CN110622892A - Water-flowing type production promoting method for oplegnathus fasciatus and juvenile fish culturing method - Google Patents

Water-flowing type production promoting method for oplegnathus fasciatus and juvenile fish culturing method Download PDF

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Publication number
CN110622892A
CN110622892A CN201911056221.XA CN201911056221A CN110622892A CN 110622892 A CN110622892 A CN 110622892A CN 201911056221 A CN201911056221 A CN 201911056221A CN 110622892 A CN110622892 A CN 110622892A
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water
days
parent fish
fish
feeding
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CN110622892B (en
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陈佳
刘兴彪
刘志民
柯巧珍
余训凯
黄匡南
翁华松
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Ningde Fufa Aquatic Products Co Ltd
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Ningde Fufa Aquatic Products Co Ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • A01K61/10Culture of aquatic animals of fish
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • A01K61/10Culture of aquatic animals of fish
    • A01K61/17Hatching, e.g. incubators
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K63/00Receptacles for live fish, e.g. aquaria; Terraria
    • A01K63/04Arrangements for treating water specially adapted to receptacles for live fish
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K63/00Receptacles for live fish, e.g. aquaria; Terraria
    • A01K63/04Arrangements for treating water specially adapted to receptacles for live fish
    • A01K63/042Introducing gases into the water, e.g. aerators, air pumps
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

Abstract

The invention relates to a flow-type production promoting method for oplegnathus fasciatus and a juvenile fish culturing method, belonging to the technical field of fish and aquatic organism culture in aquaculture. The running-type production promoting method of oplegnathus fasciatus comprises the following steps: 1) placing the sterilized parent fish into a parent fish pond to obtain domesticated parent fish; 2) in the reproduction period of the oplegnathus fasciatus, continuous local jet flow is provided for 15-20 days at the water level of the middle layer and the lower layer, so that mating of parent fish is completed, and the parent fish ovulates; 3) and (3) raising the water level of the parent fish pond, enabling the floating ova to flow out of the overflow hole of the parent fish pond, arranging a baffle at the overflow hole to drain the floating ova to an overflow pipeline, and collecting to obtain the oplegnathus fasciatus ova. The method can greatly improve the fertilized egg yield of oplegnathus fasciatus, improve the survival rate of the oplegnathus fasciatus in the larval stage and achieve the purpose of mass production.

Description

Water-flowing type production promoting method for oplegnathus fasciatus and juvenile fish culturing method
Technical Field
The invention relates to the technical field of fish and aquatic organism culture in aquaculture, in particular to a method for promoting the production of oplegnathus fasciatus in a flowing mode and a method for cultivating fish larvae.
Background
Oplegnathus fasciatus (Oplegnathus fasciatus), belonging to the order Perciformes, lithopteridae (Oplegnathidae), lithopterus fasciatus (Oplegnathus), is a tropical offshore fish. The oplegnathus fasciatus has beautiful body shape, bright color, tender meat and high nutritive value, is a seawater fish with high economic value and ornamental value, and has the market price as high as 300 yuan/kg. Through the research of artificial breeding technology, the strain is hopefully developed into a new excellent variety for factory culture, seawater cage culture, leisure fishery and proliferation and releasing in China. At present, the research on parent fish breeding and propagation technology of oplegnathus fasciatus is developed domestically, and the successful fry breeding is tested in a small range, but a stable industrial technology is not formed, and only a small amount of commercial fishes are sold in the market. The main reasons are that the obtaining rate of fertilized eggs of the oplegnathus fasciatus is low, the breeding difficulty of the oplegnathus fasciatus in the larval stage is high, and the yield of the oplegnathus fasciatus is low and unstable in the breeding production process of the oplegnathus fasciatus fry.
Disclosure of Invention
The invention aims to provide a method for breeding the young oplegnathus fasciatus. The method can greatly improve the fertilized egg yield of oplegnathus fasciatus, improve the survival rate of the oplegnathus fasciatus in the larval stage and achieve the purpose of mass production.
The invention provides a flowing water type production promoting method for oplegnathus fasciatus, which comprises the following steps:
1) putting the sterilized parent fish into a parent fish pond for temporary culture for 1d, feeding feed, changing water or adding water and absorbing dirt every day from the 2 nd day, and obtaining domesticated parent fish after 30-40 d;
2) in the reproduction period of the oplegnathus fasciatus, continuous local jet flow is provided for 15-20 days at the water level of the middle layer and the lower layer, so that mating of parent fish is completed, and the parent fish ovulates;
3) and (3) water is fed, so that the water level of the parent fish pond rises, the floating eggs flow out from the overflow holes of the parent fish pond, a baffle is arranged at the overflow hole to drain the floating eggs to an overflow pipeline, and the floating eggs are collected outside the overflow pipeline by using a water tank sleeved with a bolting silk net to obtain the oplegnathus fasciatus eggs.
Preferably, the density of the parent fish is 3-5Tail/m3
Preferably, the seawater used for the first time in the parent fish pond, the water for changing or adding water in the step 1) and the water for feeding water in the step 3) independently comprise a pretreatment process; the pretreatment comprises precipitation, sand filtration, disinfection and chlorine-eliminating aeration.
Preferably, the amount of the water change or the water addition in the step 1) is 20-30% of the volume of the original hydrophilic pool water.
The invention also provides a method for culturing the young sparus fasciatus in the juvenile fish stage based on the production promoting method of the technical scheme, which comprises the following steps:
culturing the oplegnathus fasciatus eggs obtained by the technical scheme, keeping the water temperature at 22-24 ℃, the salinity at 28-30, the pH value at 7.8-8.2, the ammonia nitrogen value below 0.3mg/L and the light intensity at 1000-2000 Lux in the whole culturing process, adding chlorella liquid every day to enable the concentration of chlorella in water to be 10-30 ten thousand/ml, continuously inflating, and starting a water pump provided with a seedling absorption prevention device to keep local underwater jet flow;
feeding rotifer brachypomus at the age of 2-10 days, feeding artemia nauplii at the age of 11-20 days, feeding micro-capsule feed at the age of 20 days in a transitional mode, feeding living copepods and nauplii at the age of 9-30 days, and applying rotifer reinforcer before the age of 30 days.
