CN104145869B - Artificial breeding method for acipenser dabryanus - Google Patents
Artificial breeding method for acipenser dabryanus Download PDFInfo
- Publication number
- CN104145869B CN104145869B CN201410428801.8A CN201410428801A CN104145869B CN 104145869 B CN104145869 B CN 104145869B CN 201410428801 A CN201410428801 A CN 201410428801A CN 104145869 B CN104145869 B CN 104145869B
- Authority
- CN
- China
- Prior art keywords
- acipenser dabryanus
- injection
- acipenser
- dabryanus
- artificial breeding
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention relates to the technical field of aquaculture, in particular to an artificial breeding method for acipenser dabryanus. Oxytocin is injected into female acipenser dabryanus two times, wherein the interval between two times of injection is 12 hours, and the injection amount of the first time accounts for 1/3 to 1/2 of the total dose. The one-time injection method is adopted for male acipenser dabryanus, wherein injection of the male acipenser dabryanus is synchronous with the first time of injection of the female acipenser dabryanus, and the dose of the male acipenser dabryanus is half that of the female acipenser dabryanus. Fertilization is conducted according to a half-dry method, water, eggs and seminal fluid are mixed according to the proportion of 75:50:1, and the mixture is stirred for 2 minutes to 3 minutes. A complete acipenser dabryanus artificial breeding technique process is provided, and technical support is provided for large-scale artificial breeding; due to the adoption of the method, the hatchability of acipenser dabryanus can reach 85% or higher at most, and restrict factors in the acipenser dabryanus artificial breeding process are eliminated.
Description
Technical field
The present invention relates to technical field of aquaculture, a kind of specifically artificial fecundation method of acipenser dabryanus.
Background technology
Acipenser dabryanus is China Yangtze river basin distinctive flagship species and Important Economic Fish, and it is bred and wild stockss amplification
Concern China's characteristic aquatic products industry development, the healthy and important peculiar Fish Germplasm Resources safety of aquatic ecosystem.However, due to this stream
Domain aquatic ecosystem be subject to mankind's activity (such as power station exploitation, water pollution and illegally fish for) severe jamming, lead to Da Shi
Sturgeon population scale reduces, even in Critical Condition.For the severe situation of its population change, we carried out serial ecological and
Breeding tackling key problem research, has grasped the crucial Ecological Data of the aspects such as certain relevant its breeding, feeding habits, and it is right to have laid in more than 100
Reach the breeding reserve parent fish of age at sexual maturity.Although many effort of early stage, extensive artificial propagation and wild stockss are expanded
Increase and yet suffer from many 'bottleneck' restrictions, the artificial propagation difficulty of acipenser dabryanus is in exploratory stage early stage greatly.
Acipenser dabryanus artificial fecundation method is mainly the artificial propagation techniques with reference to similar Acipenser Sinensis and flow process at present, excessively slightly
Put, and acipenser dabryanus defines its unique the propagational ecologic habits and characteristics for adaptation zone water ecological environment during evolution, simply
The artificial propagation techniques flow process applying mechanically other Acipenser Sinensiss, effect is very undesirable, and the problem of being primarily present includes: 1. male and female are different
Step；2. seminal fluid and ovum ratio excessively extensive it is impossible to adapt to large-scale breeding technology application；3. breeding potential, incubation rate are low；4. it is fertilized
Ovum loss rate is high；5. parent's mortality rate is higher.Therefore, the physiological feature according to acipenser dabryanus and reproductive characteristic, exploitation is a set of to be had
The acipenser dabryanus artificial propagation techniques flow process of characteristic is advantageously implemented the extensive artificial propagation of whole process of acipenser dabryanus.
Content of the invention
The purpose of the present invention is for the unique physiology of acipenser dabryanus, ecosystem characterization, the exploitation distinctive acipenser dabryanus of a set of tool
Artificial propagation techniques flow process, not, rate of fertilization, incubation rate be not high to solve fineness in acipenser dabryanus artificial propagation operating process, real
Existing acipenser dabryanus fry large-scale production, provides a kind of artificial fecundation method of acipenser dabryanus.
