CN106688970A - Method for artificially breeding fingerlings of siganus vulpinus in indoor environments - Google Patents

Method for artificially breeding fingerlings of siganus vulpinus in indoor environments Download PDF

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Publication number
CN106688970A
CN106688970A CN201611021270.6A CN201611021270A CN106688970A CN 106688970 A CN106688970 A CN 106688970A CN 201611021270 A CN201611021270 A CN 201611021270A CN 106688970 A CN106688970 A CN 106688970A
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China
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fish
fox
pond
heat source
room heat
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CN201611021270.6A
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CN106688970B (en
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岳彦峰
彭士明
夏连军
张晨捷
邹雄
席寅峰
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East China Sea Fishery Research Institute Chinese Academy of Fishery Sciences
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East China Sea Fishery Research Institute Chinese Academy of Fishery Sciences
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish
    • Y02A40/818Alternative feeds for fish, e.g. in aquacultures

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Farming Of Fish And Shellfish (AREA)
  • Fodder In General (AREA)

Abstract

The invention relates to a method for artificially breeding fingerlings of marine ornamental fishes in indoor environments, particularly relates to a method for artificially breeding fingerlings of siganus vulpinus in indoor environments, and relates to the technical field of aquaculture. The method includes steps of selecting and breeding parent siganus vulpinus; carrying out hatching; breeding the fingerlings. The method has the advantages that the parent siganus vulpinus is preferentially selected wild parent siganus vulpinus, bait for parent siganus vulpinus breeding periods comprises high-quality vegetables, seaweeds and fish granulated feed, VC (vitamin C) and VE (vitamin E) are added into the bait in intensive breeding periods, accordingly, the quality of fertilized roe can be improved, excellent effects can be realized, and the fertilization rate and the hatching rate are higher than 80% under natural spawning conditions.

