CN103875567A - Artificial spawning method for osteoglossum bicirrhosum - Google Patents
Artificial spawning method for osteoglossum bicirrhosum Download PDFInfo
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- CN103875567A CN103875567A CN201410105440.3A CN201410105440A CN103875567A CN 103875567 A CN103875567 A CN 103875567A CN 201410105440 A CN201410105440 A CN 201410105440A CN 103875567 A CN103875567 A CN 103875567A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention discloses an artificial spawning method for osteoglossum bicirrhosum. The method comprises the steps of the antenatal cultivation of parent fish of the osteoglossum bicirrhosum and the artificial spawning process of the osteoglossum bicirrhosum. According to the method, it is ensured that the osteoglossum bicirrhosum can obtain more germ cells, the hatchability of the germ cells and the survival rate of the fish fry are both high, breeding of the osteoglossum bicirrhosum in the northern China is achieved, and the high economic value is obtained.
Description
Technical field
The present invention relates to the induced spawning method of fish, particularly relate to the induced spawning method of the imperial fish of a kind of silver.
Background technology
The imperial fish of silver has another name called two palpus bone fishes, belongs to Osteoglossidae, Guyana of scoop South America and Amazon River, and a kind of important economic fish in local rivers and lakes, is also tropical fresh water pet fish that people like, global.The imperial fish body of silver is long band shape, and flat-sided, it is fan-shaped that tail is, and dorsal fin and anal fin are band shape, extand rearward to caudal peduncle base portion.Lower jaw is more outstanding than maxilla, and length has a pair of short and thick palpus.On its roomy fish body, fitly arranging the large scale of five rows, under light irradiates, the light of the silver color of glimmering.Each fin, under illumination, reflects smooth red or orange gloss.Juvenile fish body colour is more blue; Dorsal fin and anal fin all have reddish blue edge.The imperial scale of gold edge is golden yellow, and each fin is golden yellow redness.Red imperial body colour is golden yellow, and scale edge is golden red, and mouth and the gill cover all have peony patch, and each fin is peony.The imperial body of silver is silvery white in color, and black imperial body colour is silvery white and slightly shallow livid purple look, and each fin is basket black.Dragon fish length can reach 50～100 centimetres.
Because the natural distribution of the imperial fish of silver is restricted, and sexual maturity is slower, and fertility is low.Therefore China every year all will be from a large amount of silver dragon fry of imported from America, and in order to break the situation of the whole dependence on import of China imperial fish of silver, the artificial propagation of silver-colored imperial fish has just become urgent problem with seed rearing.
Summary of the invention
The technical problem that the present invention mainly solves is to provide the induced spawning method of the imperial fish of a kind of silver, and the incubation rate of silver-colored imperial fish fry and survival rate are all higher.
For solving the problems of the technologies described above, the technical scheme that the present invention adopts is:
The induced spawning method of the imperial fish of a kind of silver is provided, comprises the antenatal cultivation of silver-colored imperial fish parent fish and the artificial induced spawning of silver-colored imperial fish, key step is as follows:
(1) the antenatal cultivation of silver-colored imperial fish parent fish:
(101) preparation of aquarium, the imperial fish of silver is healthy and strong large-scale fingerling, the volume of cultivating the aquarium of silver-colored imperial fish need be greater than 1.5m*1m*0.8m, in aquarium, configure oxygen charging pump, case internal circulation filtering device, heating tube and landing-spoon, the water source in aquarium is the running water being exposed to the sun more than 3d through sunlight;
(102) putting in a suitable place to breed of parent population, the stalwartness individuality that selection build is very large, color is pure, dragon palpus is straight and upright, fin ray is complete, anosis nothing is hindered is as parent population, the age of described parent population was for 4 ~ 5 ages, parent population is put into the aquarium of step (101) according to female-male proportion 1:1, female, male parent population is separated respectively with dividing plate, on described dividing plate, offer the hole for the both sides current that circulate;