Preferably, in the cultivation process, the cultivation density is as follows: larval stage of 0.5-1.0 ten thousand tails/m3(ii) a 0.3-0.5 ten thousand tails/m in the juvenile fish period3(ii) a Young fish of over 45 days old with 0.1-0.2 ten thousand tails/m3
Preferably, the continuous aeration is 0.1-0.5L/min before 10 days of age, and 2-10L/min later.
Preferably, the diameter of the microcapsule feed is 0.2-0.5 μm, the average sinking speed in water is less than 0.1m/min, and 5% of particles can be suspended for 2-3 h.
Preferably, in the culturing process, the water amount is changed by 5% every day from 3 days within 20 days of age, and the dirt is sucked 1 time every week; the water exchange amount is 20-30% after 20 days of age, and the water exchange amount is 30-50% after 45 days of age.
Preferably, the seedling absorption prevention device is a sphere with the diameter of 0.5 m, and 80-mesh bolting silk meshes are densely distributed.
The invention provides a flow-type production promoting method for oplegnathus fasciatus and a method for culturing fish larvae. The method can greatly improve the fertilized egg yield of oplegnathus fasciatus, improve the survival rate of the oplegnathus fasciatus in the larval stage and achieve the purpose of mass production. Test results show that compared with the traditional method, the fertility method of the invention increases the output of fertilized eggs from 0.23kg to 2.7 kg; the hatching rate is more than 20%. The method has the advantages of high survival rate of the fries, high body length, low loss rate and high fish body quality.
Detailed Description
The invention provides a flowing water type production promoting method for oplegnathus fasciatus, which comprises the following steps:
1) putting the sterilized parent fish into a parent fish pond for temporary culture for 1d, feeding feed, changing water or adding water and absorbing dirt every day from the 2 nd day, and obtaining domesticated parent fish after 30-40 d;
2) in the reproduction period of the oplegnathus fasciatus, continuous local jet flow is provided for 15-20 days at the water level of the middle layer and the lower layer, so that mating of parent fish is completed, and the parent fish ovulates;
3) and (3) water is fed, so that the water level of the parent fish pond rises, the floating eggs flow out from the overflow holes of the parent fish pond, a baffle is arranged at the overflow hole to drain the floating eggs to an overflow pipeline, and the floating eggs are collected outside the overflow pipeline by using a water tank sleeved with a bolting silk net to obtain the oplegnathus fasciatus eggs.
The method comprises the steps of temporarily culturing the sterilized parent fish in a parent fish pond for 1d, feeding feed, changing water or adding water and absorbing dirt every day from the 2 nd day, and obtaining the domesticated parent fish after 30-40 d. In the invention, the parent fish is preferably oplegnathus fasciatus which is complete in body shape, normal in color and luster, strong, non-injurious, active in movement, more than 2-year-old and more than 550g in body mass. The source of the oplegnathus fasciatus is not particularly limited, and the commercially available oplegnathus fasciatus can be used. The method for disinfecting the parent fish is not particularly limited, and the conventional method for disinfecting the oplegnathus fasciatus, which is well known to those skilled in the art, can be adopted. In the invention, the temporary culture is preferably carried out by an ecological simulation method, preferably, the water is quiet and kept dark, the water depth is controlled to be 1.5-2.0 m, the water temperature is 22-23 ℃, the flowing water condition is set to be that the flow of a single water pump is 30-60L/min, and the water is fed according to the size of a poolDifferent numbers of water pumps are installed, and the water flows are uniformly sprayed in the anticlockwise direction or the clockwise direction. In the invention, the density of parent fish in the parent fish pond is preferably 3-5 tails/m3Preferably, the population remains > 100 tails. In the invention, the seawater used for the first time in the parent fish pond comprises a pretreatment process; the pretreatment comprises precipitation, sand filtration, disinfection and chlorine-eliminating aeration, and specifically comprises the following steps: through precipitation, sand filtration and disinfection with 10ppm bleaching powder for over 24h, then neutralization with sodium thiosulfate of the same concentration and aeration for about 4h, the environment is kept quiet. In the invention, the oplegnathus fasciatus entering the parent fish pond is fed with bait in 2d, wherein the bait is preferably palatable whole-grain oyster meat and palatable blocky frozen decapterus maruadsi, and in the invention, the feeding amount is preferably from less to more, and the feeding amount is 5% of the fish weight until the day. Specifically, the feeding amount of the 1 st feeding is limited to 1 grain per tail, and then the feeding amount is increased day by day according to the feeding condition. In the present invention, it is preferable that multivitamins for fish or antibiotics for fish be added to the bait for feeding. In the present invention, the feeding is preferably performed in the morning, the soil pick-up is preferably performed in the evening, and the water change or addition is also preferably performed in the evening. In the invention, the water changing or adding amount is preferably 20-30% of the volume of the original hydrophilic pool water. The water for changing or adding water preferably also comprises a pretreatment process; the pretreatment comprises precipitation, sand filtration, disinfection and chlorine-eliminating aeration, which is the same as the above. When the parent fish can move in the same direction, the parent fish can float upwards to snatch food when feeding, no dead fish record is recorded for many days continuously, and the domesticated parent fish is obtained after the record meets the breeding standard.