For achieving the above object, the technical solution used in the present invention is: a kind of artificial fecundation method of acipenser dabryanus, and raun is divided
The oxytocin of double injection, double injection is spaced apart 12h, injects 1/3～1/2 for accumulated dose for the first time, and milter adopts a pin
Injection, the time is synchronous with raun first pin, and dosage is the half of raun；It is fertilized using half-dried, water, ovum, seminal fluid ratio are pressed
Mixed according to 75:50:1, stirred 2～3min.
Further, described oxytocin is lhr-a26ug+ 1.5/kg of Cyprinus carpio hypophysis cerebri.
The method have the benefit that:
(1) present invention provides the acipenser dabryanus artificial propagation techniques flow process of complete set, provides skill for extensive artificial propagation
Art supports；
(2) adopt the inventive method, can effectively distinguish the Maturity of acipenser dabryanus parent fish, and improve acipenser dabryanus parent fish and hasten parturition
Rate；By suitable compatibility of drugs, seminal fluid, ovum, the ratio of water, acipenser dabryanus incubation rate is made to reach as high as more than 85%；By adopting
Take off glutinous technology, the flow cavitation result in hatching process and triploidy rate with germ cell, solve the restrictive factor in hatching process.
Specific embodiment
Below in conjunction with the embodiment of the present invention, the technical scheme in the embodiment of the present invention is clearly and completely described,
Obviously, described embodiment is only a part of embodiment of the present invention, rather than whole embodiments.Based in the present invention
Embodiment, the every other embodiment that those of ordinary skill in the art are obtained under the premise of not making creative work, all
Belong to the scope of protection of the invention.
Embodiment 1
1st, under natural conditions, water temperature starts when reaching 16 DEG C to note active situation in pond for the acipenser dabryanus, and is worn with abdominal part
The mode in hole takes ovum to observe, and taken ovum heating is cut in the middle of animal pole after boiling and observed, and judges Da Shi using polarization index
Sturgeon breeds sequential.
2nd, before artificial propagation, temporarily support 7 days in miniflow pond, raun injects lhr-a26ug+ 1.5/kg of Cyprinus carpio hypophysis cerebri
Odinagogue, using biphasic injection, be spaced 12h, the first pin is the 1/3 of accumulated dose；Milter adopt a pin injection, the time with
Raun first pin is synchronous, and dosage is the half of raun；After pin, raun, milter are all temporarily supported in the pool of miniflow pond.(lhr-a2 is peaceful
Three lives pharmaceutcal corporation, Ltd of ripple city produces luteinizing hormone releasing hormone a2).
3rd, after the first pin injection 20h, start to check that in the pool of miniflow pond, acipenser dabryanus is from ovulation situation；According to Maturity respectively
Artificial propagation is carried out to acipenser dabryanus.
4th, semen collection, inserts gonopore with conduit, and seminal fluid accesses hermetic bag, 4 DEG C of preservations after oxygenation.
5th, cut gonoduct with self-control scalpel, gently extruding abdominal part takes ovum, using semidry method fertilization, water, ovum, seminal fluid ratio
Example is mixed according to 75:50:1, gentle agitation 2min.
6th, germ cell with clean water 1 time, with the mixing of food grade Pulvis Talci suspension, Pulvis Talci suspension ratio is 10%, stirs
Mix de- glutinous 30min after 1min.
7th, take off glutinous after with clean water 2 times, proceed to Acipenser Sinensis vial-type couveuse and hatched, put 50,000 in each hatching bottle and be subject to
Smart ovum, water rate control is in 2m3/ h, 18 DEG C of water temperature.
8th, measure rate of fertilization when incubating oosperm to little yolk plug forms the phase, every 12h inhales in hatching bottle mould ovum once.
9th, after incubating oosperm to hatching after the eye capsule formation phase, every 4h inhales in hatching bottle mould ovum once.
