CN107114283B - A kind of artificial breeding method of Collichthys lucidus - Google Patents
A kind of artificial breeding method of Collichthys lucidus Download PDFInfo
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- 241000960974 Collichthys lucidus Species 0.000 title claims abstract description 57
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- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 25
- 210000004681 ovum Anatomy 0.000 claims abstract description 25
- 230000000384 rearing effect Effects 0.000 claims abstract description 21
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- 230000012447 hatching Effects 0.000 claims abstract description 17
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- 238000010276 construction Methods 0.000 claims description 9
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- 210000001015 abdomen Anatomy 0.000 claims description 8
- 235000013601 eggs Nutrition 0.000 claims description 8
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- 230000008859 change Effects 0.000 claims description 5
- 238000007667 floating Methods 0.000 claims description 5
- 230000003014 reinforcing effect Effects 0.000 claims description 5
- 241001417494 Sciaenidae Species 0.000 claims description 4
- 238000001914 filtration Methods 0.000 claims description 4
- 230000002710 gonadal effect Effects 0.000 claims description 4
- 230000035800 maturation Effects 0.000 claims description 4
- 239000012286 potassium permanganate Substances 0.000 claims description 3
- 238000012545 processing Methods 0.000 claims description 3
- 238000009287 sand filtration Methods 0.000 claims description 3
- 238000005138 cryopreservation Methods 0.000 claims description 2
- 238000010790 dilution Methods 0.000 claims description 2
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- 239000007921 spray Substances 0.000 claims description 2
- 238000003756 stirring Methods 0.000 claims description 2
- 230000001488 breeding effect Effects 0.000 abstract description 10
- 230000004720 fertilization Effects 0.000 abstract description 9
- 230000004083 survival effect Effects 0.000 abstract description 9
- 238000002156 mixing Methods 0.000 abstract description 3
- 238000000605 extraction Methods 0.000 abstract description 2
- 230000000694 effects Effects 0.000 description 11
- 238000005728 strengthening Methods 0.000 description 6
- 239000000047 product Substances 0.000 description 4
- 210000000582 semen Anatomy 0.000 description 4
- 241000195649 Chlorella <Chlorellales> Species 0.000 description 3
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
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- 241000894006 Bacteria Species 0.000 description 1
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- 241000960973 Collichthys Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 239000007836 KH2PO4 Substances 0.000 description 1
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- 244000144987 brood Species 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
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- 210000004027 cell Anatomy 0.000 description 1
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- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
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- JEGUKCSWCFPDGT-UHFFFAOYSA-N h2o hydrate Chemical compound O.O JEGUKCSWCFPDGT-UHFFFAOYSA-N 0.000 description 1
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- 235000013372 meat Nutrition 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
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- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 235000020777 polyunsaturated fatty acids Nutrition 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Insects & Arthropods (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses a kind of artificial breeding methods of Collichthys lucidus, belong to fish reproduction field, and step includes: fishing adult fish as parent, artificial insemination and fry rearing.The present invention passes through the extraction and diluted concentration of Collichthys lucidus sperm, the ratio and time of insemination of smart ovum mixing, the rate of fertilization, hatching rate and survival rate of fish fry of Collichthys lucidus are improved, is laid the foundation for the full artificial breeding of Collichthys lucidus, provides enough seeds for Collichthys lucidus artificial breeding.
Description
Technical field
The invention belongs to fish reproduction fields, and in particular to a kind of artificial breeding method of Collichthys lucidus.
