CN104221952A - Artificial reproduction method of chalcalburnus chalcoides aralensis - Google Patents
Artificial reproduction method of chalcalburnus chalcoides aralensis Download PDFInfo
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- CN104221952A CN104221952A CN201310728350.5A CN201310728350A CN104221952A CN 104221952 A CN104221952 A CN 104221952A CN 201310728350 A CN201310728350 A CN 201310728350A CN 104221952 A CN104221952 A CN 104221952A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention discloses an artificial reproduction method of chalcalburnus chalcoides aralensis. The artificial reproduction method includes steps of rearing and selecting parent fishes, artificially spawning, inseminating and incubating. The fully-artificial reproduction method which is reasonable, high in operability and massive in production of fry of chalcalburnus chalcoides aralensis is provided, and by optimizing the parent-fish rearing technology and utilizing three different spawning medicines for artificial spawning and inseminating, spawning rate, roe fertility rate and fry incubation rate in parent fish reproduction are increased to 88.2%, 97.0% and 83.64% respectively. The artificial reproduction method is scientific, reasonable and high in operability, industrialized production can be realized by the artificial reproduction method, fry can be provided for large-scale breeding, and high economic benefits are increased.
Description
Technical field
The present invention relates to artificial freshwater-fish breeding method, particularly a kind of Chalcalbumus chalcoides aralensis artificial fecundation method.
Background technology
Chalcalbumus chalcoides aralensis (Chalcalburnus Chalcoides aralensis) is subordinate to Cyprinidae, refined sieve subfamily, Chalcalbumus chalcoides aralensis genus.This fishing gear has the merits such as strong stress resistance, feeding habits are wide, meat is good, is the famous and precious Species of Rare Fish from Qingdao of Central Asia.Chalcalbumus chalcoides aralensis was wild species originally, and calendar year 2001 introduces China first by one of applicant.
At present, the research of breeding biology and aquaculture organism aspect of Chalcalbumus chalcoides aralensis has been reported.The introduction of wild fish, domestication and breeding and pond cultivation technology successfully, and achieve effect at China's saline-alkali water and freshwater cultivation.But also not forming the technical system of a set of maturation in artificial propagation, still there are problems in parent fish rearing, induced spawning method, incubating oosperm aspect.Because this fish individuality is little; fish brood amount is low; the individual gonad development of parent population differs greatly; how ovary development accelerating is synchronous; guarantee is laid eggs parent population quantity and concentrated egg laying amount; improving the overall fertilization rate of whole artificial propagation fish, incubation rate and survival rate, is the key point realizing the large-scale production of Chalcalbumus chalcoides aralensis seed.
Summary of the invention
The present invention is directed to the problems referred to above and research and develop a kind of Chalcalbumus chalcoides aralensis artificial fecundation method; its object is to: provide that a kind of method is reasonable, workable, the whole artificial propagation method of the Chalcalbumus chalcoides aralensis seed that is produced on a large scale; by optimizing parent fish rearing technology; use three kinds of different oxytocic drugs to carry out artificial induced spawning, artificial insemination, improve the spawning rate of breeding parent population, fish-egg fertilization rate and fry hatching rate.
