CN115191384B - Artificial breeding method for double-hole fish - Google Patents
Artificial breeding method for double-hole fish Download PDFInfo
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Abstract
The invention discloses an artificial breeding method of double-hole fish, which comprises the steps of arranging a hot wire flow meter in a fish pond, dividing the fish pond into water areas by taking the hot wire flow meter as a unit, acquiring a strong water flow sequence of the water areas by utilizing the flow rate acquired by the hot wire flow meter in real time, calculating the torrent index of each water area according to the strong water flow sequence of each water area, finally combining the torrent index of each water area, calculating and acquiring the hedging number, analyzing the layout of the fish pond, screening out the water areas which do not meet the requirements, and improving the layout of the fish pond. Thereby ensuring that the water flow rate characteristics favorable for the gonadotrophin secretion of the double-hole fish can be formed in the water area blocks and improving the artificial breeding success rate of the double-hole fish.
Description
Technical Field
The invention belongs to the technical field of data acquisition and intelligent breeding, and particularly relates to an artificial breeding method of double-hole fish.
Background
The double-hole fish is a special species of Yunnan, only is found in sea and strong wax at the downstream of the lan cangjiang, the rapid flow life of the fish species and the high evolution of mouth are the suction cups, are an inadvisable example of biological adaptation in the environment, have great scientific research value in fish taxonomies and comparative anatomies, but are captured in spawning seasons along most of river fishermen, the number of the fish is greatly reduced, and the fish is endangered, so that the artificial propagation of the double-hole fish has great significance. Various artificial propagation techniques are disclosed to be mature day by day, but no artificial propagation method is specially aimed at the diplopore fish, and the conventional artificial propagation method in industry is to culture the parent fish in a hydrological environment which is similar to the living environment and is suitable for promoting the gonad development of the parent fish, so that the gonad development of the parent fish is mature, and then artificial insemination is carried out.
However, the double-hole fish is extremely sensitive to water flow in the hydrologic environment, parent fish of the double-hole fish spawns in stream when river water swells for 6-7 months, wherein the change of the water flow rate of the double-hole fish greatly influences the propagation state of the double-hole fish, the conventional hydrologic environment simulation method cannot accurately control the water flow speed or water flow rate to simulate the proper hydrologic characteristics, so that the double-hole fish is difficult to achieve the ideal propagation state in the artificial propagation process, and gonadotrophin stimulation of the parent fish cannot reach the required level, so that the success rate of artificial propagation is low. Therefore, as described in patent document CN201910769252.3, i.e. artificial breeding of double-hole fish, or in reference document aquatic science and technology information, 2016, 43, i.e. artificial breeding test of photopcheilium, there is a need for a fish pond capable of precisely controlling the water flow rate in the process of simulating the hydrologic environment of the breeding period of double-hole fish, so as to improve the success rate of artificial breeding of double-hole fish.
Disclosure of Invention
The invention aims to provide an artificial breeding method of double-hole fish, which aims to solve one or more technical problems in the prior art and at least provides a beneficial selection or creation condition.
In order to achieve the above object, according to an aspect of the present invention, there is provided a method for artificial propagation of a double-hole fish, the method comprising the steps of:
s100, screening sexually mature double-hole fish in water of double-hole fish life as parent fish;
s200, arranging a hot wire flow velocity meter in the arranged aquaculture fish pond;
s300, dividing water areas of the breeding fish pond by taking a hot wire flow velocity meter as a unit;
s400, acquiring a strong water flow sequence of the water area block through the flow velocity acquired in real time by the hot wire flow velocity meter;
s500, calculating the torrent index of each water area block according to the strong water flow sequence of each water area block;
s600, calculating and obtaining the hedging number by combining the torrent indexes of all the water area blocks, analyzing the layout of the aquaculture fish pond, screening out the water area blocks which do not meet the requirements and improving the layout of the aquaculture fish pond;
s700, putting parent fish into an improved fish culture pond for culture;
s800, selecting parent fish for artificial propagation to obtain fertilized eggs;
s900, hatching fertilized eggs.
