CN111316940A - Artificial breeding method of takifugu obscurus - Google Patents
Artificial breeding method of takifugu obscurus Download PDFInfo
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- CN111316940A CN111316940A CN202010336498.4A CN202010336498A CN111316940A CN 111316940 A CN111316940 A CN 111316940A CN 202010336498 A CN202010336498 A CN 202010336498A CN 111316940 A CN111316940 A CN 111316940A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/17—Hatching, e.g. incubators
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention provides an artificial breeding method of takifugu obscurus, which comprises the following steps: parent fish source and screening, parent fish intensive cultivation, parent fish spawning induction and artificial incubation; the artificial breeding method of the invention strengthens the nutrition of the parent of the takifugu obscurus, coordinates the technological conditions in temperature, salinity, dissolved oxygen, pH value, oxygenation power, ammonia nitrogen and nitroso nitrogen, ensures that the parent can mature gonad under the condition of artificial breeding, and obtains a large amount of fertilized eggs for artificial incubation through artificial induced spawning and incubation. The parent fish has high spawning rate, high fertilized egg rate and hatching rate and high newly hatched fry activity, and can meet the requirement of large scale cultivation of takifugu obscurus fry.
Description
Technical Field
The invention relates to the technical field of aquaculture, in particular to an artificial breeding method of takifugu obscurus.
Background
The name of Chinese: d, takifugu obscurus; the name of English: porcupine stuffing; latin learning name: diodon hystrix. The Fugu obscurus is fish of Fugu, Fugu order, Fugu family. Distributed in various subtropical ocean areas around the world. The model origin of this species is in india. When attacked, the sea water can be sucked in to swell the body into a spherical shape, and thorns are erected to prevent enemies from swallowing. Carnivorous, which is a staple food of benthic animals and toxic. The body length of the takifugu obscurus can reach 91 cm. The body is short and wide, the mouth is small, and the upper jaw and the lower jaw in the mouth are respectively provided with a developed toothed plate. Except the lip and the caudal peduncle, strong spines specialized by scales are densely distributed. Short thorns and small black spots on the dense part of the body; each fin is short and round, and has small black dots, 14 to 17 soft strips of the dorsal fins; 14 to 16 hip fin soft strips.
The takifugu obscurus inhabits sand stone or gravel substrate sea area and has water depth of 2-30 m. The swimming speed is slow, the underwater robot is often hidden in a reef cave, seawater is sucked to swell the body into a spherical shape when the underwater robot is attacked, and thorns are erected to prevent enemies from swallowing. Carnivorous, which takes benthonic animals as staple food.
The takifugu obscurus seen in the market at present is mainly artificially caught. The meat is fresh, tender and delicious, has high nutritive value, larger individual, good market prospect and larger breeding and development prospect. However, the artificial propagation technology of the takifugu obscurus is not reported.
Disclosure of Invention
Therefore, the invention provides an artificial breeding method of takifugu obscurus, which solves the technical problem.
The technical scheme of the invention is realized as follows: an artificial breeding method of takifugu obscurus comprises the following steps:
s1, parent fish source and screening:
the parent fish of the takifugu obscurus is obtained by artificial domestication after being captured in a natural sea area, and the takifugu obscurus with the body mass of more than 3000g, the body length of more than 40cm, normal body shape, no injury, no disease and good activity is selected as a parent;
s2, intensive cultivation of parent fish:
the cultivation density is that the volume of an indoor parent fish cultivation pool is 6.0m × 6.0.0 m × 1.5.5 m, 2 diagonal water inlets and 1 drainage outlet are arranged, the cultivation density is 80-100 fish/pool, and the ratio of male and female is 1: 0.5-1;
the water quality requirement is as follows: regularly detecting temperature, salinity, dissolved oxygen, pH value, oxygenation power, ammonia nitrogen and nitroso nitrogen every day, keeping various water quality indexes of the water for cultivation to meet the requirements of cultivation conditions, wherein the salinity is 20-32 per mill, the temperature is 26-30 ℃, the pH is 7.5-8.6, and the dissolved oxygen is kept>5mg/L, requiring running water for breeding, keeping the water transparency above 50cm, and increasing the oxygen power by 1.5-2.5 w/m2The ammonia nitrogen is less than or equal to 0.5mg/L, and the nitroso nitrogen is less than or equal to 0.05 mg/L.
