ g/kg and dioxone DOM4mg/kg~6mg/kg.
Fertilized eggs unsticking in the step 6 is after crushing soya bean 80g~95g, water 1.2L~1.5L to be added to boil with soya-bean milk
Boiling, retains spare when cooling to consistent with storage pond, holding pond water temperature.
Through the above design, the present invention can be brought the following benefits: a kind of Tumen graining artificial propagation side
Method can significantly improve the spawning rate of Tumen graining artificial propagation, the survival rate of rate of fertilization, hatching rate and artificial propagation fry；
The blank of Tumen graining artificial fecundation method has been filled up, has provided a kind of feasible method for Tumen graining large-scale production,
There are certain economic significance and ecological significance to the population conservation for the Tumen graining for being distributed only over TUMENJIANG RIVER.
Further beneficial effect of the invention is,
1, the spawning rate of Tumen graining parent population can reach 95% or more；
2, drug used in hastening parturition and dosage can make raun and milter Developmental maturity on point at the same time, synchronous hair
It educates, improves rate of fertilization, rate of fertilization is 98% or more；
3, soya-bean milk unsticking is used after inseminating, and avoids the damage to fertilized eggs, secondary unsticking avoids fertilized eggs in the incubation period
Between generate water mold, hatching rate is improved, up to 93% or more；And prevent from hatching fry deformity, improve survival rate of fish fry；
4, breeding water temperature is reduced, artificial propagation can be carried out 2 DEG C~4 DEG C of water temperature of time in the early spring, make supporting for fry
Time advance two weeks is grown, yield is increased, improves breeding efficiency.
Detailed description of the invention
Below in conjunction with the drawings and specific embodiments, the present invention is further illustrated:
Fig. 1 is a kind of Tumen graining artificial fecundation method schematic process flow diagram of the present invention.
Specific embodiment
A kind of Tumen graining artificial fecundation method, as shown in Figure 1, include the following steps,
Step 1: Tumen graining parent fish rearing:
In June~October of First Year, chooses the refined sieve milter of 3 ages~6 age maps and 4 ages~refined sieve of 7 age maps is female
Fish carries out artificial culture.
The entire nurturing period carries out in flowing water pond, and parent population, which enters before pond is cultivated, uses 1.5ppm~2.5ppm liquor potassic permanganate pair
Culture pond carries out disinfection processing.Water depth is 0.8m~1.2m.Breeding density be less than 18kg/ ㎡, water flow velocity be 4cm/s~
10cm/s, dissolved oxygen are not less than subject to 6mg/L, pH value 6.5~8.7, and 15 DEG C~28 DEG C of water temperature.
Mixed feed is fed, 2 times a day, once in the morning and once at night.
Mixed feed ingredient are as follows: gross protein value 33%~43%, crude fat 7.0%~9.0%, carbohydrate
43%~56%, lysine 1.90%~2.30%, methionine 0.60%~0.75%, calcium 0.45%~0.75%, total phosphorus
1.05%~1.20%.
Primary raw material composition are as follows: fish meal, dregs of beans, corn flour, rapeseed dregs, soybean, soybean oil, flour, wheat bran, biphosphate
Calcium, choline, carnitine, glycine betaine, microelement (copper, iron, zinc, manganese etc.), vitamin (A, D, E, K, C, niacin, pantothenic acid, inositol
Deng).
Step 2: parent population selects:
Second year April~May, water temperature stability at 4 DEG C~6 DEG C, select it is physical strong, without disease, scale and fin
Item is complete, the parent population without deformity, bright in color, well-developed Tumen graining as artificial propagation.
Step 3: male and female parent population combo:
It with female-male proportion is that 3 ﹕ 1 are separately moved into the flowing water pond that area is the ㎡ of 20 ㎡~25 and temporarily supported by selected parent population, water
Deep 50cm~80cm, temporarily feeding density is less than 16kg/ ㎡, water flow velocity 10m/s~15m/s.
