CN101461341B - Induced spawning method of Brachymystax lenok - Google Patents
Induced spawning method of Brachymystax lenok Download PDFInfo
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- CN101461341B CN101461341B CN2009100712240A CN200910071224A CN101461341B CN 101461341 B CN101461341 B CN 101461341B CN 2009100712240 A CN2009100712240 A CN 2009100712240A CN 200910071224 A CN200910071224 A CN 200910071224A CN 101461341 B CN101461341 B CN 101461341B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
An artificial inducement method for spawning of brachymystax lenok, relates to an artificial inducement method for spawning of fish, which solves the problems of unfocused spawning time, long spawning period and low hatching rate, comprising the steps of: 1. respectively delivering the parent fish into an induced spawning pond in spring; 2. performing induced spawning on the parent fish when water temperature reaches 7-12 DEG C; 3. injecting oxytocic in the pectoral fin basis free of squamae after the male and the female fish are hocused, adopting the secondary injection, then mixing the parent fish, after seven days, discharging seminal fluid by the male fish and ovulating by the female fish, namely manual induced spawning of brachymystax lenok. The invention causes spawning time to centralize, the spawning time is shortened by 10-15 days, and the hatching rate reaches 60%-70%; manual induced spawning is performed to breed the parent fish, the induced spawning success rate of the male fish reaches 100%, and the induced spawning success rate of the female fish reaches 40-50%; manual induced spawning is performed on wild parent fish, the induced spawning success rate of the male fish reaches 100%, and the induced spawning success rate of the female fish reaches 50-80%.
Description
Technical field
The present invention relates to the induced spawning method of a kind of fish.
Background technology
Brachymystax lenok belongs to salmon section brachymystax lenok and belongs to cold water fishes; owing to reasons such as economical activities of mankind, overfishings; population reduces the brachymystax lenok nature in a large number; endangered; be listed in national second class protection aqua-marine life; through artificial propagation and breed, improve population quantity now, effectively protected this species.After brachymystax lenok reaches sexual maturity, relying on water temperature variation and current to stimulate lays eggs, have and take off the habit that the ovum hatching is buried in the hole, but egg-laying time is not concentrated, oviposition period is longer, fish-egg can't collect and incubation rate low, only be 30～40%, and frequently making the parent population bodily injury serious to the maturity inspection of parent population, multiple batches of laying eggs concentrated also for hatching management and fry to culture and brought very big inconvenience.
Summary of the invention
The objective of the invention is to have the problem that egg-laying time is not concentrated, oviposition period is long and incubation rate is low in order to solve existing brachymystax lenok, and the induced spawning method of a kind of brachymystax lenok that proposes.
The induced spawning method of brachymystax lenok is realized according to the following steps: one, in annual spring, sexually matured parent population is invested in respectively in the spawning inducing pool, spawning inducing pool is a flowing water, and the depth of water is 50～60cm, and water temperature is 6 ℃, and spawning inducing pool afterbody draining dissolved oxygen amount is 5～10mg/L; Two, carrying out parent population when water temperature reaches 7～12 ℃ hastens parturition, 1h preparation oxytocic drug before hastening parturition, human chorionic gonadtropin (HCG), the salmon releasing hormone analog (S-GnRH-A) of 10ug and the maleic acid DOM (DOM) of 2mg of per kilogram raun injection 1000IU, the milter injected dose reduces by half; Three, male and female parent population anesthesia back does not have squama place injection oxytocic drug at the pectoral fin base portion, inject for the first time 20% of oxytocic drug, inject for the second time 80% of oxytocic drug, the time interval of double injection is 48～52h, by 3: 1～3 male and female ratio that the parent population in the spawning inducing pool is admixed together again, the artificial induced spawning of brachymystax lenok is promptly finished in smart, the raun ovulation of milter row after 7 days; Wherein in the step 1 sexually matured parent population be 3 ages above propagate artificially parent population or 4 ages above wild parent population.
The present invention brachymystax lenok of manually hastening parturition, the double injection of oxytocic drug has increased the metabolic rate of parent population, colony's gonad development is neat, changed the spawning habit of brachymystax lenok, egg-laying time is concentrated, brought convenience for hatching, fry breed, sale, reduce hold facility, save manpower, oviposition period has shortened 10～15 days simultaneously, and incubation rate is up to 60%～70%; The present invention manually hastens parturition and cultures parent population, and the success rate of hastening parturition of milter reaches 100%, and the success rate of hastening parturition of raun reaches 40%～50%; The wild parent population of artificial induced spawning, the success rate of hastening parturition of milter reaches 100%, and the success rate of hastening parturition of raun reaches 50%～80%.
