CN106577388A - Cross breeding method for Pelteobagrus fulvidraco variety namely Huangyou No. 1 with improved growth character - Google Patents
Cross breeding method for Pelteobagrus fulvidraco variety namely Huangyou No. 1 with improved growth character Download PDFInfo
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Abstract
The invention provides a cross breeding method for a Pelteobagrus fulvidraco variety namely Huangyou No. 1 with improved growth character. The cross breeding method comprises the following steps: 1, selection of rapidly-growing cross parents: 1.1, selection of a female parent, and 1.2, selection of a male parent; 2, strengthening breeding of parents; 3, artificial parturition hastening; 4, artificial cross fertilization; 5, artificial incubation; and 6, fry breeding. The cross breeding method provided by the invention breeds the first-filial generation of Huangyou No. 1 by cross breeding of female Pelteobagrus fulvidraco and pelteobagrus vachelli; and the first-filial generation of Huangyou No. 1 is tawny in body color, and has extremely significant growth vigor and strong stress resistance.
Description
Technical field
The invention belongs to technical field of aquaculture, and in particular to a kind of yellow excellent No. 1 Pelteobagrus fulvidraco of improvement growth traits
Cross breeding method, is a kind of using fish interspecific hybridization breeding technique, parental line selection and to cultivate innovative technology and produce and have life
Yellow excellent No. 1 Pelteobagrus fulvidraco of new varieties of quick, the degeneration-resistant and disease-resistant advantage of length.
Background technology
Pelteobagrus fulvidraco Pelteobagrus fulvidraco (Richardson) is subordinate to SILURIFORMES (Sliuriformes), Chang
Section (Bagridae), Pelteobagrus (Pelteobagrus), popular name spotted maigre, yellow loud, high-pitched sound, yellow cured fourth etc..Pelteobagrus vachelli
Pelteobagrus vachehi (Richardson) are subordinate to SILURIFORMES (Sliuriformes), Chang sections (Bagridae), yellow forehead
Fish belongs to (Pelteobagrus), popular name youth's silk, Huang Lading, river forehead etc..Pelteobagrus fulvidraco Pelteobagrus fulvidraco and Wa Shi
Pelteobagrus fulvidraco Pelteobagrus vachehi are widely distributed to belong to fish together in ChangJiang River, are that the more important bottom of China is little
Type economic fish.Pelteobagrus fulvidraco is suitable to be cultivated in pond, but its individuality is less, and the speed of growth is slower, and Pelteobagrus vachelli is individual
Greatly, grow rapidly, but its hypoxia-resistant capacity and resistance are poor, it is more difficult to it is suitable to pond culture.Additionally, the most body of Pelteobagrus vachelli
Color is in canescence, is traditionally not easy to receive in the market comsupton of most area.
In recent years, domestic Yellow cartfish adult cultured output is more than 400,000 tons, more than 80 hundred million yuan of annual value of production.But with yellow forehead
The fast development of fish culture industry, some problems occur in succession, and for example, inbred phenomenon is serious, cause Pelteobagrus fulvidraco germplasm to be degenerated,
The speed of growth is reduced, premunition declines, and disease is on the rise.In seed rearing stage parasite, bacterial gill rot disease, Beancurd sheet
Normal cultivation with the disease such as enteritis has had a strong impact on Pelteobagrus fulvidraco seed, makes Pelteobagrus fulvidraco seed form rate relatively low.Every year due to disease
Evil causes the economic loss of Pelteobagrus fulvidraco aquaculture heavy, and the cultivation for having had a strong impact on Pelteobagrus fulvidraco is promoted.Grow slack-off and disease
It is the bottleneck factor for limiting China's Pelteobagrus fulvidraco aquaculture sustainable development to take place frequently, and also have impact on Pelteobagrus fulvidraco entering in coastal provinces and cities
One step is promoted and cultivated.Therefore, the development of Pelteobagrus fulvidraco aquaculture is strong in the urgent need to cultivating quick growth, resistance and disease resistance
Improved Varieties.
Hybridization refers between variety classes or carries out mating between same kind of Different population cultivates the side of offspring
Method.Because two parents differ farther out on genetic distance, thus the offspring of hybridization, that is, hybridize first filial generation in growth performance and resist
The aspects such as disease are typically superior to parents, and this phenomenon is referred to as heterosis, hybrid vigor.Crossbreeding is most important in Crop Genetic Breeding
One of method, so far, cultivates some and has on growth performance by variety classes hybridization in some cereal crops
The Hybrid having a clear superiority, such as hybrid rice and corn etc..It is domestic in recent years in terms of Pelteobagrus fulvidraco genetic breeding research,
Genetically engineered cell line and fish sex control breeding technique (Liu Hanqin etc. 2007 are mainly carried out；Xu Hui, 2007；Chen Rongguo etc.,
2013).But carry out hybridization cultivation with the Pelteobagrus fulvidraco of strong stress resistance and Pelteobagrus vachelli about screening and cultivation growth to grow and anti-
The strong improved Varieties of sick power, have not yet to see report.
The content of the invention
To solve the problems, such as above-mentioned prior art, the invention provides a kind of yellow excellent No. 1 Huang of improvement growth traits
The cross breeding method of forehead fish, it is yellow with first-filial generation F1 of Pelteobagrus vachelli ♂ crossbreeds that the method has cultivated Pelteobagrus fulvidraco ♀
Excellent No. 1, its body colour is yellowish-brown, and growth vigor is extremely obvious, strong stress resistance.
Realize technical scheme that above-mentioned purpose of the present invention adopted for：
A kind of cross breeding method of yellow excellent No. 1 Pelteobagrus fulvidraco of improvement growth traits, comprises the steps：
1st, the screening of fast-growth hybrid strain：
1.1st, maternal screening is hybridized：
Mid-April in the first year to by the end of November, select body surface without wound, body weight more than 100g wild female Pelteobagrus fulvidraco as first
Beginning Pelteobagrus fulvidraco is maternal；Select body surface without wound, body weight more than 250g wild males Pelteobagrus fulvidraco as initial Pelteobagrus fulvidraco male parent, just
After the maternal and initial Pelteobagrus fulvidraco male parent of beginning Pelteobagrus fulvidraco chooses, under the conditions of propagating artificially, Jing tame and docile food can ingest hand fit feeding
Initial Pelteobagrus fulvidraco parent culture is carried out after material, in 5-7 months after year, the gonad development degree of initial Pelteobagrus fulvidraco parent population is observed, is selected
The initial Pelteobagrus fulvidraco parent of fully-developed carries out breeding and hastens parturition, hatches and nurture of fish fingerlings, closes in Pelteobagrus fulvidraco seed fast-growth
The key stage, i.e., 7-9 months then, growth selection is fast, the individuality of high-output stress-resistance, and the individuality of the 4-6% that selects and remain is cultivated, the
Sexal maturity is reached within 2 years or the 3rd year, selects female as fast-growth hybridization Pelteobagrus fulvidraco female parent breeding line, referred to as hybridized maternal；
1.2nd, the screening of parent sire of hybrid pigs：
Mid-April in the first year, selection body surface was made without wound, body weight in the wild female Pelteobagrus vachelli of more than 300g to by the end of November
It is maternal for initial Pelteobagrus vachelli；Select body surface without wound, body weight more than 500g wild males Pelteobagrus vachelli as initial
Pelteobagrus vachelli male parent, after the maternal and initial Pelteobagrus vachelli male parent of initial Pelteobagrus vachelli chooses, is propagating condition artificially
Under, initial Pelteobagrus vachelli parent culture is carried out Jing after tame and docile food can ingest artifical compound feed, 5-7 months after year, observation is initial
The gonad development degree of Pelteobagrus vachelli parent population, selects the initial Pelteobagrus vachelli parent of fully-developed to carry out breeding and hastens parturition, incubates
Change and nurture of fish fingerlings, in Pelteobagrus vachelli seed fast-growth critical stage, i.e., 7-9 month growth selections then are very fast,
The individuality of high-output stress-resistance, the individuality of the 1-2% that selects and remain is cultivated, and reaches within the 3rd year sexal maturity, selects male as fast fast-growing
Long hybridization Pelteobagrus fulvidraco male parent breeding line, abbreviation parent sire of hybrid pigs；
2nd, the reinforced cultivating of hybrid strain：
