CN110200725A - A kind of mandarin sturgeon hurtless measure semen collection method and device - Google Patents
A kind of mandarin sturgeon hurtless measure semen collection method and device Download PDFInfo
- Publication number
- CN110200725A CN110200725A CN201910576886.7A CN201910576886A CN110200725A CN 110200725 A CN110200725 A CN 110200725A CN 201910576886 A CN201910576886 A CN 201910576886A CN 110200725 A CN110200725 A CN 110200725A
- Authority
- CN
- China
- Prior art keywords
- semen collection
- mandarin sturgeon
- fish body
- gonopore
- smart bottle
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D19/00—Instruments or methods for reproduction or fertilisation
- A61D19/02—Instruments or methods for reproduction or fertilisation for artificial insemination
- A61D19/021—Apparatus for collecting seminal fluids; Artificial vaginas
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D19/00—Instruments or methods for reproduction or fertilisation
- A61D19/02—Instruments or methods for reproduction or fertilisation for artificial insemination
- A61D19/022—Containers for animal semen, e.g. pouches or vials ; Methods or apparatus for treating or handling animal semen containers, e.g. filling or closing
- A61D19/024—Tube-like containers, e.g. straws
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D19/00—Instruments or methods for reproduction or fertilisation
- A61D19/02—Instruments or methods for reproduction or fertilisation for artificial insemination
- A61D19/022—Containers for animal semen, e.g. pouches or vials ; Methods or apparatus for treating or handling animal semen containers, e.g. filling or closing
- A61D19/025—Containers for animal semen, e.g. pouches or vials ; Methods or apparatus for treating or handling animal semen containers, e.g. filling or closing with means for controlling the temperature, e.g. heating or cooling
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D7/00—Devices or methods for introducing solid, liquid, or gaseous remedies or other materials into or onto the bodies of animals
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Reproductive Health (AREA)
- Farming Of Fish And Shellfish (AREA)
- Peptides Or Proteins (AREA)
Abstract
The present invention relates to a kind of mandarin sturgeon hurtless measure semen collection methods, the semen collection method is that the anesthesia of mandarin sturgeon milter is placed on semen collection bed, first the water near fish body gonopore is dried with blotting paper, then silica gel catheter is inserted into fish body 10-15cm from gonopore, the position smart bottle will be deposited be placed in the 20-30cm lower than fish body gonopore, the intracorporal sperm inflow of fish is deposited in smart bottle to be flowed into until depositing aspermia in smart bottle, silica gel hose is extracted out from fish body gonopore, semen collection terminates, and fish is transferred in clear water reserviors and carries out recovery completion semen collection.The present invention can solve mandarin sturgeon milter because caused by continuous extrusion repeatedly fish body be mechanically damaged lethal problem, extremely significant improves mandarin sturgeon milter postpartum survival rate, it can most importantly prevent extraneous water from entering simultaneously and deposit smart bottle, ensure that being perfectly safe for semen collection.
Description
The present invention relates to rare Endemic fish species conservation technical field, especially a kind of mandarin sturgeon hurtless measure semen collection method
And device.
Background technique
Mandarin sturgeon is the migratory Chondrostei in river sea, is distributed mainly on China main stream of the Yangtze and southeastern coast, 20
Th century later, due to factors such as overfishing, hydraulic engineering construction, shipping, water pollutions, wild stocks resource is continuous
Decline.Although mandarin sturgeon was listed in country-level aquatic protection animal in 1988, between 19 years of 1981-1999, in
The young sturgeon recruitment stock of magnificent sturgeon still reduces 90% or so, so, it is very urgent for the protection and research work of mandarin sturgeon.
Acipenser Sinensis Institute of China Three Gorges Corporation and other Ji Jia scientific research institutions mandarin sturgeon protection and grind
Study carefully aspect to do a lot of work, this is of great significance for the species conservation of mandarin sturgeon.But we are complete in development mandarin sturgeon
Artificial propagation is found when working, and when carrying out semen collection to milter, can be carried out continuous extrusion repeatedly to fish body abdomen, be easy to cause
Fish body body injury, so as to cause death.In consideration of it, currently need to research and develop a kind of mandarin sturgeon hurtless measure semen collection method and device,
It is time saving and energy saving, it avoids causing body injury to mandarin sturgeon.
Summary of the invention
In view of the deficiency of above-mentioned existing production technology, it is an object of the invention to a kind of mandarin sturgeon hurtless measure semen collection method and
Device.