Background technique
Collichthys lucidus (Collichthys lucidus) belongs to Perciformes, and Sciaenidae, Collichthy is China coastal seas
The common small-sized economic fish of dwelling property of bottom, is mainly distributed on the East Sea and the Huanghai Sea, to the east of marine products amounts it is maximum.Collichthys lucidus is short distance
Shallow sea fish from migration, happiness are inhabited in river mouth salt-fresh water intersection, and the speed of growth is fast, and thermophilic, proper salinity level are wide, and meat is thin
One of high-grade aquatic products that are tender and delicious, being fond of for coastal resident.In recent years, due to the originals such as overfishing, offshore pollution be serious
Cause causes the natural resources amount of Collichthys lucidus to be greatly decreased, and the demand of artificial propagation techniques and cultural technique is come into being,
But a whole set of current still not formed effective Collichthys lucidus artificial breeding technology, the quantization that cannot achieve artificial culture fry are raw
It produces, so that it is propagated industry artificially can not also develop.The main bottleneck of Collichthys lucidus artificial breeding has, first is that of Collichthys lucidus
Body is smaller, and female fish brood amount is not high, and male semen volume is less, and male and female fish gonad development is asynchronous so that it is a large amount of obtain by
Smart ovum is extremely difficult；Second is that the fertility rate and hatchability of Collichthys lucidus ovum is relatively low；Third is that the larvae growth of hatching is to postlarva
During will appear a large amount of death, this may be related with its open-mouthed bait series.
Summary of the invention
The purpose of the present invention is to provide a kind of the artificial of the high Collichthys lucidus of rate of fertilization, hatching rate, survival rate of seedling
Mating system.
In order to achieve the object of the present invention, the present invention provides a kind of artificial breeding method of Collichthys lucidus, feature exists
In, comprising the following steps:
Wild or artificial breeding adult fish is caught as parent: by body it is long greater than 10cm, vigor is good, not damaged, physique is good for
Strong, gonadal maturation Collichthys lucidus male and female individual is as parent；The liquor potassic permanganate that parent is 0.5ppm with concentration
It impregnates 5 minutes.
Artificial insemination: using semidry method artificial insemination, acquires the sperm and ovum of Collichthys lucidus respectively, and sperm is used
It is sufficiently mixed after the dilution of Hank ' s liquid with ovum, adds clean seawater, staticly settle 1~2min, take floating ovum with clean sea
It is put into hatchery and hatches after water cleaning；The floating ovum is fertilized eggs；
Fry rearing: the fry come will be hatched and cultivated, fry rearing water temperature is 23~25 DEG C, fry rearing density
For 1~20,000 tails/m3；3d starts full pond soil pick-up after hatching, changes water, and water outlet collects residual bait, dead with 80 mesh sieve tulles when soil pick-up
Seedling, excrement；
4d starts to feed the wheel animalcule through splitting pot algae reinforced cultivating, and the density of wheel animalcule is 5~10ind/mL in nursery pond, even
It is continuous to feed 8~15d, it feeds daily 3~5 times；And since 4d, it is added in nursery pond splits pot algae solution in right amount to maintain daily
Water colour, keeping the concentration that pot algae is split in water body is 104~105cell/mL；
12d starts to feed the fairy shrimp through splitting pot algae reinforced cultivating, and the density of fairy shrimp is 3~5ind/ in nursery pond
ML, continuously feeds 3~6d, feeds daily 2~4 times；
15d starts to feed Copepods, and Copepods density is 1~3ind/mL in nursery pond, and Copepods Feeding time is 10
~20d is fed 4~6 times daily；
24d starts to feed mixed feed, feeds daily 2~4 times；Day feeding volume is that total fry weighs sb. in nursery pond
3%~6%.
Further, the fishing is wild or the adult fish of artificial breeding is as in parent's step, the ratio of parent's male and female individual
Example is 1:2.
Further, in the artificial insemination step, the method that acquires the sperm of Collichthys lucidus are as follows: dry fish body, squeeze
Milter abdomen, while the sperm inhalation syringe that will be flowed out at gonopore with syringe are pressed, the essence of 4~6 tail milters is continuously sucked
After liquid, sperm is squeezed into container, records the volume of sperm, Hank ' the s liquid of 10~20 times of sperm volumes is then added, is mixed
It closes uniformly, 4 DEG C of cryo-conservations；Optional, the method for acquiring the ovum of Collichthys lucidus are as follows: dry fish body, gently squeeze raun
Abdomen flows into the ovum of discharge in container, after the ovum for continuously squeezing out 2~3 tail rauns, with regard to carrying out artificial insemination operations.
