CN109511586A - A kind of artificial cultivating method of six spots thorn Puffer seed - Google Patents

A kind of artificial cultivating method of six spots thorn Puffer seed Download PDF

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Publication number
CN109511586A
CN109511586A CN201910042499.5A CN201910042499A CN109511586A CN 109511586 A CN109511586 A CN 109511586A CN 201910042499 A CN201910042499 A CN 201910042499A CN 109511586 A CN109511586 A CN 109511586A
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CN
China
Prior art keywords
seed
feeds
pond
shrimp
bait
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Pending
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CN201910042499.5A
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Chinese (zh)
Inventor
岳彦峰
房文红
夏连军
彭士明
高权新
王孝杉
邹雄
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East China Sea Fishery Research Institute Chinese Academy of Fishery Sciences
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East China Sea Fishery Research Institute Chinese Academy of Fishery Sciences
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Application filed by East China Sea Fishery Research Institute Chinese Academy of Fishery Sciences filed Critical East China Sea Fishery Research Institute Chinese Academy of Fishery Sciences
Priority to CN201910042499.5A priority Critical patent/CN109511586A/en
Publication of CN109511586A publication Critical patent/CN109511586A/en
Pending legal-status Critical Current

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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00Culture of aquatic animals
    • A01K61/10Culture of aquatic animals of fish
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/20Animal feeding-stuffs from material of animal origin
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/20Animal feeding-stuffs from material of animal origin
    • A23K10/22Animal feeding-stuffs from material of animal origin from fish
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/174Vitamins
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/80Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

Abstract

The invention discloses a kind of artificial cultivating methods of six spots thorn Puffer seed, including aquaculture pond prepares, seed is screened, domestication and later period cultivation, 4~23d feed domestication and daily soil pick-up by 3% that feeding volume is total juvenile fish weight;Wherein, 4~6d feeds the big fairy shrimp of 80% reinforcing, 19% shrimp meat and 1% vitamin C, 7~9d feeds big fairy shrimp, 49% shrimp meat and 1% vitamin C of 50% reinforcing, 10~12d feeds 20% big fairy shrimp, 60% shrimp meat and 20% fish treasured pellet, 13~15d feeds 60% shrimp meat and 40% fish treasured pellet, 16~19d feeds 40% shrimp meat and 60% fish treasured pellet, and 20~23d feeds 20% shrimp meat and 80% fish treasured pellet;Direct labor cultivates after 24d, is satiated with food and feeds.Above-mentioned artificial cultivating method fills up the blank of domestic six spots thorn Puffer fingerlings artificial cultural technique.