Description

A kind of method of fox basket fish room heat source
Technical field
The present invention relates to a kind of method of marine ornamental fish class room heat source, particularly a kind of interior of fox basket fish The technology of artificial breeding, is related to technical field of aquaculture.
Background technology
Fox basket fish (Siganus vulpinus) also known as fox fish, are a kind of heat of coral reef of Perciformes siganidae Band fish, is distributed in Philippine, South China Sea, Pacific coral reef marine site, and this fish body type outward appearance is flat in ellipse, It is small and sharp, it is quite long, protrude in a tubular form forward, body colour is golden yellow, body 15-20cm long, face floral designs exactly like fox head and Name, with ornamental value very high.
Because wild resource causes wild resource amount drastically to reduce by fisherman's overfishing in recent years, therefore of the invention have Effect is implemented to a certain extent solve the problems, such as fox basket fish resource amount, is also provided to protect or releasing this coral fishes A kind of practicable techniques.
The content of the invention
It is an object of the invention to provide a kind of method of fox basket fish room heat source, according to the skill that the present invention is provided Art scheme, the present invention will take the following:
S1, parent fish selection and cultivation:Body more than 18cm long in wild parent population is selected, surface is without wound, and vigor is good, health is disease-free Individuality as parent population, with freshwater soaking 10-15 minute after, be put into 27-31 DEG C of temperature, salinity is 25 ‰~32 ‰, and flow velocity is 50-80 liters/min, the depth of water is extremely laid eggs to be cultivated in the salt solution of 0.8-1.2m;The cultivation is divided into wild domestication and cultivates and strong Change and cultivate;The wild domestication about 5-7 days, feeds three times daily, interval is fed every time 4-6 hours, bait when starting to feed Using fresh food materials, fish treasured pellet is then gradually added into, until parent population ingests completely after fish treasured pellet, fed daily Bait be fish treasured pellet, fresh food materials fed once every 2-4 days;The reinforced cultivating, feeds twice daily, throws every time Feed interval 6-8 hour, bait formula major ingredient be fish treasured pellet, secondary major ingredient for shrimp and clam meat one or two, auxiliary material be Cod-liver oil, VC and spirulina powder one or more;The wild domestication cultivates the throwing amount with each bait of reinforced cultivating with 1 Eaten up in hour and bottom of pond noresidue is advisable.
S2, hatching;After by clean embryonated egg sterilization, hatching barrel hatching is moved into, be inflated to newly hatched larvae and black eye occur During point, transfer them to and cultivated in seed rearing pond;27-31 DEG C of the hatching barrel water temperature, salinity is 25 ‰~32 ‰;Institute State the thorough disinfection of seed rearing pond elder generation, then with after fresh water wash clean, injection seawater to 50-55cm, plus 1.5-2.5ppm prawn slice And the viable bacteria of 0.5-1ppm, while in pond plus 5-7*104Cell/ml micro-filtration ball algaes, maintain pool inner water color light green, transparent Degree 40cm or so, illumination 1000lx~10000lx.
S3, seed rearing:Before newly hatched larvae 15 days to feed based on wheel animalcule, wheel animalcule density 7-8/ml, splashed in pond The light and bacterium stoste of 0.5ppm or so;When newly hatched larvae grows into the 15th day, change throwing below 1mm small dimensions scratches sufficient class (example Such as wise water flea, angle water flea etc.), sufficient class density is scratched in pond and maintains 3-5/ml;When newly hatched larvae grows into 25 days, 1mm can be thrown Scratching for big specification scratches 2-4/ml of sufficient class density maintenance above in sufficient class (such as moina mongolica daday) pond;Treat that newly hatched larvae is developed into During juvenile fish, the meat gruel and fish treasured pellet of a diameter of 0.2-0.3mm are fed in right amount, cultivate interior 50-80 liters/min of flow velocity.
Wherein, in step S1 before cultivation, released in the salt solution and be put into the PVP-I of 0.4-0.6ppm and kill Bacterium is sterilized, and disinfects the time for 24 hours, after drug-treated is complete, does not change water, is directly cultivated.
Wherein, time of three times is fed daily for early at 7 points, 12 noon and late 17 points in step S1.
Wherein, it is early 7 points in the time for feeding daily twice of step S1,2 pm.
Wherein, one or more in the fresh food materials of step S1 are for romaine lettuce, Chinese cabbage, new fresh seaweed.
