CN115191384A - Artificial propagation method of diplopod - Google Patents
Artificial propagation method of diplopod Download PDFInfo
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Abstract
The invention discloses an artificial breeding method of diplopore fishes, which comprises the steps of arranging a hot wire flow velocity meter in a fish pond, dividing water area blocks of the fish pond by taking the hot wire flow velocity meter as a unit, obtaining a strong water flow sequence of the water area blocks by utilizing the flow velocity acquired by the hot wire flow velocity meter in real time, calculating the torrent index of each water area block according to the strong water flow sequence of each water area block, finally calculating and obtaining the hedging series by combining the torrent indexes of each water area block, analyzing the layout of the fish pond, screening out the water area blocks which do not meet the requirements, and improving the layout of the fish pond. Thereby ensuring that the water flow rate characteristic which is beneficial to the secretion of the gonadotropin of the diplopore fishes can be formed in the water domain block and improving the success rate of artificial propagation of the diplopore fishes.
Description
Technical Field
The invention belongs to the technical field of data acquisition and intelligent breeding, and particularly relates to an artificial breeding method of diploporus formosanus.
Background
The diplopore fish is a special species in Yunnan, is only seen in Meng sea and Meng wax in the downstream of the Yangtze river, is in rapid life, is highly evolved into a sucker, is an rare example of the environment adaptation of organisms, has great scientific research value in fish classification and comparative anatomy, is captured in spawning seasons along the river by fishermen, is reduced in quantity and is endangered to extinction, and therefore the artificial propagation of the diplopore fish has great significance. Various artificial propagation techniques disclosed at present are mature day by day, but no artificial propagation method specially aiming at diplopod fish exists, and the artificial propagation method which is commonly used in the industry is to breed parent fish in a hydrological environment which is similar to the living environment and is suitable for promoting the gonad development of the parent fish so as to carry out artificial insemination after the gonad development of the parent fish is mature.
However, the diplopore fishes are extremely sensitive to water flow in the hydrologic environment, parent fishes of the diplopore fishes lay eggs in streams when the rivers of 6-7 months swell, wherein the reproduction state of the diplopore fishes is greatly influenced by the change of the water flow speed of the diplopore fishes, the conventional hydrologic environment simulation method cannot accurately control the water flow speed or the water flow to simulate the due hydrologic characteristics, so that the diplopore fishes are difficult to reach the ideal reproduction state in the artificial reproduction process, and the gonadotropin stimulation of the parent fishes cannot reach the due level so that the success rate of the artificial reproduction is low. Therefore, as described in patent document CN201910769252.3, artificial propagation of diplopore fishes, or in reference aquatic science and technology information, 2016, 43 th period ecological reproduction test of lipped fishes, a culture fish pond capable of accurately controlling water flow rate is needed in the process of simulating the hydrological environment of the diplopore fish propagation period, so as to improve the success rate of artificial propagation of diplopore fishes.
Disclosure of Invention
The invention aims to provide an artificial propagation method for diplopod, which solves one or more technical problems in the prior art and at least provides a beneficial selection or creation condition.
In order to accomplish the above object, according to an aspect of the present invention, there is provided an artificial propagation method of diplopod, the method comprising the steps of:
s100, screening sexually mature diplopod in the living waters of the diplopod as parent fishes;
s200, arranging a hot wire flow meter in the fish pond;
s300, dividing the water area block of the fish culture pond by taking a hot wire flow velocity meter as a unit;
s400, acquiring a strong water flow sequence of a water area block through the flow velocity acquired by the hot wire flow velocity meter in real time;
s500, calculating the torrent index of each water area block according to the strong water flow sequence of each water area block;
s600, calculating and acquiring hedging levels and analyzing the layout of the culture fish pond by combining the torrent indexes of all the water area blocks, screening out the water area blocks which do not meet the requirements, and improving the layout of the culture fish pond;
s700, putting the parent fish into an improved culture fishpond for culturing;
s800, selecting parent fish for artificial propagation to obtain fertilized eggs;
and S900, hatching the fertilized eggs.