After the domesticated parent fish is obtained, the invention provides continuous local jet flow for 15-20 days at the water level of the middle and lower layers in the reproductive period of the oplegnathus fasciatus, so that the mating of the parent fish is completed, and the parent fish ovulates. The reproductive period of the oplegnathus fasciatus is preferably 4-5 months per year, the color change of sexually mature male and female fishes is observed, the body color of the common female fishes is relatively dark, the body fullness before spawning is increased, the gonad protrusion can be observed from the body surface, and if the oplegnathus fasciatus is touched by hands, the oplegnathus fasciatus has soft and elastic feeling; the gonad of the male individual does not protrude, and the milky thick seminal fluid flows out when the male individual lightly presses the abdomen, is linear in water and quickly disperses. The invention preferably stabilizes water at 22 ℃. The continuous localized jets provided by the lower water level in the present invention are preferably provided by a plurality of low power small water pumps deployed at the bottom and walls of the basin. The continuous local jet flow provided by the invention can stimulate the sexually mature parent fish of oplegnathus fasciatus to form mating behavior, so that the parent fish can naturally ovulate.
After the ovulation of the parent fish is completed, water is fed in to enable the water level of the parent fish pond to rise, the floating eggs flow out of an overflow hole of the parent fish pond, a baffle is arranged at the overflow hole to drain the floating eggs to an overflow pipeline, and the floating eggs are collected outside the overflow pipeline by a water tank sleeved with a bolting silk net to obtain the oplegnathus fasciatus eggs. In the present invention, the water feeding is preferably performed slowly. In the invention, the overflow hole is preferably provided with an opening along a distance of 25cm from one side wall of the parent fish pond, and the aperture of the overflow hole is preferably 20 cm. In the present invention, the influent water preferably includes a pretreatment process; the pretreatment comprises precipitation, sand filtration, disinfection and chlorine elimination aeration, and the specific conditions are the same as those described above.
The invention also provides a method for culturing the young sparus fasciatus in the juvenile fish stage based on the production promoting method of the technical scheme, which comprises the following steps:
after the oplegnathus fasciatus eggs are collected outside the overflow pipeline, the collected oplegnathus fasciatus fertilized eggs are preferably placed in a glass fiber reinforced plastic barrel to be precipitated and absorbed to remove settled eggs (dead eggs) at the bottom of the barrel, and then the floating eggs are collected at the rate of 0.5-1 ten thousand eggs/m3Is directly put in a density of more than 60m3Hatching in a cement pool of the water body. In the present invention, each 1.5m2~2.0m21 air diffusing stone is arranged at the bottom of the pool in area, and the pool is continuously and slightly inflated and is periodically changed with water. Avoid the sudden change of environment and the direct irradiation of sunlight, and stop air regularly to suck the dead eggs and dirt at the bottom. And (5) checking the hatching condition of the fertilized eggs in the hatching process, and observing the development condition of the embryos.
Culturing the oplegnathus fasciatus eggs obtained by the technical scheme, wherein in the whole culturing process, the water temperature is kept at 22-24 ℃, the preferred temperature is 22 ℃, the salinity is 28-30, the preferred salinity is 29, and mutation is avoided; the seawater is preferably subjected to dark precipitation and sand filtration, and is filtered into a pool by using a 250-mesh net bag, the pH value is 7.8-8.2, preferably 8.0, and the ammonia nitrogen value is below 0.3 mg/L; the method preferably regulates and controls the light intensity according to weather changes, so that the light intensity is 1000-2000 Lux, preferably 1800Lux, and sudden changes of the light intensity and direct sunlight are avoided; adding chlorella liquid every day to enable the concentration of chlorella in water to be 10-30 ten thousand per ml, the chlorella liquid is slightly green, and the ammonia nitrogen value and the transparency are reduced, wherein the chlorella liquid is preferably added every day at regular time, specifically, 3-5% of the total water is added every day at 8: 00-9: 00; continuously inflating to make the inflated bubbles fine without dead corner areas, and making the water-soluble oxygen of the pool reach more than 5ml/L at 0.1-0.5L/min before 10 days of age and 2-10L/min later; the method is characterized in that a water pump provided with a seedling absorption prevention device is started to keep local underwater jet flow, in the invention, the seedling absorption prevention device is a sphere with the diameter of 0.5 meter, and 80-mesh bolting silk meshes are densely distributed.
According to different requirements of different development stages of the larvae and the young fishes on nutrition and bait palatability, different bait varieties are preferably adopted to form a bait series for artificially culturing the larvae and the young fishes, rotifer brevicornus plicatilis is fed at the age of 2-10 days, the artemia nauplius is fed at the age of 11-20 days, the micro-capsule feed is fed at the age of 20 days in a transition mode, the living copepoda and the nauplius are fed at the age of 9-30 days, and the rotifer enhancer is applied before the age of 30 days. The microcapsule feed is fed in a transition mode from 20 days old, the diameter of the microcapsule feed is preferably 0.2-0.5 mu m, the average sinking speed in water is preferably less than 0.1m/min, 5% of particles can be suspended for 2-3 hours, and the addition of the microcapsule feed can effectively supplement complete nutrition in the process of fish larvae cultivation, replaces feeding of copepods, reduces the threat of exogenous diseases and drug residues brought by the copepods to fry production, and improves the survival rate of fry cultivation; the microcapsule feed can be used for domesticating young fishes and feeding young fishes, and is preferably fed with the microcapsule feed in the morning and then fed with other baits; the feeding method is preferably small in amount, multiple in times and slow in feeding, and the microcapsule feed is preferably fed in a water area with dense fry. The rotifer enhancer is applied before 30 days of age, and in the invention, the rotifer enhancer is preferably a Hengxing brand rotifer enhancer (emulsion) which enhances the nutrition of the rotifers in the pond, thereby enhancing the physique of the larvae.
