CN103461251B - Artificial propagation is like the method for catfish Triplophysa - Google Patents
Artificial propagation is like the method for catfish Triplophysa Download PDFInfo
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- CN103461251B CN103461251B CN201310449831.2A CN201310449831A CN103461251B CN 103461251 B CN103461251 B CN 103461251B CN 201310449831 A CN201310449831 A CN 201310449831A CN 103461251 B CN103461251 B CN 103461251B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
Artificial propagation of the present invention, like the method for catfish Triplophysa, comprising: the selection of (1) parent population with hasten parturition: the selection of a. parent population: selects that healthy and strong, the anosis nothing of physique is hindered, the sexually matured parent population of 0.5 ~ 1.5kg: b. bis-pin injection is hastened parturition; For the raun that maturity is good, water temperature can be hastened parturition 7 ~ 12 DEG C time, and milter adopts disposable injection, and dosage is 2/5 ~ 3/5 of raun accumulated dose; C. semi-dry process artificial insemination: (2) adopt net cage hatching or miniflow water to carry out fry hatching, until prelarva yolk sac absorbs complete, yolk sac should throw something and feed the yolk water after filtering till flat trip after disappearing.Fertilization rate of the present invention is high, incubation rate and survival rate high.
Description
technical field:
The present invention is relevant like the method for catfish Triplophysa with artificial propagation.
background technology:
Like catfish Triplophysa (
triplophysa siluroides) belong to Cypriniformes, Cobitidae, Nemacheilinae, Genus Triplophysa.Be commonly called as: native catfish, pike.Be one way of life in the higher plateau river fishes of height above sea level like catfish Triplophysa, be mainly distributed in the upper reaches of the Yellow River and attached water body thereof.The section of the gravel substrate that the depth of waters such as normal happiness is hidden in master stream, large tributary are rapid, also inhabits alluviation mud, the unhurried current of many pasture and water and hydrostatic water body, dwells life in the battalion end.For predacious fish, adult fish is to prey on fish, and juvenile fish drinking water gives birth to insect larvae.For kind maximum in Cobitidae fish, common individual body long about 150-480mm, the long 482mm of maximum individual body, heavy 1.5kg.Because plateau fish growth is very slow, and be with high-quality fish very few in the upper reaches of the Yellow River one, not only individual large like catfish Triplophysa, and muscle is plump, meat flavour is good, therefore be regarded as economic edible fishes and fish in a large number, result causes resource significantly to decline, and is put into " Chinese animals on the brink of extinction Red Data Book-fish " and " Chinese species Red List ", and threatened level is easily endanger (V).Not being exterminated to save this resource, taking artificial propagation to be a kind of effective way, but up to now still without any to the message like catfish Triplophysa successful reproduction, also showing have people once to carry out similar work without any document.
summary of the invention:
The object of the invention is to provide that a kind of fertilization rate is high, incubation rate and the high artificial propagation of survival rate be like the method for catfish Triplophysa.
The object of the present invention is achieved like this:
Artificial propagation of the present invention is like the method for catfish Triplophysa, and the method comprises the following steps:
(1) parent population selection with hasten parturition:
A. the selection of parent population: physique is healthy and strong, anosis without wound, the sexually matured parent population of 0.5 ~ 1.5kg:
B. two pin injections are hastened parturition: for the raun that maturity is good, and water temperature can be hastened parturition 7 ~ 12 DEG C time, and oxytocic drug and consumption are: LRH-A
2(luteotropin releasing hormone d-ala analog) every kg body weight is 3 ~ 6ug, HCG(human chorionic gonadtropin) every kg body weight is 1000 ~ 1500IU, point biphasic injection, all dosage that the 1/8 ~ 1/6, the second pin injection that first time injection is accumulated dose is remaining; Milter adopts disposable injection, and dosage is 2/5 ~ 3/5 of raun accumulated dose;
C. semi-dry process artificial insemination: after drying the body surface water on ripe raun, Nacl dilution is held in basin, then squeeze ovum engagement, clamp-on seminal fluid simultaneously, stir with feather, sperm is fully contacted with ovum, now add the clear water identical with Spawning water again, activate sperm and ovum, after continuing to stir 1min, add the unnecessary seminal fluid of clear water rinsed clean and foreign material, pour incubator box hatching into;
(2) adopt net cage hatching or miniflow water to carry out fry hatching, until prelarva yolk sac absorbs complete, yolk sac should throw something and feed the yolk water after filtering till flat trip after disappearing, whole hatching process terminates.
