CN110447577A - A kind of Odontobulis mpotamophila scale efficient propagation method based in due course temperature control - Google Patents
A kind of Odontobulis mpotamophila scale efficient propagation method based in due course temperature control Download PDFInfo
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- 230000012447 hatching Effects 0.000 claims abstract description 33
- 241000251468 Actinopterygii Species 0.000 claims abstract description 24
- 210000004681 ovum Anatomy 0.000 claims abstract description 22
- 230000000384 rearing effect Effects 0.000 claims abstract description 21
- 102000002322 Egg Proteins Human genes 0.000 claims abstract description 18
- 108010000912 Egg Proteins Proteins 0.000 claims abstract description 18
- 235000013601 eggs Nutrition 0.000 claims abstract description 10
- 230000013020 embryo development Effects 0.000 claims abstract description 10
- 241001113556 Elodea Species 0.000 claims abstract description 9
- 241000238557 Decapoda Species 0.000 claims abstract description 8
- XNOPRXBHLZRZKH-MQYCRUOZSA-N oxytocin Chemical compound C([C@H]1C(=O)N[C@H](C(N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CSSC[C@H](N)C(=O)N1)C(=O)N1C(CCC1)C(=O)N[C@@H](CC(C)C)C(=O)NCC(N)=O)=O)[C@@H](C)CC)C1=CC=C(O)C=C1 XNOPRXBHLZRZKH-MQYCRUOZSA-N 0.000 claims abstract description 7
- 229940082408 oxytocin injection Drugs 0.000 claims abstract description 7
- 238000003306 harvesting Methods 0.000 claims abstract description 5
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 8
- ORTYMGHCFWKXHO-UHFFFAOYSA-N diethadione Chemical compound CCC1(CC)COC(=O)NC1=O ORTYMGHCFWKXHO-UHFFFAOYSA-N 0.000 claims description 8
- 229940090044 injection Drugs 0.000 claims description 6
- 238000002347 injection Methods 0.000 claims description 6
- 239000007924 injection Substances 0.000 claims description 6
- 239000012528 membrane Substances 0.000 claims description 6
- 230000018109 developmental process Effects 0.000 claims description 5
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims description 4
- 241000907635 Farfantepenaeus duorarum Species 0.000 claims description 4
- 206010062767 Hypophysitis Diseases 0.000 claims description 4
- 229930003268 Vitamin C Natural products 0.000 claims description 4
- 238000005273 aeration Methods 0.000 claims description 4
- 229960003675 diethadione Drugs 0.000 claims description 4
- 238000011017 operating method Methods 0.000 claims description 4
- 210000000006 pectoral fin Anatomy 0.000 claims description 4
- 235000019154 vitamin C Nutrition 0.000 claims description 4
- 239000011718 vitamin C Substances 0.000 claims description 4
- 238000010438 heat treatment Methods 0.000 claims description 3
- 230000000694 effects Effects 0.000 abstract description 4
- 230000002159 abnormal effect Effects 0.000 abstract description 3
- 230000002411 adverse Effects 0.000 abstract description 2
- 230000006353 environmental stress Effects 0.000 abstract description 2
- 235000013339 cereals Nutrition 0.000 description 11
- 230000017448 oviposition Effects 0.000 description 7
- ODINCKMPIJJUCX-UHFFFAOYSA-N Calcium oxide Chemical compound [Ca]=O ODINCKMPIJJUCX-UHFFFAOYSA-N 0.000 description 4
- 230000001488 breeding effect Effects 0.000 description 3
- 241001669679 Eleotris Species 0.000 description 2
- 241001669656 Odontobutis obscura Species 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 235000012255 calcium oxide Nutrition 0.000 description 2
- 239000000292 calcium oxide Substances 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- 239000013505 freshwater Substances 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 239000008239 natural water Substances 0.000 description 2
- 230000006641 stabilisation Effects 0.000 description 2
- 238000011105 stabilization Methods 0.000 description 2
- 241000251730 Chondrichthyes Species 0.000 description 1
- 241000583629 Cypridopsis vidua Species 0.000 description 1
- 241001670009 Eleotridae Species 0.000 description 1
- 241000969797 Odontobutis potamophila Species 0.000 description 1
- 241000276618 Perciformes Species 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
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- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 210000002257 embryonic structure Anatomy 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
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- 238000006213 oxygenation reaction Methods 0.000 description 1
- 230000032696 parturition Effects 0.000 description 1
