CN103918602B - A kind of high-efficiency artificial mating system of Megalobatrachus japonicus daoidianuas (Blanchard) - Google Patents
A kind of high-efficiency artificial mating system of Megalobatrachus japonicus daoidianuas (Blanchard) Download PDFInfo
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- CN103918602B CN103918602B CN201410155454.6A CN201410155454A CN103918602B CN 103918602 B CN103918602 B CN 103918602B CN 201410155454 A CN201410155454 A CN 201410155454A CN 103918602 B CN103918602 B CN 103918602B
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Abstract
The high-efficiency artificial mating system of a kind of Megalobatrachus japonicus daoidianuas (Blanchard), relates to Megalobatrachus japonicus daoidianuas (Blanchard) cultural technique field, including techniques below method: modulation promote the sexual maturity injection, by maturity carry out classification injection, manually combine germ cell, clean germ cell, hatchery hatching.The present invention combines pure artificial propagation and pure imitative wild breeding, solves the bad and pure imitative wild breeding nonsynchronous difficult problem of male and female fish of pure artificial propagation sexual development, and the method using both to combine improves reproductive efficiency and the quality of Megalobatrachus japonicus daoidianuas (Blanchard).
Description
Technical field:
The present invention relates to Megalobatrachus japonicus daoidianuas (Blanchard) cultural technique field, be specifically related to the high-efficiency artificial side of breeding of a kind of Megalobatrachus japonicus daoidianuas (Blanchard)
Method.
Background technology:
Megalobatrachus japonicus daoidianuas (Blanchard) is commonly called as Andrias davidianus Blanchard, be the most existing maximum be also most precious Amphibian, crying of it
Sound is like child's sob, and therefore people are again its " Megalobatrachus japonicus daoidianuas (Blanchard) ", is national two class protection aqua-marine lifes,
Being agriculture industrialization and featured agriculture focus development kind, be wild animal gene protection kind, China is big
Salamander is in addition to Xinjiang, Tibet, Inner Mongol, Taiwan have no report, and there is distribution remaining provinces and regions, mainly originates in length
In the mountain stream streams in river, the Yellow River and Zhujiang River middle and upper reaches tributary, Andrias davidianus Blanchard is the most all hidden and occupies between the stone gap of mountain stream,
Cave is positioned at below the water surface, and night is quiet keeps in the mouth clitter of beach, once finds that prey, through out-of-date, is just carried out
Surprise attack, because the tooth in its mouth is the sharpest but also close, in prey inlet port after be difficult to run away.Megalobatrachus japonicus daoidianuas (Blanchard) has
The strongest resistance to hungry ability, raising does not takes food for two, three years in refrigerant water also will not die of hunger.It is the most also
Energy gluttony, make a hearty meal 1/5th can put on weight, and during deprivation of food, there is also cannibalism
Phenomenon, even allay one's hunger with ovum, due to the breeding potential of life habit Megalobatrachus japonicus daoidianuas (Blanchard) of these uniquenesses of Megalobatrachus japonicus daoidianuas (Blanchard)
The highest, and due in the ornamental value of Megalobatrachus japonicus daoidianuas (Blanchard) and the highest market of edibility to Megalobatrachus japonicus daoidianuas (Blanchard)
Demand big especially, therefore people begin one's study large-scale artificial cultivation Megalobatrachus japonicus daoidianuas (Blanchard), and carrying out artificial
The when of cultivation Megalobatrachus japonicus daoidianuas (Blanchard), have employed a variety of way and Megalobatrachus japonicus daoidianuas (Blanchard) is carried out artificial propagation, including pure
Artificial propagation and pure imitative wild breeding, but both propagation methods are to there being respective shortcoming, the purest people
Work breeding Megalobatrachus japonicus daoidianuas (Blanchard) sexual development is bad, and pure imitative wild breeding then there will be the nonsynchronous difficult problem of male and female fish.
Summary of the invention:
It is bad that the technical problem to be solved is to provide one to solve pure artificial propagation sexual development
An imitative wild breeding male and female fish nonsynchronous difficult problem pure with Megalobatrachus japonicus daoidianuas (Blanchard), and the baby of breeding potential can be effectively improved
The high-efficiency artificial mating system of baby fish.
