CN105340791A - Artificial pseudaspius leptocephalus spawning inducing method - Google Patents
Artificial pseudaspius leptocephalus spawning inducing method Download PDFInfo
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- CN105340791A CN105340791A CN201510630875.4A CN201510630875A CN105340791A CN 105340791 A CN105340791 A CN 105340791A CN 201510630875 A CN201510630875 A CN 201510630875A CN 105340791 A CN105340791 A CN 105340791A
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- 238000000034 method Methods 0.000 title claims abstract description 30
- 241000063534 Pseudaspius leptocephalus Species 0.000 title abstract description 6
- 230000001939 inductive effect Effects 0.000 title abstract 2
- 241000251468 Actinopterygii Species 0.000 claims abstract description 53
- 239000003814 drug Substances 0.000 claims abstract description 26
- 229940079593 drug Drugs 0.000 claims abstract description 25
- 239000007924 injection Substances 0.000 claims abstract description 13
- 238000002347 injection Methods 0.000 claims abstract description 13
- 210000000006 pectoral fin Anatomy 0.000 claims abstract description 7
- 230000012447 hatching Effects 0.000 claims abstract description 5
- 230000008569 process Effects 0.000 claims abstract description 5
- 241001092500 Photinia x fraseri Species 0.000 claims description 34
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 30
- 210000000582 semen Anatomy 0.000 claims description 13
- 102000002322 Egg Proteins Human genes 0.000 claims description 12
- 108010000912 Egg Proteins Proteins 0.000 claims description 12
- 230000037396 body weight Effects 0.000 claims description 12
- 210000004681 ovum Anatomy 0.000 claims description 12
- 210000001015 abdomen Anatomy 0.000 claims description 11
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 10
- 235000013601 eggs Nutrition 0.000 claims description 10
- 239000008103 glucose Substances 0.000 claims description 10
- 239000003488 releasing hormone Substances 0.000 claims description 10
- 238000009395 breeding Methods 0.000 claims description 8
- 230000001488 breeding effect Effects 0.000 claims description 8
- 230000002196 ecbolic effect Effects 0.000 claims description 8
- 239000000243 solution Substances 0.000 claims description 8
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 6
- 210000003746 feather Anatomy 0.000 claims description 6
- FPAFDBFIGPHWGO-UHFFFAOYSA-N dioxosilane;oxomagnesium;hydrate Chemical compound O.[Mg]=O.[Mg]=O.[Mg]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O FPAFDBFIGPHWGO-UHFFFAOYSA-N 0.000 claims description 5
- 238000003756 stirring Methods 0.000 claims description 5
- 238000011534 incubation Methods 0.000 claims description 4
- 206010002091 Anaesthesia Diseases 0.000 claims description 3
- 230000037005 anaesthesia Effects 0.000 claims description 3
- 239000008239 natural water Substances 0.000 claims description 2
- 239000002349 well water Substances 0.000 claims description 2
- 235000020681 well water Nutrition 0.000 claims description 2
- 230000004720 fertilization Effects 0.000 abstract description 3
- 230000035800 maturation Effects 0.000 abstract description 3
- 238000009360 aquaculture Methods 0.000 abstract description 2
- 244000144974 aquaculture Species 0.000 abstract description 2
- 230000001360 synchronised effect Effects 0.000 abstract description 2
- 230000006698 induction Effects 0.000 abstract 6
- 230000017448 oviposition Effects 0.000 description 5
- 239000002504 physiological saline solution Substances 0.000 description 4
- 210000000436 anus Anatomy 0.000 description 2
- 238000007865 diluting Methods 0.000 description 2
- 230000032696 parturition Effects 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 241000272525 Anas platyrhynchos Species 0.000 description 1
- 241000238424 Crustacea Species 0.000 description 1
- 241000252210 Cyprinidae Species 0.000 description 1
- 241000252206 Cypriniformes Species 0.000 description 1
- 241001282110 Pagrus major Species 0.000 description 1
- 208000034189 Sclerosis Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 210000002149 gonad Anatomy 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 244000062804 prey Species 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- 230000035899 viability Effects 0.000 description 1
- 238000003911 water pollution Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The present invention provides an artificial pseudaspius leptocephalus spawning inducing method, and belongs to the technical field of aquaculture. The method aims to solve the technical problems of scattered laying times, a long laying period, asynchronous female and male parent fish maturation and a low hatching rate in the conventional wild pseudaspius leptocephalus natural laying process. The method comprises: selecting parents; injecting a medicine into a pectoral fin base portion of a female parent fish and injecting a spawning induction drug into a pectoral base portion of a male parent fish; performing artificial fertilization after performing drug injection once; and completing artificial spawning induction of pseudaspius leptocephalus. The method provided by the present invention enables maturation of female and male parent fishes to be synchronous and enables the laying time to be concentrated, the laying period is shortened by 10-15 days, the fertilization rate reaches 75-80%, and the hatching rate reaches 60-70%; and artificial spawning induction is performed on the parent fishes, the spawning induction success rate of the male parent fish reaches 95%, and the spawning induction success rate of the female parent fish reaches 75-80%. The method provided by the present invention is used for artificial spawning induction of pseudaspius leptocephalus .
