CN107980670A - A kind of Pelteobagrus fulvidraco induced spawning method - Google Patents
A kind of Pelteobagrus fulvidraco induced spawning method Download PDFInfo
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- CN107980670A CN107980670A CN201711223864.XA CN201711223864A CN107980670A CN 107980670 A CN107980670 A CN 107980670A CN 201711223864 A CN201711223864 A CN 201711223864A CN 107980670 A CN107980670 A CN 107980670A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention discloses a kind of Pelteobagrus fulvidraco induced spawning method; caught by parent population with transporting, the selection for parent population of hastening parturition and combo, oxytocic drug are prepared with injecting, artificial insemination and parent population postnatal care; technical basis is provided for the yellow fish artificial propagation in the northern area of China, is conducive to the yellow development along fish artificial breeding and the protection of a yellow fish natural resources.
Description
Technical field
The present invention relates to a kind of Pelteobagrus fulvidraco induced spawning method.
Background technology
Although China's Culture of Pelteobagrus Fulvidraco has very fast development in recent years, Seedling production is always to restrict yellow fish hair
The main bottleneck of exhibition.Seed needed for Culture of Pelteobagrus Fulvidraco relies primarily on Pelteobagrus fulvidraco natural propagation and buys.Pelteobagrus fulvidraco is naturally numerous
Seed low output and unstable is grown, parent population or fry is fished in natural water area, can also further destroy the natural resources of Pelteobagrus fulvidraco:
Poor from the universal adaptability of seed bought, survival rate is low.
The content of the invention
It is proposed in the above background technology to solve it is an object of the invention to provide a kind of Pelteobagrus fulvidraco induced spawning method
Problem.
To achieve the above object, the present invention provides following technical solution：A kind of Pelteobagrus fulvidraco induced spawning method, including it is following
Step,
S1, parent population fishing and transport, parent fish pond parent population are caught using seine, and long 100m is netted in seine, nets high 2m, mesh 2cm, etting
Using polyethylene knotless netting, operation is drawn in the net carefully, action is light and slow, has stepped on bottom line, and seine speed is slow, in order to avoid injure fish body or escape
Ease, after parent population is debarked by fishing, it is spare to load parent population special plastic basket by parent population；
The parent population in adult fish culture pond is caught with cage, cage is arranged on offshore shoal, 2-4h checks that once discovery has angle to enter
Cage is drawn off immediately；
Parent population takes dry method to transport, and the ripe good parent population of selection is put into parent population special plastic basket, water is not added with and directly transports, and one
As 1h or so can be maintained not interfere with the normal physiological activity of fish body in 20 DEG C of temperature, transportation range is no more than 500m；
S2, the selection for parent population of hastening parturition and combo：A yellow fish parent fish nurturing period running check parent population gonad development situation, parent population property
The judgement of gland Developmental maturity, takes two methods:
Method one:The maturity development of ovary maturity for judging parent population gonad development is judged and touched with hand from the appearance of fish body
Good female parent's fish belly portion is expanded, and faces upward that the visible ovary profile of abdomen is obvious, and handstand has ovary flow phenomenon;Reproduction bore expansion, it is wide and justify,
Take on a red color, touch parent population belly with hand, feel soft high resilience, milter body colour is more deep yellow, and belly does not expand, gonapophysis it is long and
Point, in pink；
Method two:Judge raun gonadal maturation degree using egg apparatus digging ovum is dug, egg apparatus will be dug and be slowly inserted into gonopore, stretched into
About 2cm is deep, under rotation is several, ovum is taken out and is placed in glass dish, add 2-3 ml transparent liquids, after soaking 3min, when archiblast is semi-transparent
When bright and core is still opaque, observe under the microscope, oneself eccentric or (egg nucleus in polarized state of the core in every major part ovum grain
Positioned at egg membrane edge), illustrate that N phase egg mother cells are developed to mid-term and latter stage respectively, gonadal maturation degree is good:Most ovum grains
Core person placed in the middle, gonad development is not yet ripe, and most ovum grains are seedless as person, the own overmatureration of ovum；
The preparation method of transparent liquid:95% 5 parts of alcohol, 1 part of glacial acetic acid, the 4 parts of mixing of the saturating alcohol of turpentine oil.