Embodiment
Technical solution of the present invention is not limited to following cited embodiment, also comprises any combination between each embodiment.
Embodiment one: the induced spawning method of present embodiment brachymystax lenok is realized according to the following steps: one, in annual spring, be invested in sexually matured parent population in the spawning inducing pool respectively, spawning inducing pool is a flowing water, the depth of water is 50～60cm, water temperature is 6 ℃, and spawning inducing pool afterbody draining dissolved oxygen amount is 5～10mg/L; Two, when water temperature reaches 7～12 ℃, carry out parent population and hasten parturition, 1h preparation oxytocic drug before hastening parturition, human chorionic gonadtropin, the salmon releasing hormone analog of 10ug and the maleic acid DOM of 2mg of per kilogram raun injection 1000IU, the milter injected dose reduces by half; Three, male and female parent population anesthesia back does not have squama place injection oxytocic drug at the pectoral fin base portion, inject for the first time 20% of oxytocic drug, inject for the second time 80% of oxytocic drug, the time interval of double injection is 48～52h, by 3: 1～3 male and female ratio that the parent population in the spawning inducing pool is admixed together again, the artificial induced spawning of brachymystax lenok is promptly finished in smart, the raun ovulation of milter row after 7 days; Wherein in the step 1 sexually matured parent population be 3 ages above propagate artificially parent population or 4 ages above wild parent population.
Sexually matured parent population in the present embodiment step 1, the parent population in 3～4 ages rely on gonopore to judge male and female, and female outstanding, red and swollen for gonopore, male is the gonopore indent; The parent population that 5 ages are above, female for belly expand soft, gonopore outstanding, red and swollen, malely be the body surface blackening and have erythema.
The spawning inducing pool top adds cover net in the present embodiment step 1, prevents the parent population spawning inducing pool that jumps out.
Embodiment two: what present embodiment and embodiment one were different is that the depth of water is 52～58cm in the step 1, and spawning inducing pool afterbody draining dissolved oxygen amount is 6～8mg/L.Other step and parameter are identical with embodiment one.
Embodiment three: what present embodiment and embodiment one were different is that the depth of water is 55cm in the step 1, and spawning inducing pool afterbody draining dissolved oxygen amount is 7mg/L.Other step and parameter are identical with embodiment one.
Embodiment four: what present embodiment and embodiment one, two or three were different is to carry out parent population in the step 2 to hasten parturition when water temperature reaches 8～11 ℃.Other step and parameter are identical with embodiment one, two or three.
Embodiment five: what present embodiment and embodiment one, two or three were different is to carry out parent population in the step 2 to hasten parturition when water temperature reaches 10 ℃.Other step and parameter are identical with embodiment one, two or three.
Embodiment six: present embodiment and embodiment four are different is that time interval of double injection in the step 3 is 49～51h.Other step and parameter are identical with embodiment four.
Embodiment seven: present embodiment and embodiment five are different is that time interval of double injection in the step 3 is 50h.Other step and parameter are identical with embodiment five.
Embodiment eight: what present embodiment was different with embodiment six or seven is that the male and female ratio by 3: 1～2 is admixed together with the parent population in the spawning inducing pool in the step 3.Other step and parameter are identical with embodiment six or seven.
Embodiment nine: what present embodiment was different with embodiment six or seven is that the male and female ratio by 3: 1 is admixed together with the parent population in the spawning inducing pool in the step 3.Other step and parameter are identical with embodiment six or seven.
Embodiment ten: what present embodiment was different with embodiment six or seven is that the male and female ratio by 3: 2 is admixed together with the parent population in the spawning inducing pool in the step 3.Other step and parameter are identical with embodiment six or seven.
Embodiment 11: what present embodiment was different with embodiment six or seven is that the male and female ratio by 3: 3 is admixed together with the parent population in the spawning inducing pool in the step 3.Other step and parameter are identical with embodiment six or seven.