Wherein, step S1 reinforced cultivating bait formula by 60% fish treasured pellet, 20% shrimp, 10% clam Meat, 5% cod-liver oil, 2%VC, 1% nutrition fortifier and 2% spirulina powder composition
Wherein, the seed rearing pond thorough disinfection in step S1 is that the hypochlorous acid of 50ppm carries out disinfection.
Wherein, splash the VC of 1ppm in pond during the seed rearing of step S3.Be conducive to improving seed immunity and drop Low stress reaction.
Wherein, dipping and pharmaceutical chemistry are added in the cultivating process of step S1.Use what is relied mainly on prevention during parent fish rearing Healthy aquaculture mode, it then follows the use standard of China's drugs used in aquiculture, it should be noted that the prevention of disease.
Wherein, the dipping kills star 25ppm for grace promise, and Florfenicol 20ppm, the pharmaceutical chemistry kills star 1mg/kg and raises for grace promise Material, Florfenicol 1mg/kg feeds.
The measured fox basket fish parent fish of matter is cultivated, is the successful premise of artificial breeding, the quality of parent population can be directly affected to be received The acquisition of smart ovum and its quality.This fox basket fish parent fish cultivated is the wild parent population for preferentially selecting, during parent fish rearing Bait is vegetables, marine alga and the fish treasured pellet of high-quality, while adding VC and VE during reinforced cultivating to improve embryonated egg Quality, and achieve good effect.
Specific embodiment
With reference to embodiment, the invention will be further described:
Fish treasured pellet (Japanese woods and strain commercial firm, No. 7 material);Cod-liver oil (upper sea cowry inspires confidence in bio tech ltd); VC (Guangdong newly must biotech firm, 500g/ bags);Nutrition fortifier (Shandong Sheng Suo companies, 50DE- micro-capsules);Spirulina powder is (outstanding Precious Shui nationality articles for use company, 100g/ bags);Viable bacteria (Hainan brilliance biotech firm);Micro-filtration ball algae, (moral benefit abundance of water in Hainan produces company); Light and bacterium stoste (Zhengzhou photosynthetic organism Co., Ltd).
In June, 2016 to October implements fox basket in Donghai Aquatic Products Inst., China Aquatic Science Research Academy's Qionghai scientific base The room heat source of sub- fish.
1st, parent population source and reinforced cultivating
The wild parent population that parent population is fished for from Hainan Tan Men harbour purchase fishermen sea, selects body surface without wound, and vigor is good, The individuality that health is disease-free, parent population is transported to test center with running water car.Before parent population is put into cement pit, freshwater soaking 10-15 is used Minute.Seawater used by parent fish rearing enters pond through obscure shallow lake after sand filter filtering, salinity 25 ‰~32 ‰, cement pool water level is maintained The PVP-I sterilizing of 0.5ppm is put in 1m or so, pond.The time is disinfected for 24 hours, is not changed during drug-treated Water, flowing water is raised after treatment.50-80 liters/min of flow rate of water flow.
Just the parent population bought back starts to feed fresh vegetables (such as romaine lettuce, Chinese cabbage etc.), or new fresh seaweed for second day, often later It is progressively put into a small amount of fish treasured pellet (woods and feed), after parent population ingests fish treasured pellet completely, feeds daily Bait is mainly based on fish treasured pellet, and fresh vegetables fed once every 3 days.Feed bait to be eaten up in 1 hour every time And bottom of pond noresidue is advisable.Feed three times daily, early 7 points, 12 noon and late 17 points of each throwings are once.Bait during parent population reinforcing Material formula is the cod-liver oil+2%VC+1% nutrition fortifiers+2% of+10% clam meat of the 60% fish treasured shrimp of pellet+20%+5% Spirulina powder, early 7 points daily, 2 pm respectively throws once, is satiated with food and feeds.
2nd, culture pond prepares
By the fox basket fish of reinforced cultivating, sexual gland can be progressively ripe, and the second half of the month male and female parent population belly of the lunar calendar can be gradually Expand, egg-laying time is concentrated mainly on the lunar calendar 26 to 30.Spawning the last week prepares seed rearing pond (indoor culture Cement pit 3*4*1.2m, 2 mouthfuls of ponds), each culture pond first with hypochlorite solution's 50ppm thorough disinfection of valid density about 30%, Use fresh water wash clean again, be then injected into seawater 50cm, plus 2ppm prawn slice and the viable bacteria of 0.5-1ppm, while in pond plus few Amount micro-filtration ball algae, micro-filtration ball concentration of algae about 6*104Cell/ml, to protozoan in culture pond, pool inner water color maintains pale green Color, transparency 40cm or so, illumination 1000lx~10000lx.For the opening schistostoma size that sub- fish is incubated at the beginning of fox fish, spawning is worked as 200-300 mesh wheel animalcules, density 7-8/ml are put into Tianchi.
