CN107494344A - A kind of artificial culturing method of advanced fry of yellow catfish - Google Patents
A kind of artificial culturing method of advanced fry of yellow catfish Download PDFInfo
- Publication number
- CN107494344A CN107494344A CN201710643603.7A CN201710643603A CN107494344A CN 107494344 A CN107494344 A CN 107494344A CN 201710643603 A CN201710643603 A CN 201710643603A CN 107494344 A CN107494344 A CN 107494344A
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Classifications
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Abstract
The invention discloses a kind of artificial culturing method of advanced fry of yellow catfish, including：Parent fish rearing, parent population fortification, parent population hasten parturition, lay eggs and collected, hatch, fry enriched nutritive.Have the beneficial effect that：Stimulate Pelteobagrus fulvidraco pituitary gonadotropin cell to develop ahead of time and enter secretory activity, promote the differentiation of sexual gland, promote the development of sexual organ；The parent fish rearing stage, strengthen the supplement of foreign aid's nutrition, strengthen in bait containing protein, amino acid, fat, vitamin etc. necessary to yellow catfish growing development, and it is aided with phagostimulant, antioxidant, Chinese herbal medicine etc., the nutritional requirement needed for parent population growth and development can be met, promote the growth of Pelteobagrus fulvidraco parent population and gonad development；It is improved the content of polyunsaturated fatty acid in the artemia body after reinforcing, it is suitable as the open-mouthed bait of seedling, the rearing condition is advantageous to improve immunocompetence, stress ability, the antibacterial resistance against diseases of the Pelteobagrus fulvidraco young, improves its survival rate and growth rate.
Description
Technical field
The present invention relates to the artificial culture field of aquatic products fry, more particularly, to a kind of artificial culture side of advanced fry of yellow catfish
Method.
Technical background
Yellow forehead（sǎng）Fish, is Chang sections, a kind of fish of Pelteobagrus, and body is about 120-140cm, and the outside of belly is straight, after body
Half portion is flat-sided, and caudal peduncle is more elongated, and head is big and flat, and kiss is short, and circle is blunt, and upper and lower jaw is slightly isometric, and mouth is big, the next, in two face and palatine bone
Villous shape cingulum, eye is small, side position, must be 4 pairs, and after nose palpus end can extend eye, the upper jaw must be 1 pair, most long, and chin or cheek must be 2 pairs, compared with the upper jaw
Short, the exposed no squama of body of palpus, side line are complete.Omnivorous, dwell toy, shrimp, aquatic small insect and some invertebrates at staple food bottom
Deng spawning in, 4~May, parent population has the habit graved to nest and protect offspring.Pelteobagrus fulvidraco be also known as yellow cheek fish, yellow bone fish, yellow stickleback,
River Long Dun Catfish are distributed in the basins such as Yangtze River in China, the Yellow River, the Zhujiang River and Heilungkiang.After acquisition, fin, internal organ are removed, cleans and uses, property
Sweet taste.Energy Energy benefit taste, inducing diuresis to remove edema,《Medical center collection will》Air supporting of harnessing the river is swollen, i.e., matches somebody with somebody mung bean, garlic with Pelteobagrus fulvidraco, rotten with boiling,
Go fish to eat beans, and take juice to adjust Phytolacca acinosa end clothes.Tonification and purgation in combination, and to insufficiency of the spleen, turgor is notably particularly suitable for.
At present, common problem is that survival rate is low in Pelteobagrus fulvidraco seed rearing, and susceptible disease, this have impact on Pelteobagrus fulvidraco and supports
Grow the development of industry.In recent years, with the increase of domestic and international demand, the continuous expansion of Pelteobagrus fulvidraco commodity fish culture scale, yellow forehead
The rearing of fingerling of fish is also progressively promoted, but the seed rearing survival rate of Pelteobagrus fulvidraco is low, is turned into and is hindered Pelteobagrus fulvidraco cultural technique
Bottleneck.
Artificial breeding technique on advanced fry of yellow catfish has many methods, and prior art is as authorized public number
The B of CN 101263793 Chinese invention patent, discloses a kind of artificial propagation method for advanced fry of yellow catfish, the inventive method
After selecting parent population, drum is put into spawning pond as fish nest, will by the use of the container of inverted conical inner wall smooth as hatching barrel
Embryonated egg launches wherein logical Lotic hatching, hatches after seedling that temporarily foster 40-72 hours pack sale, with it is simple to operate, can advise
The advantages of modelling produces, but the inventive method carries out fortification not before spawning to parent population, not to the sexual gland of parent population
Development is catalyzed, thus its Gunther parent fish spawning rate is low, and fertilization egg quality is not high, the survival rate of seedling is influenceed, moreover, seedling incubates
It is placed in cement pit after going out and temporarily supports, fortification is not carried out to the open-mouthed bait of seedling, is unfavorable for the growth of seedling.
