CN109329138A - A method of improving mullet artificial breed rate and hatching rate - Google Patents
A method of improving mullet artificial breed rate and hatching rate Download PDFInfo
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- CN109329138A CN109329138A CN201811373853.4A CN201811373853A CN109329138A CN 109329138 A CN109329138 A CN 109329138A CN 201811373853 A CN201811373853 A CN 201811373853A CN 109329138 A CN109329138 A CN 109329138A
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- 230000012447 hatching Effects 0.000 title claims abstract description 31
- 238000002347 injection Methods 0.000 claims abstract description 20
- 239000007924 injection Substances 0.000 claims abstract description 20
- 230000009027 insemination Effects 0.000 claims abstract description 15
- 235000013601 eggs Nutrition 0.000 claims abstract description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 10
- 239000005556 hormone Substances 0.000 claims abstract description 6
- 229940088597 hormone Drugs 0.000 claims abstract description 6
- 230000001737 promoting effect Effects 0.000 claims abstract description 6
- 210000004907 gland Anatomy 0.000 claims abstract description 5
- 230000001568 sexual effect Effects 0.000 claims abstract description 5
- 238000009372 pisciculture Methods 0.000 claims abstract description 4
- 102000002322 Egg Proteins Human genes 0.000 claims description 18
- 108010000912 Egg Proteins Proteins 0.000 claims description 18
- 210000004681 ovum Anatomy 0.000 claims description 17
- 230000002706 hydrostatic effect Effects 0.000 claims description 16
- 241000722870 Dicliptera chinensis Species 0.000 claims description 13
- 230000004720 fertilization Effects 0.000 claims description 13
- 239000000706 filtrate Substances 0.000 claims description 8
- 239000000463 material Substances 0.000 claims description 8
- 239000000243 solution Substances 0.000 claims description 8
- 229960004407 chorionic gonadotrophin Drugs 0.000 claims description 7
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- 108700012941 GNRH1 Proteins 0.000 claims description 6
- 239000000579 Gonadotropin-Releasing Hormone Substances 0.000 claims description 6
- 230000011712 cell development Effects 0.000 claims description 5
- 230000004992 fission Effects 0.000 claims description 5
- 230000035800 maturation Effects 0.000 claims description 5
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- 239000000431 corpus luteum hormone Substances 0.000 claims 1
- 239000003488 releasing hormone Substances 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 abstract description 3
- 238000009395 breeding Methods 0.000 abstract description 2
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- 239000013505 freshwater Substances 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
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- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
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- 102000004169 proteins and genes Human genes 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/17—Hatching, e.g. incubators
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention belongs to fishery and fish farming industry field more particularly to a kind of methods for improving mullet artificial breed rate and hatching rate.The method of the present invention includes carrying out reinforced cultivating to mullet parent, resting to parent injection promoting sexual gland hormone, constant temperature under specific temperature conditions, programmed breeding, the regulation of fertilized eggs water temperature, move pond and cultivate.Compared with common cultural method, the control of artificial insemination time is carried out to mullet using the method for the present invention, is remarkably improved its artificial breed rate and hatching rate.In addition, the method for the present invention also has many advantages, such as that easy to operate, production cost is low, easy promotion and implementation.
Description
Technical field
The invention belongs to fishery and fish farming industry field more particularly to a kind of raising mullet artificial breed rates and hatching rate
Method.
Background technique
Mullet, also known as golden mullet or barracuda are a kind of fin fish that gold mullet section Yu belongs to, civil to be also referred to as meat rod
Son, blood-shot eye illness mullet etc., are the brackish culture fingerlings of coastal area of china preciousness.Mullet can be used as fancy fishes, can also eat, meat
Matter is fresh and tender, tasty, is the delicacies in feast.It include nutrition abundant in mullet meat, in addition to a large amount of high-quality protein
It is also micro rich in unsaturated fatty acid, vitamin, cholesterol and magnesium, iron, calcium, zinc, phosphorus, selenium, potassium, copper, sodium, manganese etc. outside matter
Element, often edible mullet is beneficial to cardiovascular health, can protect cardiac muscle cell, promote blood circulation, and prevents palpitaition, heart rate
Not normal generation.