Further, in step S100, the method of screening sexually mature diplopod fish as parent fish in the water area where the diplopod fish lives is: the diplopodia are captured from Meng sea and Meng wax in the downstream of Lancang river, and female fish with body length in the range of [150mm,200mm ] and male fish with body length in the range of [100mm,150mm ] are selected as standby parent fish.
Further, in step S200, the method for arranging the hot wire flow velocity meter in the fish pond comprises the steps of taking the water depth of the fish pond as Dph, and respectively representing the positions with the water depths of 0.1Dph,0.5Dph and 0.9Dph by a shallow water level, a middle water level and a lower water level; arranging a set of flow velocity measuring devices in the culture fish pond at intervals of Dph along the water flow direction, wherein Dph is the length; the number of the flow velocity measuring devices arranged in the culture fish pond is Nset; wherein every velocity of flow survey device contains 6 heater velocity of flow meters, 6 heater velocity of flow meters arrange respectively in shallow water level, mid water level and the lower water level of breeding the fish pond both sides.
Further, in step S300, the method for dividing the water area of the culture fish pond by using the hot wire rheometer as a unit is as follows: taking a hot wire flow meter as a unit, dividing the fish pond into Nset multiplied by 6 water area blocks, namely, each water area block has equal area, the geometric center of each water area block is respectively provided with a hot wire flow meter, and one hot wire flow meter is used for measuring the flow rate of one water area block in the fish pond.
Further, in step S400, the method for obtaining the strong water flow sequence of the water area block through the flow velocity acquired by the hot wire current velocity meter in real time includes: setting a sequence as a strong water flow sequence SFLS, wherein each block can acquire a flow velocity Fv in real time through a hot wire flow velocity meter, if the value of the flow velocity obtained at a moment m is larger than the value which is multiplied by the Bs of the flow velocity obtained at the previous moment, the flow velocity obtained at the moment m is used as a strong water flow value SF, and the flow velocity at the moment and the moment are used as an element (m, SF) to be added into the strong water flow sequence SFLS; wherein Bs is a multiple of the set water flow rate, and the value range is between [1.5 and 4 ].
Further, in step S500, the method for calculating the torrent index of each water area block according to the strong current sequence of each water area block is as follows:
obtaining a strong water flow sequence SFLS within one minute of the kth water area block, and if time values m of a plurality of continuous elements are continuous in time sequence in the SFLS, using a sequence formed by arranging the elements according to the sequence of m in the elements as a current surge section; calculating the torrent index: for a water area with the sequence number k, taking the median of the strong water flow values of all elements in the inrush current section as a first strong water flow value, and taking the arithmetic mean of the first strong water flow values of all the inrush current sections in the strong water flow sequence as the torrent index ytk.
When simulating the hydrological environment that diplopore fish entered the reproductive stage, need pile up the stone in breeding the fish pond so that aquatic formation water flow rate changes or difference, the stone is piled up density or the size of stone and is all can influence water flow rate and change or difference, consequently need through carrying out the analysis to the water flow rate of each waters piece, and at the in-process of analysis, quantize the ability that rivers can obtain lasting strong rivers in unit interval through the torrent index.
Preferably, in order to further quantify the ability of the water flow to obtain continuous strong water flow in unit time through the riptide index, the method for calculating the riptide index may further be:
setting a variable as the turn-up degree NBs of the inrush section by taking the number of elements in the inrush section as the inrush section window lth,
if the lth =1, setting the value of the turning-up degree NBs of the inrush current section as Bs;
if the lth is more than or equal to 2, setting the strong water flow value of the ith 2 element in the inrush current section to be NBs ' times of the strong water flow value of the ith 2' element, wherein NBs ' is used as sub-turn-up degrees, obtaining lth-1 sub-turn-up degrees from the inrush current section, and calculating the arithmetic average value of the sub-turn-up degrees as the turn-up degree NBs of the inrush current section; wherein i2 is the serial number of the element in the inrush current segment, and i2 'is the last serial number of i2 (i 2' = i 2-1);
taking the difference between the maximum value and the minimum value of the SF values of all the elements in the same surge section as a surge section difference tdlv; calculating to obtain torrent of water area block with sequence number kIndex yt k ,
Where i1 is a cumulative variable, ct (k) represents the number of surge segments in a strong current sequence in one minute, NBs k,i1 And tdlv k,i1 Respectively representing the rising degree and the falling degree of the surge section of the i1 th surge section in the water area block with the sequence number k, and using e (NBs) k ) Represents the arithmetic mean of the degree of turnover of the individual surge segments of a water field block with sequence number k, tdlv k Representing the difference between the maximum value and the minimum value in the strong water flow values of each element of the strong water flow sequence within one minute of the water area block with the sequence number of k; tl k Representing the total number of times the flow rate was obtained from a hot wire rheometer measurement in one minute.
