CN106508740A - Yellow-head catfish meat artificial reproduction method - Google Patents
Yellow-head catfish meat artificial reproduction method Download PDFInfo
- Publication number
- CN106508740A CN106508740A CN201610923261.XA CN201610923261A CN106508740A CN 106508740 A CN106508740 A CN 106508740A CN 201610923261 A CN201610923261 A CN 201610923261A CN 106508740 A CN106508740 A CN 106508740A
- Authority
- CN
- China
- Prior art keywords
- fish
- injection
- artificial
- parent fish
- ovum
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims abstract description 19
- 235000013372 meat Nutrition 0.000 title abstract description 4
- 241000367571 Mohoua ochrocephala Species 0.000 title abstract 3
- 241001233037 catfish Species 0.000 title abstract 3
- 241000251468 Actinopterygii Species 0.000 claims abstract description 72
- 238000002347 injection Methods 0.000 claims abstract description 33
- 239000007924 injection Substances 0.000 claims abstract description 33
- 239000003814 drug Substances 0.000 claims abstract description 11
- 230000004720 fertilization Effects 0.000 claims abstract description 7
- 238000002360 preparation method Methods 0.000 claims abstract description 4
- 102000002322 Egg Proteins Human genes 0.000 claims description 20
- 108010000912 Egg Proteins Proteins 0.000 claims description 20
- 210000004681 ovum Anatomy 0.000 claims description 20
- 230000003187 abdominal effect Effects 0.000 claims description 12
- 230000001939 inductive effect Effects 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 9
- 210000000582 semen Anatomy 0.000 claims description 9
- 241001471302 Tachysurus Species 0.000 claims description 8
- 239000004570 mortar (masonry) Substances 0.000 claims description 7
- 229930182555 Penicillin Natural products 0.000 claims description 6
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 claims description 6
- 208000027418 Wounds and injury Diseases 0.000 claims description 6
- 210000001015 abdomen Anatomy 0.000 claims description 6
- 230000000845 anti-microbial effect Effects 0.000 claims description 6
- 239000004568 cement Substances 0.000 claims description 6
- 229940079593 drug Drugs 0.000 claims description 6
- 230000002196 ecbolic effect Effects 0.000 claims description 6
- 230000000694 effects Effects 0.000 claims description 6
- 239000000835 fiber Substances 0.000 claims description 6
- 230000009027 insemination Effects 0.000 claims description 6
- 210000001672 ovary Anatomy 0.000 claims description 6
- 229940049954 penicillin Drugs 0.000 claims description 6
- 239000000243 solution Substances 0.000 claims description 5
- 210000004247 hand Anatomy 0.000 claims description 4
- 239000007788 liquid Substances 0.000 claims description 4
- 230000008569 process Effects 0.000 claims description 4
- 241001071795 Gentiana Species 0.000 claims description 3
- 235000003140 Panax quinquefolius Nutrition 0.000 claims description 3
- 240000005373 Panax quinquefolius Species 0.000 claims description 3
- 241000124033 Salix Species 0.000 claims description 3
- 206010052428 Wound Diseases 0.000 claims description 3
- 206010048038 Wound infection Diseases 0.000 claims description 3
- 239000011449 brick Substances 0.000 claims description 3
- 230000006378 damage Effects 0.000 claims description 3
- 235000013601 eggs Nutrition 0.000 claims description 3
- 208000014674 injury Diseases 0.000 claims description 3
- 210000003097 mucus Anatomy 0.000 claims description 3
- 230000032696 parturition Effects 0.000 claims description 3
- 210000000006 pectoral fin Anatomy 0.000 claims description 3
- 239000012286 potassium permanganate Substances 0.000 claims description 3
- 210000004872 soft tissue Anatomy 0.000 claims description 3
- 239000004575 stone Substances 0.000 claims description 3
- 230000032258 transport Effects 0.000 claims description 3
- 239000004599 antimicrobial Substances 0.000 claims 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims 1
- 239000010931 gold Substances 0.000 claims 1
- 229910052737 gold Inorganic materials 0.000 claims 1
- 239000002574 poison Substances 0.000 claims 1
- 231100000614 poison Toxicity 0.000 claims 1
- 230000004083 survival effect Effects 0.000 abstract description 3
- 230000008901 benefit Effects 0.000 abstract description 2
- 230000004075 alteration Effects 0.000 abstract 1
- 241000376029 Tachysurus fulvidraco Species 0.000 description 3
- 241000252229 Carassius auratus Species 0.000 description 2
- 230000002159 abnormal effect Effects 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000035807 sensation Effects 0.000 description 2
- 241000252073 Anguilliformes Species 0.000 description 1
- 241000158704 Monopterus albus Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention provides a yellow-head catfish meat artificial reproduction method. The method comprises the steps of 1, selection and matching of parent fishes; preparation of an induced spawning pond and an artificial fish spawning nest; 3, injection of induced spawning medicine; 4, artificial pawning and fertilization; 5, postpartum care of the parent fish. The yellow-head catfish meat artificial reproduction method has the advantages of being high in reproduction success rate, efficiency and fry survival rate and low in aberration rate.
