CN1518864A - Method of distant hybridization between subfamilies of cyprinid fish - Google Patents
Method of distant hybridization between subfamilies of cyprinid fish Download PDFInfo
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- CN1518864A CN1518864A CNA03144234XA CN03144234A CN1518864A CN 1518864 A CN1518864 A CN 1518864A CN A03144234X A CNA03144234X A CN A03144234XA CN 03144234 A CN03144234 A CN 03144234A CN 1518864 A CN1518864 A CN 1518864A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
A distant hydridization method between the subfamilies of cyprinid includes chealth eugamic male and female parents catalyzing female, artificial fertilization, incubation and naturalizing to obtain to descendant with strong bionergy. Its advantages are high disease resistance, high survival rate and high growth speed.
Description
Technical field
The invention belongs to fishery and culture the field, relate in a kind of Cyprinidae distant crossing method between three subfamily fish.
Background technology
Known cyprinid fish subfamily intermolecular hybrid, owing to can not find suitable distant hybridization material, most combinations can not be hybridized success; Industry was once done 112 hybrid combination tests of 32 kinds of fish of 3 orders, 5 sections, between wherein planting, intergeneric cross, most hybridization can obtain vital offspring, but the hybridization of two kinds of fish all can not obtain vital diplontic crossbreed in batches between subfamily except that flathead ♀ * triangular bream ♂, flathead ♀ * megalobrama amblycephala ♂ combination.Fish genetic is formed difference and is caused hybridizing infertile or sterile between subfamily, can't obtain vital hybrid generation or because of hybrid dysgenesis, can't go down to posterity.(the Chevassus of breeding man is arranged, nineteen eighty-three) by the hybrid genetic material being formed the analysis of (genetic recombination), to the growth trend of " xenogenesis insemination " the smart ovum combination in back, proposed smart ovum in conjunction with the diplontic theory of formation hybrid, but existing engineering practice hybrid there is not vitality mostly.Thereby industry is demanded a kind of cyprinid fish inter-subfamily distant hybridization technology appearance with high fertilization rate, incubation rate and weed survival rate urgently.
Summary of the invention
Problem to be solved by this invention is to overcome the above-mentioned defective that aforementioned techniques exists, and a kind of method of cyprinid fish inter-subfamily distant hybridization is provided.
The present invention solves its technical problem and takes following technical scheme to realize, method according to a kind of cyprinid fish inter-subfamily distant hybridization provided by the invention, comprise and select healthy, anosis, sexually matured male parent fish or maternal fish, then maternal fish is carried out catalysis, adopt the manual method insemination, the unsticking hatching, to the juvenile fish bait acclimatization culture that hatches, wherein, maternal fish is selected Cyprinus carpio L. Mirror for use, and the male parent fish is selected blunt-snout gurnards for use.
The present invention solves its technical problem and can also take following technical scheme further to realize:
Aforementioned cross method wherein, carries out artificial catalysis to maternal fish Cyprinus carpio L. Mirror, with every kg fish body weight with 6.0 μ g luteotropin releasing hormone d-ala analog (LHRH-A
2) hasten parturition with 2.0mg maleic acid DOM (DOM) and 1000 international unit human chorionic gonadtropin mixing ocyodinics.
Aforementioned cross method behind the hybridization parent population artificial induced spawning, arrives effect and carries out artificial insemination during the time immediately, and wherein, artificial insemination adopts 1 tail raun to join 2 tail milters.
Aforementioned cross method, wherein, the hatching of described unsticking is when artificial insemination, after smart feritilization of ovum 3-5 minute, fertilized egg is sticked to be positioned in advance under the depth of water 8-10cm on the ovum net sheet;
After the artificial insemination, with the net sheet of ovum hang in net cage, and make about dried up 10cm of a net pitch of fins, net cage is arranged in the fishpond of about 2 meters of the depth of waters, and about the spacing 80cm between each net cage, water temperature is arranged on about 20 ℃, time is 5 days, and the fertilized egg on the net sheet is hatched fry.
