CN107549064A - Cement pit artificial fecundation method in a kind of Scatophagus argus (Linnaeus) room - Google Patents
Cement pit artificial fecundation method in a kind of Scatophagus argus (Linnaeus) room Download PDFInfo
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Abstract
Cement pit artificial fecundation method in a kind of Scatophagus argus (Linnaeus) room, by Scatophagus argus (Linnaeus) parent's reinforced cultivating, parent's sexual gland synchronization modulation, parent to be produced selects, artificial insemination and embryonated egg segmented hatching composition, it is characterized in that late November last year was to 2 months then, 3 ages+Scatophagus argus (Linnaeus) is put into indoor cement pit and carries out reinforced cultivating of surviving the winter, mid-March puts Scatophagus argus (Linnaeus) standby parent's male and female point pond in a suitable place to breed, late November last year to April then, raw salt progressively heat up to 28 29 DEG C of water temperature, salinity 24 28, after being maintained 1 month under above-mentioned water environment conditions, select parent and embryonated egg is obtained by artificial insemination, using embryonated egg segmented hatching pattern, first hatch in hatching cylinder, after be transferred to seed cultivation pool hatching, obtain newly hatched larvae.
Description
Technical field
The present invention relates to money Gyrinocheilidae Scatophagus argus (Linnaeus) category artificial propagation of fish technology, the people of cement pit especially in Scatophagus argus (Linnaeus) room
Work reproduction technique.
Background technology
Scatophagus argus (Linnaeus) (Scatophagus argus) is commonly called as gold drum fish, category Perciformes, Anabantoidei, money Gyrinocheilidae, Scatophagus argus (Linnaeus)
Category.Scatophagus argus (Linnaeus) is eurysaline fishes, is mainly distributed on India-Pacific region, is common in China East Sea, the rivers in South Sea region
Estuary salt-fresh water blending waters.Scatophagus argus (Linnaeus) is the traditional economy fish of coastal areas of southern China pond and cage culture, can be in sea
Cultivated in water, salt-fresh water and fresh water, its seed source is mainly that wild sea is caught, in recent years due to water environment pollution and throughout the year
Caused by sea is caught stock number decline, sea capture Scatophagus argus (Linnaeus) seed can not meet Scatophagus argus (Linnaeus) cultivation market seed supply need
Ask.Meanwhile Scatophagus argus (Linnaeus) is omnivorousness, gregarious phase fish, using algae and small-sized benthonic invertebrates as food, happiness Enteromorpha, water are continuous,
Improvement to water area ecological environment has the function that positive, and the enhancement releasing of appropriate scale is to the Enteromorpha outburst in region and red tide
There is preventive and therapeutic effect Deng environmental pollution.In addition, the Scatophagus argus (Linnaeus) bodily form is graceful, its mottled decorative pattern similar to leopard have view and admire
Value, and a kind of rare ornamental fish.Therefore, a kind of Scatophagus argus (Linnaeus) artificial fecundation method is established, and then realizes stabilization, batch
Seed supply, can effectively solve to expand economic benefit in Scatophagus argus (Linnaeus) industry development, the bottleneck of ecological benefits and social benefit is asked
Topic.
At present, existing artificial propagation techniques mainly have two kinds, first, artificial induction's natural birth under the net cage environment of sea area
Ovum and fertilization, the technology is larger by such environmental effects, and artificial induction's natural spawning and fertilization technique obtain batch embryonated egg
Relative controllability is relatively low；Second, pass through wild type domestication and fortification cultivation and efficient induced spawning agent and artificial induced spawning technology
Realize female and male gametophyte synchronous release.In terms of solving Scatophagus argus (Linnaeus) artificial propagation techniques, lack set of system, complete technical side
Case, and it is that a kind of controllable degree is high to use indoor cement pit to carry out artificial propagation of fish, and the technology hand of large-scale production can be achieved
Section.
The content of the invention
It is an object of the invention to provide cement pit artificial propagation techniques in a kind of Scatophagus argus (Linnaeus) room.
Technical scheme is selected by Scatophagus argus (Linnaeus) parent reinforced cultivating, parent's sexual gland synchronization modulation, parent to be produced, people
Work inseminate and embryonated egg segmented hatching technique step composition, it is characterized in that late November last year to 2 months then, by 3 ages+money
Fish is put into indoor cement pit as standby parent and carries out reinforced cultivating of surviving the winter, and cement cultivation pool area is 18-20m2, the depth of water
1.0m；Cultivation density is 3-6 tails/m2, seawater expanded pellet feed and fresh and alive spiral shell are fed daily, and the upper and lower noon respectively feeds 1 sea
Water expanded pellet feed, 100 ± 10g/ ponds being fed every time, feeding 1 fresh and alive spiral shell afternoon, spiral shell individual particle need to be less than money
Lymphogranuloma inguinale footpath, it is advisable with being adapted to Scatophagus argus (Linnaeus) entirely to eat, spiral shell is scalded 2 times through 70 DEG C of hot water, and normal-temperature water is complete with pin picking after rinsing
Whole spiral shell, complete spiral shell contain muscle and sexual gland, feed 250-300g/ ponds every time, daily soil pick-up 1 time, change water weekly 1 time, change water
Measure as 1/2；March puts Scatophagus argus (Linnaeus) standby parent's male and female point pond in a suitable place to breed, and 3 ages+Scatophagus argus (Linnaeus) male and female are easier to distinguish, and raun body weight is closely male
2 times of fish, raun parent fish rearing pond density are 3-5 tails/m2, a small amount of milter is put into pond, every 60 tail raun is put into 3-5 tails hero
Fish, milter parent fish rearing pond density are 4-6 tails/m2, a small amount of raun is put into pond, every 100 tail milter is put into 3-5 tail rauns,
Daily management method is the same during this, wherein because of the influence of isolating female from male culture and temperature rise factor, raun parent fish rearing pond is ingested
The obvious increase of amount, milter parent fish rearing pond food ration increase unobvious, increased food ration meet that particle is raised with fresh and alive spiral shell
Expect that feeding volume is constant, late November last year in the supreme end of the year, indoor cement pit is survived the winter 20-22 DEG C of initial water temperature, salinity 2-4, and then 1
The moon plays heating, and increasing extent of temperature is 1 DEG C/day, to 24-25 DEG C, 2 months of water temperature, salinity 3-5 is lifted daily, to salinity 18-22, March
Rise and heat up again, increasing extent of temperature is 0.5 DEG C/day, to 27-28 DEG C of water temperature, after stable 10-15 days, lifts salinity 1-3 daily, extremely
Salinity 24-28,28-29 DEG C is warming up to after April money fish parent fish sediment upturning, salinity keeps constant, and during which daily management method is the same,
May can select the stage into parent to be produced；Select belly swell slightly, soft-touch, body surface without raun is hindered, it is fresh to select body colour
Gorgeous, the quick milter of reaction, milter gonopore upward and touched belly and have white seminal fluid to flow out and be in complete drops, milter
Only surveyed sample in parent fish rearing pond, when drawing in the net to check that the milter for having seminal fluid reaches more than 60%, select raun to be produced
It is placed in net cage, cage size is 1.0m × 0.6m × 0.4m, and each net cage places raun 5-8 tails, and 2 gas are placed in net cage
Stone, continuous charge are in wavy, and net cage is placed on parent fish rearing pond equality of temperature with the cement pit of sea brine, covered above the mouth of pond
Shade film lucifuge；Raun is found if checking just before giving birth, and raun is wrapped up with the wet towel wrung out, reduces raun stress reaction, immediately
Prepare ceramic whiteware basin, after 0.9% physiological saline rinse 2 times, 50-150ml 0.9% physiological saline is poured into, in milter parent population
In culture pond, draw in the net to obtain 10-20 tail milters, milter, which is not dehydrated, to be trapped in and draw in the net, and milter is fished for dredger, by milter body surface
Moisture is dried, and gently presses its belly, and the hairbrush being made of pure wool is placed in the interior stirring of physiological saline of ceramic whiteware basin after dipping seminal fluid
It is even, then adopt ovum into ceramic whiteware basin, hairbrush does not stop to stir, after ovum is mined out again semen collection liquid into ceramic whiteware basin, and be sufficiently stirred
