CN104255615A - Artificial propagation method of schizopygopsis malacanthus - Google Patents
Artificial propagation method of schizopygopsis malacanthus Download PDFInfo
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- CN104255615A CN104255615A CN201410537312.6A CN201410537312A CN104255615A CN 104255615 A CN104255615 A CN 104255615A CN 201410537312 A CN201410537312 A CN 201410537312A CN 104255615 A CN104255615 A CN 104255615A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/17—Hatching, e.g. incubators
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention provides an artificial propagation method of schizopygopsis malacanthus. The method comprises the following steps: 1, selecting pollution-free water; 2, collecting wild schizopygopsis malacanthus as backup parent fishes for domestication by using a net fishing mode; 3, selecting robust, disease-free, non-injury and nonteratogenic schizopygopsis malacanthus, of which the weight of each male fish is not less than 300 g and the weight of each female fish is not less than 400 g, as breeding parent fishes for breeding from the backup parent fishes of the wild schizopygopsis malacanthus after one-year domestication; 4, performing spawning induction preparation for the bred parent fishes bred for one year; 5, selecting mature parent fishes for artificial spawning induction; 6, performing artificial insemination on the parent fishes after artificial spawning induction; 7, performing artificial incubation by using a water flowing method. By using the method, the spawning induction rate of the schizopygopsis malacanthus reaches 80.00 percent, the fertilization rate reaches 63.48 percent and the hatching rate is 68.15 percent, large-scale artificial breeding of the schizopygopsis malacanthus can be realized, and the schizopygopsis malacanthus resource is effectively protected.
Description
Technical field
The present invention relates to that a kind of soft thorn is naked splits buttocks fish artificial fecundation method.
Background technology
Soft thorn is naked split buttocks fish (
schizopygopsis malacanthus)be under the jurisdiction of Cypriniformes, Cyprinidae, Schizothoracinae, Schizopygopsis, local by name: native fish, smallmouth fish etc., belong to cold water fishes, like the flow inlet place of Hui Shuichu and the plateau lake perched at Heavenly Stems and Earthly Branches stream, dwell life in the battalion end, winter survives the winter in cave, crack of stone, and its distribution height can reach height above sea level 3000-4000 rice.Take vegetal bait as food, often on rock, scrape food algae and submerged plant chip etc. with the cutin edge of lower jaw prosperity, also eat the blade of the aquatic microtubule fasolculus plant of part simultaneously.Soft thorn is naked to be split buttocks fish and is distributed in China's Jinsha jiang River middle and upper reaches and Yalongjiang River upstream water system, and be the medium-sized economic fish that in upstream branching flow and plateau lake, output is maximum, its delicious meat, being the kind having cultivation potentiality in cold water fish, is also Upper Yangtze River Endemic fish.
The research work splitting buttocks fish about soft thorn is naked, first carried out Classification And Nomenclature Revision (.2000. Fauna Sinica Osteichthyes Cypriniformes (last volume) such as happy pendant fine jade. Beijing: Science Press: 373-375.), Cao Wenxuan etc. have carried out Morbidity investigation and biological study
cao Wenxuan, 5 offer civilian .1962. western Sichuan Ganzi Aba area biology of fishes and fishing problem. Acta Hydrobiologica Sinica, 2:79-111.
wu Yunfei, Wu Cui precious .1992 Qinghai-Tibet Platean fish. Chengdu: Sichuan science tech publishing house: 1-599.
recklessly farsighted .2012. Jinsha jiang River upstream Status of Fisher Resources and protection. Postgraduate School, Chinese Academy of Sciences's master thesis, 1-91), Shen Danzhou, Hu Rui etc. carried out Age and growth research (
the naked Age Determination splitting buttocks fish of the soft thorn of the refined .2007. of Shen Danzhou, He Chunlin, Song Zhao. Sichuan animal, 26(1): 124-125.
hu Rui, Wang Jianwei, Tan Deqing, Miao Zhiguo, but the naked research splitting buttocks fish Age and growth of the victory soft thorn in upstream, state .2012. Jinsha jiang River. Sichuan animal, 31 (5): 708-712,719.).Soft thorn is naked, and to split buttocks fish be the fish producing demersal egg in miniflow water, and along with Jinsha jiang River middle and upper reaches Construction of Step Hydropower Station, the atrophy of flowing water habitat, produces the naked existence splitting buttocks fish of soft thorn and have a strong impact on, but have no the research report splitting the artificial propagation of buttocks fish about soft thorn is naked at present.