The content of the invention
The purpose of this method is to provide a kind of Huang cultivated parent population, carry out seedling open-mouthed bait fortification
The artificial culturing method of forehead fish fry.
The present invention is directed to the problem of being mentioned in background technology, and the technical scheme taken is：A kind of advanced fry of yellow catfish it is artificial
Breeding method, including：Parent fish rearing, parent population fortification, parent population hasten parturition, lay eggs and collected, hatch, fry enriched nutritive, tool
Body comprises the following steps：
Parent fish rearing：The water quality in Pelteobagrus fulvidraco parent fish rearing pond will keep clear fresh pollution-free, and the dissolved oxygen amount of water is maintained at 3.5-
8mg/L, pH value 6.5-8.5, first -2 months 1 month to be hastened parturition in preparation, will wash by water 1 time, wash by water every time for every -10 days 7 days
Time is 50-80 minutes, and during bath, punching note can be carried out using the water in former pond, and parent population selection requires male 350-400g,
Female individuals 150-200g；Bath makes to form current in culture pond, can stimulate Pelteobagrus fulvidraco parent population gonad development；
Parent population fortification：Weigh fish meal 40-50 parts, soybean cake 30-40 parts, fish oil 40-50 parts, fish protein hydrolyzate 20-30
Part, split kettle algae 10-15 parts, chlorella 10-15 parts, shrimp shell meal 2.5-3 parts, brewer's yeast 2-2.5 parts, dextrin 12-15 parts, α-shallow lake
Powder 14-18 parts, calcium dihydrogen phosphate 2.0-3.0 parts, active peptides 0.2-0.3 parts, vitamin A 0.2-0.3 parts, vitamin D 0.2-
0.3 part, Choline Chloride 0.5-0.7 parts, Eucommia Leaf Powder 0.2-0.3 parts, dandelion extract 0.1-0.2 parts, sodium benzoate 0.1-
0.2 part well mixed be used as parent population nutrient fodder, and nursing 2-3 times daily in 2-3 months, is had enough by parent population and be defined before raising；Raising
During irregularly to be washed by water, high temperature season starts aerator in good time；To before hastening parturition 20-30 days, the parent that will reach maturity
Fish, which has caught, puts storage pond, holding pond into, and male and female are separately raised, and reduces stimulation by running water；The amino acid sequence of active peptides is：
SCASRCKSRCRARRCGYYVSVFVFYRGRCYCRCLRC；Active peptides can stimulate Pelteobagrus fulvidraco pituitary gonadotrophin secretion
Cell is developed and enters secretory activity ahead of time, is promoted the differentiation of sexual gland, is promoted the development of sexual organ；In the parent fish rearing stage, strengthen
The supplement of foreign aid's nutrition, strengthen in bait containing protein, amino acid, fat, vitamin necessary to yellow catfish growing development
Deng, and it is aided with phagostimulant, antioxidant, Chinese herbal medicine etc., the nutritional requirement needed for parent population growth and development can be met, promoted yellow
Forehead fish parent fish grows and gonad development；
Parent population is hastened parturition：Hastened parturition using the mixing of a variety of oxytocic hormones, raun per kilogram of body weight injection carp body 1-3mg adds DOM 1-
3mg, which adds, promotees human chorionic gonadtropin 10-15mg, and milter dosage is 0.5-0.7 times of raun, is divided to two pins to inject, before injection first
Row anesthesia, is injected, inserting needle 3-4mm, needle gage 10-12 hours in dorsal fin base portion soft tissue, two pins dislocation injection, in water temperature 24
Effect time is l5-22 hours at DEG C -28 DEG C, male and female in proportion 1 after injection：2-3 combos are put into spawning pond, and pay attention to adjusting water
Flow or start aerator and cause local miniflow water；A variety of hormone mixing are hastened parturition, the pin of intermittent infusion two is advantageous to Pelteobagrus fulvidraco ovulation row
Essence, the quality of smart ovum is improved, and the health of parent population will not be damaged again, prepared to lay eggs with hatching；
