CN113287548B - Artificial propagation method for yellow-shelled fishes - Google Patents
Artificial propagation method for yellow-shelled fishes Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/13—Prevention or treatment of fish diseases
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
- A01K61/17—Hatching, e.g. incubators
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses an artificial propagation method for yellow-shelled fishes, and relates to the field of fish culture. The artificial propagation method for the yellow-shelled fishes comprises seven steps of parent fish selection, parent fish feeding, spawning induction and hatching workshop building, artificial spawning induction, artificial fertilization, artificial hatching and seedling management. The artificial propagation method for the yellow-shelled fishes is characterized in that wheat and barley malt are fed to increase the development degree of gonads of the yellow-shelled fishes, the reproductive capacity of the yellow-shelled fishes is improved, the parent fishes are covered by a black net after injection of an oxytocic drug, the negative influence of external light on the parent fishes is relieved by scouring stimulation of water, the hormone can be stimulated to fully exert the effect in the parent fishes, the oxytocic effect is improved, the gas temperature and the water temperature are accurately controlled during the oxytocic, the strict requirement on the temperature during the yellow-shelled fishes propagation production is met, the precise control on the needle insertion depth and other details of the injected oxytocic drug is also achieved, and the method is scientific, efficient, practical, simple to operate and beneficial to popularization and use.
Description
Technical Field
The invention relates to the technical field of fish culture, in particular to an artificial propagation method for yellow-shell fish.
Background
Huang Ke fish, a dietetic fish in Mianmi border, named as four-whisker barbel, is a specific river wild native fish variety in the Anhui river estuary river basin of Zhengkang county in Lincang city, yunnan province, and has beautiful appearance and high ornamental value; tenderness, good edible mouthfeel, rich nutrition and delicious taste, and is an excellent tonic; the common eating of the yellow-shelled fish can achieve the effects of dispelling wind and removing dampness, and has medicinal function; meanwhile, the product has beautiful appearance, good quality, medicinal function and other genetic characteristics, and has high breeding value. Therefore, more and more farmers start to artificially breed the yellow-shelled fishes in recent years, but the requirements of the yellow-shelled fishes on water quality and temperature are higher, the wild yellow-shelled fishes are fished from the field for breeding in the existing breeding method, the yellow-shelled fishes cannot be naturally bred in the breeding pond due to the influence of various natural condition factors, the breeding survival rate of the fished wild yellow-shelled fishes is lower, the large-scale breeding is difficult, the economic benefit cannot be improved, and the dosage used for hastening spawning of the conventional fishes cannot hasten the spawning of the yellow-shelled fishes.
Disclosure of Invention
Technical problem to be solved
Aiming at the genetic characteristic that the yellow-shell fish can not be bred in the culture pond, the yellow-shell fish eggs can not be induced by adopting the dosage used by the conventional fish induced spawning. The invention provides an artificial propagation method for yellow-shelled fishes, which solves the problems that most farmers need to catch wild yellow-shelled fishes from the field for culture, the culture survival rate is low, and the large-scale culture is difficult.
(II) technical scheme
In order to achieve the purpose, the invention provides the following technical scheme: an artificial propagation method for yellow-shelled carps, which comprises the following steps:
1. selecting parent fishes;
selecting 300 female fishes which are artificially domesticated, have strong physique and good development every 2 months, each fish is 2-6 jin, and the age of the fish is more than 4 years old; 900 male fishes, each fish is 0.5 to 2 jin, the age of the fishes is more than 1 year old, the fishes are placed into a parent fish culture pond for culture, and the area of the culture pond is 1 to 3 mu;
2. feeding parent fish;
wheat and barley malt are mainly thrown and fed, various vegetables, fresh fishes and shrimps are fed in a matched mode to increase plant and animal protein, artificial compound feed is not fed, the feeding frequency reaches 3 to 5 times, and the feeding time is as follows: feeding is carried out 7 to 8 am, 11 to 12 am, 15 to 16 pm and 19 to 20 pm, the feeding amount is 8 and full, and the feeding time is one month;
3. establishing an induced spawning and hatching workshop;
1) One workshop with the area of 400 square meters;
2) Spawning ponds (cylinders) of 1 to 3 per 25 cubic meters;
3) One (cube) 50 cubic meters of settling pond;
4) One (cube) 50 cubic meters of disinfection tank;
4. artificial hastening parturition;
1) An induced spawning device: a disposable syringe with 5 ml to 10 ml, normal saline, an egg receiving appliance, an appliance for fixing parent fish, a temporary fish tank and the like;
2) An oxytocic drug: chorionic Gonadotropin (HCG), domperidone, and luteinizing hormone-releasing hormone analog (LRH-a), etc.;
3) The operator: more than 3 persons;
4) Effect time: the effect time is 16-18 hours after one-time injection; two needles are separately injected, the effect time after the first needle injection is 14-15 hours, and the effect time after the second needle injection is 12-13 hours;
5) The specific operation method comprises the following steps: catching the parent fish in the parent fish culture pond, observing and touching the fish body by naked eyes, selecting artificially domesticated female fish and male fish with strong physique, and putting the female fish and the male fish with good gonad development into a temporary fish tank according to the proportion of 1:3; registering the current time, measuring the current air temperature and water temperature, temporarily taking out the female fish and the male fish from the fish tank, fixing the female fish and the male fish with a device for fixing the parent fish, respectively inserting needles 45 degrees below pectoral fins of the female fish and the male fish, injecting an oxytocic into the thoracic cavity, mastering the needle insertion depth during injection, wherein the needle insertion depth does not work enough and hormone does not work enough, and the needle insertion depth can prick the heart of the parent fish to cause death of the parent fish; after the injection of the parent fish, putting the parent fish into a spawning pond, covering the spawning pond by using a black net, performing scouring stimulation by using clear micro-flow water, performing artificial spawning induction, and respectively catching the parent fish for artificial fertilization when the effect time is up;
5. artificial fertilization;
1) Artificial insemination apparatus: goose feather, egg-receiving utensil, dry towel, disinfectant (salt, potassium permanganate solution, povidone iodine solution), temporary fish tank, etc.;
2) The operator: more than 3 persons;
3) The specific operation method comprises the following steps: one person controls the parent fish and seals the cloaca of the parent fish by hands at the same time, one person uses a dry towel to wipe water drops on the abdomen of the parent fish and squeeze eggs and sperms on the abdomen of the parent fish, the other person uses an egg receiving device to quickly receive the eggs and the sperms, the fertilization of the eggs and the sperms cannot exceed 1 minute, meanwhile, goose feather is used for fully stirring the eggs and the sperms anticlockwise for 2-3 minutes to ensure the full fertilization, then a proper amount of clear water is added for stirring for 2-3 minutes, and the mixture is placed into hatching equipment for hatching;
6. artificial incubation;