Further, in step S100, the method of screening sexually mature double-hole fish as parent fish in the water in which the double-hole fish live is: double-hole fish were harvested from the Menghai and Mengha downstream of Lancangjiang, and female fish with body length in the range of [150mm,200mm ] and male fish with body length in the range of [100mm,150mm ] were selected as alternate parent fish.
Further, in step S200, the hot wire flow rate meter is arranged in the arrangement of the cultivation fish pond, wherein the water depth of the cultivation fish pond is Dph, and the shallow water level, the middle water level and the lower water level respectively represent the positions of 0.1Dph,0.5Dph and 0.9 Dph; arranging a set of flow velocity measuring devices at intervals of Dph in the water flow direction in the fish culture pond, wherein the Dph is the length; the number of the flow rate measuring devices arranged in the fish pond is Nset; each flow velocity measuring device comprises 6 hot wire flow velocity meters, and the 6 hot wire flow velocity meters are respectively arranged at the shallow water level, the medium water level and the lower water level at two sides of the fish pond.
Further, in step S300, the method for dividing the water area blocks of the cultured fish pond by using the hot filament current meter as a unit is as follows: the fishpond is divided into Nset multiplied by 6 water area blocks by taking a hot wire flow rate meter as a unit, namely, the area of each water area block is equal, the geometric center of each water area block is respectively provided with the hot wire flow rate meter, and the flow rate of one water area block in the fishpond is measured by the hot wire flow rate meter.
Further, in step S400, the method for obtaining the strong water flow sequence of the water area block through the flow rate acquired in real time by the hot wire flow meter is as follows: setting a sequence as a strong water flow sequence SFLS, wherein each block can acquire the flow velocity Fv in real time through a hot wire flow meter, if the value of the flow velocity obtained at a moment m is larger than the value of the flow velocity Bs times the flow velocity obtained at the moment m, the flow velocity obtained at the moment m is taken as a strong water flow value SF, and the moment and the flow velocity at the moment are taken as an element (m, SF) to be added into the strong water flow sequence SFLS; wherein Bs is a multiple of the set water flow rate, and the value range is between [1.5,4 ].
Further, in step S500, the method for calculating the torrent index of each water area block according to the strong water flow sequence of each water area block is as follows:
obtaining a strong water flow sequence SFLS within one minute of the kth water area block, wherein if the moment value m of a plurality of continuous elements in the SFLS is continuous in time sequence, the sequence formed by arranging the elements according to the sequence of m in the elements is used as a current section; calculating a torrent index: for the water area block with the serial number of k, taking the median among the strong water flow values of all elements in the strong water flow section as a first strong water flow value, and taking the arithmetic average value of the first strong water flow values of all the strong water flow sections in the strong water flow sequence as a torrent index ytk.
In the simulation of the hydrologic environment of the double-hole fish entering the breeding period, stones are required to be piled up in the breeding fish pond so as to form water flow speed change or difference, and the water flow speed change or difference is influenced by the rock piling density or the stone size, so that the water flow speed of each water area block needs to be analyzed, and in the analysis process, the capability of obtaining continuous strong water flow in unit time through the torrent index is quantified.
Preferably, in order to further quantify the ability of the water flow to obtain a continuous strong water flow in a unit time through the torrent index, the method for calculating the torrent index may be:
the number of elements in the inrush section is used as an inrush section window lth, a variable is set as the turning-up degree NBs of the inrush section,
if lth=1, setting the value of the turning-up degree NBs of the inrush current section as Bs;
if the lth is more than or equal to 2, setting the strong water current value of the i2 th element in the current section to be NBs ' times of the strong water current value of the i2' element, wherein NBs ' is used as sub-turnover degree, obtaining the lth-1 sub-turnover degree from the current section, and calculating the arithmetic average value of all sub-turnover degrees as the turnover degree NBs of the current section; wherein i2 is the sequence number of the element in the inrush segment, i2 'is the last sequence number of i2 (i 2' =i2-1);
with SF values of elements in the same inrush current segmentThe difference between the maximum value and the minimum value among them is taken as a surge section difference tdlv; calculating and obtaining the torrent index yt of the water area block with the serial number k k ,
Wherein i1 is an accumulated variable, ct (k) represents the number of inrush segments in the strong water flow sequence within one minute, NBs k,i1 And tdlv k,i1 Respectively representing the turn-up degree and the surge section fall of the ith 1 surge section in the water area block with the serial number k, and using e (NBs) k ) Arithmetic mean value of the rise of the individual gush segments, tdlv, representing the water area block with the sequence number k k The difference between the maximum value and the minimum value among the strong water flow values of each element of the strong water flow sequence within one minute of the water area block with the serial number k is represented; tl k Representing the total number of hot wire flow meter measurements to obtain flow rate in one minute.
