CN108077116A - A kind of artificial raise seedling method of wild six spots thorn Puffer - Google Patents

A kind of artificial raise seedling method of wild six spots thorn Puffer Download PDF

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Publication number
CN108077116A
CN108077116A CN201711290691.3A CN201711290691A CN108077116A CN 108077116 A CN108077116 A CN 108077116A CN 201711290691 A CN201711290691 A CN 201711290691A CN 108077116 A CN108077116 A CN 108077116A
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wild
puffer
spots
water
feed
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岳彦峰
宋建强
彭士明
张晨捷
夏连军
高权新
施兆鸿
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East China Sea Fishery Research Institute Chinese Academy of Fishery Sciences
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East China Sea Fishery Research Institute Chinese Academy of Fishery Sciences
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • A01K61/10Culture of aquatic animals of fish
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Zoology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Farming Of Fish And Shellfish (AREA)

Abstract

The present invention relates to a kind of artificial raise seedling method of wild thorn Puffer, particularly a kind of artificial breeding technique of wild six spots thorn Puffer is related to technical field of aquaculture.The step of method provided by the invention, includes:(1) reinforced cultivating of parent population;(2) fertilized eggs are obtained and hatched;(3) seed rearing.The realization of the artificial breeding technique of the present invention, has filled up the blank of wild six spots thorn Puffer artificial breeding technology, while also pierces Puffer for six spot of Devoting Major Efforts To Developing from now on and provide technical support, has a extensive future.

