CN104686430A - Prawn low dissolved oxygen resistance family selection method - Google Patents
Prawn low dissolved oxygen resistance family selection method Download PDFInfo
- Publication number
- CN104686430A CN104686430A CN201510158892.2A CN201510158892A CN104686430A CN 104686430 A CN104686430 A CN 104686430A CN 201510158892 A CN201510158892 A CN 201510158892A CN 104686430 A CN104686430 A CN 104686430A
- Authority
- CN
- China
- Prior art keywords
- family
- prawn
- dissolved oxygen
- shrimp
- low dissolved
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000238557 Decapoda Species 0.000 title claims abstract description 242
- 239000001301 oxygen Substances 0.000 title claims abstract description 130
- 229910052760 oxygen Inorganic materials 0.000 title claims abstract description 130
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 title claims abstract description 129
- 238000010187 selection method Methods 0.000 title abstract 2
- 230000013011 mating Effects 0.000 claims abstract description 23
- 230000026109 gonad development Effects 0.000 claims abstract description 22
- 230000002068 genetic effect Effects 0.000 claims abstract description 21
- 238000000034 method Methods 0.000 claims abstract description 20
- 230000007613 environmental effect Effects 0.000 claims abstract description 17
- 238000012360 testing method Methods 0.000 claims abstract description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 40
- 230000004083 survival effect Effects 0.000 claims description 22
- 238000002474 experimental method Methods 0.000 claims description 21
- 230000037396 body weight Effects 0.000 claims description 19
- 230000009027 insemination Effects 0.000 claims description 14
- 238000009394 selective breeding Methods 0.000 claims description 13
- 238000004458 analytical method Methods 0.000 claims description 11
- 238000009399 inbreeding Methods 0.000 claims description 10
- 235000019082 Osmanthus Nutrition 0.000 claims description 8
- 241000333181 Osmanthus Species 0.000 claims description 8
- 238000004519 manufacturing process Methods 0.000 claims description 8
- 239000004744 fabric Substances 0.000 claims description 7
- 235000012907 honey Nutrition 0.000 claims description 7
- 230000006872 improvement Effects 0.000 claims description 7
- 239000002085 irritant Substances 0.000 claims description 7
- 231100000021 irritant Toxicity 0.000 claims description 7
- 230000008775 paternal effect Effects 0.000 claims description 7
- 238000005070 sampling Methods 0.000 claims description 7
- 210000002784 stomach Anatomy 0.000 claims description 7
- 238000005516 engineering process Methods 0.000 claims description 5
- 208000005652 acute fatty liver of pregnancy Diseases 0.000 claims description 4
- 230000035945 sensitivity Effects 0.000 claims 1
- 238000009395 breeding Methods 0.000 abstract description 32
- 230000001488 breeding effect Effects 0.000 abstract description 32
- 238000012258 culturing Methods 0.000 abstract description 4
- 238000011161 development Methods 0.000 abstract description 3
- 230000018109 developmental process Effects 0.000 abstract description 3
- 230000035882 stress Effects 0.000 description 12
- 238000010171 animal model Methods 0.000 description 6
- 238000007405 data analysis Methods 0.000 description 6
- 238000007689 inspection Methods 0.000 description 6
- 238000005259 measurement Methods 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 6
- 230000007850 degeneration Effects 0.000 description 4
- 206010021143 Hypoxia Diseases 0.000 description 3
- 241000238553 Litopenaeus vannamei Species 0.000 description 3
- 238000009360 aquaculture Methods 0.000 description 3
- 244000144974 aquaculture Species 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 210000003734 kidney Anatomy 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 208000019888 Circadian rhythm sleep disease Diseases 0.000 description 2
- 208000001456 Jet Lag Syndrome Diseases 0.000 description 2
- 241001124325 Marsupenaeus japonicus Species 0.000 description 2
- 208000007443 Neurasthenia Diseases 0.000 description 2
- 206010003549 asthenia Diseases 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 230000007954 hypoxia Effects 0.000 description 2
- 208000033915 jet lag type circadian rhythm sleep disease Diseases 0.000 description 2
- 238000013508 migration Methods 0.000 description 2
- 230000005012 migration Effects 0.000 description 2
- 230000000050 nutritive effect Effects 0.000 description 2
- 230000005195 poor health Effects 0.000 description 2
- 238000005728 strengthening Methods 0.000 description 2
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 1
- 206010003210 Arteriosclerosis Diseases 0.000 description 1
- 208000006820 Arthralgia Diseases 0.000 description 1
- 241000238421 Arthropoda Species 0.000 description 1
- JEBFVOLFMLUKLF-IFPLVEIFSA-N Astaxanthin Natural products CC(=C/C=C/C(=C/C=C/C1=C(C)C(=O)C(O)CC1(C)C)/C)C=CC=C(/C)C=CC=C(/C)C=CC2=C(C)C(=O)C(O)CC2(C)C JEBFVOLFMLUKLF-IFPLVEIFSA-N 0.000 description 1
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241001235204 Fenneropenaeus merguiensis Species 0.000 description 1
- 241000335026 Fenneropenaeus penicillatus Species 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- 206010062717 Increased upper airway secretion Diseases 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- 241000238555 Malacostraca Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 208000000112 Myalgia Diseases 0.000 description 1
- 206010059013 Nocturnal emission Diseases 0.000 description 1
- 208000002193 Pain Diseases 0.000 description 1
- 241000238550 Penaeidae Species 0.000 description 1
- 241000927735 Penaeus Species 0.000 description 1
- 241000243820 Polychaeta Species 0.000 description 1
- 208000003251 Pruritus Diseases 0.000 description 1
- 206010039897 Sedation Diseases 0.000 description 1
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 1
- 206010040943 Skin Ulcer Diseases 0.000 description 1
- 208000003217 Tetany Diseases 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 1
- 230000001147 anti-toxic effect Effects 0.000 description 1
- 230000036528 appetite Effects 0.000 description 1
- 235000019789 appetite Nutrition 0.000 description 1
- 208000011775 arteriosclerosis disease Diseases 0.000 description 1
- MQZIGYBFDRPAKN-ZWAPEEGVSA-N astaxanthin Chemical compound C([C@H](O)C(=O)C=1C)C(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1=C(C)C(=O)[C@@H](O)CC1(C)C MQZIGYBFDRPAKN-ZWAPEEGVSA-N 0.000 description 1
- 229940022405 astaxanthin Drugs 0.000 description 1
- 235000013793 astaxanthin Nutrition 0.000 description 1
- 239000001168 astaxanthin Substances 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 210000000481 breast Anatomy 0.000 description 1
- 210000000748 cardiovascular system Anatomy 0.000 description 1
- 238000005266 casting Methods 0.000 description 1
- FDJOLVPMNUYSCM-WZHZPDAFSA-L cobalt(3+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+3].N#[C-].N([C@@H]([C@]1(C)[N-]\C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C(\C)/C1=N/C([C@H]([C@@]1(CC(N)=O)C)CCC(N)=O)=C\C1=N\C([C@H](C1(C)C)CCC(N)=O)=C/1C)[C@@H]2CC(N)=O)=C\1[C@]2(C)CCC(=O)NC[C@@H](C)OP([O-])(=O)O[C@H]1[C@@H](O)[C@@H](N2C3=CC(C)=C(C)C=C3N=C2)O[C@@H]1CO FDJOLVPMNUYSCM-WZHZPDAFSA-L 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 210000004351 coronary vessel Anatomy 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000013278 delphi method Methods 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 230000037111 immune power Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 201000001881 impotence Diseases 0.000 description 1