Further, in step S600, in combination with the torrent index of each water area block, the hedging number is calculated and the layout of the fish pond is analyzed, and the method for screening out the water area blocks which do not meet the requirements and improving the layout of the fish pond is as follows: each water area block obtains the torrent index in real time: obtaining the torrent index of each water area block with the linear distance within the range of 1.5-2 Dph for one water area block, and taking the difference between the maximum value and the minimum value as a first flow difference value FYD;
obtaining each first flow difference value FYD within one minute of the same water area block to form a flow difference sequence FYDLs, taking the difference between each element in the flow difference sequence and the element obtained at the moment before the element as a flow difference domain YDZ at the moment, taking the absolute value of the ratio of the maximum value in each flow difference domain to the non-zero minimum value in the flow difference domain as a first ratio FRt after obtaining each flow difference domain in the flow difference sequence, and taking the moment when the flow difference domain obtains the maximum value and the moment when the flow difference domain obtains the minimum value as a first abnormal moment and a second abnormal moment respectively; acquiring an arithmetic average value of the first flow difference values at each time in the middle of the first abnormal time and the second abnormal time as a second flow difference value SYD; calculating the hedging level gk:
wherein i2 is an accumulated variable, and m (FYDLs) represents an arithmetic mean value of each element in the flow difference sequence; lt (FYDLs) represents the number of elements in the flow difference sequence, FYD i2 Represents the i2 nd element in the flow difference sequence +.! Is a factorial symbol;
obtaining the hedging number gk of each water area block, obtaining a set formed by the hedging numbers gk of all the water area blocks within one minute as a hedging space akZone, and calculating the idle ripening risk p (gk) of one water area block c |akZone)，gk c Represents the number of opposite-impact steps at the current time, p (gk) c I akZone) represents the current number of opposite-impact gk c Probability of gaussian distribution in the hedging space akZone; if the value of the idle ripening risk of at least two water area blocks in one set of flow rate measuring device is more than 95%, stones or resistance to water flow needs to be added or added to the water area blocks corresponding to the flow rate measuring device, and if the value of the idle ripening risk of at least two water area blocks in one set of flow rate measuring device is less than 5%, stones or resistance to water flow needs to be reduced to the water area blocks corresponding to the flow rate measuring device.
According to the hedging level calculated by the torrent index of each water area block in the aquaculture fish pond, the hedging level is utilized to describe the proper state of the water flow velocity in each water area for the breeding early-stage cultivation of the parent fish of the double-hole fish, so that the hedging level of each water area block is further analyzed to further calculate the inert ripening risk of the stacking or layout state of stones in the aquaculture pond, the water area blocks which are unfavorable for or do not accord with the gonadotrophin secretion of the double-hole fish are identified, the layout of the water area blocks is adjusted, and further the water flow velocity characteristics which are favorable for the gonadotrophin secretion of the double-hole fish can be formed in the water area blocks.
Further, in step S700, the parent fish is put into the improved fish pond for cultivation by the following method: stocking female double-hole fish and male double-hole fish into a fish pond according to the proportion of 1:1.5, wherein the density is 45 feet per square meter; and (3) adding artificial compound feed, wherein the daily feed is 1% -3% of the weight of the fish.
Further, in step S800, the parent fish is selected for artificial reproduction, and the fertilized egg is obtained by first performing hormone injection on the parent fish: carrying out first injection of an oxytocic for female double-hole fish, wherein the oxytocic is LRH-A2, the injection amount is 0.5 mug/kg, and after 6-8 hours, carrying out second injection of the oxytocic for female double-hole fish, wherein the oxytocic is a mixed solution of LRH-A2 and domperidone, and the injection concentrations are LRH-A2: 5 μg/kg, domperidone: 5mg/kg; the injection quantity of the male double-hole fish is half of that of the female fish, the injection is synchronous with the second injection of the female double-hole fish, and only one injection is carried out;
and then, when the parent fish after hastening parturition is about to spawn in the oestrus climax, spawning and semen collection are carried out, so that the mature semen and spawn are fertilized in a container, and the fertilized spawn is moved into a hatching pond for running water hatching.
