CN102599095B - Estrus and spawning induction method for increasing rate of artificial insemination of Schizothorax davidi (Sauvage) - Google Patents
Estrus and spawning induction method for increasing rate of artificial insemination of Schizothorax davidi (Sauvage) Download PDFInfo
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- CN102599095B CN102599095B CN201210105693.1A CN201210105693A CN102599095B CN 102599095 B CN102599095 B CN 102599095B CN 201210105693 A CN201210105693 A CN 201210105693A CN 102599095 B CN102599095 B CN 102599095B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention provides an estrus and spawning induction method for increasing the rate of artificial insemination of Schizothorax davidi (Sauvage), which includes estrus induction of parent fishes, spawning induction and insemination. The method is characterized by including: firstly, well mixing pituitary excretion of mature fishes of Schizothorax davidi (Sauvage) and distilled water according to a mass ratio of 1:50-70 before parent fishes of Schizothorax davidi (Sauvage) lay eggs, and injecting the male and female parent fishes with the mixture to induce estrus; secondly, injecting spawning liquid to the root of a pectoral fin of each parent fish to induce spawning when genital orifices of the female parent fishes flush slightly or tail fins of the female parent fishes flush under observation; and thirdly, collecting sperms and the eggs for insemination, mixing the sperms and the eggs according to a ratio of 1.5-2:1, and placing the mixture into fresh water for cleaning.
Description
Technical field
The invention belongs to a kind of artificial incubation method of heavy mouth schizothoracin, be specifically related to improve the induced spawning method of promoting the sexual maturity of heavy mouth schizothoracin artificial breed rate.
Background technology
Heavy mouth schizothoracin (Schizothorax (Racoma) davidi) belongs to Cypriniformes, Cyprinidae, Schizothoracinae, Schizothorax, splits buttocks fish subgenus, popular name: Chong Kou, heavy lip schizothoracin, it is the rare indigenous cold water fishes of China, because of its fine and tender taste, meat flavour deliciousness, is favored by consumers in general deeply.Sexual maturity when three age of wild heavy mouth schizothoracin milter, sexual maturity when four age of raun, when adverse current is to distributary from each great river in August, lay eggs, stimulate and lay eggs by the extruding of water temperature and milter, so egg-laying time is not concentrated, particularly be subject to the impact of surrounding environment, seed distribution randomness is strong, and the fertilization rate of ovum is not high, the impact of the factor such as be in addition subject to that Environmental variations, mankind's transition are fished for, heavy mouth schizothoracin Natural Population reduces in a large number, endangered.。
People start the artificial incubation method of propagating heavy mouth schizothoracin artificially and beginning one's study heavy rictus abdomen fish parent fish in recent years, the reproduction rate of heavy rictus abdomen fish parent fish improves, but adopt under spontaneous estrus situation, artificial exerting pressure gather fish-egg and milt submethod, cause like this seminal fluid and maturation of ovum rate not high, thereby make the insemination rate of fish-egg not high.
Summary of the invention
The object of the invention is for the not high problem of current heavy mouth schizothoracin fertilization rate, a kind of artificial incubation of the heavy mouth schizothoracin that the improves insemination rate induced spawning method of promoting the sexual maturity is provided.
The present invention is achieved in that and comprises and promote the sexual maturity, hasten parturition and inseminate, it is characterized in that:
1, promote the sexual maturity: in 7～August, the sexually matured heavy mouth schizothoracin choosing is put into parent fish pond flowing water and put in a suitable place to breed, high 180～200 ㎝ in parent fish pond pond, the depth of water 60～80 ㎝, the water temperature of flowing water is that 9～15 ℃, dissolved oxygen amount are that 6～10 ㎎/L, pH value are 6.5～7.5; Before laying eggs in 40～50 hours, ripe carp brain hypophysis is after 1:50～70 mix in mass ratio with distilled water, be expelled to the pectoral fin root of male and female parent population, carp brain hypophysis and distilled water mixed liquor injection volume are that female parent population is by 2～3% of body weight, male parent population is by body weight 0.8～1.2%, and after injection, male and female parent population is separately put in a suitable place to breed.
2, hasten parturition: observe the gonopore of the female parent population of heavy rictus abdomen slightly rubescent or when male parent population mouth has corn to occur and tail fin reddens, at the parent population pectoral fin root place injection liquid of hastening parturition, male parent population injection 1 time, female parent population injection twice, the time interval is 10～16 hours; The liquid of hastening parturition be human chorionic gonadtropin (HCG) and maleic acid DOM (DOM) according to the preparation of the ratio of volume ratio 1:0.8～1.2, the injection volume of the liquid of hastening parturition be male parent population by body weight 0.8～1.2%, female parent population is by body weight 1.8～2.4%; After injection, male and female parent population is separately put in a suitable place to breed.
3, insemination: when parent population starts to lay eggs, gather immediately fish-egg and milt, after milt and fish-egg mix by the ratio of 1.5～2:1 in basin with feather stirring, after half a minute, put into clear water rinsing, until fish-egg each and every one absorbs water full and epidermis seminal fluid mucus completely without.