(103) throwing something and feeding of feed, feed adopts live body feed fresh and that do not crossed by bacterium, parasite and pesticide pollution, and the artitificial food of simultaneously throwing something and feeding is thrown something and fed once up to 9 o'clock in the morning eight respectively, threw something and fed once afternoon six up to 7 o'clock;
(104) daily management, observe that parent population ingests, the situation of change of activity and water quality, record the active situation of the dissolved oxygen in water temperature, water, the forage volume of throwing something and feeding, parent population every day, toward the water filling situation in aquarium and the situation of ingesting of parent population, make regular check on fish body health status, and observe the gonad development situation of parent population;
(2) artificial induced spawning of silver-colored imperial fish:
(201) preparation of hastening parturition, selects the aquarium of 1.5m*1m*0.8m, and every pair of parent population is put in a suitable place to breed in same aquarium, in described aquarium, is provided with artificial aquatic weed;
(202) selection of parent population to be hastened parturition and pairing, ripe female fish length is 70 ~ 80cm, belly is fuller than milter, and the shape of face is mellow and full, and oviduct is outstanding, ripe male fish length is 80 ~ 90cm, pectoral fin is sturdy, and abdomeinal fin point is long, and body is carried on the back higher than raun, vas deferens depression, silver-colored imperial fish parent fish is according to the proportional paired of male and female 1:1;
(203) anesthesia of parent population to be hastened parturition, is used anaesthetic to soak the imperial fish parent fish of parent, carries out the injection of oxytocic drug until parent population during in twilight-sleep state, and described anaesthetic comprises wintermin, yellow Jackets and urethane;
(204) preparation of oxytocic drug and injection, the oxytocic drug adopting has No. 2, short Luteinizing hormone releasing hormone analog and human chorionic gonadtropin, the injected dose of oxytocic drug is short No. 25 μ g/kg of Luteinizing hormone releasing hormone analog and human chorionic gonadtropin 500IU/kg, parenteral solution uses 0.7% sodium chloride solution preparation, and the consumption of parenteral solution is every tail 3 ~ 10mL; The injection of oxytocic drug is injected at twice, injects for the first time 10% of accumulated dose, injects for the second time surplus, the double injection time interval 10 ~ 14h, and injection adopts thoracic cavity injection, two kinds of modes of dorsal fin intramuscular injection;
(205) spawning and fertilization, parent population is put into the case of hastening parturition after the double injection oxytocic drug in step (204), described in the case of hastening parturition take shading measure, in the process of laying eggs, keep hastening parturition the peace and quiet of case surrounding environment.
In a preferred embodiment of the present invention, in step (103), described live body feed comprises the paradise fish of China, goldfish, shrimp, loach, frog, centipede, spider, locust or cockroach, and described artitificial food is advisable with the internal organ of beef or ox.
In a preferred embodiment of the present invention, in step (203), adopt the urethane of 0.8mg/L to soak silver-colored imperial fish parent fish 13min.
In a preferred embodiment of the present invention, in step (204), the degree of depth of thoracic cavity injection is 0.5 ~ 0.6cm, and the dorsal fin intramuscular injection degree of depth is 0.7 ~ 0.8cm.
The invention has the beneficial effects as follows: the present invention comprehensively discloses the induced spawning method of silver-colored imperial fish, comprise the antenatal cultivation of silver-colored imperial fish and the artificial induced spawning of silver-colored imperial fish, use the present invention can ensure that silver-colored imperial fish obtains more fertilized egg, the incubation rate of fertilized egg and the survival rate of fry are all higher, realize silver-colored imperial fish and bred in northern China, there is higher economic worth.
Embodiment
Below preferred embodiment of the present invention is described in detail, thereby so that advantages and features of the invention can be easier to be it will be appreciated by those skilled in the art that, protection scope of the present invention is made to more explicit defining.
