CN108770741A - A kind of hatching method of east star spot - Google Patents
A kind of hatching method of east star spot Download PDFInfo
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- CN108770741A CN108770741A CN201810498731.1A CN201810498731A CN108770741A CN 108770741 A CN108770741 A CN 108770741A CN 201810498731 A CN201810498731 A CN 201810498731A CN 108770741 A CN108770741 A CN 108770741A
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- 230000012447 hatching Effects 0.000 title claims abstract description 27
- 238000000034 method Methods 0.000 title claims abstract description 15
- 235000013601 eggs Nutrition 0.000 claims abstract description 13
- 238000011534 incubation Methods 0.000 claims abstract description 11
- 201000010099 disease Diseases 0.000 claims abstract description 5
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 5
- 230000000366 juvenile effect Effects 0.000 claims abstract description 4
- 238000012216 screening Methods 0.000 claims abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 45
- 241000251468 Actinopterygii Species 0.000 claims description 20
- 102000002322 Egg Proteins Human genes 0.000 claims description 14
- 108010000912 Egg Proteins Proteins 0.000 claims description 14
- 210000004681 ovum Anatomy 0.000 claims description 14
- 108700012941 GNRH1 Proteins 0.000 claims description 9
- 239000000579 Gonadotropin-Releasing Hormone Substances 0.000 claims description 9
- 239000005556 hormone Substances 0.000 claims description 9
- 229940088597 hormone Drugs 0.000 claims description 9
- 230000002196 ecbolic effect Effects 0.000 claims description 7
- 241000894006 Bacteria Species 0.000 claims description 6
- ODINCKMPIJJUCX-UHFFFAOYSA-N Calcium oxide Chemical compound [Ca]=O ODINCKMPIJJUCX-UHFFFAOYSA-N 0.000 claims description 6
- 235000013305 food Nutrition 0.000 claims description 5
- 210000004907 gland Anatomy 0.000 claims description 5
- 230000001568 sexual effect Effects 0.000 claims description 5
- 238000009360 aquaculture Methods 0.000 claims description 4
- 244000144974 aquaculture Species 0.000 claims description 4
- 230000026109 gonad development Effects 0.000 claims description 4
- 239000000292 calcium oxide Substances 0.000 claims description 3
- 235000012255 calcium oxide Nutrition 0.000 claims description 3
- 230000018109 developmental process Effects 0.000 claims description 3
- 230000000857 drug effect Effects 0.000 claims description 3
- 235000021323 fish oil Nutrition 0.000 claims description 3
- 230000036541 health Effects 0.000 claims description 3
- 238000005286 illumination Methods 0.000 claims description 3
- 239000012535 impurity Substances 0.000 claims description 3
- 230000001788 irregular Effects 0.000 claims description 3
- 230000013011 mating Effects 0.000 claims description 3
- 230000035800 maturation Effects 0.000 claims description 3
- 210000003205 muscle Anatomy 0.000 claims description 3
- 210000001672 ovary Anatomy 0.000 claims description 3
- 239000011782 vitamin Substances 0.000 claims description 3
- 229940088594 vitamin Drugs 0.000 claims description 3
- 238000004140 cleaning Methods 0.000 claims 1
- 150000001875 compounds Chemical class 0.000 claims 1
- 230000008034 disappearance Effects 0.000 claims 1
- 238000006213 oxygenation reaction Methods 0.000 claims 1
- 239000002504 physiological saline solution Substances 0.000 claims 1
- 239000010802 sludge Substances 0.000 claims 1
- 230000004083 survival effect Effects 0.000 abstract description 2
- 230000000052 comparative effect Effects 0.000 description 13
- 238000002347 injection Methods 0.000 description 4
- 239000007924 injection Substances 0.000 description 4
- 241000238557 Decapoda Species 0.000 description 2
- 238000005276 aerator Methods 0.000 description 2
- 229960004407 chorionic gonadotrophin Drugs 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000017448 oviposition Effects 0.000 description 2
- 230000032696 parturition Effects 0.000 description 2
- 230000035479 physiological effects, processes and functions Effects 0.000 description 2
- 230000001007 puffing effect Effects 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 235000015170 shellfish Nutrition 0.000 description 2
- 241000238366 Cephalopoda Species 0.000 description 1
- 241000269799 Perca fluviatilis Species 0.000 description 1
- 241000321455 Plectropomus leopardus Species 0.000 description 1
- 241001417495 Serranidae Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000000686 essence Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 244000062645 predators Species 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- GOLXNESZZPUPJE-UHFFFAOYSA-N spiromesifen Chemical compound CC1=CC(C)=CC(C)=C1C(C(O1)=O)=C(OC(=O)CC(C)(C)C)C11CCCC1 GOLXNESZZPUPJE-UHFFFAOYSA-N 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/17—Hatching, e.g. incubators
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The present invention proposes a kind of hatching method of eastern star spot, including：1 Juvenile stage, 2 reinforced cultivatings, 3 artificial induced spawnings, the screening of 4 fertilized eggs is collected, 5 hatcheries prepare, 6 artificial incubation.A kind of eastern star spot hatching method provided by the invention; the hatching survival rate of eastern star spot can be increased to 98.6%; far above Natural Hatching Rate, and the eastern star spot fry quality hatched is good, and deformity is few; reform of nature growing environment; disease immune ability is strong, fast growing, and color is gorgeous; eastern star spot can be preferably protected, while generating economic value.
