CN103190537A - Fish oytocin oral granules and preparation and using methods thereof - Google Patents
Fish oytocin oral granules and preparation and using methods thereof Download PDFInfo
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention discloses fish oytocin oral granules and preparation and using methods thereof, and belongs to the technical field of fish culture. Aiming at the problems of difficult hormone delivery, complex operation, high cost, low ecbolic efficiency, high parent fish death rate and the like in artificial propagation of certain small fishes, the invention provides an artificial ecbolic method, which avoids fish stress response death and is high in ecbolic rate, easy, convenient, feasible and low in cost. The method comprises the following steps of: dissolving oytocin into normal saline, spraying the solution to an aquatic granulated feed, preparing the fish oytocin oral granules by wrapping a layer of animal grease containing vitamin E on the surface of the aquatic granulated feed, feeding the fishes with the oytocin oral granules in the propagation season of the fishes, and delivering the external hormone to parent fishes when the fishes actively eat the oral granules to induce gonad maturation, laying and fertilization of the parent fishes. The oral granules and the method are mainly applied to the artificial propagation of certain small fishes.
Description
Technical field
The present invention relates to a kind of Technique in Fishes oxytocic hormone preparation, be specifically related to a kind of fish oxytocic hormone oral granule and preparation and using method, belong to the fish culture technical field.
Background technology
In artificial propagation of fish, by injection exogenous hormones is injected directly that to hasten parturition in the fish body be a kind of main means, exogenous hormones valid density in fish body weak point of holding time often needs multiple injection.The shortcoming of this method be fish for, fixing and injection process Mesichthyes stress reaction is strong, very big to the damage of fish body, some small fishes are because individuality is little, resistance is poor, and using injection to hasten parturition not only wastes time and energy, and the parent population death rate is very high, and reproductive effect is poor.
Chinese patent CN101428140A discloses sustained-release incretion particle of a kind of inducing maturation of Oplegnathus fasciatus gonad and preparation method thereof, after will releasing control thing (cholesterol, cellulose), luteinizing hormone releasing hormone and maleic acid DOM and evenly mixing by described content, make sustained-release incretion particle, implant the disposable administration of female oplegnathus fasciatus muscle of back (perhaps adopting oral other modes that waits) with the implantation ware.The shortcoming of this method is to need grains dedicated mould and aseptic drying equipment, complex process in the sustained-release incretion particle preparation; Still need injection operation, can not solve the small fishes high problem of the death rate of hastening parturition; The sustained-release incretion particle feeding patterns is owing to Main Ingredients and Appearance in the particle is hormone and cholesterol, and fish are not liked and ingest, and throw something and feed and can not play the effect of hastening parturition.
Chinese patent CN102177863A discloses the method for hastening parturition that hormone soaks loach, with maleic acid DOM, luteotropin releasing hormone d-ala analog and human chorionic gonadtropin three's mixture as oxytocic hormone, adding ethyl ketone theobromine and edible oil mixes and makes soak in physiological saline the loach parent is soaked, the control of soak temperature is at 21～29 ℃, soak time control was at 0.5～8 hour, and spawning rate can be controlled in 20%～100%.The shortcoming of this method is to need higher water temperature or long soak time, the hormone use amount is big, cost is high, and some need that long-time hormone induction just can reach gonad maturity and require the streams property fish of height, non-refractory can not tolerate this method to water quality, flow velocity, dissolved oxygen.
Chinese patent CN101933469A discloses the method for a kind of artificial batch breeding swamp eel seed, female, male close eel was all stopped eating 1 day before hastening parturition, human chorionic gonadtropin and luteotropin releasing hormone d-ala analog are mixed into the oxytocic hormone soup in physiological saline, be injected in the earthworm body cavity by the 2ml/ bar, every tail parent eel 1 earthworm that contains hormone of throwing something and feeding is hastened parturition, 3～4 days in-laws' eel Tu Pao breeding of nesting, swamp eel parent's spawning rate reaches 80～90%.The shortcoming of this method be oxytocic hormone is injected in the earthworm body waste time and energy, operation easier is big, earthworm is discharged the part hormone in response to swashing with Excretion, some streams property small fishes parents whole piece earthworm that can not disposablely ingest is difficult to precisely control hormone dosage, the spawning rate instability.