It is even, select 3-6 tails milter to provide seminal fluid, after smart ovum is sufficiently mixed, 27-30 DEG C of water temperature, salinity 24- are slowly injected into ceramic whiteware basin
28 seawater filtered through 200 mesh sieve tulles, and do not stop to stir with hairbrush, complete artificial insemination；Incubating oosperm in two stages,
First stage is carried out in cone hatches cylinder, and hatching cylinder diameter is 0.9m, water body 0.17m3, it is each to hatch cylinder hatching embryonated egg
The tails of 10-20 ten thousand, hatching cylinder are placed in water bath, and it is in micro-boiling shape that 1 gas stone continuous charge is placed in cylinder, and the spray that rises reaches cylinder
Wall, water temperature and salinity during artificial insemination are maintained, treat hatching 8 hours, development of fertilized ova to stage gastrul stage, sediments microscope inspection system
Rate of fertilization is counted, rate of fertilization more than 50% is hatched into the ovum in cylinder is transferred in seed cultivation pool and continues to hatch, and first removes during transhipment
Hatch the gas stone in cylinder, water body in cylinder is stood 3-5 minutes, the diameter 20cm made of the soft bolting silk of 130 mesh, deep 10cm band handle
Dredger, upper strata water body gently fishes for ovum back and forth in cylinder, be transferred to immediately after pulling out it is cut-and-dried with hatching equality of temperature filled with salt
The bucket of 10L seawater, it is transported to immediately with hatching cylinder equality of temperature with the seed cultivation pool of sea brine, dredger drags on hatching cylinder upper strata
Take ovum 3-5 times, after water body stands 3-5 minutes again in cylinder, it is bright that ovum quantity is fished on repetition aforesaid operations upper strata when water body is stood
Aobvious to reduce, now 80% embryonated egg has been transferred to seed cultivation pool, hatches remaining ovum in cylinder and treats that water body stands 8-10 again
After minute, long soft-pipe is inserted to hatching cylinder conical bottom, bottom unfertilized egg is drawn using siphonage and discharged, when discharging 2/3 water
After body, flexible pipe, which is placed in cut-and-dried 130 mesh dredgers being shelved on above bucket, collects remaining ovum, and remaining ovum is turned
Seed cultivation pool is transported to, 90% embryonated egg and 10% unfertilized egg in hatching cylinder can be made by above-mentioned transhipment, lock out operation
It is transferred in seed cultivation pool, the full pond brushing dusty blue aquaculture nursery specialized paint of seed cultivation pool, pool wall is smooth, area
For 18-20m2, depth of water 1.0m, gas stone density is evenly arranged in pond as 1.5-2.0/m2, gas stone with a distance from pool side from being not more than
20cm, it is not more than 30cm from pond angular distance, continuous charge is in micro-boiling shape, and the spray scope that rises can be covered to Chi Jiao, seed training
It is 1/4 depth of water to educate pond cloth embryonated egg water level, and cloth embryonated egg is green alga water of the equality of temperature with salt with water, transparency 30-40cm, embryonated egg
Start rupture of membranes after 12 hours in seed cultivation pool.
The present invention outstanding feature be：1) cement pit artificial propagation techniques in Scatophagus argus (Linnaeus) room are solved；2) with existing sea area
Artificial induction compares with natural spawning fertilization technology, the batch production of indoor parent's reinforced cultivating and parent's sexual gland synchronization ripening technique
Degree is high, and the artificial adjustment degree of water body items physicochemical environment index is high, and technological guidance's property is strong；3) with existing genital regulating technology
Compare, indoor cement pit artificial propagation realizes the synchronization of Scatophagus argus (Linnaeus) male and female sexual gland by simulating the technological means of natural propagation environment
Maturation, the use accelerated the ripening with oxytocic drug is farthest reduced, be advantageous to improve hatchability of fertile eggs, it is abnormal to reduce newly hatched larvae
Form quotient, improve seed rearing stage survival rate；4) embryonated egg segmented hatching technique solves the Scatophagus argus (Linnaeus) incubating oosperm cycle
Short, incubation period requires high technical barrier to water environment physical and chemical factor, for money fish fertilized egg feature, reduces during hatching
Loss of the water to embryonated egg is changed, is separated by the transhipment of embryonated egg, is solved incubating oosperm and seed rearing water quality at initial stage is steady
Fixed technical barrier.
