CN104145869A - Artificial breeding method for acipenser dabryanus - Google Patents
Artificial breeding method for acipenser dabryanus Download PDFInfo
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- CN104145869A CN104145869A CN201410428801.8A CN201410428801A CN104145869A CN 104145869 A CN104145869 A CN 104145869A CN 201410428801 A CN201410428801 A CN 201410428801A CN 104145869 A CN104145869 A CN 104145869A
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- acipenser dabryanus
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention relates to the technical field of aquaculture, in particular to an artificial breeding method for acipenser dabryanus. Oxytocin is injected into female acipenser dabryanus two times, wherein the interval between two times of injection is 12 hours, and the injection amount of the first time accounts for 1/3 to 1/2 of the total dose. The one-time injection method is adopted for male acipenser dabryanus, wherein injection of the male acipenser dabryanus is synchronous with the first time of injection of the female acipenser dabryanus, and the dose of the male acipenser dabryanus is half that of the female acipenser dabryanus. Fertilization is conducted according to a half-dry method, water, eggs and seminal fluid are mixed according to the proportion of 75:50:1, and the mixture is stirred for 2 minutes to 3 minutes. A complete acipenser dabryanus artificial breeding technique process is provided, and technical support is provided for large-scale artificial breeding; due to the adoption of the method, the hatchability of acipenser dabryanus can reach 85% or higher at most, and restrict factors in the acipenser dabryanus artificial breeding process are eliminated.
Description
Technical field
The present invention relates to technical field of aquaculture, is the artificial fecundation method of a kind of acipenser dabryanus specifically.
Background technology
Acipenser dabryanus is the distinctive flagship species in China Yangtze river basin and Important Economic fish, and it is bred with wild stocks amplification and concerns China's characteristic aquatic products industry development, aquatic ecosystem is healthy and important peculiar Fish Germplasm Resources safety.Yet, because this basin water ecosystem is subject to the severe jamming of mankind's activity (as power station exploitation, water pollution and illegally fish for etc.), cause acipenser dabryanus population scale to reduce, even in Critical Condition.The severe situation changing for its population, we have carried out serial ecology and breeding tackling key problem research, have grasped the crucial Ecological Data of the aspects such as certain relevant its breeding, feeding habits, and have laid in more than 100 to reaching the breeding reserve parent fish of age at sexual maturity.Although many effort in early stage, still there are many 'bottleneck' restrictions in extensive artificial propagation and wild stocks amplification, and the artificial propagation difficulty of acipenser dabryanus is large and in the early stage exploratory stage.
Acipenser dabryanus artificial fecundation method is mainly artificial propagation techniques and the flow process with reference to similar sturgeon at present, too extensive, and acipenser dabryanus is that adaptation zone water ecological environment has formed its unique the propagational ecologic habits and characteristics during evolution, simply apply mechanically the artificial propagation techniques flow process of other sturgeons, effect is very undesirable, and main existing problems comprise: 1. male and female are asynchronous; 2. seminal fluid and ovum ratio are too extensive, can not adapt to large-scale breeding technology application; 3. reproduction rate, incubation rate are low; 4. fertilized egg percent of loss is high; 5. parent's lethality is higher.Therefore,, according to the physiological characteristic of acipenser dabryanus and reproductive characteristic, develop a set of characteristic acipenser dabryanus artificial propagation techniques flow process and be conducive to realize the extensive artificial propagation of whole process of acipenser dabryanus.
Summary of the invention
The object of the invention is physiology, ecosystem characterization for acipenser dabryanus uniqueness; develop a set of characteristic acipenser dabryanus artificial propagation techniques flow process; in solution acipenser dabryanus artificial propagation operating process, fineness is inadequate; fertilization rate, incubation rate are not high; realize the large-scale production of acipenser dabryanus fry, the artificial fecundation method of a kind of acipenser dabryanus is provided.
For achieving the above object, the technical solution used in the present invention is: the artificial fecundation method of a kind of acipenser dabryanus, the oxytocins that raun is injected at twice, double injection is spaced apart 12h, injection is for the first time 1/3～1/2 of accumulated dose, milter adopts a pin injection, and the time synchronizes with raun the first pin, half that dosage is raun; Adopt half-dried fertilization, water, ovum, seminal fluid ratio are mixed according to 75:50:1, stir 2～3min.