Description
Technical field
The invention belongs to technical field of aquaculture, be specifically related to a kind of induced spawning method intending red tip fish.
Background technology
Intend red tip fish
pseudaspiusleptocephalus, belong to Cypriniformes, Cyprinidae, Leuciscinae, intend red tip fish and belong to, be one of Heilongjiang Province name fish " three spend five sieve ", be commonly called as by local common people: duck sieve, red tail mortise pin, sharp mouth etc.Intend red tip fish to be distributed in Upper Reaches of The Nenjiang River, china, dry, the tributary in Heilungkiang and attached lake thereof, like inhabiting the lower running water of water temperature, the stage of fattening does not enter lake, only preys in gulf, river; Winter is still movable, continues to ingest.Major Foods is based on small fishes or crustacean; Growth suitable temperature range is 0 DEG C ~ 30 DEG C, optimum growth temperature 22 DEG C ~ 26 DEG C.Intend red tip fish head point body narrow, back is slightly black, health silvery white, and meat silvery white is delicate, delicious flavour, and cultivation temperature range is wide, is the important Quality and economy fish in Heilongjiang Province, is expected to become new famous-brand and high-quality Special Breed kind.
At occurring in nature, intend red tip fish exist raun lay eggs do not concentrate, the duration longer (oviposition period is about about one month), the problem such as a male and female parent population body maturation asynchronous (milter gonad maturity is more late than raun).In recent years, due to artificial transition fish for, the reason such as water pollution and spawning ground destruction causes the natural resources amount intending red tip fish sharply to decline.Therefore, carry out the research of the artificial propagation techniques intending red tip fish, for intend red tip fish wild resource recover, protection and new varieties have great importance.
Summary of the invention
The object of this invention is to provide and a kind of intend red tip fish induced spawning method, quasi-solution certainly occurs that egg-laying time is not concentrated, oviposition period is long in existing wild plan red tip fish natural spawning process, the ripe asynchronous and technical problem that incubation rate is low of male and female parent population.
The object of the invention is to be achieved through the following technical solutions:
Plan of the present invention red tip fish induced spawning method, described method step is as follows:
Step one, select body weight be greater than 0.75kg and more than 4 ages raun as raun parent; Select body weight be greater than 0.5kg and more than 3 ages milter as milter parent;
Step 2, in annual May, treat that water temperature is increased to 18 DEG C, after fishing for the raun parent and milter parent anesthesia that step one selects, drug injection is carried out at raun parent pectoral fin base portion, injection depth is 4 ~ 6mm, and raun parent injectable drug ratio is: inject 1 milliliter of medical glucose containing 10 No. 3, μ g luteotropin releasing hormone d-ala analogs, 1000IU human chorionic gonadtropin and 4mg DOM or mixed liquor of normal saline by per kilogram of body weight;
After step 3, raun parental drug inject 12 hours, water temperature is higher than under the condition of 18 DEG C, oxytocic drug injection is carried out at milter parent pectoral fin base portion, injection depth is 4 ~ 6mm, and milter parent oxytocic drug injectable drug ratio is: inject 1 milliliter of medical glucose containing 5 No. 3, μ g luteotropin releasing hormone d-ala analogs, 500IU human chorionic gonadtropin and 2mg DOM or mixed liquor of normal saline by per kilogram of body weight;
After step 4, milter parental drug inject 12 hours, under the condition of water temperature higher than 19 DEG C, fish for 6 ~ 8 tail milter parent collecting semens, every tail milter parent seminal fluid is positioned over separately in 4 DEG C of refrigerators and preserves; Then get raun parent and carry out ovum collection, immediately the seminal fluid of 6 ~ 8 tail milters be collected before is squeezed in ovum surface with the amount of every tail 0.3ml after often gathering the ovum of a tail raun parent, 25 ~ 35s is stirred with feather, then 180 ~ 220ml clear water is added,, rapid stirring 1 ~ 2min even with feather again, stirs fertilized egg, after unsticking 8 ~ 10min with putting into talcum powder solution by fertilized egg, put into hatching bottle to hatch, namely complete the artificial induced spawning process intending red tip fish.