Description

A kind of artificial cultivating method of six spots thorn Puffer seed
Technical field
The present invention relates to a kind of artificial cultivating method for piercing Puffer, especially a kind of artificial breeding skill of six spots thorn Puffer seed Art is related to technical field of aquaculture.
Background technique
It is spoke fin net-rope that six spots, which pierce Puffer (Diodonholocanthus), and Tetraodontiformes, one kind of two tooth Molidaes is tropic sea Property demersal fishes in ocean mainly inhabit shallow sea reef area, soft bottom sea area or open waters.Six spots pierce Puffer delicious meat, fish Skin is rich in collagen abundant, is received by the market, and current six spot pierces all sea fishings in Puffer source, and stock number is with fishing People's overfishing is at the trend that falls sharply.Since the sources group quantity of six spots thorn Puffer is unstable, there is apparent seasonal quantity of the catch, Total fishery harvesting yield is not high, and also just supply falls short of demand in market, its present artificial breeding technology has been successful, but later period seed The current still blank of cultural technique.
Summary of the invention
The purpose of the present invention is to provide a kind of artificial cultivating methods of six spots thorn Puffer seed, fill up domestic aquatic products field six Spot pierces Puffer fingerlings artificial cultural technique blank.
Above-mentioned purpose of the invention is achieved through the following technical solutions:
Six spots pierce the artificial cultivating method of Puffer seed, specifically, comprising the following steps:
(1) aquaculture pond prepare: by aquaculture pond and utensil all with bleach water sterilization after clean up, and the aquaculture pond with The utensil corresponds to each other, and does not use with;The utensil includes dirt sucking device, brush and pneumatic head;Prepare breeding water simultaneously, In, by nursery seawater, the water quality after sand filtration meets a kind of sea in " sea water quality standard " (GB3097-1997) to the breeding water Water and obtain, salinity be 20 ‰~32 ‰, temperature be 23~31 DEG C, pH be 7.2~8.5, dissolved oxygen holding > 5mg/L, flowing water;
(2) seed is screened: selecting six spots thorn Puffer seed, a length of 2~4cm of body, wherein six spots of a length of 2~3cm of body pierce Puffer Seed is 100 tails/m according to density3Enter pond, the six spots thorn Puffer seed of a length of 3~4cm of body is 80 tails/m according to density3Enter pond, and The seed adds 0.1ppm Betagen Solution on the day of entering pond, change water completely after 12h and 3d is used in conjunction;
(3) seed is tamed: just enter six spot of pond 1d thorn Puffer seed and has not fed, 2d starts to feed bait after stablizing, then It is tamed by way of periodically changing and feeding bait;
(4) the seed later period cultivates: every morning and afternoon respectively feed a bait, are satiated with food and feed, and thoroughly brush is primary every other day Aquaculture pond bottom;During cultivation at regular intervals, primary by the speed of growth of juvenile fish and physique sieve fish, size is separated;Every month Pond is primary, and 2 days before pond, 2ppm vitamin C of splashing in breeding water body is finished down behind pond first 2 days, 2ppm grace of splashing in water body Promise Sha Xing;Every 15d, to change 0.5ppm copper sulphate of splashing after water into aquaculture pond afternoon, do not change water at night, next day changes water 100%, 3d is used in conjunction.
Further, in step (1), the specification of the aquaculture pond is 3m × 4m × 1.2m, and each aquaculture pond is uniformly put Set 6~10 pneumatic heads.
Further, in step (3), the domestication process are as follows:
It feeds within 3 days before (3-1) seed and strengthens fairy shrimp, every morning, afternoon be of fixed place and time to be fed 2 times, is satiated with food and is fed, throws Not flowing water during feeding, the flowing water after seed eats up;Wherein, the strengthening process for strengthening fairy shrimp are as follows: temporarily bringing fairy shrimp up Water body in 2ppmVC, 10ppm spirulina powder be added strengthen 2h, before feeding again plus 1ppm Betagen Solution disinfection 1h, then use It is fed after seawater flushing is clean;
