CN103858794A - Takifugu obscurus fry breading method through application of small fertilized eggs - Google Patents
Takifugu obscurus fry breading method through application of small fertilized eggs Download PDFInfo
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- CN103858794A CN103858794A CN201210549182.9A CN201210549182A CN103858794A CN 103858794 A CN103858794 A CN 103858794A CN 201210549182 A CN201210549182 A CN 201210549182A CN 103858794 A CN103858794 A CN 103858794A
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Abstract
Disclosed is a Takifugu obscurus fry breading method through the application of small fertilized eggs. The Takifugu obscurus fry breading method through the application of the small fertilized eggs is composed of steps of hatching of fertilized egg, fry putting, inflation, temperature control, water quality control, pond dividing and bait feeding and is characterized in that the small fertilized eggs are put in a hatching pond with a conical bottom, wherein the density of the small fertilized eggs is 80000-100000 entities per cubic meter; the hatching pond is inflated in a still water mode, the water temperature is 21-22 DEG C, the illumination is 800-1000x and water is changed one time each day; newly-hatched fry are moved to a fry breeding pond after being temporarily bred in the hatching pond for two days, the density of the put fry is 10000 entities per cubic meter, and bottom suction, pollution discharge and water changing are performed on the fourth day, pond dividing is performed one time when the newly-hatched fry are bred for 18 and 25 days respectively; initial bait and SS type freshwater rotifers are fed when the fry are bred for 2-4 days, small freshwater rotifers are fed when the fry are bred for 5-7 days, middle-sized freshwater rotifers are fed when the fry are bred for 8-11 days, and the sizes of the fed creature bait increases as the fry grow; when the total length of the fry is 0.9-1.8 cm, the fry are moved to a pond from the breeding pond.
Description
Technical field
The present invention relates to aquaculture, the technology of especially utilizing the small-sized fertilized egg of fugu obscurus to grow seedlings.
Background technology
Fugu obscurus (
takifugu obscurus) belong to Tetraodontiformes, Puffer suborder, Molidae, Fugu belongs to, and is the important fishery resources of China's Water area of Changjiang River, has very high nutritive value and medical value.Its muscle is pure white as white, and meat flavour fat is beautiful, fresh and tender good to eat, is rich in protein, nutritious, is described as first of " the Changjiang river three delicacies ".Just there is the traditional habit of food river Puffer fish in China from ancient times, Puffer fish is regarded as the treasure on dining table always in river, and liver and the ovary of river Puffer fish can extract fugutoxin, are used for the treatment of neuralgia, spasm, bed-wetting etc., and therefore fugu obscurus economic worth and medical value are very high.Fugu obscurus is the principal item of China's river Puffer fish culture, and its cultured output is large, obtains every year economic benefit and reaches several hundred million units.River Puffer fish culture industry (particularly fugu obscurus) flourish, market is huge to the demand of fugu obscurus seed.
At present, the conventional artificial propagation of fugu obscurus and rearing of fingerling are relatively ripe, survival rate is all higher, but fugu obscurus artificial propagation hasten parturition and spawning and fertilization process in, tend to the fertilized egg that occurs that a part of individual relative is less, the ovum footpath 0.95-1.05mm of small-sized ovum, the ovum footpath >1.05 of normal ovum, the individuality of the newly hatched larvae after small-sized incubating oosperm is also relatively little, the newly hatched larvae total length of small-sized fertilized egg is the normal newly hatched larvae total length of 2.60-2.70mm(>2.75mm), this causes certain difficulty to seed rearing, the rearing of fingerling following conventional lines can cause low-down seed rearing survival rate, just need a set of special cultivation technology so small-sized fugu obscurus incubating oosperm newly hatched larvae is out cultivated into the black prelarva seedling of total length 1cm.
Summary of the invention
The object of this invention is to provide a kind of technology of utilizing the small-sized fertilized egg of fugu obscurus to cultivate fry.