Summary of the invention
The object of the invention is for above-mentioned Problems existing, provide that a set of soft thorn is naked splits buttocks fish artificial fecundation method, protect that soft thorn is naked splits buttocks fish resource, and realize that soft thorn is naked splits buttocks fish scale artificial culture.The present invention is achieved in that
1, water quality requirement: cultivate water is pure and fresh, pollution-free, and dissolved oxygen is not less than 5mg/L, pH value 6.5-7.5, water temperature 0-20 DEG C;
2, parent population collection and raise and train: collect in the net mode of catching that the wild soft thorn of more than body weight 200g is naked splits buttocks fish parent population in support, rendered to by reserve parent fish to raise and train in pond and carry out domesticating and cultivating, raising and train pool area is 100-1000m
2, at the bottom of pond, ratio reduces to 2-3%, and pond height is 1.5-2.0m, and the depth of water is 1-1.5m, and discharge is 0.1-0.2m
3/ s, every square metre of breeding density is no more than 3kg, and raising and train feed is carp commercial feed, protein content 35-45%, and every day, feeding volume was the 1-2% of fish body weight, and every day respectively throws something and feeds once sooner or later;
3, the screening of parent population and cultivation is bred: select that milter body weight is not less than 300g/ tail, raun body weight is not less than 400g/ tail physique is healthy and strong, anosis splits buttocks fish as breeding parent population without wound without the soft thorn of deformity is naked from naked the splitting buttocks fish reserve parent fish of the wild soft thorn of raising and train through 1 year, parent fish rearing pool area is 20-50m
2, at the bottom of pond, ratio reduces to 1-2%, and pond height is 1.5-2.0m, and the depth of water is 1-1.5m, and discharge is 0.1-0.2m
3/ s, every square metre of breeding density is no more than 3kg, and raising and train feed is carp commercial feed, protein content 35-45%, and every day, feeding volume was the 1-4% of fish body weight, and every day respectively throws something and feeds once sooner or later;
4, to hasten parturition preparation: the breeding parent population cultivated through 1 year, in the 3-5 month, the sexual maturity situation of inspection 1 parent population is drawn in the net weekly in parent fish rearing pond, inspection method is: by the raun fish body of maturation upwards, slightly dew face, ovary profile is obviously seen in both sides, presses belly with have gentle hands, and soft high resilience is sexual maturity; Milter " pearl star " is obvious, and the gap between anal fin 4-6 root branch fin ray is strengthened, and be significantly hook-shaped in end specialization, hand touches body surface very strong harsh feeling, and upwards, light pressure belly both sides, have milky dense thick seminal fluid to flow out for sexual maturity to fish body belly; Before hastening parturition, strengthen parent fish rearing Chi Shui turnover amount, Chi Shui exchange per hour once, promotes parent population sexual maturity;
5, artificial induced spawning: select sexually matured raun, at its pectoral fin base portion injection fish ocyodinic, ocyodinic divides 1-4 injection, if 2-4 injection, with the 1st injected dose for benchmark, 2nd time injected dose is higher than the 1st time 1 times, 3rd time injected dose is higher than the 2nd time 1 times, the 4th injected dose is higher than the 3rd time 1 times, 24 hours double injection time intervals, the active situation of raun is observed after per injection, if there is phenomenon of laying eggs, just carry out artificial insemination, no longer carry out injection below, milter is also injected at its pectoral fin base portion, ocyodinic consumption is the half of raun, the no longer injection ocyodinic that milter sexual maturity is good, fish ocyodinic can select Central-South No. 3 of fish PG, LRH, DOM, HCG, fish hormone, can with two kinds or three kinds of ocyodinic compatibilities according to the developmental state of parent population, various ocyodinic injected dose is respectively PG:1-20mg/kg, LRH:1-20ug/kg, DOM:1-20mg/kg, HCG:500-1000 unit/kg, fish hormone Central-South No. 3: 1-10ml/kg, as 1 injection, injection compatibility is LRH10ug/kg, HCG1000 unit/kg, DOM5mg/kg, as point 3 injections, first time injection fish LRH1ug/kg, DOM1mg/kg, second time injection fish LRH2ug/kg, DOM2mg/kg, third time injection fish LRH4ug/kg, DOM4mg/kg, HCG1000 unit/kg,