Description
Technical field
A kind of the present invention relates to Pelteobagrus fulvidraco breeding field, more particularly to artificial propagation method of pelteobagrus fulvdraco.
Background technology
Pelteobagrus fulvidraco is under the jurisdiction of to Xing Mu Chinese yellow eels section, Monopterus albus (Zuiew) fish category, is a kind of important economic fish in Chinese rivers and lakes,
The characteristics of good characteristic having due to a yellow fish and delicious meat, its artificial cultivation is widely paid attention to.
During artificial cultivation, the artificial propagation of Pelteobagrus fulvidraco is most important link, relative to natural propagation, can be overcome
The efficiency of natural propagation process is low, survival rate of fish fry is low, the shortcoming that abnormal rate is high.
The content of the invention
The present invention provides a kind of artificial propagation method of pelteobagrus fulvdraco, comprises the step of:
1) selection of parent fish and combo:
Observation and the method for touching is taken, selection standard is:Ripe good dam's fish belly portion expands, and faces upward abdomen visible significantly
Ovary profile, handstand have an ovary flow phenomenon, reproduction bore expansion, wide and justify, and take on a red color, and handss touch abdominal part soft and high resilience
Sensation;Ripe good milter body colour is more deep yellow, and abdominal part does not expand, gonapophysis length and it is sharp, in pink;
The proportioning of female, male parent fish of artificial insemination is adopted for 2:1-8:1;
2) preparation of spawning inducing pool and artificial fish nest:
Artificial insemination adopts cement pit or small-sized net cage as spawning inducing pool, cement pool area 4-10m2, depth of water 0.6-0.8m;It is little
Net cage area 0.48-9m2, high lm adds a cover cover from sun above spawning inducing pool;
The tiling in spawning inducing pool bottom arranges fish nest, the one kind in putting skin, mesh sheet with willow Rhizoma Imperatae, alfa, palm fibre, uses stone
Or fragment of brick compacting fish nest, on fish nest, things concealed is set;
3) injection of oxytocic drug:
Female parent fish injection dosage is HCG200IU/ tails, LRH-A21 μ g/ tails, DOM1mg/ tails, the injection dosage of male parent fish
It is the half of the injection dosage of female Carassius auratuses;
Injection site is injected into pin depth 0.3-l.0cm in pectoral fin base portion or dorsal fin base portion soft tissue, and female parent fish takes two
Secondary injection, interval time of double injection is 12-24h, and male parent fish adopts a shot;19.5 DEG C -22 of injection time control controlling the water circulation temperature
DEG C, Effect time is 35-30h;
4) artificial spawning fertilization:
After injection oxytocic drug, after Effect time, parent fish is made regular check on once every 1h, once find that indivedual rauns produce
Ovum, carries out semen collection immediately and adopts ovum;
Semen collection is the raun for selecting the smooth outflow of light pressure abdominal part ovum grain energy, first cuts off milter abdominal part, and picking spermary is put into grinds
Shred in alms bowl, grind pressure, add 0.7% normal saline or Ge Linshi liquid, or be initially charged in mortar 0.7% normal saline or
Ringer's solution, the spermary of a few tail fishes is bound up with gauze and grinds pressure in mortar;
Semen collection carries out adopting ovum simultaneously, and fish roe is clamp-oned in dry container, when squeezing fish roe, will dry raun body surface and operation
Person's moisture on hand;
Adopt after ovum terminates, the sperm of collection is poured in Sheng ovum container, is continuously agitated, added clear water, make smart ovum abundant
Mixing, completes fertilization process;
5) postpartum care of parent fish:
Parent fish is laid eggs, fishes for, transports and is hastened parturition through row's essence after injection, it may appear that body surface is abraded, stabbed, fin ray tears,
Epidermal mucus disappear, dead situation, and slight wound can carry out medicine with potassium permanganate solution, gentian voiet, saline solution and embathe fish
Body is sterilized, in case wound infection;The parent fish in puerperal of major injuries, in addition to body surface is sterilized, should also inject antimicrobial DP finish.