Further, in step S600, the torrent index of each water area block is combined, the hedging number is calculated, the layout of the fish culture pond is analyzed, and the water area blocks which do not meet the requirements are screened out and the layout of the fish culture pond is improved by the following method: each water area block obtains a torrent index in real time: acquiring the torrent index of each water area block with the linear distance within the range of 1.5-2Dph for one water area block, and taking the difference between the maximum value and the minimum value as a first flow difference value FYD;
obtaining each first flow difference value FYD of the same water area within one minute to form flow difference sequences FYDLs, taking the difference between each element in the flow difference sequences and an element obtained at the previous moment of the element as a flow difference domain YDZ at the moment, after each flow difference domain is obtained in the flow difference sequences, taking the absolute value of the ratio of the maximum value in each flow difference domain to the non-zero minimum value in the flow difference domain as a first ratio FRt, and respectively taking the moment when the flow difference domain obtains the maximum value and the moment when the flow difference domain obtains the minimum value as a first sampling moment and a second sampling moment; acquiring an arithmetic mean value of the first flow difference value at each moment between the first sampling time and the second sampling time as a second flow difference value SYD; calculating the hedging grade gk:
wherein i2 is an accumulation variable, and m (FYDLs) represents the arithmetic mean value of each element in the flow difference sequence; lt (FYDLs) represents the number of elements in the stream difference sequence, FYD i2 Represents the i2 nd element in the stream difference sequence! Is a factorial sign;
obtaining the hedging grades gk of each water area block, obtaining a set formed by the hedging grades gk of all the water area blocks within one minute as a hedging space akZone, and calculating the inert ripening risk p (gk) of one water area block c |akZone)，gk c Representing the number of hedging steps, p (gk), at the current time c | akZone) represents the current number of hedging levels gk c Probability of gaussian distribution in the hedge space akZone; if the value of the risk of the at least two water area blocks in the set of flow rate measuring device in the inert direction ripening is larger than 95%, the water area blocks corresponding to the flow rate measuring device need to be additionally provided with stones or increase the resistance of water flow, and if the value of the risk of the at least two water area blocks in the set of flow rate measuring device in the inert direction ripening is smaller than 5%, the water area blocks corresponding to the flow rate measuring device need to be reduced in stones or reduce the resistance of water flow.
According to the hedging series calculated according to the torrent indexes of all water area blocks in the culture fish pond, the hedging series is utilized to describe the suitable state of the water flow velocity in all water areas for breeding the parent fish of the diplopore fish in the early stage, so that the hedging series of all the water area blocks is further analyzed for the stacking or layout state of stones in the culture pond to calculate the inert ripening risk, the water area blocks which are unfavorable or not accordant with the secretion of the gonadotropin of the diplopore fish are identified and the layout of the water area blocks is adjusted, and the water flow velocity characteristic which is favorable for the secretion of the gonadotropin of the diplopore fish can be formed in the water area blocks.
Further, in step S700, the method of putting the parent fish into the improved culture fish pond for cultivation is: breeding female diplopodia and male diplopodia in a culture fish pond according to the proportion of 1.5, wherein the density is 45 pieces per square meter; and (3) putting the artificial compound feed, wherein the daily feed is 1-3% of the weight of the fish.
Further, in step S800, a parent fish is selected for artificial propagation to obtain a fertilized egg by a method comprising the steps of firstly performing hormone injection to the parent fish: carrying out first injection on female diplopore fish with an oxytocic of LRH-A2, wherein the injection amount is 0.5 mu g/kg, and then carrying out second injection on the female diplopore fish after 6-8 h, wherein the oxytocic is a mixed solution of LRH-A2 and domperidone, and the injection concentrations are respectively LRH-A2: 5 μ g/kg, domperidone: 5mg/kg; the injection amount of the male diplopod is half of that of the female diplopod, the injection is performed synchronously with the second injection of the female diplopod, and only one injection is performed;
then, when the parent fish after induced spawning is going to spawn in the high-tide of estrus, spawn collection and sperm collection are carried out, mature sperm and spawn are fertilized in a container, and fertilized eggs are transferred into an incubation pool for running water incubation.
