CN103636541A - In-vitro incubation method for fertilized eggs of macrobrachium rosenbergii - Google Patents

In-vitro incubation method for fertilized eggs of macrobrachium rosenbergii Download PDF

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Publication number
CN103636541A
CN103636541A CN201310696505.1A CN201310696505A CN103636541A CN 103636541 A CN103636541 A CN 103636541A CN 201310696505 A CN201310696505 A CN 201310696505A CN 103636541 A CN103636541 A CN 103636541A
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China
Prior art keywords
water
aquarium
hatching
incubation
fertilized eggs
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CN201310696505.1A
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Chinese (zh)
Inventor
陆根海
刘建忠
施永海
张海明
邓平平
谢永德
徐嘉波
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SHANGHAI AQUATIC PRODUCT TECHNOLOGY PROMOTION STATION
Shanghai Fisheries Research Institute
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SHANGHAI AQUATIC PRODUCT TECHNOLOGY PROMOTION STATION
Shanghai Fisheries Research Institute
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Priority to CN201310696505.1A priority Critical patent/CN103636541A/en
Publication of CN103636541A publication Critical patent/CN103636541A/en
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish

Abstract

An in-vitro incubation method for fertilized eggs of macrobrachium rosenbergii adopts fertilized eggs which are stripped out of giant freshwater prawn parent bodies, and is characterized in that fertilized eggs stripped out of the parent bodies are subjected to incubation at the constant temperature, the releasing density of the fertilized eggs is 100-1,000 grains/L, water for incubation adopts natural seawater, salinity of the natural seawater is 10 permillage to 12 permillage, the water temperature ranges from 27 DEG C to 29 DEG C, and the density of air inflation heads placed in an aquarium or an incubation cylinder is 3-4 / m <2>; air inflation is kept in boiling state, more than two thirds of water is changed every day, and the water changing temperature difference does not exceed 0.5 DEG C; development conditions of the fertilized eggs are checked at any time, bad eggs, mildewed eggs and sewage in a water body are rejected in time until zoaea is developed, and the zoaea is separated in time and counted; the air inflation heads are taken out, and after the water body is rotated and subjected to stand still for minutes, egg grains and egg mass sunk to the bottom are sucked by a siphon; the water body and larvae in the aquarium or the incubation cylinder are siphoned into a 80-mesh net pocket for counting; and finally, the collected egg grains and egg mass are put into the aquarium or the incubation cylinder for continuous incubation by supplementing water with the same temperature and the same salinity.

