CN107114283A - A kind of artificial breeding method of Collichthys lucidus - Google Patents

A kind of artificial breeding method of Collichthys lucidus Download PDF

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CN107114283A
CN107114283A CN201710492929.4A CN201710492929A CN107114283A CN 107114283 A CN107114283 A CN 107114283A CN 201710492929 A CN201710492929 A CN 201710492929A CN 107114283 A CN107114283 A CN 107114283A
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water
collichthys lucidus
collichthys
lucidus
seminal fluid
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CN107114283B (en
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叶坤
王志勇
陈庆凯
胡国良
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Jimei University
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00—Culture of aquatic animals
    • A01K61/10—Culture of aquatic animals of fish
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/20—Animal feeding-stuffs from material of animal origin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81—Aquaculture, e.g. of fish

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Abstract

The invention discloses a kind of artificial breeding method of Collichthys lucidus, belong to fish reproduction field, its step includes：Adult fish is fished for as parent, artificial insemination and fry rearing.Extraction and diluted concentration of the present invention by Collichthys lucidus seminal fluid, the ratio and time of insemination of smart ovum mixing, the rate of fertilization, incubation rate and survival rate of fish fry of Collichthys lucidus are improved, is that the full artificial breeding of Collichthys lucidus lays the foundation, is that Collichthys lucidus propagates the enough seeds of offer artificially.

Description

A kind of artificial breeding method of Collichthys lucidus
Technical field
The invention belongs to fish reproduction field, and in particular to a kind of artificial breeding method of Collichthys lucidus.
Background technology
Collichthys lucidus (Collichthys lucidus) category Perciformes, Sciaenidae, Collichthy is China coastal seas The common small-sized economic fish of dwelling property of bottom, is mainly distributed on the East Sea and the Huanghai Sea, to the east of marine products amount it is maximum.Collichthys lucidus is short distance From the shallow sea fish of migration, happiness is perched in river mouth salt-fresh water intersection, fast growth, and thermophilic, proper salinity level are wide, and its meat is thin It is tender and delicious, it is one of high-grade aquatic products of coastal resident's eating.In recent years, due to the original such as overfishing, offshore pollution be serious Cause, causes the natural resources amount of Collichthys lucidus to be greatly decreased, the demand of its artificial propagation techniques and cultural technique is arisen at the historic moment, But a whole set of effective Collichthys lucidus artificial breeding technology is not formed yet at present, it is impossible to realize the quantization life of artificial culture fry Production, it is propagated industry artificially can not also develop.The main bottleneck of Collichthys lucidus artificial breeding has, and one is of Collichthys lucidus Body is smaller, and female fish brood amount is not high, and male semen volume is less, and male and female fish gonad development is asynchronous so that it is a large amount of obtain by Smart ovum is extremely difficult；Two be the fertility rate and hatchability of Collichthys lucidus ovum than relatively low；Three be the larvae growth of hatching to postlarva During substantial amounts of death occurs, this may be relevant with its open-mouthed bait series.
The content of the invention
It is an object of the invention to provide a kind of the artificial of the high Collichthys lucidus of rate of fertilization, incubation rate, survival rate of seedling Mating system.
In order to realize the object of the invention, the invention provides a kind of artificial breeding method of Collichthys lucidus, its feature exists In comprising the following steps：
Adult fish that is wild or propagating artificially is fished for as parent：Body length is more than into 10cm, good vigor, not damaged, physique to be good for The Collichthys lucidus male and female individual of strong, gonadal maturation is used as parent；The liquor potassic permanganate that parent is 0.5ppm with concentration Immersion 5 minutes.