In the present invention, the incubation is performed during the incubationThe cultivation density is as follows: larval stage of 0.5-1.0 ten thousand tails/m3(ii) a 0.3-0.5 ten thousand tails/m in the juvenile fish period3(ii) a Young fish of over 45 days old with 0.1-0.2 ten thousand tails/m3
In the invention, during the cultivation process, preferably within 20 days of age, starting from 3 days of age, the water amount is changed by 5% every day, and the dirt is sucked 1 time every week; the water exchange amount is 20-30% after 20 days of age, and the water exchange amount is 30-50% after 45 days of age. If the water quality is poor, intermittent running water can be considered.
The invention preferably carries out the fry separation when the larval fish has great size difference. The seedling dividing mode is not specially limited, and a conventional seedling dividing method is adopted.
The seedling emergence operation of the invention is preferably as follows: the average total length of the fry reaches more than 5.0cm, and the fry is transferred to a marine net cage for culture by using a running water transportation device.
The following describes the flow-type spawning promotion method and the juvenile fish culture method of oplegnathus fasciatus according to the present invention in detail with reference to specific embodiments, and the technical solutions of the present invention include, but are not limited to, the following embodiments.
Example 1
Selecting parent fish: the parent fish of oplegnathus fasciatus is required to be complete in body shape, normal in color, strong, harmless, active in movement, more than 2-year-old and more than 550g in body mass.
Temporary culture and cultivation ripening of parent fish: and (4) disinfecting the parent fish, putting the parent fish into a parent fish pond, and temporarily culturing for 1d by adopting an ecological simulation method (paying attention to the conditions of silence, darkness, deep water, water temperature, running water and the like). Parent fish density 3 fish/m3And 120 tails of parent fish groups. The seawater used in the parent fish pond for the first time is subjected to precipitation, sand filtration and disinfection by 10ppm bleaching powder for more than 24 hours in a special pond, and then is neutralized by sodium thiosulfate with the same concentration and aerated for about 4 hours, so that the environment is kept quiet. And feeding the oplegnathus fasciatus into the parent fish pond in the 2 nd day. The bait is palatable whole-grain oyster meat and palatable blocky frozen decapterus maruadsi, and the feeding amount is from small to large until the daily feeding amount is 5% of the weight of the fish. Change water and suck dirt 1 time each day. The feeding amount of the 1 st feeding is limited to 1 grain per tail, and then the feeding amount is increased day by day according to the ingestion condition. Generally, feeding in the morning and absorbing dirt in the evening, and changing water or adding 20% of water; changed inFresh seawater is also subjected to sand filtration, disinfection, chlorine elimination, aeration and other procedures. Proper amount of fish vitamins and fish antibiotics can be added into the bait. After 30d of indoor cement pond domestication, the parent fishes can swim in the same direction, when baiting is carried out, the parent fishes can float upwards and snatch, no dead fishes are recorded for a plurality of days continuously, and the breeding standard is met.
Micro-water flow production promotion: in the reproduction season of oplegnathus fasciatus in 5 months, the color change of sexually mature male and female fishes is observed, the body color of the common female fishes is relatively dark, the body fullness before spawning is increased, the gonad protrusion can be observed from the body surface, and if the sex glands are touched by hands, the sex glands are soft and elastic; the gonad of the male individual does not protrude, and the milky thick seminal fluid flows out when the male individual lightly presses the abdomen, is linear in water and quickly disperses. Stabilizing the water temperature at 23 ℃ for a plurality of days, arranging a plurality of low-power small water pumps on the bottom and the wall of the pool, forming middle and lower layer water level local jet flows under the continuous working condition, stimulating sexually mature parent fish of oplegnathus fasciatus to form mating behavior, and leading the parent fish to naturally ovulate.
Collecting and hatching fertilized eggs: the water inlet slowly feeds water to raise the water level in the parent fish pond, the floating eggs flow out from overflow holes of the pond, the overflow holes are supplemented with baffles to guide the floating eggs to overflow pipelines, and a water tank sleeved with a bolting silk net is used for collecting the floating eggs outside the overflow pipelines. The collected fertilized eggs are placed in a glass fiber reinforced plastic barrel, settled eggs (dead eggs) at the bottom of the barrel are sucked off through sedimentation, and floating eggs are collected at the rate of 0.5 ten thousand eggs/m3Is directly put in a density of more than 60m3Hatching in a cement pool of the water body. Every 2.0m21 air diffusing stone is arranged at the bottom of the pool in area, and the pool is continuously and slightly inflated and is periodically changed with water. Avoid the sudden change of environment and the direct irradiation of sunlight, and stop air regularly to suck the dead eggs and dirt at the bottom. And (5) checking the hatching condition of the fertilized eggs in the hatching process, and observing the development condition of the embryos.
Controlling the water quality: the water temperature is 23 ℃, the salinity is 28-29, and sudden change is avoided. The indoor illumination is sufficient and is adjusted according to the weather change, so that the illumination is adjusted to 1000-2000 Lux, and sudden change of the illumination and direct sunlight are avoided. Continuously inflating to ensure that the inflated bubbles are fine without dead corner areas, the inflating quantity is 0.1 to 0.5L/min before 10 days of age, and then 2 to 10L/min, and the water-soluble oxygen of the pond is more than 5 ml/L. The seawater is subjected to dark precipitation and sand filtration, and is filtered into a pool by using a 250-mesh bag, the pH value is 7.8-8.2, and the ammonia nitrogen value is below 0.3 mg/L.
The density of the sparus fasciatus larvae is as follows: the larval stage is about 0.5 ten thousand tails/m3(ii) a At the juvenile fish stage, about 0.3 ten thousand tails/m3(ii) a Young fish of over 45 days old is about 0.1 ten thousand tails/m3
Feeding management during the fry rearing period: according to the different requirements of the different development stages of the larvae and the young fishes on the nutrition and the palatability of the baits, different bait varieties are adopted to form a bait series for artificially culturing the larvae and the young fishes. The feeding key points of different baits are briefly described as follows:
brachionus plicatilis: feeding the chicken at the age of 2-10 days; artemia nauplii: feeding at the age of 11-20 days; live copepods and nauplii thereof: feeding at 9-30 days old. And controlling the feeding amount according to the age of the fry in days and the density.