Spawning and collection：After parent population injects the first pin, depth of water 30-40cm spawning pond is put into, after the injection of the second pin, by the depth of water
50-60cm is adjusted, process of entirely hastening parturition keeps miniflow water, does fish nest with brown piece or bionic aquatic weed, one layer of sieve is put below fish nest
Thin,tough silk, fixed with the smooth stone in surface, brick, calculate Effect time, Pelteobagrus fulvidraco is laid eggs at night as far as possible, if can not night
Between lay eggs, it is necessary to carry out sunshade measure, it is ensured that spawning effect, after spawning terminates, take out and be stained with the fish nest of embryonated egg, and collect and dissipate
Fall the embryonated egg on bolting silk, while catch the parent population also pool；Preferable with bionic aquatic weed collection embryonated egg effect, it is convenient to collect, miscellaneous
Matter content is few, and rate of fertilization is high；
Hatching：After embryonated egg is sterile-processed, hatchery is put into, pays attention to placing uniform, 3-4 oxygenation of placement in each hatchery
Pump continues oxygenation, in order to avoid cause to press ovum phenomenon, can incubation of membrane through 60-75 hours when keeping 22-28 DEG C of water temperature；Whole hatching
Process is uninterruptedly inflated, and causes miniflow water, ensures abundant dissolved oxygen demand, be may advantageously facilitate hatching, often to be examined in hatching process
Look into, check water quality streamflow regime and embryonated egg health status；
Fry enriched nutritive：The advanced fry of yellow catfish body children just hatched is tender, carries yolk bag during seedling membrane, is not necessary to feed,
After yolk bag disappears, enriched nutritive is carried out with the artemia after fortification daily, whole enriched nutritive process is uninterruptedly inflated, made
Into miniflow water, ensure abundant dissolved oxygen demand；The reinforcing bait of artemia is：Split kettle algae 10-15 parts, chlorella 10-15 parts, lecithin
Liposome 20-25 parts, fish oil 20-25 parts, fish protein hydrolyzate 28-33 parts, urea 20-23 parts, ammonium nitrate 18-20 parts, phosphoric acid
Calcium 15-20 parts, citrus powder 2-4 parts, decomposed manure 60-80 parts, which are well mixed, is ground into 10-45 submicron size particles, halogen
Worm can be fed to fry through strengthening 8-10 hours, physical strong when fry is grown to 4-6cm, moved about flexible, you can pond-inputting is raised
Support, fish body is carried out disinfection with 2-3% saline solution before fry pond-inputting；Make polyunsaturated fatty acid in the artemia body after reinforcing
Content be improved, be suitable as the open-mouthed bait of seedling, the rearing condition be advantageous to improve the Pelteobagrus fulvidraco young immunity energy
Power, stress ability, antibacterial resistance against diseases, improve its survival rate and growth rate.
Compared with prior art, the advantage of the invention is that：1）In the parent fish rearing stage, strengthen the supplement of foreign aid's nutrition, by force
Change containing protein, amino acid, fat, vitamin etc. necessary to yellow catfish growing development in bait, and be aided with phagostimulant, resist
Oxidant, Chinese herbal medicine etc., the nutritional requirement needed for parent population growth and development can be met, promote the growth of Pelteobagrus fulvidraco parent population and sexual gland
Development；2）Active peptides can stimulate Pelteobagrus fulvidraco pituitary gonadotropin cell to develop ahead of time and enter secretory activity, promote
Enter the differentiation of sexual gland, promote the development of sexual organ；3）Measure containing for polyunsaturated fatty acid in the artemia body after reinforcing
To raising, the open-mouthed bait of seedling is suitable as, the rearing condition is advantageous to improve the immunocompetence of the Pelteobagrus fulvidraco young, stress energy
Power, antibacterial resistance against diseases, improve its survival rate and growth rate.