Summary of the invention
Technical problem to be solved by this invention is, a kind of avoid the causing death of fish stress reaction, spawning rate height, induced spawning method simple and easy to do, with low cost are provided.
In order to solve the problems of the technologies described above, the invention provides a kind of fish oxytocic hormone oral granule and preparation and using method, realize by the following technical solutions.
A kind of fish oxytocic hormone oral granule, it is characterized in that: be carrier with the aquatic granulated feed, absorb fish oxytocic hormone solution, the aquatic granulated feed surface coats animal fat and forms watertight composition, the oxytocic hormone dissolving is lost in the water body when avoiding throwing something and feeding, add vitamin E in the animal fat and prevent that Oxidation of Fat and Oils is rotten, the effect of each component is:
(1) fish oxytocic hormone: the effect that has the inductivity gland maturity and hasten parturition, commonly used is luteotropin releasing hormone d-ala analog, human chorionic gonadtropin and fish hypophysis, can be used alone or as a mixture;
(2) maleic acid DOM: the effect of hastening parturition that can improve hormone;
(3) aquatic granulated feed: the carrier of hormone also is the source of nutrition that fish growth is grown, and has grit to sink forage, buoyancy expanding pellet and heavy property expanded pellet feed, the kind model specification of selecting for use parent population to throw something and feed usually;
(4) animal fat: the sealing impermeable agent of particle, select chicken fat or duck oil for use, its fusing point is 22 ℃-28 ℃;
(5) vitamin E: prevent that Oxidation of Fat and Oils is rotten, the effect that promotes gonad development is arranged;
(6) physiological saline: dissolving and dispersion hormone.
A kind of preparation method of fish oxytocic hormone oral granule is characterized in that: hormone is dissolved in the physiological saline, is sprayed on the pellet and dries, add the animal fat that contains vitamin E and stir, comprise following steps:
(1) hormone, vitamin E, animal fat are put in 2～4 ℃ of refrigerating boxes and are preserved, physiological saline, pellet be cooled to 2～4 ℃ standby;
(2) hormone and physiological saline are made solution;
(3) aquatic granulated feed is divided on the plane that does not absorb water, and about 2～3 feeds of thickness do not interspace;
(4) with micro-sprayer hormone solution evenly is sprayed on the aquatic granulated feed, sprayed half hormone solution after, aquatic granulated feed stirred spreads out again, sprayed remaining hormone solution, put the shady and cool ventilation place and allow soup absorb fully;
(5) animal fat is slowly heated to whole fusings, adds vitamin E and stirs;
(6) pellet that will contain hormone places plastic containers, adds the animal fat that contains vitamin E and stirs, and puts in 2～4 ℃ of refrigerating boxes and preserves;
(7) the fish oxytocic hormone oral granule resting period that is made into can not surpass 24 hours, in order to avoid drug effect descends.
A kind of using method of fish oxytocic hormone oral granule, it is characterized in that: at the fish reproduction fish oxytocic hormone oral granule of throwing something and feeding season, initiatively ingesting by fish is input to exogenous hormones in the parent population body, induces the parent population gonadal maturation and the insemination of laying eggs, and comprises following steps:
(1) strictness bait throwing at regular time and quantity in the parent population breeding process forms the custom of robbing food fast;
(2) keep flowing water or miniflow water to stimulate in the parent population gonad development process;
(3) throw something and feed and stopped eating 1 day before the fish oxytocic hormone oral granule;
(4) throw something and feed 1 time with the fish oxytocic hormone oral granule that contains the low dosage hormone or repeatedly, induce the parent population gonadal maturation spawning season 15～45 days in advance;
(5) behind the parent population gonadal maturation, the fish oxytocic hormone oral granule that contains the dosage of normally hastening parturition of throwing something and feeding is hastened parturition, then the fish nest is put into spawning pond, keep flowing water or miniflow water in the pond, arrive effect after the time parent population row of namely laying eggs voluntarily smart, the fish nest that need in time will stick embryonated egg in the process of laying eggs moves in the hatchery hatches, and prevents that parent population from eating.
