CN103190368A - Breeding method for distant hybridization between erythroculter ilishaeformis and ancherythropterus nigrocauda - Google Patents
Breeding method for distant hybridization between erythroculter ilishaeformis and ancherythropterus nigrocauda Download PDFInfo
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- CN103190368A CN103190368A CN2013101192875A CN201310119287A CN103190368A CN 103190368 A CN103190368 A CN 103190368A CN 2013101192875 A CN2013101192875 A CN 2013101192875A CN 201310119287 A CN201310119287 A CN 201310119287A CN 103190368 A CN103190368 A CN 103190368A
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Abstract
The invention discloses a breeding method for distant hybridization between erythroculter ilishaeformis and ancherythropterus nigrocauda. The method includes the following steps: 1) selecting selectively-bred erythroculter ilishaeformis and selectively-bred ancherythropterus nigrocauda as parents; 2) conducting parents strengthening breeding including erythroculter ilishaeformis strengthening breeding and ancherythropterus nigrocauda strengthening breeding; 3) conducting artificial spawning induction: respectively injecting spawning induction medicine hypophysis cerebri or dopamine antagonist DOM, lutein releasing hormone analogue and chorionic gonadotropin which are used singly or in mixing mode on the two parents; 4) conducting artificial semen and spawn collection and insemination including dry method insemination and semi-dry method insemination; and 5) conducting artificial incubation. Hybridized culter 'vanguard 1' obtained by distant hybridization has the advantages of good body type and large size of the erythroculter ilishaeformis, also has the advantages of low breeding cost, mild temper, easiness in catching and easiness in live marketing of the ancherythropterus nigrocauda and further has excellent hybridization performances like high growing speed.
Description
Technical field
The present invention relates to distant hybrid breeding method between the nearly red Culter of a kind of erythroculter ilishaeformis and black tail.
Background technology
Culter veriety meat is tender delicious, is the famous and precious aquatic products that the consumer likes food.In the Culter veriety of promote culturing, erythroculter ilishaeformis maximum individual naturally greatly, build market receptance height, but have the protein requirement amount height of feed, the aquaculture cost height, weak point such as is difficult for fishing at edgy difficult transportation; The nearly red Culter aquaculture cost of black tail is low, the growth fast, disposition is docile, easily fish for, easily live fish transportation, but has maximum weak points such as individuality is less naturally, build is wideer.
Summary of the invention
The technical problem to be solved in the present invention provides a kind of weak point that overcomes erythroculter ilishaeformis and the nearly red Culter of black tail, and absorbs distant hybrid breeding method between the erythroculter ilishaeformis of the two advantage and the nearly red Culter of black tail.
For addressing the above problem, the present invention adopts following technical scheme: distant hybrid breeding method between the nearly red Culter of a kind of erythroculter ilishaeformis and black tail may further comprise the steps:
1), select winter and spring, select maturation, health and do the parent through the erythroculter ilishaeformis of continuous four generations seed selection with through the nearly red Culter of the black tail of continuous four generations seed selection;
2), parent's reinforced cultivating: comprise the reinforced cultivating of erythroculter ilishaeformis parent population and the reinforced cultivating of the nearly red Culter parent population of black tail;
3), artificial induced spawning: select June to July, respectively two kinds of parents are injected oxytocic drug carp hypophysis or dopamine antagonist DOM, lutern releasing hormone analog, human chorionic gonadtropin, use separately or mix and use;
4), artificial semen collection ovum insemination: the parent behind the injection oxytocic drug divides and supports in spawning inducing pool, and erythroculter ilishaeformis raun, the insemination of the artificial semen collection ovum of the nearly red Culter milter of black tail dry method or semi-dry process insemination are selected in hauling fishing respectively when treating that parent population oestruses; Female, male parent population ratio is 1:1 ~ 2;
5), artificial incubation: the exomixis ovum is gone into the flushing type incubation tank or hatch in cylinder, the circular hatching channal to hatch, and hatching density is 50 ~ 600,000/m
3Water body, the hatching current are with fish-egg degree of being deposited as not; When " waist point " when occurring, shifts out incubation equipment, to the pond, carry out the summer flower nurture of fish fingerlings.