Artificial insemination：Using semidry method artificial insemination, the seminal fluid and ovum of Collichthys lucidus are gathered respectively, and seminal fluid is used It is sufficiently mixed after the dilution of Hank ' s liquid with ovum, adds clean seawater, staticly settle 1~2min, takes floating ovum with clean sea It is put into hatchery and hatches after water cleaning；The floating ovum is embryonated egg；
Fry rearing：The fry for hatching is cultivated, fry rearing water temperature is 23~25 DEG C, fry rearing density For 1~20,000 tails/m3；3d starts full pond soil pick-up after hatching, changes water, during soil pick-up delivery port collect residual bait with 80 mesh sieve tulles, it is dead Seedling, excrement；
The density that 4d starts to feed wheel animalcule in the wheel animalcule through splitting kettle algae reinforced cultivating, nursery pond is 5~10ind/mL, even It is continuous to feed 8~15d, feed daily 3~5 times；And since 4d, added in nursery pond split kettle algae solution to maintain in right amount daily Water colour, the concentration for keeping splitting kettle algae in water body is 104~105cell/mL；
The density that 12d starts to feed fairy shrimp in the fairy shrimp through splitting kettle algae reinforced cultivating, nursery pond is 3~5ind/ ML, continuously feeds 3~6d, feeds daily 2~4 times；
It is 1~3ind/mL that 15d, which starts to feed Copepods density in Copepods, nursery pond, and Copepods Feeding time is 10 ~20d, feeds 4~6 times daily；
24d starts to feed mixed feed, feeds daily 2~4 times；Day feeding volume weighs sb. for total fry in nursery pond 3%~6%.
Further, it is described to fish for adult fish that is wild or propagating artificially as in parent's step, the ratio of parent's male and female individual Example is 1:2.
Further, in the artificial insemination step, the method for gathering the seminal fluid of Collichthys lucidus is：Fish body is dried, is squeezed Milter belly is pressed, while the seminal fluid inhalation syringe flowed out at gonopore is continuously sucked into the essence of 4~6 tail milters with syringe After liquid, seminal fluid is squeezed into container, the volume of seminal fluid is recorded, Hank ' the s liquid of 10~20 times of seminal fluid volumes is then added, mixed Close uniform, 4 DEG C of Cord bloods；Optional, the method for gathering the ovum of Collichthys lucidus is：Fish body is dried, raun is gently extruded Belly, makes the ovum of discharge flow into container, after the ovum for continuously extruding 2~3 tail rauns, with regard to carrying out artificial insemination operations.
Further, in the artificial insemination step, there is deep 1 scholar 0.2m clean seawater in the hatchery；Optional, Clean seawater is the seawater by obscure shallow lake, sand filtration processing.When hatchery total depth is 1.4~1.6m, the initial scholar 0.2m of the depth of water 1 Than convenient, convenient operation, and embryonated egg will not be caused to assemble because there is non-water flow region and cause anoxic, can obtain good Effect.
Further, in the artificial insemination step, the hatching refers to embryonated egg in 23~25 DEG C of water temperature, and salinity is 26 Micro- inflation hatching under conditions of~30, hatching density is 1~20,000/m3；It is preferred that, micro- inflation refers to that control water surface spray is straight Footpath is 20cm or so.
Further, in the fry rearing step, through splitting the wheel animalcule and fairy shrimp of kettle algae reinforced cultivating, during reinforced cultivating It is 10 to split kettle algae density7Cell/mL, enhanced time is no less than 12h.
Further, in the fry rearing step, the method for the reinforcing is：Filtering sea is with splitting the emulsification of kettle algae 10min, after pour into and stirred in the container sterilized with potassium permanganate, the wheel animalcule or rich just hatched is collected with 100 mesh sieve tulles Year, worm was thrown in container, and dispensing density is 300~500ind/mL.The standard of filtering sea is GB11607-89 fishery water water Matter standard.Filtering sea and the ratio for splitting kettle algae are 10 during emulsification:1.
Further, in the fry rearing step, the water that changes is that the daily quantity of exchanged water of 3~10d is the pool inner water scale of construction 20%, the daily quantity of exchanged water of 11~15d is the 30% of the pool inner water scale of construction, and daily quantity of exchanged water is the pool inner water scale of construction afterwards 50%.