Technical solution of the present invention:
A kind of Chalcalbumus chalcoides aralensis artificial fecundation method, is characterized in that: described a kind of Chalcalbumus chalcoides aralensis artificial fecundation method is a kind of Chalcalbumus chalcoides aralensis artificial induced spawning natural insemination propagation method, comprises the following steps:
1, parent fish rearing and selecting: produce and incubate front 30-40 days, select 3 age in winter parent population, the milter body weight selected is 45-60g, and raun body weight is 60-100g, and divides pond reinforced cultivating by female milter, and feed is dregs of beans, day feeding volume be the 5-8% of body weight;
2, artificial induced spawning: constant about 24 DEG C in water temperature, select parent population and hasten parturition from storage pond, holding pond, injection Leuteinizing hormone releasing hormone analogue (LRH-A
2), maleic acid DOM (DOM) and human chorionic gonadtropin (HCG), three's hybrid injection, Leuteinizing hormone releasing hormone analogue (LRH-A
2), maleic acid DOM (DOM) and human chorionic gonadtropin (HCG) injected dose be: LRH-A
2, 3-5 μ g/KG, DOM, 2mg/KG, HCG, 400-600UI/KG, adopt pectoral fin lumbar injection, milter injected dose reduces by half;
3, Process of Insemination: adopt artificial induced spawning naturally to inseminate, parent population after hastening parturition is put into net cage of laying eggs, case of laying eggs uses 100 mesh sieve silks to make and forms, specification is long 2.5m × wide 1.0m × high 1.0m, case of laying eggs is placed in indoor water mud sump, cement pit water body is long 5m × wide 8m × high 1.3m, 100 groups of fishes thrown in by every net cage, lay eggs and oxygenation with water pump circulation stimulation by running water fish, heater cycle water is utilized to debug water 23-25 DEG C, after hastening parturition during drug effect time 14-18, inspection is laid eggs case, when finding that water body has a large amount of fertilized egg, parent population is shifted out, collect fertilized egg,
4, cylinder hatching pail hatching: fertilized egg is after clear water rinses, pour cylinder hatching pail into, every barrel put 50,000 ovum about, hatching pail diameter 15cm, high 40cm, be placed in the incubation tank filling clear water, use the micro-Lotic hatching of filter backpulsing, incubation tank water is changed 2-3 time every day, when hatching pail 50% fry membrane, is poured on by fish embryo in bucket in mounted planar slot mesh sheet, hatching water temperature keeps 23-25 DEG C, fish embryo 58-72h membrane.
A kind of Chalcalbumus chalcoides aralensis artificial fecundation method, is characterized in that: described a kind of Chalcalbumus chalcoides aralensis artificial fecundation method is a kind of Chalcalbumus chalcoides aralensis artificial induced spawning intervention fertilization propagation method, comprises the steps:
1, parent fish rearing and selecting: produce and incubate front 30-40 days, select 3 age in winter parent population, the milter body weight selected is 45-60g, and raun body weight is 60-100g, and divides pond reinforced cultivating by female milter, and feed is dregs of beans, day feeding volume be the 5-8% of body weight;
2, artificial induced spawning: constant about 24 DEG C in water temperature, select parent population and hasten parturition from storage pond, holding pond, injection Leuteinizing hormone releasing hormone analogue (LRH-A
2), maleic acid DOM (DOM) and human chorionic gonadtropin (HCG), three's hybrid injection, Leuteinizing hormone releasing hormone analogue (LRH-A
2), maleic acid DOM (DOM) and human chorionic gonadtropin (HCG) injected dose be: LRH-A
2, 3-5 μ g/KG, DOM, 2mg/KG, HCG, 400-600UI/KG, adopt pectoral fin lumbar injection, milter injected dose reduces by half;
3, Process of Insemination: adopt manual intervention insemination, being divided by the female milter of hastening parturition is placed in different egg case, lay eggs and oxygenation with water pump circulation stimulation by running water fish, heater is utilized to debug water 23-25 DEG C, after parent population is hastened parturition, during drug effect time 14-18h, raun is checked every 1h, extrude raun belly gently, gonopore has a small amount of ovum grain to flow out, and carries out intervention fertilization, the female fish-egg of every 3 tail and 1 tail milter seminal fluid are inseminated, fertilized egg is slightly washed with clear water, stirs 30min with feather, pours taper hatching pail into;
4, centrum hatching pail hatching: after fertilized egg being washed with clear water, stir 30min with feather, put it into hatching pail after ovum mucus comes off, centrum hatching pail volume 109.4L(diameter external diameter 95.0cm/ internal diameter 66cm, high 96.0cm), about every barreled 500,000 ovum, use micro-Lotic hatching, fish embryonic development 60h, when fry hatches 1/3 quantity, prelarva will be hatched and constantly shift out hatching pail, and pour circular groove into and carry out fry rearing, hatching water temperature keeps 23-25 DEG C, fish embryo 58-72h membrane.
Beneficial effect of the present invention:
The invention provides a kind of method reasonable, workable, be produced on a large scale the whole artificial propagation method of Chalcalbumus chalcoides aralensis seed, by optimizing parent fish rearing technology, three kinds of different oxytocic drugs are used to carry out artificial induced spawning, artificial insemination, improve the spawning rate of breeding parent population, fish-egg fertilization rate and fry hatching rate, parent population spawning rate brings up to 88.2 %, fish-egg fertilization rate brings up to 97.0%, fry hatching rate is up to 83.64%, this method is scientific and reasonable, workable, this technology is utilized to realize factorial praluction, for large-scale farming provides seed, obtain good economic benefit.