1) An incubation device: 2 to 3 oxygenation pumps of 350W, 20 to 50 meters of oxygenation pipes, 20 to 50 meters of water pipes, 2 to 4 big barrels of 2 x 2 meters in diameter, 5 to 10 meters of 40-mesh spun silk, 5 to 10 vegetable washing baskets of 0.5 x 1 meter x 0.3 meter, one roll of fishing line, 4 bottles of physiological saline, a plurality of plastic foams, 2 to 5 thermostats of thermostats, 5 to 10 injectors of 10 to 20 milliliters, and an incubation water tank;
2) The operator: more than 3 persons;
3) Demoulding: the demoulding time is 4 to 6 days when the water temperature is between 20 and 22 ℃; the demoulding time is 3 to 4 days when the water temperature is 24 to 25 ℃; the demoulding time is 3 days when the water temperature is between 26 and 28 ℃;
4) The specific method of operation: one person puts a self-made hatching floating plate into a hatching water tank, an oxygen increasing device is placed, one person puts fertilized eggs into the floating plate to be uniformly distributed, one person uses 3% saline solution to disinfect water in the hatching tank, goose down is used for uniformly stirring the fertilized eggs every 2 to 3 hours, the water temperature is kept constant, the dissolved oxygen is moderate, the fertilized eggs need 48 hours to grow stably, and after 48 hours, the fish fry is waited to be stripped;
7. seedling stage management;
1) A management method of demoulding to opening;
controlling the water temperature, keeping the water temperature consistent with the water temperature during incubation, observing whether the oxygen increasing machine works normally, and feeding the opening material of the fry;
2) A management method of the water bloom seedling stage;
a. controlling the water temperature and observing whether the aerator works normally or not, measuring the water quality every three days, and keeping the water flower seedling culture box clean;
b. feeding: feeding for 4 to 5 times every day, wherein the bait coefficient is 2 to 3 percent, and the purpose of feeding a plurality of meals less is achieved;
c. selecting baits: selecting natural daphnia, soybean milk, salted duck eggs and the like which have protein content of more than 45% and are caught in Tongwei Kouye or a pond;
d. changing water in the seedling pool: once Miao Xiangshui changed for 5 to 7 days;
e. and (3) disinfection: disinfecting the pond water and the fish fries once by using a proper amount of potassium permanganate or povidone iodine solution for 5 to 7 days;
f. the feeding method comprises the following steps: follow the four definite principles;
J. disease control: and (4) using a proper amount of the clean fish and insect crystals to comprehensively sprinkle 2000 times of pond water. 2 times per month, 3-5 days each time, 1-2 days between medication; the florfenicol powder is added and fed for 2 times per month, and each time lasts for 3 to 5 days.
3) Managing method of cun seedling stage;
a. controlling the water temperature and observing whether the aerator works normally, measuring the water quality once every 7 days, and keeping the cun-seedling culture pond clean;
b. feeding: feeding for 4 to 5 times every day, wherein the bait coefficient is 3 to 5 percent, and the purpose of feeding a plurality of meals less is achieved;
a. selecting bait: selecting puffing materials or various vegetable scraps with the calibers of Tongwei 1.0 mm and the protein content of which is more than 32-36 percent;
c. changing water in the seedling pool: replacing the pool water once in 5 to 7 days;
d. and (3) disinfection: disinfecting the pond water and the fry once by using a proper amount of potassium permanganate or povidone iodine solution in 5 to 7 days;
e. the feeding method comprises the following steps: follow the four definite principles;
F. disease control: and (3) using a proper amount of the clean fish and insect crystals to fully sprinkle 2000 times of pond water to the seedling pond. 2 times per month, 3-5 days each time, 1-2 days between medication; the florfenicol powder is added and fed for 2 times per month, and each time lasts for 3 to 5 days.
Preferably, in the step of artificial induced spawning, the injection of the induced spawning medicament dose: injecting one injection once, injecting 7 mg and 7 mg of a mixture of oxytocin domperidone and luteinizing hormone releasing hormone analogue (LRH-A) into each kilogram of female fish, mixing 1-2 ml of normal saline and hormone into each female and male fish, and if the male fish is small, properly reducing the dosage of the normal saline; two needles are separately injected, the first needle is used for injecting 2 mg and 2 microgram of a mixture of oxytocin domperidone and luteinizing hormone releasing hormone analogue (LRH-A) per kilogram of female fish, each female and male parent fish is mixed with hormone by 1-2 ml of normal saline, and if the male fish is small, the dosage of the normal saline can be properly reduced; and injecting 6mg and 6mg of a mixture of oxytocin domperidone and luteinizing hormone releasing hormone analogue (LRH-A) into each kilogram of female fish, mixing 1-2 ml of normal saline and hormone into each female and male parent fish, and reducing the dosage of the normal saline by a proper amount if the male fish is small.
Preferably, in the artificial induced spawning step, the number of the male fish should be reduced by half when the induced spawning medicine is injected.
Preferably, in the step of artificial induced spawning, the air temperature is controlled to be 22-32 ℃, and the water temperature is controlled to be 20-28 ℃.
Preferably, in the artificial fertilization step, the room temperature is controlled to be 22-25 ℃, and the water temperature is controlled to be 24-25 ℃.
Preferably, in the artificial incubation step, the room temperature is controlled to be 22-25 ℃, the water temperature is controlled to be 24-25 ℃, and the oxygen dissolution amount is controlled to be 6 mg/above.
Preferably, in the artificial incubation step, the water in the incubation water tank is changed every day, and fertilized eggs which do not develop are selected every day.
Preferably, in the management step of the seedling stage, the fry is fragile after being stripped, the fry moves from the bottom of the hatching net cage to the upper part or down, the fry cannot be eaten, the fry is only required to be kept at constant temperature, oxygenated and cleaned, and the fry opening material is fed after the fry is jacked to the waist and can be horizontally moved.
(III) advantageous effects
The invention provides an artificial propagation method for yellow-shelled carps, which has the following beneficial effects that the yellow-shelled carps cannot be naturally propagated in a culture pond due to the special genetic characteristics of the yellow-shelled carps:
1. the artificial propagation method for the yellow-shelled fishes is characterized in that the yellow-shelled fishes with excellent quality, strong physique and good gonad development are selected as parent fishes, the quality of the propagated yellow-shelled fishes is guaranteed, meanwhile, wheat and barley malt are fed to increase the development degree of the gonads of the yellow-shelled fishes, the reproductive capacity of the yellow-shelled fishes is improved, the parent fishes are covered by a black net after the parent fishes are injected with an oxytocic, the negative influence of external light on the parent fishes is relieved by scouring stimulation of added water, protection and guarantee are provided, hormone can be stimulated to fully exert the effect in the parent fishes, the oxytocic effect is improved, the gas temperature and the water temperature are accurately controlled during the oxytocic, the severe requirements on the temperature during the yellow-shelled fish propagation production are met, the accurate control on the depth of the injection needle depth and other details of the oxytocic medicine is also achieved, the method is scientific, efficient, practical, simple to operate and beneficial to popularization and use, and compared with the artificial propagation method for the ordinary conventional fishes, the method for hastening the spawning of the yellow-shelled fishes greatly solves the problem that the spawning rate of the yellow-shelled fishes during the artificial propagation of the yellow-shelled fishes.
