CN111296331B - Artificial breeding and seedling raising method for pelteobagrus fulvidraco - Google Patents
Artificial breeding and seedling raising method for pelteobagrus fulvidraco Download PDFInfo
- Publication number
- CN111296331B CN111296331B CN202010155497.XA CN202010155497A CN111296331B CN 111296331 B CN111296331 B CN 111296331B CN 202010155497 A CN202010155497 A CN 202010155497A CN 111296331 B CN111296331 B CN 111296331B
- Authority
- CN
- China
- Prior art keywords
- pelteobagrus fulvidraco
- water
- parts
- breeding
- feed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 241000376029 Tachysurus fulvidraco Species 0.000 title claims abstract description 111
- 238000009395 breeding Methods 0.000 title claims abstract description 73
- 230000001488 breeding effect Effects 0.000 title claims abstract description 73
- 238000000034 method Methods 0.000 title claims abstract description 28
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 115
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 45
- 235000013601 eggs Nutrition 0.000 claims abstract description 37
- 241000251468 Actinopterygii Species 0.000 claims abstract description 30
- XNOPRXBHLZRZKH-UHFFFAOYSA-N Oxytocin Natural products N1C(=O)C(N)CSSCC(C(=O)N2C(CCC2)C(=O)NC(CC(C)C)C(=O)NCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(CCC(N)=O)NC(=O)C(C(C)CC)NC(=O)C1CC1=CC=C(O)C=C1 XNOPRXBHLZRZKH-UHFFFAOYSA-N 0.000 claims abstract description 26
- 101800000989 Oxytocin Proteins 0.000 claims abstract description 26
- 102100031951 Oxytocin-neurophysin 1 Human genes 0.000 claims abstract description 26
- XNOPRXBHLZRZKH-DSZYJQQASA-N oxytocin Chemical compound C([C@H]1C(=O)N[C@H](C(N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CSSC[C@H](N)C(=O)N1)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CC(C)C)C(=O)NCC(N)=O)=O)[C@@H](C)CC)C1=CC=C(O)C=C1 XNOPRXBHLZRZKH-DSZYJQQASA-N 0.000 claims abstract description 26
- 229960001723 oxytocin Drugs 0.000 claims abstract description 26
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 11
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 11
- 239000001301 oxygen Substances 0.000 claims abstract description 11
- 238000005086 pumping Methods 0.000 claims abstract description 11
- 244000017020 Ipomoea batatas Species 0.000 claims description 34
- 235000002678 Ipomoea batatas Nutrition 0.000 claims description 34
- 238000002156 mixing Methods 0.000 claims description 30
- 240000008042 Zea mays Species 0.000 claims description 23
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 23
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 23
- 235000005822 corn Nutrition 0.000 claims description 23
- 238000012216 screening Methods 0.000 claims description 21
- 230000016087 ovulation Effects 0.000 claims description 20
- 230000001737 promoting effect Effects 0.000 claims description 20
- 241000196324 Embryophyta Species 0.000 claims description 19
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 16
- MBLBDJOUHNCFQT-LXGUWJNJSA-N aldehydo-N-acetyl-D-glucosamine Chemical compound CC(=O)N[C@@H](C=O)[C@@H](O)[C@H](O)[C@H](O)CO MBLBDJOUHNCFQT-LXGUWJNJSA-N 0.000 claims description 16
- 235000021015 bananas Nutrition 0.000 claims description 16
- 239000008103 glucose Substances 0.000 claims description 16
- 239000000843 powder Substances 0.000 claims description 16
- 238000000855 fermentation Methods 0.000 claims description 15
- 230000004151 fermentation Effects 0.000 claims description 15
- 230000004720 fertilization Effects 0.000 claims description 14
- 230000012447 hatching Effects 0.000 claims description 14
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 12
- 239000007788 liquid Substances 0.000 claims description 12
- 240000007817 Olea europaea Species 0.000 claims description 9
- 208000003643 Callosities Diseases 0.000 claims description 8
- 206010020649 Hyperkeratosis Diseases 0.000 claims description 8
- 238000010411 cooking Methods 0.000 claims description 8
- 238000005360 mashing Methods 0.000 claims description 8
- 239000012528 membrane Substances 0.000 claims description 8
- 230000032696 parturition Effects 0.000 claims description 8
- 102000011022 Chorionic Gonadotropin Human genes 0.000 claims description 7
- 108010062540 Chorionic Gonadotropin Proteins 0.000 claims description 7
- 229940015047 chorionic gonadotropin Drugs 0.000 claims description 7
- 235000013312 flour Nutrition 0.000 claims description 7
- 239000003488 releasing hormone Substances 0.000 claims description 7
- 210000000582 semen Anatomy 0.000 claims description 7
- 238000007865 diluting Methods 0.000 claims description 6
- 238000011534 incubation Methods 0.000 claims description 3
- 240000008564 Boehmeria nivea Species 0.000 claims description 2
- 240000002853 Nelumbo nucifera Species 0.000 claims description 2
- 235000006508 Nelumbo nucifera Nutrition 0.000 claims description 2
- 235000006510 Nelumbo pentapetala Nutrition 0.000 claims description 2
- 238000000605 extraction Methods 0.000 claims description 2
- 240000008790 Musa x paradisiaca Species 0.000 claims 2
- 230000006698 induction Effects 0.000 abstract description 2
- 244000291473 Musa acuminata Species 0.000 description 14
- 230000000052 comparative effect Effects 0.000 description 13
- 238000003756 stirring Methods 0.000 description 6
- 238000007605 air drying Methods 0.000 description 5
- 230000001678 irradiating effect Effects 0.000 description 5
- 238000010298 pulverizing process Methods 0.000 description 5
- 230000004083 survival effect Effects 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 3
- 235000013339 cereals Nutrition 0.000 description 3
- 230000017448 oviposition Effects 0.000 description 3
- 102000002322 Egg Proteins Human genes 0.000 description 2
- 108010000912 Egg Proteins Proteins 0.000 description 2
- 241000252496 Siluriformes Species 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 210000004681 ovum Anatomy 0.000 description 2
- 241000195493 Cryptophyta Species 0.000 description 1
- 241001314343 Pseudobagrus Species 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 230000001771 impaired effect Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/66—Treatment of water, waste water, or sewage by neutralisation; pH adjustment