10th, reach after the head phase after incubating oosperm to tail, using Lotic hatching.
11st, after 1 day after fry cluster, proceed to cultivation cylinder and cultivated.
Embodiment 2
1st, under natural conditions, water temperature starts when reaching 16 DEG C to note active situation in pond for the acipenser dabryanus, and is worn with abdominal part
The mode in hole takes ovum to observe, and taken ovum heating is cut in the middle of animal pole after boiling and observed, and judges Da Shi using polarization index
Sturgeon breeds sequential.
2nd, before artificial propagation, temporarily support 9 days in miniflow pond, raun injects lhr-a26ug+ 1.5/kg of Cyprinus carpio hypophysis cerebri
Odinagogue, using biphasic injection, be spaced 12h, the first pin is the 1/2 of accumulated dose；Milter adopt a pin injection, the time with
Raun first pin is synchronous, and dosage is the half of raun；After pin, raun, milter are all temporarily supported in the pool of miniflow pond.(lhr-a2 is peaceful
Three lives pharmaceutcal corporation, Ltd of ripple city produces luteinizing hormone releasing hormone a2).
3rd, after the first pin injection 20h, start to check that in the pool of miniflow pond, acipenser dabryanus is from ovulation situation；According to Maturity respectively
Artificial propagation is carried out to acipenser dabryanus.
4th, semen collection, inserts gonopore with conduit, and seminal fluid accesses hermetic bag, 4 DEG C of preservations after oxygenation.
5th, cut gonoduct with self-control scalpel, gently extruding abdominal part takes ovum, using semidry method fertilization, water, ovum, seminal fluid ratio
Example is mixed according to 75:50:1, gentle agitation 3min.
6th, germ cell with clean water 1 time, with the mixing of food grade Pulvis Talci suspension, Pulvis Talci suspension ratio is 10%, stirs
Mix de- glutinous 30min after 1min.
7th, take off glutinous after with clean water 2 times, proceed to Acipenser Sinensis vial-type couveuse and hatched, put 50,000 in each hatching bottle and be subject to
Smart ovum, water rate control is in 3m3/ h, 18 DEG C of water temperature.
8th, measure rate of fertilization when incubating oosperm to little yolk plug forms the phase, every 12h inhales in hatching bottle mould ovum once.
9th, after incubating oosperm to hatching after the eye capsule formation phase, every 4h inhales in hatching bottle mould ovum once.
10th, reach after the head phase after incubating oosperm to tail, using Lotic hatching.
11st, after 1 day after fry cluster, proceed to cultivation cylinder and cultivated.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all essences in the present invention
Within god and principle, any modification, equivalent substitution and improvement made etc., should be included within the scope of the present invention.
Claims (1)
1. it is characterised in that raun oxytocin injection at twice, double injection is spaced apart a kind of artificial fecundation method of acipenser dabryanus
12h, injects 1/3～1/2 for accumulated dose for the first time, and milter adopts a pin injection, and the time is synchronous with raun first pin, dosage
Half for raun；Using semidry method fertilization, water, ovum, seminal fluid ratio are mixed according to 75:50:1, stir 2～3min, institute
Stating oxytocin is lhr-a2 6ug+ 1.5/kg of Cyprinus carpio hypophysis cerebri.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410428801.8A CN104145869B (en) | 2014-08-27 | 2014-08-27 | Artificial breeding method for acipenser dabryanus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410428801.8A CN104145869B (en) | 2014-08-27 | 2014-08-27 | Artificial breeding method for acipenser dabryanus |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104145869A CN104145869A (en) | 2014-11-19 |
| CN104145869B true CN104145869B (en) | 2017-01-25 |
Family
ID=51871677
