CN108184727A - A kind of artificial fecundation method of the former carp of crow - Google Patents
A kind of artificial fecundation method of the former carp of crow Download PDFInfo
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- CN108184727A CN108184727A CN201810239371.3A CN201810239371A CN108184727A CN 108184727 A CN108184727 A CN 108184727A CN 201810239371 A CN201810239371 A CN 201810239371A CN 108184727 A CN108184727 A CN 108184727A
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- 241000252233 Cyprinus carpio Species 0.000 title claims abstract description 73
- 238000000034 method Methods 0.000 title claims abstract description 53
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 69
- 230000012447 hatching Effects 0.000 claims abstract description 48
- 235000013601 eggs Nutrition 0.000 claims abstract description 46
- 241000251468 Actinopterygii Species 0.000 claims abstract description 32
- 239000007924 injection Substances 0.000 claims abstract description 19
- 238000002347 injection Methods 0.000 claims abstract description 19
- 230000032696 parturition Effects 0.000 claims abstract description 19
- 230000000384 rearing effect Effects 0.000 claims abstract description 18
- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 17
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 17
- 210000004681 ovum Anatomy 0.000 claims abstract description 17
- 230000017448 oviposition Effects 0.000 claims abstract description 15
- 230000009027 insemination Effects 0.000 claims abstract description 14
- 210000000582 semen Anatomy 0.000 claims abstract description 6
- 239000000203 mixture Substances 0.000 claims abstract description 3
- 235000019688 fish Nutrition 0.000 claims description 33
- 210000001015 abdomen Anatomy 0.000 claims description 14
- 241000252210 Cyprinidae Species 0.000 claims description 13
- 239000002504 physiological saline solution Substances 0.000 claims description 10
- 238000009395 breeding Methods 0.000 claims description 7
- 230000001488 breeding effect Effects 0.000 claims description 7
- 230000035800 maturation Effects 0.000 claims description 7
- 230000004720 fertilization Effects 0.000 claims description 6
- 239000013505 freshwater Substances 0.000 claims description 6
- 239000012528 membrane Substances 0.000 claims description 6
- 230000002710 gonadal effect Effects 0.000 claims description 5
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 4
- 230000037396 body weight Effects 0.000 claims description 4
- 150000001875 compounds Chemical class 0.000 claims description 4
- 229940046085 endocrine therapy drug gonadotropin releasing hormone analogues Drugs 0.000 claims description 4
- XLXSAKCOAKORKW-AQJXLSMYSA-N gonadorelin Chemical class C([C@@H](C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N1[C@@H](CCC1)C(=O)NCC(N)=O)NC(=O)[C@H](CO)NC(=O)[C@H](CC=1C2=CC=CC=C2NC=1)NC(=O)[C@H](CC=1N=CNC=1)NC(=O)[C@H]1NC(=O)CC1)C1=CC=C(O)C=C1 XLXSAKCOAKORKW-AQJXLSMYSA-N 0.000 claims description 4
- 230000001817 pituitary effect Effects 0.000 claims description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 3
- 239000002131 composite material Substances 0.000 claims description 3
- 238000007912 intraperitoneal administration Methods 0.000 claims description 3
- 150000002689 maleic acids Chemical class 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 claims description 3
- 229910052760 oxygen Inorganic materials 0.000 claims description 3
- 239000001301 oxygen Substances 0.000 claims description 3
- 210000000006 pectoral fin Anatomy 0.000 claims description 3
- 238000005096 rolling process Methods 0.000 claims description 3
- 108010044781 salmon gonadotropin Proteins 0.000 claims description 3
- 230000000694 effects Effects 0.000 claims description 2
- 150000002576 ketones Chemical class 0.000 claims 1
- 238000003756 stirring Methods 0.000 claims 1
- 230000004083 survival effect Effects 0.000 abstract description 6
- 238000011031 large-scale manufacturing process Methods 0.000 abstract description 5
- 230000035558 fertility Effects 0.000 abstract description 4
- 239000003814 drug Substances 0.000 abstract description 3
- 229940079593 drug Drugs 0.000 abstract description 3
- 230000002196 ecbolic effect Effects 0.000 abstract description 3
- 230000026109 gonad development Effects 0.000 abstract description 2
- 230000008569 process Effects 0.000 description 14
- 230000001568 sexual effect Effects 0.000 description 4
- ORTYMGHCFWKXHO-UHFFFAOYSA-N diethadione Chemical compound CCC1(CC)COC(=O)NC1=O ORTYMGHCFWKXHO-UHFFFAOYSA-N 0.000 description 3
- 229960003675 diethadione Drugs 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- 210000004907 gland Anatomy 0.000 description 2