- 229910052740 iodine Inorganic materials 0.000 description 1
- 239000011630 iodine Substances 0.000 description 1
- 231100000225 lethality Toxicity 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000007726 management method Methods 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 208000010125 myocardial infarction Diseases 0.000 description 1
- 210000005036 nerve Anatomy 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
- 208000026435 phlegm Diseases 0.000 description 1
- 230000002028 premature Effects 0.000 description 1
- 206010036596 premature ejaculation Diseases 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 230000036280 sedation Effects 0.000 description 1
- 229910052711 selenium Inorganic materials 0.000 description 1
- 239000011669 selenium Substances 0.000 description 1
- 230000009758 senescence Effects 0.000 description 1
- 235000015170 shellfish Nutrition 0.000 description 1
- 231100000019 skin ulcer Toxicity 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000009182 swimming Effects 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/50—Culture of aquatic animals of shellfish
- A01K61/59—Culture of aquatic animals of shellfish of crustaceans, e.g. lobsters or shrimps
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention relates to a prawn low dissolved oxygen resistance family selection method, and belongs to the technical field of prawn stress-resistance trait selection. The method comprises the steps of base population establishing; F1 generation family establishing; same pool culturing; low dissolved oxygen resistance testing; F2 generation family establishing; continuous generation selecting, wherein different sources of parent prawns are selected to establish a full/half-slib family through directional mating, common environmental breeding is performed, 94 h low dissolved oxygen stress is performed, parent selecting and remaining are performed according to the low dissolved oxygen resistance test result and the growth measure result, after four generations of continuous selection, the family of distant genetic relationship is selected from the families with excellent growth performance and strong low dissolved oxygen resistance performance, the male and female prawns which are big in individual, strong in vigor and normal in gonad development are selected to serve as parent to breed as good varieties. According to the method, the problem occurring in prawn high-density culturing that the prawns are dead caused by the fact that the prawn growth is influenced by low dissolved oxygen is solved, and the development of the prawn high-density culture industry is greatly promoted.
Description
Technical field
The present invention relates to a kind of prawn selection, be specifically related to the resistance to low dissolved oxygen family selective breeding method of a kind of prawn, belong to prawn degeneration-resistant proterties breeding technique field.
Background technology
Prawn belongs to Arthropoda, Branchiata, Crustachia, Malacostraca, Decapoda, swimming suborder, Penaeidae, Penaeus.Prawn can divide sedentariae (as japonicus, furrowed prawn, European prawn etc.) and migration type (as Chinese prawn, banana prawn, penaeus penicillatus), and last class is dwelt in littoral shallow sea, often slips into daytime at husky the end, does not do large-scale movement; A rear class is dwelt in the muddy marine site of bank, river mouth, and normal work is moved and migration on a large scale.Prawn is mainly food with benthonic invertebrates, as Polychaeta, small-sized shell-fish and bivalve etc., sometimes also catches zooplankton.
Prawn has high nutritive value, is the delicious food be loved by the people.Shrimp has tonifying kidney and strengthening yang, logical breast is antitoxin, the controlling nocturnal emission with astringent drugs that nourishes blood, dissolving blood stasis and detoxication, beneficial gas grow sun, remove obstruction in channels to relieve pain, effect such as to reduce phlegm of whetting the appetite, and is suitable for the patients such as impotence due to deficiency of the kidney, seminal emission premature ejaculation, alactation, arthralgia and myalgia, tetany, whole body itch, skin ulcer, in poor health and neurasthenia.Modern medicine study confirms, the high nutritive value of shrimp, can strengthen immunity and the property function of human body, tonifying kidney and strengthening yang, premature senescence resistance; Dried small shrimp has sedation, is commonly used to treat neurasthenia, all diseases of vegetative nerve functional disturbance; Containing three kinds of important fatty acid in shrimp, people can be made to keep energy to concentrate for a long time; Containing a large amount of cobalamin in shrimp, be rich in zinc, iodine and selenium simultaneously, heat and fat lower, nutritious, and its meat is soft, easy to digest, in poor health and need the people taken good care of to be fabulous food after being ill; The abundant magnesium contained in shrimp has important regulating action to cardiomotility, well can protect cardiovascular system, reduces blood cholesterol level, prevents arteriosclerosis, simultaneously can also coronary artery dilator, is conducive to preventing hypertension and myocardial infarction; Astaxanthin in shrimp body contributes to eliminating " jet lag " that produce because of jet lag, has benifit to the healthy pole of the mankind.
China is prawn production state maximum in the world, is also in the world to the Main Trade state of shrimp products, and in recent years, due to the restriction of soil and water resource, intensive highly dense aquaculture model becomes the main aquaculture model of prawn.Dissolved oxygen affects the most important water quality factor of prawn culturing (horse is true to adopt Application of Delphi Method research to find, 2010), due to comprehensive function that is biological and chemical factors, often there is the phenomenon of dissolved oxygen deficiency in high-density breeding shrimp pond, in low dissolved oxygen water body, prawn reduces tolerances such as temperature, salinity, ammonia nitrogen, hydrogen sulphide, declines to virus, bacterial immune power, minimizing growth of casting off a skin slows down, and lethality increases.The resistance to low dissolved oxygen proterties of prawn becomes the important character affecting its growth, survival, but the prawn kind of domestic seed selection: Chinese prawn " Huanghai No.1 ", " No. 2, the Huanghai Sea ", Environment of Litopenaeus vannamei Low " in emerging No. 1 ", " No. 1, middle section ", " No. 1, section sea ", " No. 1, sea, osmanthus ", main or with growth and disease resistance trait for selective goal.
Have been reported in plant and other animals, improve its growth, survival performance by the selection of degeneration-resistant proterties, resistance to low dissolved oxygen proterties, as the degeneration-resistant proterties of one, has important seed selection and is worth.Therefore, invent the selection of a kind of resistance to low dissolved oxygen prawn, cultivate the prawn new varieties of resistance to low dissolved oxygen, occur when effectively solving prawn high-density breeding that low dissolved oxygen affects prawn growth, causes a difficult problem for prawn death, the development of prawn high-density breeding industry will be promoted greatly.