An induced spawning method for the imperial fish of silver, comprises the antenatal cultivation of silver-colored imperial fish parent fish and the artificial induced spawning of silver-colored imperial fish, and key step is as follows:
(1) the antenatal cultivation of silver-colored imperial fish parent fish:
(101) preparation of aquarium, the imperial fish of silver is healthy and strong large-scale fingerling, the volume of cultivating the aquarium of silver-colored imperial fish need be greater than 1.5m*1m*0.8m, in aquarium, configure oxygen charging pump, case internal circulation filtering device, heating tube and landing-spoon, the water source in aquarium is the running water being exposed to the sun more than 3d through sunlight;
(102) putting in a suitable place to breed of parent population, the stalwartness individuality that selection build is very large, color is pure, dragon palpus is straight and upright, fin ray is complete, anosis nothing is hindered is as parent population, the age of described parent population was for 4 ~ 5 ages, parent population is put into the aquarium of step (101) according to female-male proportion 1:1, female, male parent population is separated respectively with dividing plate, on described dividing plate, offer the hole for the both sides current that circulate;
(103) throwing something and feeding of feed, feed adopts live body feed fresh and that do not crossed by bacterium, parasite and pesticide pollution, described live body feed comprises the paradise fish of China, goldfish, shrimp, loach, frog, centipede, spider, locust or cockroach, the artitificial food of simultaneously throwing something and feeding, described artitificial food is advisable with the internal organ of beef or ox and is thrown something and fed once up to 9 o'clock in the morning eight respectively, threw something and fed once afternoon six up to 7 o'clock;
(104) daily management, observe that parent population ingests, the situation of change of activity and water quality, record the active situation of the dissolved oxygen in water temperature, water, the forage volume of throwing something and feeding, parent population every day, toward the water filling situation in aquarium and the situation of ingesting of parent population, make regular check on fish body health status, and observe the gonad development situation of parent population;
(2) artificial induced spawning of silver-colored imperial fish:
(201) preparation of hastening parturition, selects the aquarium of 1.5m*1m*0.8m, and every pair of parent population is put in a suitable place to breed in same aquarium, in described aquarium, is provided with artificial aquatic weed;
(202) selection of parent population to be hastened parturition and pairing, ripe female fish length is 70 ~ 80cm, belly is fuller than milter, and the shape of face is mellow and full, and oviduct is outstanding, ripe male fish length is 80 ~ 90cm, pectoral fin is sturdy, and abdomeinal fin point is long, and body is carried on the back higher than raun, vas deferens depression, silver-colored imperial fish parent fish is according to the proportional paired of male and female 1:1;
(203) anesthesia of parent population to be hastened parturition, use anaesthetic to soak the imperial fish parent fish of parent, carry out the injection of oxytocic drug during in twilight-sleep state until parent population, described anaesthetic comprises wintermin, yellow Jackets and urethane, adopts the urethane of 0.8mg/L to soak silver-colored imperial fish parent fish 13min;
(204) preparation of oxytocic drug and injection, the oxytocic drug adopting has No. 2, short Luteinizing hormone releasing hormone analog and human chorionic gonadtropin, the injected dose of oxytocic drug is short No. 25 μ g/kg of Luteinizing hormone releasing hormone analog and human chorionic gonadtropin 500IU/kg, parenteral solution uses 0.7% sodium chloride solution preparation, and the consumption of parenteral solution is every tail 3 ~ 10mL; The injection of oxytocic drug is injected at twice, injects for the first time 10% of accumulated dose, injects for the second time surplus, the double injection time interval 10 ~ 14h, injection adopts thoracic cavity injection, two kinds of modes of dorsal fin intramuscular injection, and the degree of depth of thoracic cavity injection is 0.5 ~ 0.6cm, and the dorsal fin intramuscular injection degree of depth is 0.7 ~ 0.8cm;
(205) spawning and fertilization, parent population is put into the case of hastening parturition after the double injection oxytocic drug in step (204), described in the case of hastening parturition take shading measure, in the process of laying eggs, keep hastening parturition the peace and quiet of case surrounding environment.
In a preferred embodiment of the present invention, in step (103), described live body feed comprises the paradise fish of China, goldfish, shrimp, loach, frog, centipede, spider, locust or cockroach, and described artitificial food is advisable with the internal organ of beef or ox.
The foregoing is only embodiments of the invention; not thereby limit the scope of the claims of the present invention; every equivalent structure or conversion of equivalent flow process that utilizes description of the present invention to do; or be directly or indirectly used in other relevant technical fields, be all in like manner included in scope of patent protection of the present invention.