The invention adopts the following technical scheme:
A kind of mandarin sturgeon hurtless measure semen collection method, the semen collection method are that the anesthesia of mandarin sturgeon milter is placed on semen collection bed, are first used
Blotting paper dries the water near fish body gonopore, and silica gel catheter is then inserted into fish body 10-15cm from gonopore, will deposit smart bottle
It is placed in the position of the 20-30cm lower than fish body gonopore, the intracorporal sperm inflow of fish is deposited in smart bottle until depositing in smart bottle without essence
Liquid stream enters, and silica gel hose is extracted out from fish body gonopore, and semen collection terminates, and fish is transferred in clear water reserviors recover and completes to adopt
Essence.
Preferably, the anesthesia procedure method specifically includes the following steps: (1) anesthetic configuration: MS-222 is dissolved
Into watering can, it is made into the anesthetic that concentration is 100-120 mg/L;(2) it anaesthetizes: mandarin sturgeon milter is transferred on semen collection bed, use
Wet towel fixes its head and tail portion, sprays anesthetic to its gill portion, semen collection is carried out after fish body enters narcosis.
It is further preferred that the sperm of acquisition pours into semen storing bag, every bag of 30-50ml uses rubber after being filled with pure oxygen into bag
Rubber band ties, and is then inserted in black bag and is protected from light, and the penicillin of 5000 international units is added in every milliliter of sperm, inhibits bacterium numerous
It grows;It is subsequently placed in insulating box, is saved using being segmented after falling temperature method cools the temperature to 2 DEG C at a slow speed.
It is further preferred that temperature-fall period is that sperm is gradually decreased to 2 DEG C by 18-20 DEG C, every 0.5h declines 1 DEG C, directly
To being down to 2 DEG C.
The semen collection device that the method uses, the semen collection device includes afferent duct and deposits smart bottle, described to deposit smart bottle bottle
Mouthful be equipped with cork, glass tube passes through cork and protrudes into and deposit inside smart bottle, glass tube be located at cork lateral ends and
Afferent duct connection.
Preferably, outlet pore is additionally provided on the cork, escape pipe is inserted into the outlet pore of cork.
Preferably, the afferent duct is the silica gel hose of a long 30-50cm, internal diameter 6-8mm;The glass pipe range 6-
8cm outer diameter is 6-8mm;The escape pipe is the glass tube of a long 3-5cm, diameter 10-13mm, and cork is inserted into escape pipe one end
In the outlet pore of bottle stopper;The cork is the cylindrical body of a basal diameter 6-10cm, high 2-3cm;It is described to deposit smart bottle
For the cylindrical body vial of a bottom surface internal diameter 6.01-10.01cm, high 10-18cm, wall thickness 1.5mm.
The invention has the following advantages:
1, the present invention can solve mandarin sturgeon milter because caused by continuous extrusion repeatedly fish body be mechanically damaged lethal problem, extremely show
What is write improves mandarin sturgeon milter postpartum survival rate, while can most importantly prevent extraneous water from entering and deposit smart bottle, ensure that
Semen collection is perfectly safe.
2, the present invention utilizes flow by gravity principle, the intracorporal sperm importing of mandarin sturgeon milter is deposited in smart bottle, not to fish body
Abdomen implements any extruding, thoroughly solves mandarin sturgeon milter in semen collection because that continuous extrusion repeatedly causes fish injury is lethal
Problem, improve the postpartum survival rate of mandarin sturgeon milter extremely significantly.
3, the present invention utilizes anaesthesia technology, to anaesthetizing before milter semen collection to it, not only solves and grasps in artificial semenpicking
Because fish stress revolt by force during work, the difficult problem of semen collection operation, it is thus also avoided that fish causes by force fish body injured because that stress revolt.It adopts
The sperm of collection pours into semen storing bag, every bag of 30-50ml, is tied after pure oxygen is filled with into bag with rubber band, is then inserted in black bag
In be protected from light, the penicillin of 5000 international units is added in every milliliter of sperm, inhibits bacterial reproduction;It is subsequently placed in insulating box, uses
Falling temperature method saves after cooling the temperature to 2 DEG C at a slow speed for segmentation.
The flourish for inhibiting the microorganisms such as bacterium in sperm, extends the holding time of sperm.Avoid temperature decrease to essence
The intense stimulus of liquid improves the survival rate of sperm in sperm, can extend the holding time of sperm.