Description

The in-vitro hatching method of Macrobrachium rosenbergii fertilized egg
Technical field
The present invention relates to the hatching m of Macrobrachium rosenbergii fertilized egg, especially depart from the hatching m of the Macrobrachium rosenbergii fertilized egg of parent.
Background technology
Macrobrachium rosenbergii ( macrobrachium rosenbergii) from the 1980s, introduce domestic, as a kind of large-scale economic freshwater shrimps, because it has that growth is fast, wide adaptability, premunition be strong, to bait requirement is low and nutritious and palatability is good, thereby welcome by citizen.At present, from artificial breeding, still cultivate and all obtained huge success, and formed perfect industrial system.But, through the development of nearly 30 years, during run into again the problem as other breed variety: poor growth, a series of deterioration of variety signs such as individual difference is serious, disease increases.In recent years, people by distant hybridization, the method such as introduce a fine variety is improved germplasm again, has obtained obvious effect, fact proved, this method is feasible, is also effective.Yet, implement this scheme of improving germplasm, certainly will will pass through parent's long-distance live transport, and this work is by a large amount of human and material resources and the financial resources of cost.
Summary of the invention
The in-vitro hatching method that the object of this invention is to provide a kind of Macrobrachium rosenbergii fertilized egg.
Technical scheme of the present invention is to adopt the fertilized egg of peeling off Macrobrachium rosenbergii parent, it is characterized in that the fertilized egg of peeling off Macrobrachium rosenbergii parent to put into aquarium or hatch cylinder to carry out Constant temperature hatch, it is 100-1000 grain/L that fertilized egg is thrown in density, Spawning water is for the Low-salinity natural sea-water through one-level precipitation is through 130 eye mesh screen bag filtrations, salinity is 10-12 ‰, water temperature is controlled at 27-29 ℃, in aquarium or hatching cylinder, place 3-4/㎡ of pneumatic head, keep continuous charge and guarantee that aeration quantity reaches boiling shape, to avoid ovum grain to sink to the bottom, change water every day more than 2/3, change water temperature difference and be no more than 0.5 ℃, check at any time development of fertilized ova situation and reject in time bad ovum, dirt in mould ovum and water body, until find that there is, zoea is separated in time also to be counted, because the young is many, at night, hatch, therefore, the separation of the young and counting can combine and carry out with the water that changes of every morning, concrete grammar is: take out pneumatic head, rotation water body is also after static several minutes, and ovum grain, the pieces of an egg that sink to the bottom with siphon pipe sucking-off also collect, then water body in aquarium or hatching cylinder is taken in to 80 order mesh bags together with young siphon and count, finally ovum grain, the pieces of an egg originally collected are dropped in aquarium or hatching cylinder again, fill into equality of temperature, with the water continuation of salt, hatch.
The present invention is to have created to utilize from oogenesis Macrobrachium rosenbergii parent, to separate fertilized egg with it and carry out cultured in vitro, hatching with respect to the outstanding feature of prior art, be different from the live body hatching that existing Macrobrachium rosenbergii artificial breeding pattern is shrimp with ovums, while especially avoiding introducing a fine variety, need a large amount of human and material resources, financial resources to go to carry out parent's live transport.And experimental result of the present invention shows, particularly scientific research is with introducing a fine variety on a small scale, only need select suitable shrimp with ovums introducing a fine variety, and separates on the spot oosperm block and puts into oxygen bag, and handbag is taken away, and a few hours arrival can be put into ready water body and be carried out in-vitro hatching.In-vitro hatching rate can reach more than 30%.Both saved a large amount of human and material resources, simple again.
Embodiment
Embodiment mono-, concrete implementation step is carried out in the following manner: the shrimp with ovums from the shrimp with ovums of living or firm dead 3-5 hour and in the sufficient water body of dissolved oxygen separates oosperm block, weigh and fully shred with scissors, make ovum grain be difficult for sinking to the bottom, then by the density of 100-1000 grain/L, throw in the aquarium of 100L water body or hatch in cylinder and hatch, aquarium or the hatching cylinder top head black film that shelters from heat or light, Spawning water is to be the Low-salinity seawater of 10-12 ‰ through one-level precipitation the salinity of filtering through 130 eye mesh screens, in aquarium or hatching cylinder, press 3-4/㎡ density and place gas head, continuous charge also makes inflation reach boiling shape, to avoid ovum grain to sink to the bottom, progressively heat and temperature control to 27-29 ℃. check development of fertilized ova situation every day and change water more than 2/3, reject in time dead ovum, other dirt in bad ovum and water body, change water temperature difference and be no more than 0.5 ℃, when inspection finds that there is zoea, in time the young is separated and counted, separating method is: take out pneumatic head, rotation water body, after 3-5 minute, with siphonage, will sink to the bottom ovum grain, pieces of an egg suck in 80 order soil pick-up frames, soil pick-up frame is placed in basin to avoid dehydration, again zoea band water is sucked together in 80 order mesh bags and counted, and being returned in aquarium or hatching cylinder, the not hatching fertilized egg of sucking-off before fills into equality of temperature, water with salt continues hatching.Said method is in seed base, Nikkei Shanghai Aquatic Product Institute Fengxian experiment March 26 to April 1 in 2012, the pieces of an egg of ten number tail shrimp with ovums are peeled off out, carry out in-vitro hatching and cultivation, hatch altogether and isolate zoea more than 10000 tails, to April 15, began to see that young shrimp, April 17 rose to April 22, isolated altogether young shrimp more than 4600 tails and move into great Chi and raise separately.To May 17, again went out pond and put in a suitable place to breed and be counted as 4000 tails, November 20 catching amount 3100 tails, maximum individuality reaches 88g, overall culture efficiency is obvious.
Embodiment bis-, according to the method described in embodiment mono-, carry out, the in vitro timeliness experimental data of fertilized egg is as follows: 2013 annual interests are with forming the also Macrobrachium rosenbergii original seed first filial generation of overwintering culture last year, April 17, having selected 6 tail oogenesis times is the shrimp with ovums of 1-7 days, the heavy 34.5g of average shrimp, the heavy 4g of ovum, ovum number is 8930/g, after fully stirring evenly, be divided into two, half is that 10.7 ten thousand of 12g meters directly carry out in-vitro hatching in input hatching cylinder; Second half compares, and puts into oxygen bag insert and drop into hatching cylinder after the low temperature water-bath 8h of 15 ℃ again and carry out in-vitro hatching with water.Hatching cylinder volume is 100L, and it is 1070/L that fertilized egg is thrown in density, the natural brackish water that salinity is 12 ‰, and temperature control 27-29 ℃ between the incubation period, changes water every day more than 2/3.Collect respectively the young number April 29 to May 5 is 3.5 ten thousand tails and 0.7 ten thousand tails, and cultured in vitro incubation rate is respectively 32.7 ﹪ and 6.5 ﹪.