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Organic Chemistry (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses a method for artificial propagation and breeding of pelteobagrus fulvidraco, which comprises the steps of selecting mature pelteobagrus fulvidraco, and feeding female and male pelteobagrus fulvidraco separately; adding oxytocin into feed for feeding for a period of time, simultaneously increasing white light irradiation, and adding ovulation-promoting agent in later stage; the method comprises the following steps of (1) feeding female fishes and male fishes in a mixed manner, dividing the female fishes and the male fishes into two parts by using a fishing net, dividing all pelteobagrus fulvidraco into two parts, feeding the pelteobagrus fulvidraco at one side of a water inlet of a water pool, adding a spawning agent into water, and keeping running water flowing; keeping the water temperature and adjusting the pH value of the water, and pumping oxygen into the water pool to be fertilized and incubated by the fish eggs; the breeding range of the hatched fries is properly widened, then the fry breeding feed is dissolved in water and is guided into the water for breeding, a proper amount of white light irradiation is provided, and the fries with different volumes are screened and separately bred after 10 to 20 days. The method can rapidly carry out artificial breeding operation and seedling raising, can carry out artificial spawning induction on the pelteobagrus fulvidraco in a more natural state, and is simple and convenient to operate and good in breeding effect.
Description
Technical Field
The invention relates to the technical field of fish fry breeding, in particular to an artificial breeding and fry breeding method for pelteobagrus fulvidraco.
Background
The yellow catfish belongs to the catfishes, the pseudobagaceae and the pseudobagrus, is a high-quality rare economic fish in a fresh water area, is deeply favored by people due to delicious meat quality, but the fishing amount of rivers cannot meet the market demand easily; in recent years, artificial breeding of pelteobagrus fulvidraco has been developed in many places throughout the country, the breeding scale is increased year by year, and the breeding yield of pelteobagrus fulvidraco in China exceeds 350000 tons in 2015. Fry breeding of catfishes such as pelteobagrus fulvidraco basically comprises artificial spawning induction, repeated injection of medicaments, emasculation and sperm extraction, and hatching in a special hatching tank; the proportion of male and female in natural waters is seriously imbalanced, so that natural resources are seriously exhausted, certain hidden danger exists in edible safety, and the work efficiency is slow through the artificial injection induced spawning technology, so that a method capable of solving the problems needs to be found.
Disclosure of Invention
The invention aims to: aiming at the problems, the method for quickly carrying out artificial propagation operation and seedling culture is provided, the artificial induced spawning of the pelteobagrus fulvidraco can be carried out in a more natural state by the method, the operation is simple and convenient, and the propagation effect is good.
In order to achieve the purpose of the invention, the technical scheme adopted by the invention is as follows:
an artificial breeding and seedling raising method for pelteobagrus fulvidraco comprises the following steps:
(1) parent screening: selecting a pelteobagrus fulvidraco which is about to mature and is large in the same period, feeding the female and male pelteobagrus fulvidraco separately and feeding the same feed until the pelteobagrus fulvidraco is completely mature for later use;
(2) hastening parturition before breeding: adding oxytocin into feed for feeding for a period of time, simultaneously increasing white light irradiation, and adding ovulation-promoting agent into feed of later-stage female fish;
(3) fertilization and propagation: the method comprises the following steps of (1) feeding female pelteobagrus fulvidraco and male pelteobagrus fulvidraco in a mixed mode, then dividing a water pool into two parts by using a fishing net, dividing all pelteobagrus fulvidraco into two parts, feeding the pelteobagrus fulvidraco at one side of a water inlet of the water pool, adding a spawning agent into water, and keeping running water flowing;
(4) hatching management: after the female pelteobagrus fulvidraco and the male pelteobagrus fulvidraco lay eggs, keeping the water temperature and adjusting the pH value in the water, and pumping oxygen into the water pool to wait for fertilization and incubation of fish eggs;
(5) seedling cultivation and screening: the breeding range of the hatched fries is properly widened, then the fry breeding feed is dissolved in water and is guided into the water for breeding, a proper amount of white light irradiation is provided, and the fries with different volumes are screened and separately bred after 10 to 20 days.
Further, in the step (1), the pelteobagrus fulvidraco in the same period is bred in the postnatal period for 5-6 months, the weight of the pelteobagrus fulvidraco is 80-100g, the feed is prepared by cooking sweet potatoes, mashing and mixing the cooked sweet potatoes and bananas, adding shrimp shell powder, sweet potato stems and leaves, corn and glucose, completely crushing, mixing, airing and crushing again.
Further, in the step (1), the feed comprises 80-120 parts of sweet potatoes, 10-50 parts of bananas, 20-60 parts of shrimp shell powder, 70-100 parts of sweet potato stems and leaves, 30-40 parts of corns and 1-3 parts of glucose by weight.
In the step (2), the white light irradiation is carried out for 1-3h by using 80-120Lx white light irradiation, the oxytocin is chorionic gonadotropin, the addition amount is 0.05-0.5 percent of the mass of the feed, and the ovulation promoting agent is added into the feed for feeding after the addition of the oxytocin is stopped.