(3-2) 4~23d feeds the seed according to 3% that feeding volume is overall juvenile fish weight, and daily Dusk soil pick-up;Wherein, the bait group that feeds of 4~6d strengthens big fairy shrimp, 19% shrimp meat and 1% vitamin C as 80% Mixed feed;7~9d feed bait group as 50% strengthen big fairy shrimp, 49% shrimp meat and 1% ascorbic Mixed feed;10~12d's feeds bait group as 20% big fairy shrimp, 60% shrimp meat and 20% fish treasured pellet Mixed feed;The bait group that feeds of 13~15d becomes the mixed feed of 60% shrimp meat and 40% fish treasured pellet;16th The bait group that feeds of~19d becomes the mixed feed of 40% shrimp meat and 60% fish treasured pellet;20~23d's feeds bait Material group becomes the mixed feed of 20% shrimp meat and 80% fish treasured pellet;
After (3-3) 24d, six spots pierce Puffer seed and adapt to fish treasured pellet completely, directly feed hand fit's feeding Material, is satiated with food and feeds, respectively throw in the morning, afternoon once, daily dusk soil pick-up.
Further, the artificial cultivating method of the six spots thorn Puffer seed further include:
Diet biomass in aquaculture pond, measurement water Inversion phenomenon and pH are checked daily;
Observe juvenile fish life habit, situation of ingesting and metamorphosis;
Periodic measurement juvenile fish weight and body are long.
The beneficial effects of the present invention are: the six spots thorn above-mentioned artificial cultivating method of Puffer seed is simple, easily operated, cost It is low, fill up the blank of domestic six spot of aquatic products field thorn Puffer fingerlings artificial cultural technique.
Specific embodiment
It is further described below with reference to example is applied.
In May, 2018 is to August in Hainan Province, Donghai Aquatic Products Inst., China Aquatic Science Research Academy Qionghai mayor slope town coconut palm The research center place of Lin Cun carries out the thorn Puffer fingerlings artificial cultivation of six spots, and six spots thorn Puffer juvenile fish used is that inventor passes through people Six spots that work breeding obtains pierce Puffer seed, carry out six spots according still further to following cultural method and pierce the nursery of Puffer seed.
1, aquaculture pond prepares
Aquaculture pond specification used is 3m × 4m × 1.2m, and aquaculture pond and utensil used all use bleaching water before putting seedling Clean up spare after disinfection, the utensils such as soil pick-up and brush that each aquaculture pond is equipped with correspond to respective pond, cannot use with;Each The pneumatic head of aquaculture pond uniformly places 8.
Water quality requirement: through sand filtration, water quality meets a kind of in China " sea water quality standard " (GB3097-1997) nursery seawater Seawater, salinity 20~32 ‰, 23~31 DEG C of temperature, pH7.2~8.5, dissolved oxygen holding > 5mg/L, flowing water.
2, seed size screens
Seed is screened before entering pond by size, and the pond of putting that specification is 2~3cm, density is 100 tails/m3, 3~4cm's puts One pond, 80 tails of density/m3.Seed adds 0.1ppm Betagen Solution on the day of entering pond, change water 100% after 12h, 3d is used in conjunction.
3, seed is tamed
The six spots thorn Puffer seed for just having entered pond 1d does not feed, and the 2d after stablizing starts to feed bait, tames the side of feeding Method carries out in the following manner:
1~3d, six spots pierce Puffer juvenile fish in the big fairy shrimp for feeding reinforcing for first 3 days for entering pond, and are temporarily bringing the good year up 2ppmVC, 10ppm spirulina powder are added in the water body of worm, carries out reinforcing 2h, before feeding again in the water body for temporarily bringing fairy shrimp up Add 1ppm Betagen Solution to sterilize 1h, then pull the big fairy shrimp after strengthening out and is completely fed again with seawater flushing.Daily The morning, afternoon be of fixed place and time to be fed 2 times, is satiated with food and is fed, not flowing water during feeding, the flowing water after juvenile fish eats up.