6, artificial insemination, puts into spawning pond by the parent population of injecting ocyodinic, spawning pond area 1-3m
2, pond height 0.7-1.0m, about depth of water 0.5m, the same pond of male and female, ceaselessly put stimulation by running water, observe parent population active situation at any time, when raun oestrus reach a climax time, if light pressure raun belly, fish-egg flows out, raun is mentioned, wipe body surface moisture, squeeze in ovum engagement, exist side by side and to mention by milter, wipe body surface moisture, rapidly seminal fluid is clamp-oned in basin, fish-egg 1-2 minute is stirred with feather, smart ovum is fully mixed, add a small amount of clear water again, stir slightly, leave standstill 1-2 minute, then ovum is washed 3-6 time with clear water, wash away the water of seminal fluid, add water and be placed on lucifuge place, sunlight and light direct projection should be avoided in whole operating process, prevent ultraviolet to the injury of sperm with insemination ovum, after artificial insemination, rehabilitation cultivation is carried out to parent population in postpartum,
7, artificial incubation: adopt Lotic hatching, incubator box is put into incubation tank, puts into insemination ovum after adding water, it is 3-6 grain Luan/㎝ that each incubator box puts into density
2hatch, incubator box depth of water 10-15cm, hatching water quality requirement is with step 1, and at water temperature 12-18 DEG C, through the hatching of 149-183 hour, fertilized egg just starts rupture of membranes and emerges.
Using method of the present invention to carry out, soft thorn is naked splits the artificial propagation of buttocks fish, and soft thorn is naked splits that buttocks fish spawning rate reaches 80.00%, fertilization rate 63.48%, incubation rate 68.15%.Can realize that soft thorn is naked splits buttocks fish scale artificial culture, the soft thorn of available protecting is naked splits buttocks fish resource.
Embodiment
1, water quality requirement: cultivate water is pure and fresh, pollution-free, and dissolved oxygen is not less than 5mg/L, pH value 6.5-7.5, water temperature 0-20 DEG C, springwater, underground water, streams water, reservoir bottom water can be selected in water source;
2, parent population collection and raise and train: collect in the net mode of catching that the wild soft thorn of more than body weight 200g is naked splits buttocks fish parent population in support, rendered to by reserve parent fish to raise and train in pond and carry out domesticating and cultivating, raising and train pool area is 100-1000m
2, at the bottom of pond, ratio reduces to 2-3%, is convenient to release water, and pond height is 1.5-2.0m, and the depth of water is 1-1.5m, and discharge is 0.1-0.2m
3/ s, every square metre of breeding density is no more than 3kg, and raising and train pond can be cement pit, also can be the fishpond of natural material, build up and prevent escaping facility, be equipped with culvert system.Raising and train feed is carp commercial feed, and protein content 35-45% can select the carp commercial feed that Tong Wei feed companies produces; Every day, feeding volume was the 1-2% of fish body weight, and every day respectively throws something and feeds once sooner or later;
3, the screening of parent population and cultivation is bred: select that milter body weight is not less than 300g/ tail, raun body weight is not less than 400g/ tail physique is healthy and strong, anosis splits buttocks fish as breeding parent population without wound without the soft thorn of deformity is naked from naked the splitting buttocks fish reserve parent fish of the wild soft thorn of raising and train through 1 year, parent fish rearing pool area is 20-50m
2, at the bottom of pond, ratio reduces to 1-2%, is convenient to release water, and pond height is 1.5-2.0m, and the depth of water is 1-1.5m, and be equipped with and prevent escaping facility and culvert system, discharge is 0.1-0.2 m
3/ s, every square metre of breeding density is no more than 3kg, and raising and train feed is carp commercial feed, and protein content 35-45% can select the carp commercial feed that Tong Wei feed companies produces; Every day, feeding volume was the 1-4% of fish body weight, and every day respectively throws something and feeds once sooner or later;