Furthermore, the ovulation promoting agent is prepared by extracting 10-12 parts of semen cuscutae, 6-8 parts of ramie root, 11-13 parts of lotus petiole and 5-7 parts of folium artemisiae argyi with alcohol, concentrating into extract, adding 0.1-1 part of luteinizing releasing hormone and 50-100 parts of corn flour, uniformly mixing, airing and crushing.
Further, in the step (3), the temperature of the water in the water tank is 25-28 ℃, the oviposition agent is a plant oviposition agent, and the fermentation liquor is diluted and poured into the water tank after the fermentation of plant leaves.
Further, the plant leaves are olive leaves.
Further, in the step (4), the water temperature is adjusted to 20-23 ℃, the pH value of the water is adjusted to 6-7, and the fertilized eggs are slowly stirred while pumping oxygen, so that the fertilized eggs are subjected to membrane rupture separation as soon as possible.
Further, in the step (5), the white light irradiation is performed for 1 to 3 hours by using white light irradiation of 50 to 80Lx, and the irradiation is continued after being extinguished for 4 to 8 hours.
In summary, due to the adoption of the technical scheme, the invention has the beneficial effects that:
1. according to the method, the special properties that the illumination can promote the increase of cell activity and promote the living division of cells are utilized, so that sufficient illumination is ensured for the female pelteobagrus fulvidraco when the egg cells are bred, and the white light can also ensure the growth of algae in water to provide food for the pelteobagrus fulvidraco;
2. according to the invention, the pelteobagrus fulvidraco is isolated at one side of the water inlet for breeding and discharging the ovum and the sperm during fertilization breeding, so that the phenomenon that the output of the fry is influenced by the miseating of the discharged ovum and sperm during feeding of the pelteobagrus fulvidraco is avoided.
3. According to the invention, the water body can be adjusted to be weakly acidic by adding the oviposition agent taking the olive leaves as the raw material, and the fermentation product in the olive leaves is beneficial to stimulating the pelteobagrus fulvidraco to oviposit as soon as possible.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention will be described in further detail below with reference to preferred embodiments. It should be noted, however, that the numerous details set forth in the description are merely for the purpose of providing the reader with a thorough understanding of one or more aspects of the present invention, which may be practiced without these specific details.
Example 1
An artificial breeding and seedling raising method for pelteobagrus fulvidraco comprises the following steps:
(1) parent screening: selecting pelteobagrus fulvidraco which is about to mature and large in the same period, feeding the female and male pelteobagrus fulvidraco separately and feeding the same feed, keeping the pelteobagrus fulvidraco fully mature for later use, breeding the pelteobagrus fulvidraco for 5-6 months after birth, keeping the weight of the pelteobagrus fulvidraco at 80-100g, cooking sweet potatoes, mashing and mixing the feed with bananas, adding shrimp shell powder, sweet potato stems and leaves, corn and glucose, fully crushing, mixing, airing and crushing again to form the feed; the feed comprises 80 parts of sweet potatoes, 10 parts of bananas, 20 parts of shrimp shell powder, 70 parts of sweet potato stems and leaves, 30 parts of corns and 1 part of glucose in parts by weight;
(2) hastening parturition before breeding: adding oxytocin into feed for feeding for a period of time, simultaneously increasing white light irradiation, and adding an ovulation promoting agent into the feed of later-stage female fish, wherein the white light irradiation is 80Lx white light irradiation for 1h, the oxytocin is chorionic gonadotropin, the addition amount of the oxytocin is 0.05 percent of the mass of the feed, and the ovulation promoting agent is added into the feed for feeding after the oxytocin addition is stopped; the ovulation promoting agent is prepared by extracting semen Cuscutae 10 parts, radix Boehmeriae 6-8 parts, petiolus Nelumbinis 11 parts, and folium Artemisiae Argyi 5 parts with ethanol, concentrating into extract, adding luteinizing releasing hormone 0.1 part, adding corn flour 50 parts, mixing, air drying, and pulverizing;
(3) fertilization and propagation: mixing and feeding female pelteobagrus fulvidraco and male pelteobagrus fulvidraco, then dividing a water pool into two parts by using a fishing net, dividing all pelteobagrus fulvidraco into one side of a water inlet of the water pool for feeding, adding a spawning agent into water, keeping running water flowing, wherein the spawning agent is a plant spawning agent, diluting a fermentation liquid after plant leaves are fermented, and pouring the fermentation liquid into the water pool, wherein the plant leaves are olive leaves;
(4) hatching management: after the female pelteobagrus fulvidraco and the male pelteobagrus fulvidraco lay eggs, keeping the water temperature to 20 ℃, adjusting the pH value of the water to 6, pumping oxygen into the water pool, and slowly stirring to ensure that fertilized eggs are separated by breaking membranes as soon as possible, and after the fish eggs are fertilized and incubated, keeping the water temperature in the water pool at 25 ℃;
(5) seedling cultivation and screening: properly widening the breeding range of the hatched fries, then dissolving the fry breeding feed in water, introducing the dissolved fry breeding feed into water for feeding, providing a proper amount of white light irradiation, irradiating for 1 hour by using white light of 50Lx, continuing irradiation after extinguishing for 4 hours, and screening the fries with different volumes after 10 days and separately breeding.