Embodiment
Below in conjunction with embodiment, the present invention is described further.
Embodiment:
1, parent fish rearing and selecting
Product incubates first 30 days, select 3 age in winter parent population, and divide pond reinforced cultivating by female milter, feed is dregs of beans, day feeding volume be the 5-8% of body weight, ripe raun 100 tail of selectivity, belly is full, soft, and gonopore mastoid process is large and give prominence to, and hole circle becomes blush, total length 19.22-23.71cm, body weight 55.96-97.46 g, ripe milter 120 tail of selectivity, total length 17.89-20.61cm, body weight 45.11-65.60g, health is slender, and healthy scale is complete, and head gill cover place hand is touched coarsely has star-pursuing, extruding gonopore gently has seminal fluid to flow out, and seminal fluid chance water scatters rapidly.
2, artificial induced spawning
When the constant 23-25 DEG C of water temperature, from storage pond, holding pond, select parent population (female-male proportion is 1:1.2) hasten parturition, injectable drug is Leuteinizing hormone releasing hormone analogue (LRH-A2), maleic acid DOM (DOM) and the human chorionic gonadtropin (HCG) that Ningbo bio-pharmaceuticals three factory produces, three's hybrid injection, injected dose is: LRH-A
23-5 μ g/KG, DOM 2mg/KG, HCG 400-600UI/KG.Adopt pectoral fin lumbar injection, milter injected dose reduces by half.
3, fertilization process
Natural spawning: parent population of hastening parturition puts into net cage of laying eggs, lays eggs and oxygenation with water pump circulation stimulation by running water fish, and utilize heater cycle water to debug water temperature 23-25 DEG C, drug effect time 14-18h, after the 18h that hastens parturition, shifts out parent population, collects fertilized egg.
Artificial insemination: at drug effect time 14-18h, checks raun gonopore every 1h and extrudes belly gently, finds that there is ovum grain and flows out, immediate intervention is fertilized, fertilized egg is rinsed with clear water and feather stirs 30min, pours centrum hatching pail (putting 500,000 ovum for every barrel) into, micro-Lotic hatching.
4, incubating oosperm process
Fertilized egg, after clear water rinses, pours cylinder hatching pail into.Every barrel put 50,000 ovum about, hatching pail is placed in the incubation tank filling clear water, use the micro-Lotic hatching of filter backpulsing, incubation tank water is changed 2-3 time every day, when hatching pail 50% fry membrane, be poured on by fish embryo in bucket in mounted planar slot mesh sheet, hatching water temperature keeps 23-25 DEG C, the about 58-72h membrane of fish embryonic development.
Table 1 is gained testing result of the present invention
Claims (2)
1. a Chalcalbumus chalcoides aralensis artificial fecundation method, is characterized in that: described a kind of Chalcalbumus chalcoides aralensis artificial fecundation method is a kind of Chalcalbumus chalcoides aralensis artificial induced spawning natural insemination propagation method, comprises the following steps:
1), parent fish rearing and selecting: produce and incubate front 30-40 days, select 3 age in winter parent population, the milter body weight selected is 45-60g, and raun body weight is 60-100g, and divides pond reinforced cultivating by female milter, and feed is dregs of beans, day feeding volume be the 5-8% of body weight;
2), artificial induced spawning: constant about 24 DEG C in water temperature, select parent population and hasten parturition from storage pond, holding pond, injection Leuteinizing hormone releasing hormone analogue (LRH-A
2), maleic acid DOM (DOM) and human chorionic gonadtropin (HCG), three's hybrid injection, Leuteinizing hormone releasing hormone analogue (LRH-A
2), maleic acid DOM (DOM) and human chorionic gonadtropin (HCG) injected dose be: LRH-A
2, 3-5 μ g/KG, DOM, 2mg/KG, HCG, 400-600UI/KG, adopt pectoral fin lumbar injection, milter injected dose reduces by half;
3), Process of Insemination: adopt artificial induced spawning naturally to inseminate, parent population after hastening parturition is put into net cage of laying eggs, case of laying eggs uses 100 mesh sieve silks to make and forms, specification is long 2.5m × wide 1.0m × high 1.0m, case of laying eggs is placed in indoor water mud sump, cement pit water body is long 5m × wide 8m × high 1.3m, 100 groups of fishes thrown in by every net cage, lay eggs and oxygenation with water pump circulation stimulation by running water fish, heater cycle water is utilized to debug water 23-25 DEG C, after hastening parturition during drug effect time 14-18, inspection is laid eggs case, when finding that water body has a large amount of fertilized egg, parent population is shifted out, collect fertilized egg,
4), cylinder hatching pail hatching: fertilized egg is after clear water rinses, pour cylinder hatching pail into, every barrel put 50,000 ovum about, hatching pail diameter 15cm, high 40cm, be placed in the incubation tank filling clear water, use the micro-Lotic hatching of filter backpulsing, incubation tank water is changed 2-3 time every day, when hatching pail 50% fry membrane, is poured on by fish embryo in bucket in mounted planar slot mesh sheet, hatching water temperature keeps 23-25 DEG C, fish embryo 58-72h membrane.