2. The artificial propagation method for the yellow-shelled fishes is simple and efficient in parent fish egg and sperm collection operation, rapid in operation and short in interval time, effectively solves the problem of quality reduction of the eggs and the sperms caused by overlong time, meanwhile, the goose feather is used for stirring and mixing the water-added sperms and the eggs to fully mix the two, guarantee is provided for fertility rate, equipment is disinfected regularly and comprehensively during hatching of fertilized eggs, pollution of bacteria to the fertilized eggs is reduced, air temperature and water temperature are accurately controlled to provide sufficient oxygen for hatching of the fertilized eggs, the water temperature is kept consistent with the water temperature during hatching after hatching, water bloom fry death caused by changes of external temperature is avoided, natural water fleas, soybean milk, salted duck eggs and the like are used as feeds of the water bloom fishes, sufficient protein is provided for the yellow-shelled fishes in the water bloom stage, the adaptive temperature and the feeds for the yellow-shelled fishes in the cunning stage are also given, and compared with the artificial propagation method for common conventional fishes, the method greatly improves the early stage of the yellow-shelled fishes, reduces loss and improves economic benefits.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be obtained by a person skilled in the art without making any creative effort based on the embodiments in the present invention, belong to the protection scope of the present invention.
The first embodiment is as follows:
an artificial propagation method for yellow-shelled fishes, comprising the following steps:
1. selecting parent fishes;
selecting 300 female fishes which are strong in physique and good in development and are bred manually in 2 months every year, wherein each female fish is 2 jin and the age of the female fish is more than 4 years old; 900 male fishes, each of which is 0.5 jin and the age of which is more than 1 year, are placed into a parent fish breeding pond for breeding, and the area of the breeding pond is 1 mu;
2. feeding parent fish;
wheat and barley malt are mainly thrown and fed, various vegetables, fresh fishes and shrimps are fed in a matched mode to increase plant and animal protein, artificial compound feed is not fed, the feeding frequency reaches 3 times, and the feeding time is as follows: feeding is carried out at 7 to 8 am, 11 to 12 am, 15 to 16 pm and 19 to 20 pm, the feeding amount is 8 and the feeding time is one month;
3. establishing an induced spawning and hatching workshop;
1) One workshop with the area of 400 square meters;
2) The number of spawning ponds (cylinders) is 1 per 25 cubic meters;
3) One (cube) 50 cubic meters of settling pond;
4) One (cube) 50 cubic meters of disinfection tank;
4. artificial hastening parturition;
1) An induced spawning device: a disposable 5 ml syringe, normal saline, an egg receiving device, a device for fixing parent fish, a temporary fish tank and the like;
2) An oxytocic drug: chorionic Gonadotropin (HCG), domperidone, and luteinizing hormone-releasing hormone analog (LRH-a), etc.;
3) The operator: more than 3 persons;
4) Air temperature: 22 ℃;
5) Water temperature: 20 ℃;
6) Water quality: close to the national drinking water standard.
7) The dosage of the medicine is as follows: injecting one injection once, wherein 7 mg and 7 mg of a mixture of oxytocin domperidone and luteinizing hormone releasing hormone analogue (LRH-A) are injected into each kilogram of female fish, and each female fish and each male fish are mixed with hormone by using 1 ml of normal saline; two needles are separately injected, the first needle is used for injecting 2 mg and 2 microgram of a mixture of oxytocin domperidone and luteinizing hormone releasing hormone analogue (LRH-A) per kilogram of female fish, and each female and male parent fish is mixed with hormone by 1 milliliter of normal saline; secondly, injecting 6mg and 6 microgram of a mixture of oxytocin domperidone and luteinizing hormone releasing hormone analogue (LRH-A) into each kilogram of female fish, and mixing each female and male parent fish with 1 ml of normal saline and the hormone;
8) Effect time: the effect time after one-time injection is 16 hours; two needles are separately injected, the effect time after the first needle injection is 14 hours, and the effect time after the second needle injection is 12 hours;
9) The specific operation method comprises the following steps: catching the parent fish in the parent fish culture pond, and selecting the female fish and the male fish with strong physique and good gonad development by observing and touching the fish body with naked eyes, and putting the female fish and the male fish into a temporary fish tank according to the proportion of 1:3; registering the current time, measuring the current air temperature and water temperature, temporarily taking out the female fish and the male fish from the fish tank, fixing the female fish and the male fish with a device for fixing the parent fish, respectively inserting needles at 45 degrees below pectoral fins of the female fish and the male fish, injecting an oxytocic into the chest cavity, halving the male fish when injecting the oxytocic, controlling the needle insertion depth during injection, leading the needle insertion depth not to work as long as hormone does not work, and leading the needle insertion too deep to prick the heart of the parent fish to cause death of the parent fish; after the injection of the parent fish, putting the parent fish into a spawning pond, covering the spawning pond by using a black net, performing scouring stimulation by using clear micro-flowing water, performing artificial spawning induction, and respectively catching the parent fish for artificial fertilization when the effect time is up;
5. artificial fertilization;
1) Artificial insemination apparatus: goose feather, egg-receiving utensil, dry towel, disinfectant (salt, potassium permanganate solution, povidone iodine solution), temporary fish tank, etc.;
2) The operator: more than 3 persons;
3) Room temperature: 22 ℃;
4) Water temperature: 24 ℃;
5) Water quality: close to the national drinking water standard.
6) The specific operation method comprises the following steps: one person controls the parent fish and seals the cloaca of the parent fish by hands at the same time, one person wipes water drops on the abdomen of the parent fish by a dry towel and extrudes ova and sperms on the abdomen of the parent fish at the same time, the other person uses an ovum receiving device to quickly receive the ova and the sperms, the fertilization of the ova and the sperms cannot exceed 1 minute, meanwhile, goose feather is used for fully stirring the ova and the sperms anticlockwise for 2 minutes to ensure the full fertilization, then proper amount of clear water is added for stirring for 2 minutes again, and the mixture is placed into hatching equipment for hatching;
6. artificial incubation;
1) An incubation device: 2 oxygenation pumps of 350W, 20 oxygenation pipes, 20 water pipes, 2 vats of 2 with the diameter of 2 multiplied by 2 meters, 5 gauze of 40 meshes, 5 vegetable washing baskets of 0.5 multiplied by 1 multiplied by 0.3 meters, one reel of fishing line, 4 bottles of physiological saline, a plurality of plastic foams, 2 thermostats, 5 injectors of 10 milliliters and an incubation water tank;
2) The operator: more than 3 persons;
3) Room temperature: 22 ℃;
4) Water temperature: 24 ℃;
5) Water quality: is close to the national standard drinking water standard.