The beneficial effects of the invention are as follows: the invention provides an artificial breeding method of double-hole fish, which is characterized in that a breeding fish pond capable of accurately controlling the water flow speed is determined in the process of simulating the hydrologic environment of the breeding period of the double-hole fish, the success rate of artificial breeding of the double-hole fish is improved by ensuring that the water flow speed characteristics favorable for gonadotrophin secretion of the double-hole fish can be formed in a water area block, and reference can be made to a device for improving the productivity of parent fish like goby with the patent number of CN201720641191.9 or research on artificial breeding and embryo development of American hilsa herring with the reference of ocean science, 2012, 36 (7).
Drawings
The above and other features of the present invention will become more apparent from the detailed description of the embodiments thereof given in conjunction with the accompanying drawings, in which like reference characters designate like or similar elements, and it is apparent that the drawings in the following description are merely some examples of the present invention, and other drawings may be obtained from these drawings without inventive effort to those of ordinary skill in the art, in which:
fig. 1 is a flow chart showing an artificial propagation method of the double-hole fish.
Detailed Description
The conception, specific structure, and technical effects produced by the present invention will be clearly and completely described below with reference to the embodiments and the drawings to fully understand the objects, aspects, and effects of the present invention. It should be noted that, in the case of no conflict, the embodiments and features in the embodiments may be combined with each other.
Referring to fig. 1, which is a flowchart illustrating an artificial propagation method of a double-hole fish, an artificial propagation method of a double-hole fish according to an embodiment of the present invention will be described with reference to fig. 1, and the method includes the steps of:
s100, screening sexually mature double-hole fish in water of double-hole fish life as parent fish;
s200, arranging a hot wire flow velocity meter in the arranged aquaculture fish pond;
s300, dividing water areas of the breeding fish pond by taking a hot wire flow velocity meter as a unit;
s400, acquiring a strong water flow sequence of the water area block through the flow velocity acquired in real time by the hot wire flow velocity meter;
s500, calculating the torrent index of each water area block according to the strong water flow sequence of each water area block;
s600, calculating and obtaining the hedging number by combining the torrent indexes of all the water area blocks, analyzing the layout of the aquaculture fish pond, screening out the water area blocks which do not meet the requirements and improving the layout of the aquaculture fish pond;
s700, putting parent fish into an improved fish culture pond for culture;
s800, selecting parent fish for artificial propagation to obtain fertilized eggs;
s900, hatching fertilized eggs.
Further, in step S100, the method of screening sexually mature double-hole fish as parent fish in the water in which the double-hole fish live is: double-hole fish were harvested from the Menghai and Mengha downstream of Lancangjiang, and female fish with body length in the range of [150mm,200mm ] and male fish with body length in the range of [100mm,150mm ] were selected as alternate parent fish.
Further, in step S200, the hot wire flow rate meter is arranged in the arrangement of the cultivation fish pond, wherein the water depth of the cultivation fish pond is Dph, and the shallow water level, the middle water level and the lower water level respectively represent the positions of 0.1Dph,0.5Dph and 0.9 Dph; arranging a set of flow velocity measuring devices at intervals of Dph in the water flow direction in the fish culture pond, wherein the Dph is the length; the number of the flow rate measuring devices arranged in the fish pond is Nset; each flow velocity measuring device comprises 6 hot wire flow velocity meters, and the 6 hot wire flow velocity meters are respectively arranged at the shallow water level, the medium water level and the lower water level at two sides of the fish pond.
Further, in step S300, the method for dividing the water area blocks of the cultured fish pond by using the hot filament current meter as a unit is as follows: the fishpond is divided into Nset multiplied by 6 water area blocks by taking a hot wire flow rate meter as a unit, namely, the area of each water area block is equal, the geometric center of each water area block is respectively provided with the hot wire flow rate meter, and the flow rate of one water area block in the fishpond is measured by the hot wire flow rate meter.
