CN103461251A - Method for artificially propagating triplophysa siluroides - Google Patents
Method for artificially propagating triplophysa siluroides Download PDFInfo
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- CN103461251A CN103461251A CN2013104498312A CN201310449831A CN103461251A CN 103461251 A CN103461251 A CN 103461251A CN 2013104498312 A CN2013104498312 A CN 2013104498312A CN 201310449831 A CN201310449831 A CN 201310449831A CN 103461251 A CN103461251 A CN 103461251A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention discloses a method for artificially propagating triplophysa siluroides. The method comprises the following steps of (1) selecting parent fish and hastening parturition: a, the selection of the parent fish, wherein 0.5-1.5 kg of healthy and strong mature parent fish without diseases and wounds are selected; b, hastening parturition by two needle injections, wherein the female fish with good maturity can be hastened for parturition when water temperature is 7-12 DEG C; the male fish are injected once, and the dosage is 2/5-3/5 of the total dosage injected into the female fish; c, semidry artificial insemination; (2) hatching fries by using a net cage or slow flowing water until yolk sacs of the fries are absorbed completely, wherein after the yolk sacs disappear, filtered egg yolk water are fed to the fries until the fries are able to swim in the water. By the method disclosed by the invention, the rate of fertilization, the rate of hatchability and rate of survival are high.
Description
technical field:
The present invention is relevant like the method for catfish Triplophysa with artificial propagation.
background technology:
Like the catfish Triplophysa (
triplophysa siluroides) the genus Cypriniformes, Cobitidae, Nemacheilinae, Genus Triplophysa.Be commonly called as: native catfish, pike.Be the plateau river fishes that one way of life is higher in height above sea level like the catfish Triplophysa, mainly be distributed in the upper reaches of the Yellow River and attached water body thereof.The hide section of in depth of waters such as, great tributaries, master stream rapid gravel substrate of normal happiness, also inhabit unhurried current and the hydrostatic water body of alluviation mud, many pasture and water, battalion's life of dwelling at the end.For predacious fish, adult fish be take the predation fish as main, and the juvenile fish drinking water is given birth to insect larvae.For kind maximum in the Cobitidae fish, the long 150-480mm of common individual body left and right, the long 482mm of maximum individual body, heavy 1.5kg.Because the plateau fish growth is very slow, and in the upper reaches of the Yellow River, one band high-quality fish are very few, not only individual large like the catfish Triplophysa, and muscle is plump, meat flavour is good, therefore be regarded as economic edible fishes, fish in a large number, result causes resource significantly to descend, and is put into " Chinese animals on the brink of extinction Red Data Book-fish " and " Chinese species Red List ", and grade in imminent danger is easily endanger (V).In order to save this resource, be not exterminated, taking artificial propagation is a kind of effective way, but, up to now still without any the message to like catfish Triplophysa successful reproduction, also without any document, shows to have the people once to carry out similarly work.
summary of the invention:
The objective of the invention is that a kind of fertilization rate is high in order to provide, incubation rate and the high artificial propagation of survival rate be like the method for catfish Triplophysa.
The object of the present invention is achieved like this:
The artificial breeding of the present invention is like the method for catfish Triplophysa, and the method comprises the following steps:
(1) selection of parent population with hasten parturition:
A. the selection of parent population: physique is healthy and strong, anosis without wound, the sexually matured parent population of 0.5～1.5kg:
B. two pin injections are hastened parturition: the good raun for maturity, and water temperature can be hastened parturition in the time of 7～12 ℃, and oxytocic drug and consumption are: LRH-A
2(luteotropin releasing hormone d-ala analog) every kg body weight is 3～6ug, the HCG(human chorionic gonadtropin) every kg body weight is 1000～1500IU, minute biphasic injection, injection is for the first time injected remaining all dosage for the 1/8～1/6, the second pin of accumulated dose; Milter adopts disposable injection, and dosage is 2/5～3/5 of raun accumulated dose;
C. semi-dry process artificial insemination: after drying the body surface water on ripe raun, hold the Nacl dilution in basin, then squeeze the ovum engagement, clamp-on seminal fluid simultaneously, stir with feather, sperm is fully contacted with ovum, now add again the clear water identical with Spawning water, activate sperm and ovum, after continuing to stir 1min, add the clean unnecessary seminal fluid of clear water rinsing and foreign material, pour the incubator box hatching into;
(2) hatching of employing net cage or miniflow water carry out fry hatching, until the absorption of prelarva yolk sac is complete, the yolk water that yolk sac should be thrown something and fed after disappearing after filtering is until put down trip, whole hatching process end.
Above-mentioned employing net cage hatching, net cage is placed the water surface, and Spawning water is Natural Water, dissolved oxygen >=5mg/L, 7～15 ℃ of hatching water temperatures, the pH value is 7.2～8.0, places 1 layer of ovum in case, adopt the hatching of miniflow water, should select in time bad ovum in hatching, the ovum of not inseminating, dead ovum, incubator box will be avoided illumination.