Further, described oxytocins is 1.5/kg of LHR-A26ug+ carp brain hypophysis.
Useful technique effect of the present invention is:
(1) the invention provides a set of complete acipenser dabryanus artificial propagation techniques flow process, for extensive artificial propagation provides technical support;
(2) adopt the inventive method, can effectively distinguish the maturity of acipenser dabryanus parent population, and improve acipenser dabryanus parent population spawning rate; By suitable compatibility of drugs, the ratio of seminal fluid, ovum, water, reaches as high as more than 85% acipenser dabryanus incubation rate; By adopting the current in the de-glutinous technology of fertilized egg, hatching process to control and triploidy rate, solved the restrictive factor in hatching process.
Embodiment
Below in conjunction with the embodiment of the present invention, the technical scheme in the embodiment of the present invention is clearly and completely described, obviously, described embodiment is only the present invention's part embodiment, rather than whole embodiment.Embodiment based in the present invention, those of ordinary skills, not making the every other embodiment obtaining under creative work prerequisite, belong to the scope of protection of the invention.
Embodiment 1
1, under natural conditions, water temperature starts to note the active situation of acipenser dabryanus in pond while reaching 16 ℃, and by the mode of belly perforation, gets ovum and observe, and the institute's ovum of getting heating is observed from middle incision of animal pole after boiling, and utilizes polarization index to judge acipenser dabryanus breeding sequential.
2, before artificial propagation, in miniflow pond, support 7 days temporarily, the ocyodinic of 1.5/kg of raun LH injection R-A26ug+ carp brain hypophysis, adopts biphasic injection, interval 12h, and the first pin is accumulated dose 1/3; Milter adopts a pin injection, and the time synchronizes with raun the first pin, half that dosage is raun; After pin, raun, milter are all supported temporarily in the pool, miniflow pond.(LHR-A2 is that Ningbo Sansheng Pharmaceutical Co., Ltd. produces Luteinizing hormone releasing-hormone A2).
3, after the first pin injection 20h, start to check in the pool, miniflow pond that acipenser dabryanus is from ovulation situation; According to maturity, respectively acipenser dabryanus is carried out to artificial propagation.
4, semen collection, inserts gonopore with conduit, seminal fluid access sealed bag, 4 ℃ of preservations after oxygenation.
5, with self-control scalpel, cut gonoduct, push gently belly and get ovum, adopt semi-dry process fertilization, water, ovum, seminal fluid ratio are mixed according to 75:50:1, gentle agitation 2min.
6, fertilized egg is cleaned 1 time with clear water, with food grade talcum powder suspension, mixes, and talcum powder suspension ratio is 10%, de-glutinous 30min after stirring 1min.
7, after de-sticking, with clear water, clean 2 times, proceed to sturgeon bottle formula incubator and hatch, in each hatching bottle, put 50,000 fertilized eggs, the water yield is controlled at 2m
3/ h, 18 ℃ of water temperatures.
8, until incubating oosperm, when little yolk plug forms the phase, measure fertilization rate, in every 12h suction hatching bottle, mould ovum once.
9, treat that incubating oosperm to hatching eye capsule forms after date, in every 4h suction hatching bottle, mould ovum once.
10, treat that incubating oosperm to tail reaches head after date, adopt Lotic hatching.
11, after fry cluster, after 1 day, proceed to cultivation cylinder and cultivate.
Embodiment 2
1, under natural conditions, water temperature starts to note the active situation of acipenser dabryanus in pond while reaching 16 ℃, and by the mode of belly perforation, gets ovum and observe, and the institute's ovum of getting heating is observed from middle incision of animal pole after boiling, and utilizes polarization index to judge acipenser dabryanus breeding sequential.
2, before artificial propagation, in miniflow pond, support 9 days temporarily, the ocyodinic of 1.5/kg of raun LH injection R-A26ug+ carp brain hypophysis, adopts biphasic injection, interval 12h, and the first pin is accumulated dose 1/2; Milter adopts a pin injection, and the time synchronizes with raun the first pin, half that dosage is raun; After pin, raun, milter are all supported temporarily in the pool, miniflow pond.(LHR-A2 is that Ningbo Sansheng Pharmaceutical Co., Ltd. produces Luteinizing hormone releasing-hormone A2).