2. a Chalcalbumus chalcoides aralensis artificial fecundation method, is characterized in that: described a kind of Chalcalbumus chalcoides aralensis artificial fecundation method is a kind of Chalcalbumus chalcoides aralensis artificial induced spawning intervention fertilization propagation method, comprises the steps:
1), parent fish rearing and selecting: produce and incubate front 30-40 days, select 3 age in winter parent population, the milter body weight selected is 45-60g, and raun body weight is 60-100g, and divides pond reinforced cultivating by female milter, and feed is dregs of beans, day feeding volume be the 5-8% of body weight;
2), artificial induced spawning: constant about 24 DEG C in water temperature, select parent population and hasten parturition from storage pond, holding pond, injection Leuteinizing hormone releasing hormone analogue (LRH-A
2), maleic acid DOM (DOM) and human chorionic gonadtropin (HCG), three's hybrid injection, Leuteinizing hormone releasing hormone analogue (LRH-A
2), maleic acid DOM (DOM) and human chorionic gonadtropin (HCG) injected dose be: LRH-A
2, 3-5 μ g/KG, DOM, 2mg/KG, HCG, 400-600UI/KG, adopt pectoral fin lumbar injection, milter injected dose reduces by half;
3), Process of Insemination: adopt manual intervention insemination, being divided by the female milter of hastening parturition is placed in different egg case, lay eggs and oxygenation with water pump circulation stimulation by running water fish, heater is utilized to debug water 23-25 DEG C, after parent population is hastened parturition, during drug effect time 14-18h, raun is checked every 1h, extrude raun belly gently, gonopore has a small amount of ovum grain to flow out, and carries out intervention fertilization, the female fish-egg of every 3 tail and 1 tail milter seminal fluid are inseminated, fertilized egg is slightly washed with clear water, stirs 30min with feather, pours taper hatching pail into;
4), centrum hatching pail hatching: after fertilized egg being washed with clear water, stir 30min with feather, put it into hatching pail after ovum mucus comes off, centrum hatching pail volume 109.4L(diameter external diameter 95.0cm/ internal diameter 66cm, high 96.0cm), about every barreled 500,000 ovum, use micro-Lotic hatching, fish embryonic development 60h, when fry hatches 1/3 quantity, prelarva will be hatched and constantly shift out hatching pail, and pour circular groove into and carry out fry rearing, hatching water temperature keeps 23-25 DEG C, fish embryo 58-72h membrane.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105409855A (en) * | 2015-12-16 | 2016-03-23 | 宜章洪成特种水产繁育场 | Fry culturing method |
| CN106172106A (en) * | 2016-07-08 | 2016-12-07 | 天津现代晨辉科技集团有限公司 | A kind of Carassius auratus artificial incubation method |
| CN107333680A (en) * | 2016-04-29 | 2017-11-10 | 岚皋县绿水生态农业有限责任公司 | A kind of artificial breeding method of more squama shovel jaw fishes and special hatchery |
| CN108739542A (en) * | 2018-06-15 | 2018-11-06 | 中国水产科学研究院黑龙江水产研究所 | Three pieces of fish artificial fecundation methods of pearl star |