2.1st, maternal reinforced cultivating is hybridized：
The well-developed hybridization female parent of sexual gland is selected to render in the A of reinforced cultivating pond, breeding density is 260-300 pound/acre,
Wait hybridize it is maternal adapt to reinforced cultivating pond nurturing an environment after, start domestication and ingest special parent's mixed feed, day feeding volume is miscellaneous
Hand over the 1-1.5% of maternal gross weight, feed daily 2 times, Feeding time be at 6 points in the morning to 6 thirty, 6 pm half to 7 points, on
The feeding volume in noon and afternoon accounts for respectively the 30-50% and 50-70% of day feeding volume, 1 month before artificial induced spawning, matches somebody with somebody in special parent
Close the hybridization female parent that the fly maggot slurry that addition quality proportion in feed is special parent's mixed feed quality 3%-5% is carried out 2 weeks
Reinforced cultivating, while being sampled inspection to hybridizing female parent every 1 week, checks the developmental state of the maternal sexual gland of hybridization；
2.2nd, the reinforced cultivating of parent sire of hybrid pigs：
The well-developed parent sire of hybrid pigs of sexual gland is selected to render in the B of reinforced cultivating pond, breeding density is 260-300 pound/acre,
After parent sire of hybrid pigs adapts to reinforced cultivating pond nurturing an environment, start domestication and ingest special parent's mixed feed, day feeding volume is miscellaneous
Hand over the 1-1.5% of male parent gross weight, feed daily 2 times, Feeding time be at 6 points in the morning to 6 thirty, 6 pm half to 7 points, on
The feeding volume in noon and afternoon accounts for respectively the 30-50% and 50-70% of day feeding volume；
3rd, artificial induced spawning：
After treating hybridization female parent and parent sire of hybrid pigs gonadal maturation, maternal and parent sire of hybrid pigs will be hybridized and thrown in respectively into two
Separate in individual different spawning inducing pool and temporarily support, maternal and parent sire of hybrid pigs the input ratio of hybridization is calculated as 80-100 by tail：1, two urge
Produce water temperature and be 25-30 DEG C, it is 10-15kg/m to hybridize maternal breeding density2Water body, during hastening parturition, spawning inducing pool is in stream
Water environment, flowing water flow is 0.5-1.0m3/ h, after maternal second injection of exogenous hormone is hybridized, increases flowing water flow, increases
Plus flowing water flow is to 2.0-3.0m3/h；
Hybrid strain is temporarily supported and proceeds by artificial induced spawning after spawning inducing pool 3-5 hour, in hybridization female parent and parent sire of hybrid pigs abdomen
Chamber pectoral fin base portion injection of exogenous hormone, hybridization is maternal to inject exogenous hormones twice, and twice time interval 10-12 hour, miscellaneous
When handing over maternal first time injection of exogenous hormone, exogenous hormones is promoted by fish luteotropin releasing hormone d-ala analog and chorion
Gonadal hormone is constituted, and its each component consumption is as follows：Fish luteotropin releasing hormone d-ala analog 10-16ug/kg fish bodies, chorion
Promoting sexual gland hormone 100-200IU/kg fish bodies；When hybridizing maternal second injection of exogenous hormone, exogenous hormones is promoted by fish
Luteinizing hormone releasing hormone analog, human chorionic gonadtropin and maleic acid DOM composition, its each component consumption is as follows：Fish is used
Luteotropin releasing hormone d-ala analog 16-20ug/kg fish bodies, human chorionic gonadtropin 1200-2000IU/kg fish bodies, Malaysia
Sour DOM 4-8mg/kg fish bodies；Parent sire of hybrid pigs injects an exogenous hormones, and the exogenous hormones of parent sire of hybrid pigs injection is by fish
Constituted with luteotropin releasing hormone d-ala analog, human chorionic gonadtropin and maleic acid DOM, its each component consumption is as follows：
Fish luteotropin releasing hormone d-ala analog 5-8ug/kg fish bodies, human chorionic gonadtropin 600-1000IU/kg fish bodies, Malaysia
Sour DOM 2.0-4.0mg/kg fish bodies；
4th, artificial hybridization fertilization
After hybridizing maternal second injection of exogenous hormone 8-12 hours, every half an hour sampling Detection maternal sexual gland is hybridized
Ovulation situation, when the individuality of 60-70% is attained by smoothly completing manually crowded ovum, starts manually crowded ovum and receives ovum, collects every time
After 80-100 tail raun fish-eggs, the seminal fluid of the 1 tail parent sire of hybrid pigs for preparing in advance is added in the fish-egg of collection to be carried out manually
Insemination operation；
5th, artificial incubation：
To be hatched in embryonated egg incubator disposed within, hatching density is ten thousand embryonated eggs of 60-70/m3Water body, hatching
Flowing water environment is kept in case, makes embryonated egg in flowing water environment, flow velocity is 0.2-0.3m/s；
Embryonated egg starts 4-8 hours after demoulding, is in batches dragged for embryonated egg into stainless steel washbowl with screen cloth, using normal
The density of development demoulding embryonated egg weeds out dead ovum and mouldy fertilization higher than dead ovum and this characteristic of mouldy embryonated egg
Ovum, the embryonated egg of normal development is transferred in other off-the-shelf incubator and proceeds hatching；
6th, the cultivation of seed：
After in incubator more than 95% embryonated egg demoulding 12 hours, hybridization yolk seedling is thrown in cement pit and carried out
Temporarily support and cultivate, breeding density is ten thousand tails of 5-10/m2Water body, yolk seedling starts appearance and climbs wall phenomenon on a small quantity after temporarily supporting 2-3 days, now
Yolk seedling is sold or thrown in carries out seed rearing into off-the-shelf special pond, breeding density be the tails of 20-25 ten thousand/
Mu, hybrid seedlings are Jing after cultivating, you can obtain yellow excellent No. 1 Pelteobagrus fulvidraco of big specification.
Further, the maternal and initial Pelteobagrus fulvidraco male parent of described initial Pelteobagrus fulvidraco is collection from the lake of the Yangtze river basin, institute
The lake stated is Liangzi Lake, Honghu, Dongting Lake or Poyang Lake, described wild Pelteobagrus vachelli from Yangtze river basin Yueyang section extremely
Wuhan section.
Further, described spawning inducing pool depth of water 0.5-0.6m.
Further, embryonated egg filters bolting silk net once during incubator hatching every 1-2h cleanings, and embryonated egg starts to take off
Bolting silk net is filtered after film once every 0.5h cleanings.
Further, the long 5-10m of described cement pit, width 1.5-2.0m, high 0.8-1.2m, depth of water 0.4-0.6m is described
Special pond area 2-4 mus, depth of water 0.6-1.0m.
Compared with prior art, its advantage and advantage are the present invention：
1. the present invention takes the lead in using technologies such as unexpected mass incident, interspecific hybridization, cultivates the speed of growth and is substantially better than all-male Huang
Forehead fish and common Pelteobagrus fulvidraco, the Pelteobagrus fulvidraco new varieties of degeneration-resistant and disease-resistant improvement are yellow excellent No. 1, and the growth rate that Huang You 1 is than common
Pelteobagrus fulvidraco improves more than 35.66%, and than all-male Pelteobagrus fulvidraco more than 11.94% is improved.
2. parent's cultivation method for enhancing that the present invention sets up, with simple to operate, management easily, efficiency high, effect it is obvious
The features such as, it is ensured that the synchronous sexal maturity of parent, prolongation can breed the time, can carry out within 1 year 2-3 time and hasten parturition to breed, and greatly shorten
The time that Pelteobagrus fulvidraco single is bred, efficiency is bred so as to substantially increase Pelteobagrus fulvidraco, at aspects such as Pelteobagrus fulvidraco parent cultures, solely
Carried out invasively limit food feed process and add in parent culture feed fly maggot slurry Pelteobagrus fulvidraco parent is carried out it is effectively strong
Change and cultivate and successful, more than 95%, the maternal sexual gland proportion of hybridization reaches 13-15% to parent's sexal maturity.
3. the cross breeding method of the yellow excellent No. 1 yellow catfish growing proterties that the present invention sets up is the training of fish high-yield anti-adversity
Educate and open new technological approaches, excellent No. 1 yellow to fully exploitation hybridization Pelteobagrus fulvidraco creates as famous-particular-excellent breed variety and most has
The Practice Measures and means of effect, with great Social and economic benef@.