Feeding management: during the intensive cultivation of the parent fish, feeding twice at fixed points every day, wherein baits are fresh and live shrimps and razor clams, feeding after full feeding is carried out, water and dirt are changed and absorbed every day at about 16:00 in the afternoon, the shrimps and the razor clams are soaked for 12 hours by 0.5ppm of copper sulfate every 7 days, water is changed for 100 percent after 12 hours, then the parent fish flows at the speed of 50-80L/min, 2 days of medicine is continuously used every time, and the activity condition of the parent fish is observed every day.
S3, spawning induction of parent fish:
checking and judging the parent fish gonad development degree: after intensive cultivation of parent fish for 3 weeks, selecting partial individuals from parent fish groups every week to perform maturity inspection, selecting individuals with raised abdomen and reddish and protruded reproductive pores, inspecting on an operation platform, pushing the individuals from the abdomen to the reproductive pores by hands, and discharging white semen or transparent eggs to achieve sexual maturity.
Artificial hastening parturition: ripening and induced spawning are carried out according to the gonad development degree, the induced spawning medicines comprise diosdone, ovulation-promoting hormone No. 3 and chorionic gonadotropin, the injection dosage is 1-2.5 mg/kg of diosdone, 2.0-5 mug/kg of ovulation-promoting hormone No. 3 and 600-1000 IU/kg of chorionic gonadotropin, the injection dosage of male fishes is reduced by half or is not injected, the induced spawning time is 15: 00-16: 00 in the afternoon, the effect time is 39-49 h, and artificial induced spawning and natural insemination are combined.
S4, collecting and hatching fertilized eggs
After spawning, collecting fertilized eggs by using a soft bolting silk net of 80 meshes, repeatedly cleaning the collected fertilized eggs by using fresh seawater and removing impurities and dead eggs, putting the cleaned fertilized eggs into a bucket with 1.0m3 seawater, then adding 2ppm formaldehyde for sterilization for 5 minutes, continuously inflating the bucket during sterilization, fishing out the fertilized eggs by using the soft net of 80 meshes after the sterilization time reaches a certain point, flushing the fertilized eggs for 2-3 times by using clean seawater after fishing out, then gently putting the fertilized eggs into a prepared indoor hatching pond (3.0m × 3.0.0 m × 1.0m) for hatching, hatching the fertilized eggs out of a membrane after about 126 hours under the conditions of water temperature of 26.0-28.0 ℃ and salinity of 30.0-32.0, and transferring the newly hatched fish to a seedling pond for cultivation, wherein the total length of the newly hatched fish is about 3.3 mm.
Further, the incubation conditions comprise that the illumination intensity is less than or equal to 3000lux, the water temperature is 26.0-28.0 ℃, the salinity is 30.0-32.0, the pH value is 7.5-8.6, and the dissolved oxygen is more than or equal to 5 mg/L.
Compared with the prior art, the invention has the beneficial effects that:
(1) the artificial breeding method of the invention optimizes the fresh and alive shrimps and razor clams for the parent breeding of the takifugu obscurus according to the natural habit of the wild takifugu obscurus, so that the wild parent fish can adapt to the artificial domestication environment more quickly and better, and the gonad maturation speed is faster than that in the natural sea area.
(2) And the parent can make gonad mature under the condition of artificial cultivation by the coordination of technological conditions in temperature, salinity, dissolved oxygen, pH value, oxygenation power, ammonia nitrogen and nitroso nitrogen, and a large amount of fertilized eggs are obtained for artificial incubation by artificial induced spawning incubation. The parent fish has high spawning rate, high fertilized egg rate and hatching rate of fertilized eggs, and good newly hatched fry activity, and can meet the requirement of large-scale cultivation of takifugu obscurus fries.
Detailed Description
In order to better understand the technical content of the invention, specific examples are provided below to further illustrate the invention.
The experimental methods used in the examples of the present invention are all conventional methods unless otherwise specified.