Example 2
An artificial breeding and seedling raising method for pelteobagrus fulvidraco comprises the following steps:
(1) parent screening: the method comprises the steps of selecting pelteobagrus fulvidraco which is about to mature and large in the same period, feeding female and male pelteobagrus fulvidraco separately and feeding the same feed, waiting for the pelteobagrus fulvidraco to be completely mature for later use, breeding the pelteobagrus fulvidraco for 6 months after birth, keeping the weight of the pelteobagrus fulvidraco at 100g, cooking sweet potatoes, mashing and mixing the cooked feed with bananas, adding shrimp shell powder, sweet potato stem leaves, corn and glucose, completely crushing, mixing, airing and crushing again to form the feed; the feed comprises the following ingredients, by weight, 120 parts of sweet potatoes, 50 parts of bananas, 60 parts of shrimp shell powder, 100 parts of sweet potato stems and leaves, 40 parts of corns and 3 parts of glucose;
(2) hastening parturition before breeding: adding oxytocin into feed for feeding for a period of time, simultaneously increasing white light irradiation, and adding an ovulation promoting agent into the feed of later-stage female fish, wherein the white light irradiation is 120Lx white light irradiation for 3 hours, the oxytocin is chorionic gonadotropin, the addition amount of the oxytocin is 0.5 percent of the mass of the feed, and the ovulation promoting agent is added into the feed for feeding after the oxytocin addition is stopped; the ovulation promoting agent is prepared by extracting semen Cuscutae 12 parts, radix Boehmeriae 8 parts, petiolus Nelumbinis 13 parts, and folium Artemisiae Argyi 7 parts with ethanol, concentrating into extract, adding luteinizing releasing hormone 1 part, adding corn flour 100 parts, mixing, air drying, and pulverizing;
(3) fertilization and propagation: mixing and feeding female pelteobagrus fulvidraco and male pelteobagrus fulvidraco, then dividing a water pool into two parts by using a fishing net, dividing all pelteobagrus fulvidraco into one side of a water inlet of the water pool for feeding, adding a spawning agent into water, keeping running water flowing, wherein the spawning agent is a plant spawning agent, diluting a fermentation liquid after plant leaves are fermented, and pouring the fermentation liquid into the water pool, wherein the plant leaves are olive leaves;
(4) hatching management: after the female pelteobagrus fulvidraco and the male pelteobagrus fulvidraco lay eggs, keeping the water temperature to 23 ℃, adjusting the pH value of the water to 7, pumping oxygen into the water pool, and slowly stirring to ensure that fertilized eggs are separated by breaking membranes as soon as possible, and after the fish eggs are fertilized and incubated, keeping the water temperature in the water pool at 28 ℃;
(5) seedling cultivation and screening: properly widening the breeding range of the hatched fries, then dissolving the fry breeding feed in water, introducing the dissolved fry breeding feed into water for feeding, providing a proper amount of white light irradiation, irradiating for 3 hours by using white light of 80Lx, extinguishing for 8 hours, continuing to irradiate, and screening the fries with different volumes after 20 days and separately breeding.
Example 3
An artificial breeding and seedling raising method for pelteobagrus fulvidraco comprises the following steps:
(1) parent screening: selecting pelteobagrus fulvidraco which is about to mature and large in the same period, feeding the female and male pelteobagrus fulvidraco separately and feeding the same feed, keeping the pelteobagrus fulvidraco fully mature for later use, breeding the pelteobagrus fulvidraco for 5-6 months after birth, keeping the weight of the pelteobagrus fulvidraco at 80-100g, cooking sweet potatoes, mashing and mixing the feed with bananas, adding shrimp shell powder, sweet potato stems and leaves, corn and glucose, fully crushing, mixing, airing and crushing again to form the feed; the feed comprises 100 parts of sweet potatoes, 30 parts of bananas, 45 parts of shrimp shell powder, 85 parts of sweet potato stems and leaves, 35 parts of corns and 2 parts of glucose;
(2) hastening parturition before breeding: adding oxytocin into feed for feeding for a period of time, simultaneously increasing white light irradiation, and adding an ovulation promoting agent into the feed of later-stage female fish, wherein the white light irradiation is performed for 2 hours by using 100Lx white light irradiation, the oxytocin is chorionic gonadotropin, the addition amount is 0.1% of the feed quality, and the ovulation promoting agent is added into the feed for feeding after the oxytocin addition is stopped; the ovulation promoting agent is prepared by extracting 11 parts of semen Cuscutae, 7 parts of radix Boehmeriae, 12 parts of petiolus Nelumbinis, and 6 parts of folium Artemisiae Argyi with ethanol, concentrating into extract, adding 0.5 part of luteinizing releasing hormone, adding 80 parts of corn flour, mixing, air drying, and pulverizing;
(3) fertilization and propagation: mixing and feeding female pelteobagrus fulvidraco and male pelteobagrus fulvidraco, then dividing a water pool into two parts by using a fishing net, dividing all pelteobagrus fulvidraco into one side of a water inlet of the water pool for feeding, adding a spawning agent into water, keeping running water flowing, wherein the spawning agent is a plant spawning agent, diluting a fermentation liquid after plant leaves are fermented, and pouring the fermentation liquid into the water pool, wherein the plant leaves are olive leaves;
(4) hatching management: after the female pelteobagrus fulvidraco and the male pelteobagrus fulvidraco lay eggs, keeping the water temperature to 20 ℃, adjusting the pH value of the water to 7, pumping oxygen into the water pool, and slowly stirring to ensure that fertilized eggs are separated by breaking membranes as soon as possible, and after the fish eggs are fertilized and incubated, keeping the water temperature in the water pool at 27 ℃;
(5) seedling cultivation and screening: properly widening the breeding range of the hatched fries, then dissolving the fry breeding feed in water, introducing the dissolved fry breeding feed into water for feeding, providing a proper amount of white light irradiation, irradiating for 2 hours by using white light of 60Lx, extinguishing for 6 hours, continuing to irradiate, screening fries with different volumes after 15 days, and separately breeding.