Further, the things concealed arranged on described fish nest is that pasture and water or palm fibre put skin.
Further, described antimicrobial DP finish is penicillin, and the injection dosage of the penicillin is 5-8IU/kg.
The invention has the beneficial effects as follows:A kind of artificial propagation method of pelteobagrus fulvdraco of the present invention, with breeding success rate
Height, efficiency high, survival rate of fish fry are high, the advantage that abnormal rate is low.
Specific embodiment
It is described in detail below with reference to embodiments of the invention.
A kind of artificial propagation method of pelteobagrus fulvdraco, comprises the step of:
1) selection of parent fish and combo:
Observation and the method for touching is taken, selection standard is:Ripe good dam's fish belly portion expands, and faces upward abdomen visible significantly
Ovary profile, handstand have an ovary flow phenomenon, reproduction bore expansion, wide and justify, and take on a red color, and handss touch abdominal part soft and high resilience
Sensation;Ripe good milter body colour is more deep yellow, and abdominal part does not expand, gonapophysis length and it is sharp, in pink;
The proportioning of female, male parent fish of artificial insemination is adopted for 2:1-8:1;
2) preparation of spawning inducing pool and artificial fish nest:
Artificial insemination adopts cement pit or small-sized net cage as spawning inducing pool, cement pool area 4-10m2, depth of water 0.6-0.8m;It is little
Net cage area 0.48-9m2, high lm adds a cover cover from sun above spawning inducing pool;
The tiling in spawning inducing pool bottom arranges fish nest, the one kind in putting skin, mesh sheet with willow Rhizoma Imperatae, alfa, palm fibre, uses stone
Or fragment of brick compacting fish nest, on fish nest, things concealed is set;
3) injection of oxytocic drug:
Female parent fish injection dosage is HCG200IU/ tails, LRH-A21 μ g/ tails, DOM1mg/ tails, the injection dosage of male parent fish
It is the half of the injection dosage of female Carassius auratuses;
Injection site is injected into pin depth 0.3-l.0cm in pectoral fin base portion or dorsal fin base portion soft tissue, and female parent fish takes two
Secondary injection, interval time of double injection is 12-24h, and male parent fish adopts a shot;19.5 DEG C -22 of injection time control controlling the water circulation temperature
DEG C, Effect time is 35-30h;
4) artificial spawning fertilization:
After injection oxytocic drug, after Effect time, parent fish is made regular check on once every 1h, once find that indivedual rauns produce
Ovum, carries out semen collection immediately and adopts ovum;
Semen collection is the raun for selecting the smooth outflow of light pressure abdominal part ovum grain energy, first cuts off milter abdominal part, and picking spermary is put into grinds
Shred in alms bowl, grind pressure, add 0.7% normal saline or Ge Linshi liquid, or be initially charged in mortar 0.7% normal saline or
Ringer's solution, the spermary of a few tail fishes is bound up with gauze and grinds pressure in mortar;
Semen collection carries out adopting ovum simultaneously, and fish roe is clamp-oned in dry container, when squeezing fish roe, will dry raun body surface and operation
Person's moisture on hand;
Adopt after ovum terminates, the sperm of collection is poured in Sheng ovum container, is continuously agitated, added clear water, make smart ovum abundant
Mixing, completes fertilization process;
5) postpartum care of parent fish:
Parent fish is laid eggs, fishes for, transports and is hastened parturition through row's essence after injection, it may appear that body surface is abraded, stabbed, fin ray tears,
Epidermal mucus disappear, dead situation, and slight wound can carry out medicine with potassium permanganate solution, gentian voiet, saline solution and embathe fish
Body is sterilized, in case wound infection;The parent fish in puerperal of major injuries, in addition to body surface is sterilized, should also inject antimicrobial DP finish.