Aforementioned cross method, described juvenile fish bait acclimatization culture to hatching, wherein, to the fry that the hatches ripe egg yolk slurry of throwing something and feeding, during be 3-5 days, ripe egg yolk input ratio is that per 100,000 tail fries are thrown in one of ripe egg yolk; After during the ripe egg yolk of throwing something and feeding is starched, fry is switched net cage, the powdery artifical compound feed of throwing something and feeding.
The present invention solves its technical problem and still takes following technical scheme to realize, method according to a kind of cyprinid fish inter-subfamily distant hybridization provided by the invention, comprise and select healthy, anosis, sexually matured male parent fish or maternal fish, then to maternal with carry out catalysis, adopt the manual method insemination, the unsticking hatching, to the juvenile fish bait acclimatization culture that hatches, wherein, maternal fish is selected blunt-snout gurnards for use, and the male parent fish is selected for use to stick up and chews red Culter fish.
The present invention solves its technical problem and can also take following technical scheme further to realize:
Aforementioned cross method wherein, carries out artificial catalysis to maternal fishball head gurnet, hastens parturition with 800 international unit human chorionic gonadtropin mixing ocyodinics with 4.0 μ g luteotropin releasing hormone d-ala analogs (LHRH-A2) with every kg fish body weight.
Aforementioned cross method behind the hybridization parent population artificial induced spawning, arrives effect and carries out artificial insemination during the time immediately, and wherein, artificial insemination adopts 1 tail raun to join 2 tail milters.
The present invention solves its technical problem and takes following technical scheme to realize again, method according to a kind of cyprinid fish inter-subfamily distant hybridization provided by the invention, comprise and select healthy, anosis, sexually matured male parent fish or maternal fish, then to maternal with carry out catalysis, adopt the manual method insemination, the unsticking hatching, to the juvenile fish bait acclimatization culture that hatches, wherein, maternal fish is selected for use, and the male parent fish is selected blunt-snout gurnards for use.
The present invention solves its technical problem and can also take following technical scheme further to realize:
Aforementioned cross method wherein, carries out artificial catalysis to maternal fishball head gurnet, with every kg fish body weight with 4.0 μ g luteotropin releasing hormone d-ala analog (LHRH-A
2) hasten parturition with 800 international unit human chorionic gonadtropin mixing ocyodinics.
The present invention solves its technical problem and takes following technical scheme to realize again, method according to a kind of cyprinid fish inter-subfamily distant hybridization provided by the invention, comprise and select healthy, anosis, sexually matured male parent fish or maternal fish, then to maternal with carry out catalysis, the insemination of employing manual method, the unsticking hatching, to the juvenile fish bait acclimatization culture that hatches, wherein maternal fish is selected fourth for use
Fish, the male parent fish is selected blunt-snout gurnards for use.
Aforementioned cross method, wherein, to maternal fish fourth
Fish carries out artificial catalysis, with every kg fish body weight with 5.0 μ g luteotropin releasing hormone d-ala analog (LHRH-A
2) hasten parturition with 800 international unit human chorionic gonadtropin mixing ocyodinics.
Aforementioned cross method behind the hybridization parent population artificial induced spawning, arrives effect and carries out artificial insemination during the time immediately, and wherein, artificial insemination adopts 1 tail raun to join 2 tail milters.
The present invention compared with prior art has apparent advantage and beneficial effect.
By above technical scheme as can be known, the present invention has following advantage at least:
Fish subfamily intermolecular hybrid combination of the present invention, the offspring that can obtain to have vitality in batches;
Fish subfamily intermolecular hybrid method of the present invention can improve exomixis rate and incubation rate greatly;
Cyprinid fish subfamily intermolecular hybrid method of the present invention can be hybridized the cultivation new fish kind;
The method that the present invention addresses, can improve the disease-resistant rate of hybrid, survival rate has reached the rate of catching, and can realize that production hybridization produces fry, thereby it compared with prior art has outstanding substantive distinguishing features and obvious improvement, in a word, the present invention can be rated as have novelty, the good technology of creativeness, practicality.
The specific embodiment of the present invention is provided in detail by following examples.