- 210000001672 ovary Anatomy 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 241000972773 Aulopiformes Species 0.000 description 1
- 241000252206 Cypriniformes Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241001489469 Procypris Species 0.000 description 1
- 241001290088 Procypris mera Species 0.000 description 1
- OFOBLEOULBTSOW-UHFFFAOYSA-N Propanedioic acid Natural products OC(=O)CC(O)=O OFOBLEOULBTSOW-UHFFFAOYSA-N 0.000 description 1
- 208000026487 Triploidy Diseases 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 230000008033 biological extinction Effects 0.000 description 1
- 244000144987 brood Species 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- VZCYOOQTPOCHFL-UPHRSURJSA-N maleic acid Chemical compound OC(=O)\C=C/C(O)=O VZCYOOQTPOCHFL-UPHRSURJSA-N 0.000 description 1
- 239000011976 maleic acid Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000004080 punching Methods 0.000 description 1
- 235000019515 salmon Nutrition 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- VZCYOOQTPOCHFL-UHFFFAOYSA-N trans-butenedioic acid Natural products OC(=O)C=CC(O)=O VZCYOOQTPOCHFL-UHFFFAOYSA-N 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- 238000003911 water pollution Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/17—Hatching, e.g. incubators
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses a kind of artificial fecundation method of the former carp of crow, wherein, the artificial fecundation method includes：Parent fish rearing：The former carp of of the right age crow is selected to be fed；Parent population selects：Select body and the former carp of the good crow of gonad development；It hastens parturition：Injection oxytocic drug is carried out to the former carp of the crow of selection to hasten parturition；Artificial insemination：Parent population after hastening parturition is put into spawning pond, bath makes oviposition water form flow, in parent population heat climax and when starting oviposition, carries out adopting ovum and semen collection, the ripe ovum and essence of acquisition are placed in container and gently mix completion insemination, forms fertilized eggs；Unsticking：Unsticking is carried out to fertilized eggs using yellow mud water；Hatching：Using two sections of hatching methods.In embodiments of the present invention, the fish-egg fertility rate and hatchability of black former carp is improved, the survival rate of fry realizes the large-scale production of the former carp fry of crow, improves the success rate of black former carp artificial propagation, reduces the cultivation quantity of the former carp parent population of crow, reduce producing cost.
Description
Technical field
The present invention relates to technical field of aquaculture more particularly to a kind of artificial fecundation methods of the former carp of crow.
Background technology
Black original carp (Procypris merus) belongs to Cypriniformes, Cyprinidae, Procypris.On being distributed in each main rivers in Guangxi
Trip is Zhujiang River Endemic fish.In recent years, rivers caused by the water development of Guangxi rivers step obstruct, and water pollution and transition are caught
The factors such as fishing, serious destruction is caused to River Fish resource.The natural resources of black original carp is very deficient, it would be highly desirable to protect, arrange
Enter《Chinese animals on the brink of extinction Red Data Book》(1998) endangered species register (susceptible species).
The breeding of Chinese Cyprinidae cultured fishes generally using artificial propagation physiological ecological method, whole process divide parent fish rearing,
Promote the sexual maturity, inseminate and hatch 4 links.1. parent fish rearing.It is to be up to the parent fish rearing of age at sexual maturity to gonadal maturation
Process.2. it hastens parturition.Occur the parent population of above-mentioned character to ovary, ocyodinic must be injected and promote its heat, oviposition.3. it inseminates.Have certainly
So two kinds of insemination and artificial insemination.Naturally insemination is that the parent population after hastening parturition voluntarily lays eggs in spawning pond, arranges essence, completes fertilization work
With.Artificial insemination is when parent population heat climax will lay eggs, and carries out adopting ovum, semen collection, and ripe essence, ovum is made to be completed in vessel
Spermatization.4. hatch.Fertilized eggs are placed in the tools such as circular hatching channal, hatching barrel and are carried out.
The artificial propagation of the former carp of crow is implemented using the artificial propagation of fish method of above routine, there are fish-egg rate of fertilization is low,
The problem of easy infection water mo(u)ld, hatching rate is low, and survival rate of fish fry is low, it is difficult to mass produce fry.
In this case, by strengthening parent fish rearing management, mature brood fish is selected, injects suitable estrualization agent and dosage
Promote its heat, oviposition, rate of fertilization is improved using artificial insemination, unsticking, adjust the flow velocity of incubator water, prevention egg membrane is early broken, can
To realize the large-scale production of fry and fingerling.
Invention content
It is an object of the invention to overcome the deficiencies in the prior art, and the present invention provides a kind of artificial propagation sides of the former carp of crow
Method, improves the fish-egg fertility rate and hatchability of black former carp, and the survival rate of fry is realized the large-scale production of the former carp fry of crow, carried
The high success rate of black former carp artificial propagation, reduces the cultivation quantity of the former carp parent population of crow, reduces producing cost.
In order to solve the above-mentioned technical problem, the present invention implements the artificial fecundation method for providing a kind of former carp of crow, described
Artificial fecundation method includes：
Parent fish rearing：The 5 former carps of male crow more than age and the 6 former carp of female crow more than age is selected to be put into parent fish rearing pond
Interior, parent fish rearing pool area is 1500-2000m2, depth of water 1.0-1.5m, breeding density 667m2The water surface is no more than 150kg, male and female
The ratio of stocking of parent population is 10 ﹕ 1-2, feeds the compound feed for carps of protein content 30-35%, and day feeding volume is fish body weight
3-4% is respectively fed once sooner or later, and in the artificial propagation previous moon, feeding volume is kept to original half, and every 7-10 days
The new water of punching note 1 time promotes the former carp gonadal maturation of crow；
Parent population selects：Full body surface is selected to have harsh feeling, abdomen expands in shape, lower abdomen is soft, cloacal aperture is ruddy
The former carp of female crow；Selection whole body body surface has harsh feeling, there is the milky sperm outflow scattered into water when slighter compress abdomen
The former carp of male crow, the male and female combo ratio for selecting the parent population for hastening parturition is 4-5:1；
It hastens parturition：To the selected parent population hastened parturition, disposable injection ocyodinic is carried out, promotes parent population heat, oviposition and production essence, it is right
The dosage of female parent population injection ocyodinic is 1.0ml/kg, and the dosage to male parent population injection ocyodinic is 0.5ml/kg, wherein,
Ocyodinic per 1.0ml is by 0.2mg carps pituitary, the salmon gonadotropin releasing hormone analogues of 10 μ g, 3mg maleic acids
Dioxone and 0.7% physiological saline are configured；
Artificial insemination：Parent population after hastening parturition is put into spawning pond, bath makes oviposition water form flow, during flow effect
Between be 16-24h；In parent population heat climax and when starting oviposition, carry out adopting ovum and semen collection, the ripe ovum and essence of acquisition are placed on
In same container, and 50-100ml physiological saline is added in container, gently mix completion insemination, form fertilized eggs；
Unsticking：Diluted yellow mud water is put into washbowl, fertilized eggs are poured into yellow mud water and are gently mixed slowly, make by
Smart ovum is in close contact with yellow mud water, and yellow mud is sticked to fertilized eggs surface and completes unsticking, clear water is used to rinse fertilized eggs after the completion of unsticking；
Hatching：Fertilized eggs are hatched using two sections of hatching methods.
Preferably, described is from parent population pectoral fin base using syringe to female parent population and male parent population injection ocyodinic
45 ° of injection parent populations of recess inclination are intraperitoneal on rear side of portion, and needle point is pierced into 1.0cm in parent population body.
Preferably, the diluted yellow mud water is stirred by the proportion composite of 1 part of particulate yellow mud and 7 parts of water.
Preferably, it is described that fertilized eggs are hatched using two sections of hatching methods, including：
Hatch early period, mid-term：Fertilized eggs are put into hatching barrel, using hatching barrel Lotic hatching, flow is controlled, makes fertilized eggs
Slowly rolling from bottom to top；
Hatch the later stage：Fertilized eggs are moved into tablet incubator and carry out micro- Lotic hatching, are lain on one's side after making fry membrane at the bottom.
Preferably, the hatching water temperature is 16 DEG C to 22 DEG C, and the brooding time is 150h to 80h.
Preferably, the Spawning water is greater than or equal to the limpid fresh water of 6mg/L, the temperature difference no more than 2 DEG C for dissolved oxygen amount,
The limpid fresh water meets the regulation of water quality standard for fishery (GB11607).
Preferably, the triploidy rate that the hatching barrel is put into is to be put into no more than 50,000 fertilized eggs per 100L water bodys.
Preferably, the tablet incubator is flat pond or Flat bottom container.
Preferably, period of the hatching later stage for 1-8h before fry membrane.
The physiological ecological method that the former carp artificial fecundation method of crow of the invention uses, in addition unsticking and two sections of hatching methods, improve
The fish-egg fertility rate and hatchability of black original carp, the survival rate of fry realize the large-scale production of the former carp fry of crow；Improve black former carp
The success rate of artificial propagation reduces the cultivation quantity of the former carp parent population of crow, reduces producing cost；It reduces to black former carp wild resource
It fishes and destroys.
Description of the drawings
In order to illustrate more clearly about the embodiment of the present invention or technical scheme of the prior art, to embodiment or will show below
There is attached drawing needed in technology description to be briefly described, it is clear that, the accompanying drawings in the following description is only this
Some embodiments of invention, for those of ordinary skill in the art, without creative efforts, can be with
Other attached drawings are obtained according to these attached drawings.
Fig. 1 is the flow diagram of the artificial fecundation method of the former carp of crow in the embodiment of the present invention.
Specific embodiment
Below in conjunction with the attached drawing in the embodiment of the present invention, the technical solution in the embodiment of the present invention is carried out clear, complete
Site preparation describes, it is clear that described embodiment is only part of the embodiment of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, those of ordinary skill in the art are obtained all other without creative efforts
Embodiment shall fall within the protection scope of the present invention.
During black former carp artificial propagation, it is usually required mainly for select, hasten parturition by parent fish rearing, parent population, being fertilized, unsticking and
Hatch 6 links.
Fig. 1 is the flow diagram of the artificial fecundation method of the former carp of crow in the embodiment of the present invention, as shown in figure, described
Artificial fecundation method includes：
S11：Parent fish rearing：The 5 former carps of male crow more than age and the 6 former carp of female crow more than age is selected to be put into parent fish rearing
In pond, parent fish rearing pool area is 1500-2000m2, depth of water 1.0-1.5m, breeding density 667m2The water surface is no more than 150kg, female
The ratio of stocking of male parent population is 10 ﹕ 1-2, feeds the compound feed for carps of protein content 30-35%, and day feeding volume is fish body weight
3-4%, respectively feed sooner or later primary, in the artificial propagation previous moon, feeding volume is kept to original half, and every 7-10
The new water of Tianchong note 1 time promotes the former carp gonadal maturation of crow；
In specific implementation process of the present invention, parent fish rearing is to be up to the parent fish rearing of age at sexual maturity to gonad development
Ripe process；Black original carp maturation age：The male former carp of crow is 5 more than age, the former carp of female crow is 6 more than age；Therefore 5 ages of selection
The above former carp of male crow and the 6 former carp of female crow more than age, which are put into parent fish rearing pond, to be cultivated, parent fish rearing pool area
In 1500-2000m2, for the water depth of water between 1.0-1.5m, crow original carp breeding density is per 667m2The water surface is no more than 15kg, female
The ratio of stocking of male parent population is 10：1-2；In cultivating process, the compound feed for carps of protein content 30-35%, day are fed
Feeding volume is the 3-4% of fish body weight, is respectively fed sooner or later once, in the artificial propagation previous moon, feeding volume is kept to original two points
One of, and it is primary every the new water of 7-10 Tianchongs note, promote the gonadal maturation of the former carp of crow.
S12：Parent population selects：Full body surface is selected to have harsh feeling, abdomen expands in shape, lower abdomen is soft, cloacal aperture
The ruddy former carp of female crow；Selection whole body body surface has harsh feeling, there is the milky sperm to scatter into water when slighter compress abdomen
The former carp of male crow of outflow, the male and female combo ratio for selecting the parent population for hastening parturition is 4-5:1；
In specific implementation process of the present invention, in order to ensure the success rate of artificial propagation, in parent population selection, male crow is former
Carp selects full body surface to have harsh feeling, there is the milky sperm outflow scattered into water when slighter compress abdomen, and female crow is former
Sexual gland physically well develops, there is harsh feeling on whole body surface, abdomen expands in shape, lower abdomen is soft, cloacal aperture is ruddy for carp selection；It will
Selected parent population is for preparation of hastening parturition, and the former carp of female crow selected and the combo ratio of the former carp of male crow are 4-5:1.
S13：It hastens parturition：To the selected parent population hastened parturition, disposable injection ocyodinic is carried out, promotes parent population heat, oviposition and production
Essence, the dosage to female parent population injection ocyodinic is 1.0ml/kg, and the dosage to male parent population injection ocyodinic is 0.5ml/kg,
Wherein, the ocyodinic per 1.0ml is by 0.2mg carps pituitary, salmon gonadotropin releasing hormone analogues, the 3mg of 10 μ g
Maleic acid dioxone and 0.7% physiological saline are configured；
In specific implementation process of the present invention, occur that gland physically well develops, there is harsh feeling on whole body surface, in shape to ovary
The former carp parent population of female crow that abdomen expands, lower abdomen is soft, cloacal aperture is ruddy, needs to inject ocyodinic, promotes its heat, oviposition；
The ocyodinic of every kilogram of female parent population injection 1.0ml, wherein salmon of the ocyodinic of every 1.0ml by 0.2mg carps pituitary, 10 μ g
Fish gonadotropin releasing hormone analogues, 3mg maleic acids dioxone and 0.7% physiological saline are configured, to female parent
The injection volume of fish is every kilogram of 1.0ml, is every kilogram of 0.5ml to male parent population injection volume.
Wherein, it is to be inclined using syringe from recess on rear side of parent population pectoral fin base portion to female, male parent population injection ocyodinic
Oblique 45 ° of injections parent population is intraperitoneal, and needle point is pierced into 1.0cm in parent population body.
S14：Artificial insemination：Parent population after hastening parturition is put into spawning pond, bath makes oviposition water form flow, in parent population
Heat climax and when starting oviposition, carries out adopting ovum and semen collection, the ripe ovum and essence of acquisition is placed in same container, and holding
50-100ml physiological saline is added in device, completion insemination is gently mixed, forms fertilized eggs；
In specific implementation process of the present invention, the parent population after injection ocyodinic is uniformly put into spawning pond, in spawning pond
Middle bath so that spawning pond forms certain flow rate of water flow, parent population heat climax and start oviposition when, respectively to parent population carry out
Ovum and semen collection are adopted, and the ovum of collected maturation and sperm are put into container, adds in appropriate physiological saline, i.e., and in container
Interior addition 50-100ml physiological saline, is gently mixed, fertilization is rapidly completed, fertilized eggs are formed, because ovum and sperm are in fresh water
The time of middle survival is shorter, so artificial insemination is rapidly completed.
Wherein, during the experiment, when the amount for finding the physiological saline added in is 100ml, rate of fertilization highest.
S15：Unsticking：Diluted yellow mud water is put into washbowl, fertilized eggs are poured into yellow mud water and are gently mixed slowly,
Be in close contact fertilized eggs and yellow mud water, yellow mud sticks and unsticking is completed on fertilized eggs surface, after the completion of unsticking using clear water rinse by
Smart ovum；
In specific implementation process of the present invention, during unsticking, unsticking is carried out using dilution yellow mud water, by diluted Huang
Muddy water is put into basin (washbowl etc.), is then poured into fertilized eggs in yellow mud water, and be gently mixed slowly so that fertilized eggs and Huang
Muddy water be in close contact, the yellow mud in yellow mud water stick fertilized eggs surface complete unsticking, after the completion of unsticking, with clear water rinse by
Smart ovum.
Wherein diluted yellow mud water is stirred by the proportion composite of 1 part of particulate yellow mud and 7 parts of water, number here
Refer to weight.
S16：Hatching：Fertilized eggs are hatched using two sections of hatching methods.
In specific implementation process of the present invention, specific hatching method is that fertilized eggs are incubated using two sections of hatching methods
Change；First stage includes hatching early period, mid-term, and second stage includes the hatching later stage.
Wherein hatch early period, mid-term：Fertilized eggs are put into hatching barrel, using hatching barrel Lotic hatching, control hatching barrel
In flow so that fertilized eggs from bottom to top slowly roll.
Hatch the later stage：Fertilized eggs are moved in tablet incubator and are hatched, lies on one's side, prevents at the bottom after making fry membrane
Fry is injured dead with flow rolling, improves hatching rate.
Wherein, in hatching process, the water temperature of hatching should be maintained at 16 DEG C to 22 DEG C, and brooding time is in 150h to 80h
Between, in suitable water temperature range, water temperature is higher, and the time of hatching is shorter.
Wherein, in hatching process, the oxygen content of Spawning water should be greater than or equal to 6mg/L, and the temperature difference of water temperature should not
It is advisable more than 2 DEG C, and Spawning water is limpid fresh water, which meets water quality standard for fishery
(GB11607) regulation.
Wherein, the density that fertilized eggs are placed in each hatching barrel should be no more than 50,000 fertilized eggs per 100L water bodys.
Wherein, tablet incubator should be flat pond or other Flat bottom containers.
Wherein, the hatching later stage refers to the period between the 1-8 hours before fry membrane.
In specific implementation process, because each fish is to different, the dosage needed of the susceptibility of oxytocic drug
It is also completely inconsistent, selects suitable oxytocic drug and dosage, black former carp parent population can be caused to synchronize sexal maturity, improve people
The success rate of work breeding.
In specific implementation process, because black former carp breeding during in early spring season, water temperature is relatively low, needs longer hatching
Time, fertilized eggs easily infect water mo(u)ld, and multiple fertilized eggs of non-unsticking are adhered together, it is easier to infect water mo(u)ld so that
Hatching rate substantially reduces；The hatchability of fertile eggs of non-unsticking is less than 30%, however the hatching rate of the fertilized eggs after unsticking can reach
90%, substantially increase hatching rate.
Following table is the former carp artificial propagation test's situation list of crow.
Black original carp artificial propagation test's situation list
The physiological ecological method that the former carp artificial fecundation method of crow of the invention uses, in addition unsticking and two sections of hatching methods, improve
The fish-egg fertility rate and hatchability of black original carp, the survival rate of fry realize the large-scale production of the former carp fry of crow；Improve black former carp
The success rate of artificial propagation reduces the cultivation quantity of the former carp parent population of crow, reduces producing cost；It reduces to black former carp wild resource
It fishes and destroys.
In addition, the artificial fecundation method of the former carp of a kind of crow provided above the embodiment of the present invention has carried out detailed Jie
It continues, specific case should be employed herein, the principle of the present invention and embodiment are expounded, the explanation of above example
It is merely used to help understand the method and its core concept of the present invention；Meanwhile for those of ordinary skill in the art, according to this
The thought of invention, there will be changes in specific embodiments and applications, in conclusion the content of the present specification should
It is interpreted as limitation of the present invention.