Further, in the artificial insemination step, there is the clean seawater of deep 1 scholar 0.2m in the hatchery；Optional,
Clean seawater is the seawater by obscure shallow lake, sand filtration processing.When hatchery total depth is 1.4~1.6m, initial 1 scholar 0.2m of the depth of water
Than convenient, facilitate operation, and not will lead to fertilized eggs and assemble due to there are non-water flow region and lead to anoxic, can obtain good
Effect.
Further, in the artificial insemination step, the hatching refer to fertilized eggs at 23~25 DEG C of water temperature, salinity 26
Micro- inflation hatching, hatching density are 1~20,000/m under conditions of~303；Preferably, micro- inflation refers to that control water surface spray is straight
Diameter is 20cm or so.
Further, in the fry rearing step, the wheel animalcule and fairy shrimp of pot algae reinforced cultivating are split, when reinforced cultivating
Splitting pot algae density is 107Cell/mL, enhanced time are no less than 12h.
Further, in the fry rearing step, the method for the reinforcing are as follows: filtering sea is emulsified with pot algae is split
10min, after pour into the container sterilized with potassium permanganate and stir evenly, collect the wheel animalcule that has just hatched or rich with 100 mesh sieve tulles
Year, worm was thrown in container, and throw-in density is 300~500ind/mL.The standard of filtering sea is GB11607-89 fishery water water
Matter standard.Filtering sea and the ratio for splitting pot algae are 10:1 when emulsification.
Further, in the fry rearing step, described to change water be the daily quantity of exchanged water of 3~10d is the pool inner water scale of construction
20%, the daily quantity of exchanged water of 11~15d is the 30% of the pool inner water scale of construction, and daily quantity of exchanged water is the pool inner water scale of construction later
50%.
The present invention just catches the Collichthys lucidus parent population to come up with liquor potassic permanganate processing or adult fish is to kill spine
The harmful microorganism that head baby croaker body surface carries；When liquor potassic permanganate concentration be greater than 0.5ppm when, Collichthys lucidus stress
React stronger, when will appear death when serious, and being less than 0.5ppm, the effect of disinfection and sterilization be will be greatly reduced；Potassium permanganate
The speed for releasing oxygen is slow, and soaking time, which reaches 5min, just can effectively kill the harmful microorganisms such as bacterium.
Since Collichthys lucidus milter individual is smaller, semen volume is seldom, some milters can only squeeze out a few drop sperm, is unfavorable for
Artificial insemination, present invention Hank ' s liquid dilute sperm, reach a certain amount, are convenient for artificial insemination, improve fertilization
Rate.
Water temperature control can obtain relatively good effect for 23~25 DEG C during Collichthys lucidus fry rearing.Work as temperature
When degree is higher than 25 DEG C, fry abnormal rate can be significantly improved；And when temperature is lower than 23 DEG C, the speed decline of fry growth increases
Cultivation cycle and seedling cost.
Fry rearing density is less than 10,000 tails/m3When, the efficiency and benefit of nursery are influenced, fry rearing density is greater than 20,000
Tail/m3When can cause Crowding Stress to fry, cause fry growth speed and survival rate to decline, nursery effect is deteriorated.
In fry rearing step of the present invention, it is the unhatched fertilized eggs in order to sop up bottom of pond that 3d, which starts soil pick-up, cooperation
The row of progress changes water, to maintain water quality completely to clean, ensures fry health, while the also effect of irritating fry initial feeding.
The optimal salinity of Collichthys lucidus breeding and growth is 26~30, and hatchability of fertile eggs highest, educates within this range
Seedling effect is best.
The content of the nutrition of wheel animalcule and fairy shrimp especially polyunsaturated fatty acid can not fully meet Collichthys lucidus
The needs of larva and juvenile growth and development, thus need to strengthen.The content for splitting pot algae DHA reaches the 26% of its dry matter, and chlorella
The content of middle DHA is lower, and only 5% or so；Under similarity condition, strengthen wheel animalcule or artemia ratio chlorella reinforcing institute with pot algae is split
The time needed wants short, and strengthening effect is more preferable.Since wheel animalcule individual is small, quantity is big, density is high, need with concentration to split pot algae solution strong
Change, satisfied fortification effect can be reached.Fairy shrimp can with concentration, also can be used that not concentrated to split pot algae solution strong
Change.