6) Oxygen dissolved amount: 6mg/L or more;
7) Demoulding: the demoulding time is 4 days when the water temperature is 20 ℃; the demoulding time is 3 days when the water temperature is 24 ℃; the demoulding time is 3 days when the water temperature is 26 ℃;
8) The specific method of operation: one person puts the self-made hatching floating plate into a hatching water tank, places an oxygen increasing device, one person puts the fertilized eggs into the floating plate to be uniformly distributed, one person uses 3% saline solution to disinfect the water in the hatching tank, goose feathers are used for uniformly stirring the fertilized eggs every 2 hours, the water temperature is kept constant, the dissolved oxygen is moderate, the water in the hatching water tank needs to be changed every day, the fertilized eggs which do not develop are selected every day, the fertilized eggs need to develop stably within 48 hours, and after 48 hours, the fish fry is waited to be demoulded;
7. managing in a seedling stage;
1) A management method of demoulding to opening;
controlling the water temperature, keeping the water temperature consistent with the water temperature during incubation, observing whether the oxygen-increasing machine works normally or not, ensuring that the fry is fragile after the fry is demoulded, enabling the fry to move in a mode of moving from the bottom of an incubation net cage to the upper part or the lower part, ensuring that the fry cannot be eaten, only paying attention to constant temperature, increasing oxygen and cleaning the incubation net cage, and starting feeding fry opening materials after the fry is jacked and can move horizontally;
2) A management method of the water bloom seedling stage;
a. controlling the water temperature and observing whether the aerator works normally, measuring the water quality every three days, and keeping the water flower seedling culture box clean;
b. feeding: feeding for 4 times every day, wherein the bait coefficient is 2 percent, and the purpose of feeding a plurality of meals less is achieved;
c. selecting bait: selecting natural daphnia, soybean milk, salted duck eggs and the like which have protein content of more than 45% and are caught in Tongwei Kouye or a pond;
d. changing water in the seedling pool: once in 5 days, miao Xiangshui is replaced;
e. and (3) disinfection: disinfecting the pond water and the fry once by using a proper amount of potassium permanganate or povidone iodine solution after 5 days;
f. disease control: and (4) using a proper amount of the clean fish and insect crystals to comprehensively sprinkle 2000 times of pond water. 2 times per month, 3-5 days each time, 1-2 days between medication; the florfenicol powder is added and fed for 2 times per month, and each time lasts for 3 to 5 days.
g. The feeding method comprises the following steps: follow the four definite principles;
3) Managing method of cun seedling stage;
a. controlling the water temperature and observing whether the aerator works normally, measuring the water quality once every 7 days, and keeping the cun-seedling culture pond clean;
b. feeding: feeding for 4 to 5 times every day, wherein the bait coefficient is 3 percent, and the purpose of feeding a plurality of meals less is achieved;
a. selecting bait: selecting puffing materials or various vegetable scraps with the calibers of 1.0 mm of Tongwei with the protein content of more than 32 percent;
c. changing water in the seedling pool: replacing the pool water once in 5 days;
d. and (3) disinfection: disinfecting the pond water and the fry once by using a proper amount of potassium permanganate or povidone iodine solution after 5 days;
e. disease control: and (4) using a proper amount of the clean fish and insect crystals to comprehensively sprinkle 2000 times of pond water. 2 times per month, 3-5 days each time, 1-2 days between medication; the florfenicol powder is added and fed for 2 times per month, and each time lasts for 3 to 5 days.
f. The feeding method comprises the following steps: follow the four rules.
Example two:
an artificial propagation method for yellow-shelled fishes, comprising the following steps:
2. selecting parent fishes;
selecting 300 female fishes which are strong in physique and good in development and are bred manually in 2 months every year, wherein each female fish is 4 jin and the age of the female fish is more than 4 years old; 900 male fishes, each of which is 1 jin and the age of which is more than 1 year, are placed into a parent fish breeding pond for breeding, and the area of the breeding pond is 2 mu;
2. feeding parent fish;
wheat and barley malt are mainly thrown to feed, various vegetables, fresh fishes and shrimps are thrown to feed in a matching mode to increase plant and animal protein, artificial compound feed is not fed, the number of times of throwing to feed reaches 4 times, and the time of throwing to feed is: feeding is carried out at 7 to 8 am, 11 to 12 am, 15 to 16 pm and 19 to 20 pm, the feeding amount is 8 full, and the feeding days are one month;
3. establishing an induced spawning and hatching workshop;
1) One workshop with the area of 400 square meters;
2) 2 oxytocic ponds (cylinders) each of 25 cubic meters;
3) One (cube) 50 cubic meters of settling pond;
4) One (cube) 50 cubic meters of disinfection tank;
4. artificial hasten parturition;
1) An induced spawning device: a disposable 5 ml syringe, normal saline, an egg receiving device, a device for fixing parent fish, a temporary fish tank and the like;
2) An oxytocic drug: chorionic Gonadotropin (HCG), domperidone, and luteinizing hormone-releasing hormone analog (LRH-a), etc.;
3) The operator: more than 3 persons;
4) Air temperature: 27 ℃;
5) Water temperature: 24 ℃;
6) Water quality: close to the national drinking water standard.
7) The dosage of the medicine is as follows: injecting one injection once, injecting 7 mg and 7 microgram of a mixture of oxytocin domperidone and luteinizing hormone releasing hormone analogue (LRH-A) into each kilogram of female fish, mixing 1.5 ml of normal saline and hormone into each female and male fish, and if the male fish is small, reducing the dosage of the normal saline; two needles are separately injected, the first needle is used for injecting 2 mg and 2 microgram of a mixture of oxytocin domperidone and luteinizing hormone releasing hormone analogue (LRH-A) per kilogram of female fish, each female and male parent fish is mixed with hormone by 1.5 ml of normal saline, and if the male fish is small, the dosage of the normal saline can be properly reduced; secondly, injecting 6mg and 6mg of a mixture of oxytocin domperidone and luteinizing hormone releasing hormone analogue (LRH-A) into each kilogram of female fish, mixing 1.5 ml of normal saline and hormone into each female and male parent fish, and if the male fish is small, reducing the dosage of the normal saline in a proper amount;
8) Effect time: the effect time after one-time injection is 17 hours; two needles are separately injected, the effect time after the first needle injection is 14.5 hours, and the effect time after the second needle injection is 12.5 hours;
9) The specific operation method comprises the following steps: fishing out the parent fish in the parent fish culture pond, and selecting female fish and male fish with robust physique and good gonad development by observing and touching the fish body with naked eyes, and putting the female fish and the male fish into a temporary fish tank according to the proportion of 1:3; registering the current time, measuring the current air temperature and water temperature, temporarily taking out the female fish and the male fish from the fish tank, fixing the female fish and the male fish with a device for fixing the parent fish, respectively inserting needles at 45 degrees below pectoral fins of the female fish and the male fish, injecting an oxytocic into the chest cavity, halving the male fish when injecting the oxytocic, controlling the needle insertion depth during injection, leading the needle insertion depth not to work as long as hormone does not work, and leading the needle insertion too deep to prick the heart of the parent fish to cause death of the parent fish; after the injection of the parent fish, putting the parent fish into a spawning pond, covering the spawning pond by using a black net, performing scouring stimulation by using clear micro-flowing water, performing artificial spawning induction, and respectively catching the parent fish for artificial fertilization when the effect time is up;
5. artificial fertilization;
1) Artificial insemination apparatus: goose feather, egg-receiving utensil, dry towel, disinfectant (salt, potassium permanganate solution, povidone iodine solution), temporary fish tank, etc.;
2) The operator: more than 3 persons;
3) Room temperature: 24 ℃;
4) Water temperature: 25 ℃;
5) Water quality: close to the national drinking water standard.