Specific embodiment
With reference to specific embodiment, the present invention is described in detail.
Embodiment 1
1st, the screening of fast-growth hybrid strain：
1.1st, maternal screening：
In mid-April, 2010, collection came from the lake of Yangtze river basin periphery (such as Liangzi Lake, Honghu, Dongting Lake to by the end of November
Lake and Poyang Lake) wild Pelteobagrus fulvidraco, select body surface without wound, body weight more than 100g healthy and strong wild female Pelteobagrus fulvidraco as first
Beginning Pelteobagrus fulvidraco is maternal；Select body surface without wound, body weight more than 250g healthy and strong wild males Pelteobagrus fulvidraco as initial Pelteobagrus fulvidraco father
This, after the maternal and initial Pelteobagrus fulvidraco male parent of initial Pelteobagrus fulvidraco chooses, under the conditions of propagating artificially, Jing tames and dociles food and can ingest and manually matches somebody with somebody
Closing carries out initial Pelteobagrus fulvidraco parent culture after feed, 5-7 months in 2011, observe the gonad development journey of initial Pelteobagrus fulvidraco parent population
Degree, selects fully-developed Pelteobagrus fulvidraco parent to carry out breeding and hastens parturition, hatches and nurture of fish fingerlings, in Pelteobagrus fulvidraco seed fast-growth
Critical stage (the long 5-15cm of body), i.e., the 7-9 months of 2011, growth selection is fast, the individuality of high-output stress-resistance, selects and remain about 5%
Individuality cultivated, until 3-4 months in 2012, most of individuality attainability is ripe, selects female as fast-growth
Hybridization Pelteobagrus fulvidraco female parent (A systems), referred to as hybridizes maternal；
1.2nd, the screening of male parent：
In mid-April, 2009, to by the end of October, collects the Pelteobagrus vachelli from Yangtze river basin Yueyang section to Wuhan section, selects
Body surface is maternal as initial Pelteobagrus vachelli in the healthy and strong wild female Pelteobagrus vachelli of more than 300g without wound, body weight；Selective body
Table without wound, body weight more than 500g healthy and strong wild males Pelteobagrus vachelli as initial Pelteobagrus vachelli male parent, initial Wa Shi
After the maternal and initial Pelteobagrus vachelli male parent of Pelteobagrus fulvidraco chooses, under the conditions of propagating artificially, Jing tames and dociles food and can ingest hand fit
Initial Pelteobagrus vachelli parent culture is carried out after feed, 5-7 months in 2010, the sexual gland for observing initial Pelteobagrus vachelli parent population is sent out
Degree is educated, is selected the initial Pelteobagrus vachelli parent of fully-developed to carry out breeding and is hastened parturition, hatches and nurture of fish fingerlings, it is yellow in Wa Shi
Forehead fry kind fast-growth critical stage (the long 10-25cm of body), i.e., the 7-9 months of 2010, growth selection is very fast, anti-adversity ability
Strong individuality, about 1% individuality selected and remain is cultivated, to 3-4 months in 2012 or so, most of individuality reaches sexal maturity, choosing
Male is selected as fast-growth hybridization Pelteobagrus fulvidraco male parent (B systems), abbreviation parent sire of hybrid pigs；
2nd, the reinforced cultivating of hybrid strain：
2.1st, maternal reinforced cultivating is hybridized：
The 3-4 months of 2012, select the well-developed hybridization female parent of sexual gland to render in the A of reinforced cultivating pond, put close in a suitable place to breed
Spend for 280 pounds/acre, wait hybridize it is maternal adapt to reinforced cultivating pond nurturing an environment after, start domestication and ingest special parent's mixed feed
(commercially available), day feeding volume is the 1-1.5% of the maternal gross weight of hybridization, is fed daily 2 times, and Feeding time is 6 points to 6 points of the morning
The feeding volume in the halfth, half to 7 points of 6 pm, the morning and afternoon accounts for respectively the 40% and 60% of day feeding volume, 1 before artificial induced spawning
Month, the fly maggot slurry that quality proportion is special parent's mixed feed quality 5% is added in special parent's mixed feed carries out 2 weeks
The maternal reinforced cultivating of hybridization, while being sampled inspection to hybridizing female parent every 1 week, checks the development feelings of the maternal sexual gland of hybridization
Condition；
2.2nd, the reinforced cultivating of parent sire of hybrid pigs：
The 3-4 months of 2012, select the well-developed parent sire of hybrid pigs of sexual gland to render in the B of reinforced cultivating pond, put close in a suitable place to breed
Spend for 280 pounds/acre, after parent sire of hybrid pigs adapts to reinforced cultivating pond nurturing an environment, start domestication and ingest special parent's mixed feed
(commercially available), day feeding volume is the 1-1.5% of parent sire of hybrid pigs gross weight, feeds daily 2 times, and Feeding time is 6 points to 6 points of the morning
The feeding volume in the halfth, half to 7 points of 6 pm, the morning and afternoon accounts for respectively the 40% and 60% of day feeding volume；
3rd, artificial induced spawning：
In May, 2012, after treating hybridization female parent and parent sire of hybrid pigs gonadal maturation, maternal and parent sire of hybrid pigs will be hybridized and divided
Do not throw in separate in two different spawning inducing pools and temporarily support, maternal and parent sire of hybrid pigs the input ratio of hybridization is calculated as 90 by tail：
1, the two spawning inducing pool depth of waters are 0.5-0.6m, and two spawning inducing pool water temperatures are 25-30 DEG C, hybridize maternal breeding density and are
12kg/m2Water body, during hastening parturition, spawning inducing pool is in flowing water environment, and flowing water flow is 0.5m3/ h, treats that hybridization is injected for maternal second
After exogenous hormones, flowing water flow is increased, increase flowing water flow to 2.0m3/h；
Hybrid strain is temporarily supported and proceeds by artificial induced spawning after spawning inducing pool 3-5 hour, in hybridization female parent and parent sire of hybrid pigs abdomen
Chamber pectoral fin base portion injection of exogenous hormone, hybridization is maternal to inject exogenous hormones twice, twice time interval 11 hours, and hybridization is female
During this first time injection of exogenous hormone, exogenous hormones is by fish luteotropin releasing hormone d-ala analog and chorionic gonadotrophin
Hormone is constituted, and its each component consumption is as follows：Fish luteotropin releasing hormone d-ala analog 12ug/kg fish bodies, chorionic gonadotrophin
Hormone 175IU/kg fish bodies, when hybridizing maternal second injection of exogenous hormone, exogenous hormones is discharged by fish with luteotropin
Hormone analogs, human chorionic gonadtropin and maleic acid DOM composition, its each component consumption is as follows：Fish is released with luteotropin
Hormone analogs 18ug/kg fish bodies, human chorionic gonadtropin 1750IU/kg fish bodies, maleic acid DOM 7mg/kg fish bodies are put,
Parent sire of hybrid pigs injects an exogenous hormones, and the exogenous hormones of parent sire of hybrid pigs injection is similar to by fish with luteinizing hormone releasing hormone
Thing, human chorionic gonadtropin and maleic acid DOM composition, its each component consumption is as follows：Fish luteinizing hormone releasing hormone class
Like thing 6ug/kg fish bodies, human chorionic gonadtropin 900IU/kg fish bodies, maleic acid DOM 3.0mg/kg fish bodies.
4th, artificial hybridization fertilization
Hybridize maternal second injection of exogenous hormone after 10 hours, every half an hour sampling Detection maternal sexual gland row is hybridized
Ovate condition, when the individuality of 60-70% is attained by smoothly completing manually crowded ovum, you can start manually crowded ovum and receive ovum, receive every time
Collect 90 tail raun fish-eggs, you can the seminal fluid of 1 tail parent sire of hybrid pigs for starting to prepare in advance is added in fish-egg manually to be awarded
Essence operation.
