CN1116896A - Artificial fry culturing method for red-fin dongfang globe fish - Google Patents

Abstract

The said method adopts the technology of aquatic product culture. It features the combined conventional and ecological fry-culturing processes including mother fish catching and spawning, incubation of spawns and fry culturing. The said process for artificially culturing fry of red-fin Dongfang puffer brings about high survival rate and thus has high economic value.

Description

Artificial fry culturing method for red-fin dongfang globe fish

The invention discloses a kind of Fugu rubripes fish artificial raise seedling method, belong to technical field of aquaculture, be used for artificial culture Fugu rubripes fry, development marine culture and stock enhancement industry.

Along with the continuous development of world industry mechanization until the electrification fishing, the increasing of catching intensity, ocean Mesichthyes natural resources constantly fails, and people more and more pay attention to the artificial increasing cultivation of seawater fish.Carry out the artificial increasing cultivation of seawater fish, just must solve the seed problem of marine culture and stock enhancement fish earlier.Flourishing Japan, Norway and the China of each fishery state, especially sea fishery of the world all successively carried out multiple marine fish artificial breeding test for this reason, and grey mullet,

Fish, salted cake fried in sesame oil fish, porgy etc. have begun to the transition of industrialization artificial breeding, but can really form large-scale industrialized grow seedlings also few in number, and especially China also rests on experimental stage basically." each opinion of pisciculture " that volume through consulting this letter of Japanese river was published in 1971 reaches by the end of the published patent gazette of present China Patent Office, all finds no the same or analogous technology contents with the present invention.

The object of the present invention is to provide that a kind of method is easy to learn, consistent, survival rate is high, equipment is simple, conventional method is grown seedlings with ecosystem and grow seedlings to combine the artificial fry culturing method for red-fin dongfang globe fish of to cultivate in enormous quantities that easy breed, economic worth are big, the high Takifugu rubripes of earning foreign exchange through export being taken aim at kind.

Biological property according to biological engineering's basic principle and Fugu rubripes fish, especially reproductive biology characteristic, at the subject matter that exists in the prior art, basic design of the present invention is: the utilization medicine technology of hastening the hatching of silkworms obtains the fertilized egg of Fugu rubripes, adopt the ecosystem seedling method then, be about to middle-size and small-size cement pit and be considered as an ecosystem among a small circle, the variation characteristics and the rule of various biotic factors and physical and chemical factor in correct this ecosystem of grasp, from the unified relation of contradiction between each factor of angle control of ecosystem balance, make incubating oosperm, prelarva, postlarva all can grow up to juvenile fish in normal development, guarantee whole survival rate, bring out healthy and strong seed.

The invention is characterized in that described artificial fry culturing method for red-fin dongfang globe fish comprises the steps: that adopting of (1) parent population catch, select and hasten parturition: annual Fugu rubripes between the egg-laying period from catching or the fisherman delivers to purchase spot and supports temporarily and select in the Fugu rubripes of the work the storage pond, holding pond of 1～3 cube of water body and inflation not have to hinder by marine adopting, vivaciously, swimming is normal, do parent population more than 2 kilograms, with have gentle hands press the live fish belly extrude seminal fluid for male, though extrude ovum or can not squeeze ovum but belly the greater is female, determine after the male and female promptly toward parent population dorsal fin and/or pectoral fin base portion injection ocyodinic, then by female: hero=1: 3 is put into spawning pond; (2) adopt the ovum hatching: the female parent population after hasten parturition above-mentioned (1), when pressing its belly to outbound flow ovum immediately with have gentle hands, promptly carry out the wet method artificial insemination, obtain to put into the hatching cylinder immediately after the fertilized egg and hatch, the ovum density of putting during hatching is every cube of water body 8～150,000,14 ℃～20 ℃ of water temperatures, hatching 10～6 days time decides with water temperature, and the water temperature height is hatched soon, should inflate in a large number continuously during this, in case the opaque fertilized egg of heavy property is sunk, they are suspended in water all the time; (3) prelarva is cultivated: putting into volume after prelarva hatches immediately is 3～5 cubes of water bodys, clean up in advance, sterilize with potassium permanganate, continuous wash is put clear water for 3～4 times and is treated in one day the prelarva cultivation pond again, under 0.5～1 meter condition of the depth of water, cultivate, breeding density is every cube of water body 1～20,000 tails, the hatching back was thrown in unit cell algae and wheel animalcule on the 3rd day in above-mentioned prelarva is cultivated the pond, wheel animalcule density is 5～10/ml, every cube of water body 40～600,000, and change water in conjunction with the clear end 2～3 time with siphonage daytime every day, follow bait throwing in after changing water at every turn, prelarva is raised total length 5mm when beginning to cut one another's throats, and in time carries out the branch pond, and breeding density changes every cube of water body 0.4～0.6 ten thousand tails into, bait throwing in every day 3～4 times, daily ration, feeding quantity is wanted sufficient and the fish meat emulsion of suitably throwing something and feeding, and prelarva is fully had enough, in case prelarva carries out residual mutually because of hunger; (4) postlarva is raised: above-mentioned prelarva was cultivated 20～30 days in prelarva is cultivated the pond, when total length reaches 7mm, 2000～2500 tails in every cubic metre of water body, except that continuing to throw something and feed the wheel animalcule with equal densities, the fish meat emulsion of throwing something and feeding simultaneously, and progressively change into based on the fish meat emulsion of throwing something and feeding, suitably add living baits such as copepoda, artemia nauplii; (5) juvenile fish is raised: above-mentioned postlarva was cultivated in the postlarva pond about 20 days, fish length reaches about 25mm, before selling fry, in the time of need continuing to raise as can not in time going out the pond, then bait adds bone meal and/or mineral matter and multivitamin and add right quantity brown alga to water colour in raising the pond with the flesh of fish and keeps pitchy, making becomes a small-sized ecosystem in the pond, to improve survival rate of fish fry, till fish length 25～30mm sells seedling.