6) The specific operation method comprises the following steps: one person controls the parent fish and seals the cloaca of the parent fish by hands at the same time, one person uses a dry towel to wipe water drops on the abdomen of the parent fish and squeeze eggs and sperms on the abdomen of the parent fish, the other person uses an egg receiving device to quickly receive the eggs and the sperms, the fertilization of the eggs and the sperms cannot exceed 1 minute, meanwhile, goose feathers are used for fully stirring the eggs and the sperms anticlockwise for 2.5 minutes to ensure the full fertilization, then a proper amount of clear water is added for stirring for 2.5 minutes again, and the mixture is placed into hatching equipment for hatching;
6. artificial incubation;
1) An incubation device: 3 oxygenation pumps of 350W, 35 oxygenation pipes, 35 water pipes, 3 vats of 2 multiplied by 2 meters in diameter, 8 spun silk of 40 meshes, 5 to 10 vegetable washing baskets of 0.5 multiplied by 1 meter multiplied by 0.3 meter, a reel of fishing line, 4 bottles of normal saline, a plurality of plastic foams, 5 thermostats, 7 injectors of 10 to 20 milliliters and an incubation water tank;
2) The operator: more than 3 persons;
3) Room temperature: 24 ℃;
4) Water temperature: 25 ℃;
5) Water quality: close to the national drinking water standard.
6) Oxygen dissolved amount: 6mg/L or more;
7) Demoulding: demoulding for 5 days when the water temperature is 21 ℃; the demoulding time is 4 days when the water temperature is 25 ℃; the demoulding time is 3 days when the water temperature is 27 ℃;
8) The specific method of operation: one person puts the self-made hatching floating plate into a hatching water tank, places an oxygen increasing device, one person puts the fertilized eggs into the floating plate to be uniformly distributed, one person uses 3% saline solution to disinfect the water in the hatching tank, goose feathers are used to uniformly stir the fertilized eggs every 2 to 3 hours, the water temperature is kept constant, the dissolved oxygen is moderate, the water in the hatching water tank needs to be changed every day, the fertilized eggs which do not develop are selected every day, the fertilized eggs need to develop stably within 48 hours, and the fish fry is waited to be demoulded after 48 hours;
7. seedling stage management;
1) A management method of demoulding to opening;
controlling the water temperature, keeping the water temperature consistent with the water temperature during incubation, observing whether the oxygen-increasing machine works normally or not, ensuring that the fry is fragile after the fry is demoulded, enabling the fry to move in a mode of moving from the bottom of an incubation net cage to the upper part or the lower part, ensuring that the fry cannot be eaten, only paying attention to constant temperature, increasing oxygen and cleaning the incubation net cage, and starting feeding fry opening materials after the fry is jacked and can move horizontally;
2) A management method of the water bloom seedling stage;
a. controlling the water temperature and observing whether the aerator works normally or not, measuring the water quality every three days, and keeping the water flower seedling culture box clean;
b. feeding: 5 times of feeding are carried out every day, the bait coefficient is 2.5 percent, and the purpose of feeding a plurality of meals less is achieved;
c. selecting bait: selecting natural daphnia, soybean milk, salted duck eggs and the like which have protein content of more than 45% and are caught in Tongwei Kouye or a pond;
d. changing water in the seedling pool: once in 6 days Miao Xiangshui;
e. and (3) disinfection: disinfecting the pond water and the fry once by using a proper amount of potassium permanganate or povidone iodine solution for 6 days;
f. disease control: and (3) using a proper amount of the clean fish and insect crystals to fully sprinkle 2000 times of pond water to the seedling pond. 2 times per month, 3-5 days each time, 1-2 days interval; the florfenicol powder is added and fed for 2 times every month for 3 to 5 days.
g. The feeding method comprises the following steps: follow the four definite principles;
3) Managing method of cun seedling stage;
a. controlling the water temperature and observing whether the aerator works normally, measuring the water quality once every 7 days, and keeping the cun-seedling culture pond clean;
b. feeding: feeding for 5 times every day, wherein the bait coefficient is 4 percent, and the purpose of feeding a plurality of meals less is achieved;
a. selecting bait: selecting puffing materials or various vegetable scraps with the protein content of more than 34% and the calibre of Tongwei 1.0 mm;
c. changing water in the seedling pool: replacing the pool water once in 6 days;
d. and (3) disinfection: disinfecting the pond water and the fry once by using a proper amount of potassium permanganate or povidone iodine solution for 6 days;
e. disease control: and (3) using a proper amount of the clean fish and insect crystals to fully sprinkle 2000 times of pond water to the seedling pond. 2 times per month for 3-5 days, with 1-2 days interval; the florfenicol powder is stirred and fed for 2 times per month, and each time lasts for 3 to 5 days.
f. The feeding method comprises the following steps: follow the four rules.
Example three:
an artificial propagation method for yellow-shelled carps, which comprises the following steps:
1. selecting parent fishes;
selecting 300 female fishes which are strong in physique and good in development and are bred manually in 2 months every year, wherein each female fish is 6 jin and the age of the female fish is more than 4 years old; placing 900 male fishes of which each one is 2 jin in age of more than 1 year old into a parent fish culture pond for culture, wherein the area of the culture pond is 3 mu;
2. feeding parent fish;
wheat and barley malt are mainly thrown and fed, various vegetables, fresh fishes and shrimps are fed in a matched mode to increase plant and animal protein, artificial compound feed is not fed, the feeding frequency reaches 5 times, and the feeding time is as follows: feeding is carried out 7 to 8 am, 11 to 12 am, 15 to 16 pm and 19 to 20 pm, the feeding amount is 8 and full, and the feeding time is one month;
3. establishing an induced spawning and hatching workshop;
1) One workshop with the area of 400 square meters;
2) The number of spawning ponds (cylinders) is 3 per 25 cubic meters;
3) One (cube) 50 cubic meters of settling pond;
4) One (cube) 50 cubic meters of disinfection tank;
4. artificial hasten parturition;
1) An induced spawning device: a disposable 5 ml syringe, normal saline, an egg receiving device, a device for fixing parent fish, a temporary fish tank and the like;
2) An oxytocic drug: chorionic Gonadotropin (HCG), domperidone, and luteinizing hormone-releasing hormone analog (LRH-a), etc.;
3) The operator: more than 3 persons;
4) Air temperature: at 32 ℃;
5) Water temperature: 28 deg.C
6) Water quality: close to the national drinking water standard.