Breeding is the important foundation of culture fishery sustainable development, research about prawn fine-variety breeding never stops, Chinese invention " a kind of selection of Environment of Litopenaeus vannamei Low breeding ", application number 201410039044.5 provides the selection of the Environment of Litopenaeus vannamei Low breeding of a kind of high yield, degeneration-resistant applicable high salinity and low salinity stocking environment; Chinese invention " a kind of Marsupenaeus japonicus family builds and selection ", application number 201110278874.X provides a kind of Marsupenaeus japonicus younger sister cousin-german family and builds the method with seed selection, but the research low for low dissolved oxygen environment prawn culturing survival rate have not been reported.
Summary of the invention
The object of this invention is to provide the family selective breeding method of the resistance to low dissolved oxygen kind of a kind of prawn, to solve in high-density breeding process, the prawn poor growth that water hypoxia causes, the problem that cultivation survival rate is low.
For achieving the above object, the technical solution used in the present invention is as follows:
The resistance to low dissolved oxygen family selective breeding method of a kind of prawn, comprises the steps:
(1) foundation of basic population: collect from Florida of U.S. prawn improvement company SIS, Kona gulf marine resources company Konna Bay, Oceanic Institute of Hawaii OI, Thailand honest Qin Xia company ZD and domestic seed selection emerging No. 1, No. 1, middle section, No. 1 close shrimp in sea, osmanthus as colony, set up 15 mating combinations as seed selection basic population by mass mating;
(2) F1 generation family is built: from above-mentioned 15 mating combinations, by 2% selection intensity, picking individual is large, female shrimp 45 ~ 65g, the individuality of male shrimp 35 ~ 55g, male and female shrimp gonad development is normal, appendage is complete, anosis, honey stomach, to external world irritant reaction responsive, reserves seed for planting, by the mode of artificial insemination as close shrimp, by female-male proportion 2:1, setting up family full-sibs 60 ~ 100, paternal half-sib family 30 ~ 50, is F1 generation family;
(3) cultivate with pond: each F1 generation family prawn gets 400 tail shrimp seedlings in 3m × 1m × 1m net cage respectively from the young shrimp stage, the cleaned and changed net cage screen cloth of every 8 ~ 12d, common environmental cultivation is carried out in the large pond that whole net cage is placed in 20m × 30m × 1.5m;
(4) anti-low dissolved oxygen test: when each F1 generation prawn cultivates February and May in common environmental, from each family, randomly draw 30 tail prawns respectively measure prawn body weight, body is long, body length, uromere is long, body width, sampling prawn is carried out optic stalk collar mark, the prawn marked supports 2d temporarily in shrimp pond, if any dead shrimp, from its place family, randomly draw the measurement of prawn mark be supplemented to 30 tails, experiment prawn is put in the round plastic bucket of 800L, treat that dissolved oxygen in water body is consumed to 1.2mg.L-1 and starts timing by prawn, oxygen ranges by adjusting air valve controls to make water body dissolved oxygen maintain 0.8 ~ 1.2mg.L-1(96 hour preliminary experiment and determines), carry out 94h low dissolved oxygen stress, being placed with water pump in bucket, to maintain upper and lower water body dissolved oxygen even, every 1h detects water body dissolved oxygen situation, every 8h detects prawn survival condition, dead and survive prawn to different time sections during each family dissolved oxygen stress, carry out the trait related SNP locus of gene analysis of anti-low dissolved oxygen,
(5) data analysis: utilize ASReml software, adopt multiple characters individual animal model estimation growth traits and resistance to low dissolved oxygen Trait heritability and genetic correlation, prediction growth traits and resistance to low dissolved oxygen proterties breeding value, result shows: the heritability of body weight is 0.27 ± 0.11(medium heritability), resistance to low dissolved oxygen heredity is 0.13 ± 0.07(medium heritability), between body weight and resistance to low dissolved oxygen, genetic correlation is 0.22 ± 0.09, equal Z-Score inspection significantly (P<0.05), adopt 5 monthly age weight character breeding value weightings 50% to add 5 monthly ages low dissolved oxygen survival breeding value weighting 50% and calculate to obtain selection index, screen 20 high familys of families selecting index as family of reserving seed for planting,
(6) F2 is built for family: large, energetic, normal female shrimp 20 tail of gonad development of picking individual from 20 familys of reserving seed for planting, male shrimp 10 tail is as parent, control inbreeding coefficient less than 0.1, build full sibs (half sibs) family by directional cross, build F2 for family;
(7) subculture is selected: by the successive selection of above-mentioned experiment family through 4 generations, in the family that the excellent resistance to low dissolved oxygen performance of growth performance is strong, select the family that affiliation is far away, therefrom large, energetic, the normal male and female prawn of gonad development of picking individual is as parent, breeds offspring as improved seeds for production application.
The present invention selects multiple genetic background different, and colony based on the prawn colony of growth performance excellence, ensure that abundant genetic diversity; Parent's genetic background is clear, by the mating of setting inbreeding coefficient, effectively avoids occurring inbreeding phenomenon in Breeding Process; Adopt artificial insemination, to set up entirely by the mode of female-male proportion 2:1, family half sibs, can calculate the genetic parameter of resistance to low dissolved oxygen proterties and other proterties, better formulate selection scheme; Carry out in common environmental cultivation February and May, fully take into account the impact of common environmental benefit size on the low dissolved oxygen tolerance of prawn.
The invention has the beneficial effects as follows: adopt the resistance to low dissolved oxygen family selective breeding method of a kind of prawn of the present invention, that can shorten resistance to low dissolved oxygen prawn family forms the cycle, reduce maintenance cost, and convenient management, and can solve in high-density breeding process, the prawn poor growth that water hypoxia causes, the problem that cultivation survival rate is low.
Embodiment
Be described in further details the present invention below by example, these examples are only used for the present invention is described, do not limit the scope of the invention.