The present invention relative to the beneficial effect of prior art is: plan of the present invention red tip fish induced spawning method makes that female milter parent is ripe synchronous, egg-laying time is concentrated, oviposition period shortens 10 ~ 15 days, and fertilization rate is up to 75 ~ 80%, incubation rate is up to 60% ~ 70%; Artificial induced spawning intends red tip fish parent fish, and the success rate of hastening parturition of milter parent reaches 95%, and the success rate of hastening parturition of raun parent reaches 75% ~ 80%.
Embodiment
Below technical scheme of the present invention is further described, but is not limited thereto, everyly technical solution of the present invention is modified or equivalent to replace, and do not depart from the spirit and scope of technical solution of the present invention, all should be encompassed in protection scope of the present invention.
Embodiment one: what present embodiment was recorded is intend red tip fish induced spawning method, and described method step is as follows:
Step one, select (build is good, energy is strong and without wound) body weight be greater than 0.75kg and more than 4 ages raun as raun parent; Select (build is good, energy strong and without wound) body weight be greater than 0.5kg and more than 3 ages milter as milter parent;
Step 2, in annual May, treat that water temperature is increased to 18 DEG C, after fishing for the raun parent and milter parent anesthesia that step one selects, drug injection is carried out at raun parent pectoral fin base portion, injection depth is 4 ~ 6mm, and raun parent injectable drug ratio is: inject 1 milliliter of medical glucose containing 10 No. 3, μ g luteotropin releasing hormone d-ala analogs, 1000IU human chorionic gonadtropin and 4mg DOM or mixed liquor of normal saline by per kilogram of body weight; The female parent population of 97% can be induced to discharge ovum in 24 hours;
That is: No. 3, described luteotropin releasing hormone d-ala analog, human chorionic gonadtropin and DOM all carry out mixed diluting with medical glucose or physiological saline, and concentration is containing 10 No. 3, μ g luteotropin releasing hormone d-ala analogs, 1000IU human chorionic gonadtropin and 4mg DOM in every milliliter of medical glucose or physiological saline.
After step 3, raun parental drug inject 12 hours, water temperature is higher than under the condition of 18 DEG C, oxytocic drug injection is carried out at milter parent pectoral fin base portion, injection depth is 4 ~ 6mm, and milter parent oxytocic drug injectable drug ratio is: inject 1 milliliter of medical glucose containing 5 No. 3, μ g luteotropin releasing hormone d-ala analogs, 500IU human chorionic gonadtropin and 2mg DOM or mixed liquor of normal saline by per kilogram of body weight; Can induce in the male parent population 24 of 95% and discharge seminal fluid;
That is: No. 3, described luteotropin releasing hormone d-ala analog, human chorionic gonadtropin and DOM all carry out mixed diluting with medical glucose or physiological saline, and concentration is containing 5 No. 3, μ g luteotropin releasing hormone d-ala analogs, 500IU human chorionic gonadtropin and 2mg DOM in every milliliter of medical glucose or physiological saline.