The ripe good parent population of selection is weighed, is counted, the female milter combo ratio of a yellow fish parent fish is true according to fertilization method
It is fixed；
S3, condition of hastening parturition
Spawning inducing pool, spawning inducing pool are located at away from a Huang fish parent fish pond 500m, and spawning inducing pool is cement pit, area 8m2, specification 4m*2m*1m,
Water injection is convenient, is shaded using tarpaulin；Spawning inducing pool is first scrubbed clean using preceding with clear water, then the formalin with concentration 20mg/L
Solution soaking disinfection；
Hasten parturition net cage, cage size 2m*2m*1 m, do frame, the bolting silk of 60 mesh does etting, net of hastening parturition using diameter 10mm reinforcing bars
Case is arranged in pond, is fixed with timber, and net cage underwater penetration is 70cm, and it is in rods arranged in horizontal line that net cage is set in pond, net cage
Spacing 2m, sets the pond area 67m for net cage of hastening parturition2, pond mean depth 2m, husky bottom, close to the big Hai Hu that bursts, water source is big stain
Extra large lake water, for lake water through penetrating into pond, water quality is pure and fresh, pollution-free, and net cage of hastening parturition first is scrubbed using preceding with brush in clear water
Totally, then with the formalin solution of concentration 20mg/L sterilize；
Parent population temporarily supports net cage, and parent population temporarily supports the parent population cage size 2m*2m*1m that net cage is used to temporarily support the first pin ocyodinic of injection,
Using the reinforcing bar of diameter 10mm as frame, 1 cm of mesh without tubercle polyethylene mesh sheet as etting.Net cage is arranged on pond
Offshore shoal, is fixed with timber, net cage underwater penetration 50cm；
Artificial fish nest, selects palm fibre to put skin as fish nest, puts skin to new purchase palm fibre and first boil 30 min in water, then expose in the sun
Shine, spare after airing, under the conditions of natural spawning fertilization, fish nest method for arranging is as follows:Palm fibre is put into skin and is equably sewn on long 2m, width
On the bolting silk of 1m, 40 mesh of bolting silk, bolting silk makees frame using the reinforcing bar of diameter 5mm, the palm fibre being sewn on bolting silk is put skin is laid in and urge
Pond or net cage bottom of hastening parturition are produced, is compacted with stone, is used as fish nest.After fish oviposition, the palm fibre for being stained with embryonated egg is put into skin and is cut
It is hung in hatchery or net cage and hatches, during artificial insemination, first soaks brown paulownia skin, then embryonated egg is spread in uniform in palm fibre and puts skin
On, the palm fibre for being stained with embryonated egg is put skin and is hung in hatchery or net cage and is hatched, after prelarva incubation of membrane, palm fibre is put into skin in time and is taken
Go out, clean up, and sterilized with the formalin solution of concentration 20mg/L, it is spare after airing；
Water source, source quality is pure and fresh, pollution-free, and the water for injecting spawning inducing pool will be urged through 120 mesh silk cover filterings
Produce water depth 0.6-0.8m；
S4, oxytocic drug is prepared and injection, and oxytocic drug is with 0.7% fish with being used after physiological saline solution.Prepare dioxone
During DOM, medicine is placed in dry mortar and ground, then add physiological saline to be used after suspension is made, chorionic gonadotrophin swashs
Plain HCG, Leuteinizing hormone releasing hormone analogue LRH-AZ are directly dissolved in physiological saline, per 10g parent population liquid medicine injections 0.06-
0.10ml is general per tail fish injection 0.3-1.0ml.Liquid volume is calculated according to parent population gross weight, oxytocic drug is now with the current；
Injection tool takes intraperitoneal injection, injection site using 1 m of specification, the sterilized medical syringe of 2.5 blood, all parent populations
In the recess of a yellow fish pectoral fin base portion, injection, two people's compounding practices, a human hand held fish head portion and afterbody, another people inject
Liquid, " angle, when operation, are careful, stab, injection depth 3-4mm, control to prevent the hard spine of Pelteobagrus fulvidraco in 45 for syringe and fish body body axis
Good injection depth, in order to avoid injure internal organ；