Description
Technical field
The present invention relates to technical field of aquaculture, and in particular to a kind of hatching method of east star spot.
Background technology
Eastern star spot, Plectropomus leopardus (Lacepede, a kind of fish that 1802) , Sushi sections gill spine perch belong to.
Eastern star spot is bright-colored, is well suited for raising the mixed breeding cylinder in violent fish, such as grouper.Lithosporic is very violent predator, meeting
Any fish that it can be gulped down is gulped down, can also eat up ornamental shrimp and other shellfish, but not harass them and can not gulp down any more
The fish gone can only fight with star spot.Eastern star spot food includes various ocean fishes, squid, shellfish and shrimp, needs often to provide some work
Food, enough nutrition can keep its color.Currently, in China, eastern star spot has been listed in shielded fish, and government is severe
Forbid people to catch and grab this fish, therefore eastern star spot has become the fish of rare variety.
Invention content
For mirror with this, it is an object of the invention to propose a kind of hatching method of eastern star spot.
The technical proposal of the invention is realized in this way：
A kind of hatching method of east star spot, includes the following steps：
Step 1, Juvenile stage：Selection health, build are normal well-balanced, disease-free, energetic, the good eastern star of gonad development
Spot is that the ratio of parent, wherein milter and raun are 1:2-3；
Step 2, reinforced cultivating：Selection wind sheltering condition is good, flow is unimpeded, depth of water 5m or more, marine site flow velocity be less than 0.2m/
Aquaculture net cage is arranged in the inner bay of s or shallow sea, and parent population reinforced cultivating is carried out before mating period；Artificial puffing cooperation is fed daily to raise
Material twice, at each 1 time of upper and lower noon, is satiated with food and is fed, and feed can add 0.15% fish oil and 1% multi-vitamins, promotes sexual gland hair
It educates；
Step 3, artificial induced spawning：The star spot raun development of ovary play the host to IV end of term, hastens parturition to the injection of eastern star spot adult fish dorsal muscles
Hormone, oxytocic hormone are luteinizing hormone releasing hormone LRH-A3 and human chorionic gonadtropin HCG；
Step 4, fertilized eggs screening are collected：Spawning pond for parent fish temperature is controlled at 22-25 degrees Celsius, when the parent population of sexual gland maturation
After oviposition, fish-egg is picked up per the fishing net of daily 50 mesh, separating tank is put into after collection, by impurity, dead ovum, unfertilized ovum and
It is excessively tiny, size is irregular, the fertilized eggs of dull remove, by the high quality fertilized egg of scophthatmus being collected into count after be put into it is ready
Hatchery；
Step 5, hatchery prepare：Preparing a round hatchery, diameter is in 2-5m, and depth of water 0.7-1.4m, bottom of pond is flat,
Fishpond clear up and rich water before putting seedling, the 3-5d before fry is launched and cultivates phytoplankton in water, after rich water daytime start
Aerator promotes phytoplankton growth；
Step 6, artificial incubation：High quality fertilized egg of scophthatmus is put into hatchery, control hatchery intensity of illumination in 500Lx hereinafter,
Artificial incubation is carried out under weak basic condition；
Further, in the step 3, oxytocic hormone is luteinizing hormone releasing hormone LRH-A3 and chorionic gonadotrophin swashs
Plain HCG, eastern star spot raun metering is LRH-A3 2.5-4.5ug/kg and HCG 900-1200U/kg, is dissolved in 3-4ml physiology salts
In water, it is injected into eastern star spot raun body using a shot；And simultaneously to milter injection luteinizing hormone releasing hormone LRH-A3
With human chorionic gonadtropin HCG, dosage halves.