In recent years, mullet realizes fairly large artificial breeding, Shandong, Jiangsu, Zhejiang, Shanghai etc. China more
Coastal provinces and cities utilize the waters such as beach branching stream and inland river pond, propagate mullet area artificially more than 2000 hectares, averagely per acre
Produce about 750 kilograms of fish.However, yolk protein and oil droplet are filled with entire ovum mother since the micromorphology of mullet ovum is more special
Cell, therefore the mode of operation of its artificial insemination is caused to differ widely with freshwater fish and other seawater fish, mullet is manually awarded
Requirement when smart to water quality, insemination opportunity and operating condition is more harsh, nonetheless, insemination rate and hatching rate still in compared with
Low-level objectively improves cultivation difficulty, increases aquaculture cost.
The present inventor has developed a kind of raising mullet artificial breed rate and hatching rate on the basis of long-term cultivation is practiced
Method.Compared with common cultural method, artificial insemination is carried out to mullet using the method for the present invention, it is remarkably improved and manually awards
Smart rate and hatching rate.In addition, the method for the present invention also has many advantages, such as that easy to operate, production cost is low, easy promotion and implementation.
Summary of the invention
The present invention is intended to provide a kind of easy to operate, significant effect, the good raising mullet artificial breed rate of applicability and incubating
The method of rate.
The method of the present invention raising mullet artificial breed rate and hatching rate, comprising the following steps:
(1) reinforced cultivating is carried out to mullet parent to promote to strengthen, reach maturity to it；
(2) when mullet egg cell development maturation, by lower water temperature to 15-17 DEG C, promoting sexual gland hormone is injected to parent；
(3) it is primary that injection is repeated after raising 20 hours in 15-17 DEG C of constant temperature hydrostatic；
(4) when ovum fertilization hole is to be opened after raising 14 hours in 15-17 DEG C of constant temperature hydrostatic, complete coverage Fertilization Hole is beaten
It drives journey into, and completes artificial insemination in the 30-40min after ovum fertilization hole fully opens；
(5) it is hatching that control water temperature fertilized eggs after 15-17 DEG C, 20 hours, which start fission,；
Hatching is moved into Tu Chi after (6) 48 hours to cultivate, starts to feed opening material after 148-150 hours.
Further, the method for the present invention thereto (3), Dicliptera chinensis be added in constant temperature hydrostatic described in (4) step extract
Liquid, Dicliptera chinensis extracting solution additional amount are the 0.065% of constant temperature hydrostatic total volume.
Further, above-mentioned Dicliptera chinensis extracting solution is prepared by the following method: after taking Dicliptera chinensis drying, crushing plus 10 times are measured
15% ethyl alcohol, refluxing extraction 1 hour after impregnating 2 hours, filtering, filtrate is spare, and filter residue repeats to extract 2 times, and filtering merges filter
Liquid, it is 1.07-1.09 to get to Dicliptera chinensis extracting solution that filtrate is concentrated under reduced pressure into relative density under the conditions of 45 DEG C.
Preferably, promoting sexual gland hormone described in the method for the present invention (2) step is selected from least one of following compositions:
Luteinizing hormone releasing-hormone A2, chorionic gonadotrophin or compound chorionic gonadotrophin A type.
Further, the injecting method of above-mentioned Luteinizing hormone releasing-hormone A2 is to inject 2.2-2.4 μ g/kg for the first time, the
Biphasic injection 16.5-17.5 μ g/kg.
Further, the injecting method of above-mentioned chorionic gonadotrophin is to inject 45-50 unit/kg for the first time, second of injection
210-225 unit/kg.
Moreover, it relates to which the method for above-mentioned raising mullet artificial breed rate and hatching rate is in fish farming industry
Using.
To sum up, the present invention improves mullet artificial insemination method, obtain a kind of easy to operate, significant effect,
The new method of applicability good raising mullet artificial breed rate and hatching rate.Through culture experiment, can be incited somebody to action using the method for the present invention
The artificial breed rate and hatching rate of mullet are promoted to 95% or more, hence it is evident that higher than common mullet spermatozoa insemination method.In addition to this,
The method of the present invention also has many advantages, such as that easy to operate, production cost is low, easy promotion and implementation.
