CN110200725A - A kind of mandarin sturgeon hurtless measure semen collection method and device - Google Patents

A kind of mandarin sturgeon hurtless measure semen collection method and device Download PDF

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Publication number
CN110200725A
CN110200725A CN201910576886.7A CN201910576886A CN110200725A CN 110200725 A CN110200725 A CN 110200725A CN 201910576886 A CN201910576886 A CN 201910576886A CN 110200725 A CN110200725 A CN 110200725A
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CN
China
Prior art keywords
semen collection
mandarin sturgeon
fish body
gonopore
smart bottle
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Pending
Application number
CN201910576886.7A
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Chinese (zh)
Inventor
张德志
陈磊
管敏
杨元金
高勇
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Chinese Sturgeon Research Institute of China Three Gorges Corp
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Chinese Sturgeon Research Institute of China Three Gorges Corp
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Priority to CN201910576886.7A priority Critical patent/CN110200725A/en
Publication of CN110200725A publication Critical patent/CN110200725A/en
Pending legal-status Critical Current

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    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61DVETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D19/00Instruments or methods for reproduction or fertilisation
    • A61D19/02Instruments or methods for reproduction or fertilisation for artificial insemination
    • A61D19/021Apparatus for collecting seminal fluids; Artificial vaginas
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61DVETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D19/00Instruments or methods for reproduction or fertilisation
    • A61D19/02Instruments or methods for reproduction or fertilisation for artificial insemination
    • A61D19/022Containers for animal semen, e.g. pouches or vials ; Methods or apparatus for treating or handling animal semen containers, e.g. filling or closing
    • A61D19/024Tube-like containers, e.g. straws
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61DVETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D19/00Instruments or methods for reproduction or fertilisation
    • A61D19/02Instruments or methods for reproduction or fertilisation for artificial insemination
    • A61D19/022Containers for animal semen, e.g. pouches or vials ; Methods or apparatus for treating or handling animal semen containers, e.g. filling or closing
    • A61D19/025Containers for animal semen, e.g. pouches or vials ; Methods or apparatus for treating or handling animal semen containers, e.g. filling or closing with means for controlling the temperature, e.g. heating or cooling
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61DVETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D7/00Devices or methods for introducing solid, liquid, or gaseous remedies or other materials into or onto the bodies of animals

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Animal Behavior & Ethology (AREA)
  • General Health & Medical Sciences (AREA)
  • Public Health (AREA)
  • Reproductive Health (AREA)
  • Farming Of Fish And Shellfish (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The present invention relates to a kind of mandarin sturgeon hurtless measure semen collection methods, the semen collection method is that the anesthesia of mandarin sturgeon milter is placed on semen collection bed, first the water near fish body gonopore is dried with blotting paper, then silica gel catheter is inserted into fish body 10-15cm from gonopore, the position smart bottle will be deposited be placed in the 20-30cm lower than fish body gonopore, the intracorporal sperm inflow of fish is deposited in smart bottle to be flowed into until depositing aspermia in smart bottle, silica gel hose is extracted out from fish body gonopore, semen collection terminates, and fish is transferred in clear water reserviors and carries out recovery completion semen collection.The present invention can solve mandarin sturgeon milter because caused by continuous extrusion repeatedly fish body be mechanically damaged lethal problem, extremely significant improves mandarin sturgeon milter postpartum survival rate, it can most importantly prevent extraneous water from entering simultaneously and deposit smart bottle, ensure that being perfectly safe for semen collection.

Description

A kind of mandarin sturgeon hurtless measure semen collection method and device
The present invention relates to rare Endemic fish species conservation technical field, especially a kind of mandarin sturgeon hurtless measure semen collection method And device.
Background technique
Mandarin sturgeon is the migratory Chondrostei in river sea, is distributed mainly on China main stream of the Yangtze and southeastern coast, 20 Th century later, due to factors such as overfishing, hydraulic engineering construction, shipping, water pollutions, wild stocks resource is continuous Decline.Although mandarin sturgeon was listed in country-level aquatic protection animal in 1988, between 19 years of 1981-1999, in The young sturgeon recruitment stock of magnificent sturgeon still reduces 90% or so, so, it is very urgent for the protection and research work of mandarin sturgeon.
Acipenser Sinensis Institute of China Three Gorges Corporation and other Ji Jia scientific research institutions mandarin sturgeon protection and grind Study carefully aspect to do a lot of work, this is of great significance for the species conservation of mandarin sturgeon.But we are complete in development mandarin sturgeon Artificial propagation is found when working, and when carrying out semen collection to milter, can be carried out continuous extrusion repeatedly to fish body abdomen, be easy to cause Fish body body injury, so as to cause death.In consideration of it, currently need to research and develop a kind of mandarin sturgeon hurtless measure semen collection method and device, It is time saving and energy saving, it avoids causing body injury to mandarin sturgeon.
Summary of the invention
In view of the deficiency of above-mentioned existing production technology, it is an object of the invention to a kind of mandarin sturgeon hurtless measure semen collection method and Device.
The invention adopts the following technical scheme:
A kind of mandarin sturgeon hurtless measure semen collection method, the semen collection method are that the anesthesia of mandarin sturgeon milter is placed on semen collection bed, are first used Blotting paper dries the water near fish body gonopore, and silica gel catheter is then inserted into fish body 10-15cm from gonopore, will deposit smart bottle It is placed in the position of the 20-30cm lower than fish body gonopore, the intracorporal sperm inflow of fish is deposited in smart bottle until depositing in smart bottle without essence Liquid stream enters, and silica gel hose is extracted out from fish body gonopore, and semen collection terminates, and fish is transferred in clear water reserviors recover and completes to adopt Essence.
Preferably, the anesthesia procedure method specifically includes the following steps: (1) anesthetic configuration: MS-222 is dissolved Into watering can, it is made into the anesthetic that concentration is 100-120 mg/L;(2) it anaesthetizes: mandarin sturgeon milter is transferred on semen collection bed, use Wet towel fixes its head and tail portion, sprays anesthetic to its gill portion, semen collection is carried out after fish body enters narcosis.
It is further preferred that the sperm of acquisition pours into semen storing bag, every bag of 30-50ml uses rubber after being filled with pure oxygen into bag Rubber band ties, and is then inserted in black bag and is protected from light, and the penicillin of 5000 international units is added in every milliliter of sperm, inhibits bacterium numerous It grows;It is subsequently placed in insulating box, is saved using being segmented after falling temperature method cools the temperature to 2 DEG C at a slow speed.
It is further preferred that temperature-fall period is that sperm is gradually decreased to 2 DEG C by 18-20 DEG C, every 0.5h declines 1 DEG C, directly To being down to 2 DEG C.
The semen collection device that the method uses, the semen collection device includes afferent duct and deposits smart bottle, described to deposit smart bottle bottle Mouthful be equipped with cork, glass tube passes through cork and protrudes into and deposit inside smart bottle, glass tube be located at cork lateral ends and Afferent duct connection.
Preferably, outlet pore is additionally provided on the cork, escape pipe is inserted into the outlet pore of cork.
Preferably, the afferent duct is the silica gel hose of a long 30-50cm, internal diameter 6-8mm;The glass pipe range 6- 8cm outer diameter is 6-8mm;The escape pipe is the glass tube of a long 3-5cm, diameter 10-13mm, and cork is inserted into escape pipe one end In the outlet pore of bottle stopper;The cork is the cylindrical body of a basal diameter 6-10cm, high 2-3cm;It is described to deposit smart bottle For the cylindrical body vial of a bottom surface internal diameter 6.01-10.01cm, high 10-18cm, wall thickness 1.5mm.
The invention has the following advantages:
1, the present invention can solve mandarin sturgeon milter because caused by continuous extrusion repeatedly fish body be mechanically damaged lethal problem, extremely show What is write improves mandarin sturgeon milter postpartum survival rate, while can most importantly prevent extraneous water from entering and deposit smart bottle, ensure that Semen collection is perfectly safe.
2, the present invention utilizes flow by gravity principle, the intracorporal sperm importing of mandarin sturgeon milter is deposited in smart bottle, not to fish body Abdomen implements any extruding, thoroughly solves mandarin sturgeon milter in semen collection because that continuous extrusion repeatedly causes fish injury is lethal Problem, improve the postpartum survival rate of mandarin sturgeon milter extremely significantly.
3, the present invention utilizes anaesthesia technology, to anaesthetizing before milter semen collection to it, not only solves and grasps in artificial semenpicking Because fish stress revolt by force during work, the difficult problem of semen collection operation, it is thus also avoided that fish causes by force fish body injured because that stress revolt.It adopts The sperm of collection pours into semen storing bag, every bag of 30-50ml, is tied after pure oxygen is filled with into bag with rubber band, is then inserted in black bag In be protected from light, the penicillin of 5000 international units is added in every milliliter of sperm, inhibits bacterial reproduction;It is subsequently placed in insulating box, uses Falling temperature method saves after cooling the temperature to 2 DEG C at a slow speed for segmentation.
The flourish for inhibiting the microorganisms such as bacterium in sperm, extends the holding time of sperm.Avoid temperature decrease to essence The intense stimulus of liquid improves the survival rate of sperm in sperm, can extend the holding time of sperm.
4, the semen collection device used in the present invention is whole closing appliance, complete compared with complete open bowl basin in the past It solves the problems, such as to cause sperm to cancel activation of spermatozoa since water droplet is splashed into sperm entirely.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of semen collection device of the present invention;
1-afferent duct;2-glass tubes;3-escape pipes;4-corks;5-deposit smart bottle.
Specific embodiment
Combined with specific embodiments below, further details of elaboration is made to the present invention, but embodiments of the present invention are not It is confined to the range of embodiment expression.These embodiments are merely to illustrate the present invention, range and is not intended to limit the present invention.
Embodiment 1
A kind of mandarin sturgeon hurtless measure semen collection method, the semen collection method are that the anesthesia of mandarin sturgeon milter is placed on semen collection bed, are first used Blotting paper dries the water near fish body gonopore, and silica gel catheter is then inserted into fish body 10-15cm from gonopore, will deposit smart bottle It is placed in the position of the 20-30cm lower than fish body gonopore, the intracorporal sperm inflow of fish is deposited in smart bottle until depositing in smart bottle without essence Liquid stream enters, and silica gel hose is extracted out from fish body gonopore, and semen collection terminates, and fish is transferred in clear water reserviors recover and completes to adopt Essence.
Preferably, the anesthesia procedure method specifically includes the following steps: anesthetic configuration: MS-222 is dissolved to spray In pot, it is made into the anesthetic that concentration is 100-120 mg/L;Anesthesia: mandarin sturgeon milter is transferred on semen collection bed, wet towel is used Its head and tail portion are fixed, anesthetic is sprayed to its gill portion, semen collection is carried out after fish body enters narcosis.
It is further preferred that the sperm of acquisition pours into semen storing bag, every bag of 30-50ml uses rubber after being filled with pure oxygen into bag Rubber band ties, and is then inserted in black bag and is protected from light, and the penicillin of 5000 international units is added in every milliliter of sperm, inhibits bacterium numerous It grows;It is subsequently placed in insulating box, is saved using being segmented after falling temperature method cools the temperature to 2 DEG C at a slow speed.
It is further preferred that temperature-fall period is that sperm is gradually decreased to 2 DEG C by 18-20 DEG C, every 0.5h declines 1 DEG C, directly To being down to 2 DEG C.
The semen collection device that the method uses, the semen collection device includes afferent duct and deposits smart bottle, described to deposit 5 bottles of smart bottle Mouth is equipped with cork 4, and glass tube 2 is protruded into across cork 4 and deposited inside smart bottle 5, and glass tube 2 is located at 4 outside of cork One end is connect with afferent duct 1.
Preferably, outlet pore is additionally provided on the cork 4, escape pipe 3 is inserted into the outlet pore of cork.
Preferably, the afferent duct 1 is the silica gel hose of a long 30-50cm, internal diameter 6-8mm;The glass tube 2 is 6- long 8cm outer diameter is 6-8mm;The escape pipe 3 is the glass tube of a long 3-5cm, diameter 10-13mm, and the insertion of escape pipe one end is soft In the outlet pore of bowling pin plug;The cork 4 is the cylindrical body of a basal diameter 6-10cm, high 2-3cm;It is described to deposit essence Bottle 5 is the cylindrical body vial of a bottom surface internal diameter 6.01-10.01cm, high 10-18cm, wall thickness 1.5mm.
Pass through the above method, in November, 2017, before not taking any extrusion operation to mandarin sturgeon milter fish body abdomen It putting, successfully completes the semen collection work of 3 mandarin sturgeon milters, obtain sperm 115ml, milter is not affected by any mechanical damage, It is lethal due to continuous extrusion causes fish injury repeatedly in semen collection thoroughly to solve mandarin sturgeon milter up to 100% for postpartum survival rate Problem.
Embodiment 2
A kind of mandarin sturgeon hurtless measure semen collection device by afferent duct 1, glass tube 2, escape pipe 3, cork 4, is deposited smart bottle 5 and is formed;
The afferent duct 1 is the silica gel hose of a long 30-50cm, internal diameter 6-8mm;
The 6-8cm long outer diameter of glass tube 2 is 6-8mm, and 2 one end of glass tube is connect with afferent duct, and the other end is inserted into cork bottle In the glass pore of plug 4;
The escape pipe 3 is the glass tube of a long 3-5cm, diameter 10-13mm, and the outlet of cork is inserted into escape pipe one end In pore;
The cork 4 is the cylindrical body of a basal diameter 6-10cm, high 2-3cm, there is glass pore and escape pipe thereon Hole, glass pore diameter are 6.1-8.1mm, and outlet pore diameter is 10.1-13.1mm;
Described deposits the cylindrical body vial that smart bottle 5 is a bottom surface internal diameter 6.01-10.01cm, high 10-18cm, wall thickness 1.5mm.