- 238000009304 pastoral farming Methods 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 238000004886 process control Methods 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/17—Hatching, e.g. incubators
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/32—Biological treatment of water, waste water, or sewage characterised by the animals or plants used, e.g. algae
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The Odontobulis mpotamophila biological control method based in due course temperature control that the present invention relates to a kind of.Specific steps are as follows: the antenatal cultivation of (1) parent population, transplants waterweed in parent fish rearing pond, the small long-armed shrimp of China is launched, while launching Odontobulis mpotamophila parent by sex ration, (2) Building environment launches fresh and alive spiral shell, to purify water；(3) artificial spawning nest is set, forms the artificial spawning nest of tubulose with the opposite binding of two pieces of arc-shaped grey tile pieces；(4) artificial induced spawning finds to be stained with ovum grain in artificial laying nest, drains water, and spawning pond is arranged in harvesting parent population, gives parent population oxytocin injection；(5) temperature control hatching in due course, embryonic development to neurula stage move into indoor temperature control hatching in time.Method of the invention reduces incubating oosperm environmental stress early period to the adverse effect of embryonic development；More whole temperature control hatching can save energy 73%, reduces abnormal rate 8%, improves hatchability of fertile eggs 11%, can shorten 5 days~7 days compared with room temperature hatching.
Description
Technical field
The invention belongs to technical field of aquaculture, are related to a kind of Odontobulis mpotamophila propagation method, and in particular to Yi Zhongji
In the Odontobulis mpotamophila scale efficient propagation method of in due course temperature control.
Background technique
Odontobulis mpotamophila (Odontobutis potamophila Günther), it is subordinate to Perciformes, Eleotridae, Odontobutis obscura
Belong to, is commonly called as " brave head shark ", " idiot fish ", " soil step fish ", is distributed in Yangtze River in China middle and lower reaches and its tributary, Qiantang River and Fujian
River Drainage etc. is Chinese unique wheat.By favor, especially 2010 due to its meat flavour is delicious, nutritive value and medical value are high
It after being known as " the first dish of Expo " in year Shanghai World's Fair, even more gains considerable fame, price is also constantly soaring, Shanghai, Hangzhou
Area reaches as high as 150~200 yuan/kilogram.
Currently, Odontobulis mpotamophila marketable fish is caught mainly from natural water body and pond artificial fecundation, with Odontobulis mpotamophila seedling
Kind artificial propagation and cultural technique research deepen continuously with what is practiced, and corresponding technological invention also emerges in succession.At this stage, rivers and creeks
Difficult point and main problem in Odontobutis obscura biological control have: one, the natural spawning time excessively disperses, complete Ecology breeding effect
Rate is low.It has a surplus even if areal also will continue January with parent's egg-laying time that pond is cultivated, and climate condition of laying eggs influences
Greatly.Two, incubating oosperm overlong time, hatching rate is low in the case of no parent population protects ovum.Under the conditions of artificial propagation, natural water hatching
Water temperature is low, and the incubating oosperm membrane time, water mold occurrence probability was big generally at 20 days~30 days, since Odontobulis mpotamophila is nest
Oviposition, the distribution of ovum grain are more concentrated, once local infection water mold, which can spread periphery normal developing embryos, causes necrosis in flakes, even
It is annihilated.Fertilized eggs whole process temperature control Lotic hatching is such as used, though incubation cycle can be shortened, reduces water mold, whole process control
Energy consumption is high, at high cost for temperature, and since Odontobulis mpotamophila embryonic development initial stage is more sensitive to environment, it is abnormal embryonic development easily occur
The problems such as shape, not high hatching rate.
Summary of the invention
In order to realize that the scale of Odontobulis mpotamophila seed is efficiently bred, the present invention provides a kind of river based in due course temperature control
Chuansha sleeper biological control method.