The invention has the beneficial effects that: the invention provides an artificial propagation method of diplopod, which is characterized in that in the process of simulating the hydrological environment of the reproduction period of the diplopod, a culture fish pond capable of accurately controlling the water flow velocity is determined, the water flow velocity characteristic favorable for the secretion of gonadotropin of the diplopod is determined in a water area block so as to improve the success rate of the artificial propagation of the diplopod, and reference can be made to a device for improving the yield acceleration rate of parent gobio ventricosa or to the research on the artificial propagation and embryonic development of the alosa sapidissima in the patent No. CN201720641191.9 or the ocean science of 2012 and 36 (7) in the literature.
Drawings
The above and other features of the present invention will become more apparent by describing in detail embodiments thereof with reference to the attached drawings in which like reference numerals designate the same or similar elements, it being apparent that the drawings in the following description are merely exemplary of the present invention and other drawings can be obtained by those skilled in the art without inventive effort, wherein:
FIG. 1 is a flow chart of an artificial reproduction method of diplopod.
Detailed Description
The conception, the specific structure and the technical effects of the present invention will be clearly and completely described in conjunction with the embodiments and the accompanying drawings to fully understand the objects, the schemes and the effects of the present invention. It should be noted that the embodiments and features of the embodiments in the present application may be combined with each other without conflict.
Referring to fig. 1, a flow chart of an artificial propagation method of diplopod is shown, and the following describes an artificial propagation method of diplopod according to an embodiment of the invention with reference to fig. 1, the method comprises the following steps:
s100, screening sexually mature diplopod in the living waters of the diplopod as parent fishes;
s200, arranging a hot wire current meter in the arranged fish pond;
s300, dividing the water area block of the culture fish pond by taking a hot wire flow meter as a unit;
s400, acquiring a strong water flow sequence of a water area block through the flow velocity acquired by the hot wire flow velocity meter in real time;
s500, calculating the torrent index of each water area block according to the strong water flow sequence of each water area block;
s600, calculating and obtaining hedging levels and analyzing the layout of the fish pond by combining the torrent indexes of all the water area blocks, screening out the water area blocks which do not meet the requirements, and improving the layout of the fish pond;
s700, putting the parent fish into an improved culture fish pond for culturing;
s800, selecting parent fish for artificial propagation to obtain fertilized eggs;
and S900, hatching the fertilized eggs.
Further, in step S100, the method of screening sexually mature diplopod fish as parent fish in the waters where the diplopod fish live is: the diplopodia are captured from Meng sea and Meng wax in the downstream of Lancang river, and female fish with body length in the range of [150mm,200mm ] and male fish with body length in the range of [100mm,150mm ] are selected as standby parent fish.
Further, in step S200, the method for arranging the hot wire flow velocity meter in the fish pond comprises the steps of taking the water depth of the fish pond as Dph, and respectively representing the positions with the water depths of 0.1Dph,0.5Dph and 0.9Dph by using a shallow water level, a middle water level and a lower water level; arranging a set of flow velocity measuring devices in the culture fish pond along the water flow direction at intervals of Dph, wherein Dph is the length; the number of the flow velocity measuring devices arranged in the culture fish pond is Nset; wherein every velocity of flow survey device contains 6 hot wire velocity of flow appearance, 6 hot wire velocity of flow appearance arrange respectively in the shallow water level, the intermediate water level and the lower water level of breeding the fish pond both sides.
Further, in step S300, the method for dividing the water area of the culture fish pond by using the hot wire rheometer as a unit is as follows: the method is characterized in that a hot wire flow meter is used as a unit to divide the fish pond into Nset multiplied by 6 water area blocks, namely, the area of each water area block is equal, the geometric center of each water area block is respectively provided with a hot wire flow meter, and the flow rate of one water area block in the fish pond is measured by one hot wire flow meter.