3rd, seed rearing
In the evening of the lunar calendar 26 to 30, when parent population starts to knock into the back mutually, start spawning, spawning is general 20:Opened after 30 Begin, ovum is viscosity, demersal egg.Morning collects embryonated egg, embryonated egg will wash clean repeatedly, and pulled out with the net of 40 mesh mixed The impurity in the middle of embryonated egg is closed, then the embryonated egg of wash clean is sterilized 10-15 minutes with the PVP-I of 0.1ppm, finally It is put into hatching in ready triangular barrel in advance, lasting inflation.28 degree of water temperature, under the conditions of salinity 28 ‰ or so, embryonated egg is through 25h Left and right can hatch rupture of membranes, newly hatched larvae total length 1.10-1.20mm, and aeration quantity turns (micro- inflation) down after membrane, just incubate son when see When black eyespot occurs in fish, transfer them to and cultivated in seed rearing pond.
Newly hatched larvae is put into Miao Chihou, in pond inflation be difficult it is too big, before newly hatched larvae 15 days to feed based on wheel animalcule, in pond Water colour keeps light green, wheel animalcule density 7-8/ml.Content of nitrite, content of nitrite control in pond in pond are surveyed in timing daily System is in below 0.3mg/l.Can splash the light and bacterium stoste of 0.5ppm or so in pond.When newly hatched larvae grows into the 15th day, change That throws below 1mm small dimensions scratches sufficient class (such as wise water flea, angle water flea etc.), scratches and sterilized with 0.5ppm PVP-Is before sufficient class feeds 30 minutes, sufficient class density is scratched in pond and maintains 3-5/ml.When newly hatched larvae grows into 25 days, the big specifications of more than 1mm can be thrown Sufficient class (such as moina mongolica daday) is scratched, sufficient class density is scratched in pond and is maintained 2-4/ml.When newly hatched larvae develops into juvenile fish, in right amount Meat gruel and fish treasured pellet are fed, it is fish treasured pellet of ingesting to gradate fox fish.Newly hatched larvae enters pond first day General to the tenth day not change water or change water on a small quantity, quantity of exchanged water is difficult more than 20%, can slowly be increased after the 15th day and be changed water To 30% or so, when Larval is into juvenile fish, quantity of exchanged water can increase to 50% or so to amount, water is slowly changed later and makes flowing water into Raise, 50-80 liters/min of flow velocity, bottom of pond rubbish will be suctioned out in time.Can be splashed in pond during seed rearing 1ppm VC to improve Seed immunity and reduction stress reaction.
4th, nurturing period disease control
Using the healthy aquaculture mode relied mainly on prevention during parent fish rearing, it then follows the use standard of China's drugs used in aquiculture, Note the prevention of disease, prophylactic agent is the conventional medicines such as Enrofloxacin HCL, Florfenicol, disinfectant.Precautionary measures are used (En Nuo kills star 1mg/kg feeds, and Florfenicol 1mg/kg is raised for dipping (En Nuo kills star 25ppm, Florfenicol 20ppm) and pharmaceutical chemistry Material) mode that is combined.
5th, result of implementation
The measured fox basket fish parent fish of matter is cultivated, is the successful premise of artificial breeding, the quality of parent population can be directly affected to be received The acquisition of smart ovum and its quality.This fox basket fish parent fish cultivated is the wild parent population for preferentially selecting, during parent fish rearing Bait is vegetables, marine alga and the fish treasured pellet of high-quality, while adding VC and VE during reinforced cultivating to improve embryonated egg Quality, and achieve good effect.Fertility rate and hatchability under the conditions of this natural spawning all more than 80%, but just Incubate larvae cultivation effect less desirable, may not enough be enriched with indoor cement pit open-mouthed bait, water environment destabilizing factor has Close.In June, 2016-October cultivates the tail of parent 80 altogether, survives 65 tails, tames success rate 81.25%, and embryonated egg about 21.3 is obtained altogether Wan Li, the tail of newly hatched larvae about 18.9 ten thousand, incubation rate reaches 88.7%, cultivates the tail of fox fish juvenile fish more than 1000.
The above is presently preferred embodiments of the present invention, but the present invention should not be limited to disclosed in the embodiment Content.So every do not depart from the lower equivalent or modification for completing of spirit disclosed in this invention, the model of present invention protection is both fallen within Enclose.