5th, artificial incubation：
To be hatched in embryonated egg incubator disposed within, hatching density is ten thousand embryonated eggs of 60-70/m3Water body, hatching
Flowing water environment is kept in case, makes embryonated egg in flowing water environment, flow velocity is 0.25m/s, adjusted current and ensure that embryonated egg is firm
Rush to water surface well；
Embryonated egg starts 4-8 hours after demoulding, is in batches dragged for embryonated egg to stainless steel washbowl (diameter with the screen cloth of 80 mesh
It is higher than this characteristic of dead ovum and mouldy embryonated egg using the density of the embryonated egg of normal development demoulding in 40cm), weeds out
Dead ovum and mouldy embryonated egg, the embryonated egg of normal development is transferred in other off-the-shelf incubator proceed afterwards
Hatching, can prevent normal fertilization ovum by mycotic infection, and conspicuousness improves the incubation rate of embryonated egg.
6th, the cultivation of seed：
After in incubator more than 95% embryonated egg demoulding 12 hours, yolk seedling is thrown in and carry out in cement pit temporarily
Culture, the long 10m of cement pit, width 1.5m, high 0.8m, depth of water 0.4-0.6m, breeding density is ten thousand tails of 5-10/m2Water body, yolk seedling
Start appearance after temporarily supporting 2-3 days and climb wall phenomenon on a small quantity, now yolk seedling is sold or thrown in carries out seed into special pond
Cultivation, 4 mu of special pond area, depth of water 0.6-1.0m, breeding density be ten thousand tails of 20-25/mu, hybrid seedlings Jing cultivate after,
Obtain yellow excellent No. 1 Pelteobagrus fulvidraco of big specification.
The growth performance and anti-infection ability evaluation and test experiment of yellow excellent No. 1 Pelteobagrus fulvidraco that experiment one, the present embodiment is bred
Experimental technique：
1st, when excellent No. 1 Pelteobagrus fulvidraco of Huang, all-male Pelteobagrus fulvidraco and common yellow catfish growing are to 5-8cm, No. 1 Huang of Huang You is taken at random
The each 400-500 tails of forehead fish, all-male Pelteobagrus fulvidraco and common Pelteobagrus fulvidraco, select neat specification, body surface without wound, physique healthy and strong it is yellow excellent
Each 90 tail of No. 1 Pelteobagrus fulvidraco, all-male Pelteobagrus fulvidraco and common Pelteobagrus fulvidraco, measures its average body length and body weight, as initial when putting in a suitable place to breed
Body length and body weight, then by yellow excellent No. 1 Pelteobagrus fulvidraco of 90 tails of select, all-male Pelteobagrus fulvidraco and common Pelteobagrus fulvidraco is each puts in a suitable place to breed at random 3
In individual glass fibre culturing jar (270L), per 30, cylinder, notes adjusting the weight of often nautilus between each cylinder without significant difference.Support
Sampled measurements are carried out to the body length and body weight of excellent No. 1 Pelteobagrus fulvidraco of Huang, all-male Pelteobagrus fulvidraco and common Pelteobagrus fulvidraco after growing 8 weeks, calculates yellow
Excellent No. 1 Pelteobagrus fulvidraco, all-male Pelteobagrus fulvidraco, the body length of common Pelteobagrus fulvidraco and the mean value of body weight, are increased using specific growth rate and body weight
Again rate to the growth performance of excellent No. 1 Pelteobagrus fulvidraco, all-male Pelteobagrus fulvidraco and common Pelteobagrus fulvidraco being compared evaluation.
2nd, when excellent No. 1 Pelteobagrus fulvidraco of Huang, all-male Pelteobagrus fulvidraco and common yellow catfish growing are to 5-8cm, No. 1 Huang of Huang You is taken at random
The each 400-500 tails fry of forehead fish, all-male Pelteobagrus fulvidraco and common Pelteobagrus fulvidraco, selects neat specification, body surface without wound, physique healthy and strong
Yellow excellent No. 1 Pelteobagrus fulvidraco, all-male Pelteobagrus fulvidraco and each 180 tail of common Pelteobagrus fulvidraco, it is each to put in a suitable place to breed at random in 6 glass fibre culturing jars
(270L) in, per 30, cylinder.Cultured cause of disease Aeromonas hydrophila (Aeromonas hydrophila) is used into physiological saline
Concentration is diluted to for 1 × 106The bacterium solution of CFU/ml, in pectoral fin base portion bacterium solution 0.005ml/g fish body weight is injected, and challenge test respectively sets
One control group (physiological saline of injection same dose), 3 parallel, and experimental period is 1 week, and daily observed and recorded experiment fish is dead
Situation.The survival rate that each group tests fish is calculated, using survival rate come to excellent No. 1 Pelteobagrus fulvidraco of Huang, all-male Pelteobagrus fulvidraco and common Huang forehead
The anti-infection ability of fish is compared evaluation.
Experimental result：
Excellent No. 1 Pelteobagrus fulvidraco, all-male Pelteobagrus fulvidraco, the growth performance of common Pelteobagrus fulvidraco and anti-infection ability evaluation result such as following table
Shown in 1：
Yellow excellent No. 1, the all-male Pelteobagrus fulvidraco of table 1 and common yellow catfish growing performance and anti-infection ability determine
Note：Numerical value (mean+SD) is the mean value of 3 repetitions in table, and Superscript letters different after numerical value are represented
Significant difference (P<0.05).
Embodiment 2
1st, the screening of fast-growth hybrid strain：
1.1st, maternal screening：
In by the end of September, 2012 to by the end of November, collection comes from the lake of Yangtze river basin periphery (such as Liangzi Lake, Honghu, Dongting Lake
And Poyang Lake) wild Pelteobagrus fulvidraco, select body surface without wound, healthy and strong wild female Pelteobagrus fulvidraco of the body weight more than 100 be used as initial
Pelteobagrus fulvidraco is maternal；Select body surface without wound, body weight more than 250g healthy and strong wild males Pelteobagrus fulvidraco as initial Pelteobagrus fulvidraco male parent,
After the initial maternal and initial Pelteobagrus fulvidraco male parent of Pelteobagrus fulvidraco chooses, under the conditions of propagating artificially, the tame and docile foods of Jing can ingest hand fit
Initial Pelteobagrus fulvidraco parent culture is carried out after feed, 5-7 months in 2013, the gonad development degree of initial Pelteobagrus fulvidraco parent population is observed,
Select fully-developed Pelteobagrus fulvidraco parent to carry out breeding to hasten parturition, hatch and nurture of fish fingerlings, close in Pelteobagrus fulvidraco seed fast-growth
The key stage (the long 5-15cm of body), i.e., the 7-9 months of 2013, growth selection is fast, the individuality of high-output stress-resistance, selects and remain about 4%
Individuality is cultivated, until 3-4 months in 2014, most of individuality attainability maturation, select female miscellaneous as fast-growth
Pelteobagrus fulvidraco female parent (A systems) is handed over, referred to as hybridizes maternal；
1.2nd, the screening of male parent：
Collect the Pelteobagrus vachelli from Yangtze river basin Yueyang section to Wuhan section, selective body to by the end of November in by the end of September, 2011
Table is maternal as initial Pelteobagrus vachelli in the healthy and strong wild female Pelteobagrus vachelli of more than 300g without wound, body weight；Select body surface
Without wound, body weight more than 500g healthy and strong wild males Pelteobagrus vachelli as initial Pelteobagrus vachelli male parent, initial Wa Shi is yellow
After the maternal and initial Pelteobagrus vachelli male parent of forehead fish chooses, under the conditions of propagating artificially, Jing tame and docile food can ingest hand fit feeding
Initial Pelteobagrus vachelli parent culture is carried out after material, 5-7 months in 2012, the gonad development of initial Pelteobagrus vachelli parent population is observed
Degree, selects the initial Pelteobagrus vachelli parent of fully-developed to carry out breeding and hastens parturition, hatches and nurture of fish fingerlings, in Wa Shi Huang foreheads
Fry kind fast-growth critical stage (the long 10-25cm of body), i.e., in 2012 7-9 months, growth selection is very fast, high-output stress-resistance
Individuality, about 1% individuality selected and remain is cultivated, to 3-4 months in 2014 or so, most of individuality reaches sexal maturity, selects male
Property as fast-growth hybridization Pelteobagrus fulvidraco male parent (B systems), abbreviation parent sire of hybrid pigs；