Further, in step S400, the method for obtaining the strong water flow sequence of the water area block through the flow velocity acquired by the hot wire current velocity meter in real time includes: setting a sequence as a strong water flow sequence SFLS, wherein each block can acquire a flow velocity Fv in real time through a hot wire flow velocity meter, if the value of the flow velocity obtained at a moment m is larger than the value which is multiplied by the Bs of the flow velocity obtained at the previous moment, the flow velocity obtained at the moment m is used as a strong water flow value SF, and the flow velocity at the moment and the moment are used as an element (m, SF) to be added into the strong water flow sequence SFLS; wherein Bs is a multiple of the set water flow rate, and the value range is [1.5,4 ].
Further, in step S500, the method for calculating the torrent index of each water area block according to the strong current sequence of each water area block is as follows:
obtaining a strong water flow sequence SFLS within one minute of the kth water area block, and if time values m of a plurality of continuous elements are continuous in time sequence in the SFLS, using a sequence formed by arranging the elements according to the sequence of m in the elements as a current surge section; the method for calculating the torrent index comprises the following steps: for a water area block with the sequence number k, taking the median of the strong water flow values of all elements in the inrush current section as a first strong water flow value, and calculating the first strong water flow value of each inrush current section in the strong water flow sequenceThe mean value of the operation is taken as the torrent index yt k 。
Preferably, in order to further quantify the ability of the water flow to obtain continuous strong water flow in unit time through the torrent index, the method for calculating the torrent index can also be as follows:
setting a variable as the turn-up degree NBs of the inrush section by taking the number of elements in the inrush section as the inrush section window lth,
if the lth =1, setting the value of the turning-up degree NBs of the inrush current section as Bs;
if the lth is more than or equal to 2, setting the strong water flow value of the ith 2 element in the inrush current section to be NBs ' times of the strong water flow value of the ith 2' element, wherein the NBs ' is used as a sub-turn-up degree, obtaining lth-1 sub-turn-up degrees from the inrush current section, and calculating the arithmetic mean value of each sub-turn-up degree as the turn-up degree NBs of the inrush current section; wherein i2 is the serial number of the element in the inrush current segment, and i2 'is the last serial number of i2 (i 2' = i 2-1);
taking the difference between the maximum value and the minimum value of the SF values of all the elements in the same surge section as a surge section difference tdlv; calculating to obtain the torrent index yt of the water area block with the sequence number of k k ,
Where i1 is a cumulative variable, ct (k) represents the number of surge segments in a strong current sequence in one minute, NBs k,i1 And tdlv k,i1 Respectively representing the rising degree and the falling height of the i1 st surge section in the water area block with the sequence number k, and using e (NBs) k ) Represents the arithmetic mean, tdlv, of the degree of turnover of each surge segment of a water domain block with sequence number k k Represents the difference between the maximum value and the minimum value among the strong water flow values of each element of the strong water flow sequence within one minute of the water area block with the sequence number k; tl k Representing the total number of times the filament rheometer measured the flow rate in one minute.
Further, in step S600, in combination with the torrent index of each water area block, calculating hedging levels and analyzing the layout of the fish culture pond, screening out water areas which do not meet the requirements and improving the layout of the fish culture pond, the method comprises the following steps: each water area block obtains a torrent index in real time: acquiring the torrent index of each water area block with the linear distance within the range of 1.5-2Dph for one water area block, and taking the difference between the maximum value and the minimum value as a first flow difference value FYD;
obtaining each first flow difference value FYD of the same water area within one minute to form flow difference sequences FYDLs, taking the difference between each element in the flow difference sequences and an element obtained at the previous moment of the element as a flow difference domain YDZ at the moment, after each flow difference domain is obtained in the flow difference sequences, taking the absolute value of the ratio of the maximum value in each flow difference domain to the non-zero minimum value in the flow difference domain as a first ratio FRt, and respectively taking the moment when the flow difference domain obtains the maximum value and the moment when the flow difference domain obtains the minimum value as a first sampling moment and a second sampling moment; acquiring an arithmetic mean value of first stream difference values at each moment between the first different-sample moment and the second different-sample moment as a second stream difference value SYD; calculating the hedging grade gk:
wherein i2 is an accumulation variable, and m (FYDLs) represents the arithmetic mean value of each element in the flow difference sequence; lt (FYDLs) represents the number of elements in the stream difference sequence, FYD i2 Represents the i2 nd element in the stream difference sequence! Is a factorial sign;
obtaining the hedging grades gk of each water area block, obtaining a set formed by the hedging grades gk of all the water area blocks within one minute as a hedging space akZone, and calculating the inert ripening risk p (gk) of one water area block c |akZone)，gk c Representing the number of hedging steps, p (gk), at the current time c Ak zone) represents the current number of hedging levels gk c Probability of gaussian distribution in the hedge space akZone; if the value of the risk of the inertness-direction ripening of at least two water area blocks in one set of flow speed measuring device is larger than 95%, the water area blocks corresponding to the flow speed measuring device need to be additionally provided with stones or increase the resistance of water flow, and if the value of the risk of the inertness-direction ripening of at least two water area blocks in one set of flow speed measuring device is smaller than 5And percent, the water area corresponding to the flow velocity measuring device needs to reduce stones or reduce the resistance of water flow.
