CN104737977A - Artificial spawning inducing and inseminating method for giant salamander - Google Patents
Artificial spawning inducing and inseminating method for giant salamander Download PDFInfo
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- CN104737977A CN104737977A CN201510103806.8A CN201510103806A CN104737977A CN 104737977 A CN104737977 A CN 104737977A CN 201510103806 A CN201510103806 A CN 201510103806A CN 104737977 A CN104737977 A CN 104737977A
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Abstract
The invention discloses an artificial spawning inducing and inseminating method for giant salamander. The method includes the steps that mature female giant salamander parents and mature male giant salamander parents are selected, an estrus promoting hormone is injected into the mature giant salamander parents, then the giant salamander parents are put into an artificial spawning inducing pond, the water temperature is controlled to be 15 DEG C-23 DEG C, and the mature giant salamander parents spawn or discharge seminal fluid within 4-9 days; when starting to produce egg straps, the female giant salamander parents are lifted from water and put into a cloth stretcher, the tails are lifted upwards, the egg straps naturally fall into a basin without water, the abdomen seminal fluid of the male giant salamander parents is extruded and covers the egg straps, a small amount of water is added, the basin is shaken slightly to make sperms and eggs be combined fully, clear water is added after 5-10 min, and water is changed once at intervals of 30 min, after the water is changed twice, the mixture can be divided into other basins to enter an incubation stage, and artificial spawning inducing and inseminating for the giant salamander are completed. Effective control is achieved according to the living environment of the giant salamander, natural risks and natural enemy harms are avoided, the artificial spawning inducing and inseminating rate can be 70-75%, and the reproductive rate and economic benefits of the giant salamander are greatly increased.
Description
Technical field
The invention belongs to aquaculture field, be specifically related to a kind of giant salamander artificial induced spawning and insemination method.
Background technology
Giant salamander belongs to Amphibia Caudata Cryptobranchidae, and the local name common name giant salamander is a kind of rare precious animal.This kind of animal only distribution of faults in east Asia and eastern united states.The giant salamander of China is the one that Modern distribution is the widest, resource is the abundantest.Main product is in the Changjiang river, and the small stream korneforos, mountain area in the Yellow River and Zhujiang River middle and upper reaches tributary, throughout 17 provinces (district), economizes as many with Guizhou, Sichuan, Hubei, Hunan, Shaanxi etc. especially.Because giant salamander living condition is high, reproduction rate is low, causes giant salamander population quantity sharply to reduce.
Summary of the invention
The object of the invention is for above problem, a kind of giant salamander artificial induced spawning and insemination method is provided, effectively can improves the reproduction rate of giant salamander.
For solving the problems of the technologies described above, the technical solution adopted in the present invention is: a kind of giant salamander artificial induced spawning and insemination method, and concrete steps are:
1) select ripe giant salamander parent, female giant salamander must to be expanded and soft by belly, touches belly, has full lax sense, the bossed milky point of male giant salamander cloacal aperture periphery, and have tangerine flap muscle projection around cloacal aperture, inner rim redness with have gentle hands;
2) inject to the giant salamander parent of maturation hormone of promoting the sexual maturity, then put into artificial induced spawning pond, control water temperature 15-23 DEG C, ripe giant salamander parent was laid eggs or ejaculation in 4-9 days;
3) when female giant salamander is in artificial induced spawning pond during output chalaza, it is mentioned from water and puts into cloth stretcher, and its eyes cloth is muffled, then its afterbody is upwards mentioned by a people, another people holds anhydrous basin, and with have gentle hands holder chalaza, allow chalaza naturally hold in the palm in engagement, then the belly of the male giant salamander of light pressure, crowded extracting extract is placed on chalaza, add 3-4ml water, shake gently, smart ovum is fully combined, after 5-10min, add clear water, every 30min changes water once, basin can be divided to enter incubation period after changing twice;
By above-mentioned steps, complete the artificial induced spawning and insemination of giant salamander.
Injection promote the sexual maturity hormone time, adopt human chorionic gonadtropin and luteinizing hormone-releasing hormone analog.
In step 1), before selecting ripe female giant salamander parent, held up by female giant salamander health, abdomen after light pressure, selects after extruding urine again.Because as cross belly expand time, due to giant salamander skin smooth, muscle is plump, and bladder storage urine volume is many, or when including indigested food in stomach, first urine is not discharged or light pressure belly is not easy to be confused by false appearance and causes erroneous judgement.