Comparative example 1
An artificial breeding and seedling raising method for pelteobagrus fulvidraco comprises the following steps:
(1) parent screening: selecting pelteobagrus fulvidraco which is about to mature and large in the same period, feeding the female and male pelteobagrus fulvidraco separately and feeding the same feed, keeping the pelteobagrus fulvidraco fully mature for later use, breeding the pelteobagrus fulvidraco for 5-6 months after birth, keeping the weight of the pelteobagrus fulvidraco at 80-100g, cooking sweet potatoes, mashing and mixing the feed with bananas, adding shrimp shell powder, sweet potato stems and leaves, corn and glucose, fully crushing, mixing, airing and crushing again to form the feed; the feed comprises 100 parts of sweet potatoes, 30 parts of bananas, 45 parts of shrimp shell powder, 85 parts of sweet potato stems and leaves, 35 parts of corns and 2 parts of glucose;
(2) hastening parturition before breeding: adding oxytocin into the feed for feeding for a period of time, and adding an ovulation promoting agent into the feed of later-stage female fish, wherein the oxytocin is chorionic gonadotropin, the addition amount of the oxytocin is 0.1% of the mass of the feed, and the ovulation promoting agent is added into the feed for feeding after the oxytocin addition is stopped; the ovulation promoting agent is prepared by extracting 11 parts of semen Cuscutae, 7 parts of radix Boehmeriae, 12 parts of petiolus Nelumbinis, and 6 parts of folium Artemisiae Argyi with ethanol, concentrating into extract, adding 0.5 part of luteinizing releasing hormone, adding 80 parts of corn flour, mixing, air drying, and pulverizing;
(3) fertilization and propagation: mixing and feeding female pelteobagrus fulvidraco and male pelteobagrus fulvidraco, then dividing a water pool into two parts by using a fishing net, dividing all pelteobagrus fulvidraco into one side of a water inlet of the water pool for feeding, adding a spawning agent into water, keeping running water flowing, wherein the spawning agent is a plant spawning agent, diluting a fermentation liquid after plant leaves are fermented, and pouring the fermentation liquid into the water pool, wherein the plant leaves are olive leaves;
(4) hatching management: after the female pelteobagrus fulvidraco and the male pelteobagrus fulvidraco lay eggs, keeping the water temperature to 20 ℃, adjusting the pH value of the water to 7, pumping oxygen into the water pool, and slowly stirring to ensure that fertilized eggs are separated by breaking membranes as soon as possible, and after the fish eggs are fertilized and incubated, keeping the water temperature in the water pool at 27 ℃;
(5) seedling cultivation and screening: the breeding range of the hatched fries is properly widened, then the fry breeding feed is dissolved in water and is guided into the water for feeding, and the fries with different volumes are screened and separately bred after 15 days.
Comparative example 2
An artificial breeding and seedling raising method for pelteobagrus fulvidraco comprises the following steps:
(1) parent screening: selecting pelteobagrus fulvidraco which is about to mature and large in the same period, feeding the female and male pelteobagrus fulvidraco separately and feeding the same feed, keeping the pelteobagrus fulvidraco fully mature for later use, breeding the pelteobagrus fulvidraco for 5-6 months after birth, keeping the weight of the pelteobagrus fulvidraco at 80-100g, cooking sweet potatoes, mashing and mixing the feed with bananas, adding shrimp shell powder, sweet potato stems and leaves, corn and glucose, fully crushing, mixing, airing and crushing again to form the feed; the feed comprises 100 parts of sweet potatoes, 30 parts of bananas, 45 parts of shrimp shell powder, 85 parts of sweet potato stems and leaves, 35 parts of corns and 2 parts of glucose;
(2) fertilization and propagation: mixing and feeding female pelteobagrus fulvidraco and male pelteobagrus fulvidraco, then dividing a water pool into two parts by using a fishing net, dividing all pelteobagrus fulvidraco into one side of a water inlet of the water pool for feeding, adding a spawning agent into water, keeping running water flowing, wherein the spawning agent is a plant spawning agent, diluting a fermentation liquid after plant leaves are fermented, and pouring the fermentation liquid into the water pool, wherein the plant leaves are olive leaves;
(3) hatching management: after the female pelteobagrus fulvidraco and the male pelteobagrus fulvidraco lay eggs, keeping the water temperature to 20 ℃, adjusting the pH value of the water to 7, pumping oxygen into the water pool, and slowly stirring to ensure that fertilized eggs are separated by breaking membranes as soon as possible, and after the fish eggs are fertilized and incubated, keeping the water temperature in the water pool at 27 ℃;
(4) seedling cultivation and screening: properly widening the breeding range of the hatched fries, then dissolving the fry breeding feed in water, introducing the dissolved fry breeding feed into water for feeding, providing a proper amount of white light irradiation, irradiating for 2 hours by using white light of 60Lx, extinguishing for 6 hours, continuing to irradiate, screening fries with different volumes after 15 days, and separately breeding.