Embodiment
The present invention program is described further below by embodiment：
Embodiment 1：
A kind of artificial culturing method of advanced fry of yellow catfish, comprises the following steps：
1）The water quality in Pelteobagrus fulvidraco parent fish rearing pond will keep clear fresh pollution-free, and the dissolved oxygen amount of water is maintained at 8mg/L, and pH value is
8.5, in preparation hasten parturition first 1 month, every 7 will wash by water 1 time everyday, and the time washed by water every time is 50 minutes, during bath, Ke Yili
Punching note is carried out with the water in former pond, parent population selection requires male 350g, female individuals 150g；Bath makes to form water in culture pond
Stream, can stimulate Pelteobagrus fulvidraco parent population gonad development；2）Parent population nutrient fodder is prepared, is fed 2 times daily within 2 months before raising, with parent
Fish, which has enough, to be defined；Irregularly to be washed by water in feeding process, high temperature season starts aerator in good time；, will to hastening parturition first 20 days
The parent population to reach maturity, which has caught, puts storage pond, holding pond into, and male and female are separately raised, and reduces stimulation by running water；3）Mixed using a variety of oxytocic hormones
Hasten parturition, raun per kilogram of body weight injection carp body 1mg adds DOM 1mg to add rush human chorionic gonadtropin 10mg, and milter dosage is
0.5 times of raun, it is divided to two pins to inject, anaesthetizes, injected in dorsal fin base portion soft tissue, inserting needle 3mm, needle gage 10 in advance before injection
Hour, the dislocation injection of two pins, in 24 DEG C DEG C of water temperature, Effect time is l5 hours, male and female in proportion 1 after injection：2 combos are put into
Spawning pond, and pay attention to adjusting current or starting aerator causing local miniflow water；4）After parent population injects the first pin, the depth of water is put into
40cm spawning pond, after the injection of the second pin, 60cm is arrived into depth of water regulation, process of entirely hastening parturition keeps miniflow water；5）Embryonated egg
After sterile-processed, hatchery is put into, pays attention to placing uniform, the lasting oxygenation of 3 oxygen increasing pumps of placement, Yi Mianzao in each hatchery
, can incubation of membrane through 60 hours when keeping 22 DEG C of water temperature into pressure ovum phenomenon；Whole hatching process is uninterruptedly inflated, and causes miniflow
Water, ensure abundant dissolved oxygen demand, may advantageously facilitate hatching, running check is wanted in hatching process, check water quality streamflow regime and by
Smart ovum health status；6）The advanced fry of yellow catfish body children just hatched is tender, carries yolk bag during seedling membrane, is not necessary to feed, ovum
After yellow capsule disappears, enriched nutritive is carried out with the artemia after fortification daily, whole enriched nutritive process is uninterruptedly inflated, caused
Miniflow water, ensure abundant dissolved oxygen demand；The reinforcing bait of artemia is：Split 10 parts of kettle algae, 10 parts of chlorella, lecithin liposome 20
Part, 20 parts of fish oil, 28 parts of fish protein hydrolyzate, 20 parts of urea, 18 parts of ammonium nitrate, 20 parts of calcium phosphate, 2 parts of citrus powder, decomposed have
60 parts of machine fertilizer is well mixed to be ground into 10 submicron size particles, and artemia can be fed to fry through strengthening 8 hours, treat fry
It is physical strong when length is to 4cm, move about flexible, you can pond-inputting is raised, and fish body is carried out disinfection with 2% saline solution before fry pond-inputting.
Embodiment 2：
A kind of artificial culturing method of advanced fry of yellow catfish, comprises the following steps：
1) parent fish rearing：The water quality in Pelteobagrus fulvidraco parent fish rearing pond will keep clear fresh pollution-free, and the dissolved oxygen amount of water is maintained at 8mg/
L, pH value 68.5, first 2 months to be hastened parturition in preparation, to wash by water 1 time within every 10 days, the time washed by water every time is 80 minutes, bath
When, punching note can be carried out using the water in former pond, parent population selection requires male 400g, female individuals 200g；Bath makes cultivation
Current are formed in pond, Pelteobagrus fulvidraco parent population gonad development can be stimulated；
2) parent population fortification：Weigh 50 parts of fish meal, 40 parts of soybean cake, 50 parts of fish oil, 30 parts of fish protein hydrolyzate, split kettle algae 15
Part, 15 parts of chlorella, 3 parts of shrimp shell meal, 2.5 parts of brewer's yeast, 15 parts of dextrin, 18 parts of alphalise starch, 3.0 parts of calcium dihydrogen phosphate, work
Property 0.3 part of polypeptide, 0.3 part of vitamin A, 0.3 part of vitamin D, 0.7 part of Choline Chloride, 0.3 part of Eucommia Leaf Powder, dandelion extraction
0.2 part of thing, 0.2 part of sodium benzoate are well mixed and are used as parent population nutrient fodder, feed 3 times within 3 months before raising, are eaten with parent population daily
It is full to be defined；Irregularly to be washed by water in feeding process, high temperature season starts aerator in good time；To hastening parturition first 30 days, will develop
Ripe parent population, which has caught, puts storage pond, holding pond into, and male and female are separately raised, and reduces stimulation by running water；