Embodiment
Below in conjunction with accompanying drawing and preferred embodiment, to according to embodiment provided by the invention, structure, feature and effect thereof, describe in detail as after.
A kind of method of cyprinid fish inter-subfamily distant hybridization, comprise and select healthy, anosis, sexually matured male parent fish or maternal fish, then to maternal with carry out catalysis, the insemination of employing manual method, the unsticking hatching is to the juvenile fish bait acclimatization culture that hatches, wherein, maternal fish is selected Cyprinus carpio L. Mirror for use, and the male parent fish is selected blunt-snout gurnards for use; Or maternal fish selects blunt-snout gurnards for use, and the male parent fish is selected for use to stick up and chews the snapper whitefish; Or maternal fish is selected fourth for use
Fish, the male parent fish is selected blunt-snout gurnards for use,
It is male parent (♂) or maternal (♀) that the parent of described three hybrid combinations selects with the megalobrama amblycephala, with mirror carp, stick up and chew red Culter and fourth
Hybridize, obtained above-mentioned three subfamily species hybrid offsprings, wherein megalobrama amblycephala shows huge breeding potentiality, mirror carp ♀ * megalobrama amblycephala ♂ combination, and fertilization rate is 87.5%, incubation rate is 83.0%; Megalobrama amblycephala ♀ * stick up and chew red Culter ♂ combination, fertilization rate is 62.7%, incubation rate is 80.1%; Fourth
♀ * megalobrama amblycephala ♂ hybrid combination, fertilization rate is 84.1%, incubation rate is 60%.；
The parent of aforementioned three hybrid combinations has adopted artificial induced spawning, wherein, ocyodinic is: luteotropin releasing hormone d-ala analog (LHRH-A2), maleic acid DOM (DOM) and human chorionic gonadtropin (abbreviation hormone), adopt 2 kinds or 3 kinds of hormones mixing formulations, and different fish are adopted different dosage form and dosage, and specifically: mirror carp is female, the every kg body weight of milter LHRH-A2 6.0 μ g+DOM 2.0mg+ hormones 1000 international units; Megalobrama amblycephala is female, the every kg body weight of milter LHRH-A2 4.0 μ g+ hormones 800 international units; Fourth
The every kg body weight of female, milter LHRH-A2 5.0 μ g+ hormones 800 international units; Stick up chew that red Culter is female, the every kg body weight of milter is with LHRH-A2 6.0 μ g+ hormones 1000 international units.Present technique adopts above-mentioned ocyodinic and dosage to make all that hybridization is female, the milter normal mature is laid eggs and obtains the higher fertilized egg of fertilization rate, assurance normally the carrying out of distant hybridization.
The parent of aforementioned three hybrid combinations has adopted artificial insemination and hatching, and artificial insemination arrives effect and carries out immediately during the time behind hybridization parent population artificial induced spawning, wherein, adopts 1 tail raun to join 2 tail milters, to improve fertilization rate;
Because used hybrid strain raun all is to produce viscid egg, after smart feritilization of ovum 3-5 minute, immediately fertilized egg is sticked to 90cm * 50cm of being positioned in advance under the depth of water 8-lOcm on the ovum net sheet, each net agreement that contracts a film or TV play to an actor or actress ten thousand of ovum 1-2;
After the artificial insemination process finishes, with the net sheet of ovum hang at area 12m
2Net cage in, the net sheet is dried up about about 10cm, net cage is arranged in the about 2 meters fishpond of the depth of water, the about 80cm of spacing between the net cage, about 5 days of about 20 ℃ of water temperatures, the fertilized egg on the net sheet was hatched fry, 3-5 days ripe egg yolk slurries of throwing something and feeding after incubating, per 100,000 tail fries feed one of ripe egg yolk; After 4-5 days, pour fry into new cage rearing, the powdery artifical compound feed of throwing something and feeding every day.
Mirror carp ♀ * megalobrama amblycephala ♂, the megalobrama amblycephala ♀ that the present invention obtains * stick up and chew red Culter ♂ and fourth
♀ * three subfamily intermolecular hybrid combinations of megalobrama amblycephala ♂ obtained the offspring with vitality in batches, and their anti-hybrid combination does not all obtain vital hybrid, wherein,
Mirror carp ♀ * megalobrama amblycephala ♂ combination, fertilization rate is 87.5%, incubation rate is 83.0%;
Megalobrama amblycephala ♀ * stick up and chew red Culter ♂ combination, fertilization rate is 62.7%, incubation rate is 80.1%;
Fourth
♀ * megalobrama amblycephala ♂ hybrid combination, fertilization rate is 84.1%, incubation rate is 60%.
The fish subfamily intermolecular hybrid of three combinations, its fertilization rate and incubation rate all are higher in the fish distant hybridization, wherein,
Mirror carp ♀ * megalobrama amblycephala ♂ combination obtains fry 1,708 ten thousand tails altogether,
Megalobrama amblycephala ♀ * stick up chews red Culter ♂ combination and obtains fry 60,000 tails altogether,
Fourth
♀ * megalobrama amblycephala ♂ combination obtains fry 3000 tails altogether.
Three subfamily intermolecular hybrids show that megalobrama amblycephala is that a distant hybridization coordinate force (affinity) is extremely strong, the breeding material with great potentiality, and father and mother originally can become the parent of breeding.
Three distant hybridization combination of the present invention, because it is hybrid survival rate height, disease-resistant, to work the rate of catching high, can standardize and produce the hybrid fry, therefore very high application value and DEVELOPMENT PROSPECT arranged, and simultaneously, has the possibility of cultivating new varieties.
The above, it only is preferred embodiment of the present invention, be not that the present invention is done any pro forma restriction, every foundation technical spirit of the present invention all still belongs in the scope of technical solution of the present invention any simple modification, equivalent variations and modification that above embodiment did.
Claims (10)
1. the method for a cyprinid fish inter-subfamily distant hybridization, comprise and select healthy, anosis, sexually matured male parent fish or maternal fish, then maternal fish is carried out catalysis, the insemination of employing manual method, the unsticking hatching is to the juvenile fish bait acclimatization culture that hatches, wherein, maternal fish is selected Cyprinus carpio L. Mirror for use, and the male parent fish is selected blunt-snout gurnards for use.
2. cross method according to claim 1 is characterized in that: maternal fish Cyprinus carpio L. Mirror is carried out artificial catalysis, with every kg fish body weight with 6.0 μ g luteotropin releasing hormone d-ala analog (LHRH-A
2) hasten parturition with 2.0mg maleic acid DOM (DOM) and 1000 international unit human chorionic gonadtropin mixing ocyodinics.
3. as cross method as described in the claim 2, behind the hybridization parent population artificial induced spawning, arrive effect and carry out artificial insemination during the time immediately, it is characterized in that: artificial insemination adopts 1 tail raun to join 2 tail milters.
4. as cross method as described in the claim 3, it is characterized in that: described unsticking hatching is when artificial insemination, after smart feritilization of ovum 3-5 minute, fertilized egg is sticked to be positioned in advance under the depth of water 8-10cm on the ovum net sheet;
After the artificial insemination, with the net sheet of ovum hang in net cage, and make about dried up 10cm of a net pitch of fins, net cage is arranged in the fishpond of about 2 meters of the depth of waters, and about the spacing 80cm between each net cage, water temperature is arranged on about 20 ℃, time is 5 days, and the fertilized egg on the net sheet is hatched fry.
5. as cross method as described in the claim 4, described juvenile fish bait acclimatization culture to hatching is characterized in that: to the fry that the hatches ripe egg yolk slurry of throwing something and feeding, during be 3-5 days, ripe egg yolk input ratio is that per 100,000 tail fries are thrown in one of ripe egg yolk; After during the ripe egg yolk of throwing something and feeding is starched, fry is switched net cage, the powdery artifical compound feed of throwing something and feeding.