Specific embodiment
Illustrate embodiments of the present invention below by way of specific specific example, those skilled in the art can be by this specification
Other advantages and efficacy of the present invention can be easily understood for disclosed content.The present invention can also pass through in addition different specific realities
The mode of applying is embodied or practiced, the various details in this specification can also based on different viewpoints and application, without departing from
Various modifications or alterations are carried out under spirit of the invention.
Before further describing the specific embodiments of the present invention, it should be appreciated that protection scope of the present invention is not limited to down
State specific specific embodiment；It is also understood that term used in the embodiment of the present invention is specific specific in order to describe
Embodiment, rather than limiting the scope of protection of the present invention.
Unless otherwise defined, all technical and scientific terms and those skilled in the art of the present technique used in the present invention are usual
The meaning of understanding is identical.In addition to specific method, equipment, material used in the embodiment, according to those skilled in the art
Grasp and record of the invention to the prior art, can also use and method described in the embodiment of the present invention, equipment, material
Any method, equipment and the material of the similar or equivalent prior art realizes the present invention.
Embodiment 1
A method of improving mullet artificial breed rate and hatching rate, comprising the following steps:
(1) reinforced cultivating is carried out to mullet parent to promote to strengthen, reach maturity to it；
(2) when mullet egg cell development maturation, by lower water temperature to 15-17 DEG C, to parent injection luteotropin release
Hormone A2, injection volume are 2.3 μ g/kg；
(3) Luteinizing hormone releasing-hormone A2 of injection, note are repeated after raising 20 hours in 15-17 DEG C of constant temperature hydrostatic
The amount of penetrating is 17.0 μ g/kg；
(4) when ovum fertilization hole is to be opened after raising 14 hours in 15-17 DEG C of constant temperature hydrostatic, complete coverage Fertilization Hole is beaten
It drives journey into, and completes artificial insemination in the 30-40min after ovum fertilization hole fully opens；
(5) it is hatching that control water temperature fertilized eggs after 15-17 DEG C, 20 hours, which start fission,；
Hatching is moved into Tu Chi after (6) 48 hours to cultivate, starts to feed opening material after 148-150 hours.
Embodiment 2
A method of improving mullet artificial breed rate and hatching rate, comprising the following steps:
(1) reinforced cultivating is carried out to mullet parent to promote to strengthen, reach maturity to it；
(2) when mullet egg cell development maturation, by lower water temperature to 15-17 DEG C, to parent injection injection suede rush property
Element, injection volume are 46 units/kg；
(3) after taking Dicliptera chinensis drying, crushing plus 10 times of 15% ethyl alcohol of amount filter refluxing extraction 1 hour after immersion 2 hours,
Filtrate is spare, and filter residue repeats to extract 2 times, and filtering, merging filtrate, filtrate is concentrated under reduced pressure into relative density under the conditions of 45 DEG C and is
1.07-1.09 obtains Dicliptera chinensis extracting solution, and gained Dicliptera chinensis extracting solution is added to 15-17 DEG C according to 0.065% volume ratio
Constant temperature hydrostatic in；
(4) injection one injection chorionic gonadotrophin is repeated after raising mullet parent 20 hours in above-mentioned constant temperature hydrostatic,
Injection volume is 220 units/kg；
(5) when ovum fertilization hole is to be opened after raising mullet parent 14 hours in above-mentioned constant temperature hydrostatic, complete coverage by
Process is opened in smart hole, and completes artificial insemination in the 30-40min after ovum fertilization hole fully opens；
(6) it is hatching that control water temperature fertilized eggs after 15-17 DEG C, 20 hours, which start fission,；
Hatching is moved into Tu Chi after (7) 48 hours to cultivate, starts to feed opening material after 148-150 hours.Comparative example
A kind of mullet artificial insemination method, comprising the following steps:
(1) reinforced cultivating is carried out to mullet parent to promote to strengthen, reach maturity to it；
(2) when mullet egg cell development maturation, Luteinizing hormone releasing-hormone A2 is injected to parent, injection volume is 5.0 μ g/
kg；
(3) Luteinizing hormone releasing-hormone A2 of injection is repeated after raising 12 hours in 25 DEG C of hydrostatic, injection volume is
15.0μg/kg；
(4) raising to ovum fertilization hole is opened in 25 DEG C of hydrostatic, and complete in 2 hours after ovum fertilization hole fully opens
At artificial insemination；
It (5) is hatching to fertilized eggs fission；
Hatching is moved into great Chi after (6) 5 days to cultivate, starts to feed opening material after 7 days.