As preferably, the described the 2nd) step in,
The reinforced cultivating of described erythroculter ilishaeformis parent population: press female-male proportion 1:1 ~ 2, stocking rate is 50 ~ 60 tail/mus, and the living fry of throwing something and feeding, ice fresh fish and special compound feed are as parent population bait; From beginning by the end of April, change water weekly 1 time, irregularly bath simultaneously, the gonad development of promotion erythroculter ilishaeformis parent population;
The reinforced cultivating of the nearly red Culter parent population of described black tail: female-male proportion 1:3 ~ 4, stocking rate are 200 ~ 300 tail/mus, and the crude protein content of throwing something and feeding is the manufactured feed more than 32%; During early spring, the disposable high water level that adds reduces in the pond and changes water, reduces accumulated temperature; The male and female parent population divides the pond to cultivate when approaching the breeding temperature, thereby slows down the gonad maturity time.
As preferably, the described the 3rd) step in,
The dosage of hastening parturition of erythroculter ilishaeformis raun is every kilogram of parent population carp hypophysis 2mg ~ 3mg, human chorionic gonadtropin 800IU ~ 1200IU, lutern releasing hormone analog 2 μ g ~ 3.5 μ g or dopamine antagonist DOM 3mg ~ 4mg; The use that reduces by half of the above dosage of milter.
The dosage of hastening parturition of the nearly red Culter raun of black tail is every kilogram of parent population carp hypophysis 1.5mg ~ 2mg, human chorionic gonadtropin 600IU ~ 1200IU, lutern releasing hormone analog 2 μ g ~ 2.5 μ g or dopamine antagonist DOM 2mg ~ 3mg; The use that reduces by half of the above dosage of milter.
As preferably, the described the 4th) step in,
Dry method insemination: the female fish egg of erythroculter ilishaeformis is clamp-oned in the vessel of drying, clamp-oned the nearly red Culter milter seminal fluid of black tail simultaneously, forbid bringing into moisture; Evenly stir with dried feather, add a small amount of 0.65% physiological saline and stir gently, add a large amount of clear water again and stir evenly.
Semi-dry process insemination: the female fish egg of erythroculter ilishaeformis is clamp-oned in the vessel that fill a small amount of 0.65% physiological saline, clamp-oned the nearly red Culter milter seminal fluid of black tail simultaneously, and evenly stir simultaneously with dried feather, add a large amount of clear water again and stir evenly.
The invention has the beneficial effects as follows: the present invention is to be female parent with the Danjiangkou Reservoir erythroculter ilishaeformis through seed selection, with be male parent through the nearly red Culter of the Upper Yangtze River black tail of seed selection, good, the individual big advantage of the existing erythroculter ilishaeformis build of hybridization Culter " pioneer No. 1 " of far hybridizing acquisition through edge, have again that the nearly red Culter aquaculture cost of black tail is low, disposition is docile, easily fish for, the easy advantage of live fish listing, also have good hybridization proterties such as fast growth.
Description of drawings
Fig. 1 is the schematic diagram of distant hybrid breeding method between erythroculter ilishaeformis of the present invention and the nearly red Culter of black tail.
Embodiment
Consult shown in Figure 1, distant hybrid breeding method between the nearly red Culter of a kind of erythroculter ilishaeformis and black tail, determine with the Danjiangkou Reservoir erythroculter ilishaeformis through seed selection to be female parent, with be male parent through the nearly red Culter of the Upper Yangtze River black tail of seed selection, by the first-filial generation that distant hybridization cultivates, cultivate hybridization Culter " pioneer No. 1 " with this hybrid combination.China's geographical sign kind-Danjiangkou Reservoir erythroculter ilishaeformis has that disposition is docile relatively, mature egg is characteristics such as drift gamogenetic egg, cultures proterties and is better than Taihu Lake erythroculter ilishaeformis and other waters erythroculter ilishaeformis of this province.Be female parent with the Danjiangkou Reservoir erythroculter ilishaeformis, nearly red Culter is male parent with the Upper Yangtze River black tail, and the hybridization Culter of cultivation " pioneer No. 1 " can not natural propagation in the hydrostatic water body.
Determine that with the Danjiangkou Reservoir erythroculter ilishaeformis be the maternal hybridization Culter " pioneer No. 1 " that cultivates, good, the individual big advantage of existing erythroculter ilishaeformis build, have again that the nearly red Culter aquaculture cost of black tail is low, disposition is docile, easily fish for, the easy advantage of live fish listing, also have than good hybridization proterties such as parents fast growths.
This method may further comprise the steps:
(1) selects winter and spring, select maturation, health and through the Hubei Province of continuous four generations seed selection Danjiangkou Reservoir erythroculter ilishaeformis with flow the nearly red Culter of black tail through the Changjiang river, Sichuan Province of the continuous four generations seed selection Heavenly Stems and Earthly Branches and do the parent.