Microcapsule feed: special microcapsule feed (feed diameter 0.2-0.5 micrometer) is fed in transition at 20 days old. The average sinking speed of the microcapsule feed in water is less than 0.1m/min, 5 percent of particles can be suspended for 2-3h, complete nutrition can be effectively supplemented in the process of fish larvae cultivation, copepods are replaced for feeding, the threat of exogenous diseases and drug residues brought by copepods to fry production is reduced, and the fry cultivation survival rate is improved. In order to domesticate young fishes and young fishes to eat, firstly feeding microcapsule feed every morning, and then feeding other baits; the feeding method is characterized in that the micro-capsule feed is fed in a small amount, multiple times and slowly, and the micro-capsule feed is fed in a water area with dense fry.
And (3) water flow management: the low-density cultivation of the large water body is kept, a water pump provided with a seedling suction prevention device is started to keep local underwater jet flow in the whole cultivation period, and the larval and young fishes are stimulated to be guided to flow upstream to form a plurality of cluster activity areas. Within 20 days of the young fishes, from 3 days of age, the water change amount is 5% every day, the dirt is absorbed for 1 time every week, the water change rate is 20% after 20 days of age, and the period of the young fishes is 30%. If the water quality is poor, intermittent running water can be considered.
Adding chlorella liquid and rotifer reinforcer: adding the chlorella liquid every day at regular time to keep the pond water at the concentration of 10-30 ten thousand/ml, showing a slight green color, reducing the ammonia nitrogen value and the transparency, applying a Hengxing brand rotifer enhancer (emulsion) before 30 days of age, and enhancing the nutrition of rotifers in the pond, thereby enhancing the physique of the larvae of the fish.
Separating the seedlings in a pond: when the larval fish have great size difference, the fry should be separated.
Seedling emergence: the average total length of the fry reaches more than 5.0cm, and the fry can be transferred to a marine net cage for culture by using a running water transportation device.
Example 2
Selecting parent fish: the parent fish of oplegnathus fasciatus is required to be complete in body shape, normal in color, strong, harmless, active in movement, more than 2-year-old and more than 550g in body mass.
Temporary culture and cultivation ripening of parent fish: and (4) disinfecting the parent fish, putting the parent fish into a parent fish pond, and temporarily culturing for 1d by adopting an ecological simulation method (paying attention to the conditions of silence, darkness, deep water, water temperature, running water and the like). Parent fish density 4 tails/m3The population remains > 160 tails. The seawater used in the parent fish pond for the first time is subjected to precipitation, sand filtration and disinfection by 10ppm bleaching powder for more than 24 hours in a special pond, and then is neutralized by sodium thiosulfate with the same concentration and aerated for about 4 hours, so that the environment is kept quiet. And feeding the oplegnathus fasciatus into the parent fish pond in the 2 nd day. The bait is palatable whole-grain oyster meat and palatable blocky frozen decapterus maruadsi, and the feeding amount is from small to large until the daily feeding amount is 5% of the weight of the fish. Change water and suck dirt 1 time each day. The feeding amount of the 1 st feeding is limited to 1 grain per tail, and then the feeding amount is increased day by day according to the ingestion condition. Generally, feeding in the morning and absorbing dirt in the evening, and changing water or adding 25% of water; the fresh seawater to be changed is also subjected to sand filtration, disinfection, chlorine elimination and aeration. Proper amount of fish vitamins and fish antibiotics can be added into the bait. After 30d of indoor cement pond domestication, the parent fishes can swim in the same direction, when baiting is carried out, the parent fishes can float upwards and snatch, no dead fishes are recorded for a plurality of days continuously, and the breeding standard is met.
Micro-water flow production promotion: observing the body color change of the sexually mature female and male oplegnathus fasciatus in month 4, wherein the body color of the common female and male oplegnathus fasciatus is relatively dark, the body fullness before spawning is increased, the gonad protrusion can be observed from the body surface, and if the male and female oplegnathus fasciatus is touched by hands, the male and female oplegnathus fasciatus has soft and elastic feeling; the gonad of the male individual does not protrude, and the milky thick seminal fluid flows out when the male individual lightly presses the abdomen, is linear in water and quickly disperses. Stabilizing the water temperature at 23 ℃ for a plurality of days, arranging a plurality of low-power small water pumps on the bottom and the wall of the pool, forming middle and lower layer water level local jet flows under the continuous working condition, stimulating sexually mature parent fish of oplegnathus fasciatus to form mating behavior, and leading the parent fish to naturally ovulate.
Collecting and hatching fertilized eggs: the water inlet slowly feeds water to raise the water level in the parent fish pond, the floating eggs flow out from overflow holes of the pond, the overflow holes are supplemented with baffles to guide the floating eggs to overflow pipelines, and a water tank sleeved with a bolting silk net is used for collecting the floating eggs outside the overflow pipelines. The collected fertilized eggs are placed in a glass fiber reinforced plastic barrel, settled eggs (dead eggs) at the bottom of the barrel are sucked off through sedimentation, and then floating eggs are collected at the rate of 0.7 ten thousand eggs/m3Is directly put in a density of more than 60m3Hatching in a cement pool of the water body. Every 1.5m21 air diffusing stone is arranged at the bottom of the pool in area, and the pool is continuously and slightly inflated and is periodically changed with water. Avoid the sudden change of environment and the direct irradiation of sunlight, and stop air regularly to suck the dead eggs and dirt at the bottom. And (5) checking the hatching condition of the fertilized eggs in the hatching process, and observing the development condition of the embryos.