Description

A kind of artificial raise seedling method of wild six spots thorn Puffer
Technical field
The present invention relates to a kind of artificial raise seedling method of wild thorn Puffer, particularly a kind of artificial breeding of wild six spots thorn Puffer Technology is related to technical field of aquaculture.
Background technology
Six spots pierce Puffer (Diodonholocanthus) as spoke fin net-rope, Tetraodontiformes, one kind of two tooth Molidaes, popular name gas melon Young, thorn rule etc..The maritime demersal fishes in the torrid zone mainly inhabit shallow sea reef area, soft bottom marine site or open waters.Generally Row solitary life is assembled in groups sometimes, and juvenile fish then float sexual life by row ocean.Six spots pierce Puffer delicious meat, and fish-skin is rich in rich Rich collagen, is received by the market, during highest up to 200-240 members/kilogram.Market potential is huge.Current six spot pierces Puffer The all marine fishings in source, stock number is also with fisherman's overfishing into the trend that falls sharply.
Six spots pierce Puffer as a kind of kind with important potentiality to be exploited, since its sources group quantity is unstable, have Apparent seasonality quantity of the catch, so total fishery harvesting yield is not high, also just supply falls short of demand in market.Excessive fishing causes six spots to pierce Puffer wild resource is destroyed, and catch can also be reduced therewith year by year.And it is propagated artificially and raising technology or blank at present.
The content of the invention
It is an object of the invention to provide a kind of wild six spots thorn artificial breeding techniques, fill up domestic aquatic products field wild six Spot pierces the blank of Puffer artificial breeding technology.The present invention is achieved by the following scheme:
A kind of artificial raise seedling method of wild six spots thorn Puffer, described method includes following steps:
(1) reinforced cultivating of parent population:By weight in more than 600g, long more than the 20cm of body, for body surface without wound, healthy vigor is good High-quality wild six spot thorn Puffer with plus 2ppm povidone iodine freshwater soaking 10min, place into flowing water and raise;The flowing water is 27.5-30 DEG C, pH 7.8~8.2, dissolved oxygen is kept>5mg/L.
Of fixed place and time daily feed twice, feeds bait as fresh and alive razor clam or clam class, is satiated with food and feeds, every afternoon into Row soil pick-up, 10 days a cycles, first 3 days of each cycle it is every 12 it is small when with the Enrofloxacin of 20ppm impregnate 12 it is small when after change water 100%;This reinforced cultivating has the following advantages:For the preferred fresh and alive clam or razor clam of wild six spot thorn Puffer natural habit, Enable the adaptation artificial domestication environment that wild parent population is faster and better, sexual gland maturation speed can than in natural marine site faster.
(2) fertilized eggs are obtained and hatched:The fertilized eggs for handling clean are put into seawater and are sterilized by parent population natural spawning, It pulls out after sterilization, is rinsed 1-2 times with fresh seawater, be then placed in hatchery hatching.The parent population stablized after artificial domestication can be The evening natural spawning of spawning season (the 5-9 months) early and middle ten days, the bolting silk net of secondary daily 100 mesh collect fertilized eggs;After collection Fertilized eggs with fresh seawater are cleaned up and remove impurity and dead ovum repeatedly;The fertilized eggs for handling clean are put into and have been added 0.5m3In the bucket of seawater, then add the sterilization of 1ppm formaldehyde 10 minutes, want continuous charge during sterilization in bucket;Fertilized eggs kill The bacterium time is pulled out in time with the fishing of the nets of 100 mesh when time is up, fertilized eggs pull out after again with clean seawater flushing 1-2 times, then gently It is lightly put into the hatchery (3*4*1.2m) being ready for, is hatched.
(3) seed rearing:Under the conditions of 29.0~31.0 DEG C of water temperature, salinity 30.0~32.0, incubating oosperm membrane is micro- Inflation, and cultivated according to the following steps;The VC that can be splashed in pond during seed rearing is improving seed immunity and reduction Stress reaction.
Feed within 3 days before S1 the wheel animalcule through fortification, artemia nauplii, the BP of 1ppm (with particulate manually raised by seedling Material), 1ppm vehicles member, the EM bacterium of the vc of 0.5ppm and 0.5ppm, and add in Nannochloropsis oculata in nursery pond, water colour made to keep pale green Color, content of nitrite are less than 0.3mg/L;
For S2 since the 4th day, feed below 1mm scratched sufficient class and artemia nauplii;
S3 fed the sufficient class of scratching of more than 1mm since the 10th day, fairy shrimp after fortification, the vc of 1ppm, 0.5ppm's EM bacterium;
S4 fed the nauplius of Penaeus Vannmei and the fairy shrimp of 2mm or so since the 15th day, the vc of 1ppm, The EM bacterium of 0.5ppm;
For S5 since the 20th day, feeding feed included the fairy shrimp of more than 5mm;
When S6 is into juvenile fish, the mixture of shrimp meat and fish treasured pellet is fed, slowly increases fish treasured particle in mixture Ratio, gradate juvenile fish for fish treasured pellet of ingesting;
After S7 40 days, the six spots thorn Puffer juvenile fish of 2.0-2.6cm bodies length is obtained;Juvenile period juvenile fish back is present with tool There are six big spots of obvious characteristic, and thorn can be hardened.
Wherein, in step (3), preceding 3 days quantity of exchanged water can be increased slowly after being no more than 20%, the 4th day in seed rearing For water to 50% or so, when Larval is into juvenile fish, quantity of exchanged water can increase to 80% or so, slowly change water later and flow instead Water is raised, and flow velocity 50-80L/min, bottom of pond rubbish will suction out in time.
Wherein, in step S1, the amount of the wheel animalcule of the fortification is 7-8/ml, and the amount of the artemia nauplii is 3-5/ml.
Wherein, in step S2, the amount for scratching sufficient class and artemia nauplii is 3-5/ml.
Wherein, in step S3, more than the 1mm's scratches sufficient class as moina mongolica daday, and throw-in density is 3-5/ml.
Wherein, in step S4, the throw-in density of the nauplius of the Penaeus Vannmei and the fairy shrimp of 2mm or so is 3-5/ml.
Wherein, in step S5, the Penaeus Vannmei shrimp seedling that feed further includes below 10mm is fed, the dispensing for feeding feed is close It spends for 0.5-1/ml.
The realization of the artificial breeding technique of the present invention has filled up the blank of wild six spots thorn Puffer artificial breeding technology, simultaneously Also pierce Puffer for six spot of Devoting Major Efforts To Developing from now on and technical support is provided, have a extensive future.
Description of the drawings
The speed of growth for the six spots thorn Puffer that Fig. 1 artificial raise seedling methods provided by the invention are cultivated.
Specific embodiment
With reference to embodiment, the invention will be further described:
It is further described with reference to example is applied.