7) The dosage of the medicine is as follows: injecting one injection once, injecting 7 mg and 7 microgram of a mixture of oxytocin domperidone and luteinizing hormone releasing hormone analogue (LRH-A) into each kilogram of female fish, mixing 2 ml of normal saline and hormone into each female and male fish, and if the male fish is small, reducing the dosage of the normal saline; two needles are separately injected, the first needle is used for injecting 2 mg and 2 microgram of a mixture of oxytocin domperidone and luteinizing hormone releasing hormone analogue (LRH-A) per kilogram of female fish, each female and male parent fish is mixed with 2 ml of normal saline and hormone, and if the male fish is small, the dosage of the normal saline can be properly reduced; secondly, injecting 6mg and 6mg of a mixture of oxytocin domperidone and luteinizing hormone releasing hormone analogue (LRH-A) into each kilogram of female fish, and using 2 ml of normal saline and hormone mixed male fish for each female and male fish to reduce the dosage of the normal saline in a proper amount if the individual is small;
8) Effect time: the effect time is 18 hours after one-time injection; two needles are separately injected, the effect time after the first needle injection is 15 hours, and the effect time after the second needle injection is 13 hours;
9) The specific operation method comprises the following steps: catching the parent fish in the parent fish culture pond, and selecting the female fish and the male fish with strong physique and good gonad development by observing and touching the fish body with naked eyes, and putting the female fish and the male fish into a temporary fish tank according to the proportion of 1:3; registering the current time, measuring the current air temperature and water temperature, temporarily taking out the female fish and the male fish from the fish tank, fixing the female fish and the male fish with a device for fixing the parent fish, respectively inserting needles at 45 degrees below pectoral fins of the female fish and the male fish, injecting an oxytocic into the chest cavity, halving the male fish when injecting the oxytocic, controlling the needle insertion depth during injection, leading the needle insertion depth not to work as long as hormone does not work, and leading the needle insertion too deep to prick the heart of the parent fish to cause death of the parent fish; after the injection of the parent fish, putting the parent fish into a spawning pond, covering the spawning pond by using a black net, performing scouring stimulation by using clear micro-flow water, performing artificial spawning induction, and respectively catching the parent fish for artificial fertilization when the effect time is up;
5. artificial fertilization;
1) Artificial insemination apparatus: goose feather, egg-receiving utensil, dry towel, disinfectant (salt, potassium permanganate solution, povidone iodine solution), temporary fish tank, etc.;
2) The operator: more than 3 persons;
3) Room temperature: 25 ℃;
4) Water temperature: 25 ℃;
5) Water quality: close to the national drinking water standard.
6) The specific operation method comprises the following steps: one person controls the parent fish and seals the cloaca of the parent fish by hands at the same time, one person wipes water drops on the abdomen of the parent fish by a dry towel and extrudes eggs and sperms on the abdomen of the parent fish at the same time, the other person uses an egg receiving device to quickly receive the eggs and the sperms, the fertilization of the egg and the sperms can not exceed 1 minute, meanwhile, the eggs and the sperms are fully stirred counterclockwise by goose feathers for 3 minutes to be fully fertilized, then, a proper amount of clear water is added for stirring for 3 minutes again, and the mixture is put into hatching equipment for hatching;
6. artificial incubation;
1) An incubation device: 3 oxygenation pumps of 350W, 50 oxygenation pipes, 50 water pipes, 2 to 4 big barrels of 2 multiplied by 2 meters in diameter, 10 gauze of 40 meshes, 10 vegetable washing baskets of 0.5 multiplied by 1 multiplied by 0.3 meters, one reel of fishing line, 4 bottles of normal saline, a plurality of plastic foams, 2 to 5 thermostats of thermostats, 10 injectors of 20 milliliters and an incubation water tank;
2) The operator: more than 3 people;
3) Room temperature: 25 ℃;
4) Water temperature: 25 ℃;
5) Water quality: close to the national drinking water standard.
6) Oxygen dissolved amount: 6mg/L or more;
7) Demoulding: the demoulding time is 6 days when the water temperature is 22 ℃; the demoulding time is 4 days when the water temperature is 25 ℃; the demoulding time is 3 days when the water temperature is 28 ℃;
8) The specific method of operation: one person puts the self-made hatching floating plate into a hatching water tank, places an oxygen increasing device, one person puts the fertilized eggs into the floating plate to be uniformly distributed, one person uses 3% saline solution to disinfect the water in the hatching tank, goose feathers are used to uniformly stir the fertilized eggs every 3 hours, the water temperature is kept constant, the dissolved oxygen is moderate, the water in the hatching water tank needs to be changed every day, the fertilized eggs which do not develop are selected every day, the fertilized eggs need to develop stably within 48 hours, and after 48 hours, the fish fry is waited to be demoulded;
7. managing in a seedling stage;
1) A management method of demoulding to opening;
controlling the water temperature, keeping the water temperature consistent with the water temperature during incubation, observing whether the oxygen-increasing machine works normally or not, ensuring that the fry is fragile after the fry is demoulded, enabling the fry to move in a mode of moving from the bottom of an incubation net cage to the upper part or the lower part, ensuring that the fry cannot be eaten, only paying attention to constant temperature, increasing oxygen and cleaning the incubation net cage, and starting feeding fry opening materials after the fry is jacked and can move horizontally;
2) A management method of the water bloom seedling stage;
a. controlling the water temperature and observing whether the aerator works normally or not, measuring the water quality every three days, and keeping the water flower seedling culture box clean;
b. feeding: feeding 5 times every day, wherein the bait coefficient is 3 percent, and the purpose of feeding a plurality of meals less is achieved;
c. selecting bait: selecting natural daphnia, soybean milk, salted duck eggs and the like which have protein content of more than 45% and are caught in Tongwei Kouye or a pond;
d. changing water in the seedling pool: once in 5 days, miao Xiangshui is replaced;
e. and (3) disinfection: disinfecting the pond water and the fry once by using a proper amount of potassium permanganate or povidone iodine solution after 5 days;
f. and (3) using a proper amount of the clean fish and insect crystals to fully sprinkle 2000 times of pond water to the seedling pond. 2 times per month, 3-5 days each time, 1-2 days between medication; the florfenicol powder is stirred and fed for 2 times per month, and each time lasts for 3 to 5 days.
g. The feeding method comprises the following steps: follow the four definite principles;
3) Managing method of cun seedling stage;
a. controlling the water temperature, observing whether the aerator works normally or not, measuring the water quality once every 7 days, and keeping the cun-seedling culture pond clean;
b. feeding: feeding for 5 times every day, wherein the bait coefficient is 5 percent, and the purpose of feeding a plurality of meals less is achieved;
a. selecting baits: selecting puffing materials or various vegetable scraps with the protein content of more than 36% and the calibre of Tongwei 1.0 mm;
c. changing water in the seedling pool: replacing the pool water once in 5 days;
d. and (3) disinfection: disinfecting the pond water and the fry once by using a proper amount of potassium permanganate or povidone iodine solution after 5 days;
e. disease control: and (4) using a proper amount of the clean fish and insect crystals to comprehensively sprinkle 2000 times of pond water. 2 times per month, 3-5 days each time, 1-2 days interval; the florfenicol powder is added and fed for 2 times every month for 3 to 5 days.
f. The feeding method comprises the following steps: follow the four rules.