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410428801.8A Active CN104145869B (en) | 2014-08-27 | 2014-08-27 | Artificial breeding method for acipenser dabryanus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104145869B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106069899B (en) * | 2016-06-15 | 2021-12-28 | 中国水产科学研究院长江水产研究所 | Method for realizing natural mating and oviposition of Acipenser dabryanus through artificial regulation |
| CN106069900B (en) * | 2016-06-15 | 2017-10-24 | 北京渔夫水产技术开发中心 | A kind of method of Europe huso sturgeon artificial propagation and carp application pituitary |
| CN110200725A (en) * | 2019-06-28 | 2019-09-06 | 中国长江三峡集团有限公司中华鲟研究所 | A kind of mandarin sturgeon hurtless measure semen collection method and device |
| CN112075365A (en) * | 2020-09-25 | 2020-12-15 | 四川省农业科学院水产研究所(四川省水产研究所) | Method for improving hatching rate of fertilized eggs of Acipenser changjiang |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101720705B (en) * | 2009-12-03 | 2012-01-25 | 中国海洋大学 | Culture method of orange adductor scallop |
| CN101999331A (en) * | 2010-11-26 | 2011-04-06 | 中国水产科学研究院长江水产研究所 | Breeding method of snakehead by artificial insemination |
| CN102113467A (en) * | 2011-03-07 | 2011-07-06 | 广州市先步农业发展有限公司 | Method for artificial fertilization of sturgeon |
| CN102165929A (en) * | 2011-06-02 | 2011-08-31 | 广州市先步农业发展有限公司 | Artificial propagation method for sturgeon |
| CN102652499A (en) * | 2012-04-25 | 2012-09-05 | 吴江市水产养殖有限公司 | Paddlefish breeding method |
-
2014
- 2014-08-27 CN CN201410428801.8A patent/CN104145869B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN104145869A (en) | 2014-11-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101940184B (en) | Breeding and culturing method of improved crucian carp | |
| CN103960184B (en) | A kind of loach industrial artificial breeding improves the seedling-cultivating method of survival rate | |
| CN104145869B (en) | Artificial breeding method for acipenser dabryanus | |
| CN105360028B (en) | The artificial fecundation method of east Triplophysa | |
| CN106577393A (en) | Artificial propagation method for breeding acrossocheilus fasciatus | |
| CN103461253B (en) | Yellow River fish artificial fecundation method | |
| CN104642212A (en) | Artificial breeding method for onychostoma simus | |
| CN104782549A (en) | Artificial carassius auratus propagation method | |
| CN104396813A (en) | Gymnocypris przewalskii full artificial breeding method | |
| CN103141411B (en) | Hippocampus erectus parent sea horse pairing method | |
| CN104255615B (en) | It is a kind of that soft thorn is naked splits buttocks fish artificial fecundation method | |
| CN101999332B (en) | Test-tube Tilapia nilotica breeding method | |
| CN103875567A (en) | Artificial spawning method for osteoglossum bicirrhosum | |
| CN110612930A (en) | Artificial breeding method for rainbow trout | |
| CN104285855A (en) | Large-scale xenocypris davidi bleeker artificial reproduction method | |
| CN103918602B (en) | A kind of high-efficiency artificial mating system of Megalobatrachus japonicus daoidianuas (Blanchard) | |
| CN106489799B (en) | The method that breeding is sheerly the full raun of Pelteobagrus fulvidraco and supermale fish large-scale production all-male fish | |
| CN106879515A (en) | A kind of viscidity-removing method of body graining embryonated egg high | |
| CN106069928A (en) | A kind of artificial fecundation method of fork-tail catfish | |
| CN106804477A (en) | A kind of artificial fecundation method of Pelteobagrus fulvidraco | |
| CN109329138A (en) | A method of improving mullet artificial breed rate and hatching rate | |
| CN109496922A (en) | A kind of producing method for seed of hydrostatic pressing induction rainbow trout tetraploid | |
| CN108541632A (en) | A kind of sturgeon mating system | |
| CN104137799A (en) | Artificial breeding method for wild lampetra morii | |
| CN110178766B (en) | Artificial breeding method of Acipenser lapipenser |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |