CN103918602A - Efficient artificial breeding technology for giant salamanders - Google Patents
Efficient artificial breeding technology for giant salamanders Download PDFInfo
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- CN103918602A CN103918602A CN201410155454.6A CN201410155454A CN103918602A CN 103918602 A CN103918602 A CN 103918602A CN 201410155454 A CN201410155454 A CN 201410155454A CN 103918602 A CN103918602 A CN 103918602A
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- giant salamander
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Abstract
The invention discloses an efficient artificial breeding technology for giant salamanders and relates to the technical field of the giant salamander breeding. The efficient artificial breeding technology comprises the following steps of mixing aphrodisiac injection, conducting classified injection according to sexual maturity, manually combining fertilized eggs, cleaning the fertilized eggs and conducting incubation in an incubating pool. According to the efficient artificial breeding technology for the giant salamanders, pure artificial breeding and pure wild imitation breeding are combined, the problems that sexual development is poor when only the pure artificial breeding is adopted, and male and female giant salamanders are non-synchronous when only the pure wild imitation breeding is adopted are solved, and breeding efficiency and quality of the giant salamanders are improved by the combination of the pure artificial breeding and the pure wild imitation breeding.
Description
Technical field:
The present invention relates to giant salamander cultural technique field, be specifically related to the high-efficiency artificial raising technology of a kind of giant salamander.
Background technology:
The giant salamander is commonly called as giant salamander, be in the world existing maximum be also most precious amphibian, its cry is the spitting image of child's sob, therefore people cry again it " giant salamander ", national two class protection aqua-marine lifes, agriculture industrialization and featured agriculture focus development kind, it is wildlife gene protection kind, Chinese giant salamander, Andrias davidianus is except Xinjiang, Tibet, Inner Mongol, Taiwan has no outside report, there is distribution all the other provinces and regions, mainly originate in the Changjiang river, in the mountain stream streams in the Yellow River and Zhujiang River middle and upper reaches tributary, giant salamander is generally all hidden and occupies between the stone gap of mountain stream, cave is positioned at below the water surface, night quiet keeping in the mouth clitter of beach, once find that prey is through out-of-date, just carry out surprise attack, because of its mouthful in tooth point but also close not only, after in prey inlet port, be difficult to run away.The giant salamander has very strong resistance to hungry ability, and raising does not take food for two, three years in refrigerant water can not die of hunger yet.Its simultaneously also can gluttony, make a hearty meal can put on weight 1/5th, during deprivation of food, the phenomenon that also there will be cannibalism, even with ovum, allay one's hunger, because the reproduction rate of these unique life habit giant salamanders of the giant salamander is not high, and because the ornamental value of the giant salamander and edibility are all large especially to the demand of the giant salamander on high especially market, therefore people begin one's study and propagate on a large scale the giant salamander artificially, and when propagating the giant salamander artificially, adopted a variety of ways to carry out artificial propagation to the giant salamander, comprising pure artificial propagation and pure imitative wild breeding, but these two kinds of propagation methods are to there being shortcoming separately, wherein the sexual development of the pure artificial propagation giant salamander is bad, pure imitative wild breeding there will be the nonsynchronous difficult problem of male and female fish.
Summary of the invention:
Technical problem to be solved by this invention is to provide a kind of and has solved the pure imitative nonsynchronous difficult problem of wild breeding male and female fish of the bad and giant salamander of pure artificial propagation sexual development, and can effectively improve the high-efficiency artificial raising technology of the giant salamander of reproduction rate.
Technical problem to be solved by this invention adopts following technical scheme to realize:
A high-efficiency artificial raising technology for the giant salamander, comprises following technical method:
1, annual mid-August the giant salamander when starting to carry out the sexual maturity stage, the giant salamander is classified according to the male and female of sexually matured different situations and the giant salamander, during classification, the male and female giant salamander is separated under the condition of complete lucifuge, then observe Dang Paizhi hole, the stagnant hole of doll's steck and be aubergine and sound out the people in a given scope one by one in order to break a criminal case when Shi Paizhi hole, stagnant hole can be decontroled naturally and can inject the pin of promoting the sexual maturity with hand;
2, the allotment parenteral solution of promoting the sexual maturity: by HCG:10000miu/ml, No. 2 10 micrograms of ovulation induction/and the mutual mixing and stirring of physiological saline of 2 milliliter 0.9% make the parenteral solution of promoting the sexual maturity;
3, after the parenteral solution of promoting the sexual maturity has been modulated, to carry a few days ago and inject to meeting the male giant salamander of injection requirement the pin of promoting the sexual maturity, the metering of injection is the parenteral solution of promoting the sexual maturity of per kilogram giant salamander injection 0.4ml; Both before injection, needed the giant salamander to weigh, and according to weight, the giant salamander had been injected, the female giant salamander is injected in time in the situation that satisfying condition;
4, injection is promoted the sexual maturity after pin, and female crying of a child fish must manually be extracted fertilized egg out timely within 90 hours, was placed in dry vessel;
5, then with hand, extrude the sperm of milter, and use microexamination sperm viability, select the sperm of enough vigor, the sperm of the male giant salamander is evenly dropped on the fertilized egg of the female giant salamander of prior extraction;
6, when dripping off sperm, after five minutes, add 0.9% physiological saline by the complete submergence of fertilized egg, within after submergence 15 minutes, complete fertilization;
7, repeatedly with clear water, rinse fertilized egg 5 times after being fertilized, to rinse out unnecessary sperm completely;
8, fertilized egg is placed in hatchery after rinsing well, after 24 hours, checks fertilized egg, if having go mouldy, floating, variable color, shrivelled fertilized egg will clear up in time;
9, after cleaning, about 40 days, can hatch.
Another technical essential is that above all hatching operations all require under the condition of 15-20 ℃ and will under the condition of complete lucifuge, complete.
In the whole artificial breeding stage, not only utilized pure artificial breeding technology colleague also pure imitated wild breeding technology to be combined, after carrying out mating, can directly fertilized egg be placed in the hatchery in field, do not need to carry out special hatching facility, and the mode of being arranged stagnant hole and being adopted hand to touch by observation when manually differentiating giant salamander maturity can be put forward high-resolution accuracy rate, than the mode of traditional single observation, more can observe accurately the maturity of the giant salamander, and first fertilized egg is placed in dry vessel and then sperm is evenly dropped on fertilized egg in carrying out the process that sperm and fertilized egg mutually combine, then be immersed in timely in 0.9% physiological saline, thereby this survival rate that can not only improve the giant salamander also can be carried out the hatching quality that sterilization improves the giant salamander to fertilized egg and sperm, the giant salamander that not only breeds out by high-efficiency artificial raising technology is like this stronger to the adaptive capacity of the life condition in field, resistance against diseases is also better, and to compare the time of breeding short with other technology, survival rate is high, the giant salamander bred and cultivates the promotion of having played matter.
The invention has the beneficial effects as follows: pure artificial propagation and pure imitative wild breeding are combined, solve the bad and pure imitative nonsynchronous difficult problem of wild breeding male and female fish of pure artificial propagation sexual development, adopted the method that both combine to improve reproductive efficiency and the quality of the giant salamander.
Embodiment:
For technological means, creation characteristic that the present invention is realized, reach object and effect is easy to understand, below in conjunction with concrete example, further set forth the present invention.
Specific embodiment:
1, the allotment parenteral solution of promoting the sexual maturity: by HCG:10000miu/ml, No. 2 10 micrograms of ovulation induction/and the mutual mixing and stirring of physiological saline of 2 milliliter 0.9% make the parenteral solution of promoting the sexual maturity;
2, then annual mid-August the giant salamander when starting to carry out the sexual maturity stage, the giant salamander is classified according to the male and female of sexually matured different situations and the giant salamander, during classification, the male and female giant salamander is separated under the condition of complete lucifuge, then observe Dang Paizhi hole, the stagnant hole of doll's steck and be aubergine and sound out the people in a given scope one by one in order to break a criminal case when Shi Paizhi hole, stagnant hole can be decontroled naturally and can inject the pin of promoting the sexual maturity with hand;
3, after the parenteral solution of promoting the sexual maturity has been modulated, to carry a few days ago and inject to meeting the male giant salamander of injection requirement the pin of promoting the sexual maturity, the metering of injection is the parenteral solution of promoting the sexual maturity of per kilogram giant salamander injection 0.4ml; Both before injection, needed the giant salamander to weigh, and according to weight, the giant salamander had been injected, the female giant salamander is injected in time in the situation that satisfying condition;
4, injection is promoted the sexual maturity after pin, and female crying of a child fish must manually be extracted fertilized egg out timely within 90 hours, was placed in dry vessel;
5, then with hand, extrude the sperm of milter, and use microexamination sperm viability, select the sperm of enough vigor, the sperm of the male giant salamander is evenly dropped on the fertilized egg of the female giant salamander of prior extraction;
6, when dripping off sperm, after five minutes, add 0.9% physiological saline by the complete submergence of fertilized egg, within after submergence 15 minutes, complete fertilization;
7, repeatedly with clear water, rinse fertilized egg 5 times after being fertilized, to rinse out unnecessary sperm completely;
8, fertilized egg is placed in hatchery after rinsing well, after 24 hours, checks fertilized egg, if having go mouldy, floating, variable color, shrivelled fertilized egg will clear up in time;
9, after cleaning, about 40 days, can hatch.
Following table is for breeding time experimental data contrast table:
| ? | Repoductive time | Breeding quality (the 100 tail amount of surviving) | Development condition |
| High-efficiency artificial raising technology | About 40 days | More than 95 | Good |
| Pure imitative wild breeding | About 50 days | 75-80 | Good |
| Pure artificial propagation | About 45 days | 85-95 | Good |
Following table is for breeding quality experimental data contrast table:
| ? | Every 100 form the ratio of living | Field condition becomes viability | Health degree |
| High-efficiency artificial raising technology | 95% | By force | Excellent |
| Pure imitative wild breeding | 76% | By force | Good |
| Pure artificial propagation | 85% | Good | Good |
More than show and described basic principle of the present invention and principal character and advantage of the present invention.The technical staff of the industry should understand; the present invention is not restricted to the described embodiments; that in above-described embodiment and specification, describes just illustrates principle of the present invention; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements all fall in the claimed scope of the invention.The claimed scope of the present invention is defined by appending claims and equivalent thereof.
Claims (2)
1. a high-efficiency artificial raising technology for the giant salamander, is characterized in that: comprise following technical method:
(1), annual mid-August the giant salamander when starting to carry out the sexual maturity stage, the giant salamander is classified according to the male and female of sexually matured different situations and the giant salamander, during classification, the male and female giant salamander is separated under the condition of complete lucifuge, then observe Dang Paizhi hole, the stagnant hole of doll's steck and be aubergine and sound out the people in a given scope one by one in order to break a criminal case when Shi Paizhi hole, stagnant hole can be decontroled naturally and can inject the pin of promoting the sexual maturity with hand;
(2), the allotment parenteral solution of promoting the sexual maturity: by HCG:10000miu/ml, No. 2 10 micrograms of ovulation induction/and the mutual mixing and stirring of physiological saline of 2 milliliter 0.9% make the parenteral solution of promoting the sexual maturity;
(3), after the parenteral solution of promoting the sexual maturity has been modulated, carry a few days ago and inject to meeting the male giant salamander that injection requires the pin of promoting the sexual maturity, the metering of injection is the parenteral solution of promoting the sexual maturity that the per kilogram giant salamander is injected 0.4ml; Both before injection, needed the giant salamander to weigh, and according to weight, the giant salamander had been injected, the female giant salamander is injected in time in the situation that satisfying condition;
(4), injection promote the sexual maturity after pin, female crying of a child fish must manually be extracted fertilized egg out timely within 90 hours, was placed in dry vessel;
(5), then with hand, extrude the sperm of milter, and use microexamination sperm viability, select the sperm of enough vigor, the sperm of the male giant salamander is evenly dropped on the fertilized egg of the female giant salamander of prior extraction;
(6), when dripping off sperm, after five minutes, add 0.9% physiological saline by the complete submergence of fertilized egg, within after submergence 15 minutes, complete fertilization;
(7), repeatedly with clear water, rinse fertilized egg 5 times after being fertilized, to rinse out unnecessary sperm completely;
(8), fertilized egg is placed in hatchery after rinsing well, after 24 hours, check fertilized egg, if having go mouldy, floating, variable color, shrivelled fertilized egg will clear up in time;
(9), after cleaning, about 40 days, can hatch.
2. the high-efficiency artificial raising technology of a kind of giant salamander according to claim 1, is characterized in that: all breeding operations all require under the condition of 15-20 ℃ and will under the condition of complete lucifuge, complete.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410155454.6A CN103918602B (en) | 2014-04-17 | 2014-04-17 | A kind of high-efficiency artificial mating system of Megalobatrachus japonicus daoidianuas (Blanchard) |
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| CN201410155454.6A CN103918602B (en) | 2014-04-17 | 2014-04-17 | A kind of high-efficiency artificial mating system of Megalobatrachus japonicus daoidianuas (Blanchard) |
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| CN103918602B CN103918602B (en) | 2016-08-24 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104430169A (en) * | 2014-12-12 | 2015-03-25 | 永新县水产站 | Method for promoting gonad development and increasing birth rate, fertility rate and incubation rate of giant salamanders |
| CN104542473A (en) * | 2014-12-31 | 2015-04-29 | 重庆市杰亚大鲵养殖有限公司 | Ecological breeding method of giant salamanders |
| CN104737977A (en) * | 2015-03-10 | 2015-07-01 | 湖北宜都渔洋大鲵开发有限公司 | Artificial spawning inducing and inseminating method for giant salamander |
| CN104969894A (en) * | 2015-08-10 | 2015-10-14 | 苏州市相城区阳澄湖镇剑美水产生态养殖专业合作社 | Artificial breeding method of giant salamanders |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1353927A (en) * | 2001-12-18 | 2002-06-19 | 王国兴 | Artificial reproduction method of large salamander |
| CN1613302A (en) * | 2003-11-06 | 2005-05-11 | 斗门金鲵野生动物养殖有限公司 | Method for synchronously developing gonads of salamander parents |
| CN101720720A (en) * | 2010-01-04 | 2010-06-09 | 岳西县野山土特产贸易有限公司 | Method for artificial induction breeding and biomimetic breeding of andrias davidiamus |
-
2014
- 2014-04-17 CN CN201410155454.6A patent/CN103918602B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1353927A (en) * | 2001-12-18 | 2002-06-19 | 王国兴 | Artificial reproduction method of large salamander |
| CN1613302A (en) * | 2003-11-06 | 2005-05-11 | 斗门金鲵野生动物养殖有限公司 | Method for synchronously developing gonads of salamander parents |
| CN101720720A (en) * | 2010-01-04 | 2010-06-09 | 岳西县野山土特产贸易有限公司 | Method for artificial induction breeding and biomimetic breeding of andrias davidiamus |
Non-Patent Citations (5)
| Title |
|---|
| 刘鉴毅 等: "中国大鲵养殖繁育技术的探讨", 《经济动物学报》 * |
| 施学文: "大鲵人工繁殖技术初步研究", 《福建水产》 * |
| 施学文: "大鲵人工繁殖技术初步研究", 《福建水产》, vol. 33, no. 5, 31 December 2011 (2011-12-31), pages 48 - 50 * |
| 李海珠 等: "中国大鲵人工授精方法的效果比较", 《广东农业科学》 * |
| 肖汉兵 等: "池养大鲵的人工催产研究", 《水生生物学报》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104430169A (en) * | 2014-12-12 | 2015-03-25 | 永新县水产站 | Method for promoting gonad development and increasing birth rate, fertility rate and incubation rate of giant salamanders |
| CN104542473A (en) * | 2014-12-31 | 2015-04-29 | 重庆市杰亚大鲵养殖有限公司 | Ecological breeding method of giant salamanders |
| CN104737977A (en) * | 2015-03-10 | 2015-07-01 | 湖北宜都渔洋大鲵开发有限公司 | Artificial spawning inducing and inseminating method for giant salamander |
| CN104969894A (en) * | 2015-08-10 | 2015-10-14 | 苏州市相城区阳澄湖镇剑美水产生态养殖专业合作社 | Artificial breeding method of giant salamanders |
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