When above-mentioned semi-dry process workman inseminates, for seminal fluid, 5～10mL dilution rinses engagement, because of the milter seminal fluid less.
A large number of experiments show that: the artificial propagation method fertilization rate of the present invention can reach more than 70%, and incubation rate and survival rate can reach more than 80%, efficient recovery the upper reaches of the Yellow River can be arranged like catfish Triplophysa resource.
embodiment:
The artificial propagation of the present embodiment comprises the following steps like the method for catfish Triplophysa:
(1) selection of parent population with hasten parturition:
A. the selection of parent population
Select 0.5～1.5kg physique healthy and strong, anosis without wound, sexually matured parent population: the belly of raun expands softness, and the ovary profile is obvious, and gonopore swelling is slightly micro-red; Milter presses belly to have seminal fluid to flow out with have gentle hands, after water breakthrough, scatters immediately, is creamy white;
B. take two pin injections to hasten parturition: the good raun for maturity, water temperature can be hastened parturition in the time of 7～12 ℃, and oxytocic drug and consumption are: LRH-A
2every kg body weight is 4ug, and the every kg body weight of HCG is the 1250IU hybrid injection.Divide biphasic injection, injection for the first time, for for 1/8～1/6 of accumulated dose, is injected remaining all dosage for the second time.Milter all adopts disposable injection, and dosage is 1/2 of raun accumulated dose;
C. adopt semi-dry process artificial insemination: after ripe raun is dried to body surface water with dry towel, in basin, hold 0.9 the Nacl dilution of 100ml, then squeeze the ovum engagement, clamp-on seminal fluid simultaneously, the milter seminal fluid is less, therefore seminal fluid need rinse engagement with 5～10mL dilution, stir 2.5min with feather simultaneously, sperm is fully contacted with ovum, now add again the clear water 500mL identical with Spawning water, activate sperm and ovum, after continuing to stir 1min, add the clean unnecessary seminal fluid of 500mL clear water rinsing and foreign material, pour the incubator box hatching into, while carrying out artificial insemination, should note avoiding sunlight direct irradiation sperm and ovum,
(2) fry hatching:
A. adopt the net cage hatching, net cage is placed the water surface, and Spawning water is Natural Water, dissolved oxygen >=5mg/L, and 7-15 ℃ of hatching water temperatures, the pH value is 7.5, places 1 layer of ovum in case;
B. adopt miniflow water hatching: should select in time bad ovum in hatching, the ovum of not inseminating, dead ovum, incubator box will be avoided illumination;
C. maintain aforementioned hatching process until the absorption of prelarva yolk sac is complete, the yolk water that yolk sac should be thrown something and fed after disappearing after filtering is until put down trip, whole hatching process end.
Above-described embodiment is that foregoing of the present invention is further described, but this should be interpreted as to the scope of the above-mentioned theme of the present invention only limits to above-described embodiment.All technology realized based on foregoing all belong to scope of the present invention.
Claims (3)
1. artificial propagation is like the method for catfish Triplophysa, and the method comprises the following steps:
(1) selection of parent population with hasten parturition:
A. the selection of parent population: select physique healthy and strong, anosis without wound, the sexually matured parent population of 0.5～1.5kg:
B. two pin injections are hastened parturition:
The good raun for maturity, water temperature can be hastened parturition in the time of 7～12 ℃, and oxytocic drug and consumption are: LRH-A
2every kg body weight is 3～6ug, and the every kg body weight of HCG is 1000～1500IU, minute biphasic injection, and injection is for the first time injected remaining all dosage for the 1/8～1/6, the second pin of accumulated dose, and milter adopts disposable injection, and dosage is 2/5～3/5 of raun accumulated dose;
C. semi-dry process artificial insemination: after drying the body surface water on ripe raun, hold the Nacl dilution in basin, then squeeze the ovum engagement, clamp-on seminal fluid simultaneously, stir with feather, sperm is fully contacted with ovum, add again the clear water identical with Spawning water, activate sperm and ovum, after continuing to stir 1min, add the clean unnecessary seminal fluid of clear water rinsing and foreign material, pour the incubator box hatching into;
(2) hatching of employing net cage or miniflow water carry out fry hatching, until the absorption of prelarva yolk sac is complete, the yolk water that yolk sac is thrown something and fed after disappearing after filtering is until put down trip, whole hatching process end.
2. artificial propagation as claimed in claim 1 is like the method for catfish Triplophysa, it is characterized in that adopting the net cage hatching, net cage is placed the water surface, and Spawning water is Natural Water, dissolved oxygen >=5mg/L, 7～15 ℃ of hatching water temperatures, the pH value is 7.2～8.0, places 1 layer of ovum in case, adopts the hatching of miniflow water, should select in time bad ovum in hatching, the ovum of not inseminating, dead ovum, incubator box will be avoided illumination.