2nd, the reinforced cultivating of hybrid strain：
2.1st, maternal reinforced cultivating is hybridized：
The 3-4 months of 2014, select the well-developed hybridization female parent of sexual gland to render in the A of reinforced cultivating pond, put close in a suitable place to breed
Spend for 260 pounds/acre, wait hybridize it is maternal adapt to reinforced cultivating pond nurturing an environment after, start domestication and ingest special parent's mixed feed
(commercially available), day feeding volume is the 1-1.5% of the maternal gross weight of hybridization, is fed daily 2 times, and Feeding time is 6 points to 6 points of the morning
The feeding volume in the halfth, half to 7 points of 6 pm, the morning and afternoon accounts for respectively the 40% and 60% of day feeding volume, 1 before artificial induced spawning
Month, the fly maggot slurry that quality proportion is special parent's mixed feed quality 4% is added in special parent's mixed feed carries out 2 weeks
The maternal reinforced cultivating of hybridization, while being sampled inspection to hybridizing female parent every 1 week, checks the development feelings of the maternal sexual gland of hybridization
Condition；
2.2nd, the reinforced cultivating of parent sire of hybrid pigs：
The 3-4 months of 2014, select the well-developed parent sire of hybrid pigs of sexual gland to render in the B of reinforced cultivating pond, put close in a suitable place to breed
Spend for 260 pounds/acre, after parent sire of hybrid pigs adapts to reinforced cultivating pond nurturing an environment, start domestication and ingest special parent's mixed feed
(commercially available), day feeding volume is the 1-1.5% of parent sire of hybrid pigs gross weight, feeds daily 2 times, and Feeding time is 6 points to 6 points of the morning
The feeding volume in the halfth, half to 7 points of 6 pm, the morning and afternoon accounts for respectively the 40% and 60% of day feeding volume；
3rd, artificial induced spawning：
In May, 2014, after treating hybridization female parent and parent sire of hybrid pigs gonadal maturation, maternal and parent sire of hybrid pigs will be hybridized and divided
Do not throw in separate in two different spawning inducing pools and temporarily support, maternal and parent sire of hybrid pigs the input ratio of hybridization is calculated as 80 by tail：
1, two spawning inducing pool water temperatures are 25-30 DEG C, and two spawning inducing pool depth of waters are 0.5-0.6m, hybridize maternal breeding density and are
15kg/m2Water body, during hastening parturition, spawning inducing pool is in flowing water environment, and flowing water flow is 0.7m3/ h, treats that hybridization is injected for maternal second
After exogenous hormones, flowing water flow is increased, increase flowing water flow to 2.5m3/h；
Hybrid strain is temporarily supported and proceeds by artificial induced spawning after spawning inducing pool 3-5 hour, in hybridization female parent and parent sire of hybrid pigs abdomen
Chamber pectoral fin base portion injection of exogenous hormone, hybridization is maternal to inject exogenous hormones twice, twice time interval 10 hours, and hybridization is female
During this first time injection of exogenous hormone, exogenous hormones is by fish luteotropin releasing hormone d-ala analog and chorionic gonadotrophin
Hormone is constituted, and its each component consumption is as follows：Fish luteotropin releasing hormone d-ala analog 16ug/kg fish bodies, chorionic gonadotrophin
Hormone 200IU/kg fish bodies, when hybridizing maternal second injection of exogenous hormone, exogenous hormones is discharged by fish with luteotropin
Hormone analogs, human chorionic gonadtropin and maleic acid DOM composition, its each component consumption is as follows：Fish is released with luteotropin
Hormone analogs 16ug/kg fish bodies, human chorionic gonadtropin 1200IU/kg fish bodies, maleic acid DOM 8mg/kg fish bodies are put,
Parent sire of hybrid pigs injects an exogenous hormones, and the exogenous hormones of parent sire of hybrid pigs injection is similar to by fish with luteinizing hormone releasing hormone
Thing, human chorionic gonadtropin and maleic acid DOM composition, its each component consumption is as follows：Fish luteinizing hormone releasing hormone class
Like thing 8ug/kg fish bodies, human chorionic gonadtropin 1000IU/kg fish bodies, maleic acid DOM 2.0mg/kg fish bodies.
4th, artificial hybridization fertilization
Hybridize maternal second injection of exogenous hormone after 8 hours, every half an hour sampling Detection maternal sexual gland row is hybridized
Ovate condition, when the individuality of 60-70% is attained by smoothly completing manually crowded ovum, you can start manually crowded ovum and receive ovum, receive every time
Intensive 80 tail raun fish-egg, you can the seminal fluid of the 1 tail hybridization father and mother for starting to prepare in advance be added in fish-egg carry out it is artificial
Insemination operation.
5th, artificial incubation：
To be hatched in embryonated egg incubator disposed within, hatching density is ten thousand embryonated eggs of 60-70/m3Water body, hatching
Flowing water environment is kept in case, makes embryonated egg in flowing water environment, flow velocity is 0.20m/s, adjusted current and ensure that embryonated egg is firm
Rush to water surface well；
Embryonated egg starts 4-8 hours after demoulding, is in batches dragged for embryonated egg to stainless steel washbowl (diameter with the screen cloth of 80 mesh
In 40cm), this characteristic of dead ovum and mouldy embryonated egg is higher than using the density of the embryonated egg of normal development demoulding, is rejected
Fall dead ovum and mouldy embryonated egg, the embryonated egg of normal development is transferred in other off-the-shelf incubator afterwards continue into
Row hatching, can prevent normal fertilization ovum by mycotic infection, and conspicuousness improves the incubation rate of embryonated egg.
6th, the cultivation of seed：
After in incubator more than 95% embryonated egg demoulding 12 hours, yolk seedling is thrown in and carry out in cement pit temporarily
Culture, the long 10m of cement pit, width 1.5m, high 0.8m, depth of water 0.4-0.6m, breeding density is ten thousand tails of 5-10/m2Water body, yolk seedling
Start appearance after temporarily supporting 2-3 days and climb wall phenomenon on a small quantity, now yolk seedling is sold or thrown in carries out seed into special pond
Cultivation, 3 mu of special pond area, depth of water 0.6-1.0m, breeding density be ten thousand tails of 20-25/mu, hybrid seedlings Jing cultivate after,
Obtain yellow excellent No. 1 Pelteobagrus fulvidraco of big specification.
The growth performance and anti-infection ability evaluation and test experiment of yellow excellent No. 1 Pelteobagrus fulvidraco that experiment two, the present embodiment is bred
Experimental technique：
1st, when excellent No. 1 Pelteobagrus fulvidraco of Huang, all-male Pelteobagrus fulvidraco and common yellow catfish growing are to 5-8cm, No. 1 Huang of Huang You is taken at random
The each 400-500 tails of forehead fish, all-male Pelteobagrus fulvidraco and common Pelteobagrus fulvidraco, select neat specification, body surface without wound, physique healthy and strong it is yellow excellent
Each 90 tail of No. 1 Pelteobagrus fulvidraco, all-male Pelteobagrus fulvidraco and common Pelteobagrus fulvidraco, measures its average body length and body weight, as initial when putting in a suitable place to breed
Body length and body weight, then by yellow excellent No. 1 Pelteobagrus fulvidraco of 90 tails of select, all-male Pelteobagrus fulvidraco and common Pelteobagrus fulvidraco is each puts in a suitable place to breed at random 3
In individual glass fibre culturing jar (270L), per 30, cylinder, notes adjusting the weight of often nautilus between each cylinder without significant difference.Support
Sampled measurements are carried out to the body length and body weight of excellent No. 1 Pelteobagrus fulvidraco of Huang, all-male Pelteobagrus fulvidraco and common Pelteobagrus fulvidraco after growing 8 weeks, calculates yellow
Excellent No. 1 Pelteobagrus fulvidraco, all-male Pelteobagrus fulvidraco, the body length of common Pelteobagrus fulvidraco and the mean value of body weight, are increased using specific growth rate and body weight
Again rate to the growth performance of excellent No. 1 Pelteobagrus fulvidraco, all-male Pelteobagrus fulvidraco and common Pelteobagrus fulvidraco being compared evaluation.