Controlling the water quality: the water temperature is 23 ℃, the salinity is 28-29, and sudden change is avoided. The indoor illumination is sufficient and is adjusted according to the weather change, so that the illumination is adjusted to 1000-2000 Lux, and sudden change of the illumination and direct sunlight are avoided. Continuously inflating to ensure that the inflated bubbles are fine without dead corner areas, the inflating quantity is 0.1 to 0.5L/min before 10 days of age, and then 2 to 10L/min, and the water-soluble oxygen of the pond is more than 5 ml/L. The seawater is subjected to dark precipitation and sand filtration, and is filtered into a pool by using a 250-mesh bag, the pH value is 7.8-8.2, and the ammonia nitrogen value is below 0.3 mg/L.
The density of the sparus fasciatus larvae is as follows: the larval stage is about 0.7 ten thousand tails/m3(ii) a At the juvenile fish stage, about 0.4 ten thousand tails/m3(ii) a Young fish of over 45 days old is about 0.15 ten thousand tails/m3
Feeding management during the fry rearing period: according to the different requirements of the different development stages of the larvae and the young fishes on the nutrition and the palatability of the baits, different bait varieties are adopted to form a bait series for artificially culturing the larvae and the young fishes. The feeding key points of different baits are briefly described as follows:
brachionus plicatilis: feeding the chicken at the age of 2-10 days; artemia nauplii: feeding at the age of 11-20 days; live copepods and nauplii thereof: feeding at 9-30 days old. And controlling the feeding amount according to the age of the fry in days and the density.
Microcapsule feed: special microcapsule feed (feed diameter 0.2-0.5 micrometer) is fed in transition at 20 days old. The average sinking speed of the microcapsule feed in water is less than 0.1m/min, 5 percent of particles can be suspended for 2-3h, complete nutrition can be effectively supplemented in the process of fish larvae cultivation, copepods are replaced for feeding, the threat of exogenous diseases and drug residues brought by copepods to fry production is reduced, and the fry cultivation survival rate is improved. In order to domesticate young fishes and young fishes to eat, firstly feeding microcapsule feed every morning, and then feeding other baits; the feeding method is characterized in that the micro-capsule feed is fed in a small amount, multiple times and slowly, and the micro-capsule feed is fed in a water area with dense fry.
And (3) water flow management: the low-density cultivation of the large water body is kept, a water pump provided with a seedling suction prevention device is started to keep local underwater jet flow in the whole cultivation period, and the larval and young fishes are stimulated to be guided to flow upstream to form a plurality of cluster activity areas. Within 20 days of the young fishes, from 3 days of age, the water change amount is 5% every day, the dirt is absorbed for 1 time every week, the water change rate is 25% after 20 days of age, and the young fish period is 40%. If the water quality is poor, intermittent running water can be considered.
Adding chlorella liquid and rotifer reinforcer: adding the chlorella liquid every day at regular time to keep the pond water at the concentration of 10-30 ten thousand/ml, showing a slight green color, reducing the ammonia nitrogen value and the transparency, applying a Hengxing brand rotifer enhancer (emulsion) before 30 days of age, and enhancing the nutrition of rotifers in the pond, thereby enhancing the physique of the larvae of the fish.
Separating the seedlings in a pond: when the larval fish have great size difference, the fry should be separated.
Seedling emergence: the average total length of the fry reaches more than 5.0cm, and the fry can be transferred to a marine net cage for culture by using a running water transportation device.
Example 3
Selecting parent fish: the parent fish of oplegnathus fasciatus is required to be complete in body shape, normal in color, strong, harmless, active in movement, more than 2-year-old and more than 550g in body mass.
Temporary culture and cultivation ripening of parent fish: and (4) disinfecting the parent fish, putting the parent fish into a parent fish pond, and temporarily culturing for 1d by adopting an ecological simulation method (paying attention to the conditions of silence, darkness, deep water, water temperature, running water and the like). Parent fish density 5 fish/m3The population remained 200 tails. The seawater used in the parent fish pond for the first time is subjected to precipitation, sand filtration and disinfection by 10ppm bleaching powder for more than 24 hours in a special pond, and then is neutralized by sodium thiosulfate with the same concentration and aerated for about 4 hours, so that the environment is kept quiet. And feeding the oplegnathus fasciatus into the parent fish pond in the 2 nd day. The bait is palatable whole-grain oyster meat and palatable blocky frozen decapterus maruadsi, and the feeding amount is from small to large until the daily feeding amount is 5% of the weight of the fish. Change water and suck dirt 1 time each day. The feeding amount of the 1 st feeding is limited to 1 grain per tail, and then the feeding amount is increased day by day according to the ingestion condition. Generally, feeding in the morning and absorbing dirt in the evening, and changing water or adding 30% of water; the fresh seawater to be changed is also subjected to sand filtration, disinfection, chlorine elimination and aeration. Proper amount of fish vitamins and fish antibiotics can be added into the bait. After 30d of indoor cement pond domestication, the parent fishes can swim in the same direction, when baiting is carried out, the parent fishes can float upwards and snatch, no dead fishes are recorded for a plurality of days continuously, and the breeding standard is met.
Micro-water flow production promotion: in the reproduction season of oplegnathus fasciatus in 5 months, the color change of sexually mature male and female fishes is observed, the body color of the common female fishes is relatively dark, the body fullness before spawning is increased, the gonad protrusion can be observed from the body surface, and if the sex glands are touched by hands, the sex glands are soft and elastic; the gonad of the male individual does not protrude, and the milky thick seminal fluid flows out when the male individual lightly presses the abdomen, is linear in water and quickly disperses. Stabilizing the water temperature at 23-24 ℃ for a plurality of days, arranging a plurality of low-power small water pumps on the bottom and the wall of the pool, forming middle and lower layer water level local jet flows under the continuous working condition, stimulating sexually mature parent fishes of oplegnathus fasciatus to form mating behaviors, and enabling the parent fishes to naturally ovulate.