Embodiment 12: the induced spawning method of present embodiment brachymystax lenok is realized according to the following steps: one, in annual spring, sexually matured parent population is invested in respectively in the spawning inducing pool, and spawning inducing pool is a flowing water, and the depth of water is 55cm, water temperature is 6 ℃, and spawning inducing pool afterbody draining dissolved oxygen amount is 8mg/L; Two, when water temperature reaches 10 ℃, carry out parent population and hasten parturition, 1h preparation oxytocic drug before hastening parturition, human chorionic gonadtropin, the salmon releasing hormone analog of 10ug and the maleic acid DOM of 2mg of per kilogram raun injection 1000IU, the milter injected dose reduces by half; Three, male and female parent population anesthesia back does not have squama place injection oxytocic drug at the pectoral fin base portion, inject for the first time 20% of oxytocic drug, inject for the second time 80% of oxytocic drug, the time interval of double injection is 48h, by 3: 2 male and female ratio that the parent population in the spawning inducing pool is admixed together again, the artificial induced spawning of brachymystax lenok is promptly finished in smart, the raun ovulation of milter row after 7 days; Wherein in the step 1 sexually matured parent population be 3 ages above propagate parent population artificially.
Artificial induced spawning is cultured parent population in the present embodiment, and the success rate of hastening parturition of milter reaches 100%, and the success rate of hastening parturition of raun reaches 50%, and incubation rate is up to 68%.
Embodiment 13: the induced spawning method of present embodiment brachymystax lenok is realized according to the following steps: one, in annual spring, sexually matured parent population is invested in respectively in the spawning inducing pool, and spawning inducing pool is a flowing water, and the depth of water is 55cm, water temperature is 6 ℃, and spawning inducing pool afterbody draining dissolved oxygen amount is 8mg/L; Two, when water temperature reaches 10 ℃, carry out parent population and hasten parturition, 1h preparation oxytocic drug before hastening parturition, human chorionic gonadtropin, the salmon releasing hormone analog of 10ug and the maleic acid DOM of 2mg of per kilogram raun injection 1000IU, the milter injected dose reduces by half; Three, male and female parent population anesthesia back does not have squama place injection oxytocic drug at the pectoral fin base portion, inject for the first time 20% of oxytocic drug, inject for the second time 80% of oxytocic drug, the time interval of double injection is 50h, by 3: 2 male and female ratio that the parent population in the spawning inducing pool is admixed together again, the artificial induced spawning of brachymystax lenok is promptly finished in smart, the raun ovulation of milter row after 7 days; Wherein sexually matured parent population is above wild parent populations in 4 ages in the step 1.
The wild parent population of artificial induced spawning in the present embodiment, the success rate of hastening parturition of milter reaches 100%, and the success rate of hastening parturition of raun reaches 80%, and incubation rate is up to 70%.
Claims (10)
1. the induced spawning method of a brachymystax lenok, the induced spawning method that it is characterized in that brachymystax lenok is realized according to the following steps: one, in annual spring, be invested in sexually matured parent population in the spawning inducing pool respectively, spawning inducing pool is a flowing water, the depth of water is 50～60cm, water temperature is 6 ℃, and spawning inducing pool afterbody draining dissolved oxygen amount is 5～10mg/L; Two, when water temperature reaches 7～12 ℃, carry out parent population and hasten parturition, 1h preparation oxytocic drug before hastening parturition, the human chorionic gonadtropin of per kilogram raun injection 1000IU, the salmon of 10ug discharge the maleic acid DOM of S-GnRH-A and 2mg, and the milter injected dose reduces by half; Three, male and female parent population anesthesia back does not have squama place injection oxytocic drug at the pectoral fin base portion, inject for the first time 20% of oxytocic drug, inject for the second time 80% of oxytocic drug, the time interval of double injection is 48～52h, by 3: 1～3 male and female ratio that the parent population in the spawning inducing pool is admixed together again, the artificial induced spawning of brachymystax lenok is promptly finished in smart, the raun ovulation of milter row after 7 days; Wherein in the step 1 sexually matured parent population be 3 ages above propagate artificially parent population or 4 ages above wild parent population.
2. the induced spawning method of a kind of brachymystax lenok according to claim 1 is characterized in that the depth of water is 52～58cm in the step 1, and spawning inducing pool afterbody draining dissolved oxygen amount is 6～8mg/L.
3. the induced spawning method of a kind of brachymystax lenok according to claim 1 is characterized in that the depth of water is 55cm in the step 1, and spawning inducing pool afterbody draining dissolved oxygen amount is 7mg/L.