Further, the things concealed arranged on described fish nest is that pasture and water or palm fibre put skin.
Further, described antimicrobial DP finish is penicillin, and the injection dosage of the penicillin is 5-8IU/kg.
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (3)
1. a kind of artificial propagation method of pelteobagrus fulvdraco, it is characterised in that comprise the step of:
1) selection of parent fish and combo:
Observation and the method for touching is taken, selection standard is:Ripe good dam's fish belly portion expands, and faces upward the visible obvious ovary of abdomen
Profile, handstand have an ovary flow phenomenon, reproduction bore expansion, wide and justify, and take on a red color, and handss touch abdominal part soft and whippy sense
Feel;Ripe good milter body colour is more deep yellow, and abdominal part does not expand, gonapophysis length and it is sharp, in pink;
The proportioning of female, male parent fish of artificial insemination is adopted for 2:1-8:1;
2) preparation of spawning inducing pool and artificial fish nest:
Artificial insemination adopts cement pit or small-sized net cage as spawning inducing pool, cement pool area 4-10m2, depth of water 0.6-0.8m;Small-sized net cage
Area 0.48-9m2, high lm adds a cover cover from sun above spawning inducing pool;
The tiling in spawning inducing pool bottom arranges fish nest, and the one kind in putting skin, mesh sheet with willow Rhizoma Imperatae, alfa, palm fibre, with stone or brick
Block is compacted fish nest, arranges things concealed on fish nest;
3) injection of oxytocic drug:
Female parent fish injection dosage is HCG200IU/ tails, LRH-A21 μ g/ tails, DOM1mg/ tails, the injection dosage of male parent fish is female gold
The half of the injection dosage of fish;
Injection site is injected into pin depth 0.3-l.0cm in pectoral fin base portion or dorsal fin base portion soft tissue, and female parent fish is taken and notes twice
Penetrate, interval time of double injection is 12-24h, male parent fish adopts a shot;19.5 DEG C -22 DEG C of injection time control controlling the water circulation temperature, effect
It is 35-30h between seasonable;
4) artificial spawning fertilization:
After injection oxytocic drug, after Effect time, parent fish is made regular check on once every 1h, once indivedual raun spawnings are found,
Semen collection is carried out immediately and adopts ovum;
Semen collection is the raun for selecting the smooth outflow of light pressure abdominal part ovum grain energy, first cuts off milter abdominal part, and spermary is put in mortar picking
Shred, grind pressure, add 0.7% normal saline or Ge Linshi liquid, or be initially charged 0.7% normal saline or woods lattice in mortar
Family name's liquid, the spermary of a few tail fishes is bound up with gauze and grinds pressure in mortar;
Semen collection carries out adopting ovum simultaneously, and fish roe is clamp-oned in dry container, when squeezing fish roe, will dry raun body surface and operator's handss
On moisture;
Adopt after ovum terminates, the sperm of collection poured in Sheng ovum container, is continuously agitated, added clear water, be sufficiently mixed smart ovum,
Complete fertilization process;
5) postpartum care of parent fish:
Parent fish is laid eggs, fishes for, transports and is hastened parturition through row's essence after injection, it may appear that body surface is abraded, stabbed, fin ray tears, body surface
Mucus disappears, dead situation, and slight wound can carry out medicine with potassium permanganate solution, gentian voiet, saline solution embathes fish body and disappear
Poison, in case wound infection;The parent fish in puerperal of major injuries, in addition to body surface is sterilized, should also inject antimicrobial DP finish.
2. a kind of artificial propagation method of pelteobagrus fulvdraco according to claim 1, it is characterised in that arrange on described fish nest
Things concealed is that pasture and water or palm fibre put skin.