In fry rearing step, the pot concentration of algae that splits for strengthening wheel animalcule is 107Cell/mL, enhanced time are no less than 12h；
The pot algae solution concentration of splitting for strengthening fairy shrimp is 0.5g/L.Because splitting the concentration of pot algae lower than 107When cell/mL, when wheel animalcule is strengthened
Between want longer, strengthening effect can also be deteriorated；Enhanced time is less than 12h, can reduce wheel animalcule strengthening effect, i.e., unsaturated in wheel animalcule body
The accumulation of fatty acid cannot reach ideal value, and then influence fry growth development.
In Collichthys lucidus parent, the weight of general raun is 1~3 times of milter, and the semen volume of milter is less, if
Milter is very little (such as raun: milter=1:1), it may be difficult to guarantee the rate of fertilization of ovum.The sex ration of parent of the present invention is 1:2,
It ensure that ovum is sufficiently fertilized, improve rate of fertilization.
The mating period of Collichthys lucidus, catching Collichthys lucidus parent May was Best Times, fishing in 4~June
In the process as far as possible band water operate, guarantee Collichthys lucidus survival and it is not damaged.
Present invention water outlet in soil pick-up is collected with 80 mesh sieve tulles, residual bait, dead seedling, excrement is checked, to understand in time
Fry ingests situation and health status, as regulating water quality, the foundation of bait feeding amount.
The present invention improves the rate of fertilization, hatching rate and survival rate of fish fry of Collichthys lucidus, manually supports for Collichthys lucidus
It grows and enough seeds is provided.
Compared with prior art, the present invention having the following prominent advantages:
1) pass through the extraction and diluted concentration of control Collichthys lucidus sperm, the ratio of smart ovum mixing in technical solution of the present invention
Example and time of insemination, significantly improve rate of fertilization, hatching rate and survival rate of seedling；
2) it uses and splits pot algae as wheel animalcule hardening agent, it is better than with chlorella strengthening effect, greatly improve survival rate of seedling.
Specific embodiment
The embodiment of the present invention is described below in detail, the examples of the embodiments are intended to be used to explain the present invention, and cannot
It is interpreted as limitation of the present invention.In the examples where no specific technique or condition is specified, described according to the literature in the art
Technology or conditions or carried out according to product description.Reagents or instruments used without specified manufacturer is that can lead to
Cross the conventional products of commercially available acquisition.
Embodiment 1: artificial breeding is carried out with the adult fish of wild Collichthys lucidus
Wild adult fish is caught as parent: in late April, the white rock-block field domain under Fujian Province's Ningde City, in local fishermen
When fish operation, collect that vigor is good, not damaged, physically fit, gonadal maturation 864 tail of Collichthys lucidus from otter-trawl,
Be transported to nursery, cover perforated ship cabin with sunshade net, to prevent strong light stimulus cause Collichthys lucidus excessively stress and it is dead.It will
The Collichthys lucidus transported back is impregnated 5 minutes with the liquor potassic permanganate that concentration is 0.5ppm, is then placed in nursery pond, micro- to fill
Gas.
Select that vigor is good, not damaged, physically fit, gonadal maturation male and female in the Collichthys lucidus that comes up of fishing
Individual is used as parent, and raun criteria for selection: abdomen is full, expansion, soft flexible, gonopore pale red, micro- prominent；Milter selects
Standard: abdomen softness is flexible, gently presses postabdomen, there is sperm outflow, meets water and dissipates, sex ration 1:2.