3) parent population is hastened parturition：Raun per kilogram of body weight injection carp body 3mg adds DOM 3mg to add rush human chorionic gonadtropin 15mg,
Milter dosage is 0.7 times of raun, is divided to two pins to inject, anaesthetizes, injected in dorsal fin base portion soft tissue, inserting needle in advance before injection
4mm, needle gage 12 hours, the dislocation injection of two pins, in 28 DEG C of water temperature, Effect time is 22 hours, male and female in proportion 1 after injection：3
Combo is put into spawning pond, and pays attention to adjusting current or starting aerator causing local miniflow water；A variety of hormone mixing are hastened parturition, interval
Inject two pins and be advantageous to Pelteobagrus fulvidraco ovulation row's essence, improve the quality of smart ovum, and the health of parent population will not be damaged again, for spawning with
Hatching is prepared；
4) spawning and collection：After parent population injects the first pin, depth of water 40cm spawning pond is put into, after the injection of the second pin, by the depth of water
60cm is adjusted, process of entirely hastening parturition keeps miniflow water, and fish nest is done with brown piece or bionic aquatic weed;Embryonated egg is collected with bionic aquatic weed
Effect is preferable, and it is convenient to collect, and impurity content is few, and rate of fertilization is high；
5) hatch：After embryonated egg is sterile-processed, hatchery is put into, pays attention to placing uniform, 4 oxygenation of placement in each hatchery
Pump continues oxygenation, in order to avoid cause to press ovum phenomenon, can incubation of membrane through 75 hours when keeping 28 DEG C of water temperature；Whole hatching process is not
Interruption inflation, causes miniflow water, ensures abundant dissolved oxygen demand, may advantageously facilitate hatching, want running check in hatching process, check
Water quality streamflow regime and embryonated egg health status；
6) fry enriched nutritive：The advanced fry of yellow catfish body children just hatched is tender, carries yolk bag during seedling membrane, is not necessary to throw
Hello, after yolk bag disappears, enriched nutritive is carried out with the artemia after fortification daily, whole enriched nutritive process is uninterruptedly filled
Gas, miniflow water is caused, ensure abundant dissolved oxygen demand；Artemia can be fed to fry through strengthening 10 hours, treat that fry is grown to 6cm
When, it is physical strong, move about flexible, you can pond-inputting is raised, and fish body is carried out disinfection with 3% saline solution before fry pond-inputting；Make through too strong
The content of polyunsaturated fatty acid is improved in artemia body after change, is suitable as the open-mouthed bait of seedling, the rearing condition
Be advantageous to improve immunocompetence, stress ability, the antibacterial resistance against diseases of the Pelteobagrus fulvidraco young, improve its survival rate and speed of growing
Degree.
Embodiment 3：
A kind of artificial culturing method of advanced fry of yellow catfish, including：Parent fish rearing, parent population fortification, parent population are hastened parturition, laid eggs with receiving
Collection, hatching, fry enriched nutritive, specifically include following steps：
Parent fish rearing：The water quality in Pelteobagrus fulvidraco parent fish rearing pond will keep clear fresh pollution-free, and the dissolved oxygen amount of water is maintained at 6mg/L,
PH value is 7, in preparation hasten parturition first 45 days, to be washed by water 1 time within every 8 days, and the time washed by water every time is 60 minutes, can be with during bath
Punching note is carried out using the water in former pond, parent population selection requires male 400g, female individuals 200g；Bath makes to be formed in culture pond
Current, Pelteobagrus fulvidraco parent population gonad development can be stimulated；
Parent population fortification：Weigh 50 parts of fish meal, 40 parts of soybean cake, 50 parts of fish oil, 25 parts of fish protein hydrolyzate, split kettle algae 10
Part, 12 parts of chlorella, 2.5 parts of shrimp shell meal, 2 parts of brewer's yeast, 15 parts of dextrin, 16 parts of alphalise starch, 2.8 parts of calcium dihydrogen phosphate, work
Property 0.2 part of polypeptide, 0.3 part of vitamin A, 0.3 part of vitamin D, 0.7 part of Choline Chloride, 0.3 part of Eucommia Leaf Powder, dandelion extraction
0.2 part of thing, 0.2 part of sodium benzoate are well mixed and are used as parent population nutrient fodder, feed 3 times within 2 months before raising, are eaten with parent population daily
It is full to be defined；Irregularly to be washed by water in feeding process, high temperature season starts aerator in good time；To hastening parturition first 30 days, will develop
Ripe parent population, which has caught, puts storage pond, holding pond into, and male and female are separately raised, and reduces stimulation by running water；The amino acid sequence of active peptides is：
SCASRCKSRCRARRCGYYVSVFVFYRGRCYCRCLRC；Active peptides can stimulate Pelteobagrus fulvidraco pituitary gonadotrophin secretion
Cell is developed and enters secretory activity ahead of time, is promoted the differentiation of sexual gland, is promoted the development of sexual organ；In the parent fish rearing stage, strengthen
The supplement of foreign aid's nutrition, strengthen in bait containing protein, amino acid, fat, vitamin necessary to yellow catfish growing development