The technical problem to be solved uses following technical scheme to realize:
A kind of high-efficiency artificial mating system of Megalobatrachus japonicus daoidianuas (Blanchard), including techniques below method:
1, annual mid-August, Megalobatrachus japonicus daoidianuas (Blanchard) proceeded by the sexual maturity stage when, Megalobatrachus japonicus daoidianuas (Blanchard) is become according to property
Ripe different situations and the male and female of Megalobatrachus japonicus daoidianuas (Blanchard) are classified, by separated for male and female Megalobatrachus japonicus daoidianuas (Blanchard) complete during classification
Under conditions of full lucifuge, then observe the stagnant hole of doll's fish fillet when the stagnant hole of row is aubergine and sounds out the people in a given scope one by one in order to break a criminal case with hands stagnant
Pin of promoting the sexual maturity can be injected, the when of can naturally decontroling, in Shi Paizhi hole, hole；
2, injection of promoting the sexual maturity is allocated: by HCG:10000miu/ml, No. 2 10 micrograms of induced ovulation/prop up and 2 millis
The normal saline of liter 0.9% is mutually mixed to stir makes injection of promoting the sexual maturity；
3, after injection of promoting the sexual maturity has been modulated, carry the male Megalobatrachus japonicus daoidianuas (Blanchard) a few days ago satisfied injection required and carry out
Injecting pin of promoting the sexual maturity, the metering of injection is the injection of promoting the sexual maturity of per kilogram Megalobatrachus japonicus daoidianuas (Blanchard) injection 0.4ml；Both at note
Needing before penetrating to weigh Megalobatrachus japonicus daoidianuas (Blanchard), inject Megalobatrachus japonicus daoidianuas (Blanchard) according to weight, female Megalobatrachus japonicus daoidianuas (Blanchard) then exists
Inject in time in the case of meeting condition；
4, after pin is promoted the sexual maturity in injection, female crying of a child fish must be artificial germ cell timely within 90 hours
Extract out, be placed in dry vessel；
5, then extrude the sperm of milter with hands, and use microscopy motility of sperm, select enough vigor
Sperm, uniformly drops in the sperm of male Megalobatrachus japonicus daoidianuas (Blanchard) on the germ cell of the female Megalobatrachus japonicus daoidianuas (Blanchard) of extraction in advance；
6, after dripping off sperm five minutes, add the normal saline of 0.9% germ cell be totally submerged, after submergence ten
Within five minutes, complete fertilization；
7, repeatedly germ cell is rinsed 5 times, to rinse out unnecessary sperm completely with clear water after being fertilized；
8, after rinsing well, germ cell being placed in hatchery, checking germ cell after 24 hours, if having mould
Change, floating, variable color, shrivelled germ cell to be cleared up in time；
9, can hatch at about 40 days after cleaning.
Another technical essential is that above all of hatching operation is desirable that under conditions of 15-20 DEG C and wants
Complete under conditions of complete lucifuge.
In the whole artificial breeding stage, not only make use of pure artificial breeding technology colleague also by pure imitative wild numerous
The technology of educating combines, and directly germ cell can be placed in the hatchery in field, be not required to after carrying out copulation
Carry out special hatchery facility, and by observing manually differentiating Megalobatrachus japonicus daoidianuas (Blanchard) Maturity when
Arrange stagnant hole and use the mode touched of hands can put forward high-resolution accuracy rate, than traditional single observation
Mode more can observe the Maturity of Megalobatrachus japonicus daoidianuas (Blanchard) accurately, and is carrying out what sperm and germ cell be combined with each other
During first germ cell is placed on dry vessel in then sperm is uniformly dropped on germ cell, then
Being immersed into timely in the normal saline of 0.9%, this survival rate that can not only improve Megalobatrachus japonicus daoidianuas (Blanchard) also can be right
Germ cell and sperm carry out sterilizing thus improve the hatching quality of Megalobatrachus japonicus daoidianuas (Blanchard), the most so pass through high-efficiency artificial
The Megalobatrachus japonicus daoidianuas (Blanchard) that raising technology breeds out is higher to the adaptation ability of the conditions of existence in field, and resistance against diseases is also
More preferably, and breed that the time is short, survival rate is high compared to other techniques, Megalobatrachus japonicus daoidianuas (Blanchard) is bred and cultivates
Serve the promotion of matter.