Claims (7)

1. a kind of mandarin sturgeon hurtless measure semen collection method, which is characterized in that the semen collection method is that the anesthesia of mandarin sturgeon milter is placed on On semen collection bed, first the water near fish body gonopore is dried with blotting paper, silica gel catheter is then inserted into fish body 10- from gonopore 15cm, the position smart bottle will be deposited be placed in the 20-30cm lower than fish body gonopore, the intracorporal sperm inflow of fish deposit in smart bottle until It deposits aspermia in smart bottle to flow into, silica gel hose be extracted out from fish body gonopore, semen collection terminates, and fish is transferred in clear water reserviors and is carried out Semen collection is completed in recovery.
2. mandarin sturgeon hurtless measure semen collection method according to claim 1, it is characterised in that: the anesthesia procedure method is specific The following steps are included: the configuration of (1) anesthetic: MS-222 being dissolved in watering can, the fiber crops that concentration is 100-120 mg/L are made into Liquor-saturated dose;(2) it anaesthetizes: mandarin sturgeon milter being transferred on semen collection bed, fixes its head and tail portion with wet towel, its gill portion is sprayed Anesthetic carries out semen collection after fish body enters narcosis.
3. mandarin sturgeon hurtless measure semen collection method according to claim 2, it is characterised in that: the sperm of acquisition pours into semen storing bag In, every bag of 30-50ml is tied with rubber band after being filled with pure oxygen into bag, is then inserted in black bag and is protected from light, and every milliliter of sperm adds Enter the penicillin of 5000 international units, inhibits bacterial reproduction;Be subsequently placed in insulating box, using segmentation at a slow speed falling temperature method by temperature It is saved after being down to 2 DEG C.
4. according to claim 3 be segmented falling temperature method at a slow speed, it is characterised in that: temperature-fall period is by sperm by 18-20 DEG C 2 DEG C are gradually decreased to, every 0.5h declines 1 DEG C, until being down to 2 DEG C.
5. the semen collection device that method described in claim 1-4 any one uses, it is characterised in that: the semen collection device includes It afferent duct (1) and deposits smart bottle (5), it is characterised in that: smart bottle (5) bottleneck of depositing equipped with cork (4), wear by glass tube (2) It crosses cork (4) and protrudes into and deposit that smart bottle (5) is internal, and glass tube (2) is located at cork (4) lateral ends and afferent duct (1) company It connects.
6. device according to claim 5, it is characterised in that: be additionally provided with outlet pore, outlet on the cork (4) In the outlet pore for managing (3) insertion cork.
7. device according to claim 5, it is characterised in that: the afferent duct (1) is a long 30-50cm, internal diameter 6-8mm Silica gel hose;The long 6-8cm outer diameter of glass tube (2) is 6-8mm;The escape pipe (3) is a long 3-5cm, diameter The glass tube of 10-13mm, escape pipe one end are inserted into the outlet pore of cork;The cork (4) is a bottom surface The cylindrical body of diameter 6-10cm, high 2-3cm;Described deposit smart bottle (5) are a bottom surface internal diameter 6.01-10.01cm, high 10-18cm Cylindrical body vial, wall thickness 1.5mm.
CN201910576886.7A 2019-06-28 2019-06-28 A kind of mandarin sturgeon hurtless measure semen collection method and device Pending CN110200725A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113367122A (en) * 2021-06-01 2021-09-10 水利部中国科学院水工程生态研究所 Chinese sturgeon F2Sperm ultra-low temperature freezing preservation method

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CN104145869A (en) * 2014-08-27 2014-11-19 四川省农业科学院水产研究所 Artificial breeding method for acipenser dabryanus
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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113367122A (en) * 2021-06-01 2021-09-10 水利部中国科学院水工程生态研究所 Chinese sturgeon F2Sperm ultra-low temperature freezing preservation method

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Application publication date: 20190906