Claims (10)

1. a kind of method of fox basket fish room heat source, it is characterised in that methods described comprises the following steps:
S1, parent fish selection and cultivation:Body more than 18cm long in wild parent population is selected, surface is without wound, and vigor is good, what health was disease-free is individual Body as parent population, with freshwater soaking 10-15 minutes after, be put into 27-31 DEG C of temperature, salinity is 25 ‰~32 ‰, and flow velocity is 50-80 Liter/min, the depth of water is extremely laid eggs to be cultivated in the salt solution of 0.8-1.2m;The cultivation is divided into wild domestication initial stage and reinforced cultivating; The wild domestication about 5-7 days, feeds three times daily, interval is fed every time 4-6 hours, and bait uses fresh when starting to feed Food materials, are then gradually added into fish treasured pellet, until parent population ingests completely after fish treasured pellet, the bait for feeding daily is Fish treasured pellet, fresh food materials fed once every 2-4 days;The reinforced cultivating, is fed twice daily, and interval 6- is fed every time 8 hours, bait formula major ingredient be fish treasured pellet, secondary major ingredient be shrimp and clam meat one or two, auxiliary material be cod-liver oil, VC, nutrition fortifier and spirulina powder one or more;The throwing amount of each bait of the wild domestication and reinforced cultivating is with 1 Eaten up in hour and bottom of pond noresidue is advisable.
S2, hatching;After embryonated egg sterilization that will be clean, hatching barrel hatching is moved into, when being inflated to newly hatched larvae and black eyespot occur, Transfer them to and cultivated in seed rearing pond;The hatching barrel water temperature 27-31 degree, salinity 25 ‰~32 ‰;The seed The prawn slice of culture pond elder generation thorough disinfection, then with after fresh water wash clean, injection seawater to 50-55cm, plus 1.5-2.5ppm and The viable bacteria of 0.5-1ppm, while in pond plus 5-7*104Cell/ml micro-filtration ball algaes, maintain pool inner water color light green, transparency 40cm or so, illumination 1000lx~10000lx.
S3, seed rearing:To feed based on wheel animalcule, wheel animalcule density 7-8/ml, splash 0.5ppm in pond within 15 days before newly hatched larvae The light and bacterium stoste of left and right;When newly hatched larvae grows into the 15th day, change throwing below 1mm small dimensions scratches sufficient class, and foot is scratched in pond Class density maintains 3-5/ml;When newly hatched larvae grows into 25 days, can throw the big specifications of more than 1mm scratches sufficient class, and foot is scratched in pond Class density maintains 2-4/ml;When newly hatched larvae develops into juvenile fish, meat gruel and the fish that a diameter of 0.2-0.3mm is fed in right amount are precious Pellet, cultivates interior 50-80 liters/min of flow velocity.
2. the method for fox basket fish room heat source according to claim 1, it is characterised in that:In step S1 in training Before educating, the PVP-I sterilizing for being put into 0.4-0.6ppm is released in the salt solution, disinfects the time for 24 hours, After drug-treated is complete, water is not changed, directly cultivated.
3. the method for fox basket fish room heat source according to claim 1, it is characterised in that:In the daily of step S1 Time of three times is fed for early at 7 points, 12 noon and late at 17 points.
4. the method for fox basket fish room heat source according to claim 1, it is characterised in that:In the daily of step S1 The time for feeding twice is early 7 points, 2 pm.
5. the method for fox basket fish room heat source according to claim 1, it is characterised in that:In the fresh of step S1 Food materials are one or more in romaine lettuce, Chinese cabbage, new fresh seaweed.
6. the method for fox basket fish room heat source according to claim 1, it is characterised in that:In the reinforcing of step S1 The bait formula of cultivation is strong by 60% fish treasured pellet, 20% shrimp, 10% clam meat, 5% cod-liver oil, 2%VC, 1% nutrition Agent and 2% spirulina powder are constituted.
7. the method for fox basket fish room heat source according to claim 1, it is characterised in that:In the seed of step S1 Culture pond thorough disinfection is carried out disinfection with the hypochlorous acid of 50ppm.
8. the method for fox basket fish room heat source according to claim 1, it is characterised in that:In the seed of step S3 Splash the VC of 1ppm in nurturing period pond.
9. the method for fox basket fish room heat source according to claim 1, it is characterised in that:In the cultivation of step S1 During add dipping and pharmaceutical chemistry.
10. the method for fox basket fish room heat source according to claim 9, it is characterised in that:The dipping is grace Star 25ppm is killed in promise, and Florfenicol 20ppm, the pharmaceutical chemistry kills star 1mg/kg feeds, Florfenicol 1mg/kg feeds for grace promise.
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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108077116A (en) * 2017-12-08 2018-05-29 中国水产科学研究院东海水产研究所 A kind of artificial raise seedling method of wild six spots thorn Puffer
CN110100770A (en) * 2019-05-31 2019-08-09 海南晨海水产有限公司 A kind of artificial fecundation method of basket fish
CN111149735A (en) * 2020-01-21 2020-05-15 中国水产科学研究院南海水产研究所深圳试验基地 Large-scale cultivation method of siganus oramin fry
CN113317241A (en) * 2021-06-09 2021-08-31 中国水产科学研究院东海水产研究所 Method for gonadotropic development and artificial induced spawning of parent fish of takifugu hexamaculatus
CN113455428A (en) * 2021-08-17 2021-10-01 中国水产科学研究院东海水产研究所 Artificial breeding method for Mafu fish