Technical scheme of the present invention by incubating oosperm, put seedling, inflation, water temperature control, water quality control, point pond and bait feeding cultivating technique and form, it is characterized in that oosperm hatching technique is that small-sized fertilized egg is put into hatchery, triploidy rate is controlled at ten thousand/m of 8-10
3, hatchery is practical water body 0.5m
3conical bottom cement pit, adopt hydrostatic inflation to make Chi Shuicheng boiling shape, water temperature is 21-22 ℃, intensity of illumination 800-1000lx, and daytime is with the bilayer film shading of sheltering from heat or light, the light filling of turning on light evening, change water every day 1 time, change water 4/5 at every turn, through 100 hours beginning ruptures of membranes, after beginning rupture of membranes, through 24 hours, just there is more than 98% normotrophic embryo rupture of membranes; Putting seedling is that newly hatched larvae is supported temporarily after 2 days in hatchery, moves into seed rearing pond, and putting seedling density is 1.0 ten thousand tails/m
3putting seedling water level is 40cm, beginning in the 2nd day progressively adds water until fill it up with, within the 4th day, start to inhale bottom blowdown and change water, inhale 1 time, the end later every day, change water 2/3 every other day, the temperature difference in water temperature and the hatchery in seed rearing pond can not exceed 0.2 ℃, heats up 1.0 ℃ every 24 hours later, rise to 24.5 ± 0.5 ℃ of water temperatures, while putting seedling, the density of gas exhausting stone is 1.5/m
2, reduce to gradually 1/m of density
2; Newly hatched larvae to the 18 ages in days divide pond 1 time, and the 25th age in days divides pond 1 time again, make fry density reduce half at every turn; Bait feeding method is 2-4 age in days, and open-mouthed bait-SS type of throwing something and feeding freshwater rotifer sieves with 100 bolting silks, maintains density 7-9/ml, and supplement 3 every day; 5-7 age in days, the small-sized freshwater rotifer of throwing something and feeding, bait 100 bolting silks sieve, and maintain density 7-9/ml, and supplement 3 every day; 8-11 age in days, the medium-sized freshwater rotifer of throwing something and feeding, bait 80 bolting silks sieve, and maintain density 5-7/ml, and supplement 2 every day; 12-14 age in days, the large-scale freshwater rotifer of throwing something and feeding, bait 60 bolting silks sieve, and maintain density 5-7/ml, and supplement 2 every day, supplements the fairy shrimp young, throws something and feeds every day 2 times, and feeding volume is depending on residual bait amount in pond; 15-18 age in days, the large-scale freshwater rotifer of throwing something and feeding, small-sized cladocera and copepoda nauplius, bait 40 bolting silks sieve, and maintain density 3-5/ml, and supplement 2 every day, increases the feeding volume of the fairy shrimp young, throws something and feeds every day 2 times; 19-25 age in days, throw something and feed medium-sized cladocera and copepoda nauplius, bait 30 bolting silks sieve, the density of throwing something and feeding 3-4/ml, supplement 2 every day, has a small amount of surplus material to be as the criterion when throwing something and feeding for the second time, reduces the feeding volume of the fairy shrimp young, throws something and feeds every day 1 time; 26-30 age in days, throw something and feed large-scale cladocera, copepoda, bait 20 bolting silks sieve, the density of throwing something and feeding 4-6/ml, supplement 2 every day, has a small amount of surplus material to be as the criterion when throwing something and feeding for the second time, and fry total length 0.9-1.8cm goes out pond and puts pond in a suitable place to breed.
The present invention with respect to the outstanding feature of prior art is: 1) created a set of technology of utilizing small-sized fertilized egg to cultivate fry, effectively utilized the small-sized fertilized egg of fugu obscurus, laid a good foundation for reducing parent's deposit; 2) improved the seed rearing survival rate that the small-sized incubating oosperm of fugu obscurus newly hatched larvae is out cultivated the black prelarva seedling of total length 1cm, survival rate is 75-85%.The present invention has saved production cost, and easy and simple to handle, is easy to be accepted and promote.
Embodiment
The specific embodiment of the present invention by incubating oosperm, put seedling, inflation, water temperature control, water quality control, point pond and bait feeding integrated cultural technique and form, below set forth one by one every concrete technology:
1, incubating oosperm: the fertilized egg obtaining according to the conventional artificial fecundation method of fugu obscurus, the ovum that every tail parent population produces is hatching separately, the ovum individual difference that general same tail parent population produces is few, note observing simultaneously, small-sized fertilized egg is concentrated in several hatcheries, and hatchery is cement pit, and suitable for reading is rectangle (long 125cm × wide 85cm × dark 40cm), end opening is taper (long 125cm × wide 85cm × dark 40cm), and volume is 0.6m
3, practical water body 0.5m
3.Adopt hydrostatic inflation hatching, inflation makes Chi Shuicheng boiling shape, and water temperature is 21-22 ℃, and triploidy rate is controlled at ten thousand/m of 8-10
3; Intensity of illumination is advisable with 800-1000lx, the bilayer film shading of sheltering from heat or light on daytime, the light filling of turning on light evening; Change water every day 1 time, each 4/5.Through about 100 hours, start rupture of membranes, then through 24 hours, more than 98% normotrophic embryo rupture of membranes.