Parent population takes double injection, and double injection interval time is 12-14h, the time of parent population injection for the first time usually at night,
Second of injection morning next day carries out, and double injection is carried out in the pectoral fin of not ipsilateral, in order to avoid liquid medicine spill；Parent population is noted for the first time
After penetrating, female milter, which separates, temporarily to be supported, and after the injection of the second pin, female milter is put into togerther spawning inducing pool；
S5, artificial insemination,
Equipment prepares, parent population special plastic fish basket, diddle-net, mortar, elbow operating scissors, enamel basin diameter 20cm, towel, face-cloth
Paper, palm fibre put skin, 0.7% fish physiological saline；
Adopt ovum and insemination parent population prepare, after injecting the second pin ocyodinic, parent population is put into the spawning inducing pool for completing fish nest or net cage,
Expected from parent population of hastening parturition arrival during Effect time, a parent population is checked every 2h, when finding that parent population starts oviposition, by parent population
All catch out, select extruding belly, the raun of the smooth outflow of ovum grain energy prepares to adopt ovum.Again in the ripe good male parent of combo ratio selection
Fish, is respectively put into special parent population plastic fish basket, and the male and female parent population for not adopting ovum puts back to net cage of hastening parturition, and is checked once every 2h later
Parent population, selects extruding belly, and the female parent population of the smooth outflow of ovum grain energy carries out ovum artificial collection insemination；
Artificial insemination, adopts ovum, portion's abdomen crushing grinding method semen collection, semidry method insemination, before squeezing ovum, first uses towel using crowded abdomen method
Parent population body surface moisture, when adopting ovum operation, holds fish head portion, head-up, under Caudad, gonopore is against enamel basin, with forefinger and thumb
Refer to extruding fish belly portion to squeeze into fish-egg in dry enamel basin, at the same time, ripe good male parent population is taken, with elbow operating scissors
From anus to forward position stomach wall abdominal cut, stir internal organ and expose spermary, win spermary and be put into mortar, not hinder internal organ during operation
For principle, in case bleeding, the dirts such as the blood on net spermary surface are blotted with face tissue, then spermary is shredded with scissors, fully grinds
Mill, adds fish normal saline dilution o.7%, diluted sperm is then poured into oneself rapidly and is squeezed into the enamel basin of fish-egg
It is stirred with together with ovum, is sufficiently mixed smart ovum.Then the water of filtering is added, stirs 2-3min, static 30s, then use pond
Water rinses, and washes away unnecessary spermary tissue, sperm and other dirts, finally by embryonated egg uniformly dispersing on brown paulownia skin, prepares
Hatching, whole insemination operation carry out under conditions of shady and cool；
S6, parent population postnatal care, for unscathed parent population, postpartum is directly placed into pond and continues to raise.For the parent to sustain a slight injury
Fish, embathes fish body disinfection 20min with the liquor potassic permanganate of concentration 20mg/L, pond is then placed into, for injured heavier
Parent population, except embathing fish body disinfection 20 ~ outer with the liquor potassic permanganate of concentration 20mg/L, then injects penicillin and is treated, note
It is 80,000 IU/kg to penetrate dose.
Compared with prior art, the beneficial effects of the invention are as follows：This research is the yellow fish artificial propagation in the northern area of China
Technical basis is provided, is conducive to the yellow development along fish artificial breeding and the protection of a yellow fish natural resources.
Embodiment
The technical solution in the embodiment of the present invention is clearly and completely described below, it is clear that described embodiment
Only part of the embodiment of the present invention, instead of all the embodiments.Based on the embodiments of the present invention, the common skill in this area
Art personnel all other embodiments obtained without making creative work, belong to the model that the present invention protects
Enclose.