The data of the above examples 1-3 were recorded as follows:
the recorded results show that: the data in the first, second and third examples can successfully catalyze and produce Huang Ke fish eggs, which shows that the method and data provided by the invention have an important effect on the breeding of the yellow-shell fish.
Therefore, the requirements of the yellow-shelled fishes on water quality and temperature are high, yellow-shelled fish eggs cannot be induced according to the dosage required by the conventional fish induced spawning, the survival rate of wild yellow-shelled fishes caught and cultured in the field is low, the wild yellow-shelled fishes are limited in wild quantity and difficult to culture in a large scale, and the economic benefit cannot be improved.
Claims (7)
1. An artificial propagation method for yellow-shelled fishes, which is characterized by comprising the following steps:
1. selecting parent fishes;
selecting 300 female fishes which are artificially domesticated, have strong physique and good development every 2 months, each fish is 2-6 jin, and the age of the fish is more than 4 years old; 900 male fishes, each of which is 0.5 to 2 jin and the age of which is more than 1 year are placed into a parent fish breeding pond for breeding, and the area of the breeding pond is 1 to 3 mu;
2. parent fish feeding;
wheat and barley malt are mainly thrown and fed, various vegetables, fresh fishes and shrimps are fed in a matched mode to increase plant and animal protein, artificial compound feed is not fed, the feeding frequency reaches 3 to 5 times, and the feeding time is as follows: feeding is carried out 7 to 8 am, 11 to 12 am, 15 to 16 pm and 19 to 20 pm, the feeding amount is 8 and full, and the feeding time is one month;
3. establishing an induced spawning and hatching workshop;
1) One workshop with the area of 400 square meters;
2) 1 to 3 per 25 cubic meters of spawning ponds;
3) One 50 cubic meters sedimentation tank;
4) The disinfection tank is 50 cubic meters;
4. artificial hasten parturition;
1) An induced spawning device: a disposable syringe with the volume of 5 ml to 10 ml, normal saline, an egg receiving device, a device for fixing parent fish and a parent fish disinfection pond;
2) An oxytocic drug: chorionic Gonadotropin (HCG), domperidone, and luteinizing hormone-releasing hormone analog (LRH-a);
3) The operator: more than 3 persons;
4) Effect time: the effect time is 16-18 hours after one injection; two needles are separately injected, the effect time after the first needle injection is 14-15 hours, and the effect time after the second needle injection is 12-13 hours;
5) The specific operation method comprises the following steps: catching the parent fish in the parent fish culture pond, and selecting the female fish and the male fish with strong physique and good gonad development by observing and touching the bodies of the parent fish through naked eyes, and putting the female fish and the male fish into the parent fish disinfection pond according to the proportion of 1:3; the current time is registered, the current air temperature and water temperature are measured, female fishes and male fishes are taken out from the parent fish disinfection pool and fixed well by a parent fish fixing device, needles are inserted 45 degrees below pectoral fins of the female fishes and the male fishes respectively, oxytocic is injected into the chest cavity, the needle insertion depth needs to be mastered during injection, hormone does not work enough, and the needles are too deep to prick the heart of the parent fishes, so that the parent fishes die; after the injection of the parent fish, putting the parent fish into a spawning pond, covering the spawning pond by using a black net, performing scouring stimulation by using clear micro-flow water, performing artificial spawning induction, and respectively catching the parent fish for artificial fertilization when the effect time is up;
6) The spawning induction rate: the catalytic yield can reach 90-95% by applying the method;
in the step of artificial oxytocic, the dosage of the oxytocic is injected: injecting one injection, wherein 7 mg and 7 mg of a mixture of oxytocin domperidone and luteinizing hormone releasing hormone analogue (LRH-A) are injected into each kilogram of female fish, each female and male parent fish is mixed with 1-2 ml of normal saline and hormone, and if the male fish is small, the dosage of the normal saline can be reduced properly; two needles are separately injected, the first needle is used for injecting 2 mg and 2 microgram of a mixture of oxytocin domperidone and luteinizing hormone releasing hormone analogue (LRH-A) per kilogram of female fish, each female and male parent fish is mixed with hormone by 1-2 ml of normal saline, and if the male fish is small, the dosage of the normal saline can be properly reduced; secondly, injecting 6mg and 6mg of a mixture of oxytocin domperidone and luteinizing hormone releasing hormone analogue (LRH-A) into each kilogram of female fish, mixing 1-2 ml of normal saline and hormone into each female and male parent fish, and if the male fish is small, reducing the dosage of the normal saline in a proper amount;
5. artificial fertilization;
1) Artificial insemination apparatus: goose feather, egg receiving device, dry towel, salt, potassium permanganate solution, povidone iodine solution and parent fish disinfection pond;
2) The operator: more than 3 persons;
3) The specific operation method comprises the following steps: one person controls the parent fish and seals the cloaca of the parent fish by hands at the same time, one person uses a dry towel to wipe water drops on the abdomen of the parent fish and squeeze eggs and sperms on the abdomen of the parent fish, the other person uses an egg receiving device to quickly receive the eggs and the sperms, the fertilization of the eggs and the sperms cannot exceed 1 minute, meanwhile, goose feather is used for fully stirring the eggs and the sperms anticlockwise for 2-3 minutes to ensure the full fertilization, then a proper amount of clear water is added for stirring for 2-3 minutes, and the mixture is placed into hatching equipment for hatching;
4) Fertilization rate: the fertilization rate can reach 90-95% by applying the method;
6. artificial hatching
1) An incubation device: 2 to 3 oxygenation pumps of 350W, 20 to 50 meters of oxygenation pipes, 20 to 50 meters of water pipes, 2 to 4 big barrels of 2 x 2 meters in diameter, 5 to 10 meters of 40-mesh spun silk, 5 to 10 vegetable washing baskets of 0.5 x 1 meter x 0.3 meter, one roll of fishing line, 4 bottles of physiological saline, a plurality of plastic foams, 2 to 5 thermostats of thermostats, 5 to 10 injectors of 10 to 20 milliliters, and an incubation water tank;
2) The operator: more than 3 persons;
3) Demoulding: the demoulding time is 4 to 6 days when the water temperature is between 20 and 22 ℃; the demoulding time is 3 to 4 days when the water temperature is 24 to 25 ℃; the demoulding time is 3 days when the water temperature is between 26 and 28 ℃;
4) The specific method of operation: one person puts the self-made hatching floating plate into a hatching water tank, an oxygen increasing device is placed, one person puts the fertilized eggs into the floating plate to be uniformly distributed, one person uses 3% saline solution to disinfect the water in the hatching tank, goose down is used for uniformly stirring the fertilized eggs every 2 to 3 hours, the water temperature is kept constant, the dissolved oxygen is moderate, the fertilized eggs need 48 hours to grow stably, and after 48 hours, the fish fry is subjected to demoulding;