Deng, and it is aided with phagostimulant, antioxidant, Chinese herbal medicine etc., the nutritional requirement needed for parent population growth and development can be met, promoted yellow
Forehead fish parent fish grows and gonad development；
Parent population is hastened parturition：Hastened parturition using the mixing of a variety of oxytocic hormones, raun per kilogram of body weight injection carp body 3mg adds DOM 2mg to add
Promote human chorionic gonadtropin 12mg, milter dosage is 0.6 times of raun, is divided to two pins to inject, is anaesthetized in advance before injection, in dorsal fin
Base portion soft tissue is injected, inserting needle 4mm, needle gage 12 hours, and the dislocation injection of two pins, in 26 DEG C of water temperature, Effect time is 20 small
When, male and female in proportion 1 after injection：3 combos are put into spawning pond, and pay attention to adjusting current or starting aerator causing local miniflow
Water；A variety of hormone mixing are hastened parturition, the pin of intermittent infusion two is advantageous to Pelteobagrus fulvidraco ovulation row's essence, improve the quality of smart ovum, and again not
The health of parent population can be damaged, is prepared to lay eggs with hatching；
Spawning and collection：After parent population injects the first pin, depth of water 40cm spawning pond is put into, after the injection of the second pin, the depth of water is adjusted
60cm is saved, process of entirely hastening parturition keeps miniflow water, does fish nest with brown piece or bionic aquatic weed, one layer of bolting silk is put below fish nest, uses
The smooth stone in surface, brick are fixed, and calculate Effect time, Pelteobagrus fulvidraco is laid eggs at night as far as possible, if can not produce at night
Ovum, it is necessary to carry out sunshade measure, it is ensured that spawning effect, after spawning terminates, take out the fish nest for being stained with embryonated egg, and collect and be scattered in
Embryonated egg on bolting silk, while caught the parent population also pool；Preferable with bionic aquatic weed collection embryonated egg effect, it is convenient to collect, and impurity contains
Amount is few, and rate of fertilization is high；
Hatching：After embryonated egg is sterile-processed, hatchery is put into, pays attention to placing uniform, 4 oxygen increasing pumps of placement in each hatchery
Continue oxygenation, in order to avoid cause to press ovum phenomenon, can incubation of membrane through 60 hours when keeping 26 DEG C of water temperature；Whole hatching process not between
Disconnected inflation, causes miniflow water, ensures abundant dissolved oxygen demand, may advantageously facilitate hatching, want running check in hatching process, check water
Matter streamflow regime and embryonated egg health status；
Fry enriched nutritive：The advanced fry of yellow catfish body children just hatched is tender, carries yolk bag during seedling membrane, is not necessary to feed,
After yolk bag disappears, enriched nutritive is carried out with the artemia after fortification daily, whole enriched nutritive process is uninterruptedly inflated, made
Into miniflow water, ensure abundant dissolved oxygen demand；The reinforcing bait of artemia is：Split 12 parts of kettle algae, 12 parts of chlorella, lecithin liposome
24 parts, it is 24 parts of fish oil, 30 parts of fish protein hydrolyzate, 20 parts of urea, 20 parts of ammonium nitrate, 20 parts of calcium phosphate, 3 parts of citrus powder, decomposed
80 parts of organic fertilizer is well mixed to be ground into 30 submicron size particles, and artemia can feed to fry through strengthening 10 hours, treat
It is physical strong when fry is grown to 6cm, move about flexible, you can pond-inputting is raised, and fish body is disappeared with 3% saline solution before fry pond-inputting
Poison；The content of polyunsaturated fatty acid in the artemia body after reinforcing is improved, is suitable as the open-mouthed bait of seedling,
The rearing condition be advantageous to improve the Pelteobagrus fulvidraco young immunocompetence, stress ability, antibacterial resistance against diseases, improve its survival rate with
Growth rate.
Routine operation in the operating procedure of the present invention is well known to those skilled in the art, herein without repeating.
Technical scheme is described in detail embodiment described above, it should be understood that it is described above only
For the specific embodiment of the present invention, it is not intended to limit the invention, all any modifications made in the spirit of the present invention,
Supplement or similar fashion replacement etc., should be included in the scope of the protection.
SEQUENCE LISTING
<110>Lanxi City Pu Run Si aquaculture technologies Co., Ltd
<120>A kind of artificial culturing method of advanced fry of yellow catfish
<130> 2
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 36
<212> PRT
<213>It is artificial synthesized
<400> 1
Ser Cys Ala Ser Arg Cys Lys Ser Arg Cys Arg Ala Arg Arg Cys Gly
1 5 10 15
Tyr Tyr Val Ser Val Phe Val Phe Tyr Arg Gly Arg Cys Tyr Cys Arg
20 25 30
Cys Leu Arg Cys
35
Claims (8)
1. a kind of artificial culturing method of advanced fry of yellow catfish, including：Parent fish rearing, parent population fortification, parent population hasten parturition, lay eggs with
Collect, hatch, fry enriched nutritive, it is characterised in that add in described parent population fortification step in the feed of fortification
A kind of active peptides are entered.
