CN101743924B - In-vitro hatching method for freshwater lobster zygote - Google Patents
In-vitro hatching method for freshwater lobster zygote Download PDFInfo
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- CN101743924B CN101743924B CN2009102648889A CN200910264888A CN101743924B CN 101743924 B CN101743924 B CN 101743924B CN 2009102648889 A CN2009102648889 A CN 2009102648889A CN 200910264888 A CN200910264888 A CN 200910264888A CN 101743924 B CN101743924 B CN 101743924B
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- zygote
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention discloses an in-vitro hatching method for freshwater lobster zygote. The technical scheme comprises the steps of: putting oogenesis parent lobsters fished in batch into a methylene blue solution to be soaked for 5-15 minutes; lightly stripping zygotes from parent lobster appendages by using sterile tweezers, and putting into a zygote hatcher; and hatching into post larvae after 16-26 days, wherein the hatching rate of the zygote can achieve more than 95%. The method has the advantages of large zygote amount, little occupation land for zygote in-vitro hatching, little water using amount, labors and materials saving, no need of continuous breeding of the oogenesis lobster, and reduction of feeding management cost of the oogenesis lobster. The embryo can survive, and quickly hatched into larvae by adopting the in-vitro hatching method; the size of the larvae is uniform, and the commercial value is high; the mutual killing phenomenon among the larvae can be effectively prevented, and the survival rate of larvae is enhanced; and the in-vitro hatching method is suitable for the in-vitro hatching of scaled freshwater lobster zygotes.
Description
Technical field
The present invention relates to aquaculture technology, be specifically related to a kind of in-vitro hatching method for freshwater lobster zygote.
Background technology
Freshwater lobster formal name used at school Procambius clarkii, because its wide adaptability, fertility is strong, and no matter all can live in rivers, lake, pond and paddy field, and its vitality is extremely strong, and its survival and development all suits in the most of places of China.
Because the apparent consumption of freshwater lobster and the quick growth of export processing quantity; Its wild resource quantity sharply reduces, and the output that therefore increases freshwater lobster through the method for propagating artificially has become one of important channel of satisfying export processing and consumption market.In recent years; The sharp increase of As market amount, the market price rises year by year, has excited the masses to culture the enthusiasm of Procambius clarkii; Most of raiser does not understand the Procambius clarkii cultural technique very much; Cultured output and benefit are generally not high and extremely unstable, especially culture the seed problem, have restricted the development of Procambius clarkii breeding production.
Existing technology is gathered wild shrimp with ovums, is invested in the simulation wild environment, treat the hatching of shrimp ovum after, closeer shrimp and young shrimp are separated, separately young shrimp is cultivated then.This cultivation technical modelling wild environment is difficult to the formation scale, and young shrimp grows asynchronous, and shrimp seedling specification is irregular, causes the shrimp seedling probability that cuts one another's throat sharply to raise, and has had a strong impact on the survival rate of young shrimp.
Summary of the invention
The objective of the invention is to overcome weak point in the prior art, a kind of in-vitro hatching method for freshwater lobster zygote is provided.
In-vitro hatching method for freshwater lobster zygote: the oogenesis parent shrimp after fishing for is in batches put into methylene blue solution soaked for 5～15 seconds; Separate fertilized egg from close shrimp appendage lightly with aseptic nipper and put into oosperm hatcher, through hatching into young shrimp in 16～26 days.
The present invention is achieved through following technical proposals:
In-vitro hatching method for freshwater lobster zygote: water filling is carried out to the pond in (1) in the pond of large-scale cultivation, and pond waters is flowed, and the instruments of fishing for such as land used cage carry out batch and fish for oogenesis parent shrimp; (2) oogenesis parent shrimp is put into methylene blue solution and soaked for 5～15 seconds, the concentration of methylene blue solution is controlled at 0.5～5mg/L; (3) separate fertilized egg from close shrimp appendage lightly with aseptic nipper and put into oosperm hatcher; (4) with fresh water as the oosperm hatcher source of recycled water, the depth of water in the oosperm hatcher is 10～100cm, the pH value is controlled at 6.5～8.5, water temperature is controlled at 15～25 ℃, circulating water flow is 1～10m
3/ h puts into the penicillin for animals of 20～3,200,000 units in cycle water, intensity of illumination is 500Lux～5000Lux, and light application time is 6～12 hours, hatches into young shrimp through 16～26 days, and the fertilized egg hatching rate can reach more than 95%.
Said oosperm hatcher is to adopt the general oosperm hatcher of a kind of fish.
In-vitro hatching method for freshwater lobster zygote has that the fertilized egg amount that can hatch is big, the fertilized egg in-vitro hatching takes up an area of less, water consumption is few, use manpower and material resources sparingly, need not to continue the characteristics of the feeding and management cost cultivating shrimp with ovums and can reduce shrimp with ovums.