Further, in step S400, the method for obtaining the strong water flow sequence of the water area block through the flow rate acquired in real time by the hot wire flow meter is as follows: setting a sequence as a strong water flow sequence SFLS, wherein each block can acquire the flow velocity Fv in real time through a hot wire flow meter, if the value of the flow velocity obtained at a moment m is larger than the value of the flow velocity Bs times the flow velocity obtained at the moment m, the flow velocity obtained at the moment m is taken as a strong water flow value SF, and the moment and the flow velocity at the moment are taken as an element (m, SF) to be added into the strong water flow sequence SFLS; wherein Bs is a multiple of the set water flow rate, and the value range is between [1.5,4 ].
Further, in step S500, the method for calculating the torrent index of each water area block according to the strong water flow sequence of each water area block is as follows:
obtaining a strong water flow sequence SFLS within one minute of the kth water area block, wherein if the moment value m of a plurality of continuous elements in the SFLS is continuous in time sequence, the sequence formed by arranging the elements according to the sequence of m in the elements is used as a current section; the method for calculating the torrent index comprises the following steps: for a pair ofThe water area block with the serial number of k takes the median among the strong water flow values of all elements in the strong water flow section as a first strong water flow value, and takes the arithmetic average value of the first strong water flow values of all the strong water flow sections in the strong water flow sequence as a torrent exponent yt k 。
Preferably, in order to further quantify the ability of the water flow to obtain a continuous strong water flow in a unit time through the torrent index, the method for calculating the torrent index may be:
the number of elements in the inrush section is used as an inrush section window lth, a variable is set as the turning-up degree NBs of the inrush section,
if lth=1, setting the value of the turning-up degree NBs of the inrush current section as Bs;
if the lth is more than or equal to 2, setting the strong water current value of the i2 th element in the current section to be NBs ' times of the strong water current value of the i2' element, wherein NBs ' is used as sub-turnover degree, obtaining the lth-1 sub-turnover degree from the current section, and calculating the arithmetic average value of all sub-turnover degrees as the turnover degree NBs of the current section; wherein i2 is the sequence number of the element in the inrush segment, i2 'is the last sequence number of i2 (i 2' =i2-1);
taking the difference between the maximum value and the minimum value among SF values of various elements in the same surge section as a surge section difference tdlv; calculating and obtaining the torrent index yt of the water area block with the serial number k k ,
Wherein i1 is an accumulated variable, ct (k) represents the number of inrush segments in the strong water flow sequence within one minute, NBs k,i1 And tdlv k,i1 Respectively representing the turn-up degree and the surge section fall of the ith 1 surge section in the water area block with the serial number k, and using e (NBs) k ) Arithmetic mean value of the rise of the individual gush segments, tdlv, representing the water area block with the sequence number k k The difference between the maximum value and the minimum value among the strong water flow values of each element of the strong water flow sequence within one minute of the water area block with the serial number k is represented; tl k Representing the total number of hot wire flow meter measurements to obtain flow rate in one minute.