(2) parent's reinforced cultivating
The reinforced cultivating of a, erythroculter ilishaeformis parent population: press female-male proportion 1:1 ~ 2, stocking rate is 50 ~ 60 tail/mus, and the living fry of throwing something and feeding, ice fresh fish and special compound feed are as parent population bait; From beginning by the end of April, change water weekly 1 time, irregularly bath simultaneously, the gonad development of promotion erythroculter ilishaeformis parent population.
The reinforced cultivating of b, the nearly red Culter parent population of black tail: female-male proportion 1:3 ~ 4, stocking rate are 200 ~ 300 tail/mus, and the crude protein content of throwing something and feeding is the manufactured feed more than 32%; During early spring, the disposable high water level that adds reduces in the pond and changes water, reduces accumulated temperature; The male and female parent population divides the pond to cultivate when approaching the breeding temperature, thereby slows down the gonad maturity time.
(3) artificial induced spawning
Select June to July, respectively two kinds of parents are injected oxytocic drug carp hypophysis (PG) or dopamine antagonist DOM (DOM), lutern releasing hormone analog (LRH-A), human chorionic gonadtropin (HCG), use separately or mix and use.
Wherein, the dosage of hastening parturition of erythroculter ilishaeformis raun is every kilogram of parent population PG2mg ~ 3mg, HCG800IU ~ 1200IU, LRH-A
22 μ g ~ 3.5 μ g or DOM3mg ~ 4mg, the dosage of hastening parturition of the nearly red Culter raun of black tail are every kilogram of parent population PG1.5mg ~ 2mg, HCG600IU ~ 1200IU, LRH-A
22 μ g ~ 2.5 μ g or DOM2mg ~ 3mg; The use that reduces by half of the above dosage of milter.
(4) artificial semen collection ovum insemination
The parent of injection behind the oxytocic drug divides and supports in spawning inducing pool, and erythroculter ilishaeformis raun, the insemination of the artificial semen collection ovum of the nearly red Culter milter of black tail dry method or semi-dry process insemination are selected in hauling fishing respectively when treating that parent population oestruses; Female, male parent population ratio is 1:1 ~ 2.
Wherein, dry method insemination: the female fish egg of erythroculter ilishaeformis is clamp-oned in the vessel of drying, clamp-oned the nearly red Culter milter seminal fluid of black tail simultaneously, forbid bringing into moisture; Evenly stir with dried feather, add a small amount of 0.65% physiological saline and stir gently, add a large amount of clear water again and stir evenly.
Semi-dry process insemination: the female fish egg of erythroculter ilishaeformis is clamp-oned in the vessel that fill a small amount of 0.65% physiological saline, clamp-oned the nearly red Culter milter seminal fluid of black tail simultaneously, and evenly stir simultaneously with dried feather, add a large amount of clear water again and stir evenly.
(5) artificial incubation
The exomixis ovum is gone into the flushing type incubation tank or hatch in cylinder, the circular hatching channal to hatch, and hatching density is 50 ~ 600,000/m
3Water body, the hatching current are with fish-egg degree of being deposited as not; When " waist point " when occurring, shifts out incubation equipment, to the pond, carry out the summer flower nurture of fish fingerlings.
Wherein, a, parent select
We are in the seed selection and breeding work of erythroculter ilishaeformis, find by culturing with investigation, this fish maximum individual naturally greatly, build market receptance height, fish brood amount be big, but the protein requirement amount height (crude protein content is more than 40%) that has feed, the aquaculture cost height, weak point such as is difficult for fishing at edgy difficult transportation; Find that simultaneously China's geographical sign kind---Danjiangkou Reservoir erythroculter ilishaeformis has that disposition is docile relatively, mature egg is characteristics such as drift gamogenetic egg, cultures proterties and is better than Taihu Lake erythroculter ilishaeformis and other waters erythroculter ilishaeformis of this province.
We introduce the nearly red Culter of wild black tail from the Heavenly Stems and Earthly Branches stream of Upper Yangtze River, carried out the research and development of artificial domestication, breeding and seed selection and serial application; In the research and extension process, find this fish culture cost low (crude protein content of feed is more than 32%), disposition docility, easily fish for, easily live fish transportation, but have maximum weak points such as individuality is less naturally, build is wideer.
According to the advantage of Danjiangkou Reservoir erythroculter ilishaeformis and the nearly red Culter of black tail, selecting the two is the parent, has carried out these two the kind distant hybridization breeding tests that do not belong to together.