| CN110896892A (en) * | 2019-12-17 | 2020-03-24 | 昆明市水产科学研究所 | Artificial breeding method for silver whitefish |
| CN113287548A (en) * | 2021-07-05 | 2021-08-24 | 镇康县水产技术推广站 | Artificial propagation method for yellow-shelled fishes |
| CN113545306A (en) * | 2021-07-14 | 2021-10-26 | 安徽冠禅生物科技有限公司 | Breeding method of freshwater fish |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1518864A (en) * | 2003-09-02 | 2004-08-11 | 天津市换新水产良种场 | Method of distant hybridization between subfamilies of cyprinid fish |
| CN101438686A (en) * | 2008-12-30 | 2009-05-27 | 张晓光 | Induced spawning and breeding method for Erythroculter ilishaeformis |
| CN102246709A (en) * | 2010-05-20 | 2011-11-23 | 鸡西锅盔山庄有限公司 | Method for artificially cultivating and breeding Khanka Erythroculter ilishaeformis |
-
2013
- 2013-12-26 CN CN201310728350.5A patent/CN104221952B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1518864A (en) * | 2003-09-02 | 2004-08-11 | 天津市换新水产良种场 | Method of distant hybridization between subfamilies of cyprinid fish |
| CN101438686A (en) * | 2008-12-30 | 2009-05-27 | 张晓光 | Induced spawning and breeding method for Erythroculter ilishaeformis |
| CN102246709A (en) * | 2010-05-20 | 2011-11-23 | 鸡西锅盔山庄有限公司 | Method for artificially cultivating and breeding Khanka Erythroculter ilishaeformis |
Non-Patent Citations (8)
| Title |
|---|
| 吴维新等: ""翘嘴鲌苗种批量化生产技术（Ⅰ）翘嘴鲌人工催产和产卵"", 《内陆水产》, 25 December 2004 (2004-12-25) * |
| 宓国强等: ""翘嘴红鲌人工繁殖技术研究"", 《浙江海洋学院学报(自然科学版)》, 30 March 2004 (2004-03-30) * |
| 张建铭等: ""黑尾近红鲌人工繁殖技术研究"", 《水产科技情报》, 1 April 2011 (2011-04-01) * |
| 徐丽娟: ""翘嘴红鲌人工繁殖技术"", 《河北渔业》, 20 March 2010 (2010-03-20) * |
| 王信海等: ""盐度对咸海卡拉白鱼生长及组织学特征的影响"", 《中国水产科学》, 15 September 2008 (2008-09-15) * |
| 王宝云等: ""星云白鱼人工驯养繁殖技术研究"", 《云南农业》, 5 October 2010 (2010-10-05) * |
| 王芳: ""翘嘴鲌人工催产及孵化技术"", 《河南水产》, 15 February 2010 (2010-02-15) * |
| 蔺玉华等: ""池塘驯养咸海卡拉白鱼某些生物学特性研究"", 《天津师范大学学报(自然科学版)》, 30 December 2004 (2004-12-30) * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105409855A (en) * | 2015-12-16 | 2016-03-23 | 宜章洪成特种水产繁育场 | Fry culturing method |
| CN107333680A (en) * | 2016-04-29 | 2017-11-10 | 岚皋县绿水生态农业有限责任公司 | A kind of artificial breeding method of more squama shovel jaw fishes and special hatchery |
| CN106172106A (en) * | 2016-07-08 | 2016-12-07 | 天津现代晨辉科技集团有限公司 | A kind of Carassius auratus artificial incubation method |
| CN108739542A (en) * | 2018-06-15 | 2018-11-06 | 中国水产科学研究院黑龙江水产研究所 | Three pieces of fish artificial fecundation methods of pearl star |
| CN110896892A (en) * | 2019-12-17 | 2020-03-24 | 昆明市水产科学研究所 | Artificial breeding method for silver whitefish |
| CN113287548A (en) * | 2021-07-05 | 2021-08-24 | 镇康县水产技术推广站 | Artificial propagation method for yellow-shelled fishes |
| CN113287548B (en) * | 2021-07-05 | 2022-11-25 | 镇康县水产技术推广站 | Artificial propagation method for yellow-shelled fishes |
| CN113545306A (en) * | 2021-07-14 | 2021-10-26 | 安徽冠禅生物科技有限公司 | Breeding method of freshwater fish |
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