Further, in the step 5, hatchery cleanup step is：Water was drained in 7-14 days before putting ovum, removes bottom
Portion's mud is exposed to the sun 3-5 days, water is then added to 8-12cm, using quick lime 0.8kg/m3Thorough clear pond waits for that drug effect disappears
Afterwards, new water is injected in hatchery；Rich water uses rich water king and viable bacteria, in the rich water king 0.4- that by 1m water gages, splashes per acre
0.7kg, then apply viable bacteria 0.6kg.
Further, in the step 6, incubation condition is：25-30 degrees Celsius of water temperature, salinity 26-30, pH value 8-
8.5；It is ten thousand/m of 35-50 to put ovum density3。
Compared with prior art, beneficial effects of the present invention are：The present invention provides a kind of eastern star spot hatching methods, can
The hatching survival rate of eastern star spot is increased to 98.6%, is far above Natural Hatching Rate, and hatches the eastern star spot fry quality come
Good, deformity is few, and reform of nature growing environment, disease immune ability is strong, fast growing, and color is gorgeous, can preferably protect east
Star spot, while generating economic value.
Specific implementation mode
For the technical solution in the present invention is clearly and completely described in conjunction with the embodiments, it is clear that inventor says
It is bright, but it is only a part of the embodiment of the present invention that following embodiment is described, instead of all the embodiments.Based on the present invention
In embodiment, every other implementation obtained by those of ordinary skill in the art without making creative efforts
Example, shall fall within the protection scope of the present invention.
A kind of hatching method of east star spot, includes the following steps：
Step 1, Juvenile stage：Selection health, build are normal well-balanced, disease-free, energetic, the good eastern star of gonad development
Spot is that the ratio of parent, wherein milter and raun are 1:2-3；
Step 2, reinforced cultivating：Selection wind sheltering condition is good, flow is unimpeded, depth of water 5m or more, marine site flow velocity be less than 0.2m/
Aquaculture net cage is arranged in the inner bay of s or shallow sea, and parent population reinforced cultivating is carried out before mating period；Artificial puffing cooperation is fed daily to raise
Material twice, at each 1 time of upper and lower noon, is satiated with food and is fed, and feed can add 0.15% fish oil and 1% multi-vitamins, promotes sexual gland hair
It educates；
Step 3, artificial induced spawning：The star spot raun development of ovary play the host to IV end of term, hastens parturition to the injection of eastern star spot adult fish dorsal muscles
Hormone, oxytocic hormone are luteinizing hormone releasing hormone LRH-A3 and human chorionic gonadtropin HCG；
Step 4, fertilized eggs screening are collected：Spawning pond for parent fish temperature is controlled at 22-25 degrees Celsius, when the parent population of sexual gland maturation
After oviposition, fish-egg is picked up per the fishing net of daily 50 mesh, separating tank is put into after collection, by impurity, dead ovum, unfertilized ovum and
It is excessively tiny, size is irregular, the fertilized eggs of dull remove, by the high quality fertilized egg of scophthatmus being collected into count after be put into it is ready
Hatchery；
Step 5, hatchery prepare：Preparing a round hatchery, diameter is in 2-5m, and depth of water 0.7-1.4m, bottom of pond is flat,
Fishpond clear up and rich water before putting seedling, the 3-5d before fry is launched and cultivates phytoplankton in water, after rich water daytime start
Aerator promotes phytoplankton growth；
Step 6, artificial incubation：High quality fertilized egg of scophthatmus is put into hatchery, control hatchery intensity of illumination in 500Lx hereinafter,
Artificial incubation is carried out under weak basic condition；
Further, in the step 3, oxytocic hormone is luteinizing hormone releasing hormone LRH-A3 and chorionic gonadotrophin swashs
Plain HCG, eastern star spot raun metering is LRH-A3 2.5-4.5ug/kg and HCG 900-1200U/kg, is dissolved in 3-4ml physiology salts
In water, it is injected into eastern star spot raun body using a shot；And simultaneously to milter injection luteinizing hormone releasing hormone LRH-A3
With human chorionic gonadtropin HCG, dosage halves.