Claims (9)
1. a kind of artificial fecundation method of the former carp of crow, which is characterized in that the artificial fecundation method includes：
Parent fish rearing：The 5 former carps of male crow more than age and the 6 former carp of female crow more than age is selected to be put into parent fish rearing pond, parent
It is 1500-2000m that fish, which cultivates pool area,2, depth of water 1.0-1.5m, breeding density 667m2The water surface is no more than 150kg, male and female parent population
The ratio of stocking is 10 ﹕ 1-2, feeds the compound feed for carps of protein content 30-35%, and day feeding volume is the 3-4% of fish body weight,
Sooner or later it respectively feeds once, in the artificial propagation previous moon, feeding volume is kept to original half, and new every 7-10 Tianchongs note
Water 1 time promotes the former carp gonadal maturation of crow；
Parent population selects：Full body surface is selected to have harsh feeling, it is female that abdomen expands in shape, lower abdomen is soft, cloacal aperture is ruddy
Property the former carp of crow；Selection whole body body surface has harsh feeling, there is the hero of milky sperm outflow to scatter into water when slighter compress abdomen
Property the former carp of crow, the male and female combo ratio for selecting the parent population for hastening parturition is 4-5:1；
It hastens parturition：To the selected parent population hastened parturition, disposable injection ocyodinic is carried out, promotes parent population heat, oviposition and production essence, to female
The dosage of parent population injection ocyodinic is 1.0ml/kg, and the dosage to male parent population injection ocyodinic is 0.5ml/kg, wherein, often
The ocyodinic of 1.0ml is by 0.2mg carps pituitary, the salmon gonadotropin releasing hormone analogues of 10 μ g, 3mg maleic acids
Europe ketone and 0.7% physiological saline are configured；
Artificial insemination：Parent population after hastening parturition is put into spawning pond, bath makes oviposition water form flow, and flow Effect time is
16-24h；In parent population heat climax and when starting oviposition, carry out adopting ovum and semen collection, the ripe ovum and essence of acquisition are placed on same
In container, and 50-100ml physiological saline is added in container, gently mix completion insemination, form fertilized eggs；
Unsticking：Diluted yellow mud water is put into washbowl, fertilized eggs are poured into yellow mud water and are gently mixed slowly, make fertilized eggs
It is in close contact with yellow mud water, yellow mud is sticked to fertilized eggs surface and completes unsticking, clear water is used to rinse fertilized eggs after the completion of unsticking；
Hatching：Fertilized eggs are hatched using two sections of hatching methods.
2. the artificial fecundation method of the former carp of crow according to claim 1, which is characterized in that described to female parent population and described
Male parent population injects ocyodinic to be intraperitoneal from 45 ° of injection parent populations of recess inclination on rear side of parent population pectoral fin base portion using syringe,
Needle point is pierced into 1.0cm in parent population body.
3. the artificial fecundation method of the former carp of crow according to claim 1, which is characterized in that the diluted yellow mud water is by 1
The proportion composite of part particulate yellow mud and 7 parts of water stirs.
4. the artificial fecundation method of the former carp of crow according to claim 1, which is characterized in that described to use two sections of hatching methods pair
Fertilized eggs are hatched, including：
Hatch early period, mid-term：Fertilized eggs are put into hatching barrel, using hatching barrel Lotic hatching, flow is controlled, makes fertilized eggs under
Slowly rolling upwards；
Hatch the later stage：Fertilized eggs are moved into tablet incubator and carry out micro- Lotic hatching, are lain on one's side after making fry membrane at the bottom.
5. the artificial fecundation method of the former carp of crow according to claim 4, which is characterized in that the hatching water temperature for 16 DEG C extremely
22 DEG C, the brooding time is 150h to 80h.
6. the artificial fecundation method of the former carp of crow according to claim 4, which is characterized in that the Spawning water is dissolved oxygen amount
More than or equal to the limpid fresh water that 6mg/L, the temperature difference are no more than 2 DEG C, the limpid fresh water meets water quality standard for fishery
(GB11607) regulation.
7. the artificial fecundation method of the former carp of crow according to claim 4, which is characterized in that the fertilization that the hatching barrel is put into
Ovum density is to be put into no more than 50,000 fertilized eggs per 100L water bodys.
8. the artificial fecundation method of the former carp of crow according to claim 4, which is characterized in that the tablet incubator is flat
Pond or Flat bottom container.
9. the artificial fecundation method of the former carp of crow according to claim 4, which is characterized in that the hatching later stage goes out for fry
The period of 1-8h before film.
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