In May, 2017 is to August in Hainan Province of Donghai Aquatic Products Inst., China Aquatic Science Research Academy Qionghai mayor slope town coconut palm The research center place of Lin Cun has carried out the artificial breeding research of wild six spots thorn Puffer parent population.
The reinforced cultivating of 1.1 parent populations
Parent population from the wild parent population of the marine fishing of Hainan Tan Men harbours purchase fisherman, select weight 600g with On, long more than the 20cm of body, body surface is without wound, the individual that health is disease-free.Parent population is transported with running water vehicle to test center.Just transport is returned Parent population will first with plus 2ppm povidone iodine freshwater soaking 10min, place into parent fish rearing pond and cultivated.In parent fish rearing Period, of fixed place and time daily feed twice, feed bait as fresh and alive razor clam or clam class, are satiated with food and feed, every afternoon carries out Soil pick-up.Every 10 days are a cycle, every 7 days with the Enrofloxacin of 20ppm impregnate 12 it is small when, 12 it is small when after change water 100%, even With the medicine of 3 days.Observation parent population active situation daily, parent fish rearing water temperature are 27.5-30 DEG C, pH 7.8~8.2, and dissolved oxygen is kept> 5mg/L。
1.2 fertilized eggs obtain and hatching
The parent population stablized after artificial domestication can be secondary daily in the evening natural spawning of spawning season (the 5-9 months) early and middle ten days The bolting silk net of 100 mesh collects fertilized eggs;By the fertilized eggs after collection cleaned up repeatedly with fresh seawater and remove impurity and Dead ovum;The fertilized eggs for handling clean are put into and have added 0.5m3In the bucket of seawater, then plus the sterilization 10 of 1ppm formaldehyde divides Clock wants continuous charge during sterilization in bucket;Fertilized eggs sterilizing time is pulled out in time with the net fishing of 100 mesh when time is up, fertilized eggs fishing It is then lightly put into the hatchery (3*4*1.2m) being ready for clean seawater flushing 1-2 times again after going out.Hatchery Ovum density is inside put as 5000/m3, hatching salinity 30-32 ‰, 29.0-31.0 DEG C of temperature, pH 7.8~8.2, dissolved oxygen holding> 5mg/L.Persistently inflated during incubating oosperm, do not change water, by 80h or so newly hatched larvaes successively rupture of membranes and go out.
1.3 seed rearing
Newly hatched larvae is put into Miao Chihou, is inflated in pond and is not easy too greatly, to feed the wheel animalcule through fortification within 3 days before newly hatched larvae (7-8/ml), artemia nauplii (3-5/ml), the BP of 1ppm, 1ppm vehicles member, while additionally added in seedling water body The EM bacterium of the vc of 0.5ppm, 0.5ppm, and Nannochloropsis oculata is added in nursery pond, water colour is made to keep light green, wheel animalcule density 7-8 A/ml, wherein artemia nauplii density 3-5, artemia nauplii are crucial open-mouthed bait, and nitrous in pond is surveyed in timing daily Phosphate content, content of nitrite control is in below 0.3mg/l in pond.When newly hatched larvae grows into the 4th day, change and throw below 1mm Small dimension scratches sufficient class, and artemia nauplii, the BP of 1ppm, 1ppm vehicles are first, and the EM bacterium of the vc of 0.5ppm, 0.5ppm scratch sufficient class It is sterilized 30 minutes with 0.5ppm povidone iodine before feeding, sufficient class is scratched in pond and artemia nauplii density maintains 3-5/ml.Just When incubating larvae growth by 10 days, can throw the big specifications of more than 1mm scratches sufficient class (such as moina mongolica daday), good year after fortification Worm, the EM bacterium of the vc of 1ppm, 0.5ppm, food density maintain 3-5/ml.It, can when newly hatched larvae development growth is to 15d Feed the fairy shrimp of the nauplius and 2mm or so for Penaeus Vannmei, the vc of density 3-5/ml, 1ppm, 0.5ppm's EM bacterium, when newly hatched larvae development growth is to 20d, can feed more than 5mm fairy shrimp and below 10mm South America it is right in vain Shrimp shrimp seedling, density 0.5-1/ml;During into juvenile fish, feed shrimp meat and fish treasured pellet in right amount, make six spots thorn Puffer juvenile fish by Gradually it is converted into fish treasured pellet of ingesting.Newly hatched larvae enters pond and does not generally change water or change water, quantity of exchanged water on a small quantity for first day to the 3rd day It is not easy more than 20%, quantity of exchanged water can be slowly increased to 50% or so, when Larval is into juvenile fish, quantity of exchanged water after the 4th day 80% or so can be increased to, water is slowly changed later and makes flowing water raising into, flow velocity 50-80L/min, bottom of pond rubbish will be inhaled in time Go out.The VC that can be splashed in pond during seed rearing to improve seed immunity and reduce stress reaction.
Influence of the 1 different openings bait of table to thorn Puffer prelarva (7d after hatching) survival rate
Note:Different Superscript letters represent there is significant difference (P<0.05).
Table 1 shows different openings bait larvae survival rate situation, in seedling pond 7-8/ml wheel animalcules density, 3-5/ml artemias without BP, 1ppm vehicle member processing group larvae survival rate of young density and 1ppm are saved as highest, is significantly higher than other processing groups.
2 vc of table handles the influence to piercing Puffer prelarva (9d after hatching) survival rate with EM bacterium
Note:* represent that conspicuousness is higher than control group (P<0.05).
Table 2 shows that normal processing group of cultivating adds vitamin C (vc), the situation of EM bacterium processing group larvae survival rates with additional. As can be seen from Table 2, the addition of vc and EM bacterium is remarkably improved the survival rate of prelarva.
The partial results of the young young young growth measurement of 3 six spot of table thorn Puffer
Newly hatched larvae is grown slower in preceding 15d it can be seen from table 3 and Fig. 1, and the speed of growth can be gradual after 15d Accelerate.
Nursery seawater meets China through sand filtration, water quality《Sea water quality standard》A kind of sea in (G B 30 97-1997) Water, hatching salinity 30-32 ‰, 29.0-31.0 DEG C of temperature, pH 7.8~8.2, dissolved oxygen are kept>5mg/L.0.5 every square metre is filled Gas head, intensity of illumination are controlled in 5000lx-10000lx.
Diet biomass in nursery pond is checked daily, observes the life habit of larval and juvenile fish, situation of ingesting and form Variation.
Conclusion:5~August in 2017 pierces Puffer to wild six spot by the method for the present invention and carries out artificial breeding research, in total Two batches juvenile fish is cultivated, altogether about 3000 tail juvenile fish, survival rate of seedling is 5%~6%, the batch phenomena of mortality does not during which occur.Cause This visible the method carries out nursery research to wild six spot thorn Puffer certain feasibility.
Example discussed above is only a kind of preferable operation scheme of the present invention, does not make limitation in any form, Those skilled in the art can also be made on the premise of without prejudice to the invention spirit a variety of equivalent modifications or It replaces, these equivalent modifications or replacement are all contained in the application claim limited range.