A kind of operating procedure of the Odontobulis mpotamophila biological control method based in due course temperature control is as follows:
(1) the antenatal cultivation of parent population
December to next year 2 months, waterweed is transplanted in the parent fish rearing pond of depth of water 15cm~20cm, after two weeks, is filled to water
Deep 50cm~60cm, every 667m2Launch 35kg~45kg bait shrimp, while by sex ration 1:1.1~1.2 launch 75kg~
100kg Odontobulis mpotamophila parent, wherein raun individual weight 35g~45g, milter individual weight 60g~75g；
(2) Building environment
Late March launches spiral shell into parent fish rearing pond, to purify water；
(3) artificial spawning nest is set
At the beginning of 4 months, 3~5 artificial laying nests are set in parent fish rearing pond, are checked daily in artificial spawning nest that whether there is or not ovum grain is attached
；
When being stained with ovum grain in artificial spawning nest to be found, water is drained in time, and harvesting parent population simultaneously temporarily cultivates parent population in net cage
In；Then 1/m is pressed2Artificial spawning nest is evenly arranged on pond surrounding and forms spawning pond by the amount of artificial spawning nest, and is suspended in
At 10~12cm of bottom of pond；It is filled the water to spawning pond, water level restores to original position；
(4) artificial induced spawning
Parent population is picked up, using pectoral fin base portion injection method oxytocin injection, every kg raun presses hypophysis (PG) 8mg+dioxone (DOM)
12mg+vitamin C (Vc) 300mg is injected, and milter halves；The parent population of oxytocin injection is put back into spawning pond, 48h~
After 60h, parent population starts to lay eggs；
(5) temperature control hatching in due course
Routine observation development of fertilized ova situation, when observing that embryonic development to the neurula stage, will adhere to the artificial of ovum grain in time
Laying nest moves into indoor hatchery, and the depth of water of hatchery is 30cm~40cm, flow velocity is 0.3m/s~0.5m/s, hatching density
For 80,000/m2~10 ten thousand/m2, hatching bottom of pond portion is by 2W/m2~3W/m2Arrange nanometer micropore pipe aeration, slow gradient increased temperature
It is 26 DEG C~28 DEG C to hatching water temperature, by 2 days~3 days, prelarva membrane completed hatching.
The technical step further limited is as follows:
In step (1), the area in the parent fish rearing pond is 667m2~2000m2, waterweed planted area accounts for parent fish rearing pond
Area 1/3~1/2.
In step (1), the bait shrimp is the small long-armed shrimp of China.
In step (2), the injected volume of spiral shell is every 667m2Launch 200kg~300kg.
In step (3), artificial spawning nest forms tubular structure, both ends nozzle shape by the opposite binding of two pieces of arc-shaped grey tile pieces
At hole, and hole is parallel to the water surface, is suspended in away from bottom of pond 1cm0~12cm；The length of the arc-shaped grey tile piece is 25cm
~30cm, width are 10cm~15cm.
In step (5), heating gradient is 0.5 DEG C/h~1 DEG C/h.
Compared with prior art, advantageous effects of the invention embody in the following areas:
1, the present invention can be dropped by the antenatal cultivation of parent population, Building environment, laying nest setting, artificial induced spawning and the hatching of in due course temperature control
The low antenatal cultivation cost of Odontobulis mpotamophila biological control improves parent population oviposition and hatching water quality stability early period, in abundant honor
Again and using on the basis of parent population reproductive habit, in due course temperature control is taken to hatch, reduces environmental stress incubating oosperm early period to embryo
The adverse effect that fetal hair is educated.Compared with the hatching of whole temperature control, the present invention can save energy 73%, reduce abnormal rate 8%, improve fertilization
Egg hatching rate 11%.
2, the current Odontobulis mpotamophila parent fish rearing stage is mostly using freshwater shrimp as bait, and winter, small dimension freshwater shrimp was often with seed shrimp
Form sale, price is high, invisible to improve parent fish rearing cost, and it is small to use the China that disposition is more mild, price is more cheap instead
For long-armed shrimp as feed source, size specification is moderate, and parent population grazing rate is higher, can get more preferable cultivate effect.
3, spiral shell is launched in parent fish rearing pond or spawning pond, on the one hand, utilize spiral shell purification water body ability, improve water
Body transparency promotes water plant growth, maintains water stabilization, is conducive to hatch fertilized eggs early period after hastening parturition；On the other hand, rivers and creeks is husky
Sleeper mating period, also exactly when spiral shell breeding, the small spiral shell of breeding is the agreeable to the taste bait of parent fish rearing.