Step 2) in, when male giant salamander can squeeze extracting extract, microexamination is carried out to seminal fluid, to select the mature sperm with good fertilizing ability; Ripe sperm has that quantity is many, in single, when dripping, sperm can swing or feature travelling forward end to end.For the male giant salamander can extruding the outstanding sperm of quality, should lay special stress on protecting, in order to avoid cause the waste of seminal fluid.
In step 3), during artificial holder ovum, if chalaza can not natural labor, firmly can not entrust by force, must action treat without proper respect, gently towing force downwards, the output in auxiliary chalaza.If held out by force, or overexert, be easy to cause ossphere to be out of shape, output is given up ovum.
Need in step 3) to carry out in indoor, light is effectively covered, provide good living environment for giant salamander.
Beneficial effect of the present invention:
Giant salamander breeds fertilization rate only between 50-57% under natural surroundings, and adopt artificial induced spawning technology provided by the invention, living environment according to giant salamander control effectively, avoid natural risk and natural enemy harm, environmental condition and safety reliable, artificial induced spawning fertilization rate can reach 70-75%, substantially increases reproduction rate and the economic benefit of giant salamander.
Embodiment
Below in conjunction with concrete enforcement, illustrate the present invention further.These embodiments are interpreted as only for illustration of the present invention instead of for limiting the scope of the invention.After the content of having read the present invention's record, those skilled in the art can make various changes or modifications the present invention, and these equivalence changes and modification fall into claims of the present invention limited range equally.
Embodiment 1:
A kind of giant salamander artificial induced spawning and insemination method, concrete steps are:
1) select ripe giant salamander parent, female giant salamander must to be expanded and soft by belly, touches belly, has full lax sense, the bossed milky point of male giant salamander cloacal aperture periphery, and have tangerine flap muscle projection around cloacal aperture, inner rim redness with have gentle hands;
2) to promote the sexual maturity hormone to the injection of the giant salamander parent of maturation, then put into artificial induced spawning pond, control water temperature 15-23 DEG C, ripe giant salamander parent was laid eggs ejaculation in 4-9 days;
3) when female giant salamander is in artificial induced spawning pond during output chalaza, it is mentioned from water and puts into cloth stretcher, and its eyes cloth is muffled, then its afterbody is upwards mentioned by a people, another people holds anhydrous basin, and with have gentle hands holder chalaza, allow chalaza naturally hold in the palm in engagement, then the belly of the male giant salamander of light pressure, crowded extracting extract is placed on chalaza, add 3-4ml water, shake gently, smart ovum is fully combined, after 5-10min, add clear water, every 30min changes water once, basin can be divided to enter incubation period after changing twice;
By above-mentioned steps, complete the artificial induced spawning and insemination of giant salamander.After hatching, calculate fertilization rate is 72%.
Embodiment 2:
A kind of giant salamander artificial induced spawning and insemination method, concrete steps are:
1) ripe giant salamander parent is selected, before selecting ripe female giant salamander parent, held up by female giant salamander health, abdomen after light pressure, selects after extruding urine again, female giant salamander must to be expanded and soft by belly, touch belly with have gentle hands, have full lax sense, the bossed milky point of male giant salamander cloacal aperture periphery, and around cloacal aperture, having tangerine flap muscle protruding, inner rim is red and swollen;
2) to promote the sexual maturity hormone to the injection of the giant salamander parent of maturation, then put into artificial induced spawning pond, control water temperature 15-23 DEG C, ripe giant salamander parent was laid eggs ejaculation in 4-9 days;
3) when female giant salamander is in artificial induced spawning pond during output chalaza, it is mentioned from water and puts into cloth stretcher, and its eyes cloth is muffled, then its afterbody is upwards mentioned by a people, another people holds anhydrous basin, and with have gentle hands holder chalaza, allow chalaza naturally hold in the palm in engagement, then the belly of the male giant salamander of light pressure, crowded extracting extract is placed on chalaza, add 3-4ml water, shake gently, smart ovum is fully combined, after 5-10min, add clear water, every 30min changes water once, basin can be divided to enter incubation period after changing twice;
By above-mentioned steps, complete the artificial induced spawning and insemination of giant salamander, after hatching, calculate fertilization rate is 70%.