Artificial insemination: using semidry method artificial insemination, takes out milter, with paper handkerchief by the water wiped clean of fish body table, gently
Squeeze male parent population abdomen, at the same with the syringe of 1mL by the sperm inhalation syringe being discharged at gonopore (due to Collichthys lucidus
Semen volume it is considerably less, the complete sperm of a drop can hardly be squeezed out), if sperm is undesirable (amount less and transparent color),
Milter is then selected again, and sperm is squeezed into disposal plastic cup after continuously sucking 4~6 tail milters, records the volume of sperm, so
After be added Hank ' s liquid, the volume ratio of Collichthys lucidus sperm and Hank ' s liquid is 1:10~20, and shaking gently plastic cup makes
The two is uniformly mixed, and is put into preprepared dry ice and is cooled in the closed plastic bubble chamber of 4 DEG C of low temperature；Raun is taken out, is used
The water wiped clean of fish body table is gently squeezed female parent's fish belly portion by paper handkerchief, drops down the ovum of discharge in disposal plastic cup, due to
Ovum amount is few, after the general continuous raun for squeezing out 2~3 tails, is added and passes through diluted sperm, the volume ratio of smart ovum be 1:10~
20, plastic cup is shaked gently, smart ovum is made to have the chance come into full contact with, clean seawater is added after mixing well, precipitates 1~2min
Afterwards, it takes floating ovum to be cleaned once with clean seawater, is finally putting into micro- inflation hatching in cement pit.Nursery pond ambient light sunshade
Net is adjusted to 200~500Lux, and keeps quite；
The clean seawater is by obscure shallow lake, the seawater of sand filtration in nursery, and water temperature is 23~24 DEG C, salinity 28；
The formula of Hank ' the s liquid are as follows: KCl 0.4g/L, NaCl 8.01g/L, CaCl2 0.14g/L, NaHCO3
0.35g/L,KH2PO4 0.06g/L,Glucose 0.34g/L,pH 8.0。
Fry rearing: Collichthys lucidus fertilized eggs are hatched since 20h, are come until 30h is all hatched, fry rearing water temperature
Between 23~25 DEG C, fry rearing density is 1~20,000 tails/m for control3；3d starts full pond soil pick-up after hatching, changes water, soil pick-up
When water outlet collected with 80 mesh sieve tulles, check residual bait, dead seedling, excrement, ingest situation and health to understand fry in time
Situation, as regulating water quality, the foundation of bait feeding amount；It is the pool inner water scale of construction that change water, which be the daily quantity of exchanged water of 3~10d,
The daily quantity of exchanged water of 20%, 11~15d is the 30% of the pool inner water scale of construction, and daily quantity of exchanged water is the 50% of the pool inner water scale of construction later；
4d starts to feed the concentrated wheel animalcule for splitting the reinforcing of pot algae after hatching, and wheel animalcule density is 5~10ind/mL in nursery pond, daily
It feeds 4~6 times, in nursery pond since being fed wheel animalcule, is added splits pot algae solution to maintain water colour daily, keep water stabilization；
12d starts to feed the fairy shrimp through splitting the reinforcing of pot algae solution, and fairy shrimp density is 3~5ind/mL in nursery pond, feeds 2 daily
~4 times；15d starts to feed Copepods, and Copepods density is 1~3ind/mL in nursery pond, feeds daily 4~6 times；24d
Start to feed mixed feed, feed daily 4~6 times, day feeding volume is total fry weighs sb. in nursery pond 3%~6%.
When to 60d, fry average length 2.2cm.Smart ovum is acquired with 693 tail Collichthys lucidus parent populations in total, is carried out artificial
Insemination, fertilization rate reached to 74.5%, the survival rate of fish fry that hatching rate reaches 86.6%, 2.2cm reach 58.6%, therefore the present invention
Technical solution improve the rate of fertilization, hatching rate and survival rate of fish fry of Collichthys lucidus, be the full artificial breeding of Collichthys lucidus
It lays the foundation, provides enough seeds for Collichthys lucidus artificial breeding.
Although the embodiments of the present invention has been shown and described above, it is to be understood that above-described embodiment is example
Property, it is not considered as limiting the invention, those skilled in the art are not departing from the principle of the present invention and objective
In the case where can make changes, modifications, alterations, and variations to the above described embodiments within the scope of the invention.