5) Hatching rate: by adopting the methods, the hatching rate can reach more than 95 percent;
7. managing in a seedling stage;
1) A management method of demoulding to opening;
controlling the water temperature, keeping the water temperature consistent with the water temperature during incubation, observing whether the oxygen increasing machine works normally, and feeding the opening material of the fry;
2) A management method of the water bloom seedling stage;
a. controlling the water temperature and observing whether the aerator works normally or not, measuring the water quality every three days, and keeping the water flower seedling culture box clean;
b. feeding: feeding for 4 to 5 times every day, wherein the bait coefficient is 2 to 3 percent, and the purpose of feeding a plurality of meals less is achieved;
c. selecting bait: selecting natural daphnia, soybean milk and salted duck eggs with protein content of more than 45% and caught in Tongwei Kouye or a pond;
d. changing water in the seedling pool: once Miao Xiangshui changed for 5 to 7 days;
e. and (3) disinfection: disinfecting the pond water and the fry once by using a proper amount of potassium permanganate or povidone iodine solution in 5 to 7 days;
f. disease control: using a proper amount of the crystal of the ichthyophthirius gifuensis to comprehensively sprinkle 2000 times of pond water to a seedling pond, 2 times a month, 3 to 5 days each time, and 1 to 2 days of medication interval; the florfenicol powder is added and fed for 2 times per month, and each time lasts for 3 to 5 days;
g. the feeding method comprises the following steps: follow the four definite principles;
h. survival rate: the survival rate of the water flower seedlings operated according to the method can reach more than 80 percent;
3) Managing method of cun seedling stage;
a. controlling the water temperature and observing whether the aerator works normally, measuring the water quality once every 7 days, and keeping the cun-seedling culture pond clean;
b. feeding: feeding for 4 to 5 times every day, wherein the bait coefficient is 3 to 5 percent, and the purpose of feeding a plurality of meals less is achieved;
a. selecting baits: selecting puffing materials or various vegetable scraps with the calibers of 1.0 mm of Tongwei and the protein content of which is more than 32-36%;
c. changing water in the seedling pool: replacing the pool water for 5 to 7 days;
d. and (3) disinfection: disinfecting the pond water and the fry once by using a proper amount of potassium permanganate or povidone iodine solution in 5 to 7 days;
e. disease control: using a proper amount of the crystal of the ichthyophthirius gifuensis to comprehensively sprinkle 2000 times of pond water to a seedling pond, 2 times a month, 3 to 5 days each time, and 1 to 2 days of medication interval; the florfenicol powder is added and fed for 2 times per month, and each time lasts for 3 to 5 days;
f. the feeding method comprises the following steps: follow the four definite principles;
g. and (4) survival rate of the effluent from the pool: the survival rate can reach more than 95 percent by the method.
2. An artificial propagation method for yellow-shelled fish according to claim 1, characterized in that: in the artificial spawning induction step, the number of the male fishes is reduced by half when the spawning induction medicine is injected.
3. An artificial propagation method for yellow-shelled fish according to claim 1, characterized in that: in the step of artificial induced spawning, the air temperature is controlled to be 22-32 ℃, and the water temperature is controlled to be 20-28 ℃.
4. An artificial propagation method for yellow-shelled fish according to claim 1, characterized in that: in the artificial fertilization step, the room temperature is controlled to be 22-25 ℃, and the water temperature is controlled to be 24-25 ℃.
5. The artificial propagation method for yellow-shell fish according to claim 1, characterized in that: in the artificial incubation step, the room temperature is controlled to be 22-25 ℃, the water temperature is controlled to be 24-25 ℃, and the oxygen dissolution amount is controlled to be more than 6 mg/L.
6. An artificial propagation method for yellow-shelled fish according to claim 1, characterized in that: in the artificial incubation step, the water in the incubation water vat needs to be changed every day, and fertilized eggs which do not develop are selected every day.
7. An artificial propagation method for yellow-shelled fish according to claim 1, characterized in that: in the management step of the fry period, the fry is fragile after being stripped, the fry can move in a mode of moving from the bottom of the hatching net cage to the upper part or vertically downwards, the fry can not be eaten, the fry can be fed only by paying attention to constant temperature, oxygenation and cleaning of the hatching net cage, and after the fry is jacked to the waist and can move horizontally, the fry opening material is fed.
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009063044A1 (en) * | 2007-11-16 | 2009-05-22 | Pharmaq As | Treatment of parasite diseases using vitamin k3 |
| CN103651220A (en) * | 2013-12-30 | 2014-03-26 | 玉溪市水产工作站（玉溪市水生动物防疫检疫站云南省渔业船舶检验局玉溪检验处） | Barbodes fuxianensis artificial propagation technology |
| CN104221952A (en) * | 2013-12-26 | 2014-12-24 | 江苏省农业科学院宿迁农科所 | Artificial reproduction method of chalcalburnus chalcoides aralensis |
| CN105613382A (en) * | 2016-01-28 | 2016-06-01 | 西南大学 | Complete artificial propagation method of megalobrama pellegrini |
| CN106578087A (en) * | 2016-10-21 | 2017-04-26 | 安徽联喆玉竹有限公司 | Katsumada galangal seed flavored kadsura coccinea milk tea and making method thereof |
| CN111084776A (en) * | 2018-10-24 | 2020-05-01 | 中国科学院昆明动物研究所 | Use of pilenicacid and derivatives thereof for treating and/or preventing neurodegenerative diseases |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103070094B (en) * | 2012-10-16 | 2014-06-25 | 广西壮族自治区海洋研究所 | Artificial breeding method for siganus oramin |
| CN109644902B (en) * | 2018-12-26 | 2021-05-07 | 中国水产科学研究院长江水产研究所 | Artificial breeding method for schizothorax lasanensis |
-
2021
- 2021-07-05 CN CN202110757484.4A patent/CN113287548B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009063044A1 (en) * | 2007-11-16 | 2009-05-22 | Pharmaq As | Treatment of parasite diseases using vitamin k3 |
| CN104221952A (en) * | 2013-12-26 | 2014-12-24 | 江苏省农业科学院宿迁农科所 | Artificial reproduction method of chalcalburnus chalcoides aralensis |
| CN103651220A (en) * | 2013-12-30 | 2014-03-26 | 玉溪市水产工作站（玉溪市水生动物防疫检疫站云南省渔业船舶检验局玉溪检验处） | Barbodes fuxianensis artificial propagation technology |
| CN105613382A (en) * | 2016-01-28 | 2016-06-01 | 西南大学 | Complete artificial propagation method of megalobrama pellegrini |
| CN106578087A (en) * | 2016-10-21 | 2017-04-26 | 安徽联喆玉竹有限公司 | Katsumada galangal seed flavored kadsura coccinea milk tea and making method thereof |
| CN111084776A (en) * | 2018-10-24 | 2020-05-01 | 中国科学院昆明动物研究所 | Use of pilenicacid and derivatives thereof for treating and/or preventing neurodegenerative diseases |
Non-Patent Citations (2)
| Title |
|---|
| 保山四须的生物学特性研究;张建斌等;《淡水渔业》;20030315(第02期);第52-53页 * |
| 孔令富等.保山四须鲃人工繁殖技术研究.《水产养殖》.2020,(第02期), * |
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