After step 4, milter parental drug inject 12 hours, under the condition of water temperature higher than 19 DEG C, fish for 6 ~ 8 tail milter parent collecting semens, every tail milter parent seminal fluid is positioned over separately in 4 DEG C of refrigerators and preserves; Then get raun parent and carry out ovum collection, immediately the seminal fluid of 6 ~ 8 tail milters be collected before is squeezed in ovum surface with the amount of every tail 0.3ml after often gathering the ovum of a tail raun parent, 25 ~ 35s is stirred with feather, then 180 ~ 220ml clear water is added,, rapid stirring 1 ~ 2min even with feather again, fertilized egg is stirred with putting into talcum powder solution (matched proportion density of talcum powder solution talcum powder and water is 20g/L) by fertilized egg, after unsticking (professional term) 8 ~ 10min, put into hatching bottle to hatch, namely complete the artificial induced spawning process intending red tip fish.
Embodiment two: the red tip fish of the plan described in embodiment one induced spawning method, in step 4, fishes for raun parent successively, is dried by the water around belly and cloacal aperture with dry towel, is then clamp-oned by fish-egg and lays eggs in basin; After avoiding part ovum to contact water, egg membrane sclerosis cannot be fertilized.
Embodiment three: the red tip fish of the plan described in embodiment one induced spawning method, in step 4, get after 6 ~ 8 tail milter parents fish for, with dry towel, the water around belly and cloacal aperture is dried, every tail milter parent seminal fluid is extruded in small beaker by light pressure belly, is positioned over 4 DEG C of low temperature, lucifuge place preserves separately.Ensure sperm viability.
Embodiment four: the red tip fish of the plan described in embodiment one induced spawning method, before step one, also comprise one intend red tip fish parent fish incubation step, namely in annual late May, the red tip fish of sexually matured plan is fished for individual in natural water, be positioned in indoor water mud sump, breeding water is the limpid water body of natural well water, rivers or reservoir, and fishpond drainage is unobstructed, the depth of water is at more than 0.5m, and breeding density is 4 ~ 5kg/m
2, it is 3:1 that male and female intend red tip fish ratio, throws something and feeds with the crucian living bait of body length at 4.5cm ~ 5.5cm.The survival rate of parent population individuality is made to bring up to 80%.
Embodiment five: the red tip fish of the plan described in embodiment four induced spawning method, the depth of water is at more than 0.6m, and breeding density is 5kg/m
2.The survival rate of parent population individuality is made to bring up to 80%.
Raun parent and milter parent selecting and differentiating:
At non-breeding season, slightly swell between milter anus and gonopore, raun is not swelled; Breeding season: raun belly is soft, expand, body is wide, and anus and gonopore are slightly red outstanding not obvious, extrudes belly find a small amount of ovum grain when not injecting oxytocic drug; Milter belly is less, and body is wide narrower, and stroke belly with hand comparatively hard, gonopore is given prominence to, and extrudes belly can find seminal fluid when not injecting oxytocic drug.
Claims (5)
1. intend a red tip fish induced spawning method, it is characterized in that: described method step is as follows:
Step one, select body weight be greater than 0.75kg and more than 4 ages raun as raun parent; Select body weight be greater than 0.5kg and more than 3 ages milter as milter parent;
Step 2, in annual May, treat that water temperature is increased to 18 DEG C, after fishing for the raun parent and milter parent anesthesia that step one selects, drug injection is carried out at raun parent pectoral fin base portion, injection depth is 4 ~ 6mm, and raun parent injectable drug ratio is: inject 1 milliliter of medical glucose containing 10 No. 3, μ g luteotropin releasing hormone d-ala analogs, 1000IU human chorionic gonadtropin and 4mg DOM or mixed liquor of normal saline by per kilogram of body weight;
After step 3, raun parental drug inject 12 hours, water temperature is higher than under the condition of 18 DEG C, oxytocic drug injection is carried out at milter parent pectoral fin base portion, injection depth is 4 ~ 6mm, and milter parent oxytocic drug injectable drug ratio is: inject 1 milliliter of medical glucose containing 5 No. 3, μ g luteotropin releasing hormone d-ala analogs, 500IU human chorionic gonadtropin and 2mg DOM or mixed liquor of normal saline by per kilogram of body weight;
After step 4, milter parental drug inject 12 hours, under the condition of water temperature higher than 19 DEG C, fish for 6 ~ 8 tail milter parent collecting semens, every tail milter parent seminal fluid is positioned over separately in 4 DEG C of refrigerators and preserves; Then get raun parent and carry out ovum collection, immediately the seminal fluid of 6 ~ 8 tail milters be collected before is squeezed in ovum surface with the amount of every tail 0.3ml after often gathering the ovum of a tail raun parent, 25 ~ 35s is stirred with feather, then 180 ~ 220ml clear water is added,, rapid stirring 1 ~ 2min even with feather again, stirs fertilized egg, after unsticking 8 ~ 10min with putting into talcum powder solution by fertilized egg, put into hatching bottle to hatch, namely complete the artificial induced spawning process intending red tip fish.