Claims (1)

1. the in-vitro hatching method of Macrobrachium rosenbergii fertilized egg, the shrimp with ovums of employing from the shrimp with ovums of living or firm dead 3-5 hour and in the sufficient water body of dissolved oxygen separates oosperm block, it is characterized in that the oosperm block of peeling off Macrobrachium rosenbergii parent to shred, make ovum grain be difficult for sinking to the bottom, then by the density of 100-1000 grain/L, throw in aquarium or hatching cylinder and hatch, aquarium or the hatching cylinder top head black film that shelters from heat or light, Spawning water is to be the Low-salinity seawater of 10-12 ‰ through one-level precipitation the salinity of filtering through 130 eye mesh screens, in aquarium or hatching cylinder, press 3-4/㎡ density and place gas head, continuous charge also makes inflation reach boiling shape, to avoid ovum grain to sink to the bottom, progressively heat and temperature control to 27-29 ℃. check development of fertilized ova situation every day and change water more than 2/3, reject in time dead ovum, dirt in bad ovum and water body, change water temperature difference and be no more than 0.5 ℃, when inspection finds that there is zoea, in time the young is separated and counted, separating method is: take out pneumatic head, rotation water body, after 3-5 minute, with siphonage, will sink to the bottom ovum grain, pieces of an egg suck in 80 order soil pick-up frames, soil pick-up frame is placed in basin to avoid dehydration, again zoea band water is sucked together in 80 order mesh bags and counted, and being returned in aquarium or hatching cylinder, the not hatching fertilized egg of sucking-off before fills into equality of temperature, water with salt continues hatching.
CN201310696505.1A 2013-12-18 2013-12-18 In-vitro incubation method for fertilized eggs of macrobrachium rosenbergii Pending CN103636541A (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107568122A (en) * 2017-09-21 2018-01-12 江苏盱眙满江红龙虾产业园有限公司 A kind of Procambius clarkii embryonated egg in-vitro hatching method
CN110250056A (en) * 2019-07-10 2019-09-20 淮阴师范学院 A method of improving Macrobrachium nipponensis fertilized eggs in-vitro hatching rate
CN111304145A (en) * 2020-03-03 2020-06-19 天津农学院 Method for improving in vitro culture hatchability of macrobrachium rosenbergii embryos
CN113016680A (en) * 2021-03-29 2021-06-25 广西壮族自治区水产科学研究院 Method for high-health reproduction and larva hatching of prawns

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CN103960182A (en) * 2014-04-28 2014-08-06 江苏省海洋水产研究所 Method for establishing exopalaemon carinicauda family by applying in-vitro egg culture

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Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107568122A (en) * 2017-09-21 2018-01-12 江苏盱眙满江红龙虾产业园有限公司 A kind of Procambius clarkii embryonated egg in-vitro hatching method
CN110250056A (en) * 2019-07-10 2019-09-20 淮阴师范学院 A method of improving Macrobrachium nipponensis fertilized eggs in-vitro hatching rate
CN110250056B (en) * 2019-07-10 2021-09-24 淮阴师范学院 Method for improving in vitro hatchability of fertilized eggs of macrobrachium nipponense
CN111304145A (en) * 2020-03-03 2020-06-19 天津农学院 Method for improving in vitro culture hatchability of macrobrachium rosenbergii embryos
CN111304145B (en) * 2020-03-03 2022-06-10 天津农学院 Method for improving in vitro culture hatchability of macrobrachium rosenbergii embryos
CN113016680A (en) * 2021-03-29 2021-06-25 广西壮族自治区水产科学研究院 Method for high-health reproduction and larva hatching of prawns

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Application publication date: 20140319