In-vitro hatching method for freshwater lobster zygote not only can make the embryo become to live, and hatching is the young rapidly, but also can avoid causing embryonic death owing to close shrimp is unhealthy, makes hatchability of fertile eggs be higher than prior art;
The young shrimp neat specification that adopts in-vitro hatching method for freshwater lobster zygote to hatch, commodity value is high; And can effectively prevent the phenomenon that cuts one another's throat between young shrimp, improved the survival rate of young shrimp;
In-vitro hatching method for freshwater lobster zygote is applicable to the in-vitro hatching of scale freshwater lobster zygote.
Embodiment
Below in conjunction with embodiment the present invention is done further description:
In-vitro hatching method for freshwater lobster zygote: water filling is carried out to the pond in (1) in the pond of large-scale cultivation, and pond waters is flowed, and the instruments of fishing for such as land used cage carry out batch and fish for oogenesis parent shrimp; (2) oogenesis parent shrimp is put into methylene blue solution and soaked for 10 seconds, the concentration of methylene blue solution is controlled at 1～2mg/L; (3) separate fertilized egg from close shrimp appendage lightly with aseptic nipper and put into oosperm hatcher; (4) with fresh water as the oosperm hatcher source of recycled water, the depth of water in the oosperm hatcher is 60cm, the pH value is controlled at 6.5～7.5, water temperature is controlled at 20～22 ℃, circulating water flow is 3m
3/ h puts into the penicillin for animals of 1,600,000 units in cycle water, intensity of illumination is 2000Lux, and light application time is 8 hours, through hatching into young shrimp in 20～24 days.
Claims (1)
1. an in-vitro hatching method for freshwater lobster zygote is characterized in that, water filling is carried out to the pond in (1) in the pond of large-scale cultivation, and pond waters is flowed, and the land used cage instrument of fishing for carries out batch and fishes for oogenesis parent shrimp; (2) oogenesis parent shrimp is put into methylene blue solution and soaked for 5～15 seconds, the concentration of methylene blue solution is controlled at 0.5～5mg/L; (3) separate fertilized egg from close shrimp appendage lightly with aseptic nipper and put into oosperm hatcher; (4) with fresh water as the oosperm hatcher source of recycled water, the depth of water in the oosperm hatcher is 10～100cm, the pH value is controlled at 6.5～8.5, water temperature is controlled at 15～25 ℃, circulating water flow is 1～10m
3/ h puts into the penicillin for animals of 20～3,200,000 units in cycle water, intensity of illumination is 500Lux～5000Lux, and light application time is 6～12 hours, hatches into young shrimp through 16～26 days, and the fertilized egg hatching rate can reach more than 95%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009102648889A CN101743924B (en) | 2009-12-25 | 2009-12-25 | In-vitro hatching method for freshwater lobster zygote |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009102648889A CN101743924B (en) | 2009-12-25 | 2009-12-25 | In-vitro hatching method for freshwater lobster zygote |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101743924A CN101743924A (en) | 2010-06-23 |
| CN101743924B true CN101743924B (en) | 2012-02-22 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2009102648889A Expired - Fee Related CN101743924B (en) | 2009-12-25 | 2009-12-25 | In-vitro hatching method for freshwater lobster zygote |
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Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103004656A (en) * | 2012-12-17 | 2013-04-03 | 南充市营渔水产科技有限公司 | Intensified artificial breeding method for Australian crayfishes |
| CN103314908A (en) * | 2013-06-21 | 2013-09-25 | 太仓年年有渔农业科技有限公司 | Isolated breeding technique for cherax quadricarinatus eggs |
| CN103636541A (en) * | 2013-12-18 | 2014-03-19 | 上海市水产研究所 | In-vitro incubation method for fertilized eggs of macrobrachium rosenbergii |
| CN107568122A (en) * | 2017-09-21 | 2018-01-12 | 江苏盱眙满江红龙虾产业园有限公司 | A kind of Procambius clarkii embryonated egg in-vitro hatching method |
| CN107361002A (en) * | 2017-09-21 | 2017-11-21 | 江苏盱眙满江红龙虾产业园有限公司 | A kind of Procambrus clarkii ovum in-vitro culture method |
| CN110326568A (en) * | 2019-08-15 | 2019-10-15 | 中国水产科学研究院淡水渔业研究中心 | A kind of Procambius clarkii early stage shrimp ovum in-vitro breeding method |
| CN111328742B (en) * | 2020-03-03 | 2021-12-14 | 天津农学院 | Method for improving hatching rate of in-vitro culture of embryos of young shrimps with saw teeth |
| CN113557992A (en) * | 2020-06-02 | 2021-10-29 | 海南海壹水产种苗有限公司 | Device and method for hatching fertilized eggs of shrimps in running water |
| CN112544511B (en) * | 2020-12-09 | 2024-06-25 | 中国科学院水生生物研究所 | Device and method for in-vitro hatching of fertilized eggs of crayfish |
| CN115005134B (en) * | 2022-06-14 | 2023-07-28 | 海南省菜篮农业与渔业发展有限公司 | In-vitro hatching method for freshwater shrimps |
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2009
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| CN101743924A (en) | 2010-06-23 |
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Granted publication date: 20120222 Termination date: 20181225 |