Artificial breed rate and hatching rate testing experiment
Artificial breed rate is carried out to embodiment 1-2 and comparative example method respectively and hatching rate is tested, other cultivations of each group
Condition is completely the same.
Test method: 8 hours after artificial insemination, a certain number of fish-eggs are fished for from hydrostatic with small net, are placed on ceramic whiteware disk
In checked, become turbid, whiten, the rotten bad ovum of idiosome or hollow ovum pick out counting, then calculate be fertilized it is (good
Ovum has normal fetus in ossphere) ovum number, good ovum percentage shared in total fish-egg number is artificial breed rate, hatches fry
The percentage that number accounts for fertilized eggs number is hatching rate.
Test result is as follows shown in table 1.
Each test group artificial breed rate of table 1 and hatching rate test result
It can be seen from Table 1 that compared with the common mullet spermatozoa insemination method of comparative example, it can be bright using the method for the present invention
The aobvious artificial breed rate and hatching rate for improving mullet, in comparison, the effect of 2 method of embodiment is more significant.
The preferred embodiments of the disclosure and embodiment are explained in detail above, but the present invention is not limited to
The above-described embodiment and examples can also not depart from the present invention within the knowledge of those skilled in the art
Various changes can be made under the premise of design.
Claims (7)
1. a kind of method for improving mullet artificial breed rate and hatching rate, it is characterised in that the following steps are included:
(1) reinforced cultivating is carried out to mullet parent to promote to strengthen, reach maturity to it；
(2) when mullet egg cell development maturation, by lower water temperature to 15-17 DEG C, promoting sexual gland hormone is injected to parent；
(3) it is primary that injection is repeated after raising 20 hours in 15-17 DEG C of constant temperature hydrostatic；
(4) when ovum fertilization hole is to be opened after raising 14 hours in 15-17 DEG C of constant temperature hydrostatic, complete coverage Fertilization Hole open into
Journey, and artificial insemination is completed in the 30-40min after ovum fertilization hole fully opens；
(5) it is hatching that control water temperature fertilized eggs after 15-17 DEG C, 20 hours, which start fission,；
Hatching is moved into Tu Chi after (6) 48 hours to cultivate, starts to feed opening material after 148-150 hours.
2. the method as described in claim 1, it is characterised in that thereto (3), be added in constant temperature hydrostatic described in (4) step
Dicliptera chinensis extracting solution, Dicliptera chinensis extracting solution additional amount are the 0.065% of constant temperature hydrostatic total volume.
3. method according to claim 2, wherein the Dicliptera chinensis extracting solution is prepared by the following method: take Dicliptera chinensis it is dry,
After crushing plus 10 times of 15% ethyl alcohol of amount filter refluxing extraction 1 hour after immersion 2 hours, and filtrate is spare, and filter residue repeats to extract 2
Secondary, filtering, merging filtrate, it is that 1.07-1.09 mentions to get to Dicliptera chinensis that filtrate is concentrated under reduced pressure into relative density under the conditions of 45 DEG C
Take liquid.
4. the method as described in claim 1, wherein the promoting sexual gland hormone is selected from least one of following compositions: promoting corpus luteum
Hormone-releasing hormone A2, chorionic gonadotrophin or compound chorionic gonadotrophin A type.
5. method as claimed in claim 4, wherein the injecting method of the Luteinizing hormone releasing-hormone A2 is to inject for the first time
2.2-2.4 μ g/kg, second of injection 16.5-17.5 μ g/kg.
6. method as claimed in claim 4, wherein the injecting method of the chorionic gonadotrophin be for the first time injection 45-50 unit/
Kg, second of injection 210-225 unit/kg.