B, parent's seed selection
In academy of aquatic sciences, Wuhan City and Wuhan pioneer Aqua Sciences Inc. base, nineteen ninety-five, 1999 obtain erythroculter ilishaeformis selfing F respectively
1With the nearly red Culter selfing F of black tail
1After this, according to the fine-variety breeding technology path, adopt colony's selection and fine-variety breeding technology by for strengthening screening, pick out the individuality that growth is fast, build is good and be used as the parent; Acquisition erythroculter ilishaeformis F in 3 age in winter in 2007
4Generation and the nearly red Culter F of 2 black tails in age in winter
4For parental population.
C, crossbreeding
2007, carry out erythroculter ilishaeformis F
4The nearly red Culter F of generation and black tail
4For crossbreeding test and correlative study, select erythroculter ilishaeformis (♀) F
4The nearly red Culter of generation and black tail (♂) F
4Cultivate hybridization Culter " pioneer No. 1 " for hybrid combination, its heterosis, hybrid vigor shows as:
1. fast growth.Adopt crude protein content be 32% manufactured feed with pool cultivated, it is fast 23.6%～29.6% to grow than the nearly red Culter of black tail, grows fast 100.3%～172.4% than erythroculter ilishaeformis; Adopt tripartite all suitable feed monoculture, it is fast 22.8%～23.3% to grow than the nearly red Culter of black tail, grows fast 24.1%～35.4% than erythroculter ilishaeformis.
2. feed cost is low, reduces by 50.0 %～52.4% than erythroculter ilishaeformis.
3. build is good, is easily admitted by market.
4. disposition docility is easily fished for, easily live fish listing.
The good characteristic of hybridization Culter " pioneer No. 1 ":
(1) hybridization Culter " pioneer No. 1 " fast growth.Adopt crude protein content be 32% manufactured feed with pool cultivated, it is fast 23.6%～29.6% to grow than the nearly red Culter of black tail, grows fast 100.3%～172.4% than erythroculter ilishaeformis.
(2) feed cost of hybridization Culter " pioneer No. 1 " reduces by 50.0 %～52.4% than erythroculter ilishaeformis.
(3) it is higher that hybridization Culter " pioneer No. 1 " works the rate of catching; The disposition docility is easily fished for, easily live fish listing; Build is good, between parents, is easily admitted by market.
The present invention adopts the Danjiangkou Reservoir erythroculter ilishaeformis through seed selection to be female parent, with be male parent through the nearly red Culter of the Upper Yangtze River black tail of seed selection, the hybridization Culter of cultivation " pioneer No. 1 " has that build is good, fast growth, individual big, aquaculture cost is low, disposition is docile, easily fish for, the easy advantage of live fish listing; Be female parent as adopting other waters erythroculter ilishaeformis through seed selection, with be male parent through the nearly red Culter of the Upper Yangtze River black tail of seed selection, the hybridization Culter that cultivates also can have advantages such as build is good, fast growth, individuality is big, aquaculture cost is low, but energy natural propagation in the hydrostatic water body easily causes gene contamination.
The above only is the specific embodiment of the present invention, but protection scope of the present invention is not limited thereto, and any variation or replacement of expecting without creative work all should be encompassed within protection scope of the present invention.Therefore, protection scope of the present invention should be as the criterion with the protection domain that claims were limited.
Claims (4)
1. distant hybrid breeding method between an erythroculter ilishaeformis and the nearly red Culter of black tail is characterized in that: may further comprise the steps:
1), select winter and spring, select maturation, health and do the parent through the erythroculter ilishaeformis of seed selection with through the nearly red Culter of the black tail of seed selection;
2), parent's reinforced cultivating: comprise the reinforced cultivating of erythroculter ilishaeformis parent population and the reinforced cultivating of the nearly red Culter parent population of black tail;
3), artificial induced spawning: select June to July, respectively two kinds of parents are injected oxytocic drug carp hypophysis or dopamine antagonist DOM, lutern releasing hormone analog, human chorionic gonadtropin, use separately or mix and use;
4), artificial semen collection ovum insemination: the parent behind the injection oxytocic drug divides and supports in spawning inducing pool, and erythroculter ilishaeformis raun, the insemination of the artificial semen collection ovum of the nearly red Culter milter of black tail dry method or semi-dry process insemination are selected in hauling fishing respectively when treating that parent population oestruses; Female, male parent population ratio is 1:1 ~ 2;
5), artificial incubation: the exomixis ovum is gone into the flushing type incubation tank or hatch in cylinder, the circular hatching channal to hatch, and hatching density is 50 ~ 600,000/m
3Water body, the hatching current are with fish-egg degree of being deposited as not; When " waist point " when occurring, shifts out incubation equipment, to the pond, carry out the summer flower nurture of fish fingerlings.