2nd, when excellent No. 1 Pelteobagrus fulvidraco of Huang, all-male Pelteobagrus fulvidraco and common yellow catfish growing are to 5-8cm, No. 1 Huang of Huang You is taken at random
The each 400-500 tails fry of forehead fish, all-male Pelteobagrus fulvidraco and common Pelteobagrus fulvidraco, selects neat specification, body surface without wound, physique healthy and strong
Yellow excellent No. 1 Pelteobagrus fulvidraco, all-male Pelteobagrus fulvidraco and each 180 tail of common Pelteobagrus fulvidraco, it is each to put in a suitable place to breed at random in 6 glass fibre culturing jars
(270L) in, per 30, cylinder.Cultured cause of disease Aeromonas hydrophila (Aeromonas hydrophila) is used into physiological saline
Concentration is diluted to for 1 × 106The bacterium solution of CFU/ml, in pectoral fin base portion bacterium solution 0.005ml/g fish body weight is injected, and challenge test respectively sets
One control group (physiological saline of injection same dose), 3 parallel, and experimental period is 1 week, and daily observed and recorded experiment fish is dead
Situation.Calculate the survival rate that each group tests fish, using survival rate come to " Huang You 1 " Pelteobagrus fulvidraco, all-male Pelteobagrus fulvidraco and common yellow
The anti-infection ability of forehead fish is compared evaluation.
Experimental result：Yellow excellent No. 1 Pelteobagrus fulvidraco, all-male Pelteobagrus fulvidraco, the growth performance of common Pelteobagrus fulvidraco and anti-infection ability are commented
Survey result as shown in table 2 below：
Yellow excellent No. 1, the all-male Pelteobagrus fulvidraco of table 2 and common yellow catfish growing performance and anti-infection ability determine
Note：Numerical value (mean+SD) is the mean value of 3 repetitions in table, and Superscript letters different after numerical value are represented
Significant difference (P<0.05).
Embodiment 3
1st, the screening of fast-growth hybrid strain：
1.1st, maternal screening：
In by the end of April, 2013 to by the end of November, collection comes from the lake of Yangtze river basin periphery (such as Liangzi Lake, Honghu, Dongting Lake
And Poyang Lake) wild Pelteobagrus fulvidraco, select body surface without wound, body weight more than 100g healthy and strong wild female Pelteobagrus fulvidraco as initial
Pelteobagrus fulvidraco is maternal；Select body surface without wound, body weight more than 250g healthy and strong wild males Pelteobagrus fulvidraco as initial Pelteobagrus fulvidraco male parent,
After the initial maternal and initial Pelteobagrus fulvidraco male parent of Pelteobagrus fulvidraco chooses, under the conditions of propagating artificially, the tame and docile foods of Jing can ingest hand fit
Initial Pelteobagrus fulvidraco parent culture is carried out after feed, 5-7 months in 2014, the gonad development degree of initial Pelteobagrus fulvidraco parent population is observed,
Select fully-developed Pelteobagrus fulvidraco parent to carry out breeding to hasten parturition, hatch and nurture of fish fingerlings, close in Pelteobagrus fulvidraco seed fast-growth
The key stage (the long 5-15cm of body), i.e., the 7-9 months of 2014, growth selection is fast, the individuality of high-output stress-resistance, selects and remain about 5%
Individuality is cultivated, until 3-4 months in 2015, most of individuality reaches sexal maturity, selects female as fast-growth hybridization
Pelteobagrus fulvidraco female parent (A systems), referred to as hybridizes maternal；
1.2nd, the screening of male parent：
Collect the Pelteobagrus vachelli from Yangtze river basin Yueyang section to Wuhan section, selective body to by the end of November in by the end of April, 2012
Table is maternal as initial Pelteobagrus vachelli in the healthy and strong wild female Pelteobagrus vachelli of more than 300g without wound, body weight；Select body surface
Without wound, body weight more than 500g healthy and strong wild males Pelteobagrus vachelli as initial Pelteobagrus vachelli male parent, initial Wa Shi is yellow
After the maternal and initial Pelteobagrus vachelli male parent of forehead fish chooses, under the conditions of propagating artificially, Jing tame and docile food can ingest hand fit feeding
Initial Pelteobagrus vachelli parent culture is carried out after material, 5-7 months in 2013, the gonad development of initial Pelteobagrus vachelli parent population is observed
Degree, selects the initial Pelteobagrus vachelli parent of fully-developed to carry out breeding and hastens parturition, hatches and nurture of fish fingerlings, in Wa Shi Huang foreheads
Fry kind fast-growth critical stage (the long 10-25cm of body), i.e., in 2013 7-9 months, growth selection is very fast, high-output stress-resistance
Individuality, about 5% individuality selected and remain is cultivated, to 3-4 months in 2015 or so, most of individuality reaches sexal maturity, selects male
Property as fast-growth hybridization Pelteobagrus fulvidraco male parent (B systems), abbreviation parent sire of hybrid pigs；
2nd, the reinforced cultivating of hybrid strain：
2.1st, maternal reinforced cultivating is hybridized：
The 3-4 months of 2015, select the well-developed hybridization female parent of sexual gland to render in the A of reinforced cultivating pond, put close in a suitable place to breed
Spend for 300 pounds/acre, wait hybridize it is maternal adapt to reinforced cultivating pond nurturing an environment after, start domestication and ingest special parent's mixed feed
(commercially available), day feeding volume is the 1-1.5% of the maternal gross weight of hybridization, is fed daily 2 times, and Feeding time is 6 points to 6 points of the morning
The feeding volume in the halfth, half to 7 points of 6 pm, the morning and afternoon accounts for respectively the 30% and 70% of day feeding volume, 1 before artificial induced spawning
Month, the fly maggot slurry that quality proportion is special parent's mixed feed quality 3% is added in special parent's mixed feed carries out 2 weeks
The maternal reinforced cultivating of hybridization, while being sampled inspection to hybridizing female parent every 1 week, checks the development feelings of the maternal sexual gland of hybridization
Condition；
2.2nd, the reinforced cultivating of parent sire of hybrid pigs：
The 3-4 months of 2015, select the well-developed parent sire of hybrid pigs of sexual gland to render in the B of reinforced cultivating pond, put close in a suitable place to breed
Spend for 280 pounds/acre, after parent sire of hybrid pigs adapts to reinforced cultivating pond nurturing an environment, start domestication and ingest special parent's mixed feed
(commercially available), day feeding volume is the 1-1.5% of parent sire of hybrid pigs gross weight, feeds daily 2 times, and Feeding time is 6 points to 6 points of the morning
The feeding volume in the halfth, half to 7 points of 6 pm, the morning and afternoon accounts for respectively the 30% and 70% of day feeding volume；
3rd, artificial induced spawning：
In May, 2015, after treating hybridization female parent and parent sire of hybrid pigs gonadal maturation, maternal and parent sire of hybrid pigs will be hybridized and divided
Do not throw in separate in two different spawning inducing pools and temporarily support, maternal and parent sire of hybrid pigs the input ratio of hybridization is calculated as 100 by tail：
1, two spawning inducing pool depth of waters are 0.5-0.6m, and two spawning inducing pool water temperatures are 25-30 DEG C, hybridize maternal breeding density and are
10kg/m2Water body, during hastening parturition, spawning inducing pool is in flowing water environment, and flowing water flow is 1.0m3/ h, treats that hybridization is injected for maternal second
After exogenous hormones, flowing water flow is increased, increase flowing water flow to 3.0m3/h；
Hybrid strain is temporarily supported and proceeds by artificial induced spawning after spawning inducing pool 3-5 hour, in hybridization female parent and parent sire of hybrid pigs abdomen
Chamber pectoral fin base portion injection of exogenous hormone, hybridization is maternal to inject exogenous hormones twice, twice time interval 12 hours, and hybridization is female
During this first time injection of exogenous hormone, exogenous hormones is by fish luteotropin releasing hormone d-ala analog and chorionic gonadotrophin
Hormone is constituted, and its each component consumption is as follows：Fish luteotropin releasing hormone d-ala analog 10ug/kg fish bodies, chorionic gonadotrophin
Hormone 150IU/kg fish bodies, when hybridizing maternal second injection of exogenous hormone, exogenous hormones is discharged by fish with luteotropin
Hormone analogs, human chorionic gonadtropin and maleic acid DOM composition, its each component consumption is as follows：Fish is released with luteotropin
Hormone analogs 20ug/kg fish bodies, human chorionic gonadtropin 2000IU/kg fish bodies, maleic acid DOM 4mg/kg fish bodies are put,
Parent sire of hybrid pigs injects an exogenous hormones, and the exogenous hormones of parent sire of hybrid pigs injection is similar to by fish with luteinizing hormone releasing hormone
Thing, human chorionic gonadtropin and maleic acid DOM composition, its each component consumption is as follows：Fish luteinizing hormone releasing hormone class
Like thing 5ug/kg fish bodies, human chorionic gonadtropin 600IU/kg fish bodies, maleic acid DOM 4mg/kg fish bodies.