It is to kill spine that the present invention, which handles just the Collichthys lucidus parent population fished for up or adult fish with liquor potassic permanganate, The harmful microorganism that head baby croaker body surface is carried；When liquor potassic permanganate concentration be more than 0.5ppm when, Collichthys lucidus stress Reaction is stronger, and death occurs when serious, and during less than 0.5ppm, the effect of disinfection can be substantially reduced；Potassium permanganate The speed for releasing oxygen is slow, and soak time reaches that 5min just can effectively kill the harmful microorganisms such as bacterium.
Because Collichthys lucidus milter individual is smaller, seldom, some milters can only extrude a few drop seminal fluid to semen volume, be unfavorable for Artificial insemination, present invention Hank ' s liquid dilution seminal fluid, reaches a certain amount, is convenient for artificial insemination, improve fertilization Rate.
Water temperature control can obtain relatively good effect for 23~25 DEG C during Collichthys lucidus fry rearing.Work as temperature When degree is higher than 25 DEG C, fry abnormal rate can be significantly improved；And when temperature is less than 23 DEG C, the speed of fry growth declines, increase Cultivation cycle and seedling cost.
Fry rearing density is less than 10,000 tails/m3When, the efficiency and benefit of nursery are had influence on, fry rearing density is more than 20,000 Tail/m3When can cause Crowding Stress to fry, cause fry growth speed and survival rate to decline, nursery effect is deteriorated.
In fry rearing step of the present invention, it is the cooperation in order to sop up the unhatched embryonated egg of bottom of pond that 3d, which starts soil pick-up, Carry out row and change water, to maintain water quality totally to clean, ensure fry health, while also playing the role of to stimulate fry initial feeding.
Collichthys lucidus is bred and the optimal salinity of growth is 26~30, and hatchability of fertile eggs highest, educates within this range Seedling effect is best.
The content of the nutrition of wheel animalcule and fairy shrimp especially polyunsaturated fatty acid can not fully meet Collichthys lucidus The need for larva and juvenile grows, thus need reinforcing.The content for splitting kettle algae DHA reaches the 26% of its dry, and chlorella Middle DHA content is relatively low, and only 5% or so；Under similarity condition, wheel animalcule or artemia ratio chlorella reinforcing institute are strengthened with kettle algae is split The time needed is short, and reinforcing effect is more preferable.Because wheel animalcule individual is small, quantity is big, density is high, it is necessary to concentration to split kettle algae solution strong Change, satisfied fortification effect can be reached.Fairy shrimp can with concentration, also can use that not concentrated to split kettle algae solution strong Change.
In fry rearing step, the kettle concentration of algae that splits of reinforcing wheel animalcule is 107Cell/mL, enhanced time is no less than 12h； The kettle algae solution concentration of splitting of reinforcing fairy shrimp is 0.5g/L.Because the concentration for splitting kettle algae is less than 107During cell/mL, when wheel animalcule is strengthened Between it is longer, reinforcing effect can also be deteriorated；Enhanced time is less than 12h, can reduce unsaturated in wheel animalcule reinforcing effect, i.e. wheel animalcule body The accumulation of aliphatic acid can not reach ideal value, and then influence fry growth development.
In Collichthys lucidus parent, the body weight of general raun is 1~3 times of milter, and the semen volume of milter is less, if Milter (such as raun very little:Milter=1:1), it may be difficult to ensure the rate of fertilization of ovum.The sex ration of parent of the present invention is 1:2, It ensure that ovum is fully fertilized, improve rate of fertilization.
The mating period of Collichthys lucidus, in 4~June, fishes for Collichthys lucidus parent May for Best Times, fished for During as far as possible band water operation, it is ensured that Collichthys lucidus survive and not damaged.
Present invention delivery port in soil pick-up is collected with 80 mesh sieve tulles, checks residual bait, dead seedling, excrement, so as to understanding in time Fry ingests situation and health status, is used as regulating water quality, the foundation of bait feeding amount.
The present invention improves the rate of fertilization, incubation rate and survival rate of fish fry of Collichthys lucidus, is that Collichthys lucidus is manually supported Grow and enough seeds are provided.