4~6d feeds the ascorbic mixing bait of+19% shrimp meat of big fairy shrimp+1% that bait changes 80% reinforcing into Material, feeding volume fed by the 3% of overall juvenile fish weight, and siphons away after 2h the bait that bottom of pond is not eaten up.
7~9d, feeding bait is 50%+49% shrimp meat of big fairy shrimp strengthened ++ 1% ascorbic mixing bait Material, feeding volume is fed by the 3% of overall juvenile fish weight, and after 2h, siphons away the bait that bottom of pond is not eaten up.
10~12d feeds bait group as 20% big+20% fish treasured pellet of+60% shrimp meat of fairy shrimp, feeds Amount is fed by the 3% of overall juvenile fish weight, and after 2h, siphons away the bait that bottom of pond is not eaten up.
13~15d feeds bait group as+40% fish treasured pellet of 60% shrimp meat, and feeding volume presses overall juvenile fish The 3% of weight is fed, and after 2h, siphons away the bait that bottom of pond is not eaten up.
16~19d feeds bait group as+60% fish treasured pellet of 40% shrimp meat, and feeding volume presses overall juvenile fish The 3% of weight is fed, and after 2h, siphons away the bait that bottom of pond is not eaten up.
20~23d feeds bait group as+80% fish treasured pellet of 20% shrimp meat, and feeding volume presses overall juvenile fish The 3% of weight is fed, and after 2h, siphons away the bait that bottom of pond is not eaten up.
After 24d, juvenile fish just directly feeds artifical compound feed after adapting to fish treasured pellet completely, and feeding volume is pressed The 3% of overall juvenile fish weight is fed, and after 2h, siphons away the bait that bottom of pond is not eaten up.
Setting directly feeds the contrast groups (A group) of pellet simultaneously, and above-mentioned domesticating method is set as experimental group (B group), 180 tail of juvenile fish of uniform size is selected, 90 tail of A group, 90 tail of B group, every group three parallel, each parallel 30 tail fish, counts after 25d Survival rate, and SPSS13 statistical software analyzes data, p < 0.05 is considered as that there were significant differences, and test result is as shown in table 1.
Table 1
Note: a, b indicate significant difference between different groups.
As shown in Table 1, it after bait converts 15d, is raised and train after feeding method is remarkably improved according to juvenile fish of the invention Six spot of phase pierces the cultivation survival rate of Puffer juvenile fish, is also significantly greater than contrast groups (A group) in growth indexes body long side.
4, the seed later period cultivates
Every morning, afternoon be of fixed place and time respectively to feed a bait, is satiated with food and feeds, every other day a thorough aquaculture pond bottom of brush; Every 20d during cultivation, sieve fish is carried out by the size of juvenile growth at this time, the fish of unified specification is put together by rational density, Sieve fish the previous day not bait throwing in material;Fall that pond is primary, and 2 days before pond, 2ppm vitamin C of splashing in breeding water body is to improve every 30d Its stress ability is finished down behind pond first 2 days, 2ppm Enrofloxacin of splashing in water body prevention infection;Every 15d, after change water in the afternoon It splashes into aquaculture pond 0.5ppm copper sulphate, prevents helminth, do not change water at night, next day changes water 100%, 3d is used in conjunction.
In addition, checking diet biomass in aquaculture pond, measurement water Inversion phenomenon and pH daily in entire breeding process, seeing Juvenile fish life habit is examined, situation of ingesting and metamorphosis, periodic measurement juvenile fish weight and body are long.
Example described above is only the preferable operation scheme of the present invention, does not make any form of restriction, is familiar with ability The technical staff in domain can also make various equivalent variation or replacement on the premise of not violating the inventive spirit of the present invention, these Equivalent variation or replacement are all included in the scope defined by the claims of the present application.