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CN101703011A (en) * 2009-07-30 2010-05-12 浙江省海洋水产研究所 Method for cultivating synechogobius hast fry
CN102124974A (en) * 2011-03-15 2011-07-20 浙江省海洋水产研究所 Method for artificially breeding seed of spotted maigre
CN103503809A (en) * 2013-10-12 2014-01-15 浙江省舟山市水产研究所 Large yellow croaker artificial breeding method
CN103636543A (en) * 2013-12-18 2014-03-19 上海市水产研究所 Indoor artificial breeding method of glossogobius giuris

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US4913093A (en) * 1988-03-04 1990-04-03 Fresh-Culture Systems, Inc. Intensive aquaculture system
CN101703011A (en) * 2009-07-30 2010-05-12 浙江省海洋水产研究所 Method for cultivating synechogobius hast fry
CN102124974A (en) * 2011-03-15 2011-07-20 浙江省海洋水产研究所 Method for artificially breeding seed of spotted maigre
CN103503809A (en) * 2013-10-12 2014-01-15 浙江省舟山市水产研究所 Large yellow croaker artificial breeding method
CN103636543A (en) * 2013-12-18 2014-03-19 上海市水产研究所 Indoor artificial breeding method of glossogobius giuris

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108077116A (en) * 2017-12-08 2018-05-29 中国水产科学研究院东海水产研究所 A kind of artificial raise seedling method of wild six spots thorn Puffer
CN110100770A (en) * 2019-05-31 2019-08-09 海南晨海水产有限公司 A kind of artificial fecundation method of basket fish
CN110100770B (en) * 2019-05-31 2021-08-06 海南晨海水产有限公司 Artificial propagation method of siganus oramin
CN111149735A (en) * 2020-01-21 2020-05-15 中国水产科学研究院南海水产研究所深圳试验基地 Large-scale cultivation method of siganus oramin fry
CN111149735B (en) * 2020-01-21 2021-07-27 中国水产科学研究院南海水产研究所深圳试验基地 Large-scale cultivation method of siganus oramin fry
CN113317241A (en) * 2021-06-09 2021-08-31 中国水产科学研究院东海水产研究所 Method for gonadotropic development and artificial induced spawning of parent fish of takifugu hexamaculatus
CN113455428A (en) * 2021-08-17 2021-10-01 中国水产科学研究院东海水产研究所 Artificial breeding method for Mafu fish
CN113455428B (en) * 2021-08-17 2022-05-27 中国水产科学研究院东海水产研究所 Artificial breeding method for Mafu fish

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