7. the method for raising mullet artificial breed rate as claimed in any one of claims 1 to 6 and hatching rate is in fish farming industry
Application.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811373853.4A CN109329138B (en) | 2018-11-19 | 2018-11-19 | Method for improving artificial insemination rate and hatching rate of pike |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201811373853.4A CN109329138B (en) | 2018-11-19 | 2018-11-19 | Method for improving artificial insemination rate and hatching rate of pike |
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| CN109329138A true CN109329138A (en) | 2019-02-15 |
| CN109329138B CN109329138B (en) | 2021-05-04 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111084131A (en) * | 2019-12-26 | 2020-05-01 | 上海市水产研究所（上海市水产技术推广站） | Artificial breeding method for ground-based self-production of pike |
| CN111387095A (en) * | 2020-04-29 | 2020-07-10 | 黄骅市宏润水产养殖有限公司 | Breeding method for improved variety parent of mullet |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101324904B1 (en) * | 2012-05-24 | 2013-11-04 | 전라남도 | Aquaculture method for coreoperca use a assorted feed |
| CN105557578A (en) * | 2015-12-19 | 2016-05-11 | 太和县顺发养殖有限公司 | Method for improving hatching rate and hatching speed of catfish oosperms |
| CN106973953A (en) * | 2017-05-23 | 2017-07-25 | 浦北县富通电子科技有限公司 | Electronic product thimerosal |
| CN107711621A (en) * | 2017-11-07 | 2018-02-23 | 华南师范大学 | A kind of mating system of Scatophagus argus (Linnaeus) |
| CN110537509A (en) * | 2019-08-14 | 2019-12-06 | 天津蕴华农业科技发展有限公司 | incubation and cultivation method of loaches |
-
2018
- 2018-11-19 CN CN201811373853.4A patent/CN109329138B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101324904B1 (en) * | 2012-05-24 | 2013-11-04 | 전라남도 | Aquaculture method for coreoperca use a assorted feed |
| CN105557578A (en) * | 2015-12-19 | 2016-05-11 | 太和县顺发养殖有限公司 | Method for improving hatching rate and hatching speed of catfish oosperms |
| CN106973953A (en) * | 2017-05-23 | 2017-07-25 | 浦北县富通电子科技有限公司 | Electronic product thimerosal |
| CN107711621A (en) * | 2017-11-07 | 2018-02-23 | 华南师范大学 | A kind of mating system of Scatophagus argus (Linnaeus) |
| CN110537509A (en) * | 2019-08-14 | 2019-12-06 | 天津蕴华农业科技发展有限公司 | incubation and cultivation method of loaches |
Non-Patent Citations (4)
| Title |
|---|
| 吴颖瑞等: "１５３种中草药对罗非鱼无乳链球菌和海豚链球菌的", 《西北农林科技大学学报（自然科学版）》 * |
| 广西壮族自治区农业厅等: "《动物高效益养殖及加工》", 31 May 1991, 广西科学技术出版社 * |
| 李明德: "《水生生物与养殖（包括淡水、咸淡水、海洋）》", 30 June 1990, 南开大学出版社 * |
| 王玉堂: "《淡水名特优良种实用养殖技术大全》", 30 September 1990, 海洋出版社 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111084131A (en) * | 2019-12-26 | 2020-05-01 | 上海市水产研究所（上海市水产技术推广站） | Artificial breeding method for ground-based self-production of pike |
| CN111084131B (en) * | 2019-12-26 | 2021-08-20 | 上海市水产研究所（上海市水产技术推广站） | Artificial breeding method for ground-based self-production of pike |
| CN111387095A (en) * | 2020-04-29 | 2020-07-10 | 黄骅市宏润水产养殖有限公司 | Breeding method for improved variety parent of mullet |
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Effective date of registration: 20231102 Address after: 224232 South East Head of Shuangyang Zha, Linhai Town, Sheyang County, Yancheng City, Jiangsu Province Patentee after: Jiangsu Haixin Aquatic Original Seed Farm Technology Co.,Ltd. Patentee after: SHEYANG COUNTY DAYANG SPECIAL AQUATIC PRODUCT SEEDLING FIELD Address before: 224000 southeast head of Shuangyang sluice, Jianhai Town, Sheyang City, Jiangsu Province Patentee before: YANCHENG JINYANG AQUATIC FARM Patentee before: SHEYANG COUNTY DAYANG SPECIAL AQUATIC PRODUCT SEEDLING FIELD |