The materials, reagents and the like used in the examples of the present invention can be obtained commercially without specific description.
Example 1
An artificial breeding method of takifugu obscurus comprises the following steps:
s1, parent fish source and screening:
the parent fish is purchased from Takifugu obscurus caught by fishermen in the west sand sea area, and the individuals with the body mass of more than 3000g, the body length of more than 40cm, normal body shape, no injury, no disease and good vitality are selected.
S2, intensive cultivation of parent fish:
the cultivation density is that the indoor parent fish cultivation pool has the volume of 6.0m × 6.0.0 m × 1.5.5 m, 2 diagonal water inlets and 1 drainage outlet are arranged, the cultivation density is 80 fish/pool, and the ratio of male and female is 1: 0.5;
the water quality requirement is as follows: regularly detecting temperature, salinity, dissolved oxygen, pH value, oxygenation power, ammonia nitrogen and nitroso nitrogen every day, keeping various water quality indexes of the water for cultivation to meet the requirements of cultivation conditions, wherein the salinity is 20-32 per mill, the temperature is 26-30 ℃, the pH is 7.5-8.6, and the dissolved oxygen is kept>5mg/L, requiring running water for breeding, keeping water transparency above 50cm, and increasing oxygenThe power is 1.5-2.5 w/m2The ammonia nitrogen is less than or equal to 0.5mg/L, and the nitroso nitrogen is less than or equal to 0.05 mg/L;
feeding management: during the intensive cultivation of the parent fish, feeding twice at fixed points every day, wherein baits are fresh and live shrimps and razor clams, feeding after full feeding is carried out, water and dirt are changed and absorbed every day at about 16:00 in the afternoon, the shrimps and the razor clams are soaked for 12 hours by 0.5ppm of copper sulfate every 7 days, water is changed for 100 percent after 12 hours, then the parent fish flows at the speed of 50-80L/min, 2 days of medicine is continuously used every time, and the activity condition of the parent fish is observed every day.
S3, spawning induction of parent fish:
checking and judging the parent fish gonad development degree: after intensive cultivation of parent fish for 3 weeks, selecting partial individuals from parent fish groups every week to perform maturity inspection, selecting individuals with raised abdomen and reddish and protruded reproductive pores, inspecting on an operation platform, pushing the individuals from the abdomen to the reproductive pores by hands, and discharging white semen or transparent eggs to achieve sexual maturity.
Artificial hastening parturition: ripening and oxytocic are carried out according to the gonad development degree, the oxytocic uses diosdone, ovulation-promoting hormone No. 3 and chorionic gonadotropin, the injection dosage is 1mg/kg of diosdone, 2.0 mu g/kg of ovulation-promoting hormone No. 3 and 600IU/kg of chorionic gonadotropin, the injection dosage of male fish is reduced by half or not injected, the oxytocic time is 15: 00-16: 00 in the afternoon, and the effect time is 39 h.
S4, collecting and hatching fertilized eggs
After spawning, collecting fertilized eggs by using a soft bolting silk net of 80 meshes, repeatedly cleaning the collected fertilized eggs by using fresh seawater and removing impurities and dead eggs, putting the cleaned fertilized eggs into a bucket with 1.0m3 seawater, adding 2ppm formaldehyde for sterilization for 5 minutes, continuously inflating the bucket during sterilization, fishing out the fertilized eggs by using the soft net of 80 meshes after the sterilization time reaches a certain point, flushing the fertilized eggs for 2-3 times by using the clean seawater after fishing out the fertilized eggs, then gently putting the fertilized eggs into a prepared indoor hatching pond (3.0m × 3.0.0 m × 1.0m) for hatching, hatching the fertilized eggs to form a membrane after 129 hours, and transferring the newly hatched fish into a seedling pond for cultivation when the total length of the newly hatched fish is about 3.3 mm.
The artificial incubation culture conditions comprise illumination intensity of 1000lux, water temperature of 26 ℃, pH value of 7.5, salinity of 30 and dissolved oxygen of 5 mg/L.
Example 2
An artificial breeding method of takifugu obscurus comprises the following steps:
s1, parent fish source and screening:
the parent fish is purchased from Takifugu obscurus caught by fishermen in the west sand sea area, and the individuals with healthy constitution, no disease and no trauma are screened, wherein the body mass is more than 3000g, and the body length is more than 40 cm.