3. artificial propagation as claimed in claim 1 or 2 is like the method for catfish Triplophysa, 5～10mL dilution flushing engagement while it is characterized in that semi-dry process artificial insemination, for seminal fluid.
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| CN201310449831.2A CN103461251B (en) | 2013-09-29 | 2013-09-29 | Artificial propagation is like the method for catfish Triplophysa |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104335937A (en) * | 2014-08-08 | 2015-02-11 | 襄阳远丰农业科技发展有限公司 | Breeding method of Taiwan loaches |
| CN105052782A (en) * | 2015-07-15 | 2015-11-18 | 中国水产科学研究院长江水产研究所 | Artificial breeding method for Triplophysa xichangensis |
| CN105360028A (en) * | 2015-11-16 | 2016-03-02 | 华中农业大学 | Artificial breeding technology for Oriental loaches |
| CN106106293A (en) * | 2016-07-25 | 2016-11-16 | 云南华电鲁地拉水电有限公司 | A kind of artificial fecundation method of wild beautiful Triplophysa |
| CN107432261A (en) * | 2017-09-14 | 2017-12-05 | 四川省农业科学院水产研究所 | A kind of wild acclimation method like catfish Triplophysa |
| CN110876343A (en) * | 2019-06-20 | 2020-03-13 | 四川省农业科学院水产研究所(四川省水产研究所) | Method for improving artificial breeding efficiency of Leptobotia elongata |
| CN114868676A (en) * | 2022-03-08 | 2022-08-09 | 西双版纳云博水产养殖开发有限公司 | Spawning induction method suitable for male meinan silurus meridionalis |
| CN115777602A (en) * | 2022-11-11 | 2023-03-14 | 四川大学 | Ecological pond and domestication method for domesticating loaches with Holly horses |
Citations (2)
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| WO1999056534A1 (en) * | 1998-04-30 | 1999-11-11 | Ribes Andre | Method for reproducing and rearing silurus glanis in particular in aquatic medium and implementing device |
| RU2390992C1 (en) * | 2007-12-18 | 2010-06-10 | Государственное научное учреждение "Институт генетики и цитологии Национальной академии наук Беларуси" | METHOD OF ARTIFICIAL REPRODUCTION OF CATFISH (Silurus glanis L.) |
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- 2013-09-29 CN CN201310449831.2A patent/CN103461251B/en not_active Expired - Fee Related
Patent Citations (2)
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| WO1999056534A1 (en) * | 1998-04-30 | 1999-11-11 | Ribes Andre | Method for reproducing and rearing silurus glanis in particular in aquatic medium and implementing device |
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Non-Patent Citations (2)
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104335937A (en) * | 2014-08-08 | 2015-02-11 | 襄阳远丰农业科技发展有限公司 | Breeding method of Taiwan loaches |
| CN105052782A (en) * | 2015-07-15 | 2015-11-18 | 中国水产科学研究院长江水产研究所 | Artificial breeding method for Triplophysa xichangensis |
| CN105360028A (en) * | 2015-11-16 | 2016-03-02 | 华中农业大学 | Artificial breeding technology for Oriental loaches |
| CN106106293A (en) * | 2016-07-25 | 2016-11-16 | 云南华电鲁地拉水电有限公司 | A kind of artificial fecundation method of wild beautiful Triplophysa |
| CN107432261A (en) * | 2017-09-14 | 2017-12-05 | 四川省农业科学院水产研究所 | A kind of wild acclimation method like catfish Triplophysa |
| CN107432261B (en) * | 2017-09-14 | 2020-07-14 | 四川省农业科学院水产研究所 | Domestication method of wild silurus siluroides |
| CN110876343A (en) * | 2019-06-20 | 2020-03-13 | 四川省农业科学院水产研究所(四川省水产研究所) | Method for improving artificial breeding efficiency of Leptobotia elongata |
| CN110876343B (en) * | 2019-06-20 | 2022-02-08 | 四川省农业科学院水产研究所(四川省水产研究所) | Method for improving artificial breeding efficiency of Leptobotia elongata |
| CN114868676A (en) * | 2022-03-08 | 2022-08-09 | 西双版纳云博水产养殖开发有限公司 | Spawning induction method suitable for male meinan silurus meridionalis |
| CN114868676B (en) * | 2022-03-08 | 2022-11-11 | 西双版纳云博水产养殖开发有限公司 | Spawning induction method suitable for male meinan silurus meridionalis |
| CN115777602A (en) * | 2022-11-11 | 2023-03-14 | 四川大学 | Ecological pond and domestication method for domesticating loaches with Holly horses |
| CN115777602B (en) * | 2022-11-11 | 2024-04-23 | 四川大学 | Ecological pool for domesticating loaches and domestication method |
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| CN103461251B (en) | 2015-10-14 |
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