A kind of fish oxytocic hormone oral granule provided by the invention and preparation thereof and using method are applicable to the artificial propagation of small fishes, are compared with the prior art, and beneficial effect is that preparation technology is simple, steady quality; The operation of hastening parturition is time saving and energy saving, need not to fish for and injection operation, and convenient drug administration has been avoided fish stress reaction and parent population death; Hormone runs off few, can accurately control the oxytocic hormone use amount, the effect stability of hastening parturition.
The specific embodiment
Below in conjunction with embodiment the present invention is described in further detail, but the specific embodiment of the present invention is not limited to following examples, under the prerequisite that does not exceed claim of the present invention, the present invention can also make the embodiment of optimization, replacement or the change of other various ways.
Example one: a kind of loach oxytocic hormone oral granule, every kilogram of oxytocic hormone oral granule contains the grains dedicated feed 930g of loach, human chorionic gonadtropin 150000IU, No. 3 800 μ g of luteotropin releasing hormone d-ala analog, maleic acid DOM 600mg, physiological saline 50ml, duck oil 20g, vitamin e1 0mg.
A kind of preparation of loach oxytocic hormone oral granule comprises following steps:
(1) hormone, vitamin E, animal fat are put in 2～4 ℃ of refrigerating boxes and are preserved, physiological saline, pellet be cooled to 2～4 ℃ standby;
(2) hormone and physiological saline are made solution;
(3) pellet is divided on the plane that does not absorb water, and about 2～3 feeds of thickness do not interspace;
(4) with micro-sprayer hormone solution evenly is sprayed on the pellet, sprayed half hormone solution after, pellet stirred spreads out again, sprayed remaining hormone solution, put the shady and cool ventilation place and allowed soup absorb fully in 30 minutes;
(5) animal fat is slowly heated to whole fusings, adds vitamin E and stirs;
(6) pellet that will contain hormone places plastic containers, adds the animal fat that contains vitamin E and stirs, and puts in 2～4 ℃ of refrigerating boxes and preserves;
(7) the fish oxytocic hormone oral granule resting period that is made into can not surpass 24 hours, in order to avoid drug effect descends.
A kind of using method of loach oxytocic hormone oral granule comprises following steps:
(1) strictness bait throwing at regular time and quantity in the parent population breeding process forms the custom of robbing food fast;
(2) keep miniflow water to stimulate in the parent population gonad development process;
(3) April, water temperature reached more than 22 ℃, when the parent population food ration descends suddenly, illustrated that sexual gland reaches maturity, and can arrange to hasten parturition;
(4) stopped eating 1 day before hastening parturition;
(5) by 1% of the parent population body weight oxytocic hormone oral granule of throwing something and feeding in the afternoon, then the fish nest is put into parent fish pond miniflow aqua region.During 22～25 ℃ of water temperatures, the parent population row of namely laying eggs voluntarily is smart after 13～15 hours, and the fish nest that needs in time will to stick embryonated egg in the process of laying eggs moves in the hatchery hatches, and prevents that parent population from eating.
Example two: a kind of silver-colored silver xenocypris oxytocic hormone oral granule, every kilogram of oxytocic hormone oral granule contains swelling granular buoyancy feed 930g, carp brain hypophysis 300mg, physiological saline 50ml, chicken fat 20g, vitamin e1 0mg.
A kind of preparation method of silver-colored silver xenocypris oxytocic hormone oral granule and step are with example one.
A kind of using method of silver-colored silver xenocypris oxytocic hormone oral granule and step are with example one.
Example three: No. 1, a kind of smooth lip fish oxytocic hormone oral granule, be used for inducing the parent population gonad maturity, every kilogram of light lip fish oxytocic hormone oral granule contains heavy property expanded pellet feed 930g, luteotropin releasing hormone d-ala analog No. 3 100 μ g, physiological saline 50ml, duck oil 20g, vitamin e1 00mg for No. 1.
No. 2, a kind of smooth lip fish oxytocic hormone oral granule, every kilogram of light lip fish oxytocic hormone oral granule contains heavy property expanded pellet feed 930g, human chorionic gonadtropin 100000IU, maleic acid DOM 150mg, physiological saline 50ml, duck oil 20g, vitamin e1 00mg for No. 2, is used for inducing parent population ovulation insemination.
A kind of preparation method of smooth lip fish oxytocic hormone oral granule and step are with example one.