6. the method for a cyprinid fish inter-subfamily distant hybridization, comprise and select healthy, anosis, sexually matured male parent fish or maternal fish, then to maternal with carry out catalysis, the insemination of employing manual method, the unsticking hatching is to the juvenile fish bait acclimatization culture that hatches, wherein, maternal fish is selected blunt-snout gurnards for use, and the male parent fish is selected for use to stick up and chews red Culter fish.
7. as cross method as described in the claim 6, it is characterized in that: maternal fishball head gurnet is carried out artificial catalysis, with every kg fish body weight with 4.0 μ g luteotropin releasing hormone d-ala analog (LHRH-A
2) hasten parturition with 800 international unit human chorionic gonadtropin mixing ocyodinics.
8. the method for a cyprinid fish inter-subfamily distant hybridization comprises and selects healthy, anosis, sexually matured male parent fish or maternal fish, then to maternal and carry out catalysis, the insemination of employing manual method, the unsticking hatching is to the juvenile fish bait acclimatization culture that hatches, wherein, maternal fish is selected fourth for use
, the male parent fish is selected blunt-snout gurnards for use.
9. as cross method as described in the claim 8, it is characterized in that: maternal fish fourth
Carry out artificial catalysis, with every kg fish body weight with 5.0 μ g luteotropin releasing hormone d-ala analog (LHRH-A
2) hasten parturition with 800 international unit human chorionic gonadtropin mixing ocyodinics.
10. as cross method as described in claim 7 or 9, behind the hybridization parent population artificial induced spawning, arrive effect and carry out artificial insemination during the time immediately, it is characterized in that: artificial insemination adopts 1 tail raun to join 2 tail milters.
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Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100464633C (en) * | 2006-09-18 | 2009-03-04 | 浙江省淡水水产研究所 | Erythtoculer ilishaefornis and megajobrama amblycephala crossbreeding method |
| CN101884314A (en) * | 2010-07-26 | 2010-11-17 | 湖南师范大学 | Method for inter-subfamily distant hybridization of grass carp and megalobrama amblycephala |
| CN101341859B (en) * | 2008-08-29 | 2010-12-01 | 湖南师范大学 | Distant hybridization method for megalobrama amblycephala and yellow catfish |
| CN102972319A (en) * | 2012-12-12 | 2013-03-20 | 湖南师范大学 | Method for establishment of hybrid strain between bluntsnout bream and topmouth culter and breeding method of topmouth bream |
| CN104221952A (en) * | 2013-12-26 | 2014-12-24 | 江苏省农业科学院宿迁农科所 | Artificial reproduction method of chalcalburnus chalcoides aralensis |
| CN104585083A (en) * | 2014-12-15 | 2015-05-06 | 湖南师范大学 | Method for distant hybridization between subfamilies of cyprinus carpio and megalobrama amblycephala |
| CN105284685A (en) * | 2015-11-04 | 2016-02-03 | 台江县国营水产养殖场 | Crossbreeding method for Qingshui river carps and Furui carps |
| CN105684962A (en) * | 2015-11-17 | 2016-06-22 | 浙江省淡水水产研究所 | A culter alburnus and triangular bream crossbreed preparation method |
| CN105850812A (en) * | 2016-04-28 | 2016-08-17 | 上海海洋大学 | Method for constructing novel Megalobrama-culter backcross strain with high growth rate and low-oxygen resistance |
| CN106550909A (en) * | 2016-10-31 | 2017-04-05 | 湖南师范大学 | The method of Cyprinus carpio L. and megalobrame amblycephala subfamily distant hydridization |
| CN110063277A (en) * | 2019-05-27 | 2019-07-30 | 绥阳县渔缘水产养殖专业合作社 | The cross breeding method of Songpu mirror carp and blueness Tian Tianyu |
| CN112400761A (en) * | 2020-12-04 | 2021-02-26 | 中国科学院昆明动物研究所 | Sinocyclocheilus grahami and carp hybridization method |