Further, in the step 5, hatchery cleanup step is：Water was drained in 7-14 days before putting ovum, removes bottom
Portion's mud is exposed to the sun 3-5 days, water is then added to 8-12cm, using quick lime 0.8kg/m3Thorough clear pond waits for that drug effect disappears
Afterwards, new water is injected in hatchery；Rich water uses rich water king and viable bacteria, in the rich water king 0.4- that by 1m water gages, splashes per acre
0.7kg, then apply viable bacteria 0.6kg.
Further, in the step 6, incubation condition is：25-30 degrees Celsius of water temperature, salinity 26-30, pH value 8-
8.5；It is ten thousand/m of 35-50 to put ovum density3。
According to the hatching method of above-mentioned eastern star spot, table 1,2 given parameter of table hatch the east of embodiment 1-5, comparative example 1-5
Star spot.
Embodiment 1-5：
Table 1：
| Unit | Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | Embodiment 5 | |
| HCG | ug/kg | 2.5 | 3 | 3.5 | 4 | 4.5 |
| LRH-A3 | U/kg | 1200 | 1100 | 1000 | 900 | 1000 |
| Rich water king | kg | 0.5 | 0.4 | 0.6 | 0.7 | 0.5 |
| Put ovum density | Ten thousand/m3 | 45 | 30 | 50 | 40 | 45 |
| Hatch water temperature | Degree Celsius | 25 | 30 | 28 | 26 | 27 |
| Hatch PH | —— | 8 | 8.2 | 8.4 | 8.5 | 8.3 |
| Hatch salinity | —— | 28 | 30 | 26 | 27 | 29 |
Comparative example 1-5：
Table 2：
| Unit | Comparative example 1 | Comparative example 2 | Comparative example 3 | Comparative example 4 | Comparative example 5 | |
| HCG | ug/kg | 3 | 5 | 4 | 4.5 | 3 |
| LRH-A3 | U/kg | 800 | 1300 | 1100 | 1000 | 1000 |
| Rich water king | kg | 0.5 | 0.5 | 0.6 | 0.8 | 0.3 |
| Put ovum density | Ten thousand/m3 | 40 | 50 | 30 | 40 | 60 |
| Hatch water temperature | Degree Celsius | 23 | 26 | 29 | 32 | 30 |
| Hatch PH | —— | 8.8 | 8.5 | 8.2 | 7.8 | 8.1 |
| Hatch salinity | —— | 25 | 28 | 32 | 29 | 28 |
Hatch results are as shown in table 3, table 4, wherein young fish length measures on the 4th day after hatching：
Table 3：
| Unit | Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | Embodiment 5 | |
| Hatching rate | % | 96.9 | 98.1 | 97.4 | 98.6 | 97.5 |
| Brooding time | h | 21 | 17 | 19 | 20 | 19 |
| Young fish length | mm | 16.3 | 16.8 | 16.5 | 16.9 | 16.4 |
| The long deviation of body | mm | ±0.21 | ±0.16 | ±0.18 | ±0.16 | ±0.19 |
Table 4：
| Unit | Comparative example 1 | Comparative example 2 | Comparative example 3 | Comparative example 4 | Comparative example 5 | |
| Hatching rate | % | 83.2 | 93.3 | 92.6 | 89.7 | 93.5 |
| Brooding time | h | 34 | 24 | 20 | 16.5 | 17.5 |
| Young fish length | mm | 16.1 | 15.9 | 15.7 | 16.0 | 15.1 |
| The long deviation of body | mm | ±0.29 | ±0.26 | ±0.20 | ±0.22 | ±0.19 |
As can be seen that according to eastern star spot hatching method disclosed in this invention, hatching rate is 97.4% or more, young fish length
In 16.3mm or more, and body length is uniform, and brooding time is in 21 hours.Wherein, 4 given incubation condition of embodiment is most appropriate,
Hatching rate is up to 98.6%, and in fish length in 16.9mm, deviation is ± 0.16, and brooding time is in 20h.Beyond the given model of the present invention
It encloses, then the hatching rate of eastern star spot can be caused to reduce.By comparative example as can be seen that the relatively low influence that can be to brooding time of temperature most
Greatly, salinity and PH influence hatching rate maximum.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
With within principle, any modification, equivalent replacement, improvement and so on should all be included in the protection scope of the present invention god.