Further, in step S700, the method of putting the parent fish into the improved culture fish pond for cultivation includes: stocking the female diplopore fishes and the male diplopore fishes into a culture fish pond according to the proportion of 1.5, wherein the density is 45 fish per square meter; and (3) putting the artificial compound feed, wherein the daily feed is 1-3% of the weight of the fish.
Further, in step S800, the method for obtaining fertilized eggs by selecting parent fish for artificial propagation comprises the following steps: and (2) carrying out first injection of an oxytocic on female diplopod, wherein the oxytocic is LRH-A2, the injection amount is 0.5 mu g/kg, and after 6-8 h later, carrying out second injection of the oxytocic on the female diplopod, and the oxytocic is a mixed solution of LRH-A2 and domperidone, wherein the injection concentrations are LRH-A2: 5 μ g/kg, domperidone: 5mg/kg; the injection amount of the male diplopod is half of that of the female diplopod, the injection is performed synchronously with the second injection of the female diplopod, and only one injection is performed;
then, when the parent fish after induced spawning is going to spawn in the high-tide of estrus, spawn collection and sperm collection are carried out, mature sperm and spawn are fertilized in a container, and fertilized eggs are transferred into an incubation pool for running water incubation.
The artificial propagation system for the diplopore fishes can be operated in computing equipment such as desktop computers, notebook computers, palm computers and cloud servers. The artificial propagation system for the diplopore fish can be operated by a system comprising, but not limited to, a processor and a memory. It will be appreciated by those skilled in the art that the example is merely an example of an artificial reproduction system for a two-hole fish, and does not constitute a limitation of an artificial reproduction system for a two-hole fish, and may include more or less components than a certain number of components, or some components in combination, or different components, for example, the artificial reproduction system for a two-hole fish may further include input and output devices, network access devices, buses, or the like.
The Processor may be a Central Processing Unit (CPU), other general purpose Processor, a Digital Signal Processor (DSP), an Application Specific Integrated Circuit (ASIC), a Field Programmable Gate Array (FPGA) or other Programmable logic device, discrete Gate or transistor logic, discrete hardware components, etc. The general purpose processor may be a microprocessor or the processor may be any conventional processor or the like, the processor being the control center of the artificial propagation system operation system for a kind of diplopore fish, and various interfaces and lines are used to connect the various parts of the whole artificial propagation system operation system for a kind of diplopore fish.
The memory may be used for storing the computer programs and/or modules, and the processor may be used for implementing the various functions of the artificial reproduction system for a kind of diplopore fish by running or executing the computer programs and/or modules stored in the memory and calling the data stored in the memory. The memory may mainly include a storage program area and a storage data area, wherein the storage program area may store an operating system, an application program required by at least one function (such as a sound playing function, an image playing function, etc.), and the like; the storage data area may store data (such as audio data, a phonebook, etc.) created according to the use of the cellular phone, and the like. In addition, the memory may include high-speed random access memory, and may also include non-volatile memory, such as a hard disk, a memory, a plug-in hard disk, a Smart Media Card (SMC), a Secure Digital (SD) Card, a Flash memory Card (Flash Card), at least one magnetic disk storage device, a Flash memory device, or other volatile solid state storage device.