The invention has the beneficial effects as follows: pure artificial propagation and pure imitative wild breeding are combined, solves pure people
Work reproductive ability grows the bad and pure imitative wild breeding nonsynchronous difficult problem of male and female fish, uses both to combine
Method improves reproductive efficiency and the quality of Megalobatrachus japonicus daoidianuas (Blanchard).
Detailed description of the invention:
For the technological means making the present invention realize, creation characteristic, reach purpose and effect and be readily apparent from
Solve, below in conjunction with concrete example, the present invention is expanded on further.
Specific embodiment:
1, allotment is promoted the sexual maturity injection: by HCG:10000miu/ml, No. 2 10 micrograms of induced ovulation/and 2
The normal saline of milliliter 0.9% is mutually mixed to stir makes injection of promoting the sexual maturity；
2, then annual mid-August, Megalobatrachus japonicus daoidianuas (Blanchard) proceeded by the sexual maturity stage when to Megalobatrachus japonicus daoidianuas (Blanchard) according to
The male and female of sexually matured different situations and Megalobatrachus japonicus daoidianuas (Blanchard) are classified, by separated for male and female Megalobatrachus japonicus daoidianuas (Blanchard) during classification
Under conditions of complete lucifuge, then observe the stagnant hole of doll's fish fillet when the stagnant hole of row is aubergine and touches with hands
Arrange the when that Shi Paizhi hole, stagnant hole can being decontroled naturally and can inject pin of promoting the sexual maturity；
3, after injection of promoting the sexual maturity has been modulated, carry the male Megalobatrachus japonicus daoidianuas (Blanchard) a few days ago satisfied injection required and enter
Row injection is promoted the sexual maturity pin, and the injection of promoting the sexual maturity of 0.4ml is injected in the metering of injection for per kilogram Megalobatrachus japonicus daoidianuas (Blanchard)；Both existed
Needing before injection to weigh Megalobatrachus japonicus daoidianuas (Blanchard), inject Megalobatrachus japonicus daoidianuas (Blanchard) according to weight, female Megalobatrachus japonicus daoidianuas (Blanchard) is then
Inject in time in the case of meeting condition；
4, after pin is promoted the sexual maturity in injection, female crying of a child fish must be artificial germ cell timely within 90 hours
Extract out, be placed in dry vessel；
5, then extrude the sperm of milter with hands, and use microscopy motility of sperm, select enough vigor
Sperm, the sperm of male Megalobatrachus japonicus daoidianuas (Blanchard) is uniformly dropped on the germ cell of the in advance female Megalobatrachus japonicus daoidianuas (Blanchard) of extraction；
6, after dripping off sperm five minutes, add the normal saline of 0.9% germ cell be totally submerged, after submergence
Within 15 minutes, complete fertilization；
7, repeatedly germ cell is rinsed 5 times, to rinse out unnecessary sperm completely with clear water after being fertilized；
8, after rinsing well, germ cell being placed in hatchery, checking germ cell after 24 hours, if had
Go mouldy, float, variable color, shrivelled germ cell to be cleared up in time；
9, can hatch at about 40 days after cleaning.
Following table is for breeding time experimental data contrast table:
| Repoductive time | Breeding quality (the 100 tail amount of surviving) | Development condition | |
| High-efficiency artificial raising technology | About 40 days | More than 95 | Good |
| Pure imitative wild breeding | About 50 days | 75-80 | Good |
| Pure artificial propagation | About 45 days | 85-95 | Good |
Following table is for breeding mass experiment Data Comparison table:
| Every 100 composition ratios alive | Field condition viability | Health degree | |
| High-efficiency artificial raising technology | 95% | By force | Excellent |
| Pure imitative wild breeding | 76% | By force | Good |
| Pure artificial propagation | 85% | Good | Good |
The ultimate principle of the present invention and principal character and advantages of the present invention have more than been shown and described.One's own profession
Skilled person will appreciate that of industry, the present invention is not restricted to the described embodiments, above-described embodiment and explanation
The principle that the present invention is simply described described in book, without departing from the spirit and scope of the present invention,
The present invention also has various changes and modifications, and these changes and improvements both fall within claimed invention model
In enclosing.Claimed scope is defined by appending claims and equivalent thereof.