The present invention compared with prior art, with advantage following prominent：
1) by controlling extraction and the diluted concentration of Collichthys lucidus seminal fluid, the ratio of smart ovum mixing in technical solution of the present invention Example and time of insemination, significantly improve rate of fertilization, incubation rate and survival rate of seedling；
2) use and split kettle algae as wheel animalcule hardening agent, it is better than with chlorella reinforcing effect, greatly improve survival rate of seedling.
Embodiment
Embodiments of the invention are described below in detail, the example of the embodiment is intended to be used to explain the present invention, and can not It is interpreted as limitation of the present invention.In the examples where no specific technique or condition is specified, according to described by document in the art Technology or condition or carried out according to product description.Agents useful for same or the unreceipted production firm person of instrument, are to lead to Cross the conventional products of acquisition purchased in market.
Embodiment 1：Artificial breeding is carried out with the adult fish of wild Collichthys lucidus
Wild adult fish is fished for as parent：In late April, in white rock-block field domain under Fujian Province's Ningde City, in local fishermen During fish operation, good vigor, not damaged, physically fit, gonadal maturation the tail of Collichthys lucidus 864 are collected from otter-trawl, Be transported to nursery, perforated ship cabin covered with sunshade net, to prevent strong light stimulus cause Collichthys lucidus excessively stress and it is dead.Will The liquor potassic permanganate that the Collichthys lucidus transported back is 0.5ppm with concentration soaks 5 minutes, is then placed in nursery pond, micro- to fill Gas.
Good vigor, not damaged, physically fit, gonadal maturation male and female are selected in the Collichthys lucidus fished for up Individual is used as parent, raun criteria for selection：Belly is full, expansion, soft flexible, gonopore pale red, micro- prominent；Milter selects Standard：Belly softness is flexible, gently presses postabdomen, there is seminal fluid outflow, and it is to dissipate to meet water, and sex ration is 1:2.
Artificial insemination：Using semidry method artificial insemination, milter is taken out, with paper handkerchief by the water wiped clean of fish body table, gently The male parent population belly of extruding, at the same with 1mL syringe by the seminal fluid inhalation syringe discharged at gonopore (due to Collichthys lucidus Semen volume it is considerably less, one can hardly be extruded and drip off whole seminal fluid), if seminal fluid is undesirable (amount less and transparent color), Milter is then selected again, squeezes into seminal fluid in disposal plastic cup after continuously sucking 4~6 tail milters, records the volume of seminal fluid, so After add Hank ' s liquid, the volume ratio of Collichthys lucidus sperm and Hank ' s liquid is 1:10~20, gently rocking plastic cup makes The two is well mixed, is put into preprepared dry ice and cools in the closed plastic bubble chamber of 4 DEG C of low temperature；Raun is taken out, is used The water wiped clean of fish body table is gently extruded dam's fish belly portion, the ovum of discharge is dropped down in disposal plastic cup by paper handkerchief, due to Ovum amount is few, after the raun of 2~3 tails of general continuous extrusion, adds the seminal fluid by dilution, the volume ratio of smart ovum is 1:10~ 20, plastic cup is gently rocked, smart ovum is had the chance fully contacted, clean seawater is added after fully mixing, 1~2min is precipitated Afterwards, take floating ovum to be cleaned once with clean seawater, be finally putting into micro- inflation hatching in cement pit.Nursery pond ambient light sunshade Net regulation is kept quite to 200~500Lux；
The clean seawater is that, by obscure shallow lake, the seawater of sand filtration in nursery, water temperature is 23~24 DEG C, and salinity is 28；
The formula of Hank ' the s liquid is：KCl 0.4g/L,NaCl 8.01g/L,CaCl2 0.14g/L,NaHCO3 0.35g/L,KH2PO4 0.06g/L,Glucose 0.34g/L,pH 8.0。