Claims (7)
1. a kind of artificial breeding method of Collichthys lucidus, which comprises the following steps:
Adult fish is caught as parent: by body it is long greater than 10cm, vigor is good, not damaged, physically fit, gonadal maturation spine head
Baby croaker male and female individual is as parent；
Artificial insemination: using semidry method artificial insemination, acquires the sperm and ovum of Collichthys lucidus, sperm Hank ' s liquid respectively
It is sufficiently mixed after dilution with ovum, the volume ratio of Collichthys lucidus sperm and Hank ' s liquid is 1:10~20, is added clean
Seawater staticly settles 1~2min, is put into hatchery and hatches after taking floating ovum to be cleaned with clean seawater, has in the hatchery
The clean seawater of deep 1 scholar 0.2m, clean seawater are the seawater by obscure shallow lake, sand filtration processing；The hatching refers to that fertilized eggs exist
23~25 DEG C of water temperature, micro- inflation is hatched under conditions of salinity is 26~30, and hatching density is 1~20,000/m3；The floating ovum
As fertilized eggs；
Fry rearing: will hatch the fry come and cultivate, and fry rearing water temperature is 23~25 DEG C, and fry rearing density is 1
~2 ten thousand tails/m3；3d starts full pond soil pick-up after hatching, changes water, when soil pick-up water outlet with 80 mesh sieve tulles collect residual bait, dead seedling,
Excrement；
4d starts to feed the wheel animalcule through splitting pot algae reinforced cultivating, makes 5~10ind/mL of density of wheel animalcule in nursery pond, continuously
8~15d is fed, is fed daily 3~5 times；And since 4d, it is added in nursery pond splits pot algae solution in right amount to maintain water daily
Color, keeping the concentration that pot algae is split in water body is 104~105cell/mL；
12d starts to feed the fairy shrimp through splitting pot algae solution reinforced cultivating, makes 3~5ind/ of density of fairy shrimp in nursery pond
ML, continuously feeds 3~6d, feeds daily 2~4 times；
15d starts to feed Copepods, in nursery pond Copepods density be 1~3ind/mL, Copepods Feeding time be 10~
20d is fed 4~6 times daily；
24d starts to feed mixed feed, feeds daily 2~4 times；Day feeding volume is total fry weighs sb. in nursery pond 3%
~6%.
2. the artificial breeding method of Collichthys lucidus according to claim 1, which is characterized in that the fishing adult fish is as parent
In this step, the ratio of parent's male and female individual is 1:2.
3. the artificial breeding method of Collichthys lucidus according to claim 1, which is characterized in that the artificial insemination step
In, the method that acquires the sperm of Collichthys lucidus are as follows: dry fish body, squeeze milter abdomen, while will be at gonopore with syringe
Sperm is squeezed into container after continuously sucking the sperm of 4~6 tail milters, records the body of sperm by the sperm inhalation syringe of discharge
Then Hank ' the s liquid of 10~20 times of sperm volumes is added in product, be uniformly mixed, 4 DEG C of cryo-conservations；Optional, acquire spine head
The method of the ovum of baby croaker are as follows: dry fish body, squeeze raun abdomen, drop down the ovum of discharge in container, continuously squeeze out 2~3
The raun of tail.
4. the artificial breeding method of Collichthys lucidus according to claim 1, which is characterized in that the artificial insemination step
In, micro- inflation refers to that control water surface spray diameter is 20cm.
5. the artificial breeding method of Collichthys lucidus according to claim 1, which is characterized in that the fry rearing step
In, split the wheel animalcule and fairy shrimp of pot algae reinforced cultivating, it is 10 that when reinforced cultivating, which splits pot algae density,7Cell/mL, enhanced time is not
Less than 12h.
6. the artificial breeding method of Collichthys lucidus according to claim 1, which is characterized in that the fry rearing step
In, the method for the reinforcing are as follows: filtering sea and split pot algae emulsification 10min, after pour into potassium permanganate sterilize container in stir
It mixes uniformly, collects the wheel animalcule just hatched with 100 mesh sieve tulles or fairy shrimp is thrown in container, throw-in density is 300~500ind/
mL。
7. the artificial breeding method of Collichthys lucidus according to claim 1, which is characterized in that the fry rearing step
In, described to change water be the daily quantity of exchanged water of 3~10d is the 20% of the pool inner water scale of construction, and the daily quantity of exchanged water of 11~15d is pool inner water
The 30% of the scale of construction, daily quantity of exchanged water is the 50% of the pool inner water scale of construction later.
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