Claims (4)
1. a kind of hatching method of east star spot, which is characterized in that include the following steps：
Step 1, Juvenile stage：Selection health, build are normal well-balanced, disease-free, energetic, and the good eastern star spot of gonad development is
The ratio of parent, wherein milter and raun are 1:2-3；
Step 2, reinforced cultivating：It selects wind sheltering condition is good, flow is unimpeded, depth of water 5m or more, marine site flow velocity are less than 0.2m/s
Aquaculture net cage is arranged in inner bay or shallow sea, and parent population reinforced cultivating is carried out before mating period；Artificial expanding compound feed is fed daily
Twice, each 1 time of upper and lower noon, is satiated with food and feeds, and feed can add 0.15% fish oil and 1% multi-vitamins, promotes gonad development；
Step 3, artificial induced spawning：The star spot raun development of ovary play the host to IV end of term, oxytocic hormone is injected to eastern star spot adult fish dorsal muscles,
Oxytocic hormone is luteinizing hormone releasing hormone LRH-A3 and human chorionic gonadtropin HCG；
Step 4, fertilized eggs screening are collected：Spawning pond for parent fish temperature is controlled at 22-25 degrees Celsius, when the Gunther parent fish spawning of sexual gland maturation
Afterwards, fish-egg is picked up per the fishing net of daily 50 mesh, separating tank is put into after collection, by impurity, dead ovum, unfertilized ovum and excessively
It is tiny, size is irregular, the fertilized eggs of dull remove, will the high quality fertilized egg of scophthatmus that be collected into count after be put into ready hatching
Pond；
Step 5, hatchery prepare：Prepare a round hatchery, diameter is in 2-5m, and depth of water 0.7-1.4m, bottom of pond is flat, is putting
Fishpond clear up and rich water before seedling, the 3-5d before fry is launched and cultivates phytoplankton in water, after rich water daytime start oxygenation
Machine promotes phytoplankton growth；
Step 6, artificial incubation：High quality fertilized egg of scophthatmus is put into hatchery, control hatchery intensity of illumination is in 500Lx hereinafter, weak
Artificial incubation is carried out under alkaline condition.
2. the hatching method of east star spot according to claim 1, which is characterized in that in the step 3, oxytocic hormone is to promote
Luteinizing hormone releasing hormone LRH-A3 and human chorionic gonadtropin HCG, eastern star spot raun metering is LRH-A3 2.5-4.5ug/kg
It with HCG 900-1200U/kg, is dissolved in 3-4ml physiological saline, is injected into eastern star spot raun body using a shot；And
Luteinizing hormone releasing hormone LRH-A3 is injected to milter simultaneously and human chorionic gonadtropin HCG, dosage halve.
3. the hatching method of east star spot according to claim 1, which is characterized in that in the step 5, hatchery cleaning step
Suddenly it is：Water was drained in 7-14 days before putting ovum, removes bottom sludge, be exposed to the sun 3-5 days, water is then added into 8-12cm, is adopted
With quick lime 0.8kg/m3New water is injected in thorough clear pond after drug effect disappearance in hatchery；Rich water uses rich water king and work
Bacterium in the rich water king 0.4-0.7kg that by 1m water gages, splashes per acre, then applies viable bacteria 0.6kg.
4. the hatching method of east star spot according to claim 1, which is characterized in that in the step 6, incubation condition is：
25-30 degrees Celsius of water temperature, salinity 26-30, pH value 8-8.5；It is ten thousand/m of 35-50 to put ovum density3。
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114766396A (en) * | 2022-05-06 | 2022-07-22 | 广东越群生物科技股份有限公司 | Nutrition strengthening method for promoting development of young red east star spot fish |
| CN115152669A (en) * | 2022-07-15 | 2022-10-11 | 广东海洋大学 | Safe and effective east star spot red-increasing cultivation method |
| CN117796348A (en) * | 2024-01-30 | 2024-04-02 | 广东海洋大学 | Hatching net cage device for Dongxing spot family construction and application method thereof |
| CN118058213A (en) * | 2024-04-19 | 2024-05-24 | 中国海洋大学三亚海洋研究院 | Industrial indoor total artificial breeding method for leopard gill-acanthus japonicus |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114766396A (en) * | 2022-05-06 | 2022-07-22 | 广东越群生物科技股份有限公司 | Nutrition strengthening method for promoting development of young red east star spot fish |
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| CN115152669B (en) * | 2022-07-15 | 2024-04-09 | 广东海洋大学 | Safe and effective red-increasing cultivation method for east star spots |
| CN117796348A (en) * | 2024-01-30 | 2024-04-02 | 广东海洋大学 | Hatching net cage device for Dongxing spot family construction and application method thereof |
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