Above-mentioned ocyodinic is that human chorionic gonadtropin (HCG) adds carp hypophysis, dosage is 1～2/kilogram of HCG1500～2500 international unit+hypophysis, if a shot is ineffective, carry out the injection second time after three days again, dosage changes HCG1000～1500 international unit/kilograms into.

Above-mentioned fish meat emulsion is for making of flesh of fish such as lean fish class such as sand lance, refrigerant fishes, its processing method be earlier large-scale fish-bone is rejected clean shine dry grinding after, together be stirred to separately in the fish meat emulsion that picks a bone that blends with meat mincer together with B B-complex and/or mineral matter powder.

Above-mentioned small-sized ecosystem, be meant from the above-mentioned fish meat emulsion of throwing something and feeding begin to fry is long sell to total length 25～30mm till, raise and remain at least 10～100 * 104 cells/ml unit cell algae and brown alga in the pond, make the pool inner water body become living baits such as fry and wheel animalcule, halogen worm and can carry out multiple biologies such as photosynthetic plant and the physical and chemical factor and the small-sized ecosystem of depositing, and regulate to keep this holocoenotic balance with instrument monitoring, so that reduce or prevent water pollution, guarantee the healthy all the time active growth of fry.

Above-mentioned juvenile fish is raised and can also at sea raise in the net cage in outdoor sink.

Outstanding advantage of the present invention is that method is easy to learn, the seedling-cultivating method that adopts conventional method and ecosystem to grow seedlings and combine, the Fugu rubripes that fry is a quality better, economic worth is high that the energy assurance brings out, with this method incubation rate height of growing seedlings, the survival rate of fish fry height that brings out, used equipment is simple, and cost is low, workable, consistent.

Embodiments of the invention are Fugu rubripes artificial breeding production tests that the inventor carried out in saltern, the Bohai Sea, Changyi, Shandong city in 1993.Method step as above-mentioned feature partly as described in, be from the 25 tail parent populations of catching for 5～June then, to select 5 kilograms of body weight at sea, 4 kilograms of each female parent populations of 4 tails, though can extrude fish-egg or can not squeeze fish-egg by its belly with have gentle hands, but when belly is soft, at its dorsal fin base portion HCG injection 2000 international units+2 carp hypophysis/kilograms, 1,500,000 in injection back coextrusion fish-egg, hatching is to carry out in volume is the circular glass cylinder barrel of 0.4 cube of water body, put 2 gas stone inflations in the tube, average fertilization rate is 70%, incubation rate is 75%, first to the 3rd day flat the trip in the water surface and nearly superficial water of prelarva in hatching back, the 4th day begins to absorb nourishment from the external world, at this moment 10/milliliter of wheel animalcule less thaies begin to throw something and feed, black cloth on the upper cover of prelarva pond, illumination is the 500-1000 lux on the maintenance water surface, pool volume is 30 cubes of water bodys during the about 2.6～2.9mm of prelarva total length, prelarva 200,000 tails are put in every pond, cultivated for 2～3 week like this, survival rate is more than 70%, and the prelarva total length reaches 5mm, copepoda and residual mutually begins to ingest, at this moment carry out the branch pond and move to outdoor raising, total length reaches 2.5～3 centimetres after 40 days, and coexistence is lived more than 170,000 tails in 230 cubes of water bodys.