S2, intensive cultivation of parent fish:
the cultivation density is that the indoor parent fish cultivation pool has the volume of 6.0m × 6.0.0 m × 1.5.5 m, 2 diagonal water inlets and 1 drainage outlet are arranged, the cultivation density is 100 fish/pool, and the ratio of male and female is 1: 0.8;
the water quality requirement is as follows: regularly detecting temperature, salinity, dissolved oxygen, pH value, oxygenation power, ammonia nitrogen and nitroso nitrogen every day, keeping various water quality indexes of the water for cultivation to meet the requirements of cultivation conditions, wherein the salinity is 20-32 per mill, the temperature is 26-30 ℃, the pH is 7.5-8.6, and the dissolved oxygen is kept>5mg/L, requiring running water for breeding, keeping the water transparency above 50cm, and increasing the oxygen power by 1.5-2.5 w/m2The ammonia nitrogen is less than or equal to 0.5mg/L, and the nitroso nitrogen is less than or equal to 0.05 mg/L;
feeding management: during the intensive cultivation of the parent fish, feeding twice at fixed points every day, wherein baits are fresh and live shrimps and razor clams, feeding after full feeding is carried out, water and dirt are changed and absorbed every day at about 16:00 in the afternoon, the shrimps and the razor clams are soaked for 12 hours by 0.5ppm of copper sulfate every 7 days, water is changed for 100 percent after 12 hours, then the parent fish flows at the speed of 50-80L/min, 2 days of medicine is continuously used every time, and the activity condition of the parent fish is observed every day.
S3, spawning induction of parent fish:
checking and judging the parent fish gonad development degree: after intensive cultivation of parent fish for 3 weeks, selecting partial individuals from parent fish groups every week to perform maturity inspection, selecting individuals with raised abdomen and reddish and protruded reproductive pores, inspecting on an operation platform, pushing the individuals from the abdomen to the reproductive pores by hands, and discharging white semen or transparent eggs to achieve sexual maturity.
Artificial hastening parturition: ripening and oxytocic are carried out according to the gonad development degree, the oxytocic uses diosdone, ovulation-promoting hormone No. 3 and chorionic gonadotropin, the injection dosage is 2.5mg/kg of diosdone, 5 mu g/kg of ovulation-promoting hormone No. 3 and 1000IU/kg of chorionic gonadotropin, the injection dosage of male fish is reduced by half or not injected, the oxytocic time is 15: 00-16: 00 in the afternoon, and the effect time is 49 h.
S4, collecting and hatching fertilized eggs:
after spawning, collecting fertilized eggs by using a soft bolting silk net of 80 meshes, repeatedly cleaning the collected fertilized eggs by using fresh seawater and removing impurities and dead eggs, putting the cleaned fertilized eggs into a bucket with 1.0m3 seawater, adding 2ppm formaldehyde for sterilization for 5 minutes, continuously inflating the bucket during the sterilization, fishing out the fertilized eggs by using the soft net of 80 meshes after the sterilization time reaches a certain point, flushing the fertilized eggs for 2-3 times by using the clean seawater after fishing out the fertilized eggs, then gently putting the fertilized eggs into a prepared indoor hatching pond (3.0m × 3.0.0 m × 1.0.0 m) for hatching, hatching the fertilized eggs to form a membrane after 120 hours, and transferring the newly hatched fish into a seedling raising pond for culturing when the total length of the newly hatched fish is about 3.3 mm.
The artificial incubation conditions comprise illumination intensity of 3000lux, water temperature of 28 ℃, pH value of 8.4, salinity of 32 and dissolved oxygen of 6 mg/L.
Example 3
An artificial breeding method of takifugu obscurus comprises the following steps:
s1, parent fish source and screening:
the parent fish is purchased from Takifugu obscurus caught by fishermen in the west sand sea area, and the individuals with healthy constitution, no disease and no trauma are screened, wherein the body mass is more than 3000g, and the body length is more than 40 cm.