Comparative example 3
An artificial breeding and seedling raising method for pelteobagrus fulvidraco comprises the following steps:
(1) parent screening: selecting pelteobagrus fulvidraco which is about to mature and large in the same period, feeding the female and male pelteobagrus fulvidraco separately and feeding the same feed, keeping the pelteobagrus fulvidraco fully mature for later use, breeding the pelteobagrus fulvidraco for 5-6 months after birth, keeping the weight of the pelteobagrus fulvidraco at 80-100g, cooking sweet potatoes, mashing and mixing the feed with bananas, adding shrimp shell powder, sweet potato stems and leaves, corn and glucose, fully crushing, mixing, airing and crushing again to form the feed; the feed comprises 100 parts of sweet potatoes, 30 parts of bananas, 45 parts of shrimp shell powder, 85 parts of sweet potato stems and leaves, 35 parts of corns and 2 parts of glucose;
(2) hastening parturition before breeding: adding oxytocin into feed for feeding for a period of time, simultaneously increasing white light irradiation, and adding an ovulation promoting agent into the feed of later-stage female fish, wherein the white light irradiation is performed for 2 hours by using 100Lx white light irradiation, the oxytocin is chorionic gonadotropin, the addition amount is 0.1% of the feed quality, and the ovulation promoting agent is added into the feed for feeding after the oxytocin addition is stopped; the ovulation promoting agent is prepared by extracting semen Cuscutae 11 parts, radix Boehmeriae 7 parts, petiolus Nelumbinis 12 parts, and folium Artemisiae Argyi 6 parts with ethanol, concentrating into extract, adding luteinizing releasing hormone 0.5 parts, adding corn flour 80 parts, mixing, air drying, and pulverizing;
(3) fertilization and propagation: the method comprises the following steps of (1) feeding female pelteobagrus fulvidraco and male pelteobagrus fulvidraco in a mixed mode, and keeping running water flowing;
(4) hatching management: after the female pelteobagrus fulvidraco and the male pelteobagrus fulvidraco lay eggs, keeping the water temperature to 20 ℃, adjusting the pH value of the water to 7, pumping oxygen into the water pool, and slowly stirring to ensure that fertilized eggs are separated by breaking membranes as soon as possible, and after the fish eggs are fertilized and incubated, keeping the water temperature in the water pool at 27 ℃;
(5) seedling cultivation and screening: properly widening the breeding range of the hatched fries, then dissolving the fry breeding feed in water, introducing the dissolved fry breeding feed into water for feeding, providing a proper amount of white light irradiation, irradiating for 2 hours by using white light of 60Lx, extinguishing for 6 hours, continuing to irradiate, screening fries with different volumes after 15 days, and separately breeding.
In the above examples, example 1 to example 3 were performed completely according to the operation steps of the present invention, comparative example 1 was performed without using white light irradiation, the rest of the operations were performed in accordance with example 3, comparative example 2 was performed without performing the step of hastening parturition before breeding, the rest of the operations were performed in accordance with example 3, comparative example 3 was performed in accordance with example 3 by directly mixing and feeding female and male pelteobagrus fulvidraco in step (3), and the rest of the operations were performed according to the 6 methods, respectively, and it was verified that the fertilization rate reached about 92%, the hatching rate reached about 90%, and the survival rate reached 80%, and the specific implementation data are shown in table 1.
TABLE 1 reproduction of the examples
| Example 1 | Example 2 | Example 3 | Comparative example 1 | Comparative example 2 | Comparative example 3 | |
| Female fish/tail | 103 | 98 | 102 | 102 | 99 | 105 |
| Average weight/g of female fish | 81.9 | 83.9 | 84.0 | 85.2 | 81.9 | 83.1 |
| Male fish/tail | 118 | 115 | 120 | 118 | 116 | 121 |
| Average weight/g of male fish | 96.2 | 91.5 | 96.3 | 96.2 | 94.1 | 93.8 |
| Total number of eggs (ten thousand grains) | 9.69 | 10.21 | 9.94 | 8.11 | 7.69 | 9.72 |
| Fertilization count (ten thousand grains) | 8.96 | 9.42 | 9.24 | 6.56 | 6.31 | 7.87 |
| Incubation total number (ten thousand grains) | 8.09 | 8.49 | 8.34 | 5.93 | 5.63 | 7.09 |
| Survival total number (Wan tailed) | 6.87 | 6.84 | 6.72 | 4.78 | 4.52 | 5.67 |
As can be seen from table 1, the fertilization rates, hatching rates and survival rates were all high in examples 1 to 3, the total number of eggs laid was low and the total number of fertilized eggs was low in comparative example 1 without light irradiation, but the subsequent hatchability was comparable to that of example 3 since the subsequent implementation was consistent with the present invention, the ratio of total number of eggs laid to total number of fertilized eggs was low in comparative example 2 since the step of hastening spawning before breeding was not performed, and the ratio of total number of eggs laid to total number of fertilized eggs was low in comparative example 3 since the step of isolating the female fish and the male fish eggs from the fish eggs was not performed and the total number of fertilized eggs and hatching were impaired in comparative example 3, pelteobagrus fulvidraco could be easily mass-bred only by operating the steps completely in accordance with the present invention, and no dedicated hatching facilities were required, the operation technique was simple, and breeding techniques, breeding rates and survival rates were easily mastered by farmers, Low propagation cost and easy large-area popularization and application.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that those skilled in the art can make various improvements and modifications without departing from the principle of the present invention, and these improvements and modifications should also be construed as the protection scope of the present invention.