A kind of Pelteobagrus fulvidraco induced spawning method, comprises the following steps,
S1, parent population fishing and transport, parent fish pond parent population are caught using seine, and long 100m is netted in seine, nets high 2m, mesh 2cm, etting
Using polyethylene knotless netting, operation is drawn in the net carefully, action is light and slow, has stepped on bottom line, and seine speed is slow, in order to avoid injure fish body or escape
Ease, after parent population is debarked by fishing, it is spare to load parent population special plastic basket by parent population；
The parent population in adult fish culture pond is caught with cage, cage is arranged on offshore shoal, 2-4h checks that once discovery has angle to enter
Cage is drawn off immediately；
Parent population takes dry method to transport, and the ripe good parent population of selection is put into parent population special plastic basket, water is not added with and directly transports, and one
As 1h or so can be maintained not interfere with the normal physiological activity of fish body in 20 DEG C of temperature, transportation range is no more than 500m；
S2, the selection for parent population of hastening parturition and combo：A yellow fish parent fish nurturing period running check parent population gonad development situation, parent population property
The judgement of gland Developmental maturity, takes two methods:
Method one:The maturity development of ovary maturity for judging parent population gonad development is judged and touched with hand from the appearance of fish body
Good female parent's fish belly portion is expanded, and faces upward that the visible ovary profile of abdomen is obvious, and handstand has ovary flow phenomenon;Reproduction bore expansion, it is wide and justify,
Take on a red color, touch parent population belly with hand, feel soft high resilience, milter body colour is more deep yellow, and belly does not expand, gonapophysis it is long and
Point, in pink；
Method two:Judge raun gonadal maturation degree using egg apparatus digging ovum is dug, egg apparatus will be dug and be slowly inserted into gonopore, stretched into
About 2cm is deep, under rotation is several, ovum is taken out and is placed in glass dish, add 2-3 ml transparent liquids, after soaking 3min, when archiblast is semi-transparent
When bright and core is still opaque, observe under the microscope, oneself eccentric or (egg nucleus in polarized state of the core in every major part ovum grain
Positioned at egg membrane edge), illustrate that N phase egg mother cells are developed to mid-term and latter stage respectively, gonadal maturation degree is good:Most ovum grains
Core person placed in the middle, gonad development is not yet ripe, and most ovum grains are seedless as person, the own overmatureration of ovum；
The preparation method of transparent liquid:95% 5 parts of alcohol, 1 part of glacial acetic acid, the 4 parts of mixing of the saturating alcohol of turpentine oil.
The ripe good parent population of selection is weighed, is counted, the female milter combo ratio of a yellow fish parent fish is true according to fertilization method
It is fixed；
S3, condition of hastening parturition
Spawning inducing pool, spawning inducing pool are located at away from a Huang fish parent fish pond 500m, and spawning inducing pool is cement pit, area 8m2, specification 4m*2m*1m,
Water injection is convenient, is shaded using tarpaulin；Spawning inducing pool is first scrubbed clean using preceding with clear water, then the formalin with concentration 20mg/L
Solution soaking disinfection；
Hasten parturition net cage, cage size 2m*2m*1 m, do frame, the bolting silk of 60 mesh does etting, net of hastening parturition using diameter 10mm reinforcing bars
Case is arranged in pond, is fixed with timber, and net cage underwater penetration is 70cm, and it is in rods arranged in horizontal line that net cage is set in pond, net cage
Spacing 2m, sets the pond area 67m for net cage of hastening parturition2, pond mean depth 2m, husky bottom, close to the big Hai Hu that bursts, water source is big stain
Extra large lake water, for lake water through penetrating into pond, water quality is pure and fresh, pollution-free, and net cage of hastening parturition first is scrubbed using preceding with brush in clear water
Totally, then with the formalin solution of concentration 20mg/L sterilize；
Parent population temporarily supports net cage, and parent population temporarily supports the parent population cage size 2m*2m*1m that net cage is used to temporarily support the first pin ocyodinic of injection,