Change as present embodiment:

1, in tolerance band, respectively there is related parameter all can suitably change;

2, the depth of various pond size, water, change waterside number, quantity of exchanged water size, inflation what etc. all can grasp flexibly;

The kind of the living bait of 3, throwing something and feeding, all can suitably change for the kind that forms the unit cell algae that ecosystem among a small circle need throw in.

Claims (5)

1, a kind of artificial fry culturing method for red-fin dongfang globe fish, hasten the hatching of silkworms and young postlarva is cultivated and forms by parent population, the invention is characterized in described artificial fry culturing method for red-fin dongfang globe fish, comprising the steps: that (1) parent population is adopted catches, selects and hasten parturition: annual Fugu rubripes is between the egg-laying period, by marine adopt catch select in the Fugu rubripes of supporting temporarily not have hinder, active, swimming is normal, body weight is done parent population more than 2 kilograms, behind female parent population dorsal fin and/or pectoral fin base portion injection ocyodinic, by female: hero=1: 3 is put into spawning pond; (2) adopt the ovum hatching: the female parent population after hasten parturition above-mentioned (1), when pressing its belly to outbound flow ovum immediately with have gentle hands, promptly carry out the wet method artificial insemination, obtaining fertilized egg puts into the hatching cylinder immediately and hatches, put ovum density and be every cube of water body 8～150,000,14～20 ℃ of water temperatures are hatched 10～6 days time, a large amount of continuously inflations suspend in water fertilized egg all the time; (3) prelarva is cultivated: putting into volume after prelarva hatches immediately is the prelarva cultivation pond of 3～5 cubes of water bodys, under 0.5～1 meter condition of the depth of water, cultivate, breeding density is every cube of water body 1～20,000 tails, the hatching back was thrown in unit cell algae and wheel animalcule on the 3rd day in above-mentioned prelarva is cultivated the pond, wheel animalcule density is 5～10/ml, every cube of water body 40～600,000, change water with siphonage daytime every day, in conjunction with the clear end 2～3 times, change then bait throwing in behind the water, when the prelarva total length reaches the mutual residual beginning of 5mm at every turn, promptly carry out the branch pond, breeding density changes every cube of water body 0.4～0.6 ten thousand tails into, bait throwing in every day 3～4 times, and the fish meat emulsion of suitably throwing something and feeding; (4) postlarva is raised: above-mentioned prelarva was cultivated 20～30 days in prelarva is cultivated the pond, when total length reaches 7mm, 2000～2500 tails in every cube of water body, except that continuing to throw something and feed the wheel animalcule with equal densities, the fish meat emulsion of throwing something and feeding simultaneously, and progressively change into based on the fish meat emulsion of throwing something and feeding, suitably add copepoda and/or artemia nauplii living bait; (5) juvenile fish is raised: above-mentioned postlarva was cultivated in the postlarva pond about 20 days, before fish length reaches and prepares about 25mm to sell fry, bait is that fish meat emulsion adds bone meal and/or mineral matter and multivitamin, and in raising the pond, add right quantity brown alga to water colour and keep pitchy, become a small-sized ecosystem in the pond, till fish length 25～30mm sells seedling.

2, want 1 described artificial fry culturing method for red-fin dongfang globe fish according to right, it is characterized in that above-mentioned ocyodinic is that human chorionic gonadtropin adds carp hypophysis, dosage is 1～2/kilogram of HCG1500～2500 international unit+hypophysis; Need twice of injection, carried out after three days for the second time, dosage changes HCG1000～1500 international unit/kilograms into.

3, artificial fry culturing method for red-fin dongfang globe fish according to claim 1, it is characterized in that above-mentioned fish meat emulsion for making of lean fish class such as sand lance, the refrigerant fish flesh of fish, its processing method is large-scale fish-bone to be cleaned with a pointed instrument to clean earlier together to be stirred in the fish meat emulsion that picks a bone that blends separately together with B B-complex and/or mineral matter powder after shining dry grinding.

4, artificial fry culturing method for red-fin dongfang globe fish according to claim 1, it is characterized in that above-mentioned small-sized ecosystem, be meant from the above-mentioned fish meat emulsion of throwing something and feeding begin to fry is long sell to total length 25～30mm till, raise and remain at least 10～100 * 10 in the pond ⁴Unit cell algae and the brown alga of cell/ml, water body are fry and wheel animalcule living bait and can carry out the multiple biology of photosynthetic plant and the physical and chemical factor and the small-sized ecosystem of depositing, and keep its balance with the instrument monitoring adjustment at any time.

5, artificial fry culturing method for red-fin dongfang globe fish according to claim 1 is characterized in that above-mentioned juvenile fish raises, and can also at sea raise in the net cage in outdoor sink.