Claims (5)
1. An artificial breeding and seedling raising method for pelteobagrus fulvidraco is characterized in that: the method comprises the following steps:
(1) parent screening: selecting pelteobagrus fulvidraco which is about to mature and is large in the same period, wherein the pelteobagrus fulvidraco in the same period is a pelteobagrus fulvidraco which is bred for 5-6 months after birth and has the weight of 80-100g, and female and male fishes are separately bred and fed with the same feed for standby after being completely mature;
(2) hastening parturition before breeding: adding oxytocin into feed for feeding for a period of time, simultaneously increasing white light irradiation, wherein the white light irradiation is 80-120Lx white light irradiation for 1-3h, adding an ovulation promoting agent into the feed of later-stage female fish, the oxytocin is chorionic gonadotropin, the addition amount of the oxytocin is 0.05-0.5% of the mass of the feed, the ovulation promoting agent is added into the feed for feeding after the oxytocin addition is stopped, the ovulation promoting agent is prepared by adding 10-12 parts of semen cuscutae, 6-8 parts of ramie root, 11-13 parts of lotus petiolus and 5-7 parts of folium artemisiae argyi into extract after alcohol extraction, concentrating the extract into luteinizing releasing hormone 0.1-1 part and adding 50-100 parts of corn flour, uniformly mixing, airing and crushing;
(3) fertilization and propagation: mixing and feeding female pelteobagrus fulvidraco and male pelteobagrus fulvidraco, then dividing a water pool into two parts by using a fishing net, dividing all pelteobagrus fulvidraco into one side of a water inlet of the water pool for feeding, adding a spawning agent into water, keeping running water flowing, wherein the temperature of the water in the water pool is 25-28 ℃, the spawning agent is a plant spawning agent, and fermenting through olive leaves, and then diluting a fermentation liquid and pouring the fermentation liquid into the water pool;
(4) hatching management: after the female pelteobagrus fulvidraco and the male pelteobagrus fulvidraco lay eggs, keeping the water temperature and adjusting the pH value in the water, and pumping oxygen into the water pool to wait for fertilization and incubation of fish eggs;
(5) seedling cultivation and screening: the breeding range of the hatched fries is properly widened, then the fry breeding feed is dissolved in water and is guided into the water for breeding, a proper amount of white light irradiation is provided, and the fries with different volumes are screened and separately bred after 10 to 20 days.
2. The artificial breeding and seedling raising method for pelteobagrus fulvidraco according to claim 1, which is characterized in that: in the step (1), the feed is prepared by cooking sweet potatoes, mashing and mixing the cooked sweet potatoes with bananas, adding shrimp shell powder, sweet potato stems and leaves, corn and glucose, completely crushing, mixing, airing and crushing again.
3. The artificial breeding and seedling raising method for pelteobagrus fulvidraco according to claim 2, which is characterized in that: in the step (1), the feed comprises, by weight, 80-120 parts of sweet potatoes, 10-50 parts of bananas, 20-60 parts of shrimp shell powder, 70-100 parts of sweet potato stems and leaves, 30-40 parts of corns and 1-3 parts of glucose.
4. The artificial breeding and seedling raising method for pelteobagrus fulvidraco according to claim 1, which is characterized in that: in the step (4), the water temperature is adjusted to 20-23 ℃, the pH value of the water is adjusted to 6-7, and the fertilized eggs are slowly stirred while pumping oxygen to break membranes and separate as soon as possible.
5. The artificial breeding and seedling raising method for pelteobagrus fulvidraco according to claim 1, which is characterized in that: in the step (5), the white light irradiation is performed for 1 to 3 hours by using white light irradiation of 50 to 80Lx, and the irradiation is continued after the white light irradiation is extinguished for 4 to 8 hours.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010155497.XA CN111296331B (en) | 2020-03-09 | 2020-03-09 | Artificial breeding and seedling raising method for pelteobagrus fulvidraco |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202010155497.XA CN111296331B (en) | 2020-03-09 | 2020-03-09 | Artificial breeding and seedling raising method for pelteobagrus fulvidraco |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN111296331A CN111296331A (en) | 2020-06-19 |
| CN111296331B true CN111296331B (en) | 2022-05-13 |
Family
ID=71151239
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202010155497.XA Active CN111296331B (en) | 2020-03-09 | 2020-03-09 | Artificial breeding and seedling raising method for pelteobagrus fulvidraco |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN111296331B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112042572A (en) * | 2020-09-18 | 2020-12-08 | 贵州裕和蔬菜种植农民专业合作社 | Method for hatching and breeding high back carps |
Citations (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1759813A (en) * | 2005-10-28 | 2006-04-19 | 李维贤 | Method for breeding golden thread |
| CN101664009A (en) * | 2009-06-29 | 2010-03-10 | 中国水产科学研究院淡水渔业研究中心 | Method for breeding finless eel in pond |
| CN102144595A (en) * | 2011-04-22 | 2011-08-10 | 四川英杰科技发展有限公司 | Breeding method of loach |
| CN103190537A (en) * | 2013-04-09 | 2013-07-10 | 林衍峰 | Fish oytocin oral granules and preparation and using methods thereof |
| CN103238563A (en) * | 2013-05-07 | 2013-08-14 | 赵道全 | Method for promoting female and male parent giant salamanders to synchronously mature |
| CN104431479A (en) * | 2014-11-27 | 2015-03-25 | 贾晓晨 | Turtle feed and preparation method thereof |