Embodiment：
Production technology of the present invention comprises the following steps：
1st, parent's reinforced cultivating：Late November last year is put into interior to 2 months then, using 3 ages+Scatophagus argus (Linnaeus) as standby parent
Reinforced cultivating of surviving the winter is carried out in cement pit, cement cultivation pool area is 18-20m2, depth of water 1.0m；Cultivation density is 3-6 tails/m2,
Seawater expanded pellet feed and fresh and alive spiral shell are fed daily, the upper and lower noon respectively feeds 1 seawater expanded pellet feed, fed every time
100 ± 10g/ ponds, feed 1 fresh and alive spiral shell afternoon, spiral shell individual particle need to be less than Scatophagus argus (Linnaeus) bore, to be adapted to Scatophagus argus (Linnaeus) whole
Eating is advisable, and spiral shell is scalded 2 times through 70 DEG C of hot water, and normal-temperature water use the complete spiral shell of pin picking after rinsing, complete spiral shell containing muscle with
Sexual gland, feeds 250-300g/ ponds every time, daily soil pick-up 1 time, changes water weekly 1 time, quantity of exchanged water 1/2；
2nd, parent's sexual gland synchronization modulation：March puts Scatophagus argus (Linnaeus) standby parent's male and female point pond in a suitable place to breed, and 3 ages+Scatophagus argus (Linnaeus) male and female are relatively held
Easily distinguish, 2 times of the nearly milter of raun body weight, raun parent fish rearing pond density is 3-5 tails/m2, it is put into a small amount of milter in pond, every 60
Tail raun is put into 3-5 tail milters, and milter parent fish rearing pond density is 4-6 tails/m2, a small amount of raun, every 100 tail milter are put into pond
3-5 tail rauns are put into, daily management method is the same during this period, wherein because of the influence of isolating female from male culture and temperature rise factor, it is female
Fish parent fish culture pond food ration substantially increases, and milter parent fish rearing pond food ration increase unobvious, increased food ration is with fresh and alive
Spiral shell meets that pellet feeding volume is constant, late November last year in the supreme end of the year, and indoor cement pit is survived the winter initial water temperature 20-
22 DEG C, salinity 2-4, heated up from January then, increasing extent of temperature is 1 DEG C/day, to 24-25 DEG C, 2 months of water temperature, lifts salinity daily
3-5, to salinity 18-22, being heated up again from March, increasing extent of temperature is 0.5 DEG C/day, to 27-28 DEG C of water temperature, after stable 10-15 days,
Lifting salinity 1-3 daily, to salinity 24-28,28-29 DEG C is warming up to after April money fish parent fish sediment upturning, salinity is kept for constant, the phase
Between daily management method it is the same, May can select the stage into parent to be produced；
3rd, parent to be produced selects：Select belly swell slightly, soft-touch, body surface without raun is hindered, it is bright-coloured, anti-to select body colour
Answer quick milter, by milter gonopore upward and touching belly has white seminal fluid to flow out and be in complete drops, milter only exists
Parent fish rearing is surveyed sample in pond, when drawing in the net to check that the milter for having seminal fluid reaches more than 60%, is selected raun to be produced and is placed in
In net cage, cage size is 1.0m × 0.6m × 0.4m, and each net cage places raun 5-8 tails, 2 gas stones is placed in net cage, even
Continuous inflation be in wavy, and net cage is placed on parent fish rearing pond equality of temperature with the cement pit of sea brine, pond mouth top coverage shading
Film lucifuge；
4th, artificial insemination：Raun is found if checking just before giving birth, raun is wrapped up with the wet towel wrung out, reducing raun stress be anti-
Should, prepare ceramic whiteware basin immediately, after 0.9% physiological saline rinse 2 times, pour into 50-150ml 0.9% physiological saline,
In milter parent fish rearing pond, draw in the net to obtain 10-20 tail milters, milter, which is not dehydrated, to be trapped in and draw in the net, and milter is fished for dredger, will
Milter body surface moisture is dried, and gently presses its belly, and the hairbrush being made of pure wool dips the physiological saline that ceramic whiteware basin is placed in after seminal fluid
Inside stir, then adopt ovum into ceramic whiteware basin, hairbrush does not stop to stir, and semen collection liquid into ceramic whiteware basin, and fills again after ovum is mined out