| CN114793959A (en) * | 2022-05-06 | 2022-07-29 | 昆明市水产科学研究所 | Artificial whitebait propagation method for improving survival rate of postpartum parent fish |
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- 2003-09-02 CN CNB03144234XA patent/CN1201654C/en not_active Expired - Fee Related
Cited By (21)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN100464633C (en) * | 2006-09-18 | 2009-03-04 | 浙江省淡水水产研究所 | Erythtoculer ilishaefornis and megajobrama amblycephala crossbreeding method |
| CN101341859B (en) * | 2008-08-29 | 2010-12-01 | 湖南师范大学 | Distant hybridization method for megalobrama amblycephala and yellow catfish |
| CN101884314A (en) * | 2010-07-26 | 2010-11-17 | 湖南师范大学 | Method for inter-subfamily distant hybridization of grass carp and megalobrama amblycephala |
| CN101884314B (en) * | 2010-07-26 | 2011-11-23 | 湖南师范大学 | Method for inter-subfamily distant hybridization of grass carp and megalobrama amblycephala |
| CN102972319A (en) * | 2012-12-12 | 2013-03-20 | 湖南师范大学 | Method for establishment of hybrid strain between bluntsnout bream and topmouth culter and breeding method of topmouth bream |
| CN104221952B (en) * | 2013-12-26 | 2016-08-17 | 江苏省农业科学院宿迁农科所 | A kind of Chalcalbumus chalcoides aralensis artificial fecundation method |
| CN104221952A (en) * | 2013-12-26 | 2014-12-24 | 江苏省农业科学院宿迁农科所 | Artificial reproduction method of chalcalburnus chalcoides aralensis |
| CN104585083A (en) * | 2014-12-15 | 2015-05-06 | 湖南师范大学 | Method for distant hybridization between subfamilies of cyprinus carpio and megalobrama amblycephala |
| CN104585083B (en) * | 2014-12-15 | 2017-04-26 | 湖南师范大学 | Method for distant hybridization between subfamilies of cyprinus carpio and megalobrama amblycephala |
| CN105284685A (en) * | 2015-11-04 | 2016-02-03 | 台江县国营水产养殖场 | Crossbreeding method for Qingshui river carps and Furui carps |
| CN105284685B (en) * | 2015-11-04 | 2018-02-16 | 台江县国营水产养殖场 | A kind of Qingshuijiang carp and the auspicious carp cross breeding method of good fortune |
| CN105684962A (en) * | 2015-11-17 | 2016-06-22 | 浙江省淡水水产研究所 | A culter alburnus and triangular bream crossbreed preparation method |
| CN105684962B (en) * | 2015-11-17 | 2018-05-11 | 浙江省淡水水产研究所 | One kind sticks up mouth Culter and triangular bream hybrid preparation method |
| CN105850812A (en) * | 2016-04-28 | 2016-08-17 | 上海海洋大学 | Method for constructing novel Megalobrama-culter backcross strain with high growth rate and low-oxygen resistance |
| CN105850812B (en) * | 2016-04-28 | 2018-11-06 | 上海海洋大学 | A kind of construction method of triangular bream Culter backcrossing new lines high, lower oxygen concentration resistance with growth rate |
| CN106550909A (en) * | 2016-10-31 | 2017-04-05 | 湖南师范大学 | The method of Cyprinus carpio L. and megalobrame amblycephala subfamily distant hydridization |
| CN106550909B (en) * | 2016-10-31 | 2019-07-26 | 湖南师范大学 | The method of fancy carp and megalobrame amblycephala subfamily distant hydridization |
| CN110063277A (en) * | 2019-05-27 | 2019-07-30 | 绥阳县渔缘水产养殖专业合作社 | The cross breeding method of Songpu mirror carp and blueness Tian Tianyu |
| CN112400761A (en) * | 2020-12-04 | 2021-02-26 | 中国科学院昆明动物研究所 | Sinocyclocheilus grahami and carp hybridization method |
| CN114793959A (en) * | 2022-05-06 | 2022-07-29 | 昆明市水产科学研究所 | Artificial whitebait propagation method for improving survival rate of postpartum parent fish |
| CN114793959B (en) * | 2022-05-06 | 2023-03-14 | 昆明市水产科学研究所 | Whitebait artificial propagation method for improving survival rate of postpartum parent fish |
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| CN1201654C (en) | 2005-05-18 |
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