Using the reinforcing bar of diameter 10mm as frame, 1 cm of mesh without tubercle polyethylene mesh sheet as etting.Net cage is arranged on pond
Offshore shoal, is fixed with timber, net cage underwater penetration 50cm；
Artificial fish nest, selects palm fibre to put skin as fish nest, puts skin to new purchase palm fibre and first boil 30 min in water, then expose in the sun
Shine, spare after airing, under the conditions of natural spawning fertilization, fish nest method for arranging is as follows:Palm fibre is put into skin and is equably sewn on long 2m, width
On the bolting silk of 1m, 40 mesh of bolting silk, bolting silk makees frame using the reinforcing bar of diameter 5mm, the palm fibre being sewn on bolting silk is put skin is laid in and urge
Pond or net cage bottom of hastening parturition are produced, is compacted with stone, is used as fish nest.After fish oviposition, the palm fibre for being stained with embryonated egg is put into skin and is cut
It is hung in hatchery or net cage and hatches, during artificial insemination, first soaks brown paulownia skin, then embryonated egg is spread in uniform in palm fibre and puts skin
On, the palm fibre for being stained with embryonated egg is put skin and is hung in hatchery or net cage and is hatched, after prelarva incubation of membrane, palm fibre is put into skin in time and is taken
Go out, clean up, and sterilized with the formalin solution of concentration 20mg/L, it is spare after airing；
Water source, source quality is pure and fresh, pollution-free, and the water for injecting spawning inducing pool will be urged through 120 mesh silk cover filterings
Produce water depth 0.6-0.8m；
S4, oxytocic drug is prepared and injection, and oxytocic drug is with 0.7% fish with being used after physiological saline solution.Prepare dioxone
During DOM, medicine is placed in dry mortar and ground, then add physiological saline to be used after suspension is made, chorionic gonadotrophin swashs
Plain HCG, Leuteinizing hormone releasing hormone analogue LRH-AZ are directly dissolved in physiological saline, per 10g parent population liquid medicine injections 0.06-
0.10ml is general per tail fish injection 0.3-1.0ml.Liquid volume is calculated according to parent population gross weight, oxytocic drug is now with the current；
Injection tool takes intraperitoneal injection, injection site using 1 m of specification, the sterilized medical syringe of 2.5 blood, all parent populations
In the recess of a yellow fish pectoral fin base portion, injection, two people's compounding practices, a human hand held fish head portion and afterbody, another people inject
Liquid, " angle, when operation, are careful, stab, injection depth 3-4mm, control to prevent the hard spine of Pelteobagrus fulvidraco in 45 for syringe and fish body body axis
Good injection depth, in order to avoid injure internal organ；
Parent population takes double injection, and double injection interval time is 12-14h, the time of parent population injection for the first time usually at night,
Second of injection morning next day carries out, and double injection is carried out in the pectoral fin of not ipsilateral, in order to avoid liquid medicine spill；Parent population is noted for the first time
After penetrating, female milter, which separates, temporarily to be supported, and after the injection of the second pin, female milter is put into togerther spawning inducing pool；
S5, artificial insemination,
Equipment prepares, parent population special plastic fish basket, diddle-net, mortar, elbow operating scissors, enamel basin diameter 20cm, towel, face-cloth
Paper, palm fibre put skin, 0.7% fish physiological saline；
Adopt ovum and insemination parent population prepare, after injecting the second pin ocyodinic, parent population is put into the spawning inducing pool for completing fish nest or net cage,
Expected from parent population of hastening parturition arrival during Effect time, a parent population is checked every 2h, when finding that parent population starts oviposition, by parent population
All catch out, select extruding belly, the raun of the smooth outflow of ovum grain energy prepares to adopt ovum.Again in the ripe good male parent of combo ratio selection
Fish, is respectively put into special parent population plastic fish basket, and the male and female parent population for not adopting ovum puts back to net cage of hastening parturition, and is checked once every 2h later