Step 4: artificial induced spawning:
The parent population of gonadal maturation is selected, artificial induced spawning is carried out.The drug used of hastening parturition is luteinizing hormone releasing hormone
A2(LHRH-A2) and dioxone (DOM).By the way of pectoral fin base portion drug administration by injection.Raun per kilogram fish body weight injection dosage
For LHRH-A2: 10 μ of μ g/kg~15 g/kg and DOM:8mg/kg~12mg/kg；Milter per kilogram fish body reuses dose and compares raun
Reduce half are as follows: LHRH-A2: 5 μ of μ g/kg~7.5 g/kg and DOM:4mg/kg~6mg/kg.
Step 5: artificial insemination:
After injecting oxytocic drug 18h~for 24 hours, every the female of inspections in 1 hour and male parent population, light pressure female parent population abdomen,
There is ovum grain to flow out from gonopore；Light pressure male parent population abdomen carries out artificial insemination when having sperm to flow out from gonopore.By every 3 tail
The ovum grain of raun squeezes out, and the sperm of 1 tail milter squeezes out, and clear water is added after mixing, is stirred 20 seconds~40 seconds with feather.
Step 6: fertilized eggs unsticking:
After above-mentioned fertilized eggs wash with water 2 times~3 times, it is slowly added in soya-bean milk, it is clear with water after stirring 1 minute~2 minutes
It washes 3 times~5 times.After standing 4 minutes~9 minutes, secondary unsticking is carried out, is slowly added to soya-bean milk again, is stirred 1 minute~2 minutes
Afterwards, viscosity can be sloughed.The soya-bean milk is after crushing soya bean 80g~95g, to add water 1.2L~1.5L to boil, cool to storage pond, holding pond
It is spare when water temperature.
Step 7: cylindrical body hatching barrel is hatched:
Fertilized eggs after unsticking, which are put into hatching barrel, hatches.Put ovum density are as follows: every liter of water is put into fertilized eggs 800 in hatching barrel
Grain~1000.Micro- Lotic hatching, oxygen in water are greater than 6mg/L.4 DEG C~9 DEG C of water temperature, 15d~30d is needed to hatch fry.
A kind of Tumen graining artificial fecundation method through the invention, improves the spawning rate of Tumen graining parent population,
It can reach 95% or more.
Drug used in hastening parturition and dosage can make raun and milter Developmental maturity on point at the same time, synchronous hair
It educates, improves rate of fertilization, rate of fertilization is 98% or more.
Soya-bean milk unsticking is used after insemination, avoids the damage to fertilized eggs, secondary unsticking avoids fertilized eggs during incubation
Water mold is generated, hatching rate is improved, up to 93% or more；And prevent from hatching fry deformity, improve survival rate of fish fry.
Breeding water temperature is reduced, the present invention can carry out artificial propagation 2 DEG C~4 DEG C of water temperature of time in the early spring, make fry
Culturing time be advanced by two weeks, increase yield, improve breeding efficiency.
The method of the invention has filled up the blank of Tumen graining artificial fecundation method.For Tumen graining scale metaplasia
Production provides a kind of feasible method, to the population conservation for the Tumen graining for being distributed only over TUMENJIANG RIVER have certain economic significance and
Ecological significance.
Claims (5)
1. a kind of Tumen graining artificial fecundation method, it is characterized in that: include the following steps, and following steps sequentially carry out,
Step 1: Tumen graining parent fish rearing
In First Year June~October, 3 ages~6 age maps graining milters and 4 ages~7 age maps graining rauns are chosen,
Artificial culture is carried out in flowing water pond, respectively feeds a feed sooner or later daily；
Step 2: parent population selects
Between second year April after step 1 artificial culture~May, water temperature stability is at 4 DEG C~6 DEG C, selection hair
Good Tumen graining is educated as artificial propagation parent population；
Step 3: male and female parent population combo
By the parent population selected in step 2 with female-male proportion be 3:1 point open move into area be in the flowing water pond of the ㎡ of 20 ㎡~25 temporarily
It supports, depth of water 50cm~80cm, temporarily feeding density is less than 16kg/ ㎡, water flow velocity 10m/s~15m/s；
Step 4: artificial induced spawning
The parent population of gonadal maturation in selecting step three injects oxytocic drug in pectoral fin base portion；
Step 5: artificial insemination
After step 4 injects oxytocic drug 18h~for 24 hours, every the female of inspections in 1 hour and male parent population, light pressure female parent population
Abdomen has ovum grain to flow out from gonopore, light to press male parent population abdomen, has sperm to flow out from gonopore, artificial insemination is carried out, by 3
Clear water is added after mixing with 1 tail milter sperm in the female fish-egg grain of tail, stirs 20s~40s with feather；
Step 6: fertilized eggs unsticking
Fertilized eggs are washed with water 2 times~3 times, are added in soya-bean milk, after stirring 1min~2min, are washed with water 3 times~5 times, it is quiet
After setting 4min~9min, soya-bean milk is added again, after stirring 1min~2min, sloughs viscosity；
Step 7: hatching barrel is hatched
Fertilized eggs after step 6 unsticking are put into cylindrical body hatching barrel and are hatched, putting ovum density is that every liter of water is put into fertilized eggs
800~1000, oxygen in water is greater than 6mg/L, 4 DEG C~9 DEG C of water temperature, after hatching 15d~30d, hatches fry, carries out people
Work is cultivated.