A kind of using method of smooth lip fish oxytocic hormone oral granule comprises following steps:
(1) strictness bait throwing at regular time and quantity in the parent population breeding process forms the custom of robbing food fast;
(2) keep the depth of water 0.5～0.6m, flow velocity 0.1～0.2m/s in the parent population gonad development process;
Beginning at the beginning of (3) 4 months by throw something and feed between the lights No. 1, light lip fish oxytocic hormone oral granule of 1% of parent population body weight, is thrown something and fed weekly 1 time;
(4) expand and to arrange to hasten parturition when obvious pearl star appears in the corners of the mouth when the gorgeous corners of the mouth of milter body colour is covered with pearl star, raun belly June, stop eating before hastening parturition and control the depth of water 1～1.2m, stimulated 2 days with the current of flow velocity 0.3～0.5m/s;
Suspend flowing water (5) the 3rd day morning, by throw something and feed No. 2, light lip fish oxytocic hormone oral granule of 1% of parent population body weight, after ingesting and finish, parent population recovers flowing water, control flow velocity 0.2～0.3m/s, water level slowly is down to 0.4～0.5m, and the shallow box that rubble is housed is put into the pond, and morning next day, parent population was namely in the rubble insemination of laying eggs, need in time embryonated egg to be moved in the hatchery in the process of laying eggs to hatch, prevent that parent population from eating.
Claims (4)
1. a fish oxytocic hormone oral granule and preparation and using method, exogenous hormones is mixed with certain formulation, be input in the parent population body, induce the parent population gonadal maturation and the insemination of laying eggs, it is characterized in that the fish oxytocic hormone is mixed with oral granule, be input in the parent population body by the method for throwing something and feeding, induce the parent population gonadal maturation and the insemination of laying eggs.
2. a kind of fish oxytocic hormone oral granule according to claim 1 is characterized in that being made up of fish oxytocic hormone, aquatic granulated feed, animal fat and vitamin E.
3. the preparation method of a kind of fish oxytocic hormone oral granule according to claim 1, it is characterized in that hormone is dissolved in the physiological saline, be sprayed on the aquatic granulated feed and dry, add the animal fat contain vitamin E and stir and make, comprise following steps:
(1) hormone, vitamin E, animal fat are put in 2～4 ℃ of refrigerating boxes and are preserved, physiological saline, aquatic granulated feed be cooled to 2～4 ℃ standby;
(2) hormone and physiological saline are made solution;
(3) aquatic granulated feed is divided on the plane that does not absorb water, and about 2～3 feeds of thickness do not interspace;
(4) with micro-sprayer hormone solution evenly is sprayed on the aquatic granulated feed, sprayed half hormone solution after, aquatic granulated feed stirred spreads out again, sprayed remaining hormone solution, put the shady and cool ventilation place and allow soup absorb fully;
(5) animal fat is slowly heated to whole fusings, adds vitamin E and stirs;
(6) aquatic granulated feed that will contain hormone places plastic containers, adds the animal fat that contains vitamin E and stirs, and puts in 2～4 ℃ of refrigerating boxes and preserves;
The fish oxytocic hormone oral granule resting period of (7) making can not surpass 24 hours, in order to avoid drug effect descends.
4. the using method of a kind of fish oxytocic hormone oral granule according to claim 1, it is characterized in that at the fish reproduction fish oxytocic hormone oral granule of throwing something and feeding season, initiatively ingest by fish exogenous hormones is input in the parent population body, induce the parent population gonadal maturation and the insemination of laying eggs, comprise following steps:
(1) strictness bait throwing at regular time and quantity in the parent population breeding process forms the custom of robbing food fast;
(2) keep flowing water or miniflow water to stimulate in the parent population gonad development process;
(3) throw something and feed and stopped eating 1 day before the fish oxytocic hormone oral granule;
(4) throw something and feed 1 time with the fish oxytocic hormone oral granule that contains the low dosage hormone or repeatedly, induce the parent population gonadal maturation spawning season 15～45 days in advance;
(5) behind the parent population gonadal maturation, the fish oxytocic hormone oral granule of throwing something and feeding and containing the dosage of normally hastening parturition, then the fish nest is put into spawning pond, keep flowing water or miniflow water in the pond, arrive effect after the time parent population row of namely laying eggs voluntarily smart, the fish nest that need in time will stick embryonated egg in the process of laying eggs moves in the hatchery hatches, and prevents that parent population from eating.