Parent population, selects extruding belly, and the female parent population of the smooth outflow of ovum grain energy carries out ovum artificial collection insemination；
Artificial insemination, adopts ovum, portion's abdomen crushing grinding method semen collection, semidry method insemination, before squeezing ovum, first uses towel using crowded abdomen method
Parent population body surface moisture, when adopting ovum operation, holds fish head portion, head-up, under Caudad, gonopore is against enamel basin, with forefinger and thumb
Refer to extruding fish belly portion to squeeze into fish-egg in dry enamel basin, at the same time, ripe good male parent population is taken, with elbow operating scissors
From anus to forward position stomach wall abdominal cut, stir internal organ and expose spermary, win spermary and be put into mortar, not hinder internal organ during operation
For principle, in case bleeding, the dirts such as the blood on net spermary surface are blotted with face tissue, then spermary is shredded with scissors, fully grinds
Mill, adds fish normal saline dilution o.7%, diluted sperm is then poured into oneself rapidly and is squeezed into the enamel basin of fish-egg
It is stirred with together with ovum, is sufficiently mixed smart ovum.Then the water of filtering is added, stirs 2-3min, static 30s, then use pond
Water rinses, and washes away unnecessary spermary tissue, sperm and other dirts, finally by embryonated egg uniformly dispersing on brown paulownia skin, prepares
Hatching, whole insemination operation carry out under conditions of shady and cool；
S6, parent population postnatal care, for unscathed parent population, postpartum is directly placed into pond and continues to raise.For the parent to sustain a slight injury
Fish, embathes fish body disinfection 20min with the liquor potassic permanganate of concentration 20mg/L, pond is then placed into, for injured heavier
Parent population, except embathing fish body disinfection 20 ~ outer with the liquor potassic permanganate of concentration 20mg/L, then injects penicillin and is treated, note
It is 80,000 IU/kg to penetrate dose.
Although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
Understanding without departing from the principles and spirit of the present invention can carry out these embodiments a variety of changes, modification, replace
And modification, the scope of the present invention is defined by the appended.
Claims (1)
1. a kind of Pelteobagrus fulvidraco induced spawning method, it is characterized in that：Comprise the following steps,
S1, parent population fishing and transport, parent fish pond parent population are caught using seine, and long 100m is netted in seine, nets high 2m, mesh 2cm, etting
Using polyethylene knotless netting, operation is drawn in the net carefully, action is light and slow, has stepped on bottom line, and seine speed is slow, in order to avoid injure fish body or escape
Ease, after parent population is debarked by fishing, it is spare to load parent population special plastic basket by parent population；
The parent population in adult fish culture pond is caught with cage, cage is arranged on offshore shoal, 2-4h checks that once discovery has angle to enter
Cage is drawn off immediately；
Parent population takes dry method to transport, and the ripe good parent population of selection is put into parent population special plastic basket, water is not added with and directly transports, and one
As 1h or so can be maintained not interfere with the normal physiological activity of fish body in 20 DEG C of temperature, transportation range is no more than 500m；
S2, the selection for parent population of hastening parturition and combo：A yellow fish parent fish nurturing period running check parent population gonad development situation, parent population property
The judgement of gland Developmental maturity, takes two methods:
Method one:The maturity development of ovary maturity for judging parent population gonad development is judged and touched with hand from the appearance of fish body
Good female parent's fish belly portion is expanded, and faces upward that the visible ovary profile of abdomen is obvious, and handstand has ovary flow phenomenon;Reproduction bore expansion, it is wide and justify,
Take on a red color, touch parent population belly with hand, feel soft high resilience, milter body colour is more deep yellow, and belly does not expand, gonapophysis it is long and
Point, in pink；
Method two:Judge raun gonadal maturation degree using egg apparatus digging ovum is dug, egg apparatus will be dug and be slowly inserted into gonopore, stretched into