Although the description of the present invention has been presented in considerable detail and with reference to a few illustrated embodiments, it is not intended to be limited to any such detail or embodiment or any particular embodiment so as to effectively encompass the intended scope of the invention. Furthermore, the foregoing describes the invention in terms of embodiments foreseen by the inventor for which an enabling description was available, notwithstanding that insubstantial modifications of the invention, not presently foreseen, may nonetheless represent equivalents thereto.
Claims (9)
1. An artificial propagation method of diplopod, which is characterized by comprising the following steps:
s100, screening sexually mature diplopodia in the living waters of the diplopodia as parent fishes;
s200, arranging a hot wire current meter in the arranged fish pond;
s300, dividing the water area block of the culture fish pond by taking a hot wire flow meter as a unit;
s400, acquiring a strong water flow sequence of a water area block through the flow velocity acquired by the hot wire flow velocity meter in real time;
s500, calculating the torrent index of each water area block according to the strong water flow sequence of each water area block;
s600, calculating and acquiring hedging levels and analyzing the layout of the culture fish pond by combining the torrent indexes of all the water area blocks, screening out the water area blocks which do not meet the requirements, and improving the layout of the culture fish pond;
s700, putting the parent fish into an improved culture fish pond for culturing;
s800, selecting parent fish for artificial propagation to obtain fertilized eggs;
and S900, hatching the fertilized eggs.
2. The artificial propagation method of diplopod according to claim 1, wherein in step S100, the method for screening sexually mature diplopod as parent fish in the water area where the diplopod live is: the method for screening sexually mature diplopod as parent fish in the water area where the diplopod live comprises the following steps: the diplopodia are captured from Meng sea and Meng wax in the lower reaches of Langchan river, and female fish with the body length in the range of [150mm,200mm ] and male fish with the body length in the range of [100mm,150mm ] are selected as standby parent fish.
3. The artificial propagation method of diplopore fish according to claim 1, wherein in step S200, the method of arranging the hot wire rheometer in the culture fish pond is to take the water depth of the culture fish pond as Dph, and the shallow water level, the middle water level and the lower water level represent the positions with the water depths of 0.1Dph,0.5Dph and 0.9Dph, respectively; arranging a set of flow velocity measuring devices every Dph distance or length in the fish culture pond along the water flow direction, wherein the number of the flow velocity measuring devices arranged in the fish culture pond is Nset; wherein every velocity of flow survey device contains 6 heater velocity of flow meters, 6 heater velocity of flow meters arrange respectively in shallow water level, mid water level and the lower water level of breeding the fish pond both sides.
4. The artificial propagation method of diplopore fish as claimed in claim 1, wherein in step S300, the method for dividing the water area of the culture fish pond by taking a hot wire flow meter as a unit is as follows: dividing the fish pond into Nset multiplied by 6 water area blocks by taking a hot wire flow meter as a unit, namely, each water area block has equal area and the geometric center of each water area block is respectively provided with a hot wire flow meter; the flow rate of a water area block in the culture fish pond is measured by a hot wire flow meter.
5. The artificial propagation method of diplopore fish as claimed in claim 1, wherein in step S400, the flow rate acquired in real time by the hot wire current meter is obtained by the following method of obtaining the strong water flow sequence of the water area block: setting a sequence as a strong water flow sequence SFLS, wherein each block can acquire a flow velocity Fv in real time through a hot wire flow velocity meter, if the value of the flow velocity obtained at a moment m is larger than the value which is multiplied by the Bs of the flow velocity obtained at the previous moment, the flow velocity obtained at the moment m is used as a strong water flow value SF, and the flow velocity at the moment and the moment are used as an element (m, SF) to be added into the strong water flow sequence SFLS; wherein Bs is a multiple of the set water flow rate, and the value range is between [1.5 and 4 ].