2. a kind of Tumen graining artificial fecundation method according to claim 1, it is characterized in that: flowing water in the step 1
Pond is handled using 1.5ppm~2.5ppm liquor potassic permanganate carrying out disinfection before putting into Tumen graining, and water depth is 0.8m
~1.2m, breeding density are less than 18kg/ ㎡, and water flow velocity is 4cm/s~10cm/s, and dissolved oxygen is not less than subject to 6mg/L, pH
Value 6.5~8.7,15 DEG C~28 DEG C of water temperature.
3. a kind of Tumen graining artificial fecundation method according to claim 1, it is characterized in that: being fed in the step 1
The ingredient of feed is fish meal, dregs of beans, corn flour, rapeseed dregs, soybean, soybean oil, flour, wheat bran, calcium dihydrogen phosphate, choline, meat
Alkali, glycine betaine, microelement and vitamin.
4. a kind of Tumen graining artificial fecundation method according to claim 1, it is characterized in that: being injected in the step 4
Oxytocic drug be luteinizing hormone releasing hormone LHRH-A2With dioxone DOM, wherein injection dosage is raun per kilogram fish body again
Luteinizing hormone releasing hormone LHRH-A210 μ of μ g/kg~15 g/kg and dioxone DOM8mg/kg~12mg/kg, milter per kilogram
It is luteinizing hormone releasing hormone LHRH-A that fish body, which reuses dose,25 μ of μ g/kg~7.5 g/kg and dioxone DOM4mg/kg~6mg/
kg。
5. a kind of Tumen graining artificial fecundation method according to claim 1, it is characterized in that: in the step 6 by
Smart ovum unsticking is after crushing soya bean 80g~95g, to add water 1.2L~1.5L to boil, cool to and storage pond, holding pond water temperature one with soya-bean milk
Retain when cause spare.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111296333A (en) * | 2020-03-30 | 2020-06-19 | 辽宁省淡水水产科学研究院 | Crossbreeding method for female high-body elegans and male walnuts |
| CN111387099A (en) * | 2020-04-26 | 2020-07-10 | 陵水德林诚信水产养殖有限公司 | Artificial hatching method for east star spots |
| CN111990294A (en) * | 2019-12-31 | 2020-11-27 | 新疆维吾尔自治区水产科学研究所 | Original seed harvesting, domesticating and artificial propagation method of Aschersonia songorica |
| CN113261517A (en) * | 2021-05-31 | 2021-08-17 | 中国水产科学研究院黑龙江水产研究所 | Bacteriostatic debonding agent, preparation method thereof and method for improving artificial breeding hatchability of Atalantic malabaricus via bacteriostatic debonding agent |
| CN115191384A (en) * | 2022-07-08 | 2022-10-18 | 西双版纳云博水产养殖开发有限公司 | Artificial propagation method of diplopod |
| CN115250970A (en) * | 2022-07-08 | 2022-11-01 | 西双版纳云博水产养殖开发有限公司 | Artificial propagation method and system for Chinese knot fish |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20020073216A (en) * | 2001-03-14 | 2002-09-23 | 이원교 | method for producing fertilized egg of spotted flounder |
| CN102326693A (en) * | 2011-07-14 | 2012-01-25 | 山东六和集团有限公司 | Carp overwintering feed |
| KR20140127402A (en) * | 2013-04-24 | 2014-11-04 | 경상남도 | Embryo production method for mackerel |
| CN104145870A (en) * | 2014-08-27 | 2014-11-19 | 四川省农业科学院水产研究所 | Secondary debonding method for acipenser dabryanus |