About 2cm is deep, under rotation is several, ovum is taken out and is placed in glass dish, add 2-3 ml transparent liquids, after soaking 3min, when archiblast is semi-transparent
When bright and core is still opaque, observe under the microscope, oneself eccentric or (egg nucleus in polarized state of the core in every major part ovum grain
Positioned at egg membrane edge), illustrate that N phase egg mother cells are developed to mid-term and latter stage respectively, gonadal maturation degree is good:Most ovum grains
Core person placed in the middle, gonad development is not yet ripe, and most ovum grains are seedless as person, the own overmatureration of ovum；
The preparation method of transparent liquid:95% 5 parts of alcohol, 1 part of glacial acetic acid, the 4 parts of mixing of the saturating alcohol of turpentine oil；By the ripe good of selection
Parent population weighs, counts, and the yellow female milter combo ratio of a fish parent fish is determined according to fertilization method；
S3, condition of hastening parturition
Spawning inducing pool, spawning inducing pool are located at away from a Huang fish parent fish pond 500m, and spawning inducing pool is cement pit, area 8m2, specification 4m*2m*1m, note
Draining is convenient, is shaded using tarpaulin；Spawning inducing pool is first scrubbed clean using preceding with clear water, then molten with the formalin of concentration 20mg/L
Liquid soaking disinfection；
Hasten parturition net cage, cage size 2m*2m*1 m, do frame, the bolting silk of 60 mesh does etting, net of hastening parturition using diameter 10mm reinforcing bars
Case is arranged in pond, is fixed with timber, and net cage underwater penetration is 70cm, and it is in rods arranged in horizontal line that net cage is set in pond, net cage
Spacing 2m, sets the pond area 67m for net cage of hastening parturition2, pond mean depth 2m, husky bottom, close to the big Hai Hu that bursts, water source is big stain
Extra large lake water, for lake water through penetrating into pond, water quality is pure and fresh, pollution-free, and net cage of hastening parturition first is scrubbed using preceding with brush in clear water
Totally, then with the formalin solution of concentration 20mg/L sterilize；
Parent population temporarily supports net cage, and parent population temporarily supports the parent population cage size 2m*2m*1m that net cage is used to temporarily support the first pin ocyodinic of injection,
Using the reinforcing bar of diameter 10mm as frame, 1 cm of mesh without tubercle polyethylene mesh sheet as etting；Net cage is arranged on pond
Offshore shoal, is fixed with timber, net cage underwater penetration 50cm；
Artificial fish nest, selects palm fibre to put skin as fish nest, puts skin to new purchase palm fibre and first boil 30 min in water, then expose in the sun
Shine, spare after airing, under the conditions of natural spawning fertilization, fish nest method for arranging is as follows:Palm fibre is put into skin and is equably sewn on long 2m, width
On the bolting silk of 1m, 40 mesh of bolting silk, bolting silk makees frame using the reinforcing bar of diameter 5mm, the palm fibre being sewn on bolting silk is put skin is laid in and urge
Pond or net cage bottom of hastening parturition are produced, is compacted with stone, is used as fish nest；After fish oviposition, the palm fibre for being stained with embryonated egg is put into skin and is cut
It is hung in hatchery or net cage and hatches, during artificial insemination, first soaks brown paulownia skin, then embryonated egg is spread in uniform in palm fibre and puts skin
On, the palm fibre for being stained with embryonated egg is put skin and is hung in hatchery or net cage and is hatched, after prelarva incubation of membrane, palm fibre is put into skin in time and is taken
Go out, clean up, and sterilized with the formalin solution of concentration 20mg/L, it is spare after airing；
Water source, source quality is pure and fresh, pollution-free, and the water for injecting spawning inducing pool will be urged through 120 mesh silk cover filterings
Produce water depth 0.6-0.8m；
S4, oxytocic drug is prepared and injection, and oxytocic drug is with 0.7% fish with being used after physiological saline solution；Prepare dioxone
During DOM, medicine is placed in dry mortar and ground, then add physiological saline to be used after suspension is made, chorionic gonadotrophin swashs
Plain HCG, Leuteinizing hormone releasing hormone analogue LRH-AZ are directly dissolved in physiological saline, per 10g parent population liquid medicine injections 0.06-