2, put seedling: newly hatched larvae is supported temporarily after 2 days in hatchery, moves into seed rearing pond (10 m
3-20m
3), put seedling density: 1.0 ten thousand tails/m
3, putting seedling water level is 40cm, the water temperature in hatchery can not exceed 0.2 ℃ with the temperature difference of putting in a suitable place to breed in object pool.
3, inflation: while putting seedling, the density of gas exhausting stone is 1.5/m
2, reduce to 1/m to later stage density
2.
4, water temperature control: while putting seedling, water temperature control is 21.5 ± 0.2 ℃ heated up 1.0 ℃ every 24 hours later, rose to 24.5 ± 0.5 ℃ of water temperatures.
5, water quality control: while putting seedling, the depth of water is 400mm, starts progressively to add water until fill it up with on the 2nd day, within the 4th day, starts to inhale bottom blowdown and changes water, inhales 1 time, the end later every day, changes water 2/3 every other day.
6, Fen Chi: generally divide pond 2 times, make fry density reduce half at every turn, the time is respectively and puts seedling the 18th age in days, the 25th age in days left and right.
7, bait adopts the biological feed that cultivate in conventional organic loam pond, and feeding method is as follows:
2-4 age in days: in the opening stage, prelarva hatching is after 60-72 hour, and yolk sac fades away, fish body level is moved about, and now fry has started to ingest, therefore the open-mouthed bait-SS type freshwater rotifer of will throwing something and feeding is in time as cornicult Brachionus, 100 bolting silks sieve, and maintain density 7-9/ml, and supplement 3 every day.
5-7 age in days: bait adopts 200 order collections, 100 bolting silks to sieve, and is mainly small-sized freshwater rotifer, as: cornicult Brachionus, Brachionus calyciflorus, maintain density 7-9/ml, supplement 3 every day.
8-11 age in days: bait adopts 150 order collections, 80 bolting silks to sieve, and is mainly medium-sized freshwater rotifer, maintains density 5-7/ml, and supplement 2 every day.
12-14 age in days: bait adopts 150 order collections, 60 bolting silks to sieve, and is mainly large-scale freshwater rotifer, maintains density 5-7/ml, and supplement 2 every day; The appropriate supplementary fairy shrimp young, throws something and feeds 2 every day, and feeding volume is depending on residual bait amount in pond.
15-18 age in days: bait adopts 80 order collections, 40 bolting silks to sieve, and is mainly large-scale freshwater rotifer, small-sized cladocera and copepoda nauplius, maintains density 3-5/ml, and supplement 2 every day; Increase the feeding volume of the fairy shrimp young, throw something and feed every day 2 times.
19-25 age in days: bait adopts 80 order collections, 30 bolting silks to sieve, is mainly medium-sized cladocera and copepoda nauplius, the density of throwing something and feeding 3-4/ml, and supplement 2 every day, has a small amount of surplus material to be as the criterion when throwing something and feeding for the second time; Reduce the feeding volume of the fairy shrimp young, throw something and feed every day 1 time.
26-30 age in days: bait adopts 80 order collections, 20 bolting silks to sieve, and is mainly large-scale cladocera, copepoda, as Moina, carina Magna, copepoda adult, the density of throwing something and feeding 4-6/ml, supplement 2 every day, has a small amount of surplus material to be as the criterion when throwing something and feeding for the second time.Fry total length 0.9-1.8cm, can go out pond and put pond in a suitable place to breed.
Above-mentioned embodiment, through in the Technique of Raising Seeedling centre base test of this institute, obtains the small-sized fertilized egg of fugu obscurus, obtains newly hatched larvae 200,000 tails after hatching, moves into a 20m
3seed rearing pond, the nurturing period extends the Feeding time of wheel animalcule, 2 times, point pond obtains the black prelarva seedling 160,000 tails left and right of total length 1.2cm, seed rearing survival rate 80% through the cultivations of 28 days.