Claims (7)

1. a kind of artificial raise seedling method of wild six spots thorn Puffer, which is characterized in that described method includes following steps:
(1) reinforced cultivating of parent population:By weight in more than 600g, long more than the 20cm of body, body surface is without wound, good excellent of healthy vigor The wild six spots thorn Puffer freshwater soaking 10min for adding 2ppm povidone iodine of matter, places into flowing water and raises;The flowing water is 27.5-30 DEG C, pH 7.8~8.2, dissolved oxygen is kept>5mg/L;
Of fixed place and time daily feed twice, feeds bait as fresh and alive razor clam or clam class, is satiated with food and feeds, every afternoon soil pick-up, 10 days a cycles, first 3 days of each cycle it is every 12 it is small when with the Enrofloxacin of 20ppm impregnate 12 it is small when after change water 100%;
(2) fertilized eggs are obtained and hatched:The fertilized eggs for handling clean are put into seawater and are sterilized by parent population natural spawning, sterilization After pull out, with fresh seawater rinse 1-2 time, be then placed in hatchery hatch;
(3) seed rearing:Under the conditions of 29.0~31.0 DEG C of water temperature, salinity 30.0~32.0, incubating oosperm membrane is micro- to fill Gas, and cultivated according to the following steps;
Feed within 3 days before S1 the wheel animalcule through fortification, artemia nauplii, 1ppm BP, 1ppm vehicle member, 0.5ppm vc and The EM bacterium of 0.5ppm, and Nannochloropsis oculata is added in nursery pond, water colour is made to keep light green, content of nitrite is less than 0.3mg/ L;
For S2 since the 4th day, feed below 1mm scratched sufficient class and artemia nauplii;
S3 fed the sufficient class of scratching of more than 1mm, fairy shrimp after fortification, the EM of the vc of 1ppm, 0.5ppm since the 10th day Bacterium;
S4 fed the nauplius of Penaeus Vannmei and the fairy shrimp of 2mm or so since the 15th day, the vc of 1ppm, The EM bacterium of 0.5ppm;
For S5 since the 20th day, feeding feed included the fairy shrimp of more than 5mm;
When S6 is into juvenile fish, the mixture of shrimp meat and fish treasured pellet is fed, slowly increases the ratio of fish treasured particle in mixture Example, gradates juvenile fish for fish treasured pellet of ingesting;
After S7 40 days, the six spots thorn Puffer juvenile fish of 2.0-2.6cm bodies length is obtained.
2. the artificial raise seedling method of wild six spots thorn Puffer according to claim 1, which is characterized in that in step (3), seed Preceding 3 days quantity of exchanged water are no more than 20% in cultivation, and water can be slowly increased after the 4th day to 50% or so, treat Larval into During juvenile fish, quantity of exchanged water can increase to 80% or so, slowly change water later and make flowing water raising, flow velocity 50-80L/min, bottom of pond into Rubbish will suction out in time.
3. the artificial raise seedling method of wild six spots thorn Puffer according to claim 1, which is characterized in that described in step S1 The amount of the wheel animalcule of fortification is 7-8/ml, and the amount of the artemia nauplii is 3-5/ml.
4. the artificial raise seedling method of wild six spots thorn Puffer according to claim 1, which is characterized in that described in step S2 The amount of sufficient class and artemia nauplii is scratched as 3-5/ml.
5. the artificial raise seedling method of wild six spots thorn Puffer according to claim 1, which is characterized in that described in step S3 More than 1mm's scratches sufficient class as moina mongolica daday, and throw-in density is 3-5/ml.
6. the artificial raise seedling method of wild six spots thorn Puffer according to claim 1, which is characterized in that described in step S4 The throw-in density of the nauplius of Penaeus Vannmei and the fairy shrimp of 2mm or so is 3-5/ml.
7. the artificial raise seedling method of wild six spots thorn Puffer according to claim 1, which is characterized in that in step S5, feed Feed further includes the Penaeus Vannmei shrimp seedling of below 10mm, feeds the throw-in density of feed as 0.5-1/ml.
CN201711290691.3A 2017-12-08 2017-12-08 A kind of artificial raise seedling method of wild six spots thorn Puffer Pending CN108077116A (en)