4, to hasten parturition preparation: the breeding parent population cultivated through 1 year, in the 3-5 month, the sexual maturity situation of inspection 1 parent population is drawn in the net weekly in parent fish rearing pond, inspection method is: by the raun fish body of maturation upwards, slightly dew face, ovary profile is obviously seen in both sides, presses belly with have gentle hands, and soft high resilience is sexual maturity; Milter " pearl star " is obvious, and the gap between anal fin 4-6 root branch fin ray is strengthened, and is significantly hook-shaped in end specialization, hand touches body surface very strong harsh feeling, and fish body belly upwards, gently presses belly both sides, have milky dense thick seminal fluid to flow out for sexual maturity, it is best for meeting the water person that do not scatter rapidly; Before hastening parturition, strengthen parent fish rearing Chi Shui turnover amount, Chi Shui exchange per hour once, promotes parent population sexual maturity;
5, artificial induced spawning: select sexually matured raun, at its pectoral fin base portion injection fish ocyodinic, ocyodinic divides 1-4 injection, if 2-4 injection, with the 1st injected dose for benchmark, 2nd time injected dose is higher than the 1st time 1 times, 3rd time injected dose is higher than the 2nd time 1 times, the 4th injected dose is higher than the 3rd time 1 times, 24 hours double injection time intervals, the active situation of raun is observed after per injection, if there is phenomenon of laying eggs, just carry out artificial insemination, no longer carry out injection below, milter is also injected at its pectoral fin base portion, ocyodinic consumption is the half of raun, the no longer injection ocyodinic that milter sexual maturity is good, fish ocyodinic can select fish PG(carp brain hypophysis-Peng Liuyang road, wuchang, wuhan district aquatic products fishing estovers business department to provide), LRH(injection luteotropin analog releasing hormone-Ningbo Sansheng Pharmaceutical Co., Ltd. produces), DOM(maleic acid DOM-Ningbo Sansheng Pharmaceutical Co., Ltd. produces), HCG(pregnyl ampoule-Ningbo Sansheng Pharmaceutical Co., Ltd. produces), fish is with Central-South No. 3 of hormone (Guangzhou Central-South Bioisystech Co., Ltd produce), can with two kinds or three kinds of ocyodinic compatibilities according to the developmental state of parent population, various ocyodinic injected dose is respectively PG:1-20mg/kg, LRH:1-20ug/kg, DOM:1-20mg/kg, HCG:500-1000 unit/kg, fish hormone Central-South No. 3: 1-10ml/kg,
6, artificial insemination, puts into spawning pond by the parent population of injecting ocyodinic, spawning pond area 1-3m
2, pond height 0.7-1.0m, about depth of water 0.5m, the same pond of male and female, ceaselessly put stimulation by running water, observe parent population active situation at any time, when raun oestrus reach a climax time, if light pressure raun belly, fish-egg flows out, raun is mentioned, wipe body surface moisture, squeeze in ovum engagement, exist side by side and to mention by milter, wipe body surface moisture, rapidly seminal fluid is clamp-oned in basin, fish-egg 1-2 minute is stirred with feather, smart ovum is fully mixed, add a small amount of clear water again, stir slightly, leave standstill 1-2 minute, then ovum is washed 3-6 time with clear water, wash away the water of seminal fluid, add water and be placed on lucifuge place, sunlight and light direct projection should be avoided in whole operating process, prevent ultraviolet to the injury of sperm with insemination ovum, after artificial insemination, to parent population injection compound gentamicine sulphate in postpartum (4000 units/kg) or penicillin (10000 units/kg), carry out rehabilitation cultivation,
7, artificial incubation: adopt Lotic hatching, incubator box is put into incubation tank, puts into insemination ovum after adding water, it is 3-6 grain Luan/㎝ that each incubator box puts into density
2hatch, incubator box depth of water 10-15cm, hatching water quality requirement is with step 1, and at water temperature 12-18 DEG C, through the hatching of 149-183 hour, fertilized egg just starts rupture of membranes and emerges.Incubation tank and incubator box are that glass-reinforced plastic material is made, and specification is: hatching flute length 2-3m, wide 0.4m, dark 0.17m; The long 0.4m of incubator box, wide 0.4m, dark 0.15m, at incubator box liner 40 object screen cloth, water freely can exchange at in-out-snap.