Fry rearing：Collichthys lucidus embryonated egg is hatched since 20h, all hatches to 30h, fry rearing water temperature Control is between 23~25 DEG C, and fry rearing density is 1~20,000 tails/m3；3d starts full pond soil pick-up after hatching, changes water, soil pick-up When delivery port collected with 80 mesh sieve tulles, check residual bait, dead seedling, excrement, ingested situation and health to understand fry in time Situation, is used as regulating water quality, the foundation of bait feeding amount；It is that the daily quantity of exchanged water of 3~10d is the pool inner water scale of construction to change water The daily quantity of exchanged water of 20%, 11~15d is the 30% of the pool inner water scale of construction, and daily quantity of exchanged water is the 50% of the pool inner water scale of construction afterwards； It is 5~10ind/mL that 4d, which starts to feed wheel animalcule density in the concentrated wheel animalcule for splitting the reinforcing of kettle algae, nursery pond, after hatching, daily Feed 4~6 times, in nursery pond since being fed wheel animalcule, add split kettle algae solution to maintain water colour daily, keep water stabilization； It is 3~5ind/mL that 12d, which starts to feed fairy shrimp density in the fairy shrimp through splitting the reinforcing of kettle algae solution, nursery pond, and 2 are fed daily ~4 times；It is 1~3ind/mL that 15d, which starts to feed Copepods density in Copepods, nursery pond, is fed daily 4~6 times；24d Start to feed mixed feed, feed daily 4~6 times, day feeding volume is total fry weighs sb. in nursery pond 3%~6%.
During to 60d, fry average length 2.2cm.Smart ovum is gathered with 693 tail Collichthys lucidus parent populations altogether, is carried out artificial Insemination, fertilization rate reached to 74.5%, incubation rate reaches that 86.6%, 2.2cm survival rate of fish fry reaches 58.6%, therefore the present invention Technical scheme improve the rate of fertilization, incubation rate and survival rate of fish fry of Collichthys lucidus, be the full artificial breeding of Collichthys lucidus Lay the foundation, be that Collichthys lucidus propagates the enough seeds of offer artificially.
Although embodiments of the invention have been shown and described above, it is to be understood that above-described embodiment is example Property, it is impossible to limitation of the present invention is interpreted as, one of ordinary skill in the art is not departing from the principle and objective of the present invention In the case of above-described embodiment can be changed within the scope of the invention, change, replace and modification.

Claims (8)

1. a kind of artificial breeding method of Collichthys lucidus, it is characterised in that comprise the following steps：
Adult fish is fished for as parent：Body length is more than 10cm, good vigor, not damaged, physically fit, gonadal maturation spine head Baby croaker male and female individual is used as parent；
Artificial insemination：Using semidry method artificial insemination, the seminal fluid and ovum of Collichthys lucidus, seminal fluid Hank ' s liquid are gathered respectively It is sufficiently mixed after dilution with ovum, adds clean seawater, staticly settle 1~2min, is taken after floating ovum cleans with clean seawater It is put into hatchery and hatches；The floating ovum is embryonated egg；
Fry rearing：The fry for hatching is cultivated, fry rearing water temperature is 23~25 DEG C, fry rearing density is 1 ~2 ten thousand tails/m3；3d starts full pond soil pick-up after hatching, changes water, during soil pick-up delivery port with 80 mesh sieve tulles collect residual bait, dead seedling, Excrement；
4d starts to feed the wheel animalcule through splitting kettle algae reinforced cultivating, and the density for making wheel animalcule in nursery pond is 5~10ind/mL, continuously 8~15d is fed, is fed daily 3~5 times；And since 4d, added in nursery pond split kettle algae solution to maintain water in right amount daily Color, the concentration for keeping splitting kettle algae in water body is 104~105cell/mL；
12d starts to feed the fairy shrimp through splitting kettle algae solution reinforced cultivating, and the density for making fairy shrimp in nursery pond is 3~5ind/ ML, continuously feeds 3~6d, feeds daily 2~4 times；
It is 1~3ind/mL that 15d, which starts to feed in Copepods, nursery pond Copepods density, Copepods Feeding time is 10~ 20d, feeds 4~6 times daily；
24d starts to feed mixed feed, feeds daily 2~4 times；Day feeding volume is total fry weighs sb. in nursery pond 3% ~6%.