The present invention carries out in Zhanjiang, Guangdong Province Hai Wei Aquaculture Corporation:
Embodiment 1
The resistance to low dissolved oxygen family selective breeding method of a kind of prawn, comprises the steps:
(1) foundation of basic population: collect from Florida of U.S. prawn improvement company SIS, Kona gulf marine resources company Konna Bay, Oceanic Institute of Hawaii OI, Thailand honest Qin Xia company ZD and domestic seed selection emerging No. 1, No. 1, middle section, No. 1 close shrimp in sea, osmanthus as colony, set up 15 mating combinations as seed selection basic population by mass mating;
(2) F1 generation family is built: from above-mentioned 15 mating combinations, by 2% selection intensity, picking individual is large, female shrimp 45g, the individuality of male shrimp 35g, female shrimp 300 tail, male shrimp 100 tail, male and female shrimp gonad development is normal, appendage is complete, anosis, honey stomach, to external world irritant reaction responsive, reserves seed for planting, by the mode of artificial insemination as close shrimp, by female-male proportion 2:1, setting up family full-sibs 60, paternal half-sib family 30, is F1 generation family;
(3) cultivate with pond: each F1 generation family prawn gets 400 tail shrimp seedlings in 3m × 1m × 1m net cage respectively from the young shrimp stage, the cleaned and changed net cage screen cloth of every 8d, common environmental cultivation is carried out in the large pond that whole net cage is placed in 20m × 30m × 1.5m;
(4) anti-low dissolved oxygen test: when each F1 generation prawn cultivates February and May in common environmental, from each family, randomly draw 30 tail prawns respectively measure prawn body weight, body is long, body length, uromere is long, body width, sampling prawn is carried out optic stalk collar mark, the prawn marked supports 2d temporarily in shrimp pond, if any dead shrimp, from its place family, randomly draw the measurement of prawn mark be supplemented to 30 tails, experiment prawn is put in the round plastic bucket of 800L, treat that dissolved oxygen in water body is consumed to 1.2mg.L-1 and starts timing by prawn, oxygen ranges by adjusting air valve controls to make water body dissolved oxygen maintain 0.8 ~ 1.2mg.L-1(96 hour preliminary experiment and determines), carry out 94h low dissolved oxygen stress, being placed with water pump in bucket, to maintain upper and lower water body dissolved oxygen even, every 1h detects water body dissolved oxygen situation, every 8h detects prawn survival condition, dead and survive prawn to different time sections during each family dissolved oxygen stress, carry out the trait related SNP locus of gene analysis of anti-low dissolved oxygen,
(5) data analysis: utilize ASReml software, adopts multiple characters individual animal model estimation growth traits and resistance to low dissolved oxygen Trait heritability and genetic correlation, predicts growth traits and resistance to low dissolved oxygen proterties breeding value.Result shows: the heritability of body weight is 0.27 ± 0.11(medium heritability), resistance to low dissolved oxygen heredity is 0.13 ± 0.07(medium heritability), between body weight and resistance to low dissolved oxygen, genetic correlation is 0.22 ± 0.09, and equal Z-Score inspection significantly (P<0.05).Adopt 5 monthly age weight character breeding value weightings 50% to add 5 monthly ages low dissolved oxygen survival breeding value weighting 50% and calculate to obtain selection index, screen the high family of families selecting index as family of reserving seed for planting.
(6) build F2 for family: large, energetic, normal female shrimp 20 tail of gonad development of picking individual from 20 familys of reserving seed for planting, male shrimp 10 tail, as parent, controls inbreeding coefficient less than 0.1, by the mode of artificial insemination, builds F2 for family;
(7) subculture is selected: by the successive selection of above-mentioned experiment family through 4 generations, in the family that the excellent resistance to low dissolved oxygen performance of growth performance is strong, select the family that affiliation is far away, therefrom large, energetic, the normal male and female prawn of gonad development of picking individual is as parent, breeds offspring as improved seeds for production application.
Embodiment 2
The resistance to low dissolved oxygen family selective breeding method of a kind of prawn, comprises the steps:
(1) foundation of basic population: collect from Florida of U.S. prawn improvement company SIS, Kona gulf marine resources company Konna Bay, Oceanic Institute of Hawaii OI, Thailand honest Qin Xia company ZD and domestic seed selection emerging No. 1, No. 1, middle section, No. 1 close shrimp in sea, osmanthus is as colony, adopt the technology of AFLP to carry out analysis of genetic diversity to separate sources parent shrimp, set up 15 mating combinations as seed selection basic population by mass mating;
(2) F1 generation family is built: from above-mentioned 15 mating combinations, by 2% selection intensity, picking individual is large, female shrimp 65g, the individuality of male shrimp 55g, female shrimp 400 tail, male shrimp 200 tail, male and female shrimp gonad development is normal, appendage is complete, anosis, honey stomach, to external world irritant reaction responsive, reserves seed for planting, by the mode of artificial insemination as close shrimp, by female-male proportion 2:1, setting up family full-sibs 100, paternal half-sib family 50, is F1 generation family;
(3) cultivate with pond: each F1 generation family prawn gets 400 tail shrimp seedlings in 3m × 1m × 1m net cage respectively from the young shrimp stage, the cleaned and changed net cage screen cloth of every 12d, common environmental cultivation is carried out in the large pond that whole net cage is placed in 20m × 30m × 1.5m;
(4) anti-low dissolved oxygen test: when each F1 generation prawn cultivates February and May in common environmental, from each family, randomly draw 30 tail prawns respectively measure prawn body weight, body is long, body length, uromere is long, body width, sampling prawn is carried out optic stalk collar mark, the prawn marked supports 2d temporarily in shrimp pond, if any dead shrimp, from its place family, randomly draw the measurement of prawn mark be supplemented to 30 tails, experiment prawn is put in the round plastic bucket of 800L, treat that dissolved oxygen in water body is consumed to 1.2mg.L-1 and starts timing by prawn, oxygen ranges by adjusting air valve controls to make water body dissolved oxygen maintain 0.8 ~ 1.2mg.L-1(96 hour preliminary experiment and determines), carry out 94h low dissolved oxygen stress, being placed with water pump in bucket, to maintain upper and lower water body dissolved oxygen even, every 1h detects water body dissolved oxygen situation, every 8h detects prawn survival condition, dead and survive prawn to different time sections during each family dissolved oxygen stress, carry out the trait related SNP locus of gene analysis of anti-low dissolved oxygen,
(5) data analysis: utilize ASReml software, adopts multiple characters individual animal model estimation growth traits and resistance to low dissolved oxygen Trait heritability and genetic correlation, predicts growth traits and resistance to low dissolved oxygen proterties breeding value.Result shows: the heritability of body weight is 0.27 ± 0.11(medium heritability), resistance to low dissolved oxygen heredity is 0.13 ± 0.07(medium heritability), between body weight and resistance to low dissolved oxygen, genetic correlation is 0.22 ± 0.09, and equal Z-Score inspection significantly (P<0.05).Adopt 5 monthly age weight character breeding value weightings 50% to add 5 monthly ages low dissolved oxygens survival breeding value weighting 50% and calculate to obtain selection index, the conduct that screening families selecting index is high is reserved seed for planting family.
(6) build F2 for family: large, energetic, normal female shrimp 20 tail of gonad development of picking individual from family of reserving seed for planting, male shrimp 10 tail, as parent, controls inbreeding coefficient less than 0.1, by the mode of artificial insemination, builds F2 for family;
(7) subculture is selected: by the successive selection of above-mentioned experiment family through 4 generations, in the family that the excellent resistance to low dissolved oxygen performance of growth performance is strong, select the family that affiliation is far away, therefrom large, energetic, the normal male and female prawn of gonad development of picking individual is as parent, breeds offspring as improved seeds for production application.