Divide and stir, select 3-6 tails milter to provide seminal fluid, after smart ovum is sufficiently mixed, be slowly injected into ceramic whiteware basin through 200 mesh sieve thin,tough silk
The seawater (27-30 DEG C of water temperature, salinity 24-28) of net filtration, and do not stop to stir with hairbrush, complete artificial insemination；
5th, embryonated egg segmented is hatched：In two stages, the first stage is carried out incubating oosperm in cone hatches cylinder,
Hatching cylinder diameter is 0.9m, water body 0.17m3, each to hatch the tails of cylinder hatching embryonated egg 10-20 ten thousand, hatching cylinder is placed in water bath,
It is in micro-boiling shape that 1 gas stone continuous charge is placed in cylinder, and the spray that rises reaches casing wall, maintains water temperature and salinity during artificial insemination,
Hatching 8 hours is treated, development of fertilized ova to stage gastrul stage, sediments microscope inspection statistics rate of fertilization, rate of fertilization more than 50% is hatched
Ovum in cylinder, which is transferred in seed cultivation pool, to be continued to hatch, and the gas stone in hatching cylinder is first removed during transhipment, stands water body in cylinder
3-5 minutes, the diameter 20cm made of the soft bolting silk of 130 mesh, deep 10cm band handle dredger, upper strata water body gently drags for back and forth in cylinder
Ovum is taken, the cut-and-dried bucket for filling 10L seawater with salt with hatching equality of temperature is transferred to after pulling out immediately, is transported to and hatches immediately
Cylinder equality of temperature is with the seed cultivation pool of sea brine, and dredger fishes for ovum 3-5 times on hatching cylinder upper strata, and water body stands 3-5 points again in cylinder
Zhong Hou, repetition aforesaid operations upper strata when water body is stood are fished for ovum quantity and significantly reduced, and now 80% embryonated egg has been shifted
To seed cultivation pool, hatch remaining ovum in cylinder and after water body stands 8-10 minutes again, long soft-pipe is inserted to hatching cylinder circular cone
Bottom, bottom unfertilized egg is drawn using siphonage and discharged, after 2/3 water body is discharged, flexible pipe is placed in cut-and-dried be shelved on
Collect remaining ovum in 130 mesh dredgers above bucket, and by remaining ovum transport to seed cultivation pool, by above-mentioned transhipment,
Lock out operation can be such that 90% embryonated egg and 10% unfertilized egg in hatching cylinder is transferred in seed cultivation pool, seed cultivation pool
Full pond brushing dusty blue aquaculture nursery specialized paint, pool wall is smooth, area 18-20m2, depth of water 1.0m, uniform cloth in pond
Gas stone density is put as 1.5-2.0/m2, gas stone is not more than 30cm from 20cm is not more than with a distance from pool side, from pond angular distance, continuously fills
Gas is in micro-boiling shape, and the spray scope that rises can be covered to Chi Jiao, and seed cultivation pool cloth embryonated egg water level is 1/4 depth of water, and cloth is fertilized
Ovum is green alga water of the equality of temperature with salt with water, transparency 30-40cm, and embryonated egg starts rupture of membranes in seed cultivation pool after 12 hours.
By the method, Shanghai Aquatic Product Institute Fengxian scientific base was in the 5-6 months in 2017, using 3 ages+Scatophagus argus (Linnaeus) parent
This, through artificial insemination, obtains 920,000, embryonated egg, rate of fertilization 65.7%；Segmented hatching, which obtains, in embryonated egg room just incubates son
The tail of fish 78.0 ten thousand, incubation rate 84.8%.
Claims (1)
1. cement pit artificial fecundation method in a kind of Scatophagus argus (Linnaeus) room, by Scatophagus argus (Linnaeus) parent reinforced cultivating, parent's sexual gland synchronization modulation,
Parent to be produced selects, artificial insemination and embryonated egg segmented hatching technique step form, it is characterized in that late November last year extremely works as
In 2 months years, 3 ages+Scatophagus argus (Linnaeus) is put into indoor cement pit as standby parent and carries out reinforced cultivating of surviving the winter, cement cultivation pool area
For 18-20m2, depth of water 1.0m；Cultivation density is 3-6 tails/m2, seawater expanded pellet feed and fresh and alive spiral shell are fed daily, it is upper and lower
Noon respectively feeds 1 seawater expanded pellet feed, feeds 100 ± 10g/ ponds every time, feeds 1 fresh and alive spiral shell afternoon, and spiral shell is single