4. according to the induced spawning method of claim 1,2 or 3 described a kind of brachymystax lenoks, it is characterized in that when water temperature reaches 8～11 ℃, carrying out in the step 2 parent population and hasten parturition.
5. according to the induced spawning method of claim 1,2 or 3 described a kind of brachymystax lenoks, it is characterized in that when water temperature reaches 10 ℃, carrying out in the step 2 parent population and hasten parturition.
6. the induced spawning method of a kind of brachymystax lenok according to claim 4, the time interval that it is characterized in that double injection in the step 3 is 49～51h.
7. the induced spawning method of a kind of brachymystax lenok according to claim 5, the time interval that it is characterized in that double injection in the step 3 is 50h.
8. according to the induced spawning method of claim 6 or 7 described a kind of brachymystax lenoks, it is characterized in that the male and female ratio by 3: 1～2 is admixed together with the parent population in the spawning inducing pool in the step 3.
9. according to the induced spawning method of claim 6 or 7 described a kind of brachymystax lenoks, it is characterized in that the male and female ratio by 3: 1 is admixed together with the parent population in the spawning inducing pool in the step 3.
10. according to the induced spawning method of claim 6 or 7 described a kind of brachymystax lenoks, it is characterized in that the male and female ratio by 3: 2 is admixed together with the parent population in the spawning inducing pool in the step 3.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009100712240A CN101461341B (en) | 2009-01-08 | 2009-01-08 | Induced spawning method of Brachymystax lenok |
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| CN2009100712240A CN101461341B (en) | 2009-01-08 | 2009-01-08 | Induced spawning method of Brachymystax lenok |
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| CN101461341A CN101461341A (en) | 2009-06-24 |
| CN101461341B true CN101461341B (en) | 2011-08-17 |
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Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102599095B (en) * | 2012-04-12 | 2014-05-21 | 玉龙县龙泉水产养殖科技有限责任公司 | Estrus and spawning induction method for increasing rate of artificial insemination of Schizothorax davidi (Sauvage) |
| CN102599094B (en) * | 2012-04-12 | 2014-01-15 | 玉龙县龙泉水产养殖科技有限责任公司 | Method for artificial incubation of Percocypris pingi pingi |
| CN102657123A (en) * | 2012-05-22 | 2012-09-12 | 东北农业大学 | Artificial propagation method for Thymallus arcticusgrubei Dybowski |
| CN103518649B (en) * | 2012-07-06 | 2016-12-21 | 广州市番禺区农业科学研究所 | Fresh water black porgy and Jade Perch hybridizing propagation process |
| CN102715118B (en) * | 2012-07-17 | 2013-07-31 | 中国水产科学研究院黑龙江水产研究所 | Method applicable to all-female diploid breeding of trouts |
| CN103348947B (en) * | 2013-07-31 | 2015-02-18 | 河北省海洋与水产科学研究院 | Method for reducing postpartum mortality of brachymystax lenok parent fish |
| CN103960188B (en) * | 2014-05-20 | 2016-03-30 | 中国水产科学研究院长江水产研究所 | A kind of long Qi Wen Minnow induced spawning method |
| CN104585093B (en) * | 2015-01-14 | 2016-08-31 | 成都市天府新区丹龙渔业养殖专业合作社 | A kind of method of artificial induced spawning fish-egg |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1376382A (en) * | 2001-03-27 | 2002-10-30 | 围场满族蒙古族自治县水产技术推广站 | Technique for culturing fine-scale fish in fishpond |
-
2009
- 2009-01-08 CN CN2009100712240A patent/CN101461341B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1376382A (en) * | 2001-03-27 | 2002-10-30 | 围场满族蒙古族自治县水产技术推广站 | Technique for culturing fine-scale fish in fishpond |
Non-Patent Citations (4)
| Title |
|---|
| 徐伟等.哲罗鱼人工育苗技术研究.《上海水产大学学报》.2008,第17卷(第4期),第452－456页. * |
| 徐伟等.哲罗鱼全人工繁育的初步研究.《中国水产科学》.2007,第14卷(第6期),第896-900页. * |
| 徐革锋等.尖吻和钝吻细鳞鱼（Brachymystax Lenok）精子活力的观察.《水产学杂志》.2005,第18卷(第2期),第16－21页. * |
| 韩英等.黑龙江茴鱼的人工繁殖.《科学养鱼》.2008,(第1期),第6-8页. * |
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