Collecting and hatching fertilized eggs: the water inlet slowly feeds water to raise the water level in the parent fish pond, the floating eggs flow out from overflow holes of the pond, the overflow holes are supplemented with baffles to guide the floating eggs to overflow pipelines, and a water tank sleeved with a bolting silk net is used for collecting the floating eggs outside the overflow pipelines. The collected fertilized eggs are placed in a glass fiber reinforced plastic barrel, settled eggs (dead eggs) at the bottom of the barrel are sucked off through sedimentation, and then floating eggs are collected at the rate of 1.0 ten thousand eggs/m3Is directly put in a density of more than 60m3Hatching in a cement pool of the water body. Every 1.5m21 air diffusing stone is arranged at the bottom of the pool with area, continuous micro-aeration and timing water change are carried out. Avoid the sudden change of environment and the direct irradiation of sunlight, and stop air regularly to suck the dead eggs and dirt at the bottom. And (5) checking the hatching condition of the fertilized eggs in the hatching process, and observing the development condition of the embryos.
Controlling the water quality: the water temperature is 23-24 ℃, the salinity is 28.5-30, and mutation is avoided. The indoor illumination is sufficient and is adjusted according to the weather change, so that the illumination is adjusted to 1000-2000 Lux, and sudden change of the illumination and direct sunlight are avoided. Continuously inflating to ensure that the inflated bubbles are fine without dead corner areas, the inflating quantity is 0.1 to 0.5L/min before 10 days of age, and then 2 to 10L/min, and the water-soluble oxygen of the pond is more than 5 ml/L. The seawater is subjected to dark precipitation and sand filtration, and is filtered into a pool by using a 250-mesh bag, the pH value is 7.8-8.2, and the ammonia nitrogen value is below 0.3 mg/L.
The density of the sparus fasciatus larvae is as follows: the larval stage is about 1.0 ten thousand tails/m3(ii) a At the juvenile fish stage, about 0.5 ten thousand tails/m3(ii) a Young fish of over 45 days old is about 0.2 ten thousand tails/m3
Feeding management during the fry rearing period: according to the different requirements of the different development stages of the larvae and the young fishes on the nutrition and the palatability of the baits, different bait varieties are adopted to form a bait series for artificially culturing the larvae and the young fishes. The feeding key points of different baits are briefly described as follows:
brachionus plicatilis: feeding the chicken at the age of 2-10 days; artemia nauplii: feeding at the age of 11-20 days; live copepods and nauplii thereof: feeding at 9-30 days old. And controlling the feeding amount according to the age of the fry in days and the density.
Microcapsule feed: special microcapsule feed (feed diameter 0.2-0.5 micrometer) is fed in transition at 20 days old. The average sinking speed of the microcapsule feed in water is less than 0.1m/min, 5 percent of particles can be suspended for 2-3h, complete nutrition can be effectively supplemented in the process of fish larvae cultivation, copepods are replaced for feeding, the threat of exogenous diseases and drug residues brought by copepods to fry production is reduced, and the fry cultivation survival rate is improved. In order to domesticate young fishes and young fishes to eat, firstly feeding microcapsule feed every morning, and then feeding other baits; the feeding method is characterized in that the micro-capsule feed is fed in a small amount, multiple times and slowly, and the micro-capsule feed is fed in a water area with dense fry.
And (3) water flow management: the low-density cultivation of the large water body is kept, a water pump provided with a seedling suction prevention device is started to keep local underwater jet flow in the whole cultivation period, and the larval and young fishes are stimulated to be guided to flow upstream to form a plurality of cluster activity areas. Within 20 days of the young fishes, from 3 days of age, the water change amount is 5% every day, the dirt is absorbed for 1 time every week, the water change rate is 30% after 20 days of age, and the young fish period is 50%. If the water quality is poor, intermittent running water can be considered.
Adding chlorella liquid and rotifer reinforcer: adding the chlorella liquid every day at regular time to keep the pond water at the concentration of 10-30 ten thousand/ml, showing a slight green color, reducing the ammonia nitrogen value and the transparency, applying a Hengxing brand rotifer enhancer (emulsion) before 30 days of age, and enhancing the nutrition of rotifers in the pond, thereby enhancing the physique of the larvae of the fish.
Separating the seedlings in a pond: when the larval fish have great size difference, the fry should be separated.
Seedling emergence: the average total length of the fry reaches more than 5.0cm, and the fry can be transferred to a marine net cage for culture by using a running water transportation device.
Comparative example 1
Micro-water flow production promoting technology: in 5 months of 2015, putting the sexually mature parent oplegnathus fasciatus into a 3-port 40-ton pond on average, feeding oysters for normal ripening promotion and cultivation, arranging a plurality of small water pumps at the bottom and on the pond wall of the No. 1 and No. 2 oplegnathus fasciatus parent fish pond during cultivation, forming middle and lower layer water level local jet flows in the pond, promoting and stimulating the oplegnathus fasciatus parent fish to perform spermiation and ovulation by water flow stimulation, realizing natural ovulation and insemination lasting for 16 days and 11 days for the two-port pond parent fish respectively, and collecting 2.7kg and 1.9kg of fertilized eggs; no. 3 parent fish pond of oplegnathus fasciatus is cultivated by adopting a traditional aeration mode, a small water pump is not installed for directional water flow stimulation, and only 0.23kg of fertilized eggs are collected every day in an accumulated mode.
Comparative example 2
Collecting and hatching fertilized eggs: 2015 + 2019, floating fertilized eggs of oplegnathus fasciatus are effectively collected in time in a mode of 'floating egg overflow-overflow hole baffle drainage-bolting silk net water tank' continuously; compared with 2014, the traditional manual net pulling mode is adopted, damage caused by extrusion and friction of net pulling operation on cell membranes of fertilized eggs is effectively reduced, mistaken fishing of unfertilized eggs (injecting: unfertilized eggs are generally suspended in water or sink at the bottom of a pool, fertilized eggs float on the water surface) is reduced, and the hatching rate is improved by more than 20%.