3, after the first pin injection 20h, start to check in the pool, miniflow pond that acipenser dabryanus is from ovulation situation; According to maturity, respectively acipenser dabryanus is carried out to artificial propagation.
4, semen collection, inserts gonopore with conduit, seminal fluid access sealed bag, 4 ℃ of preservations after oxygenation.
5, with self-control scalpel, cut gonoduct, push gently belly and get ovum, adopt semi-dry process fertilization, water, ovum, seminal fluid ratio are mixed according to 75:50:1, gentle agitation 3min.
6, fertilized egg is cleaned 1 time with clear water, with food grade talcum powder suspension, mixes, and talcum powder suspension ratio is 10%, de-glutinous 30min after stirring 1min.
7, after de-sticking, with clear water, clean 2 times, proceed to sturgeon bottle formula incubator and hatch, in each hatching bottle, put 50,000 fertilized eggs, the water yield is controlled at 3m
3/ h, 18 ℃ of water temperatures.
8, until incubating oosperm, when little yolk plug forms the phase, measure fertilization rate, in every 12h suction hatching bottle, mould ovum once.
9, treat that incubating oosperm to hatching eye capsule forms after date, in every 4h suction hatching bottle, mould ovum once.
10, treat that incubating oosperm to tail reaches head after date, adopt Lotic hatching.
11, after fry cluster, after 1 day, proceed to cultivation cylinder and cultivate.
The foregoing is only preferred embodiment of the present invention, in order to limit the present invention, within the spirit and principles in the present invention not all, any modification of doing, be equal to replacement, improvement etc., within all should being included in protection scope of the present invention.
Claims (2)
1. an artificial fecundation method for acipenser dabryanus, is characterized in that, the oxytocins that raun is injected at twice, double injection is spaced apart 12h, and injection is for the first time 1/3～1/2 of accumulated dose, and milter adopts a pin injection, time synchronizes with raun the first pin, half that dosage is raun; Adopt half-dried fertilization, water, ovum, seminal fluid ratio are mixed according to 75:50:1, stir 2～3min.
2. the artificial fecundation method of acipenser dabryanus according to claim 1, is characterized in that, described oxytocins is 1.5/kg of LHR-A26ug+ carp brain hypophysis.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106069900A (en) * | 2016-06-15 | 2016-11-09 | 北京渔夫水产技术开发中心 | The method of a kind of Europe huso sturgeon artificial propagation and Cyprinus carpio application pituitary |
| CN106069899A (en) * | 2016-06-15 | 2016-11-09 | 中国水产科学研究院长江水产研究所 | Artificial regulatory realizes the method that acipenser dabryanus natural mating is laid eggs |
| CN110200725A (en) * | 2019-06-28 | 2019-09-06 | 中国长江三峡集团有限公司中华鲟研究所 | A kind of mandarin sturgeon hurtless measure semen collection method and device |
| CN112075365A (en) * | 2020-09-25 | 2020-12-15 | 四川省农业科学院水产研究所(四川省水产研究所) | Method for improving hatching rate of fertilized eggs of Acipenser changjiang |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106069900A (en) * | 2016-06-15 | 2016-11-09 | 北京渔夫水产技术开发中心 | The method of a kind of Europe huso sturgeon artificial propagation and Cyprinus carpio application pituitary |
| CN106069899A (en) * | 2016-06-15 | 2016-11-09 | 中国水产科学研究院长江水产研究所 | Artificial regulatory realizes the method that acipenser dabryanus natural mating is laid eggs |
| CN106069900B (en) * | 2016-06-15 | 2017-10-24 | 北京渔夫水产技术开发中心 | A kind of method of Europe huso sturgeon artificial propagation and carp application pituitary |
| CN110200725A (en) * | 2019-06-28 | 2019-09-06 | 中国长江三峡集团有限公司中华鲟研究所 | A kind of mandarin sturgeon hurtless measure semen collection method and device |
| CN112075365A (en) * | 2020-09-25 | 2020-12-15 | 四川省农业科学院水产研究所(四川省水产研究所) | Method for improving hatching rate of fertilized eggs of Acipenser changjiang |
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