A kind of 2. artificial culturing method of advanced fry of yellow catfish according to claim 1, it is characterised in that：Described parent population training
Educating step is：The water quality in Pelteobagrus fulvidraco parent fish rearing pond will keep clear fresh pollution-free, and the dissolved oxygen amount of water is maintained at 3.5-8mg/L,
PH value is 6.5-8.5, in preparation hasten parturition first -2 months 1 month, to be washed by water 1 time within every -10 days 7 days, and the time washed by water every time is
50-80 minutes, during bath, punching note can be carried out using the water in former pond, parent population selection requires male 350-400g, female
Body 150-200g.
A kind of 3. artificial culturing method of advanced fry of yellow catfish according to claim 1, it is characterised in that：Described parent population battalion
Supporting reinforcing step is：Weigh fish meal 40-50 parts, soybean cake 30-40 parts, fish oil 40-50 parts, fish protein hydrolyzate 20-30 parts, split
Kettle algae 10-15 parts, chlorella 10-15 parts, shrimp shell meal 2.5-3 parts, brewer's yeast 2-2.5 parts, dextrin 12-15 parts, alphalise starch 14-
18 parts, calcium dihydrogen phosphate 2.0-3.0 parts, active peptides 0.2-0.3 parts, vitamin A 0.2-0.3 parts, vitamin D 0.2-0.3 parts,
Choline Chloride 0.5-0.7 parts, Eucommia Leaf Powder 0.2-0.3 parts, dandelion extract 0.1-0.2 parts, sodium benzoate 0.1-0.2 parts are mixed
Conjunction is uniformly used as parent population nutrient fodder, feeds 2-3 times daily within 2-3 months before raising, is had enough and be defined by parent population；Will in feeding process
Irregularly washed by water, high temperature season starts aerator in good time；To before hastening parturition 20-30 days, the parent population to reach maturity is caught and put
Enter storage pond, holding pond, male and female are separately raised, and reduce stimulation by running water.
A kind of 4. artificial culturing method of advanced fry of yellow catfish according to claim 1, it is characterised in that：Described parent population battalion
Supporting the amino acid sequence of active peptides strengthened in step is：SCASRCKSRCRARRCGYYVSVFVFYRGRCYCRCLRC.
A kind of 5. artificial culturing method of advanced fry of yellow catfish according to claim 1, it is characterised in that：Described parent population is urged
Producing step is：Hastened parturition using the mixing of a variety of oxytocic hormones, raun per kilogram of body weight injection carp body 1-3mg adds DOM 1-3mg to add
Promote human chorionic gonadtropin 10-15mg, milter dosage is 0.5-0.7 times of raun, is divided to two pins to inject, numb in advance before injection
It is liquor-saturated, injected in dorsal fin base portion soft tissue, inserting needle 3-4mm, needle gage 10-12 hours, the dislocation injection of two pins, 24 DEG C of water temperature-
Effect time is l5-22 hours at 28 DEG C, male and female in proportion 1 after injection：2-3 combos are put into spawning pond, and pay attention to adjusting current
Or start aerator and cause local miniflow water.
A kind of 6. artificial culturing method of advanced fry of yellow catfish according to claim 1, it is characterised in that：Described spawning with
Collection step is：After parent population injects the first pin, depth of water 30-40cm spawning pond is put into, after the injection of the second pin, the depth of water is adjusted
To 50-60cm, process of entirely hastening parturition keeps miniflow water, does fish nest with brown piece or bionic aquatic weed, one layer of bolting silk is put below fish nest, uses
The smooth stone in surface, brick are fixed, and calculate Effect time, Pelteobagrus fulvidraco is laid eggs at night as far as possible, if can not produce at night
Ovum, it is necessary to carry out sunshade measure, it is ensured that spawning effect, after spawning terminates, take out the fish nest for being stained with embryonated egg, and collect and be scattered in
Embryonated egg on bolting silk, while caught the parent population also pool.
A kind of 7. artificial culturing method of advanced fry of yellow catfish according to claim 1, it is characterised in that：Described hatching step
Suddenly it is：After embryonated egg is sterile-processed, hatchery is put into, pays attention to placing uniformly, 3-4 oxygen increasing pump is placed in each hatchery and is held
Continuous oxygenation,, can incubation of membrane through 60-75 hours when keeping 22-28 DEG C of water temperature in order to avoid cause to press ovum phenomenon.