2. distant hybrid breeding method between the nearly red Culter of erythroculter ilishaeformis according to claim 1 and black tail is characterized in that: the described the 2nd) in the step,
The reinforced cultivating of described erythroculter ilishaeformis parent population: press female-male proportion 1:1 ~ 2, stocking rate is 50 ~ 60 tail/mus, and the living fry of throwing something and feeding, ice fresh fish and special compound feed are as parent population bait; From beginning by the end of April, change water weekly 1 time, irregularly bath simultaneously, the gonad development of promotion erythroculter ilishaeformis parent population;
The reinforced cultivating of the nearly red Culter parent population of described black tail: female-male proportion 1:3 ~ 4, stocking rate are 200 ~ 300 tail/mus, and the crude protein content of throwing something and feeding is the manufactured feed more than 32%; During early spring, the disposable high water level that adds reduces in the pond and changes water, reduces accumulated temperature; The male and female parent population divides the pond to cultivate when approaching the breeding temperature, thereby slows down the gonad maturity time.
3. distant hybrid breeding method between the nearly red Culter of erythroculter ilishaeformis according to claim 1 and black tail, it is characterized in that: the described the 3rd) in the step, the dosage of hastening parturition of erythroculter ilishaeformis raun is every kilogram of parent population carp hypophysis 2mg ~ 3mg, human chorionic gonadtropin 800IU ~ 1200IU, lutern releasing hormone analog 2 μ g ~ 3.5 μ g or dopamine antagonist DOM 3mg ~ 4mg; The use that reduces by half of the above dosage of milter;
The dosage of hastening parturition of the nearly red Culter raun of black tail is every kilogram of parent population carp hypophysis 1.5mg ~ 2mg, human chorionic gonadtropin 600IU ~ 1200IU, lutern releasing hormone analog 2 μ g ~ 2.5 μ g or dopamine antagonist DOM 2mg ~ 3mg; The use that reduces by half of the above dosage of milter.
4. distant hybrid breeding method between the nearly red Culter of erythroculter ilishaeformis according to claim 1 and black tail is characterized in that: the described the 4th) in the step,
Dry method insemination: the female fish egg of erythroculter ilishaeformis is clamp-oned in the vessel of drying, clamp-oned the nearly red Culter milter seminal fluid of black tail simultaneously, forbid bringing into moisture; Evenly stir with dried feather, add a small amount of 0.65% physiological saline and stir gently, add a large amount of clear water again and stir evenly;
Semi-dry process insemination: the female fish egg of erythroculter ilishaeformis is clamp-oned in the vessel that fill a small amount of 0.65% physiological saline, clamp-oned the nearly red Culter milter seminal fluid of black tail simultaneously, and evenly stir simultaneously with dried feather, add a large amount of clear water again and stir evenly.
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Cited By (5)
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| CN104938380A (en) * | 2015-06-01 | 2015-09-30 | 周天赐 | Artificial breeding method for ancherythroculter nigrocauda |
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| CN105475196A (en) * | 2015-12-18 | 2016-04-13 | 武汉先锋水产科技有限公司 | Distant hybridization method for megalobrama amblycephala and ancherythroculter nigrocauda |
| CN105684962A (en) * | 2015-11-17 | 2016-06-22 | 浙江省淡水水产研究所 | A culter alburnus and triangular bream crossbreed preparation method |
| CN113558044A (en) * | 2021-08-28 | 2021-10-29 | 上海海洋大学 | Erythroculter ilishaeformis sperm cryopreservation technology |
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| CN105684962A (en) * | 2015-11-17 | 2016-06-22 | 浙江省淡水水产研究所 | A culter alburnus and triangular bream crossbreed preparation method |
| CN105684962B (en) * | 2015-11-17 | 2018-05-11 | 浙江省淡水水产研究所 | One kind sticks up mouth Culter and triangular bream hybrid preparation method |
| CN105475196A (en) * | 2015-12-18 | 2016-04-13 | 武汉先锋水产科技有限公司 | Distant hybridization method for megalobrama amblycephala and ancherythroculter nigrocauda |
| CN113558044A (en) * | 2021-08-28 | 2021-10-29 | 上海海洋大学 | Erythroculter ilishaeformis sperm cryopreservation technology |
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