| CN104221958A (en) * | 2014-08-19 | 2014-12-24 | 中国水产科学研究院长江水产研究所 | Artificial spawning method for coreius guichenoti |
| CN105638519A (en) * | 2015-12-30 | 2016-06-08 | 贵州大学 | Method for raising hatching rate of carassius auratus gibelio and survival rate of larvas |
| CN106879515A (en) * | 2017-04-12 | 2017-06-23 | 布尔津县额河特有冷水鱼繁育开发有限公司 | A kind of viscidity-removing method of body graining embryonated egg high |
| CN107258617A (en) * | 2017-06-29 | 2017-10-20 | 铜仁市凯盛水产养殖有限公司 | A kind of cultural method of Leuciscus waleckii |
-
2019
- 2019-05-23 CN CN201910432057.1A patent/CN110050729A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20020073216A (en) * | 2001-03-14 | 2002-09-23 | 이원교 | method for producing fertilized egg of spotted flounder |
| CN102326693A (en) * | 2011-07-14 | 2012-01-25 | 山东六和集团有限公司 | Carp overwintering feed |
| KR20140127402A (en) * | 2013-04-24 | 2014-11-04 | 경상남도 | Embryo production method for mackerel |
| CN104221958A (en) * | 2014-08-19 | 2014-12-24 | 中国水产科学研究院长江水产研究所 | Artificial spawning method for coreius guichenoti |
| CN104145870A (en) * | 2014-08-27 | 2014-11-19 | 四川省农业科学院水产研究所 | Secondary debonding method for acipenser dabryanus |
| CN105638519A (en) * | 2015-12-30 | 2016-06-08 | 贵州大学 | Method for raising hatching rate of carassius auratus gibelio and survival rate of larvas |
| CN106879515A (en) * | 2017-04-12 | 2017-06-23 | 布尔津县额河特有冷水鱼繁育开发有限公司 | A kind of viscidity-removing method of body graining embryonated egg high |
| CN107258617A (en) * | 2017-06-29 | 2017-10-20 | 铜仁市凯盛水产养殖有限公司 | A kind of cultural method of Leuciscus waleckii |
Non-Patent Citations (3)
| Title |
|---|
| 杨荣国 等: "勃氏雅罗鱼苗种繁育及成鱼养殖技术", 《河北渔业》 * |
| 逯云召 等: "黏性卵孵化实用技术研究", 《科学养鱼》 * |
| 龙敏: "高体雅罗鱼人工繁殖及苗种培育技术", 《渔业致富指南》 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111990294A (en) * | 2019-12-31 | 2020-11-27 | 新疆维吾尔自治区水产科学研究所 | Original seed harvesting, domesticating and artificial propagation method of Aschersonia songorica |
| CN111296333A (en) * | 2020-03-30 | 2020-06-19 | 辽宁省淡水水产科学研究院 | Crossbreeding method for female high-body elegans and male walnuts |
| CN111387099A (en) * | 2020-04-26 | 2020-07-10 | 陵水德林诚信水产养殖有限公司 | Artificial hatching method for east star spots |
| CN113261517A (en) * | 2021-05-31 | 2021-08-17 | 中国水产科学研究院黑龙江水产研究所 | Bacteriostatic debonding agent, preparation method thereof and method for improving artificial breeding hatchability of Atalantic malabaricus via bacteriostatic debonding agent |
| CN115191384A (en) * | 2022-07-08 | 2022-10-18 | 西双版纳云博水产养殖开发有限公司 | Artificial propagation method of diplopod |
| CN115250970A (en) * | 2022-07-08 | 2022-11-01 | 西双版纳云博水产养殖开发有限公司 | Artificial propagation method and system for Chinese knot fish |
| CN115250970B (en) * | 2022-07-08 | 2023-06-02 | 西双版纳云博水产养殖开发有限公司 | Artificial propagation method and system for Chinese nodakers |
| CN115191384B (en) * | 2022-07-08 | 2023-06-02 | 西双版纳云博水产养殖开发有限公司 | Artificial breeding method for double-hole fish |
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