4, the present invention uses shaggy grey tile piece manufacturing artificial laying nest, and adhesive force is strong, and ovum grain is not easily to fall off, improves
Hatchability of fertile eggs.
5, after artificial induced spawning of the invention, parent population is taken freely to match oviposition, actively instigates fin when protecting ovum using male parent population
Item, development of eating stop the habits such as fish-egg, play parent population oxygenation, reject the functions such as bad ovum, prevent water mold from occurring, sprawling.
6, embryonic development moves into 26 DEG C~28 DEG C hatcheries of water temperature to the neurula stage and hatches, and is tended in embryonic development
Under the premise of stabilization, by temperature control, shortens incubation cycle, improve hatching efficiency.The incubating oosperm membrane time 15 days of the present invention
~16 days, 5 days~7 days can be shortened compared with room temperature hatching.
Specific embodiment
Combined with specific embodiments below, the present invention is further described.
Embodiment 1
A kind of Odontobulis mpotamophila biological control operating procedure based in due course temperature control is as follows:
(1) the antenatal cultivation of parent population
The area 1000m in parent fish rearing pond2, sterilized with quick lime 400kg pond cleaning on November 23rd, 2016；December 10,
Start to fill the water 15cm~20cm, transplant waterweed, waterweed planted area accounts for the 1/2 of pond area.
After 15 days, it is filled to depth of water 50cm~60cm, launches the small long-armed shrimp 35kg of China, while throwing by sex ration 1:1.1
Put 75kg Odontobulis mpotamophila parent, raun individual counterpoise 41g, milter individual counterpoise 73g.
(2) Building environment
It launched fresh and alive spiral shell 400kg March 25, is sprinkling upon parent fish rearing pond surrounding, uniformly to purify water.
(3) artificial spawning nest is set
April 2,5 artificial laying nests were set in parent fish rearing pond, to observe parent population oviposition situation；By long 25cm, width 10cm
The opposite binding of two pieces of arc-shaped grey tile pieces form the artificial spawning nest of tubulose, both ends nozzle forms hole and is parallel to the water surface, people
Work laying nest is suspended in away from bottom of pond 10cm.Next morning begins, and daily lifts artificial spawning nest 1 time, it is attached to check for ovum grain
.
(4) artificial induced spawning
It April 9 found that the grey tile on piece of artificial spawning nest at 2 was stained with ovum grain, and drained water in time, harvesting parent population is simultaneously temporarily supported
It grows in 4m × 2m × 2m net cage；Then 1000 artificial laying nests are evenly arranged on pond surrounding and form spawning pond, to oviposition
Pond water filling, water level restore to original position.
Started artificial induced spawning April 10, using pectoral fin base portion injection method oxytocin injection, every kg raun presses hypophysis (PG)
8mg+dioxone (DOM) 12mg+vitamin C (Vc) 300mg is injected, and every endnote penetrates 0.5ml, and milter halves.Injection
Afterwards, parent population is put back into spawning pond.
(5) temperature control hatching in due course
April 12 started to lay eggs, daily routine observation development of fertilized ova situation；April 25, observe embryonic development to neurula
Stage in time moves into the laying nest for being developed to stage attachment ovum grain in indoor hatchery, and indoor hatching water keeps the depth of water
80,000 38cm, flow velocity 0.4m/s, hatching density/m2, hatching bottom of pond portion press 2W/m2Arrange nanometer micropore pipe aeration, using boiler
1 DEG C/h of slow gradient increased temperature is pressed in heating, and hatching water temperature is finally stable at 26 DEG C.April 28, prelarva membrane are total to hatch
1,000,000 tail of prelarva.
Embodiment 2
A kind of Odontobulis mpotamophila biological control operating procedure based in due course temperature control is as follows:
(1) the antenatal cultivation of parent population
Parent fish rearing pool area 1300m2, sterilized with quick lime 500kg pond cleaning on December 12nd, 2017；It December 29, opens
Begin water filling 15cm~20cm, transplants waterweed, waterweed planted area accounts for the 1/2 of pond area.
It is filled to depth of water 50cm~60cm on January 15th, 2018, launches the small long-armed shrimp 45kg of China, while pressing sex ration
1:1.1 launches Odontobulis mpotamophila parent 100kg, raun individual counterpoise 35g, milter individual counterpoise 61g.