Embodiment 3:
A kind of giant salamander artificial induced spawning and insemination method, concrete steps are:
1) select ripe giant salamander parent, female giant salamander must to be expanded and soft by belly, touches belly, has full lax sense, the bossed milky point of male giant salamander cloacal aperture periphery, and have tangerine flap muscle projection around cloacal aperture, inner rim redness with have gentle hands;
2) to promote the sexual maturity hormone to the injection of the giant salamander parent of maturation, then put into artificial induced spawning pond, control water temperature 15-23 DEG C, ripe giant salamander parent was laid eggs ejaculation in 4-9 days; When male giant salamander can squeeze extracting extract, microexamination is carried out to seminal fluid, to select the mature sperm with good fertilizing ability; Ripe sperm has that quantity is many, in single, when dripping, sperm can swing or feature travelling forward end to end, and this type of male giant salamander should be laid special stress on protecting, and the sperm of output also can freezen protective simultaneously, inseminates after thawing when female giant salamander lays eggs;
3) when female giant salamander is in artificial induced spawning pond during output chalaza, it is mentioned from water and puts into cloth stretcher, and its eyes cloth is muffled, then its afterbody is upwards mentioned by a people, another people holds anhydrous basin, and with have gentle hands holder chalaza, allow chalaza naturally hold in the palm in engagement, then the belly of the male giant salamander of light pressure, crowded extracting extract is placed on chalaza, add 3-4ml water, shake gently, smart ovum is fully combined, after 5-10min, add clear water, every 30min changes water once, basin can be divided to enter incubation period after changing twice;
By above-mentioned steps, complete the artificial induced spawning and insemination of giant salamander, after hatching, calculate fertilization rate is 75%.
In addition, during artificial holder ovum operation, if chalaza can not natural labor, firmly can not entrust by force, must action treat without proper respect, gently towing force downwards, the output in auxiliary chalaza, and preferably carry out in indoor in step 3).
During the artificial incubation of fertilized egg, after the giant salamander feritilization of ovum, first time change is direction of halting, namely animal pole upwards, the downward protoplasm of vegetative pole is concentrated to animal pole, form blastodisc, then there is obvious first point of dehiscence furrow, form two cells, enter four cells again, second point of dehiscence furrow is vertical with first division ditch, egg cell sizableness, marshalling, this is the early stage feature of giant salamander ovum, if division is normal, namely be fertilized egg at last, otherwise it is as abnormal in 2-4 cell division, then often can not grow, this is unfertilized performance, to multicellular stage, cell arrangement is irregular, blastula stage, cell was superfine, not easily observe, to neurula stage, namely there is neural plate or neural groove phase, such giant salamander ovum embryo is fertilized egg certainly.The correct estimation of fertilized egg, makes artificial incubation accomplish to know what's what, and separates unfertilized ovum in time, and this is very important to raising incubation rate.
Claims (5)
1. a giant salamander artificial induced spawning and insemination method, is characterized in that, concrete steps are:
1) select ripe giant salamander parent, female giant salamander must to be expanded and soft by belly, touches belly, has full lax sense, the bossed milky point of male giant salamander cloacal aperture periphery, and have tangerine flap muscle projection around cloacal aperture, inner rim redness with have gentle hands;
2) to promote the sexual maturity hormone to the injection of the giant salamander parent of maturation, then put into artificial induced spawning pond, control water temperature 15-23 DEG C, ripe giant salamander parent was laid eggs ejaculation in 4-9 days;
3) when female giant salamander is in artificial induced spawning pond during output chalaza, it is mentioned from water and puts into cloth stretcher, and its eyes cloth is muffled, then its afterbody is upwards mentioned by a people, another people holds anhydrous basin, and with have gentle hands holder chalaza, allow chalaza naturally hold in the palm in engagement, then the belly of the male giant salamander of light pressure, crowded extracting extract is placed on chalaza, add 3-4ml water, shake gently, smart ovum is fully combined, after 5-10min, add clear water, every 30min changes water once, basin can be divided to enter incubation period after changing twice;
By above-mentioned steps, complete the artificial induced spawning and insemination of giant salamander.