4, the semen collection device used in the present invention is whole closing appliance, complete compared with complete open bowl basin in the past
It solves the problems, such as to cause sperm to cancel activation of spermatozoa since water droplet is splashed into sperm entirely.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of semen collection device of the present invention;
1-afferent duct;2-glass tubes;3-escape pipes;4-corks;5-deposit smart bottle.
Specific embodiment
Combined with specific embodiments below, further details of elaboration is made to the present invention, but embodiments of the present invention are not
It is confined to the range of embodiment expression.These embodiments are merely to illustrate the present invention, range and is not intended to limit the present invention.
Embodiment 1
A kind of mandarin sturgeon hurtless measure semen collection method, the semen collection method are that the anesthesia of mandarin sturgeon milter is placed on semen collection bed, are first used
Blotting paper dries the water near fish body gonopore, and silica gel catheter is then inserted into fish body 10-15cm from gonopore, will deposit smart bottle
It is placed in the position of the 20-30cm lower than fish body gonopore, the intracorporal sperm inflow of fish is deposited in smart bottle until depositing in smart bottle without essence
Liquid stream enters, and silica gel hose is extracted out from fish body gonopore, and semen collection terminates, and fish is transferred in clear water reserviors recover and completes to adopt
Essence.
Preferably, the anesthesia procedure method specifically includes the following steps: anesthetic configuration: MS-222 is dissolved to spray
In pot, it is made into the anesthetic that concentration is 100-120 mg/L;Anesthesia: mandarin sturgeon milter is transferred on semen collection bed, wet towel is used
Its head and tail portion are fixed, anesthetic is sprayed to its gill portion, semen collection is carried out after fish body enters narcosis.
It is further preferred that the sperm of acquisition pours into semen storing bag, every bag of 30-50ml uses rubber after being filled with pure oxygen into bag
Rubber band ties, and is then inserted in black bag and is protected from light, and the penicillin of 5000 international units is added in every milliliter of sperm, inhibits bacterium numerous
It grows;It is subsequently placed in insulating box, is saved using being segmented after falling temperature method cools the temperature to 2 DEG C at a slow speed.
It is further preferred that temperature-fall period is that sperm is gradually decreased to 2 DEG C by 18-20 DEG C, every 0.5h declines 1 DEG C, directly
To being down to 2 DEG C.
The semen collection device that the method uses, the semen collection device includes afferent duct and deposits smart bottle, described to deposit 5 bottles of smart bottle
Mouth is equipped with cork 4, and glass tube 2 is protruded into across cork 4 and deposited inside smart bottle 5, and glass tube 2 is located at 4 outside of cork
One end is connect with afferent duct 1.
Preferably, outlet pore is additionally provided on the cork 4, escape pipe 3 is inserted into the outlet pore of cork.
Preferably, the afferent duct 1 is the silica gel hose of a long 30-50cm, internal diameter 6-8mm;The glass tube 2 is 6- long
8cm outer diameter is 6-8mm;The escape pipe 3 is the glass tube of a long 3-5cm, diameter 10-13mm, and the insertion of escape pipe one end is soft
In the outlet pore of bowling pin plug;The cork 4 is the cylindrical body of a basal diameter 6-10cm, high 2-3cm;It is described to deposit essence
Bottle 5 is the cylindrical body vial of a bottom surface internal diameter 6.01-10.01cm, high 10-18cm, wall thickness 1.5mm.
Pass through the above method, in November, 2017, before not taking any extrusion operation to mandarin sturgeon milter fish body abdomen
It putting, successfully completes the semen collection work of 3 mandarin sturgeon milters, obtain sperm 115ml, milter is not affected by any mechanical damage,
It is lethal due to continuous extrusion causes fish injury repeatedly in semen collection thoroughly to solve mandarin sturgeon milter up to 100% for postpartum survival rate
Problem.
Embodiment 2
A kind of mandarin sturgeon hurtless measure semen collection device by afferent duct 1, glass tube 2, escape pipe 3, cork 4, is deposited smart bottle 5 and is formed;
The afferent duct 1 is the silica gel hose of a long 30-50cm, internal diameter 6-8mm;
The 6-8cm long outer diameter of glass tube 2 is 6-8mm, and 2 one end of glass tube is connect with afferent duct, and the other end is inserted into cork bottle
In the glass pore of plug 4;
The escape pipe 3 is the glass tube of a long 3-5cm, diameter 10-13mm, and the outlet of cork is inserted into escape pipe one end
In pore;
The cork 4 is the cylindrical body of a basal diameter 6-10cm, high 2-3cm, there is glass pore and escape pipe thereon
Hole, glass pore diameter are 6.1-8.1mm, and outlet pore diameter is 10.1-13.1mm;
Described deposits the cylindrical body vial that smart bottle 5 is a bottom surface internal diameter 6.01-10.01cm, high 10-18cm, wall thickness 1.5mm.
Claims (7)
1. a kind of mandarin sturgeon hurtless measure semen collection method, which is characterized in that the semen collection method is that the anesthesia of mandarin sturgeon milter is placed on
On semen collection bed, first the water near fish body gonopore is dried with blotting paper, silica gel catheter is then inserted into fish body 10- from gonopore
15cm, the position smart bottle will be deposited be placed in the 20-30cm lower than fish body gonopore, the intracorporal sperm inflow of fish deposit in smart bottle until
It deposits aspermia in smart bottle to flow into, silica gel hose be extracted out from fish body gonopore, semen collection terminates, and fish is transferred in clear water reserviors and is carried out
Semen collection is completed in recovery.
2. mandarin sturgeon hurtless measure semen collection method according to claim 1, it is characterised in that: the anesthesia procedure method is specific
The following steps are included: the configuration of (1) anesthetic: MS-222 being dissolved in watering can, the fiber crops that concentration is 100-120 mg/L are made into
Liquor-saturated dose;(2) it anaesthetizes: mandarin sturgeon milter being transferred on semen collection bed, fixes its head and tail portion with wet towel, its gill portion is sprayed
Anesthetic carries out semen collection after fish body enters narcosis.
3. mandarin sturgeon hurtless measure semen collection method according to claim 2, it is characterised in that: the sperm of acquisition pours into semen storing bag
In, every bag of 30-50ml is tied with rubber band after being filled with pure oxygen into bag, is then inserted in black bag and is protected from light, and every milliliter of sperm adds
Enter the penicillin of 5000 international units, inhibits bacterial reproduction;Be subsequently placed in insulating box, using segmentation at a slow speed falling temperature method by temperature
It is saved after being down to 2 DEG C.
4. according to claim 3 be segmented falling temperature method at a slow speed, it is characterised in that: temperature-fall period is by sperm by 18-20 DEG C
2 DEG C are gradually decreased to, every 0.5h declines 1 DEG C, until being down to 2 DEG C.
5. the semen collection device that method described in claim 1-4 any one uses, it is characterised in that: the semen collection device includes
It afferent duct (1) and deposits smart bottle (5), it is characterised in that: smart bottle (5) bottleneck of depositing equipped with cork (4), wear by glass tube (2)
It crosses cork (4) and protrudes into and deposit that smart bottle (5) is internal, and glass tube (2) is located at cork (4) lateral ends and afferent duct (1) company
It connects.
6. device according to claim 5, it is characterised in that: be additionally provided with outlet pore, outlet on the cork (4)
In the outlet pore for managing (3) insertion cork.
7. device according to claim 5, it is characterised in that: the afferent duct (1) is a long 30-50cm, internal diameter 6-8mm
Silica gel hose;The long 6-8cm outer diameter of glass tube (2) is 6-8mm;The escape pipe (3) is a long 3-5cm, diameter
The glass tube of 10-13mm, escape pipe one end are inserted into the outlet pore of cork;The cork (4) is a bottom surface
The cylindrical body of diameter 6-10cm, high 2-3cm;Described deposit smart bottle (5) are a bottom surface internal diameter 6.01-10.01cm, high 10-18cm
Cylindrical body vial, wall thickness 1.5mm.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201910576886.7A CN110200725A (en) | 2019-06-28 | 2019-06-28 | A kind of mandarin sturgeon hurtless measure semen collection method and device |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201910576886.7A CN110200725A (en) | 2019-06-28 | 2019-06-28 | A kind of mandarin sturgeon hurtless measure semen collection method and device |
Publications (1)
Publication Number | Publication Date |
---|---|
CN110200725A true CN110200725A (en) | 2019-09-06 |
Family
ID=67795387