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Cited By (9)
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| CN104206324A (en) * | 2014-08-07 | 2014-12-17 | 广东省实验动物监测所 | Gonad development and breeding method for mugilogobius chulae or ctenogobius gymnauchen |
| CN105724289A (en) * | 2016-02-26 | 2016-07-06 | 黄山市鼎新生态农业发展有限公司 | Efficient artificial propagation method for acrossocheilus fasciatus |
| CN105851705A (en) * | 2016-04-29 | 2016-08-17 | 宿松县龙源水产养殖专业合作社 | Fertilization assisting medicine liquid special for loaches |
| CN108371124A (en) * | 2018-04-08 | 2018-08-07 | 湖南文理学院 | The artificial biological control method of pseudorasbora parva |
| CN110169377A (en) * | 2019-06-27 | 2019-08-27 | 浙江海洋大学 | The artificial induced spawning fertilization method of spotted maigre |
| CN110250054A (en) * | 2019-08-06 | 2019-09-20 | 安徽省农业科学院水产研究所 | A kind of parent culture method promoting Pelteobagrus fulvidraco gonad development |
| CN110622892A (en) * | 2019-10-31 | 2019-12-31 | 宁德市富发水产有限公司 | Water-flowing type production promoting method for oplegnathus fasciatus and juvenile fish culturing method |
| CN111296331A (en) * | 2020-03-09 | 2020-06-19 | 广西壮族自治区水产科学研究院 | Artificial breeding and seedling raising method for pelteobagrus fulvidraco |
| CN111642657A (en) * | 2020-07-08 | 2020-09-11 | 天津现代晨辉科技集团有限公司 | Efficient oxytocic as well as preparation method and use method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104206324A (en) * | 2014-08-07 | 2014-12-17 | 广东省实验动物监测所 | Gonad development and breeding method for mugilogobius chulae or ctenogobius gymnauchen |
| CN105724289A (en) * | 2016-02-26 | 2016-07-06 | 黄山市鼎新生态农业发展有限公司 | Efficient artificial propagation method for acrossocheilus fasciatus |
| CN105724289B (en) * | 2016-02-26 | 2018-07-17 | 黄山市鼎新生态农业发展有限公司 | A kind of smooth lip fish high-efficiency artificial propagation method |
| CN105851705A (en) * | 2016-04-29 | 2016-08-17 | 宿松县龙源水产养殖专业合作社 | Fertilization assisting medicine liquid special for loaches |
| CN108371124A (en) * | 2018-04-08 | 2018-08-07 | 湖南文理学院 | The artificial biological control method of pseudorasbora parva |
| CN110169377B (en) * | 2019-06-27 | 2021-09-14 | 浙江海洋大学 | Artificial induced spawning and fertilization method for nibea albiflora |
| CN110169377A (en) * | 2019-06-27 | 2019-08-27 | 浙江海洋大学 | The artificial induced spawning fertilization method of spotted maigre |
| CN110250054A (en) * | 2019-08-06 | 2019-09-20 | 安徽省农业科学院水产研究所 | A kind of parent culture method promoting Pelteobagrus fulvidraco gonad development |
| CN110622892A (en) * | 2019-10-31 | 2019-12-31 | 宁德市富发水产有限公司 | Water-flowing type production promoting method for oplegnathus fasciatus and juvenile fish culturing method |
| CN110622892B (en) * | 2019-10-31 | 2022-02-11 | 宁德市富发水产有限公司 | Water-flowing type production promoting method for oplegnathus fasciatus and juvenile fish culturing method |
| CN111296331A (en) * | 2020-03-09 | 2020-06-19 | 广西壮族自治区水产科学研究院 | Artificial breeding and seedling raising method for pelteobagrus fulvidraco |
| CN111296331B (en) * | 2020-03-09 | 2022-05-13 | 广西壮族自治区水产科学研究院 | Artificial breeding and seedling raising method for pelteobagrus fulvidraco |
| CN111642657A (en) * | 2020-07-08 | 2020-09-11 | 天津现代晨辉科技集团有限公司 | Efficient oxytocic as well as preparation method and use method thereof |
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| CN103190537B (en) | 2014-10-29 |
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