Claims (2)
1. soft thorn is naked splits a buttocks fish artificial fecundation method, it is characterized in that:
(1) water quality requirement: cultivate water is pure and fresh, pollution-free, and dissolved oxygen is not less than 5mg/L, pH value 6.5-7.5, water temperature 0-20 DEG C;
(2) parent population collection and raise and train: collect in the net mode of catching that the wild soft thorn of more than body weight 200g is naked splits buttocks fish parent population in support, rendered to by reserve parent fish to raise and train in pond and carry out domesticating and cultivating, raising and train pool area is 100-1000m
2, at the bottom of pond, ratio reduces to 2-3%, and pond height is 1.5-2.0m, and the depth of water is 1-1.5m, and discharge is 0.1-0.2m
3/ s, every square metre of breeding density is no more than 3kg, and raising and train feed is carp commercial feed, protein content 35-45%, and every day, feeding volume was the 1-2% of fish body weight, and every day respectively throws something and feeds once sooner or later;
(3) screening of parent population and cultivation is bred: select that milter body weight is not less than 300g/ tail, raun body weight is not less than 400g/ tail physique is healthy and strong, anosis splits buttocks fish as breeding parent population without wound without the soft thorn of deformity is naked from naked the splitting buttocks fish reserve parent fish of the wild soft thorn of raising and train through 1 year, parent fish rearing pool area is 20-50m
2, at the bottom of pond, ratio reduces to 1-2%, and pond height is 1.5-2.0m, and the depth of water is 1-1.5m, and discharge is 0.1-0.2m
3/ s, every square metre of breeding density is no more than 3kg, and raising and train feed is carp commercial feed, protein content 35-45%, and every day, feeding volume was the 1-4% of fish body weight, and every day respectively throws something and feeds once sooner or later;
(4) to hasten parturition preparation: the breeding parent population cultivated through 1 year, in the 3-5 month, the sexual maturity situation of inspection 1 parent population is drawn in the net weekly in parent fish rearing pond, inspection method is: by the raun fish body of maturation upwards, slightly dew face, ovary profile is obviously seen in both sides, presses belly with have gentle hands, and soft high resilience is sexual maturity; Milter " pearl star " is obvious, and the gap between anal fin 4-6 root branch fin ray is strengthened, and be significantly hook-shaped in end specialization, hand touches body surface very strong harsh feeling, and upwards, light pressure belly both sides, have milky dense thick seminal fluid to flow out for sexual maturity to fish body belly; Before hastening parturition, strengthen parent fish rearing Chi Shui turnover amount, Chi Shui exchange per hour once, promotes parent population sexual maturity;
(5) artificial induced spawning: select sexually matured raun, at its pectoral fin base portion injection fish ocyodinic, ocyodinic divides 1-4 injection, if 2-4 injection, with the 1st injected dose for benchmark, 2nd time injected dose is higher than the 1st time 1 times, 3rd time injected dose is higher than the 2nd time 1 times, the 4th injected dose is higher than the 3rd time 1 times, 24 hours double injection time intervals, the active situation of raun is observed after per injection, if there is phenomenon of laying eggs, just carry out artificial insemination, no longer carry out injection below, milter is also injected at its pectoral fin base portion, ocyodinic consumption is the half of raun, the no longer injection ocyodinic that milter sexual maturity is good, fish ocyodinic can select Central-South No. 3 of fish PG, LRH, DOM, HCG, fish hormone, can with two kinds or three kinds of ocyodinic compatibilities according to the developmental state of parent population, various ocyodinic injected dose is respectively PG:1-20mg/kg, LRH:1-20ug/kg, DOM:1-20mg/kg, HCG:500-1000 unit/kg, fish hormone Central-South No. 3: 1-10ml/kg,