2. the artificial breeding method of Collichthys lucidus according to claim 1, it is characterised in that described to fish for adult fish as parent In this step, the ratio of parent's male and female individual is 1:2.
3. the artificial breeding method of Collichthys lucidus according to claim 1, it is characterised in that the artificial insemination step In, the method for gathering the seminal fluid of Collichthys lucidus is：Fish body is dried, milter belly is extruded, while with syringe by gonopore The seminal fluid inhalation syringe of discharge, continuously sucks after the seminal fluid of 4~6 tail milters, seminal fluid is squeezed into container, records the body of seminal fluid Product, then adds Hank ' the s liquid of 10~20 times of seminal fluid volumes, is well mixed, 4 DEG C of Cord bloods；Optional, gather spine head The method of the ovum of baby croaker is：Fish body is dried, raun belly is extruded, the ovum of discharge is dropped down in container, 2~3 are continuously extruded The raun of tail.
4. the artificial breeding method of Collichthys lucidus according to claim 1, it is characterised in that the artificial insemination step In, there is deep 1 scholar 0.2m clean seawater in the hatchery；Optional, clean seawater is the sea by obscure shallow lake, sand filtration processing Water.
5. the artificial breeding method of Collichthys lucidus according to claim 1, it is characterised in that the artificial insemination step In, the hatching refers to embryonated egg in 23~25 DEG C of water temperature, and salinity is micro- inflation hatching under conditions of 26~30, and hatching density is 1~20,000/m3；It is preferred that, micro- inflation refers to a diameter of 20cm of control water surface spray.
6. the artificial breeding method of Collichthys lucidus according to claim 1, it is characterised in that the fry rearing step In, through splitting the wheel animalcule and fairy shrimp of kettle algae reinforced cultivating, it is 10 that kettle algae density is split during reinforced cultivating7Cell/mL, enhanced time is not Less than 12h.
7. the artificial breeding method of Collichthys lucidus according to claim 1, it is characterised in that the fry rearing step In, the method for the reinforcing is：Filtering sea and split kettle algae emulsification 10min, after pour into and stirred in the container sterilized with potassium permanganate Mix uniform, collect the wheel animalcule just hatched with 100 mesh sieve tulles or fairy shrimp is thrown in container, dispensing density is 300~500ind/ mL。
8. the artificial breeding method of Collichthys lucidus according to claim 1, it is characterised in that the fry rearing step In, the water that changes is that the daily quantity of exchanged water of 3~10d is the 20% of the pool inner water scale of construction, and the daily quantity of exchanged water of 11~15d is pool inner water The 30% of the scale of construction, daily quantity of exchanged water is the 50% of the pool inner water scale of construction afterwards.
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CN110622890A (en) * 2019-10-28 2019-12-31 宁德市富发水产有限公司 Method for rapid artificial fertilization of wild stone head fish
CN111264423A (en) * 2020-03-27 2020-06-12 浙江省海洋水产养殖研究所 Seedling raising method for acanthocephalus spinosus
CN113383732A (en) * 2021-07-27 2021-09-14 中国水产科学研究院东海水产研究所 Artificial insemination and incubation method for Oxytropis spinosa juvenile fish
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CN114009377A (en) * 2021-11-15 2022-02-08 象山港湾水产苗种有限公司 Industrial breeding method and full-artificial breeding method for acanthocephalus molitrix

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CN110622890A (en) * 2019-10-28 2019-12-31 宁德市富发水产有限公司 Method for rapid artificial fertilization of wild stone head fish
CN111264423A (en) * 2020-03-27 2020-06-12 浙江省海洋水产养殖研究所 Seedling raising method for acanthocephalus spinosus
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CN113383732A (en) * 2021-07-27 2021-09-14 中国水产科学研究院东海水产研究所 Artificial insemination and incubation method for Oxytropis spinosa juvenile fish
CN114009377A (en) * 2021-11-15 2022-02-08 象山港湾水产苗种有限公司 Industrial breeding method and full-artificial breeding method for acanthocephalus molitrix

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