2. giant salamander artificial induced spawning and insemination method according to claim 1, is characterized in that: in step 1), before selecting ripe female giant salamander parent, is held up by female giant salamander health, and abdomen after light pressure, selects after extruding urine again.
3. giant salamander artificial induced spawning and insemination method according to claim 1, is characterized in that: step 2) in, when male giant salamander can squeeze extracting extract, microexamination is carried out to seminal fluid, to select the mature sperm with good fertilizing ability; Ripe sperm has that quantity is many, in single, when dripping, sperm can swing or feature travelling forward end to end.
4. giant salamander artificial induced spawning and insemination method according to claim 1, is characterized in that: in step 3), during artificial holder ovum, if chalaza can not natural labor, firmly can not entrust by force, must action treat without proper respect, gently towing force downwards, the output in auxiliary chalaza.
5. the giant salamander artificial induced spawning and insemination method according to claim 1-4 any one, is characterized in that: need in step 3) to carry out in indoor.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107646798A (en) * | 2017-11-27 | 2018-02-02 | 衡阳市日升娃娃鱼养殖基地 | A kind of artificial breeding method of the giant salamander |
| CN107821319A (en) * | 2017-11-27 | 2018-03-23 | 衡阳市日升娃娃鱼养殖基地 | The artificial fecundation method of the giant salamander |
| CN107853246A (en) * | 2017-11-27 | 2018-03-30 | 衡阳市日升娃娃鱼养殖基地 | Giant salamander semen diluent, preparation method and its application method |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH08228631A (en) * | 1995-02-27 | 1996-09-10 | Kyowa Concrete Kogyo Kk | Nesting hole apparatus for spawning of fishes |
| CN103493783A (en) * | 2013-10-18 | 2014-01-08 | 梅县海霞水产品有限公司 | Method for improving ovum quality of giant salamander parent |
| CN103583464A (en) * | 2013-10-17 | 2014-02-19 | 王德奎 | Hasten parturition method for artificially bred giant salamanders |
| CN103918602A (en) * | 2014-04-17 | 2014-07-16 | 金寨县胜华娃娃鱼开发有限公司 | Efficient artificial breeding technology for giant salamanders |
-
2015
- 2015-03-10 CN CN201510103806.8A patent/CN104737977A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH08228631A (en) * | 1995-02-27 | 1996-09-10 | Kyowa Concrete Kogyo Kk | Nesting hole apparatus for spawning of fishes |
| CN103583464A (en) * | 2013-10-17 | 2014-02-19 | 王德奎 | Hasten parturition method for artificially bred giant salamanders |
| CN103493783A (en) * | 2013-10-18 | 2014-01-08 | 梅县海霞水产品有限公司 | Method for improving ovum quality of giant salamander parent |
| CN103918602A (en) * | 2014-04-17 | 2014-07-16 | 金寨县胜华娃娃鱼开发有限公司 | Efficient artificial breeding technology for giant salamanders |
Non-Patent Citations (6)
| Title |
|---|
| 乔志刚: ""中国大鲵繁殖生物学及人工繁殖技术研究进展"", 《动物学报》 * |
| 卞伟: ""大鲵Megalobatrachus davidinus（Blanchard）生物学及人工养殖技术"", 《现代渔业信息》 * |
| 卞伟: ""大鲵的生物学及养殖技术（三）"", 《科学养鱼》 * |
| 彭亮跃等: ""低温和超低温保存对中国大鲵成熟精子的影响"", 《水生生物学报》 * |
| 施学文: ""大鲵人工繁殖技术初步研究"", 《福建水产》 * |
| 金立成: ""大鲵规模化繁殖新技术"", 《河北渔业》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107646798A (en) * | 2017-11-27 | 2018-02-02 | 衡阳市日升娃娃鱼养殖基地 | A kind of artificial breeding method of the giant salamander |
| CN107821319A (en) * | 2017-11-27 | 2018-03-23 | 衡阳市日升娃娃鱼养殖基地 | The artificial fecundation method of the giant salamander |
| CN107853246A (en) * | 2017-11-27 | 2018-03-30 | 衡阳市日升娃娃鱼养殖基地 | Giant salamander semen diluent, preparation method and its application method |
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Application publication date: 20150701 |