A kind of 8. artificial culturing method of advanced fry of yellow catfish according to claim 1, it is characterised in that：Described fry is strong
Changing nutrition step is：The advanced fry of yellow catfish body children just hatched is tender, carries yolk bag during seedling membrane, is not necessary to feed, yolk
After capsule disappears, enriched nutritive is carried out with the artemia after fortification daily, whole enriched nutritive process is uninterruptedly inflated, caused micro-
Flowing water, ensure abundant dissolved oxygen demand；The reinforcing bait of artemia is：Split kettle algae 10-15 parts, chlorella 10-15 parts, lecithin lipid
Body 20-25 parts, fish oil 20-25 parts, fish protein hydrolyzate 28-33 parts, urea 20-23 parts, ammonium nitrate 18-20 parts, calcium phosphate
15-20 parts, citrus powder 2-4 parts, decomposed manure 60-80 parts, which are well mixed, is ground into 10-45 submicron size particles, artemia
It can be fed to fry through strengthening 8-10 hours, it is physical strong when fry is grown to 4-6cm, move about flexible, you can pond-inputting is raised
Support, fish body is carried out disinfection with 2-3% saline solution before fry pond-inputting.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108041349A (en) * | 2017-12-26 | 2018-05-18 | 重庆医学检验试剂研究所 | A kind of pharmaceutical composition for promoting aquatic livestock growth and its reagent and method |
| CN110622887A (en) * | 2019-08-14 | 2019-12-31 | 天津蕴华农业科技发展有限公司 | High-yield cultivation method for pelteobagrus fulvidraco |
| CN111296331A (en) * | 2020-03-09 | 2020-06-19 | 广西壮族自治区水产科学研究院 | Artificial breeding and seedling raising method for pelteobagrus fulvidraco |
| CN112154946A (en) * | 2020-11-05 | 2021-01-01 | 华中农业大学 | Method for cultivating indoor controllable inactivated bait fish with initial fish fries |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1383733A (en) * | 2001-04-30 | 2002-12-11 | 陆晓民 | Fish protein polypeptide aquatic animal feed |
| CN104054597A (en) * | 2014-03-17 | 2014-09-24 | 浙江省海洋水产研究所 | Nibea albiflora fry artificial mixed feed domestication method |
| CN104996833A (en) * | 2015-08-12 | 2015-10-28 | 四川国凤中科生物科技有限公司 | Pelteobagrus fulvidraco feed |
| CN105166455A (en) * | 2015-08-10 | 2015-12-23 | 四川斯特佳饲料有限公司 | New-type environmentally protective, nutritional, and high-efficient pelteobagrus fulvidraco compound feed |
| CN106551186A (en) * | 2016-11-16 | 2017-04-05 | 南宁学院 | A kind of new parr of yellow catfish feedstuff |
-
2017
- 2017-07-31 CN CN201710643603.7A patent/CN107494344A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1383733A (en) * | 2001-04-30 | 2002-12-11 | 陆晓民 | Fish protein polypeptide aquatic animal feed |
| CN104054597A (en) * | 2014-03-17 | 2014-09-24 | 浙江省海洋水产研究所 | Nibea albiflora fry artificial mixed feed domestication method |
| CN105166455A (en) * | 2015-08-10 | 2015-12-23 | 四川斯特佳饲料有限公司 | New-type environmentally protective, nutritional, and high-efficient pelteobagrus fulvidraco compound feed |
| CN104996833A (en) * | 2015-08-12 | 2015-10-28 | 四川国凤中科生物科技有限公司 | Pelteobagrus fulvidraco feed |
| CN106551186A (en) * | 2016-11-16 | 2017-04-05 | 南宁学院 | A kind of new parr of yellow catfish feedstuff |
Non-Patent Citations (2)
| Title |
|---|
| 祖国掌 主编: "《科学养鱼大全（第二版）》", 31 March 2015, 安徽科学技术出版社 * |
| 陈杰 等: "《无鳞鱼养殖新技术》", 30 June 2008, 中原农民出版社 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108041349A (en) * | 2017-12-26 | 2018-05-18 | 重庆医学检验试剂研究所 | A kind of pharmaceutical composition for promoting aquatic livestock growth and its reagent and method |
| CN110622887A (en) * | 2019-08-14 | 2019-12-31 | 天津蕴华农业科技发展有限公司 | High-yield cultivation method for pelteobagrus fulvidraco |
| CN111296331A (en) * | 2020-03-09 | 2020-06-19 | 广西壮族自治区水产科学研究院 | Artificial breeding and seedling raising method for pelteobagrus fulvidraco |
| CN111296331B (en) * | 2020-03-09 | 2022-05-13 | 广西壮族自治区水产科学研究院 | Artificial breeding and seedling raising method for pelteobagrus fulvidraco |
| CN112154946A (en) * | 2020-11-05 | 2021-01-01 | 华中农业大学 | Method for cultivating indoor controllable inactivated bait fish with initial fish fries |
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