Claims (4)
1. an induced spawning method for the imperial fish of silver, is characterized in that, comprises the antenatal cultivation of silver-colored imperial fish parent fish and the artificial induced spawning of silver-colored imperial fish, and key step is as follows:
(1) the antenatal cultivation of silver-colored imperial fish parent fish:
(101) preparation of aquarium, the imperial fish of silver is healthy and strong large-scale fingerling, the volume of cultivating the aquarium of silver-colored imperial fish need be greater than 1.5m*1m*0.8m, in aquarium, configure oxygen charging pump, case internal circulation filtering device, heating tube and landing-spoon, the water source in aquarium is the running water being exposed to the sun more than 3d through sunlight;
(102) putting in a suitable place to breed of parent population, the stalwartness individuality that selection build is very large, color is pure, dragon palpus is straight and upright, fin ray is complete, anosis nothing is hindered is as parent population, the age of described parent population was for 4 ~ 5 ages, parent population is put into the aquarium of step (101) according to female-male proportion 1:1, female, male parent population is separated respectively with dividing plate, on described dividing plate, offer the hole for the both sides current that circulate;
(103) throwing something and feeding of feed, feed adopts live body feed fresh and that do not crossed by bacterium, parasite and pesticide pollution, and the artitificial food of simultaneously throwing something and feeding is thrown something and fed once up to 9 o'clock in the morning eight respectively, threw something and fed once afternoon six up to 7 o'clock;
(104) daily management, observe that parent population ingests, the situation of change of activity and water quality, record the active situation of the dissolved oxygen in water temperature, water, the forage volume of throwing something and feeding, parent population every day, toward the water filling situation in aquarium and the situation of ingesting of parent population, make regular check on fish body health status, and observe the gonad development situation of parent population;
(2) artificial induced spawning of silver-colored imperial fish:
(201) preparation of hastening parturition, selects the aquarium of 1.5m*1m*0.8m, and every pair of parent population is put in a suitable place to breed in same aquarium, in described aquarium, is provided with artificial aquatic weed;
(202) selection of parent population to be hastened parturition and pairing, ripe female fish length is 70 ~ 80cm, belly is fuller than milter, and the shape of face is mellow and full, and oviduct is outstanding, ripe male fish length is 80 ~ 90cm, pectoral fin is sturdy, and abdomeinal fin point is long, and body is carried on the back higher than raun, vas deferens depression, silver-colored imperial fish parent fish is according to the proportional paired of male and female 1:1;
(203) anesthesia of parent population to be hastened parturition, is used anaesthetic to soak the imperial fish parent fish of parent, carries out the injection of oxytocic drug until parent population during in twilight-sleep state, and described anaesthetic comprises wintermin, yellow Jackets and urethane;
(204) preparation of oxytocic drug and injection, the oxytocic drug adopting has No. 2, short Luteinizing hormone releasing hormone analog and human chorionic gonadtropin, the injected dose of oxytocic drug is short No. 25 μ g/kg of Luteinizing hormone releasing hormone analog and human chorionic gonadtropin 500IU/kg, parenteral solution uses 0.7% sodium chloride solution preparation, and the consumption of parenteral solution is every tail 3 ~ 10mL; The injection of oxytocic drug is injected at twice, injects for the first time 10% of accumulated dose, injects for the second time surplus, the double injection time interval 10 ~ 14h, and injection adopts thoracic cavity injection, two kinds of modes of dorsal fin intramuscular injection;
(205) spawning and fertilization, parent population is put into the case of hastening parturition after the double injection oxytocic drug in step (204), described in the case of hastening parturition take shading measure, in the process of laying eggs, keep hastening parturition the peace and quiet of case surrounding environment.
2. the induced spawning method of the imperial fish of silver according to claim 1, it is characterized in that, in step (103), described live body feed comprises the paradise fish of China, goldfish, shrimp, loach, frog, centipede, spider, locust or cockroach, and described artitificial food is advisable with the internal organ of beef or ox.
3. the induced spawning method of the imperial fish of silver according to claim 1, is characterized in that, in step (203), adopts the urethane of 0.8mg/L to soak silver-colored imperial fish parent fish 13min.