Claims (2)
1. the high-efficiency artificial mating system of a Megalobatrachus japonicus daoidianuas (Blanchard), it is characterised in that: include techniques below method:
(1), Megalobatrachus japonicus daoidianuas (Blanchard) is classified according to the male and female of sexually matured different situations and Megalobatrachus japonicus daoidianuas (Blanchard) when that Megalobatrachus japonicus daoidianuas (Blanchard) proceeding by the sexual maturity stage in annual mid-August, during classification by separated for male and female Megalobatrachus japonicus daoidianuas (Blanchard) under conditions of complete lucifuge, then observe the stagnant hole of doll's fish fillet, be aubergine when arranging stagnant hole and sound out the people in a given scope one by one in order to break a criminal case the when that Shi Paizhi hole, stagnant hole can being decontroled naturally with hands and can inject pin of promoting the sexual maturity；
(2), allotment is promoted the sexual maturity injection: by HCG:10000miu/ml, No. 2 10 micrograms of induced ovulation/and the normal saline of 2 milliliter 0.9% be mutually mixed to stir and make injection of promoting the sexual maturity；
(3), after injection of promoting the sexual maturity has been modulated, carry the male Megalobatrachus japonicus daoidianuas (Blanchard) a few days ago satisfied injection required to carry out injection and promote the sexual maturity pin, need before the injection Megalobatrachus japonicus daoidianuas (Blanchard) is weighed, according to weight, Megalobatrachus japonicus daoidianuas (Blanchard) is injected, the metering of injection is the injection of promoting the sexual maturity of per kilogram Megalobatrachus japonicus daoidianuas (Blanchard) injection 0.4ml, and female Megalobatrachus japonicus daoidianuas (Blanchard) is then injected in the case of meeting condition in time；
(4), injection promote the sexual maturity after pin, female Megalobatrachus japonicus daoidianuas (Blanchard) must manually be extracted germ cell out within 90 hours timely, is placed in dry vessel；
(5), then with the sperm of hands extrusion milter, and use microscopy motility of sperm, select the sperm of enough vigor, the sperm of male Megalobatrachus japonicus daoidianuas (Blanchard) is uniformly dropped on the germ cell of the female Megalobatrachus japonicus daoidianuas (Blanchard) extracted out in advance；
(6), after dripping off sperm five minutes, add the normal saline of 0.9% germ cell is totally submerged, within after submergence 15 minutes, complete fertilization；
(7) repeatedly germ cell is rinsed 5 times, to rinse out unnecessary sperm completely with clear water after, being fertilized；
(8), rinse well after germ cell is placed in hatchery, check germ cell after 24 hours, if having go mouldy, float, variable color, shrivelled germ cell to be cleared up in time；
(9), can hatch at about 40 days after cleaning.
The high-efficiency artificial mating system of a kind of Megalobatrachus japonicus daoidianuas (Blanchard) the most according to claim 1, it is characterised in that: all of breeding operation is desirable that under conditions of 15-20 DEG C and to complete under conditions of complete lucifuge.
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| CN104430169B (en) * | 2014-12-12 | 2016-08-31 | 永新县水产站 | A kind of method promoting On The Gonadal Development of Megalobatrachus Davidianus to improve breeding three rates |
| CN104542473A (en) * | 2014-12-31 | 2015-04-29 | 重庆市杰亚大鲵养殖有限公司 | Ecological breeding method of giant salamanders |
| CN104737977A (en) * | 2015-03-10 | 2015-07-01 | 湖北宜都渔洋大鲵开发有限公司 | Artificial spawning inducing and inseminating method for giant salamander |
| CN104969894A (en) * | 2015-08-10 | 2015-10-14 | 苏州市相城区阳澄湖镇剑美水产生态养殖专业合作社 | Artificial breeding method of giant salamanders |
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| CN100490638C (en) * | 2003-11-06 | 2009-05-27 | 斗门金鲵野生动物养殖有限公司 | Method for synchronously developing gonads of salamander parents |
| CN101720720B (en) * | 2010-01-04 | 2012-03-28 | 岳西县野山土特产贸易有限公司 | Method for artificial induction breeding and biomimetic breeding of andrias davidiamus |
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