4th, artificial hybridization fertilization
Hybridize maternal second injection of exogenous hormone after 12 hours, every half an hour sampling Detection maternal sexual gland row is hybridized
Ovate condition, when the individuality of 60-70% is attained by smoothly completing manually crowded ovum, you can start manually crowded ovum and receive ovum, receive every time
Intensive 100 tail raun fish-egg, you can the seminal fluid of the 1 tail hybridization father and mother for starting to prepare in advance be added in fish-egg carry out it is artificial
Insemination operation.
5th, artificial incubation：
To be hatched in embryonated egg incubator disposed within, hatching density is ten thousand embryonated eggs of 60-70/m3Water body, hatching
Flowing water environment is kept in case, makes embryonated egg in flowing water environment, flow velocity is 0.3m/s, adjusted current and ensure embryonated egg by just
Rush to water surface；
Embryonated egg starts 4-8 hours after demoulding, is in batches dragged for embryonated egg to stainless steel washbowl (diameter with the screen cloth of 80 mesh
In 40cm), this characteristic of dead ovum and mouldy embryonated egg is higher than using the density of the embryonated egg of normal development demoulding, is rejected
Fall dead ovum and mouldy embryonated egg, the embryonated egg of normal development is transferred in other off-the-shelf incubator afterwards continue into
Row hatching, can prevent normal fertilization ovum by mycotic infection, and conspicuousness improves the incubation rate of embryonated egg.
6th, the cultivation of seed：
After in incubator more than 95% embryonated egg demoulding 12 hours, yolk seedling is thrown in and carry out in cement pit temporarily
Culture, the long 10m of cement pit, width 1.5m, high 0.8m, depth of water 0.4-0.6m, breeding density is ten thousand tails of 5-10/m2Water body, yolk seedling
Start appearance after temporarily supporting 2-3 days and climb wall phenomenon on a small quantity, now yolk seedling is sold or thrown in carries out seed into special pond
Cultivation, 4 mu of special pond area, depth of water 0.6-1.0m, breeding density be ten thousand tails of 20-25/mu, hybrid seedlings Jing cultivate after,
Obtain yellow excellent No. 1 Pelteobagrus fulvidraco of big specification.
The growth performance and anti-infection ability evaluation and test experiment of yellow excellent No. 1 Pelteobagrus fulvidraco that experiment three, the present embodiment is bred
Experimental technique：
1st, when excellent No. 1 Pelteobagrus fulvidraco of Huang, all-male Pelteobagrus fulvidraco and common yellow catfish growing are to 5-8cm, No. 1 Huang of Huang You is taken at random
The each 400-500 tails of forehead fish, all-male Pelteobagrus fulvidraco and common Pelteobagrus fulvidraco, select neat specification, body surface without wound, physique healthy and strong it is yellow excellent
Each 90 tail of No. 1 Pelteobagrus fulvidraco, all-male Pelteobagrus fulvidraco and common Pelteobagrus fulvidraco, measures its average body length and body weight, as initial when putting in a suitable place to breed
Body length and body weight, then by yellow excellent No. 1 Pelteobagrus fulvidraco of 90 tails of select, all-male Pelteobagrus fulvidraco and common Pelteobagrus fulvidraco is each puts in a suitable place to breed at random 3
In individual glass fibre culturing jar (270L), per 30, cylinder, notes adjusting the weight of often nautilus between each cylinder without significant difference.Support
Sampled measurements are carried out to the body length and body weight of excellent No. 1 Pelteobagrus fulvidraco of Huang, all-male Pelteobagrus fulvidraco and common Pelteobagrus fulvidraco after growing 8 weeks, calculates yellow
Excellent No. 1 Pelteobagrus fulvidraco, all-male Pelteobagrus fulvidraco, the body length of common Pelteobagrus fulvidraco and the mean value of body weight, are increased using specific growth rate and body weight
Again rate to the growth performance of excellent No. 1 Pelteobagrus fulvidraco, all-male Pelteobagrus fulvidraco and common Pelteobagrus fulvidraco being compared evaluation.
2nd, when excellent No. 1 Pelteobagrus fulvidraco of Huang, all-male Pelteobagrus fulvidraco and common yellow catfish growing are to 5-8cm, No. 1 Huang of Huang You is taken at random
The each 400-500 tails fry of forehead fish, all-male Pelteobagrus fulvidraco and common Pelteobagrus fulvidraco, selects neat specification, body surface without wound, physique healthy and strong
Yellow excellent No. 1 Pelteobagrus fulvidraco, all-male Pelteobagrus fulvidraco and each 180 tail of common Pelteobagrus fulvidraco, it is each to put in a suitable place to breed at random in 6 glass fibre culturing jars
(270L) in, per 30, cylinder.Cultured cause of disease Aeromonas hydrophila (Aeromonas hydrophila) is used into physiological saline
Concentration is diluted to for 1 × 106The bacterium solution of CFU/ml, in pectoral fin base portion bacterium solution 0.005ml/g fish body weight is injected, and challenge test respectively sets
One control group (physiological saline of injection same dose), 3 parallel, and experimental period is 1 week, and daily observed and recorded experiment fish is dead
Situation.Calculate the survival rate that each group tests fish, using survival rate come to " Huang You 1 " Pelteobagrus fulvidraco, all-male Pelteobagrus fulvidraco and common yellow
The anti-infection ability of forehead fish is compared evaluation.
Experimental result：Yellow excellent No. 1 Pelteobagrus fulvidraco, all-male Pelteobagrus fulvidraco, the growth performance of common Pelteobagrus fulvidraco and anti-infection ability are commented
Survey result as shown in table 3 below：
Yellow excellent No. 1, the all-male Pelteobagrus fulvidraco of table 3 and common yellow catfish growing performance and anti-infection ability determine
Note：Numerical value (mean+SD) is the mean value of 3 repetitions in table, and Superscript letters different after numerical value are represented
Significant difference (P<0.05).
In sum, compare with all-male Pelteobagrus fulvidraco with common Pelteobagrus fulvidraco, the growth vigor of No. 1 Pelteobagrus fulvidraco of Huang You is extremely bright
It is aobvious, strong stress resistance.