Further, in step S600, in combination with the torrent index of each water area block, the hedging number is calculated and the layout of the fish pond is analyzed, and the method for screening out the water area blocks which do not meet the requirements and improving the layout of the fish pond is as follows: each water area block obtains the torrent index in real time: obtaining the torrent index of each water area block with the linear distance within the range of 1.5-2 Dph for one water area block, and taking the difference between the maximum value and the minimum value as a first flow difference value FYD;
obtaining each first flow difference value FYD within one minute of the same water area block to form a flow difference sequence FYDLs, taking the difference between each element in the flow difference sequence and the element obtained at the moment before the element as a flow difference domain YDZ at the moment, taking the absolute value of the ratio of the maximum value in each flow difference domain to the non-zero minimum value in the flow difference domain as a first ratio FRt after obtaining each flow difference domain in the flow difference sequence, and taking the moment when the flow difference domain obtains the maximum value and the moment when the flow difference domain obtains the minimum value as a first abnormal moment and a second abnormal moment respectively; acquiring an arithmetic average value of the first flow difference values at each time in the middle of the first abnormal time and the second abnormal time as a second flow difference value SYD; calculating the hedging level gk:
wherein i2 is an accumulated variable, and m (FYDLs) represents an arithmetic mean value of each element in the flow difference sequence; lt (FYDLs) represents the number of elements in the flow difference sequence, FYD i2 Represents the i2 nd element in the flow difference sequence +.! Is a factorial symbol;
obtaining the hedging number gk of each water area block, obtaining a set formed by the hedging numbers gk of all the water area blocks within one minute as a hedging space akZone, and calculating the idle ripening risk p (gk) of one water area block c |akZone)，gk c Represents the number of opposite-impact steps at the current time, p (gk) c I akZone) represents the current number of opposite-impact gk c Probability of gaussian distribution in the hedging space akZone; if there is at least two water area blocks in a set of flow rate measuring device, the risk of ripening in the inert direction is increasedIf the value of the inert ripening risk of at least two water area blocks in one set of flow rate measuring device is less than 5%, the water area blocks corresponding to the flow rate measuring device need to reduce stones or reduce the resistance of water flow.
Further, in step S700, the parent fish is put into the improved fish pond for cultivation by the following method: stocking female double-hole fish and male double-hole fish into a fish pond according to the proportion of 1:1.5, wherein the density is 45 feet per square meter; and (3) adding artificial compound feed, wherein the daily feed is 1% -3% of the weight of the fish.
Further, in step S800, the parent fish is selected for artificial reproduction, and the fertilized egg is obtained by first performing hormone injection on the parent fish: carrying out first injection of an oxytocic for female double-hole fish, wherein the oxytocic is LRH-A2, the injection amount is 0.5 mug/kg, and after 6-8 hours, carrying out second injection of the oxytocic for female double-hole fish, wherein the oxytocic is a mixed solution of LRH-A2 and domperidone, and the injection concentrations are LRH-A2: 5 μg/kg, domperidone: 5mg/kg; the injection quantity of the male double-hole fish is half of that of the female fish, the injection is synchronous with the second injection of the female double-hole fish, and only one injection is carried out;
and then, when the parent fish after hastening parturition is about to spawn in the oestrus climax, spawning and semen collection are carried out, so that the mature semen and spawn are fertilized in a container, and the fertilized spawn is moved into a hatching pond for running water hatching.
The artificial breeding system of the double-hole fish can be operated in computing equipment such as a desktop computer, a notebook computer, a palm computer, a cloud server and the like. The artificial propagation system of the double-hole fish can comprise, but is not limited to, a processor and a memory. It will be appreciated by those skilled in the art that the example is merely an example of an artificial breeding system for a double-hole fish, and is not limiting of an artificial breeding system for a double-hole fish, and may include more or less components than examples, or may combine some components, or different components, e.g., the artificial breeding system for a double-hole fish may further include an input-output device, a network access device, a bus, etc.
The processor may be a central processing unit (Central Processing Unit, CPU), other general purpose processors, digital signal processors (Digital Signal Processor, DSP), application specific integrated circuits (Application Specific Integrated Circuit, ASIC), field programmable gate arrays (Field-Programmable Gate Array, FPGA) or other programmable logic devices, discrete gate or transistor logic devices, discrete hardware components, or the like. The general-purpose processor may be a microprocessor or the processor may be any conventional processor or the like, and the processor is a control center of the artificial propagation system operation system of the double-hole fish, and various interfaces and lines are used for connecting various parts of the whole artificial propagation system operation system of the double-hole fish.
The memory may be used to store the computer program and/or module, and the processor may implement various functions of the artificial propagation system for double-hole fish by running or executing the computer program and/or module stored in the memory and invoking data stored in the memory. The memory may mainly include a storage program area and a storage data area, wherein the storage program area may store an operating system, an application program (such as a sound playing function, an image playing function, etc.) required for at least one function, and the like; the storage data area may store data (such as audio data, phonebook, etc.) created according to the use of the handset, etc. In addition, the memory may include high-speed random access memory, and may also include non-volatile memory, such as a hard disk, memory, plug-in hard disk, smart Media Card (SMC), secure Digital (SD) Card, flash Card (Flash Card), at least one disk storage device, flash memory device, or other volatile solid-state storage device.