0.10ml is general per tail fish injection 0.3-1.0ml；Liquid volume is calculated according to parent population gross weight, oxytocic drug is now with the current；
Injection tool takes intraperitoneal injection, injection site using 1 m of specification, the sterilized medical syringe of 2.5 blood, all parent populations
In the recess of a yellow fish pectoral fin base portion, injection, two people's compounding practices, a human hand held fish head portion and afterbody, another people inject
Liquid, " angle, when operation, are careful, stab, injection depth 3-4mm, control to prevent the hard spine of Pelteobagrus fulvidraco in 45 for syringe and fish body body axis
Good injection depth, in order to avoid injure internal organ；
Parent population takes double injection, and double injection interval time is 12-14h, the time of parent population injection for the first time usually at night,
Second of injection morning next day carries out, and double injection is carried out in the pectoral fin of not ipsilateral, in order to avoid liquid medicine spill；Parent population is noted for the first time
After penetrating, female milter, which separates, temporarily to be supported, and after the injection of the second pin, female milter is put into togerther spawning inducing pool；
S5, artificial insemination,
Equipment prepares, parent population special plastic fish basket, diddle-net, mortar, elbow operating scissors, enamel basin diameter 20cm, towel, face-cloth
Paper, palm fibre put skin, 0.7% fish physiological saline；
Adopt ovum and insemination parent population prepare, after injecting the second pin ocyodinic, parent population is put into the spawning inducing pool for completing fish nest or net cage,
Expected from parent population of hastening parturition arrival during Effect time, a parent population is checked every 2h, when finding that parent population starts oviposition, by parent population
All catch out, select extruding belly, the raun of the smooth outflow of ovum grain energy prepares to adopt ovum；Again in the ripe good male parent of combo ratio selection
Fish, is respectively put into special parent population plastic fish basket, and the male and female parent population for not adopting ovum puts back to net cage of hastening parturition, and is checked once every 2h later
Parent population, selects extruding belly, and the female parent population of the smooth outflow of ovum grain energy carries out ovum artificial collection insemination；
Artificial insemination, adopts ovum, portion's abdomen crushing grinding method semen collection, semidry method insemination, before squeezing ovum, first uses towel using crowded abdomen method
Parent population body surface moisture, when adopting ovum operation, holds fish head portion, head-up, under Caudad, gonopore is against enamel basin, with forefinger and thumb
Refer to extruding fish belly portion to squeeze into fish-egg in dry enamel basin, at the same time, ripe good male parent population is taken, with elbow operating scissors
From anus to forward position stomach wall abdominal cut, stir internal organ and expose spermary, win spermary and be put into mortar, not hinder internal organ during operation
For principle, in case bleeding, the dirts such as the blood on net spermary surface are blotted with face tissue, then spermary is shredded with scissors, fully grinds
Mill, adds fish normal saline dilution o.7%, diluted sperm is then poured into oneself rapidly and is squeezed into the enamel basin of fish-egg
It is stirred with together with ovum, is sufficiently mixed smart ovum；Then the water of filtering is added, stirs 2-3min, static 30s, then use pond
Water rinses, and washes away unnecessary spermary tissue, sperm and other dirts, finally by embryonated egg uniformly dispersing on brown paulownia skin, prepares
Hatching, whole insemination operation carry out under conditions of shady and cool；
S6, parent population postnatal care, for unscathed parent population, postpartum is directly placed into pond and continues to raise；For the parent to sustain a slight injury
Fish, embathes fish body disinfection 20min with the liquor potassic permanganate of concentration 20mg/L, pond is then placed into, for injured heavier
Parent population, except embathing fish body disinfection 20 ~ outer with the liquor potassic permanganate of concentration 20mg/L, then injects penicillin and is treated, note
It is 80,000 IU/kg to penetrate dose.
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