The resistance to low dissolved oxygen family selective breeding method of embodiment 3 one kinds of prawns, comprise the steps: the foundation of (1) basic population: collect from Florida of U.S. prawn improvement company SIS, Kona gulf marine resources company Konna Bay, Oceanic Institute of Hawaii OI, Thailand honest Qin Xia company ZD and domestic seed selection emerging No. 1, No. 1, middle section, No. 1 close shrimp in sea, osmanthus as colony, set up 15 mating combinations as seed selection basic population by mass mating, (2) F1 generation family is built: from above-mentioned 15 mating combinations, by 2% selection intensity, picking individual is large, female shrimp 55g, the individuality of male shrimp 45g, female shrimp 400 tail, male shrimp 150 tail, male and female shrimp gonad development is normal, appendage is complete, anosis, honey stomach, to external world irritant reaction responsive, reserves seed for planting, by the mode of artificial insemination as close shrimp, by female-male proportion 2:1, setting up family full-sibs 80, paternal half-sib family 40, is F1 generation family, (3) cultivate with pond: each F1 generation family prawn gets 400 tail shrimp seedlings in 3m × 1m × 1m net cage respectively from the young shrimp stage, the cleaned and changed net cage screen cloth of every 10d, common environmental cultivation is carried out in the large pond that whole net cage is placed in 20m × 30m × 1.5m, (4) anti-low dissolved oxygen test: when each F1 generation prawn cultivates February and May in common environmental, from each family, randomly draw 30 tail prawns respectively measure prawn body weight, body is long, body length, uromere is long, body width, sampling prawn is carried out optic stalk collar mark, the prawn marked supports 2d temporarily in shrimp pond, if any dead shrimp, from its place family, randomly draw the measurement of prawn mark be supplemented to 30 tails, experiment prawn is put in the round plastic bucket of 800L, treat that dissolved oxygen in water body is consumed to 1.2mg.L-1 and starts timing by prawn, oxygen ranges by adjusting air valve controls to make water body dissolved oxygen maintain 0.8 ~ 1.2mg.L-1(96 hour preliminary experiment and determines), carry out 94h low dissolved oxygen stress, being placed with water pump in bucket, to maintain upper and lower water body dissolved oxygen even, every 1h detects water body dissolved oxygen situation, every 8h detects prawn survival condition, dead and survive prawn to different time sections during each family dissolved oxygen stress, carry out the trait related SNP locus of gene analysis of anti-low dissolved oxygen,
(5) data analysis: utilize ASReml software, adopts multiple characters individual animal model estimation growth traits and resistance to low dissolved oxygen Trait heritability and genetic correlation, predicts growth traits and resistance to low dissolved oxygen proterties breeding value.Result shows: the heritability of body weight is 0.27 ± 0.11(medium heritability), resistance to low dissolved oxygen heredity is 0.13 ± 0.07(medium heritability), between body weight and resistance to low dissolved oxygen, genetic correlation is 0.22 ± 0.09, and equal Z-Score inspection significantly (P<0.05).Adopt 5 monthly age weight character breeding value weightings 50% to add 5 monthly ages low dissolved oxygen survival breeding value weighting 50% and calculate to obtain selection index, screen 20 higher familys of families selecting index as family of reserving seed for planting.
(6) build F2 for family: large, energetic, normal female shrimp 20 tail of gonad development of picking individual from family of reserving seed for planting, male shrimp 10 tail, as parent, controls inbreeding coefficient less than 0.1, by the mode of artificial insemination, builds F2 for family;
(7) subculture is selected: by the successive selection of above-mentioned experiment family through 4 generations, in the family that the excellent resistance to low dissolved oxygen performance of growth performance is strong, select the family that affiliation is far away, therefrom large, energetic, the normal male and female prawn of gonad development of picking individual is as parent, breeds offspring as improved seeds for production application.
Embodiment 4
The resistance to low dissolved oxygen family selective breeding method of a kind of prawn, comprises the steps:
(1) foundation of basic population: collect from Florida of U.S. prawn improvement company SIS, Kona gulf marine resources company Konna Bay, Oceanic Institute of Hawaii OI, Thailand honest Qin Xia company ZD and domestic seed selection emerging No. 1, No. 1, middle section, No. 1 close shrimp in sea, osmanthus is as colony, adopt the technology of AFLP to carry out analysis of genetic diversity to separate sources parent shrimp, set up 15 mating combinations as seed selection basic population by mass mating;
(2) F1 generation family is built: from above-mentioned 15 mating combinations, by 2% selection intensity, picking individual is large, female shrimp 50g, the individuality of male shrimp 40g, female shrimp 350 tail, male shrimp 150 tail, male and female shrimp gonad development is normal, appendage is complete, anosis, honey stomach, to external world irritant reaction responsive, reserves seed for planting, by the mode of artificial insemination as close shrimp, by female-male proportion 2:1, setting up family full-sibs 70, paternal half-sib family 35, is F1 generation family;
(3) cultivate with pond: each F1 generation family prawn gets 400 tail shrimp seedlings in 3m × 1m × 1m net cage respectively from the young shrimp stage, the cleaned and changed net cage screen cloth of every 9d, common environmental cultivation is carried out in the large pond that whole net cage is placed in 20m × 30m × 1.5m;
(4) anti-low dissolved oxygen test: when each F1 generation prawn cultivates February and May in common environmental, from each family, randomly draw 30 tail prawns respectively measure prawn body weight, body is long, body length, uromere is long, body width, sampling prawn is carried out optic stalk collar mark, the prawn marked supports 2d temporarily in shrimp pond, if any dead shrimp, from its place family, randomly draw the measurement of prawn mark be supplemented to 30 tails, experiment prawn is put in the round plastic bucket of 800L, treat that dissolved oxygen in water body is consumed to 1.2mg.L-1 and starts timing by prawn, oxygen ranges by adjusting air valve controls to make water body dissolved oxygen maintain 0.8 ~ 1.2mg.L-1(96 hour preliminary experiment and determines), carry out 94h low dissolved oxygen stress, being placed with water pump in bucket, to maintain upper and lower water body dissolved oxygen even, every 1h detects water body dissolved oxygen situation, every 8h detects prawn survival condition, dead and survive prawn to different time sections during each family dissolved oxygen stress, carry out the trait related SNP locus of gene analysis of anti-low dissolved oxygen,
(5) data analysis: utilize ASReml software, adopts multiple characters individual animal model estimation growth traits and resistance to low dissolved oxygen Trait heritability and genetic correlation, predicts growth traits and resistance to low dissolved oxygen proterties breeding value.Result shows: the heritability of body weight is 0.27 ± 0.11(medium heritability), resistance to low dissolved oxygen heredity is 0.13 ± 0.07(medium heritability), between body weight and resistance to low dissolved oxygen, genetic correlation is 0.22 ± 0.09, and equal Z-Score inspection significantly (P<0.05).Adopt 5 monthly age weight character breeding value weightings 50% to add 5 monthly ages low dissolved oxygen survival breeding value weighting 50% and calculate to obtain selection index, screen 20 high familys of families selecting index as family of reserving seed for planting.