(2) Building environment
It launched fresh and alive spiral shell 550kg March 28, is uniformly sprinkling upon parent fish rearing pond surrounding.
(3) artificial spawning nest is set
April 4,5 artificial laying nests were set in parent fish rearing pond, to observe parent population oviposition situation, by long 25cm, width 10cm
The opposite binding of two pieces of arc-shaped grey tile pieces form the artificial spawning nest of tubulose, both ends nozzle forms hole and is parallel to the water surface, people
Work laying nest is suspended in away from bottom of pond 10cm.Next morning begins, and daily lifts artificial spawning nest 1 time, it is attached to check for ovum grain
.
(4) artificial induced spawning
It April 11 found that the grey tile on piece of artificial spawning nest at 1 was stained with ovum grain, and drained water on April 13, harvesting parent population is simultaneously temporary
When cultivation in 4m × 2m × 2m net cage；Then 1300 artificial laying nests are evenly arranged on pond surrounding and form spawning pond, given
Spawning pond water filling, water level restore to original position.
Started artificial induced spawning April 14, using pectoral fin base portion injection method oxytocin injection, every kg raun presses hypophysis (PG)
8mg+dioxone (DOM) 12mg+vitamin C (Vc) 300mg is injected, and every endnote penetrates 0.5ml, and milter halves.Injection
Afterwards, parent population is put back into spawning pond.
(5) temperature control hatching in due course
April 16 started to lay eggs, daily routine observation development of fertilized ova situation；April 28, observe embryonic development to neurula
Stage in time moves into the artificial spawning nest for being developed to stage attachment ovum grain in indoor hatchery, and indoor hatchery keeps water
90,000 deep 35cm, flow velocity 0.4m/s, hatching density/m2, hatching bottom of pond portion press 3W/m2Arrange nanometer micropore pipe aeration, 1 DEG C/small
When slow gradient increased temperature, hatching water temperature is finally stable at 27 DEG C.May 1, prelarva membrane are total to hatch 1,200,000 tail of prelarva.
Claims (7)
1. a kind of Odontobulis mpotamophila biological control method based in due course temperature control, it is characterised in that operating procedure is as follows:
(1) the antenatal cultivation of parent population
December to next year 2 months, waterweed is transplanted in the parent fish rearing pond of depth of water 15cm~20cm, after two weeks, is filled to water
Deep 50cm~60cm, every 667m2Launch 35kg~45kg bait shrimp, while by sex ration 1:1.1~1.2 launch 75kg~
100kg Odontobulis mpotamophila parent, wherein raun individual weight 35g~45g, milter individual weight 60g~75g；
(2) Building environment
Late March launches spiral shell into parent fish rearing pond, to purify water；
(3) artificial spawning nest is set
At the beginning of 4 months, 3~5 artificial laying nests are set in parent fish rearing pond, are checked daily in artificial spawning nest that whether there is or not ovum grain is attached
；
When being stained with ovum grain in artificial spawning nest to be found, water is drained in time, and harvesting parent population simultaneously temporarily cultivates parent population in net cage
In；Then 1/m is pressed2Artificial spawning nest is evenly arranged on pond surrounding and forms spawning pond by the amount of artificial spawning nest, and is suspended in
At 10~12cm of bottom of pond；It is filled the water to spawning pond, water level restores to original position；
(4) artificial induced spawning
Parent population is picked up, using pectoral fin base portion injection method oxytocin injection, every kg raun presses hypophysis (PG) 8mg+dioxone (DOM)
12mg+vitamin C (Vc) 300mg is injected, and milter halves；The parent population of oxytocin injection is put back into spawning pond, 48h~
After 60h, parent population starts to lay eggs；
(5) temperature control hatching in due course
Routine observation development of fertilized ova situation, when observing that embryonic development to the neurula stage, will adhere to the artificial of ovum grain in time
Laying nest moves into indoor hatchery, and the depth of water of hatchery is 30cm~40cm, flow velocity is 0.3m/s~0.5m/s, hatching density
For 80,000/m2~10 ten thousand/m2, hatching bottom of pond portion is by 2W/m2~3W/m2Arrange nanometer micropore pipe aeration, slow gradient increased temperature
It is 26 DEG C~28 DEG C to hatching water temperature, by 2 days~3 days, prelarva membrane completed hatching.