Claims (1)
1. the method that the small-sized fertilized egg of fugu obscurus is cultivated fry, by incubating oosperm, put seedling, inflation, water temperature control, water quality control, point pond and bait feeding cultivating technique and form, it is characterized in that incubating oosperm is that small-sized fertilized egg is put into hatchery, triploidy rate is controlled at ten thousand/m of 8-10
3, hatchery is practical water body 0.5m
3conical bottom cement pit, adopt hydrostatic inflation to make Chi Shuicheng boiling shape, water temperature is 21-22 ℃, intensity of illumination 800-1000lx, daytime is with the bilayer film shading of sheltering from heat or light, the light filling of turning on light evening, change water every day 1 time, change water 4/5 at every turn, start rupture of membranes through 100 hours, start after rupture of membranes through within 24 hours, there being more than 98% normotrophic embryo's rupture of membranes; Putting seedling is that newly hatched larvae is supported temporarily after 2 days and moved into seed rearing pond in hatchery, and putting seedling density is 1.0 ten thousand tails/m
3putting seedling water level is 40cm, the 2nd day starts to add water until fill it up with, within the 4th day, start to inhale bottom blowdown and change water, inhale 1 time, the end later every day, change water 2/3 every other day, the temperature difference in water temperature and the hatchery in seed rearing pond can not exceed 0.2 ℃, heats up 1.0 ℃ every 24 hours later, rise to 24.5 ± 0.5 ℃ of water temperatures, while putting seedling, the density of gas exhausting stone is 1.5/m
2, reduce to gradually 1/m of density
2; Newly hatched larvae to the 18 ages in days divide pond 1 time, and the 25th age in days divides pond 1 time again, make fry density reduce half at every turn; Bait feeding method is 2-4 age in days open-mouthed bait-SS type freshwater rotifer of throwing something and feeding, and sieves with 100 bolting silks, maintains density 7-9/ml, and supplement 3 every day; The 5-7 age in days small-sized freshwater rotifer of throwing something and feeding, bait 100 bolting silks sieve, and maintain density 7-9/ml, and supplement 3 every day; The 8-11 age in days medium-sized freshwater rotifer of throwing something and feeding, bait 80 bolting silks sieve, and maintain density 5-7/ml, and supplement 2 every day; The 12-14 age in days large-scale freshwater rotifer of throwing something and feeding, bait 60 bolting silks sieve, and maintain density 5-7/ml, and supplement 2 every day, supplements the fairy shrimp young, throws something and feeds every day 2 times, and feeding volume is depending on residual bait amount in pond; 15-18 age in days throw something and feed large-scale freshwater rotifer, small-sized cladocera and copepoda nauplius, bait 40 bolting silks sieve, and maintain density 3-5/ml, and supplement 2 every day, increases the feeding volume of the fairy shrimp young, throws something and feeds every day 2 times; 19-25 age in days throw something and feed medium-sized cladocera and copepoda nauplius, bait 30 bolting silks sieve, and the density of throwing something and feeding 3-4/ml, supplement 2 every day, has a small amount of surplus material to be as the criterion when throwing something and feeding for the second time, reduces the feeding volume of the fairy shrimp young, throws something and feeds every day 1 time; 26-30 age in days throw something and feed large-scale cladocera, copepoda, bait 20 bolting silks sieve, and the density of throwing something and feeding 4-6/ml, supplement 2 every day, has a small amount of surplus material to be as the criterion when throwing something and feeding for the second time, and fry total length 0.9-1.8cm goes out pond and puts pond in a suitable place to breed.
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110771542A (en) * | 2019-12-05 | 2020-02-11 | 南京坤泰农业发展有限公司 | Breeding method for improving fry hatchability |
CN112772470A (en) * | 2020-12-31 | 2021-05-11 | 中国水产科学研究院黑龙江水产研究所 | Artificial cultivation method of juvenile glyptosternum maculatum |
CN114027229A (en) * | 2021-11-30 | 2022-02-11 | 中国水产科学研究院黄海水产研究所 | Novel method for cultivating takifugu rubripes fries |
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CN101248770A (en) * | 2008-03-28 | 2008-08-27 | 河北省水产研究所 | Method for shortening cultivation period of Fugu flavidus |
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110771542A (en) * | 2019-12-05 | 2020-02-11 | 南京坤泰农业发展有限公司 | Breeding method for improving fry hatchability |
CN112772470A (en) * | 2020-12-31 | 2021-05-11 | 中国水产科学研究院黑龙江水产研究所 | Artificial cultivation method of juvenile glyptosternum maculatum |
CN114027229A (en) * | 2021-11-30 | 2022-02-11 | 中国水产科学研究院黄海水产研究所 | Novel method for cultivating takifugu rubripes fries |
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