Particle need to be less than Scatophagus argus (Linnaeus) bore, be advisable with being adapted to Scatophagus argus (Linnaeus) entirely to eat, and spiral shell is scalded 2 times through 70 DEG C of hot water, normal-temperature water punching
The complete spiral shell of pin picking is used after washing, complete spiral shell contains muscle and sexual gland, feeds 250-300g/ ponds every time, daily soil pick-up 1 time, often
Zhou Huanshui 1 time, quantity of exchanged water 1/2；March puts Scatophagus argus (Linnaeus) standby parent's male and female point pond in a suitable place to breed, and 3 ages+Scatophagus argus (Linnaeus) male and female are easier to area
Point, 2 times of the nearly milter of raun body weight, raun parent fish rearing pond density is 3-5 tails/m2, a small amount of milter is put into pond, and every 60 tail is female
Fish is put into 3-5 tail milters, and milter parent fish rearing pond density is 4-6 tails/m2, a small amount of raun is put into pond, and every 100 tail milter is put into
3-5 tail rauns, daily management method is the same during this period, wherein because of the influence of isolating female from male culture and temperature rise factor, raun parent
Fish culture pond food ration substantially increases, and milter parent fish rearing pond food ration increase unobvious, increased food ration is with fresh and alive spiral shell
To meet, pellet feeding volume is constant, late November last year in the supreme end of the year, and indoor cement pit is survived the winter 20-22 DEG C of initial water temperature,
Salinity 2-4, heated up from January then, increasing extent of temperature is 1 DEG C/day, to 24-25 DEG C, 2 months of water temperature, lifts salinity 3-5 daily, extremely
Salinity 18-22, heats up again from March, and increasing extent of temperature is 0.5 DEG C/day, to 27-28 DEG C of water temperature, after stable 10-15 days, carries daily
Salinity 1-3 is risen, to salinity 24-28,28-29 DEG C is warming up to after April money fish parent fish sediment upturning, salinity keeps constant, during which daily
Management method is the same, and May can select the stage into parent to be produced；Select belly swell slightly, soft-touch, body surface without hinder it is female
Fish, select bright-coloured body colour, the quick milter of reaction, by milter gonopore upward and touching belly has white seminal fluid to flow out and in complete
Drops, milter only surveys sample in parent fish rearing pond, when drawing in the net to check that the milter for having seminal fluid reaches more than 60%, chooses
Raun to be produced is selected to be placed in net cage, cage size is 1.0m × 0.6m × 0.4m, and each net cage places raun 5-8 tails, net cage
2 gas stones of middle placement, continuous charge is in wavy, and net cage is placed on parent fish rearing pond equality of temperature with the cement pit of sea brine,
Coverage shading film lucifuge above the mouth of pond；Raun just before giving birth, by raun is wrapped up with the wet towel wrung out, and reduces raun stress reaction, with
Prepare ceramic whiteware basin, after 0.9% physiological saline rinse 2 times, pour into 50-150ml 0.9% physiological saline, in milter parent
In fish culture pond, draw in the net to obtain 10-20 tail milters, milter, which is not dehydrated, to be trapped in and draw in the net, and milter is fished for dredger, by milter body
Table moisture is dried, and gently presses its belly, and the hairbrush being made of pure wool is placed in stirring in the physiological saline of ceramic whiteware basin after dipping seminal fluid
Uniformly, ovum is then adopted into ceramic whiteware basin, and hairbrush does not stop to stir, and semen collection liquid into ceramic whiteware basin, and is sufficiently stirred again after ovum is mined out
Uniformly, select 3-6 tails milter to provide seminal fluid, after smart ovum is sufficiently mixed, 27-30 DEG C of water temperature, salinity are slowly injected into ceramic whiteware basin
The seawater that 24-28 filters through 200 mesh sieve tulles, and do not stop to stir with hairbrush, complete artificial insemination；Incubating oosperm is divided to two ranks
Section, first stage are carried out in cone hatches cylinder, and hatching cylinder diameter is 0.9m, water body 0.17m3, it is each hatch cylinder hatching by
The smart tails of ovum 10-20 ten thousand, hatching cylinder are placed in water bath, and it is in micro-boiling shape that 1 gas stone continuous charge is placed in cylinder, and the spray that rises reaches