2. a kind of Odontobulis mpotamophila scale efficient propagation method based in due course temperature control according to claim 1, feature
Be: in step (1), the area in the parent fish rearing pond is 667m2~2000m2, waterweed planted area accounts for parent fish rearing pond
Area 1/3~1/2.
3. a kind of Odontobulis mpotamophila scale efficient propagation method based in due course temperature control according to claim 1, feature
Be: in step (1), the bait shrimp is the small long-armed shrimp of China.
4. a kind of Odontobulis mpotamophila scale efficient propagation method based in due course temperature control according to claim 1, feature
Be: in step (2), the injected volume of spiral shell is every 667m2Launch 200kg~300kg.
5. a kind of Odontobulis mpotamophila scale efficient propagation method based in due course temperature control according to claim 1, feature
Be: in step (3), artificial spawning nest forms tubular structure by the opposite binding of two pieces of arc-shaped grey tile pieces, and both ends nozzle is formed
Hole, and hole is parallel to the water surface, is suspended in away from bottom of pond 10cm~12cm.
6. a kind of Odontobulis mpotamophila scale efficient propagation method based in due course temperature control according to claim 5, feature
Be: the length of the arc-shaped grey tile piece is 25cm~30cm, width is 10cm~15cm.
7. a kind of Odontobulis mpotamophila scale efficient propagation method based in due course temperature control according to claim 1, feature
Be: in step (5), heating gradient is 0.5 DEG C/h~1 DEG C/h.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111955386A (en) * | 2020-07-26 | 2020-11-20 | 长江水利委员会长江科学院 | Artificial breeding method of Qinghai-Tibet plateau small-head nojirimus fish |
| CN114568356A (en) * | 2022-03-17 | 2022-06-03 | 西藏自治区农牧科学院水产科学研究所 | Method for large-scale cultivation of salmon fries in eastern Asian province |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1846480A (en) * | 2006-04-14 | 2006-10-18 | 浙江省淡水水产研究所 | Artificial breeding method and incubator for shatang carp |
| CN102499147A (en) * | 2011-11-01 | 2012-06-20 | 苏州市水产研究所有限公司 | Odontobutis obscura artificial propagation method |
| CN103493767A (en) * | 2013-10-11 | 2014-01-08 | 江苏省淡水水产研究所 | Cross breeding method of odontobutis potamophila and odontobutis yaluensis |
| WO2016168898A1 (en) * | 2015-04-22 | 2016-10-27 | Booth Donald Allan | Fish egg incubator |
| CN108739535A (en) * | 2018-05-07 | 2018-11-06 | 江苏中洋集团股份有限公司 | A kind of artificial fecundation method of Odontobulis mpotamophila |
-
2019
- 2019-09-24 CN CN201910906380.8A patent/CN110447577A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1846480A (en) * | 2006-04-14 | 2006-10-18 | 浙江省淡水水产研究所 | Artificial breeding method and incubator for shatang carp |
| CN102499147A (en) * | 2011-11-01 | 2012-06-20 | 苏州市水产研究所有限公司 | Odontobutis obscura artificial propagation method |
| CN103493767A (en) * | 2013-10-11 | 2014-01-08 | 江苏省淡水水产研究所 | Cross breeding method of odontobutis potamophila and odontobutis yaluensis |
| WO2016168898A1 (en) * | 2015-04-22 | 2016-10-27 | Booth Donald Allan | Fish egg incubator |
| CN108739535A (en) * | 2018-05-07 | 2018-11-06 | 江苏中洋集团股份有限公司 | A kind of artificial fecundation method of Odontobulis mpotamophila |
Non-Patent Citations (3)
| Title |
|---|
| 朱述淦 等: "河川沙塘鳢仿生态繁育技术试验", 《江西水产科技》 * |
| 熊良伟 等: "沙塘鳢规模化繁殖技术", 《科学养鱼》 * |
| 王金胜 等: "沙塘鳢人工繁育技术", 《渔业致富指南》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111955386A (en) * | 2020-07-26 | 2020-11-20 | 长江水利委员会长江科学院 | Artificial breeding method of Qinghai-Tibet plateau small-head nojirimus fish |
| CN114568356A (en) * | 2022-03-17 | 2022-06-03 | 西藏自治区农牧科学院水产科学研究所 | Method for large-scale cultivation of salmon fries in eastern Asian province |
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