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Cited By (4)

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Publication number Priority date Publication date Assignee Title
CN109511586A (en) * 2019-01-17 2019-03-26 中国水产科学研究院东海水产研究所 A kind of artificial cultivating method of six spots thorn Puffer seed
CN111316940A (en) * 2020-04-26 2020-06-23 海南晨海水产有限公司 Artificial breeding method of takifugu obscurus
CN112425535A (en) * 2020-12-21 2021-03-02 大连海洋大学 Method for accelerating early fry feeding habit conversion of takifugu rubripes
CN115886159A (en) * 2023-03-09 2023-04-04 海南热带海洋学院 Sexual gland development strengthening bait and method for hybridizing puffer fish with puffer fish

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109511586A (en) * 2019-01-17 2019-03-26 中国水产科学研究院东海水产研究所 A kind of artificial cultivating method of six spots thorn Puffer seed
CN111316940A (en) * 2020-04-26 2020-06-23 海南晨海水产有限公司 Artificial breeding method of takifugu obscurus
CN112425535A (en) * 2020-12-21 2021-03-02 大连海洋大学 Method for accelerating early fry feeding habit conversion of takifugu rubripes
CN115886159A (en) * 2023-03-09 2023-04-04 海南热带海洋学院 Sexual gland development strengthening bait and method for hybridizing puffer fish with puffer fish
CN115886159B (en) * 2023-03-09 2023-10-20 海南热带海洋学院 Gonad development strengthening bait and hybridization method for puffer fish and puffer fish hexaflumuron

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