Claims (4)

1. a kind of artificial cultivating method of six spots thorn Puffer seed, which comprises the following steps:
(1) aquaculture pond prepares: will clean up after aquaculture pond and disinfecting utensils, and the aquaculture pond and the utensil are mutually right It answers, does not use with, wherein the utensil includes dirt sucking device, brush and pneumatic head;Prepare breeding water simultaneously, by nursery seawater Water quality meets GB3097-1997 one kind seawater and obtains after sand filtration, and salinity is 20 ‰~32 ‰, and temperature is 23~31 DEG C, and pH is 7.2~8.5, dissolved oxygen holding > 5mg/L, flowing water;
(2) seed is screened: selecting six spots thorn Puffer seed, a length of 2~4cm of body, wherein six spots of a length of 2~3cm of body pierce Puffer seed It is 100 tails/m by density3Enter pond, the six spots thorn Puffer seed of a length of 3~4cm of body is 80 tails/m by density3Enter pond, the seed enters Add 0.1ppm Betagen Solution on the day of pond, changes water completely after 12h and 3d is used in conjunction;
(3) seed is tamed: just having entered six spot of pond 1d thorn Puffer seed and has not fed, 2d starts to feed bait after stablizing, then passes through The mode that periodically change feeds bait is tamed;
(4) the seed later period cultivates: every morning and afternoon are respectively satiated with food and feed once, thoroughly brush an aquaculture pond bottom every other day;Cultivation Period is separated by the speed of growth and body long periodically sieve fish, the size of juvenile fish;It is primary to fall pond every month, and 2 days cultivation waters before pond It splashes in body 2ppm vitamin C, is finished down behind pond 2ppm Enrofloxacin of splashing in preceding 2 days bodies;Every 15d, change afternoon after water to supporting 0.5ppm copper sulphate of splashing in pond is grown, does not change water at night, next day changes water 100%, 3d is used in conjunction.
2. the artificial cultivating method of six spots thorn Puffer seed according to claim 1, which is characterized in that described to support in step (1) The specification for growing pond is 3m × 4m × 1.2m, and each aquaculture pond uniformly places 6~10 pneumatic heads.
3. the artificial cultivating method of six spots thorn Puffer seed according to claim 1, which is characterized in that described to tame and docile in step (3) Change process are as follows:
It feeds within 3 days before (3-1) seed and strengthens fairy shrimp, every morning, of fixed place and time be satiated with food in afternoon feed 2 times, and feed period Not flowing water, the flowing water after seed eats up;Wherein, the strengthening process for strengthening fairy shrimp are as follows: in the water body for temporarily bringing fairy shrimp up Middle addition 2ppmVC, 10ppm spirulina powder strengthens 2h, and before feeding plus 1ppm Betagen Solution sterilizes 1h, then uses seawater flushing It is fed after clean;
(3-2) 4~23d is fed the seed by 3% that feeding volume is overall juvenile fish weight, and inhaled at dusk daily It is dirty;Wherein, the bait group that feeds of 4~6d strengthens big fairy shrimp, 19% shrimp meat and 1% ascorbic mixing as 80% Bait;The bait group that feeds of 7~9d becomes 50% big fairy shrimp, 49% shrimp meat and the 1% ascorbic mixing bait strengthened Material;10~12d's feeds bait group as 20% big fairy shrimp, the mixing bait of 60% shrimp meat and 20% fish treasured pellet Material;The bait group that feeds of 13~15d becomes the mixed feed of 60% shrimp meat and 40% fish treasured pellet;16~19d Feed bait group become 40% shrimp meat and 60% fish treasured pellet mixed feed;20~23d's feeds bait group Mixed feed as 20% shrimp meat and 80% fish treasured pellet;
After (3-3) 24d, six spots thorn Puffer seed, which is directly satiated with food, feeds artifical compound feed, respectively throws in the morning, afternoon once, and Daily dusk soil pick-up.
4. the artificial cultivating method of six spots thorn Puffer seed according to claim 1, which is characterized in that six spot pierces Puffer seed Artificial cultivating method further include:
Diet biomass in aquaculture pond, measurement water Inversion phenomenon and pH are checked daily;
Observe juvenile fish life habit, situation of ingesting and metamorphosis;
Periodic measurement juvenile fish weight and body are long.
CN201910042499.5A 2019-01-17 2019-01-17 A kind of artificial cultivating method of six spots thorn Puffer seed Pending CN109511586A (en)

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101281396B1 (en) * 2012-11-06 2013-07-02 충청남도 Spawning induction by change of environment for takifugu
CN107667916A (en) * 2017-08-29 2018-02-09 天津农学院 A kind of young hippocampus uses the method for culturing seedlings for freezing bait
CN108077116A (en) * 2017-12-08 2018-05-29 中国水产科学研究院东海水产研究所 A kind of artificial raise seedling method of wild six spots thorn Puffer
CN108077122A (en) * 2017-12-25 2018-05-29 中国水产科学研究院东海水产研究所 A kind of artificial acclimation method of wild lead point Fugu

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101281396B1 (en) * 2012-11-06 2013-07-02 충청남도 Spawning induction by change of environment for takifugu
CN107667916A (en) * 2017-08-29 2018-02-09 天津农学院 A kind of young hippocampus uses the method for culturing seedlings for freezing bait
CN108077116A (en) * 2017-12-08 2018-05-29 中国水产科学研究院东海水产研究所 A kind of artificial raise seedling method of wild six spots thorn Puffer
CN108077122A (en) * 2017-12-25 2018-05-29 中国水产科学研究院东海水产研究所 A kind of artificial acclimation method of wild lead point Fugu

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
胡亚丽 等: "暗纹东方鲀幼鱼发育及工厂化育苗的研究", 《水利渔业》 *

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