S2, intensive cultivation of parent fish:
the cultivation density is that the volume of an indoor parent fish cultivation pool is 6.0m × 6.0.0 m × 1.5.5 m, 2 diagonal water inlets and 1 drainage outlet are arranged, the cultivation density is 80-100 fish/pool, and the ratio of male and female is 1: 1;
the water quality requirement is as follows: regularly detecting temperature, salinity, dissolved oxygen, pH value, oxygenation power, ammonia nitrogen and nitroso nitrogen every day, keeping various water quality indexes of the water for cultivation in accordance with the requirements of cultivation conditions, wherein the salinity is 20-32 per mill, and the temperature is 20-32 per mill26 to 30 ℃, pH7.5 to 8.6, and dissolved oxygen retention>5mg/L, requiring running water for breeding, keeping the water transparency above 50cm, and increasing the oxygen power by 1.5-2.5 w/m2The ammonia nitrogen is less than or equal to 0.5mg/L, and the nitroso nitrogen is less than or equal to 0.05 mg/L;
feeding management: during the intensive cultivation of the parent fish, feeding twice at fixed points every day, wherein baits are fresh and live shrimps and razor clams, feeding after full feeding is carried out, water and dirt are changed and absorbed every day at about 16:00 in the afternoon, the shrimps and the razor clams are soaked for 12 hours by 0.5ppm of copper sulfate every 7 days, water is changed for 100 percent after 12 hours, then the parent fish flows at the speed of 50-80L/min, 2 days of medicine is continuously used every time, and the activity condition of the parent fish is observed every day.
S3, spawning induction of parent fish:
checking and judging the parent fish gonad development degree: after 2 weeks of intensive cultivation of the parent fish, selecting partial individuals from parent fish groups every week to perform maturity inspection, selecting the individuals with raised abdomen and reddish and protruded genital pore, inspecting on an operation platform, and pushing the individuals from the abdomen to the genital pore by hands, so that white semen or transparent eggs can flow out and reach sexual maturity.
Artificial hastening parturition: ripening and oxytocic are carried out according to the gonad development degree, the oxytocic uses diosdone, ovulation-promoting hormone No. 3 and chorionic gonadotropin, the injection dosage is 2.0mg/kg of diosdone, the ovulation-promoting hormone No. 3.5 mu g/kg and the chorionic gonadotropin 800IU/kg, the injection amount of male fish is reduced by half or not, the oxytocic time is 15: 00-16: 00 in the afternoon, and the effect time is 45 h.
S4, collecting and hatching fertilized eggs:
after spawning, collecting fertilized eggs by using a soft bolting silk net of 80 meshes, repeatedly cleaning the collected fertilized eggs by using fresh seawater and removing impurities and dead eggs, putting the cleaned fertilized eggs into a bucket with 1.0m3 seawater, adding 2ppm formaldehyde for sterilization for 5 minutes, continuously inflating the bucket during the sterilization, fishing out the fertilized eggs by using the soft net of 80 meshes after the sterilization time reaches a certain point, flushing the fertilized eggs for 2-3 times by using the clean seawater after fishing out the fertilized eggs, then gently putting the fertilized eggs into a prepared indoor hatching pond (3.0m × 3.0.0 m × 1.0m) for hatching, hatching the fertilized eggs to form a membrane after 126 hours, and transferring the newly hatched fish into a seedling pond for cultivation when the total length of the newly hatched fish is about 3.3 mm.
The artificial incubation conditions comprise illumination intensity of 3000lux, water temperature of 27 ℃, pH value of 8.2, salinity of 30 and dissolved oxygen of 7 mg/L.
Comparative example 1
The present comparative example is different from example 3 in that the artificial breeding method of takifugu obscurus uses frozen crabs and small trash fish as baits in the step of intensive cultivation of parent fish of S2.
Comparative example 2
The difference between the comparative example and the example 3 is that in the artificial breeding method of the takifugu obscurus, illumination intensity is 3500lux, water temperature is 32 ℃, pH is 8.8, salinity is 32, and dissolved oxygen is 4mg/L in S4 artificial incubation.