Claims (5)
1. a kind of cross breeding method of yellow excellent No. 1 Pelteobagrus fulvidraco of improvement growth traits, comprises the steps：
1.1st, the screening of fast-growth hybrid strain：
1.1.1, maternal screening is hybridized：
Mid-April in the first year to by the end of November, select body surface without wound, body weight more than 100g wild female Pelteobagrus fulvidraco as initial Huang
Forehead fish is maternal；Select body surface without wound, body weight more than 250g wild males Pelteobagrus fulvidraco as initial Pelteobagrus fulvidraco male parent, it is initial yellow
After the maternal and initial Pelteobagrus fulvidraco male parent of forehead fish chooses, under the conditions of propagating artificially, Jing after tame and docile food can ingest artifical compound feed
Initial Pelteobagrus fulvidraco parent culture is carried out, in 5-7 months after year, the gonad development degree of initial Pelteobagrus fulvidraco parent population is observed, development is selected
Ripe initial Pelteobagrus fulvidraco parent carries out breeding and hastens parturition, hatches and nurture of fish fingerlings, in Pelteobagrus fulvidraco seed fast-growth key rank
Section, i.e., 7-9 months then, growth selection is fast, the individuality of high-output stress-resistance, and the individuality of the 4-6% that selects and remain is cultivated, Second Year
Or sexal maturity is reached within the 3rd year, and select the female individuals of fast-growth as hybridization Pelteobagrus fulvidraco female parent breeding line, referred to as hybridize female
This；
1.1.2, the screening of parent sire of hybrid pigs：
Mid-April in the first year to by the end of November, select body surface without wound, body weight more than 300g wild female Pelteobagrus vachelli as first
Beginning Pelteobagrus vachelli is maternal；Select body surface without wound, body weight more than 500g wild males Pelteobagrus vachelli as initial Wa Shi
Pelteobagrus fulvidraco male parent, after the maternal and initial Pelteobagrus vachelli male parent of initial Pelteobagrus vachelli chooses, under the conditions of propagating artificially, Jing
Tame and docile food can ingest the initial Pelteobagrus vachelli parent culture that carry out after artifical compound feed, in 5-7 months after year, observe initial Wa Shi yellow
The gonad development degree of forehead fish parent fish, select the initial Pelteobagrus vachelli parent of fully-developed carry out breeding hasten parturition, hatch and
Nurture of fish fingerlings, in Pelteobagrus vachelli seed fast-growth critical stage, i.e., 7-9 months then, growth selection is very fast, degeneration-resistant energy
The strong individuality of power, the individuality of the 1-2% that selects and remain is cultivated, and reaches within the 3rd year sexal maturity, and the male for selecting fast-growth is made
To hybridize Pelteobagrus fulvidraco male parent breeding line, abbreviation parent sire of hybrid pigs；
1.2nd, the reinforced cultivating of hybrid strain：
1.2.1, maternal reinforced cultivating is hybridized：
The well-developed hybridization female parent of sexual gland is selected to render in the A of reinforced cultivating pond, breeding density is 260-300 pound/acre, treats miscellaneous
Hand over it is maternal adapt to after the nurturing an environment of reinforced cultivating pond, start domestication and ingest special parent's mixed feed, day feeding volume is female for hybridization
The 1-1.5% of this gross weight, feeds daily 2 times, and Feeding time is half to 7 points of at 6 points in the morning to 6 thirty, 6 pm, the morning, under
The feeding volume at noon accounts for respectively 30-50%, 50-70% of day feeding volume, 1 month before artificial induced spawning, in special parent's mixed feed
Middle addition quality proportion is the reinforced cultivating of the hybridization female parent that the fly maggot slurry of special parent's mixed feed quality 3-5% is carried out 2 weeks,
Simultaneously inspection was sampled to hybridizing female parent every 1 week, checks the developmental state of the maternal sexual gland of hybridization；
1.2.2, the reinforced cultivating of parent sire of hybrid pigs：
The well-developed parent sire of hybrid pigs of sexual gland is selected to render in the B of reinforced cultivating pond, breeding density is 260-300 pound/acre, treats miscellaneous
Hand over male parent to adapt to after the nurturing an environment of reinforced cultivating pond, start domestication and ingest special parent's mixed feed, day feeding volume is hybridization father
The 1-1.5% of this gross weight, feeds daily 2 times, and Feeding time is half to 7 points of at 6 points in the morning to 6 thirty, 6 pm, the morning and
The feeding volume in afternoon accounts for respectively the 30-50% and 50-70% of day feeding volume；
1.3rd, artificial induced spawning：
After treating hybridization female parent and parent sire of hybrid pigs gonadal maturation, maternal and parent sire of hybrid pigs will be hybridized and thrown in respectively into two not
Separate in same spawning inducing pool and temporarily support, the input ratio of hybridization female parent and parent sire of hybrid pigs is calculated as 80-100 by tail：1, two spawning inducing pools
Water temperature is 25-30 DEG C, and it is 10-15kg/m to hybridize maternal breeding density2Water body, during hastening parturition, spawning inducing pool is in flowing water ring
Border, flowing water flow is 0.5-1.0m3/ h, after maternal second injection of exogenous hormone is hybridized, increases flowing water flow, increases stream
Discharge is to 2.0-3.0m3/h；
Hybrid strain is temporarily supported and proceeds by artificial induced spawning after spawning inducing pool 3-5 hour, in hybridization female parent and parent sire of hybrid pigs abdominal cavity chest
Fin base portion injection of exogenous hormone, hybridization is maternal to inject exogenous hormones twice, twice time interval 10-12 hour, and hybridization is female
During this first time injection of exogenous hormone, exogenous hormones is by fish luteotropin releasing hormone d-ala analog and chorionic gonadotrophin
Hormone is constituted, and its each component consumption is as follows：Fish luteotropin releasing hormone d-ala analog 10-16ug/kg fish bodies, chorion rush property
Glandular hormone 100-200IU/kg fish bodies；When hybridizing maternal second injection of exogenous hormone, exogenous hormones is by fish rush corpus luteum
Hormone-releasing hormone analog, human chorionic gonadtropin and maleic acid DOM composition, its each component consumption is as follows：Fish is yellow with promoting
Voxel releasing hormone analog 16-20ug/kg fish bodies, human chorionic gonadtropin 1200-2000IU/kg fish bodies, maleic acid ground
Europe ketone 4-8mg/kg fish bodies；Parent sire of hybrid pigs injects an exogenous hormones, and the exogenous hormones of parent sire of hybrid pigs injection is promoted by fish
Luteinizing hormone releasing hormone analog, human chorionic gonadtropin and maleic acid DOM composition, its each component consumption is as follows：Fish is used
Luteotropin releasing hormone d-ala analog 5-8ug/kg fish bodies, human chorionic gonadtropin 600-1000IU/kg fish bodies, maleic acid ground
Europe ketone 2.0-4.0mg/kg fish bodies；
1.4th, artificial hybridization fertilization
After hybridizing maternal second injection of exogenous hormone 8-12 hours, every half an hour sampling Detection maternal sexual gland ovulation is hybridized
Situation, when the individuality of 60-70% is attained by smoothly completing manually crowded ovum, starts manually crowded ovum and receives ovum, and 80- is collected every time
After 100 tail raun fish-eggs, the seminal fluid of the 1 tail parent sire of hybrid pigs for preparing in advance is added in the fish-egg of collection carries out artificial insemination
Operation；
1.5th, artificial incubation：
To be hatched in embryonated egg incubator disposed within, hatching density is ten thousand embryonated eggs of 60-70/m3Protect in water body, incubator
Flowing water environment is held, makes embryonated egg in flowing water environment, flow velocity is 0.2-0.3m/s；
Embryonated egg starts 4-8 hours after demoulding, is in batches dragged for embryonated egg into stainless steel washbowl, using normal development with screen cloth
The density of demoulding embryonated egg weeds out dead ovum and mouldy embryonated egg higher than dead ovum and this characteristic of mouldy embryonated egg, will
The embryonated egg of normal development is transferred in other off-the-shelf incubator and proceeds hatching；
1.6th, the cultivation of seed：
After in incubator more than 95% embryonated egg demoulding 12 hours, hybridization yolk seedling is thrown in cement pit and temporarily supported
Cultivate, breeding density is ten thousand tails of 5-10/m2Water body, yolk seedling starts appearance and climbs wall phenomenon on a small quantity after temporarily supporting 2-3 days, now by ovum
Huang Miao is sold or thrown in carries out seed rearing into off-the-shelf special pond, and breeding density is ten thousand tails of 20-25/mu, miscellaneous
Seed is handed over Jing after cultivating, you can obtain yellow excellent No. 1 Pelteobagrus fulvidraco of big specification.
2. the cross breeding method of yellow excellent No. 1 Pelteobagrus fulvidraco of improvement growth traits according to claim 1, its feature exists
In：The maternal and initial Pelteobagrus fulvidraco male parent of described initial Pelteobagrus fulvidraco is collection from the lake of Yangtze river basin periphery, described lake
For Liangzi Lake, Honghu, Dongting Lake or Poyang Lake, described wild Pelteobagrus vachelli is from Yangtze river basin Yueyang section to Wuhan section.
3. the cross breeding method of yellow excellent No. 1 Pelteobagrus fulvidraco of improvement growth traits according to claim 1, its feature exists
In：Described spawning inducing pool depth of water 0.5-0.6m.
4. the cross breeding method of yellow excellent No. 1 Pelteobagrus fulvidraco of improvement growth traits according to claim 1, its feature exists
In：During embryonated egg incubator hatching indoors, bolting silk net is filtered once every 1-2h cleanings, embryonated egg starts after demoulding, often
Bolting silk net is filtered every 0.5h cleanings once.
5. the cross breeding method of yellow excellent No. 1 Pelteobagrus fulvidraco of improvement growth traits according to claim 1, its feature exists
In：The long 5-10m of described cement pit, width 1.5-2.0m, high 0.8-1.2m, depth of water 0.4-0.6m, described special pond area
2-4 mus, depth of water 0.6-1.0m.
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