4. the induced spawning method of the imperial fish of silver according to claim 1, is characterized in that, in step (204), the degree of depth of thoracic cavity injection is 0.5 ~ 0.6cm, and the dorsal fin intramuscular injection degree of depth is 0.7 ~ 0.8cm.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104255608A (en) * | 2014-09-26 | 2015-01-07 | 侯亚军 | Artificial spawning method for thick-lipped hemibarbus labeo |
| CN105165694A (en) * | 2015-11-02 | 2015-12-23 | 山东省冠县合顺渔业专业合作社 | Artificial propagation method for F1 generation of snow arowana |
| CN105830981A (en) * | 2016-04-20 | 2016-08-10 | 柳州市文华养殖场 | Scientific aquaculture method of ornamental fish |
| CN105900879A (en) * | 2016-04-25 | 2016-08-31 | 石家庄市环境监测中心 | Efficient oryzias latipe breeding and hatching method |
| CN107593537A (en) * | 2017-11-14 | 2018-01-19 | 衡阳市九龙生态农业有限公司 | The white shark artificial fecundation method of fresh water |
| CN107950443A (en) * | 2017-12-29 | 2018-04-24 | 西南大学 | A kind of method for improving perch carp postpartum parent population survival rate |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2386248C2 (en) * | 2008-06-23 | 2010-04-20 | Институт проблем экологии и эволюции им. А.С. Северцева Российской академии наук (ИПЭЭ РАН) | Method of spawn incubation in artificial nests-incubators arranged in rivers |
-
2014
- 2014-03-21 CN CN201410105440.3A patent/CN103875567A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2386248C2 (en) * | 2008-06-23 | 2010-04-20 | Институт проблем экологии и эволюции им. А.С. Северцева Российской академии наук (ИПЭЭ РАН) | Method of spawn incubation in artificial nests-incubators arranged in rivers |
Non-Patent Citations (4)
| Title |
|---|
| 吕冬云等: "银龙鱼的人工催产技术", 《水产养殖》, no. 2, 1 February 2009 (2009-02-01), pages 30 - 31 * |
| 庄树文等: "银龙鱼抗病育种技术", 《水产养殖》, vol. 28, no. 5, 1 September 2007 (2007-09-01) * |
| 庄树文等: "银龙鱼的养殖技术及其鉴赏", 《河北渔业》, no. 9, 20 September 2006 (2006-09-20) * |
| 黄俊秀: "银龙的养殖", 《广西水产科技》, 30 August 2006 (2006-08-30) * |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104255608A (en) * | 2014-09-26 | 2015-01-07 | 侯亚军 | Artificial spawning method for thick-lipped hemibarbus labeo |
| CN104255608B (en) * | 2014-09-26 | 2017-02-15 | 侯亚军 | Artificial spawning method for thick-lipped nude hemibarbus labeo |
| CN105165694A (en) * | 2015-11-02 | 2015-12-23 | 山东省冠县合顺渔业专业合作社 | Artificial propagation method for F1 generation of snow arowana |
| CN105165694B (en) * | 2015-11-02 | 2018-05-29 | 山东省冠县合顺渔业专业合作社 | A kind of snow dragonfish F1 generation artificial fecundation method |
| CN105830981A (en) * | 2016-04-20 | 2016-08-10 | 柳州市文华养殖场 | Scientific aquaculture method of ornamental fish |
| CN105900879A (en) * | 2016-04-25 | 2016-08-31 | 石家庄市环境监测中心 | Efficient oryzias latipe breeding and hatching method |
| CN105900879B (en) * | 2016-04-25 | 2018-08-21 | 石家庄市环境监测中心 | A kind of medaka efficiently breeding and hatching method |
| CN107593537A (en) * | 2017-11-14 | 2018-01-19 | 衡阳市九龙生态农业有限公司 | The white shark artificial fecundation method of fresh water |
| CN107950443A (en) * | 2017-12-29 | 2018-04-24 | 西南大学 | A kind of method for improving perch carp postpartum parent population survival rate |
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Application publication date: 20140625 |