Determination of results
The present invention makes statistics on the hatchability, survival rate, and other indicators of the takifugu obscurus fries obtained in examples 1 to 3 and comparative examples 1 to 2, and the results are shown in table 1.
| Group of | Fertilization Rate (%) | Hatching rate (%) |
| Example 1 | 70 | 80 |
| Example 2 | 72 | 83 |
| Example 3 | 76 | 87 |
| Comparative example 1 | 50 | 70 |
| Comparative example 2 | 47 | 71 |
The artificial breeding method of the takifugu obscurus provided by the invention coordinates the technological conditions of regulating and controlling temperature, salinity, dissolved oxygen, pH value, oxygenation power, ammonia nitrogen and nitroso nitrogen with the baits with scientific proportion, so that the parents can mature gonads under the condition of artificial cultivation, and a large amount of fertilized eggs are obtained for artificial incubation through artificial induced spawning and incubation. The parent fish has high spawning rate, high fertilized egg rate and hatching rate of fertilized eggs, and good newly hatched fry activity, and can meet the requirement of large-scale cultivation of takifugu obscurus fries.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (5)
1. An artificial breeding method of takifugu obscurus, which is characterized in that: the method comprises the following steps: parent fish source and screening, parent fish intensive cultivation, parent fish spawning induction and artificial incubation;
(1) parent fish source and screening
The parent fish of the takifugu obscurus is obtained by artificial domestication after being captured in a natural sea area, and an individual with the body mass of more than 3000g, the body length of more than 40cm, normal body shape, no injury, no disease and good activity is selected as a parent;
(2) intensive cultivation of parent fish
Feeding the parent fish twice at fixed time at fixed points every day during intensive cultivation of the parent fish, wherein baits are fresh and live shrimps and razor clams, the parent fish is fed after being saturated, water and dirt are changed and absorbed every day at 16:00 pm, the parent fish is soaked for 12 hours by 0.5ppm of copper sulfate every 7 days, water is changed for 100 percent after 12 hours, then the parent fish flows at the speed of 50-80L/min, and medicines are continuously used for 2 days; observing the activity condition of the parent fish every day, wherein the temperature of parent fish culture water is 26-30 ℃, the pH value is 7.5-8.6, and the dissolved oxygen is kept to be more than 5 mg/L;
(3) spawning induction of parent fish
Checking and judging the parent fish gonad development degree: after intensive cultivation of parent fish for 3 weeks, selecting partial individuals from parent fish groups every week for maturity inspection, selecting the individuals with raised abdomen and reddish and protruded genital pore, inspecting on an operation platform, pushing from the abdomen to the genital pore by hand to allow white semen or transparent egg grains to flow out to reach sexual maturity,
artificial hastening parturition: ripening and hastening parturition according to the gonad development degree, wherein the hastening parturition medicines comprise diosdone, ovulation-promoting hormone No. 3 and chorionic gonadotropin, the hastening parturition time is 15: 00-16: 00, and the effect time is 39-49 h;
(4) fertilized egg collection and incubation
After spawning, collecting fertilized eggs by using a soft bolting silk net of 80 meshes; repeatedly cleaning the collected fertilized eggs with fresh seawater and removing impurities and dead eggs; putting the clean fertilized eggs into the fertilized eggs added with 1.0m3Adding 2ppm formaldehyde into a seawater bucket for sterilization for 5 minutes, continuously inflating the bucket during sterilization, fishing fertilized eggs out in time by using a soft net of 80 meshes after sterilization, flushing the fertilized eggs for 2-3 times by using clean seawater after fishing out, then putting the fertilized eggs into an indoor hatching pond with the volume of 3.0m × 3.0.0 m × 1.0.0 m for hatching, hatching the fertilized eggs to form a membrane after hatching for about 126 hours under the conditions that the water temperature is 26.0-28.0 ℃ and the salinity is 30.0-32.0, wherein the total length of the newly hatched fries is about 3.3mm, and the newly hatched fries can be transferred into a seedling raising pond for cultivation.