(6) build F2 for family: large, energetic, normal female shrimp 20 tail of gonad development of picking individual from 20 familys of reserving seed for planting, male shrimp 10 tail, as parent, controls inbreeding coefficient less than 0.1, by the mode of artificial insemination, builds F2 for family;
(7) subculture is selected: by the successive selection of above-mentioned experiment family through 4 generations, in the family that the excellent resistance to low dissolved oxygen performance of growth performance is strong, select the family that affiliation is far away, therefrom large, energetic, the normal male and female prawn of gonad development of picking individual is as parent, breeds offspring as improved seeds for production application.
Embodiment 5
The resistance to low dissolved oxygen family selective breeding method of a kind of prawn, comprises the steps:
(1) foundation of basic population: collect from Florida of U.S. prawn improvement company SIS, Kona gulf marine resources company Konna Bay, Oceanic Institute of Hawaii OI, Thailand honest Qin Xia company ZD and domestic seed selection emerging No. 1, No. 1, middle section, No. 1 close shrimp in sea, osmanthus is as colony, adopt the technology of AFLP to carry out analysis of genetic diversity to separate sources parent shrimp, set up 15 mating combinations as seed selection basic population by mass mating;
(2) F1 generation family is built: from above-mentioned 15 mating combinations, by 2% selection intensity, picking individual is large, female shrimp 60g, the individuality of male shrimp 50g, female shrimp 300 tail, male shrimp 150 tail, male and female shrimp gonad development is normal, appendage is complete, anosis, honey stomach, to external world irritant reaction responsive, reserves seed for planting, by the mode of artificial insemination as close shrimp, by female-male proportion 2:1, setting up family full-sibs 90, paternal half-sib family 45, is F1 generation family;
(3) cultivate with pond: each F1 generation family prawn gets 400 tail shrimp seedlings in 3m × 1m × 1m net cage respectively from the young shrimp stage, the cleaned and changed net cage screen cloth of every 11d, common environmental cultivation is carried out in the large pond that whole net cage is placed in 20m × 30m × 1.5m;
(4) anti-low dissolved oxygen test: when each F1 generation prawn cultivates February and May in common environmental, from each family, randomly draw 30 tail prawns respectively measure prawn body weight, body is long, body length, uromere is long, body width, sampling prawn is carried out optic stalk collar mark, the prawn marked supports 2d temporarily in shrimp pond, if any dead shrimp, from its place family, randomly draw the measurement of prawn mark be supplemented to 30 tails, experiment prawn is put in the round plastic bucket of 800L, treat that dissolved oxygen in water body is consumed to 1.2mg.L-1 and starts timing by prawn, oxygen ranges by adjusting air valve controls to make water body dissolved oxygen maintain 0.8 ~ 1.2mg.L-1(96 hour preliminary experiment and determines), carry out 94h low dissolved oxygen stress, being placed with water pump in bucket, to maintain upper and lower water body dissolved oxygen even, every 1h detects water body dissolved oxygen situation, every 8h detects prawn survival condition, dead and survive prawn to different time sections during each family dissolved oxygen stress, carry out the trait related SNP locus of gene analysis of anti-low dissolved oxygen,
(5) data analysis: utilize ASReml software, adopts multiple characters individual animal model estimation growth traits and resistance to low dissolved oxygen Trait heritability and genetic correlation, predicts growth traits and resistance to low dissolved oxygen proterties breeding value.Result shows: the heritability of body weight is 0.27 ± 0.11(medium heritability), resistance to low dissolved oxygen heredity is 0.13 ± 0.07(medium heritability), between body weight and resistance to low dissolved oxygen, genetic correlation is 0.22 ± 0.09, and equal Z-Score inspection significantly (P<0.05).Adopt 5 monthly age weight character breeding value weightings 50% to add 5 monthly ages low dissolved oxygen survival breeding value weighting 50% and calculate to obtain selection index, screen 20 high familys of families selecting index as family of reserving seed for planting.
(6) build F2 for family: large, energetic, normal female shrimp 20 tail of gonad development of picking individual from family of reserving seed for planting, male shrimp 10 tail, as parent, controls inbreeding coefficient less than 0.1, by the mode of artificial insemination, builds F2 for family;
(7) subculture is selected: by the successive selection of above-mentioned experiment family through 4 generations, in the family that the excellent resistance to low dissolved oxygen performance of growth performance is strong, select the family that affiliation is far away, therefrom large, energetic, the normal male and female prawn of gonad development of picking individual is as parent, breeds offspring as improved seeds for production application.
The resistance to low dissolved oxygen family that the present invention filters out is significantly higher than survival rate average in the survival rate of low-oxygen environment, survival rate improves and within 25.72%, 2014, cultivates that this kind parent shrimp breeding populations is higher than control population survival rate by 21.42% 5000 mu of high-density breeding conditions, output increased 23.74%.
Claims (3)
1. the resistance to low dissolved oxygen family selective breeding method of prawn, is characterized in that: comprise the steps:
(1) foundation of basic population: collect from Florida of U.S. prawn improvement company SIS, Kona gulf marine resources company Konna Bay, Oceanic Institute of Hawaii OI, Thailand honest Qin Xia company ZD and domestic seed selection emerging No. 1, No. 1, middle section, No. 1 close shrimp in sea, osmanthus is as colony, adopt the technology of AFLP to carry out analysis of genetic diversity to separate sources parent shrimp, set up 15 mating combinations as seed selection basic population by mass mating;
(2) build F1 generation family: from above-mentioned 15 mating combinations, by 2% selection intensity, the conduct parent shrimp that picking individual is large is reserved seed for planting, by the mode of artificial insemination, according to female-male proportion 2:1, set up family full-sibs 60 ~ 100, paternal half-sib family 30 ~ 50, as F1 generation family;
(3) cultivate with pond: each F1 generation family prawn gets 400 tail shrimp seedlings in 3m × 1m × 1m net cage respectively from the young shrimp stage, the cleaned and changed net cage screen cloth of every 8 ~ 12d, common environmental cultivation is carried out in the large pond that whole net cage is placed in 20m × 30m × 1.5m;
(4) anti-low dissolved oxygen test: each F1 generation prawn in common environmental, cultivate 2 months and 5 months time, from each family, randomly draw 30 tail prawns respectively measure prawn body weight, body length, body length, uromere length, body width, sampling prawn is carried out optic stalk collar mark, the prawn marked supports 2d temporarily in shrimp pond, then experiment prawn is put in the round plastic bucket of 800L, treat that dissolved oxygen in water body is consumed to 1.2mg.L-1 and starts timing by prawn, control to make water body dissolved oxygen maintain 0.8 ~ 1.2mg.L by adjusting air valve
-1, carry out 94h low dissolved oxygen stress, be placed with water pump in bucket and maintain upper and lower water body dissolved oxygen evenly, every 1h detects water body dissolved oxygen situation, and every 8h detects prawn survival condition, filters out the family that the anti-low dissolved oxygen ability of growth performance excellence is strong, line item analysis of going forward side by side;
(6) build F2 for family: large, energetic, normal female shrimp 20 tail of gonad development of picking individual from family of reserving seed for planting, male shrimp 10 tail, as parent, controls inbreeding coefficient less than 0.1, by the mode of artificial insemination, builds F2 for family;
(7) subculture is selected: by the successive selection of above-mentioned experiment family through 4 generations, in the family that the excellent resistance to low dissolved oxygen performance of growth performance is strong, select the family that affiliation is far away, therefrom large, energetic, the normal male and female prawn of gonad development of picking individual is as parent, breeds offspring as improved seeds for production application.