6. The artificial propagation method of diplopore fishes according to claim 1, wherein in step S500, the method for calculating the torrent index of each water area block according to the strong water flow sequence of each water area block is as follows:
k is used as the serial number of the water area block, and the value range of k is between 1 and the total amount of the water area block in the culture fish pond; to obtainIn the strong water flow sequence SFLS within one minute of the kth water area, if time values m of a plurality of continuous elements are continuous in time sequence in the SFLS, the elements are arranged according to the sequence of m in the elements to form a sequence as a current surge section; calculating the torrent index: for a water area with the sequence number k, taking the median of the strong water flow values of all elements in the inrush current section as a first strong water flow value, and taking the arithmetic mean of the first strong water flow values of all the inrush current sections in the strong water flow sequence as the torrent index yt k 。
7. The artificial propagation method of diplopore fish as claimed in claim 1, wherein in step S600, the torrent index of each water area block is combined, the hedging number is calculated and the layout of the culture fish pond is analyzed, and the water area blocks which do not meet the requirement are screened out and the layout of the culture fish pond is improved by: obtaining a torrent index in real time by each water area block: acquiring the torrent index of each water area block with the linear distance within the range of 1.5-2Dph for one water area block, and taking the difference between the maximum value and the minimum value as a first flow difference value FYD;
obtaining each first flow difference value FYD of the same water area within one minute to form flow difference sequences FYDLs, taking the difference between each element in the flow difference sequences and an element obtained at the previous moment of the element as a flow difference domain YDZ at the moment, after each flow difference domain is obtained in the flow difference sequences, taking the absolute value of the ratio of the maximum value in each flow difference domain to the non-zero minimum value in the flow difference domain as a first ratio FRt, and respectively taking the moment when the flow difference domain obtains the maximum value and the moment when the flow difference domain obtains the minimum value as a first sampling moment and a second sampling moment; acquiring an arithmetic mean value of first stream difference values at each moment between the first different-sample moment and the second different-sample moment as a second stream difference value SYD; calculating the hedging grade gk:
wherein i2 is an accumulation variable, and m (FYDLs) represents the arithmetic mean value of each element in the flow difference sequence; lt (FYD)Ls) represents the number of elements in the stream difference sequence, FYD i2 Representing the i2 th element in the flow difference sequence;
obtaining the hedging grades gk of each water area block, obtaining a set formed by the hedging grades gk of all the water area blocks within one minute as a hedging space akZone, calculating the overflow probability of one water area block as an inert-direction ripening risk p (gk) c |akZone)，p(gk c | akZone) is the hedging order gk c Probability value of Gaussian distribution in hedging space, wherein gk c Representing the hedging grade of the current time of the water area block; if the value of the risk of the at least two water area blocks in the set of flow rate measuring device in the inert direction ripening is larger than 95%, the water area blocks corresponding to the flow rate measuring device need to be additionally provided with stones or increase the resistance of water flow, and if the value of the risk of the at least two water area blocks in the set of flow rate measuring device in the inert direction ripening is smaller than 5%, the water area blocks corresponding to the flow rate measuring device need to be reduced in stones or reduce the resistance of water flow.
8. The artificial propagation method of diplopodia fish as claimed in claim 1, wherein in step S700, the method of putting parent fish into the improved culture fish pond for cultivation is: stocking the female diplopore fishes and the male diplopore fishes into a culture fish pond according to the proportion of 1.5, wherein the density is 45 fish per square meter; and (3) putting the artificial compound feed, wherein the daily feed is 1-3% of the weight of the fish.
9. The artificial propagation method of diplopod according to claim 1, wherein in step S800, the parent fish is selected for artificial propagation, and fertilized eggs are obtained by injecting hormones into the parent fish: and (2) carrying out first injection of an oxytocic on female diplopod, wherein the oxytocic is LRH-A2, the injection amount is 0.5 mu g/kg, and after 6-8 h later, carrying out second injection of the oxytocic on the female diplopod, wherein the oxytocic is a mixed solution of LRH-A2 and domperidone, and the injection concentrations are LRH-A2: 5 μ g/kg, domperidone: 5mg/kg; the injection amount of the male diplopod is half of that of the female diplopod, the injection is performed synchronously with the second injection of the female diplopod, and only one injection is performed;
then, when the parent fish after induced spawning is going to spawn in the high-tide of estrus, spawn collection and sperm collection are carried out, mature sperm and spawn are fertilized in a container, and fertilized eggs are transferred into an incubation pool for running water incubation.
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