Use heavy mouth schizothoracin of the present invention to promote the sexual maturity and induced spawning method, promoted the gonad development of parent population, improved the maturing rate of the interior ovum of parent population body or sperm, the spawning rate of milter reaches 92%, the spawning rate of raun reaches 60%, has improved the synchronism of male parent population ejaculation and female parent population ovulation period simultaneously.Use the present invention, effectively improved the quality and quantity of the interior sperm of parent population body and ovum, promoted the raising of insemination rate, the unit cost of production effectively reducing.More concentrated because of egg-laying time, can realize the requirement of heavy mouth schizothoracin artificial incubation suitability for industrialized production.
embodiment:
1, promote the sexual maturity: put in a suitable place to breed selecting individual sexually matured heavy mouth schizothoracin even, that build is graceful, vigor is stronger to put into parent fish pond flowing water in 7～August, high 180～200 ㎝ in parent fish pond pond, the depth of water 60～80 ㎝, the water temperature of flowing water is that 9～15 ℃, dissolved oxygen amount are that 6～10 ㎎/L, pH value are 6.5～7.5; Before laying eggs in 40～50 hours, ripe carp brain hypophysis is after 1:50～70 mix in mass ratio with distilled water, be expelled to the pectoral fin root of male and female parent population, carp brain hypophysis and distilled water mixed liquor injection volume are that female parent population is by 2～4% of body weight, male parent population is by body weight 1～2%, and after injection, male and female parent population is separately put in a suitable place to breed.
2, hasten parturition: slight when rubescent at the gonopore of observing raun, injection ocyodinic, guarantees that gather the unified time of fish-egg, when milter tail fin reddens, also injects ocyodinic, promotes the generation of seminal fluid; Inject the liquid of hastening parturition at parent population pectoral fin root place, male parent population injection 1 time, female parent population injection twice, the time interval is 10～16 hours; The liquid of hastening parturition be human chorionic gonadtropin (HCG) and maleic acid DOM (DOM) according to the preparation of the ratio of volume ratio 1:0.8～1.2, the injection volume of the liquid of hastening parturition be male parent population by body weight 0.8～1.2%, female parent population is by body weight 1.8～2.4%; After injection, male and female parent population is separately put in a suitable place to breed.
3, insemination: the parent population of injecting ocyodinic generally starts to lay eggs about 24 hours, the apparatus such as the feather that is ready to sterilize, half dry towel, basin, find that parent population has that the unusual and tail fin of a some mood is complete can adopt ovum semen collection when rubescent, use half dry towel under tail on female milter head is snarled and mentioned, and dab the belly of fish along fish body with hand, gather fish-egg and milt; After milt and fish-egg mix by the ratio of 1.5～2:1 in basin with feather stirring, after half a minute, put into clear water rinsing, until fish-egg each and every one absorbs water full and epidermis seminal fluid mucus completely without.
Claims (1)
1. a method that improves heavy mouth schizothoracin artificial breed rate, is characterized in that step is as follows:
(1) promote the sexual maturity: in 7～August, the sexually matured heavy mouth schizothoracin choosing is put into parent fish pond flowing water and put in a suitable place to breed, high 180～200 ㎝ in parent fish pond pond, the depth of water 60～80 ㎝, the water temperature of flowing water is that 9～15 ℃, dissolved oxygen amount are that 6～10 ㎎/L, pH value are 6.5～7.5; Before laying eggs in 40～50 hours, ripe carp brain hypophysis is after 1:50～70 mix in mass ratio with distilled water, be expelled to the pectoral fin root of male and female parent population, carp brain hypophysis and distilled water mixed liquor injection volume are that female parent population is by 2～3% of body weight, male parent population is by body weight 0.8～1.2%, and after injection, male and female parent population is separately put in a suitable place to breed;
(2) hasten parturition: observe the gonopore of the female parent population of heavy rictus abdomen slightly rubescent or when male parent population mouth has corn to occur and tail fin reddens, inject the liquid of hastening parturition at parent population pectoral fin root place, male parent population injection 1 time, female parent population injection twice, the time interval is 10～16 hours; The liquid of hastening parturition be human chorionic gonadtropin (HCG) and maleic acid DOM (DOM) according to the ratio preparation of volume ratio 1:0.8～1.2, the injection volume of the liquid of hastening parturition be male parent population by body weight 0.8～1.2%, female parent population is by body weight 1.8～2.4%; After injection, male and female parent population is separately put in a suitable place to breed;
(3) insemination: gather immediately fish-egg and milt when parent population starts to lay eggs, milt and fish-egg stir with feather after mixing in the ratio of 1.5～2:1 in basin, after half a minute, put into clear water rinsing, until fish-egg each and every one absorbs water full and epidermis seminal fluid mucus completely without.
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| CN201210105693.1A CN102599095B (en) | 2012-04-12 | 2012-04-12 | Estrus and spawning induction method for increasing rate of artificial insemination of Schizothorax davidi (Sauvage) |
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| CN201210105693.1A CN102599095B (en) | 2012-04-12 | 2012-04-12 | Estrus and spawning induction method for increasing rate of artificial insemination of Schizothorax davidi (Sauvage) |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103651193A (en) * | 2013-11-11 | 2014-03-26 | 青岛佰众化工技术有限公司 | Artificial induced spawning fertilization method of leiocassis longirostris |
| CN103734044A (en) * | 2013-12-18 | 2014-04-23 | 华中农业大学 | Parturition hastening method for plateau-fish schizothorax oconnori |
| CN104082206A (en) * | 2014-06-23 | 2014-10-08 | 华中农业大学 | Spawning induction method of highland oxygymnocypris stewartii |
| CN105941220A (en) * | 2016-05-16 | 2016-09-21 | 塔里木大学 | Method for increasing survival rate of wild schizothorax biddulphi in the manner of pond culture |
| CN109644902B (en) * | 2018-12-26 | 2021-05-07 | 中国水产科学研究院长江水产研究所 | Artificial breeding method for schizothorax lasanensis |
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| JP2011067187A (en) * | 2009-09-25 | 2011-04-07 | Kagawa Prefecture | Artificial insemination method of internal fertilization fish of marine fish |
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