2. the resistance to low dissolved oxygen family selective breeding method of a kind of prawn according to claim 1, it is characterized in that: the described close shrimp of step (2) refers to individual large, female shrimp 45 ~ 65g, male shrimp 35 ~ 55g, male and female shrimp gonad development is normal, appendage is complete, the individuality of anosis, honey stomach, to external world irritant reaction sensitivity.
3. the resistance to low dissolved oxygen family selective breeding method of a kind of prawn according to claim 1, is characterized in that: the female-male proportion of the described experiment family of step (5) is 2:1, and inbreeding coefficient controls below 0.1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510158892.2A CN104686430B (en) | 2015-04-07 | 2015-04-07 | A kind of resistance to low dissolved oxygen family selective breeding method of prawn |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510158892.2A CN104686430B (en) | 2015-04-07 | 2015-04-07 | A kind of resistance to low dissolved oxygen family selective breeding method of prawn |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104686430A true CN104686430A (en) | 2015-06-10 |
| CN104686430B CN104686430B (en) | 2018-02-16 |
Family
ID=53334330
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510158892.2A Active CN104686430B (en) | 2015-04-07 | 2015-04-07 | A kind of resistance to low dissolved oxygen family selective breeding method of prawn |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104686430B (en) |
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104982371A (en) * | 2015-06-18 | 2015-10-21 | 广东海洋大学 | Different-family common-environment culturing system and method for early breeding stage of prawns |
| CN105393967A (en) * | 2015-12-10 | 2016-03-16 | 浙江海洋学院 | Penaeus prawn sprout cold-resistance performance grade division and optimal selection method |
| CN105519461A (en) * | 2015-12-10 | 2016-04-27 | 浙江海洋学院 | Flow velocity resistance grading and optimal-selection method for juvenile Marsupenaeus japonicus |
| CN108522383A (en) * | 2018-04-26 | 2018-09-14 | 海南归耘田农业科技有限公司 | A kind of breeding method keeping Procambius clarkii merit |
| CN110063284A (en) * | 2019-05-23 | 2019-07-30 | 中国水产科学研究院南海水产研究所 | A kind of breeding method of resistance to roughage Penaeus monodon new varieties (being) |
| CN110250052A (en) * | 2019-07-04 | 2019-09-20 | 淮阴师范学院 | A kind of selection of ussuriensis lower oxygen concentration resistance family |
| CN110710481A (en) * | 2019-12-03 | 2020-01-21 | 江苏省海洋水产研究所 | Method for selecting seeds of palaemon carinicauda with low oxygen resistance |
| CN111226839A (en) * | 2020-03-25 | 2020-06-05 | 中国水产科学研究院淡水渔业研究中心 | Method for breeding hypoxia-resistant hybrid pelteobagrus fulvidraco |
| CN112021226A (en) * | 2020-09-22 | 2020-12-04 | 中国水产科学研究院黄海水产研究所 | Method for expanding breeding of freshwater economic crayfish with low egg laying amount |
| CN113678767A (en) * | 2021-08-10 | 2021-11-23 | 中国水产科学研究院黄海水产研究所 | Breeding method for prawn disease resistance character |
| CN113796342A (en) * | 2020-06-15 | 2021-12-17 | 中国水产科学研究院黄海水产研究所 | Multistage standardized breeding method in shrimp family |
| CN114403068A (en) * | 2022-01-20 | 2022-04-29 | 上海市水产研究所（上海市水产技术推广站） | Low-salt-tolerance palaemon carinicauda strain breeding method |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1526281A (en) * | 2003-03-05 | 2004-09-08 | 中国科学院海洋研究所 | Method of establishing bred prawn pedigree and selectively breeding improved prawn variety |
| CN1957684A (en) * | 2006-11-14 | 2007-05-09 | 中国水产科学研究院黄海水产研究所 | Method for establishing selection index for breeding Chinese prawn in multiple characters |
| JP2008193987A (en) * | 2007-02-15 | 2008-08-28 | Fisheries Research Agency | Crustacean larva raising method |
| US20100162964A1 (en) * | 2008-12-29 | 2010-07-01 | Dalian Fisheries University | Process for polycross breeding Manila clam pedigree |
| CN102067821A (en) * | 2010-10-22 | 2011-05-25 | 中国水产科学研究院黄海水产研究所 | Method for breeding high-ammonia-nitrogen and high pH resistant species of prawn |
| CN102845342A (en) * | 2012-09-14 | 2013-01-02 | 中国水产科学研究院黄海水产研究所 | Stocking and mating method for fish and shrimp group |
-
2015
- 2015-04-07 CN CN201510158892.2A patent/CN104686430B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1526281A (en) * | 2003-03-05 | 2004-09-08 | 中国科学院海洋研究所 | Method of establishing bred prawn pedigree and selectively breeding improved prawn variety |
| CN1957684A (en) * | 2006-11-14 | 2007-05-09 | 中国水产科学研究院黄海水产研究所 | Method for establishing selection index for breeding Chinese prawn in multiple characters |
| JP2008193987A (en) * | 2007-02-15 | 2008-08-28 | Fisheries Research Agency | Crustacean larva raising method |
| US20100162964A1 (en) * | 2008-12-29 | 2010-07-01 | Dalian Fisheries University | Process for polycross breeding Manila clam pedigree |
| CN102067821A (en) * | 2010-10-22 | 2011-05-25 | 中国水产科学研究院黄海水产研究所 | Method for breeding high-ammonia-nitrogen and high pH resistant species of prawn |
| CN102845342A (en) * | 2012-09-14 | 2013-01-02 | 中国水产科学研究院黄海水产研究所 | Stocking and mating method for fish and shrimp group |
Non-Patent Citations (4)
| Title |
|---|
| 孟鹏 等: "中国对虾家系水平遗传多样性的AFLP分析", 《海洋水产研究》 * |
| 王浩 等: "凡纳滨对虾多个引进群体的杂交配合力分析", 《水产学报》 * |
| 薛姝雯 等: "基于ＡＦＬＰ技术的凡纳滨对虾遗传多样性研究", 《西北农林科技大学学报（自然科学版）》 * |
| 陈锚 等: "凡纳滨对虾的选育与家系的建立", 《海洋科学》 * |
Cited By (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104982371A (en) * | 2015-06-18 | 2015-10-21 | 广东海洋大学 | Different-family common-environment culturing system and method for early breeding stage of prawns |
| CN105393967A (en) * | 2015-12-10 | 2016-03-16 | 浙江海洋学院 | Penaeus prawn sprout cold-resistance performance grade division and optimal selection method |
| CN105519461A (en) * | 2015-12-10 | 2016-04-27 | 浙江海洋学院 | Flow velocity resistance grading and optimal-selection method for juvenile Marsupenaeus japonicus |