(6) artificial insemination, puts into spawning pond by the parent population of injecting ocyodinic, spawning pond area 1-3m
2, pond height 0.7-1.0m, about depth of water 0.5m, the same pond of male and female, ceaselessly put stimulation by running water, observe parent population active situation at any time, when raun oestrus reach a climax time, if light pressure raun belly, fish-egg flows out, raun is mentioned, wipe body surface moisture, squeeze in ovum engagement, exist side by side and to mention by milter, wipe body surface moisture, rapidly seminal fluid is clamp-oned in basin, fish-egg 1-2 minute is stirred with feather, smart ovum is fully mixed, add a small amount of clear water again, stir slightly, leave standstill 1-2 minute, then ovum is washed 3-6 time with clear water, wash away the water of seminal fluid, add water and be placed on lucifuge place, sunlight and light direct projection should be avoided in whole operating process, prevent ultraviolet to the injury of sperm with insemination ovum, after artificial insemination, rehabilitation cultivation is carried out to parent population in postpartum,
(7) artificial incubation: adopt Lotic hatching, incubator box is put into incubation tank, puts into insemination ovum after adding water, it is 3-6 grain Luan/㎝ that each incubator box puts into density
2hatch, incubator box depth of water 10-15cm, hatching water quality requirement is with step 1, and at water temperature 12-18 DEG C, through the hatching of 149-183 hour, fertilized egg just starts rupture of membranes and emerges.
2. soft thorn according to claim 1 is naked splits buttocks fish artificial fecundation method, and it is characterized in that incubation tank and incubator box are that glass-reinforced plastic material is made, specification is: hatching flute length 2-3m, wide 0.4m, dark 0.17m; The long 0.4m of incubator box, wide 0.4m, dark 0.15m, at incubator box liner 40 object screen cloth.
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|---|---|---|---|---|
| CN104585095A (en) * | 2015-01-20 | 2015-05-06 | 青岛龙宫海洋牧场生态修复工程有限公司 | Culturing method of aurelia scyphistoma |
| CN106538429A (en) * | 2016-11-15 | 2017-03-29 | 丽江林昊生态养殖有限公司 | A kind of naked cultural method for splitting buttocks fish of soft thorn |
| CN106818550A (en) * | 2016-12-28 | 2017-06-13 | 贵州锦润水产品有限责任公司 | A kind of naked cultural method for splitting buttocks fish of soft thorn |
| CN106879510A (en) * | 2017-04-12 | 2017-06-23 | 西藏自治区农牧科学院水产科学研究所 | The induced spawning method of the naked row buttocks fish in Lhasa |
| CN116287289A (en) * | 2023-01-09 | 2023-06-23 | 中国长江三峡集团有限公司中华鲟研究所 | Identification method of white turtle germplasm and application of white turtle germplasm identification method in polyculture method of white turtle, soft-thorn nojirimi fish and red eye trout |
| CN116287289B (en) * | 2023-01-09 | 2023-10-13 | 中国长江三峡集团有限公司中华鲟研究所 | Identification method of white turtle germplasm and application of white turtle germplasm identification method in polyculture method of white turtle, soft-thorn nojirimi fish and red eye trout |
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