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201910576886.7A Pending CN110200725A (en) | 2019-06-28 | 2019-06-28 | A kind of mandarin sturgeon hurtless measure semen collection method and device |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN110200725A (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113367122A (en) * | 2021-06-01 | 2021-09-10 | 水利部中国科学院水工程生态研究所 | Chinese sturgeon F2Sperm ultra-low temperature freezing preservation method |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20040039248A1 (en) * | 2000-08-11 | 2004-02-26 | Gerard Vayer | Animal device for collecting an animal's seminal fluid |
CN101884322A (en) * | 2010-07-20 | 2010-11-17 | 中国水产科学研究院黄海水产研究所 | Verasper moseri sperm cryopreservation method |
CN104145869A (en) * | 2014-08-27 | 2014-11-19 | 四川省农业科学院水产研究所 | Artificial breeding method for acipenser dabryanus |
CN104997520A (en) * | 2015-06-05 | 2015-10-28 | 何以安 | Blood collection apparatus |
CN207741954U (en) * | 2017-11-29 | 2018-08-17 | 上海市计划生育科学研究所 | A kind of experiment fish sperm collecting apparatus |
-
2019
- 2019-06-28 CN CN201910576886.7A patent/CN110200725A/en active Pending
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20040039248A1 (en) * | 2000-08-11 | 2004-02-26 | Gerard Vayer | Animal device for collecting an animal's seminal fluid |
CN101884322A (en) * | 2010-07-20 | 2010-11-17 | 中国水产科学研究院黄海水产研究所 | Verasper moseri sperm cryopreservation method |
CN104145869A (en) * | 2014-08-27 | 2014-11-19 | 四川省农业科学院水产研究所 | Artificial breeding method for acipenser dabryanus |
CN104997520A (en) * | 2015-06-05 | 2015-10-28 | 何以安 | Blood collection apparatus |
CN207741954U (en) * | 2017-11-29 | 2018-08-17 | 上海市计划生育科学研究所 | A kind of experiment fish sperm collecting apparatus |
Non-Patent Citations (2)
Title |
---|
无: "鲟鱼人工繁殖技术-人工催产与授精", 《HTTPS://WWW.HRFRI.AC.CN/INFO/1341/2767_1.HTM》 * |
权凯,李君: "《肉羊良种利用与繁殖技术一本通》", 30 June 2018, 中国科学技术出版社 * |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113367122A (en) * | 2021-06-01 | 2021-09-10 | 水利部中国科学院水工程生态研究所 | Chinese sturgeon F2Sperm ultra-low temperature freezing preservation method |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN110200725A (en) | A kind of mandarin sturgeon hurtless measure semen collection method and device | |
CN106577374A (en) | Domesticating method for wild euchiloglanis kishinouyei | |
CN104342401B (en) | It is adipose cell to promote fibroblast transdifferentiation using the combination of cytokines for determining | |
CN106689001A (en) | Self-circulation type prawn breeding method | |
CN103952369B (en) | One strain Ctenopharyngodon idellus Air Bladder pseudosciaenae seu Acipenser epithelioid cell system and application thereof | |
CN206365360U (en) | A kind of green stone Pa Select-Committee Ecologies raise and train pond | |
CN102763623A (en) | Culture pond for marketable giant salamanders | |
CN205143257U (en) | Bubble drives fish fish sucking pump | |
CN205390029U (en) | A surface of water foam clearing device for fish pond | |
CN102696510B (en) | Method for culturing pampus argenteus larval and young fishes with running water | |
Sonstegard et al. | Epidemiological model for passive transfer of IPNV by homeotherms | |
CN204482705U (en) | A kind of adjustable hen house feedwater and medicament blending device | |
CN210796459U (en) | Bacterium inspection sampling device | |
CN205250145U (en) | Improve equipment of edgy fish live body transportation survival rate | |
CN107714053A (en) | A kind of fixed blood-taking device of panda wrist | |
CN107646760A (en) | A kind of cultural method of pomfret parr | |
CN204132174U (en) | A kind of workbench for loach injection | |
US3057783A (en) | Infectious bovine rhinotracheitis vaccine and method of preparing it | |
CN205213912U (en) | False bait with wing | |
CN205337259U (en) | Water oxygenation device | |
CN204762826U (en) | Sea horse hapteron | |
CN206486529U (en) | A kind of sperm swims catcher | |
CN204305869U (en) | A kind of coil oxygen cathete for three-dimensional culture | |
CN203506927U (en) | Veterinary drenching device for pets | |
CN201320292Y (en) | Insemination-bag constant temperature bottle |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
RJ01 | Rejection of invention patent application after publication | ||
RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190906 |