| CN105393967B (en) * | 2015-12-10 | 2018-12-25 | 浙江海洋学院 | A kind of grade classification and preferred method of Marsupenaeus japonicus shrimp seedling cold tolerance |
| CN105519461B (en) * | 2015-12-10 | 2018-12-25 | 浙江海洋学院 | A kind of grade classification and preferred method of Marsupenaeus japonicus shrimp seedling anti-flow performance |
| CN108522383A (en) * | 2018-04-26 | 2018-09-14 | 海南归耘田农业科技有限公司 | A kind of breeding method keeping Procambius clarkii merit |
| CN110063284B (en) * | 2019-05-23 | 2021-10-08 | 中国水产科学研究院南海水产研究所 | Method for breeding new variety (line) of penaeus monodon with crude feed resistance |
| CN110063284A (en) * | 2019-05-23 | 2019-07-30 | 中国水产科学研究院南海水产研究所 | A kind of breeding method of resistance to roughage Penaeus monodon new varieties (being) |
| CN110250052A (en) * | 2019-07-04 | 2019-09-20 | 淮阴师范学院 | A kind of selection of ussuriensis lower oxygen concentration resistance family |
| CN110250052B (en) * | 2019-07-04 | 2022-04-26 | 淮阴师范学院 | Breeding method for pseudobagrus ussuriensis hypoxia-resistant family |
| CN110710481A (en) * | 2019-12-03 | 2020-01-21 | 江苏省海洋水产研究所 | Method for selecting seeds of palaemon carinicauda with low oxygen resistance |
| CN110710481B (en) * | 2019-12-03 | 2021-10-29 | 江苏省海洋水产研究所 | Method for selecting seeds of palaemon carinicauda with low oxygen resistance |
| CN111226839A (en) * | 2020-03-25 | 2020-06-05 | 中国水产科学研究院淡水渔业研究中心 | Method for breeding hypoxia-resistant hybrid pelteobagrus fulvidraco |
| CN111226839B (en) * | 2020-03-25 | 2021-10-19 | 中国水产科学研究院淡水渔业研究中心 | Method for breeding hypoxia-resistant hybrid pelteobagrus fulvidraco |
| CN113796342B (en) * | 2020-06-15 | 2022-07-08 | 中国水产科学研究院黄海水产研究所 | Multistage standardized breeding method in shrimp family |
| CN113796342A (en) * | 2020-06-15 | 2021-12-17 | 中国水产科学研究院黄海水产研究所 | Multistage standardized breeding method in shrimp family |
| CN112021226A (en) * | 2020-09-22 | 2020-12-04 | 中国水产科学研究院黄海水产研究所 | Method for expanding breeding of freshwater economic crayfish with low egg laying amount |
| CN112021226B (en) * | 2020-09-22 | 2021-09-03 | 中国水产科学研究院黄海水产研究所 | Method for expanding breeding of freshwater economic crayfish with low egg laying amount |
| CN113678767A (en) * | 2021-08-10 | 2021-11-23 | 中国水产科学研究院黄海水产研究所 | Breeding method for prawn disease resistance character |
| CN114403068A (en) * | 2022-01-20 | 2022-04-29 | 上海市水产研究所（上海市水产技术推广站） | Low-salt-tolerance palaemon carinicauda strain breeding method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104686430B (en) | 2018-02-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104686430B (en) | A kind of resistance to low dissolved oxygen family selective breeding method of prawn | |
| CN101919359B (en) | Method for comprehensively regulating and controlling gonad mature ovulation of cheilinus undulates | |
| CN105145419B (en) | A kind of breeding method for obtaining strain line hippocampus heterosis, hybrid vigor | |
| CN104982355A (en) | Cultivation method with high production of grass carp in pool | |
| CN101720705B (en) | Culture method of orange adductor scallop | |
| CN104996330B (en) | A kind of Penaeus Vannmei segmented cultural method based on shrimp age | |
| CN105028273A (en) | Method for artificial domestication and cultivation of semilabeo notabilis | |
| CN101884311B (en) | Method for constructing tongue sole families and breeding superior families | |
| CN103493767B (en) | Odontobulis mpotamophila and Odontobutis yaluensis cross breeding method | |
| CN101627737B (en) | Method for establishing blue crab family and selecting improved breed | |
| CN107864901A (en) | A kind of large-scale floating purse seine fish ecology mixed cultivation method | |
| Hongtuo et al. | Culture of the oriental river prawn (Macrobrachium nipponense) | |
| CN104782554B (en) | A kind of subregion feeding method for being applied to raise together with pond | |
| CN104542456A (en) | Imitative ecological breeding method of giant salamanders | |
| CN101897307B (en) | Carp family selective breeding method utilizing net cage | |
| CN103814853B (en) | A kind of selection of import prawn natural selection | |
| CN106376500B (en) | The thin squama schizothoracin artificial fecundation of Yalongjiang River releases method | |
| CN105475187B (en) | A kind of mixed cultivation method of hybridized prussian carp and Penaeus Vannmei | |
| CN105028262B (en) | A kind of Penaeus Vannmei family half sibs construction method | |
| CN107372230A (en) | A kind of artificial fecundation method of lip dace | |
| CN104322420B (en) | The method for optimizing of the agreeable to the taste bait algae of a kind of freshwater shellfish anodonta woodiana pacifica children freshwater mussel | |
| CN104488759B (en) | A kind of method of molecule assist-breeding grass carp excellent strain and Breeding Effect checking | |
| CN107306856A (en) | A kind of pomfret cage culture method | |
| CN106508759A (en) | Method for synchronous development control of scapharca broughtonii in different geographical populations | |
| CN106172095B (en) | Breeding method of large-size Yangtze river system river crab fine breeds |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB02 | Change of applicant information |
Address after: 524000 Guangdong city of Zhanjiang province Leizhou City Qin Dou Zhen Hou Hong Cun Applicant after: Guangdong Haiwei aquiculture Co., Ltd. Address before: 524254 Guangdong city of Zhanjiang province Leizhou City Qin Dou Zhen Hou Hong Cun Applicant before: Leizhou City Hai Wei aquaculture Co., Ltd |
|
| CB02 | Change of applicant information | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CP03 | Change of name, title or address |
Address after: 524000 Tianshen village, Jijia Town, Leizhou City, Zhanjiang City, Guangdong Province Patentee after: Guangdong Haiwei Agricultural Group Co.,Ltd. Address before: 524000 Houhong village, Qindou Town, Leizhou City, Zhanjiang City, Guangdong Province Patentee before: GUANGDONG HAVWII AQUACULTURE Co.,Ltd. |
|
| CP03 | Change of name, title or address |