Comparative example 3
Feeding a microcapsule feed: in the juvenile stage of oplegnathus fasciatus, the micro-capsule feed is fed to reduce the threat of exogenous diseases and drug residues brought by copepods to fry production and improve the fry breeding survival rate. During the period of culturing 5-6 months of oplegnathus fasciatus fries in 2017, the juvenile oplegnathus fasciatus fed with the microcapsule feed has no bacterial/viral diseases in the whole process; when the sparus fasciatus seedlings of copepods are fed, 1 mouth of the sparus fasciatus seedlings has bacterial diseases, and the death rate is over 90 percent.
Comparative example 4
The directional flowing water cultivation method comprises the following steps: the low-density cultivation of a large water body is maintained, a water pump and a seedling suction prevention combination device are arranged, local underwater jet flow is maintained in the whole cultivation period, and the larval and young fish are stimulated to be guided to flow upstream to form a plurality of clusters. The results of 4 batches of seedling raising practices and seedling raising body length measurement in 2015-2019 show that the method effectively improves the artificial feeding efficiency and guarantees the nutritional requirements for the growth and development of the fries, the average body length of 90-day-old oplegnathus fasciatus fries is improved by more than 20% in 2015-2019 compared with that of 2014, and the survival rate of 1-90-day-old fries is improved to 35% from 2-6% in 2014. In addition, the directed flow cultivation method effectively exercises the fry traceability and the cluster awareness, can gather more than 95% of the fries, and after the fries are transferred into the offshore net cage for cultivation, the fries are strong in physique and vitality, strong in adaptability to the tide rising and falling in the sea area, and the fry loss rate in the temporary culture period (15 days) is reduced from 12% to more than 3%.
Comparative example 5
Adding chlorella liquid and rotifer reinforcer: in 2019, 4 months, a Hengxing rotifer enhancer (emulsion) is applied before 30 days of age, compared with the traditional enhancer, the water quality influence degree is small, the rotifer feeding property is strong, the nutritional ingredients of the rotifer are effectively enhanced, and the physique of young and young fishes is enhanced by feeding the rotifer.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.

Claims (10)

1. A flow-type production promoting method for oplegnathus fasciatus comprises the following steps:
1) putting the sterilized parent fish into a parent fish pond for temporary culture for 1d, feeding feed, changing water or adding water and absorbing dirt every day from the 2 nd day, and obtaining domesticated parent fish after 30-40 d;
2) in the reproduction period of the oplegnathus fasciatus, continuous local jet flow is provided for 15-20 days at the water level of the middle layer and the lower layer, so that mating of parent fish is completed, and the parent fish ovulates;
3) and (3) water is fed, so that the water level of the parent fish pond rises, the floating eggs flow out from the overflow holes of the parent fish pond, a baffle is arranged at the overflow hole to drain the floating eggs to an overflow pipeline, and the floating eggs are collected outside the overflow pipeline by using a water tank sleeved with a bolting silk net to obtain the oplegnathus fasciatus eggs.
2. The method according to claim 1, wherein the parent fish has a density of 3-5 fish/m3
3. The method for promoting the production according to claim 1, wherein the seawater used for the first time in the parent fish pond, the water for changing or adding water in the step 1) and the water for feeding in the step 3) independently comprise a pretreatment process; the pretreatment comprises precipitation, sand filtration, disinfection and chlorine-eliminating aeration.
4. The method for promoting production according to claim 1, wherein the amount of the water for changing or adding in the step 1) is 20-30% of the volume of the original hydrophilic pond water.
5. The method for cultivating the young sparus fasciatus in the juvenile fish stage based on the production promoting method of claims 1-4, comprising the following steps:
culturing the oplegnathus fasciatus eggs obtained by the spawning promotion method according to any one of claims 1 to 4, wherein in the whole culturing process, the water temperature is kept at 22-24 ℃, the salinity is 28-30, the pH value is 7.8-8.2, the ammonia nitrogen value is below 0.3mg/L, the light intensity is 1000-2000 Lux, chlorella vulgaris solution is added every day, the concentration of chlorella in water is 10-30 ten thousand/ml, the air is continuously inflated, and a water pump provided with a seedling absorption prevention device is started to keep local underwater jet flow;
feeding rotifer brachypomus at the age of 2-10 days, feeding artemia nauplii at the age of 11-20 days, feeding micro-capsule feed at the age of 20 days in a transitional mode, feeding living copepods and nauplii at the age of 9-30 days, and applying rotifer reinforcer before the age of 30 days.
6. The cultivation method as claimed in claim 5, wherein during the cultivation, the cultivation density is: larval stage of 0.5-1.0 ten thousand tails/m3(ii) a 0.3-0.5 ten thousand tails/m in the juvenile fish period3(ii) a Young fish of over 45 days old with 0.1-0.2 ten thousand tails/m3
7. The cultivation method as claimed in claim 5, wherein the continuous aeration is carried out at 0.1-0.5L/min before 10 days of age and at 2-10L/min thereafter.
8. The cultivation method according to claim 5, wherein the microcapsule feed has a diameter of 0.2 to 0.5 μm, an average sinking speed in water of < 0.1m/min, and 5% of particles can be suspended for 2 to 3 hours.
9. The cultivation method according to claim 5, wherein the cultivation is performed by changing the amount of water to 5% per day and sucking the soil 1 time per week from 3 days old within 20 days old; the water exchange amount is 20-30% after 20 days of age, and the water exchange amount is 30-50% after 45 days of age.
10. The cultivation method as claimed in claim 5, wherein the seedling absorption preventing means is a sphere with a diameter of 0.5 m and is densely covered with 80 mesh silk.
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