| CN106472823A (en) * | 2016-10-13 | 2017-03-08 | 合肥润雨农业科技有限公司 | A kind of auxiliary feedstuff of crucian cultivation |
| CN106804477A (en) * | 2015-11-30 | 2017-06-09 | 宁陕县绿康农业生态科技有限公司 | A kind of artificial fecundation method of Pelteobagrus fulvidraco |
| CN106923079A (en) * | 2017-02-20 | 2017-07-07 | 黄怀勇 | A kind of fish meal and preparation method thereof |
| CN107494344A (en) * | 2017-07-31 | 2017-12-22 | 兰溪市普润斯水产养殖技术有限公司 | A kind of artificial culturing method of advanced fry of yellow catfish |
| CN107980670A (en) * | 2017-11-29 | 2018-05-04 | 安徽金阳光农业服务有限公司 | A kind of Pelteobagrus fulvidraco induced spawning method |
| CN108834950A (en) * | 2018-07-09 | 2018-11-20 | 安徽省家牧动物营养科技有限公司 | A kind of cultural method promoting the development of ovary of Pelteobagrus fulvidraco female parent |
| CN110150187A (en) * | 2019-06-25 | 2019-08-23 | 浙江师范大学 | Middle Warsaw loach large-scale artificial mating system |
| CN110250054A (en) * | 2019-08-06 | 2019-09-20 | 安徽省农业科学院水产研究所 | A kind of parent culture method promoting Pelteobagrus fulvidraco gonad development |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2004062563A2 (en) * | 2003-01-16 | 2004-07-29 | Université de Lausanne | Methods for screening for therapeutic agents that are useful in the treatment of oxytocin and vasopressin-related disorders |
-
2020
- 2020-03-09 CN CN202010155497.XA patent/CN111296331B/en active Active
Patent Citations (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1759813A (en) * | 2005-10-28 | 2006-04-19 | 李维贤 | Method for breeding golden thread |
| CN101664009A (en) * | 2009-06-29 | 2010-03-10 | 中国水产科学研究院淡水渔业研究中心 | Method for breeding finless eel in pond |
| CN102144595A (en) * | 2011-04-22 | 2011-08-10 | 四川英杰科技发展有限公司 | Breeding method of loach |
| CN103190537A (en) * | 2013-04-09 | 2013-07-10 | 林衍峰 | Fish oytocin oral granules and preparation and using methods thereof |
| CN103238563A (en) * | 2013-05-07 | 2013-08-14 | 赵道全 | Method for promoting female and male parent giant salamanders to synchronously mature |
| CN104431479A (en) * | 2014-11-27 | 2015-03-25 | 贾晓晨 | Turtle feed and preparation method thereof |
| CN106804477A (en) * | 2015-11-30 | 2017-06-09 | 宁陕县绿康农业生态科技有限公司 | A kind of artificial fecundation method of Pelteobagrus fulvidraco |
| CN106472823A (en) * | 2016-10-13 | 2017-03-08 | 合肥润雨农业科技有限公司 | A kind of auxiliary feedstuff of crucian cultivation |
| CN106923079A (en) * | 2017-02-20 | 2017-07-07 | 黄怀勇 | A kind of fish meal and preparation method thereof |
| CN107494344A (en) * | 2017-07-31 | 2017-12-22 | 兰溪市普润斯水产养殖技术有限公司 | A kind of artificial culturing method of advanced fry of yellow catfish |
| CN107980670A (en) * | 2017-11-29 | 2018-05-04 | 安徽金阳光农业服务有限公司 | A kind of Pelteobagrus fulvidraco induced spawning method |
| CN108834950A (en) * | 2018-07-09 | 2018-11-20 | 安徽省家牧动物营养科技有限公司 | A kind of cultural method promoting the development of ovary of Pelteobagrus fulvidraco female parent |
| CN110150187A (en) * | 2019-06-25 | 2019-08-23 | 浙江师范大学 | Middle Warsaw loach large-scale artificial mating system |
| CN110250054A (en) * | 2019-08-06 | 2019-09-20 | 安徽省农业科学院水产研究所 | A kind of parent culture method promoting Pelteobagrus fulvidraco gonad development |
Non-Patent Citations (2)
| Title |
|---|
| "暹罗斗鱼人工繁育方式的研究";张希 等;《热带农业科学》;20121231;第32卷(第12期);第103-105页 * |
| "水温与光照对瓦氏黄颡鱼幼鱼行为的影响";王武 等;《生态学杂志》;20080531;第27卷(第5期);第791-796页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN111296331A (en) | 2020-06-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104857337B (en) | A kind of grass parent population fermented tcm microorganism formulation and preparation method thereof | |
| CN105613999B (en) | Improve breeder performance ferment and preparation method thereof | |
| CN105248320A (en) | Freshwater shrimp culture method | |
| CN106417122B (en) | A kind of breeding method improving high-quality freshwater shrimp seed yield | |
| CN110358819A (en) | A kind of breeding method of all-male crow spot hybridized snakehead fish | |
| CN111956783A (en) | Artificial spawning induction mixture for micropterus salmoides and artificial breeding method | |
| CN111296331B (en) | Artificial breeding and seedling raising method for pelteobagrus fulvidraco | |
| CN108935938A (en) | Promote cray husking breeding feed | |
| CN113040071B (en) | Method for cultivating fries of opsariichthys bidens | |
| CN108991292A (en) | Enhanced water resistance aquatic biological resisting stress food calling additive and preparation method | |
| CN106489799B (en) | The method that breeding is sheerly the full raun of Pelteobagrus fulvidraco and supermale fish large-scale production all-male fish | |
| CN108771013A (en) | Boar compound Synbiotics | |
| CN106912429A (en) | A kind of mating system of cray | |
| CN109673544B (en) | Artificial propagation technology for schizothorax grahami | |
| CN107198038B (en) | Chlorella powder and spirulina powder composition and feed prepared from composition and used for breeding healthy pigs | |
| CN107223617B (en) | Penaeus vannamei boone cultivation method capable of shortening cultivation period | |
| CN108935932A (en) | Mixed feed for cray cultivation | |
| CN108541632A (en) | A kind of sturgeon mating system | |
| CN109874707B (en) | Method for efficiently creating allooctaploid silver crucian carp | |
| CN111802291A (en) | Ecological breeding method for oversized parent of Chinese mitten crabs | |
| CN109169416A (en) | Pelteobagrus fulvidraco offspring seed cultivation method | |
| CN115380871B (en) | Cultivation method of golden-tomb green-shell laying hen | |
| CN109673540A (en) | A kind of hybridization Pelteobagrus fulvidraco batch artificial fecundation method | |
| CN111296332B (en) | Method for promoting development of female ovary of pelteobagrus fulvidraco | |
| CN104187043A (en) | Piglet feed improving intestines and the stomach and preparing method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |