CN108684604A - A kind of artificial cultivating method of the giant salamander - Google Patents

Abstract

The invention discloses a kind of artificial cultivating methods of the giant salamander, this method is in the entire artificial breeding stage, pure artificial breeding technology colleague is not only utilized also to be combined pure imitated wild breeding technology, directly fertilized eggs can be placed in the hatchery in field after being mated, it need not carry out special hatchery facility, the survival rate of the giant salamander, which can not only be improved, can also sterilize fertilized eggs and sperm to improve the hatching quality of the giant salamander, the giant salamander for not only breeding out by high-efficiency artificial raising technology in this way is stronger to the adaptability of the life condition in field, disease resistance is also more preferable, and the breeding time is short compared to other techniques, survival rate is high, breeding and cultivation to the giant salamander play the promotion of matter.The present invention is combined pure artificial propagation and pure imitative wild breeding, solves the bad and pure imitative wild nonsynchronous problem of breeding male and female fish of pure artificial propagation sexual development, the reproductive efficiency and quality of the giant salamander are improved using the method that the two is combined.

Description

A kind of artificial cultivating method of the giant salamander

Technical field

The present invention relates to a kind of artificial cultivating methods of the giant salamander.

Background technology

The giant salamander is commonly called as giant salamander, be in the world it is existing it is maximum be also most precious amphibian, its cry is like children Vagitus sound, therefore people are called its " giant salamander ", are national two classes protection aqua-marine lifes, are agriculture industrialization and characteristic agriculture Industry focus development kind, is wild animal gene protection kind, and Chinese giant salamander, Andrias davidianus has not been reported except Xinjiang, Tibet, Inner Mongol, Taiwan Outside, remaining provinces and regions is all distributed, and is mainly originated in the mountain stream streams in the Changjiang river, the Yellow River and Zhujiang River middle and upper reaches tributary, and giant salamander is generally all Residence hide between the stone gap of mountain stream, cave be located at the water surface hereinafter, night it is quiet keep in mouthful clitter of beach, when discovery prey passes through, Just surprise attack is carried out, because the tooth in its mouth is not only sharp but also close, rear in prey inlet port is difficult to run away.The giant salamander has very strong resistance to Hungry ability, raising are not fed and will not be died of hunger for two, three years in refrigerant water.It simultaneously also can gluttony, making a hearty meal can increase / 5th of weight, when deprivation of food, the phenomenon that there is also cannibalism, or even allayed one's hunger with ovum, due to this of the giant salamander The breeding potential of a little unique life habit giant salamanders is not high, and ornamental value due to the giant salamander and edible value are all special It is high especially not big to the demand of the giant salamander in the market, therefore people begin one's study the large-scale artificial breeding giant salamander, and into When the row artificial breeding giant salamander, uses a variety of methods and artificial propagation is carried out to the giant salamander, including pure artificial breeding It grows and imitates wild breeding with pure, but both propagation methods are respective to having the shortcomings that, wherein pure artificial propagation giant salamander sexual development It is bad, and pure imitative wild breeding then will appear the nonsynchronous problem of male and female fish.

Invention content

Place in order to overcome the deficiencies of the prior art, the present invention provide one kind and solve that pure artificial propagation sexual development is bad and baby Baby fish is pure to imitate the wild nonsynchronous problem of breeding male and female fish, and can effectively improve the artificial breeding side of the giant salamander of breeding potential Method.

The present invention is achieved by the following technical solutions:

A kind of artificial cultivating method of the giant salamander, includes the following steps:

(1) when annual giant salamander mid-August proceeds by the sexal maturity stage to the giant salamander according to it is sexually matured not Male and female with situation and the giant salamander are classified, and when classification is separated under conditions of being protected from light completely by the male and female giant salamander, so Observation doll's steck stagnant hole afterwards, when arranging stagnant hole in aubergine and the holes stagnant hole Shi Paizhi of being sounded out the people in a given scope one by one in order to break a criminal case with hand can decontrol naturally Needle of promoting the sexual maturity can be injected;

(2) injection of promoting the sexual maturity is allocated:By HCG:No. 2 12000miu/ml, ovulation induction 12-14 micrograms/and 3-5 milliliters The physiological saline of 0.7-0.9%, which is mutually mixed to stir evenly, is made injection of promoting the sexual maturity;

(3) it after the completion of injection modulation of promoting the sexual maturity, carries and a few days ago being injected to meeting the male giant salamander that injection requires It promotes the sexual maturity needle, needs to weigh to the giant salamander before the injection, the giant salamander is injected according to weight, the metering of injection is per public The injection of promoting the sexual maturity of jin giant salamander injection 0.5-0.7ml, the female giant salamander are then injected in the case where meeting condition in time;

(4) after needle is promoted the sexual maturity in injection, the female giant salamander timely must manually extract fertilized eggs out within 85 hours, put In dry vessel;

(5) and then with hand the sperm for squeezing out milter, is used in combination microexamination sperm motility, selects the sperm of enough vigor, The sperm of the male giant salamander is uniformly dropped on the fertilized eggs for the female giant salamander extracted out in advance;

(6) fertilized eggs are totally submerged by the physiological saline of addition 0.7-0.9% after dripping off sperm five minutes, ten after submergence Complete fertilization within five minutes;

(7) fertilized eggs are rinsed 5-7 times with clear water repeatedly after the completion of fertilization, to rinse out extra sperm completely;

(8) fertilized eggs are placed in hatchery after rinsing well, fertilized eggs are checked after 20 hours, if there is going mouldy, on Floating, discoloration, shrivelled fertilized eggs will be cleared up in time;

(9) it can hatch completion at 30-35 days after clearing up.

The beneficial effects of the invention are as follows:In the entire artificial breeding stage, pure artificial breeding technology colleague is not only utilized Pure imitated wild breeding technology is combined, directly fertilized eggs can be placed in the hatchery in field after being mated, be not required to Special hatchery facility is carried out, and by the stagnant hole of the row of observation and is adopted when carrying out artificial resolution giant salamander maturity High-resolution accuracy rate can be put forward with the mode that hand is touched, the mode than traditional single observation more can accurately observe the giant salamander Maturity, and first fertilized eggs are placed in dry vessel so during carrying out sperm and fertilized eggs and be combineding with each other Sperm is uniformly dropped on fertilized eggs afterwards, is then timely immersed into physiological saline, this can not only improve the giant salamander Survival rate can also sterilize fertilized eggs and sperm to improve the hatching quality of the giant salamander, not only pass through high-efficiency artificial in this way The giant salamander that raising technology breeds out is stronger to the adaptability of the life condition in field, and disease resistance is also more preferable, Er Qieyu Other technologies are short compared to the breeding time, survival rate is high, and breeding and cultivation to the giant salamander play the promotion of matter.The present invention is pure Artificial propagation and pure imitative wild breeding are combined, and solve the bad and pure imitative wild breeding male and female fish of pure artificial propagation sexual development not Synchronous problem improves the reproductive efficiency and quality of the giant salamander using the method that the two is combined.

Specific implementation mode

Below in conjunction with specific embodiment, the present invention will be described in detail, herein illustrative examples and explanation of the invention For explaining the present invention, but it is not as a limitation of the invention.

Embodiment 1

A kind of artificial cultivating method of the giant salamander, includes the following steps:

(1) when annual giant salamander mid-August proceeds by the sexal maturity stage to the giant salamander according to it is sexually matured not Male and female with situation and the giant salamander are classified, and when classification is separated under conditions of being protected from light completely by the male and female giant salamander, so Observation doll's steck stagnant hole afterwards, when arranging stagnant hole in aubergine and the holes stagnant hole Shi Paizhi of being sounded out the people in a given scope one by one in order to break a criminal case with hand can decontrol naturally Needle of promoting the sexual maturity can be injected;

(2) injection of promoting the sexual maturity is allocated:By HCG:No. 2 12000miu/ml, ovulation induction 12 micrograms/and 3 milliliter 0.7% Physiological saline, which is mutually mixed to stir evenly, is made injection of promoting the sexual maturity;

(3) it after the completion of injection modulation of promoting the sexual maturity, carries and a few days ago being injected to meeting the male giant salamander that injection requires It promotes the sexual maturity needle, needs to weigh to the giant salamander before the injection, the giant salamander is injected according to weight, the metering of injection is per public The injection of promoting the sexual maturity of jin giant salamander injection 0.5ml, the female giant salamander are then injected in the case where meeting condition in time;

(4) after needle is promoted the sexual maturity in injection, the female giant salamander timely must manually extract fertilized eggs out within 85 hours, put In dry vessel;

(5) and then with hand the sperm for squeezing out milter, is used in combination microexamination sperm motility, selects the sperm of enough vigor, The sperm of the male giant salamander is uniformly dropped on the fertilized eggs for the female giant salamander extracted out in advance;

(6) fertilized eggs are totally submerged by the physiological saline of addition 0.7% after dripping off sperm five minutes, 15 points after submergence Clock completes fertilization;

(7) fertilized eggs are rinsed 5 times with clear water repeatedly after the completion of fertilization, to rinse out extra sperm completely;

(8) fertilized eggs are placed in hatchery after rinsing well, fertilized eggs are checked after 20 hours, if there is going mouldy, on Floating, discoloration, shrivelled fertilized eggs will be cleared up in time;

(9) it can hatch completion at 30 days after clearing up.

Embodiment 2

A kind of artificial cultivating method of the giant salamander, includes the following steps:

(1) when annual giant salamander mid-August proceeds by the sexal maturity stage to the giant salamander according to it is sexually matured not Male and female with situation and the giant salamander are classified, and when classification is separated under conditions of being protected from light completely by the male and female giant salamander, so Observation doll's steck stagnant hole afterwards, when arranging stagnant hole in aubergine and the holes stagnant hole Shi Paizhi of being sounded out the people in a given scope one by one in order to break a criminal case with hand can decontrol naturally Needle of promoting the sexual maturity can be injected;

(2) injection of promoting the sexual maturity is allocated:By HCG:No. 2 12000miu/ml, ovulation induction 14 micrograms/and 5 milliliter 0.9% Physiological saline, which is mutually mixed to stir evenly, is made injection of promoting the sexual maturity;

(3) it after the completion of injection modulation of promoting the sexual maturity, carries and a few days ago being injected to meeting the male giant salamander that injection requires It promotes the sexual maturity needle, needs to weigh to the giant salamander before the injection, the giant salamander is injected according to weight, the metering of injection is per public The injection of promoting the sexual maturity of jin giant salamander injection 0.7ml, the female giant salamander are then injected in the case where meeting condition in time;

(4) after needle is promoted the sexual maturity in injection, the female giant salamander timely must manually extract fertilized eggs out within 85 hours, put In dry vessel;

(5) and then with hand the sperm for squeezing out milter, is used in combination microexamination sperm motility, selects the sperm of enough vigor, The sperm of the male giant salamander is uniformly dropped on the fertilized eggs for the female giant salamander extracted out in advance;

(6) fertilized eggs are totally submerged by the physiological saline of addition 0.9% after dripping off sperm five minutes, 15 points after submergence Clock completes fertilization;

(7) fertilized eggs are rinsed 7 times with clear water repeatedly after the completion of fertilization, to rinse out extra sperm completely;

(8) fertilized eggs are placed in hatchery after rinsing well, fertilized eggs are checked after 20 hours, if there is going mouldy, on Floating, discoloration, shrivelled fertilized eggs will be cleared up in time;

(9) it can hatch completion at 35 days after clearing up.

Embodiment 3

A kind of artificial cultivating method of the giant salamander, includes the following steps:

(1) when annual giant salamander mid-August proceeds by the sexal maturity stage to the giant salamander according to it is sexually matured not Male and female with situation and the giant salamander are classified, and when classification is separated under conditions of being protected from light completely by the male and female giant salamander, so Observation doll's steck stagnant hole afterwards, when arranging stagnant hole in aubergine and the holes stagnant hole Shi Paizhi of being sounded out the people in a given scope one by one in order to break a criminal case with hand can decontrol naturally Needle of promoting the sexual maturity can be injected;

(2) injection of promoting the sexual maturity is allocated:By HCG:No. 2 12000miu/ml, ovulation induction 13 micrograms/and 4 milliliter 0.8% Physiological saline, which is mutually mixed to stir evenly, is made injection of promoting the sexual maturity;

(3) it after the completion of injection modulation of promoting the sexual maturity, carries and a few days ago being injected to meeting the male giant salamander that injection requires It promotes the sexual maturity needle, needs to weigh to the giant salamander before the injection, the giant salamander is injected according to weight, the metering of injection is per public The injection of promoting the sexual maturity of jin giant salamander injection 0.6ml, the female giant salamander are then injected in the case where meeting condition in time;

(4) after needle is promoted the sexual maturity in injection, the female giant salamander timely must manually extract fertilized eggs out within 85 hours, put In dry vessel;

(5) and then with hand the sperm for squeezing out milter, is used in combination microexamination sperm motility, selects the sperm of enough vigor, The sperm of the male giant salamander is uniformly dropped on the fertilized eggs for the female giant salamander extracted out in advance;

(6) fertilized eggs are totally submerged by the physiological saline of addition 0.8% after dripping off sperm five minutes, 15 points after submergence Clock completes fertilization;

(7) fertilized eggs are rinsed 6 times with clear water repeatedly after the completion of fertilization, to rinse out extra sperm completely;

(8) fertilized eggs are placed in hatchery after rinsing well, fertilized eggs are checked after 20 hours, if there is going mouldy, on Floating, discoloration, shrivelled fertilized eggs will be cleared up in time;

(9) it can hatch completion at 33 days after clearing up.

Claims (1)

1. a kind of artificial cultivating method of the giant salamander, which is characterized in that include the following steps:

(1) giant salamander is not sympathized with according to sexually matured when annual giant salamander mid-August proceeds by the sexal maturity stage Condition and the male and female of the giant salamander are classified, and when classification is separated under conditions of being protected from light completely by the male and female giant salamander, then sees The stagnant hole of doll's steck is examined, it can when arranging stagnant hole in aubergine and the holes stagnant hole Shi Paizhi of being sounded out the people in a given scope one by one in order to break a criminal case with hand can decontrol naturally To inject needle of promoting the sexual maturity;

(2) injection of promoting the sexual maturity is allocated:By HCG:No. 2 12000miu/ml, ovulation induction 12-14 micrograms/and 3-5 milliliters of 0.7- 0.9% physiological saline, which is mutually mixed to stir evenly, is made injection of promoting the sexual maturity;

(3) it after the completion of injection modulation of promoting the sexual maturity, carries a few days ago the male giant salamander for meeting injection requirement inject and promote the sexual maturity Needle, needs to weigh to the giant salamander before the injection, is injected to the giant salamander according to weight, and the metering of injection is per kilogram baby Baby fish injects the injection of promoting the sexual maturity of 0.5-0.7ml, and the female giant salamander is then injected in the case where meeting condition in time;

(4) after needle is promoted the sexual maturity in injection, the female giant salamander timely must manually extract fertilized eggs out within 85 hours, be placed on dry In dry vessel;

(5) and then with hand the sperm for squeezing out milter, is used in combination microexamination sperm motility, selects the sperm of enough vigor, will be male The sperm of the property giant salamander uniformly drops on the fertilized eggs for the female giant salamander extracted out in advance;

(6) fertilized eggs are totally submerged by the physiological saline of addition 0.7-0.9% after dripping off sperm five minutes, 15 points after submergence Clock completes fertilization;

(7) fertilized eggs are rinsed 5-7 times with clear water repeatedly after the completion of fertilization, to rinse out extra sperm completely;

(8) fertilized eggs are placed in hatchery after rinsing well, fertilized eggs are checked after 20 hours, if there is going mouldy, floating, become Color, shrivelled fertilized eggs will be cleared up in time;

(9) it can hatch completion at 30-35 days after clearing up.