Although the present invention has been described in considerable detail and with particularity with respect to several described embodiments, it is not intended to be limited to any such detail or embodiment or any particular embodiment so as to effectively cover the intended scope of the invention. Furthermore, the foregoing description of the invention has been presented in its embodiments contemplated by the inventors for the purpose of providing a useful description, and for the purposes of providing a non-essential modification of the invention that may not be presently contemplated, may represent an equivalent modification of the invention.
Claims (6)
1. An artificial propagation method of double-hole fish, which is characterized by comprising the following steps:
s100, screening sexually mature double-hole fish in water of double-hole fish life as parent fish;
s200, arranging a hot wire flow velocity meter in the arranged aquaculture fish pond;
s300, dividing water areas of the breeding fish pond by taking a hot wire flow velocity meter as a unit;
s400, acquiring a strong water flow sequence of the water area block through the flow velocity acquired in real time by the hot wire flow velocity meter;
s500, calculating the torrent index of each water area block according to the strong water flow sequence of each water area block;
s600, calculating and obtaining the hedging number by combining the torrent indexes of all the water area blocks, analyzing the layout of the aquaculture fish pond, screening out the water area blocks which do not meet the requirements and improving the layout of the aquaculture fish pond;
s700, putting parent fish into an improved fish culture pond for culture;
s800, selecting parent fish for artificial propagation to obtain fertilized eggs;
s900, incubating fertilized eggs;
in step S400, the method for obtaining the strong water flow sequence of the water area block through the flow velocity acquired by the hot wire flow velocity meter in real time is as follows: setting a sequence as a strong water flow sequence SFLS, wherein each block can acquire the flow velocity Fv in real time through a hot wire flow meter, if the value of the flow velocity obtained at a moment m is larger than the value of the flow velocity Bs times the flow velocity obtained at the moment m, the flow velocity obtained at the moment m is taken as a strong water flow value SF, and the moment and the flow velocity at the moment are taken as an element (m, SF) to be added into the strong water flow sequence SFLS; wherein Bs is a multiple of the set water flow rate, and the value range is between [1.5,4 ];
in step S500, the method for calculating the torrent index of each water area block according to the strong water flow sequence of each water area block is as follows:
k is used as the serial number of the water area blocks, and the value of k ranges from 1 to the total amount of the water area blocks in the fish pond; obtaining a strong water flow sequence SFLS within one minute of a kth water area block, wherein if the moment values m of a plurality of continuous elements are continuous in time sequence in the SFLS, the sequence formed by arranging the elements according to the sequence of m in the elements is used as a current surge section; calculating a torrent index: for a water area block with the serial number of k, taking the median among the strong water flow values of all elements in the strong water flow section as a first strong water flow value, and taking the arithmetic average value of the first strong water flow values of all the strong water flow sections in the strong water flow sequence as a torrent exponent yt k；
In step S600, in combination with the torrent index of each water area block, the hedging number is calculated and the layout of the fish pond is analyzed, and the method for screening out the water area blocks which do not meet the requirements and improving the layout of the fish pond is as follows: each water area block obtains the torrent index in real time: obtaining the torrent index of each water area block with the linear distance within the range of 1.5-2 Dph for one water area block, and taking the difference between the maximum value and the minimum value as a first flow difference value FYD;
obtaining each first flow difference value FYD within one minute of the same water area block to form a flow difference sequence FYDLs, taking the difference between each element in the flow difference sequence and the element obtained at the moment before the element as a flow difference domain YDZ at the moment, taking the absolute value of the ratio of the maximum value in each flow difference domain to the non-zero minimum value in the flow difference domain as a first ratio FRt after obtaining each flow difference domain in the flow difference sequence, and taking the moment when the flow difference domain obtains the maximum value and the moment when the flow difference domain obtains the minimum value as a first abnormal moment and a second abnormal moment respectively; acquiring an arithmetic average value of the first flow difference values at each time in the middle of the first abnormal time and the second abnormal time as a second flow difference value SYD; calculating the hedging level gk:
wherein i2 is an accumulated variable, and m (FYDLs) represents an arithmetic mean value of each element in the flow difference sequence; lt (FYDLs) represents the number of elements in the flow difference sequence, FYD i2 Representing the i2 nd element in the flow difference sequence;
obtaining the hedging number gk of each water area block, obtaining a set formed by the hedging numbers gk of all the water area blocks within one minute as a hedging space akZone, and calculating the overflow probability of one water area block as an idle ripening risk p (gk) c |akZone)，p(gk c I akZone) is the number of opposite-impact steps gk c Probability values of gaussian distribution in hedging space, where gk c Representing the number of opposite-impact stages of the water area block at the current moment; if the value of the idle ripening risk of at least two water area blocks in one set of flow rate measuring device is more than 95%, stones or resistance to water flow needs to be added or added to the water area blocks corresponding to the flow rate measuring device, and if the value of the idle ripening risk of at least two water area blocks in one set of flow rate measuring device is less than 5%, stones or resistance to water flow needs to be reduced to the water area blocks corresponding to the flow rate measuring device.