Casing wall, water temperature and salinity during artificial insemination are maintained, treat hatching 8 hours, development of fertilized ova to stage gastrul stage, sediments microscope inspection
Rate of fertilization is counted, rate of fertilization more than 50% is hatched into the ovum in cylinder is transferred in seed cultivation pool and continues to hatch, and first moves during transhipment
Go out the gas stone hatched in cylinder, water body in cylinder is stood 3-5 minutes, diameter 20cm, deep 10cm band made of the soft bolting silk of 130 mesh
Handle dredger, upper strata water body gently fishes for ovum back and forth in cylinder, is transferred to immediately after pulling out cut-and-dried with hatching equality of temperature with salt Sheng
There is the bucket of 10L seawater, be transported to immediately with hatching cylinder equality of temperature with the seed cultivation pool of sea brine, dredger is on hatching cylinder upper strata
Fish for ovum 3-5 times, after water body stands 3-5 minutes again in cylinder, repeat aforesaid operations until ovum quantity is fished on upper strata when water body is stood
Significantly reduce, now 80% embryonated egg has been transferred to seed cultivation pool, hatches remaining ovum in cylinder and treats that water body stands 8- again
After 10 minutes, long soft-pipe is inserted to hatching cylinder conical bottom, bottom unfertilized egg is drawn using siphonage and discharged, when discharge 2/3
After water body, flexible pipe, which is placed in cut-and-dried 130 mesh dredgers being shelved on above bucket, collects remaining ovum, and by remaining ovum
Be transported to seed cultivation pool, by above-mentioned transhipment, lock out operation can make 90% embryonated egg in hatching cylinder and 10% it is unfertilized
Ovum is transferred in seed cultivation pool, and the full pond brushing dusty blue aquaculture nursery specialized paint of seed cultivation pool, pool wall is smooth, face
Product is 18-20m2, depth of water 1.0m, gas stone density is evenly arranged in pond as 1.5-2.0/m2, gas stone with a distance from pool side from being not more than
20cm, it is not more than 30cm from pond angular distance, continuous charge is in micro-boiling shape, and the spray scope that rises can be covered to Chi Jiao, seed training
It is 1/4 depth of water to educate pond cloth embryonated egg water level, and cloth embryonated egg is green alga water of the equality of temperature with salt with water, transparency 30-40cm, embryonated egg
Start rupture of membranes after 12 hours in seed cultivation pool.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108184727A (en) * | 2018-03-22 | 2018-06-22 | 广西壮族自治区水产科学研究院 | A kind of artificial fecundation method of the former carp of crow |
| CN108967278A (en) * | 2018-08-10 | 2018-12-11 | 深圳大学 | A kind of artificial fecundation method of Scatophagus argus (Linnaeus) |
| CN113508763A (en) * | 2021-04-21 | 2021-10-19 | 安徽农业大学 | Indoor breeding method of Taishi goldfish |
| CN113615608A (en) * | 2021-08-31 | 2021-11-09 | 陵水晨海种业有限公司 | Artificial breeding method of salangid |
| CN113661946A (en) * | 2021-07-30 | 2021-11-19 | 陵水晨海种业有限公司 | Outdoor pond culture method for silver drum fish |
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| CN102742530A (en) * | 2012-07-20 | 2012-10-24 | 中国科学院南海海洋研究所 | Method for nature spawning and fertilization of scatophagus argus by artificial induction |
| CN105557601A (en) * | 2016-03-14 | 2016-05-11 | 上海市水产研究所 | Scatophagus argus and macrobrachium rosenbergii intercropping overwintering mode |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102742530A (en) * | 2012-07-20 | 2012-10-24 | 中国科学院南海海洋研究所 | Method for nature spawning and fertilization of scatophagus argus by artificial induction |
| CN105557601A (en) * | 2016-03-14 | 2016-05-11 | 上海市水产研究所 | Scatophagus argus and macrobrachium rosenbergii intercropping overwintering mode |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108184727A (en) * | 2018-03-22 | 2018-06-22 | 广西壮族自治区水产科学研究院 | A kind of artificial fecundation method of the former carp of crow |
| CN108967278A (en) * | 2018-08-10 | 2018-12-11 | 深圳大学 | A kind of artificial fecundation method of Scatophagus argus (Linnaeus) |
| CN113508763A (en) * | 2021-04-21 | 2021-10-19 | 安徽农业大学 | Indoor breeding method of Taishi goldfish |
| CN113661946A (en) * | 2021-07-30 | 2021-11-19 | 陵水晨海种业有限公司 | Outdoor pond culture method for silver drum fish |
| CN113661946B (en) * | 2021-07-30 | 2022-07-12 | 陵水晨海种业有限公司 | Outdoor pond culture method for silver drum fish |
| CN113615608A (en) * | 2021-08-31 | 2021-11-09 | 陵水晨海种业有限公司 | Artificial breeding method of salangid |
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