2. The artificial breeding method of takifugu obscurus as claimed in claim 1, wherein the artificial breeding method comprises the following steps:
and (3) intensive cultivation of the parent fish:
the cultivation density is that the volume of an indoor parent fish cultivation pool is 6.0m × 6.0.0 m × 1.5.5 m, 2 diagonal water inlets and 1 drainage outlet are arranged, the cultivation density is 80-100 fish/pool, and the ratio of male and female is 1: 0.5-1;
the water quality requirement is as follows: regularly detecting temperature, salinity, dissolved oxygen, pH value, oxygenation power, ammonia nitrogen and nitroso nitrogen every day, keeping various water quality indexes of the water for cultivation to meet the requirements of cultivation conditions, wherein the salinity is 20-32 per mill, the temperature is 26-30 ℃, the pH is 7.5-8.6, and the dissolved oxygen is kept>5mg/L, requiring running water for breeding, keeping the water transparency above 50cm, and increasing the oxygen power by 1.5-2.5 w/m2The ammonia nitrogen is less than or equal to 0.5mg/L, and the nitroso nitrogen is less than or equal to 0.05 mg/L;
feeding management: feeding the parent fish twice at fixed time at fixed points every day during intensive cultivation of the parent fish, wherein baits are fresh and live shrimps and razor clams, the parent fish is fed after being saturated, water and dirt are changed and absorbed every day at about 16:00 pm, the parent fish is soaked for 12 hours by 0.5ppm of copper sulfate every 7 days, water is changed for 100 percent after 12 hours, then the parent fish flows at the speed of 50-80L/min, and medicines are continuously used for 2 days each time; the activity of the parent fish was observed every day.
3. The artificial breeding method of takifugu obscurus as claimed in claim 1, wherein the artificial breeding method comprises the following steps: the bait feeding amount in the parent fish intensive cultivation is 2.0-3.0% of the weight of the parent fish.
4. The artificial breeding method of takifugu obscurus as claimed in claim 1, wherein the artificial breeding method comprises the following steps: the spawning induction of the parent fish: and (3) injecting oxytocin, wherein the oxytocin comprises diosdone, ovulation-promoting hormone No. 3 and chorionic gonadotropin, the injection dosage is 1-2.5 mg/kg of diosdone, 2.0-5 mu g/kg of ovulation-promoting hormone No. 3 and 600-1000 IU/kg of chorionic gonadotropin, and the injection dosage of male fish is halved or not injected according to parent fish.
5. The artificial breeding method of takifugu obscurus as claimed in claim 1, wherein the artificial breeding method comprises the following steps: the incubation conditions comprise that the illumination intensity is less than or equal to 3000lux, the water temperature is 26.0-28.0 ℃, the salinity is 30.0-32.0, the pH is 7.5-8.6, and the dissolved oxygen is more than or equal to 5 mg/L.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111758617A (en) * | 2020-07-08 | 2020-10-13 | 海南热带海洋学院 | Indoor cement pond artificial breeding method for takifugu obscurus |
| CN113317241A (en) * | 2021-06-09 | 2021-08-31 | 中国水产科学研究院东海水产研究所 | Method for gonadotropic development and artificial induced spawning of parent fish of takifugu hexamaculatus |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108077116A (en) * | 2017-12-08 | 2018-05-29 | 中国水产科学研究院东海水产研究所 | A kind of artificial raise seedling method of wild six spots thorn Puffer |
| CN109819913A (en) * | 2019-03-26 | 2019-05-31 | 海南晨海水产有限公司 | A kind of artificial breeding method of Porcupinefiss |
-
2020
- 2020-04-26 CN CN202010336498.4A patent/CN111316940A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108077116A (en) * | 2017-12-08 | 2018-05-29 | 中国水产科学研究院东海水产研究所 | A kind of artificial raise seedling method of wild six spots thorn Puffer |
| CN109819913A (en) * | 2019-03-26 | 2019-05-31 | 海南晨海水产有限公司 | A kind of artificial breeding method of Porcupinefiss |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111758617A (en) * | 2020-07-08 | 2020-10-13 | 海南热带海洋学院 | Indoor cement pond artificial breeding method for takifugu obscurus |
| CN113317241A (en) * | 2021-06-09 | 2021-08-31 | 中国水产科学研究院东海水产研究所 | Method for gonadotropic development and artificial induced spawning of parent fish of takifugu hexamaculatus |
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