2. plan according to claim 1 red tip fish induced spawning method, is characterized in that: in step 4, raun parent is fished for successively, is dried by the water around belly and cloacal aperture with dry towel, is then clamp-oned by fish-egg and lays eggs in basin.
3. plan according to claim 1 red tip fish induced spawning method, it is characterized in that: in step 4, get after 6 ~ 8 tail milter parents fish for, with dry towel, the water around belly and cloacal aperture is dried, every tail milter parent seminal fluid is extruded in small beaker by light pressure belly, is positioned over 4 DEG C of low temperature, lucifuge place preserves separately.
4. plan according to claim 1 red tip fish induced spawning method, it is characterized in that: before step one, also comprise one intend red tip fish parent fish incubation step, namely in annual late May, the red tip fish of sexually matured plan is fished for individual in natural water, be positioned in indoor water mud sump, breeding water is the limpid water body of natural well water, rivers or reservoir, and fishpond drainage is unobstructed, the depth of water is at more than 0.5m, and breeding density is 4 ~ 5kg/m
2, it is 3:1 that male and female intend red tip fish ratio, throws something and feeds with the crucian living bait of body length at 4.5cm ~ 5.5cm.
5. plan according to claim 4 red tip fish induced spawning method, is characterized in that: the depth of water is at more than 0.6m, and breeding density is 5kg/m
2.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510630875.4A CN105340791A (en) | 2015-09-29 | 2015-09-29 | Artificial pseudaspius leptocephalus spawning inducing method |
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| CN201510630875.4A CN105340791A (en) | 2015-09-29 | 2015-09-29 | Artificial pseudaspius leptocephalus spawning inducing method |
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| CN105340791A true CN105340791A (en) | 2016-02-24 |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107018935A (en) * | 2017-05-23 | 2017-08-08 | 中国水产科学研究院黑龙江水产研究所 | A kind of wild makeup of chum salmon seedling is put |
| CN108186157A (en) * | 2018-02-09 | 2018-06-22 | 武汉中科瑞华生态科技股份有限公司 | A kind of artificial insemination method of C. guichenoti |
| CN108283152A (en) * | 2018-03-28 | 2018-07-17 | 武汉中科瑞华生态科技股份有限公司 | A kind of long filament schizothoracin artificial fecundation method |
-
2015
- 2015-09-29 CN CN201510630875.4A patent/CN105340791A/en active Pending
Non-Patent Citations (2)
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| 于信勇 等: "拟赤梢鱼人工繁殖的初步研究", 《中国水产》 * |
| 杨景峰 等: "半滑舌鳎人工催产技术研究", 《内蒙古民族大学学报（自然科学版）》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107018935A (en) * | 2017-05-23 | 2017-08-08 | 中国水产科学研究院黑龙江水产研究所 | A kind of wild makeup of chum salmon seedling is put |
| CN107018935B (en) * | 2017-05-23 | 2023-07-21 | 中国水产科学研究院黑龙江水产研究所 | Wild device of marijuana fry |
| CN108186157A (en) * | 2018-02-09 | 2018-06-22 | 武汉中科瑞华生态科技股份有限公司 | A kind of artificial insemination method of C. guichenoti |
| CN108283152A (en) * | 2018-03-28 | 2018-07-17 | 武汉中科瑞华生态科技股份有限公司 | A kind of long filament schizothoracin artificial fecundation method |
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Application publication date: 20160224 |
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