3. a kind of artificial propagation method of pelteobagrus fulvdraco according to claim 1 and 2, it is characterised in that described anti-microbial type medicine
Thing is penicillin, and the injection dosage of the penicillin is 5-8IU/kg.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201610923261.XA CN106508740A (en) | 2016-10-29 | 2016-10-29 | Yellow-head catfish meat artificial reproduction method |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201610923261.XA CN106508740A (en) | 2016-10-29 | 2016-10-29 | Yellow-head catfish meat artificial reproduction method |
Publications (1)
Publication Number | Publication Date |
---|---|
CN106508740A true CN106508740A (en) | 2017-03-22 |
Family
ID=58291814
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201610923261.XA Pending CN106508740A (en) | 2016-10-29 | 2016-10-29 | Yellow-head catfish meat artificial reproduction method |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN106508740A (en) |
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107173265A (en) * | 2017-04-28 | 2017-09-19 | 武汉百瑞生物技术有限公司 | A kind of cultural method of female Pelteobagrus fulvidraco |
CN107683798A (en) * | 2017-09-27 | 2018-02-13 | 清远职业技术学院 | A kind of Pelteobagrus fulvidraco artificial breeding and the method for raising |
CN107912340A (en) * | 2017-11-30 | 2018-04-17 | 宁夏润宝禾农业科技有限公司 | A kind of mating system of Yellow River catfish kind |
CN107980670A (en) * | 2017-11-29 | 2018-05-04 | 安徽金阳光农业服务有限公司 | A kind of Pelteobagrus fulvidraco induced spawning method |
-
2016
- 2016-10-29 CN CN201610923261.XA patent/CN106508740A/en active Pending
Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107173265A (en) * | 2017-04-28 | 2017-09-19 | 武汉百瑞生物技术有限公司 | A kind of cultural method of female Pelteobagrus fulvidraco |
CN107683798A (en) * | 2017-09-27 | 2018-02-13 | 清远职业技术学院 | A kind of Pelteobagrus fulvidraco artificial breeding and the method for raising |
CN107980670A (en) * | 2017-11-29 | 2018-05-04 | 安徽金阳光农业服务有限公司 | A kind of Pelteobagrus fulvidraco induced spawning method |
CN107912340A (en) * | 2017-11-30 | 2018-04-17 | 宁夏润宝禾农业科技有限公司 | A kind of mating system of Yellow River catfish kind |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN106508740A (en) | Yellow-head catfish meat artificial reproduction method | |
CN104255614B (en) | A kind of schizothoracin hard Juniperus formosana Pan naked carp artificial fecundation method | |
CN101066046B (en) | Curled-lip mandarin fish and spot and spotted mandarin fish hybridizing propagation process | |
CN106900608A (en) | A kind of breeding method of big squama Barb fishes | |
CN103461251A (en) | Method for artificially propagating triplophysa siluroides | |
CN103960184B (en) | A kind of loach industrial artificial breeding improves the seedling-cultivating method of survival rate | |
CN102007874A (en) | Artificial propagation method of pelteobagrus fulvdraco | |
CN104041457B (en) | A kind of Qinling Mountains fine-scaled graphite artificial fecundation method | |
CN104642212A (en) | Artificial breeding method for onychostoma simus | |
CN103222436B (en) | Artificial propagation method of esox reichertii | |
CN105360028B (en) | The artificial fecundation method of east Triplophysa | |
CN104969887A (en) | Fishpond fish farming method | |
CN101518217A (en) | Artificial induced spawning method of glyptosternum maculatum regan | |
CN104705225A (en) | Artificial breeding method for silurus meridionalis | |
CN101461341A (en) | Induced spawning method of Brachymystax lenok | |
CN102524128A (en) | Artificial reproduction method for Pseudobagras ussuriensis | |
CN105850807A (en) | Artificial propagation method of Phoxinus lagowskii Dybowskii | |
CN103651220A (en) | Barbodes fuxianensis artificial propagation technology | |
CN104430085A (en) | Artificial reproduction method for Triplophysa yarkandensis | |
CN105248336A (en) | Artificial breeding method for glyptosternon maculatum | |
CN104145869B (en) | Artificial breeding method for acipenser dabryanus | |
Falahatkar et al. | Spawning induction in Kutum, Rutilus frisii kutum (Kamensky), with different hormones: Analysis of hormone profiles and induced spawning success | |
CN104542464A (en) | Artificial culturing method for batrachuperus tibetanus | |
CN106879515A (en) | A kind of viscidity-removing method of body graining embryonated egg high | |
CN104430169B (en) | A kind of method promoting On The Gonadal Development of Megalobatrachus Davidianus to improve breeding three rates |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20170322 |
|
WD01 | Invention patent application deemed withdrawn after publication |