2. The artificial propagation method of a double-hole fish according to claim 1, wherein in step S100, the method of screening sexually mature double-hole fish as parent fish in water in which the double-hole fish live is: the method for screening the sexually mature double-hole fish as parent fish in the water field in which the double-hole fish live is as follows: double-hole fish were harvested from the Menghai and Mengha downstream of Lancangjiang, and female fish with body length in the range of [150mm,200mm ] and male fish with body length in the range of [100mm,150mm ] were selected as alternate parent fish.
3. The artificial propagation method of double-hole fish according to claim 1, wherein in the step S200, a hot wire flow meter is arranged in the arrangement of the cultivation fish pond, wherein the water depth of the cultivation fish pond is Dph, and the positions of the water depths of 0.1Dph,0.5Dph and 0.9Dph are represented by a shallow water level, a medium water level and a low water level respectively; arranging a set of flow velocity measuring devices in the fish culture pond at intervals of Dph along the water flow direction or at intervals of length, wherein the number of the flow velocity measuring devices arranged in the fish culture pond is Nset; each flow velocity measuring device comprises 6 hot wire flow velocity meters, and the 6 hot wire flow velocity meters are respectively arranged at the shallow water level, the medium water level and the lower water level at two sides of the fish pond.
4. The artificial propagation method of double-hole fish according to claim 1, wherein in step S300, the method for dividing the water area of the breeding fish pond by using the hot filament flow rate meter as a unit comprises the steps of: dividing the fish pond into Nset multiplied by 6 water area blocks by taking a hot wire flow rate meter as a unit, wherein the area of each water area block is equal, and the geometric center of each water area block is respectively provided with the hot wire flow rate meter; the flow rate of a water area block in the fish pond is measured by a hot wire flow rate meter.
5. The artificial propagation method of double-hole fish according to claim 1, wherein in step S700, the parent fish is put into the improved fish pond for cultivation, which comprises the steps of: stocking female double-hole fish and male double-hole fish into a fish pond according to the proportion of 1:1.5, wherein the density is 45 feet per square meter; and (3) adding artificial compound feed, wherein the daily feed is 1% -3% of the weight of the fish.
6. The artificial propagation method of double-hole fish according to claim 1, wherein in step S800, parent fish is selected for artificial propagation, and fertilized eggs are obtained by first performing hormone injection on the parent fish: carrying out first injection of an oxytocic for female double-hole fish, wherein the oxytocic is LRH-A2, the injection amount is 0.5 mug/kg, and after 6-8 hours, carrying out second injection of the oxytocic for female double-hole fish, wherein the oxytocic is a mixed solution of LRH-A2 and domperidone, and the injection concentrations are LRH-A2: 5 μg/kg, domperidone: 5mg/kg; the injection quantity of the male double-hole fish is half of that of the female fish, the injection is synchronous with the second injection of the female double-hole fish, and only one injection is carried out;
and then, when the parent fish after hastening parturition is about to spawn in the oestrus climax, spawning and semen collection are carried out, so that the mature semen and spawn are fertilized in a container, and the fertilized spawn is moved into a hatching pond for running water hatching.
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