Above-mentioned employing net cage hatching, the water surface placed by net cage, and Spawning water is Natural Water, dissolved oxygen >=5mg/L, hatching water temperature 7 ~ 15 DEG C, pH value is 7.2 ~ 8.0, places 1 layer of ovum in case, adopt micro-Lotic hatching, in hatching, bad ovum should be selected in time, ovum of not inseminating, dead ovum, incubator box will avoid illumination.
When above-mentioned semi-dry process workman inseminates, seminal fluid 5 ~ 10mL dilution rinse engagement, because milter seminal fluid is less.
A large number of experiments show that: artificial fecundation method fertilization rate of the present invention can reach more than 70%, incubation rate and survival rate can reach more than 80%, can have efficient recovery the upper reaches of the Yellow River like catfish Triplophysa resource.
embodiment:
The artificial propagation of the present embodiment comprises the following steps like the method for catfish Triplophysa:
(1) parent population selection with hasten parturition:
A. the selection of parent population
Select 0.5 ~ 1.5kg physique healthy and strong, anosis without wound, sexually matured parent population: the belly of raun expands softness, and ovary profile is obvious, and gonopore swelling is slightly micro-red; Milter have gentle hands pressure belly has seminal fluid to flow out, and scatters immediately, be creamy white after water breakthrough;
B. take two pin injections to hasten parturition: for the raun that maturity is good, water temperature can be hastened parturition 7 ~ 12 DEG C time, and oxytocic drug and consumption are: LRH-A
2every kg body weight is the every kg body weight of 4ug, HCG is 1250IU hybrid injection.Divide biphasic injection, first time injection is for being 1/8 ~ 1/6 of accumulated dose, all dosage that injection is remaining for the second time.Milter all adopts disposable injection, and dosage is 1/2 of raun accumulated dose;
C. semi-dry process artificial insemination is adopted: after the raun of maturation is dried body surface water with dry towel, in basin, then hold the Nacl dilution of 0.9 of 100ml, then ovum engagement is squeezed, clamp-on seminal fluid simultaneously, milter seminal fluid is less, therefore seminal fluid need rinse engagement with 5 ~ 10mL dilution, stir 2.5min with feather simultaneously, sperm is fully contacted with ovum, now add the clear water 500mL identical with Spawning water again, activate sperm and ovum, after continuing to stir 1min, add the unnecessary seminal fluid of 500mL clear water rinsed clean and foreign material, pour incubator box hatching into, when carrying out artificial insemination, should note avoiding sunlight direct irradiation sperm and ovum,
(2) fry hatching:
A. adopt net cage hatching, the water surface placed by net cage, and Spawning water is Natural Water, dissolved oxygen >=5mg/L, hatching water temperature 7-15 DEG C, and pH value is 7.5, places 1 layer of ovum in case;
B. adopt micro-Lotic hatching: should select bad ovum in time in hatching, ovum of not inseminating, dead ovum, incubator box will avoid illumination;
C. maintain aforementioned hatching process until prelarva yolk sac absorbs complete, yolk sac should throw something and feed the yolk water after filtering till flat trip after disappearing, whole hatching process terminates.
Above-described embodiment is further described foregoing of the present invention, but this should be interpreted as that the scope of the above-mentioned theme of the present invention is only limitted to above-described embodiment.All technology realized based on foregoing all belong to scope of the present invention.
Claims (3)
1. artificial propagation is like the method for catfish Triplophysa, and the method comprises the following steps:
(1) parent population selection with hasten parturition:
A. the selection of parent population: select physique healthy and strong, anosis without wound, the sexually matured parent population of 0.5 ~ 1.5kg;
B. two pin injections are hastened parturition:
For the raun that maturity is good, water temperature can be hastened parturition 7 ~ 12 DEG C time, and oxytocic drug and consumption are: LRH-A
2every kg body weight is the every kg body weight of 3 ~ 6 μ g, HCG is 1000 ~ 1500IU, hybrid injection, divide biphasic injection, all dosage of the 1/8 ~ 1/6, the second pin injection remainder that first time injection is accumulated dose, milter adopts disposable injection, and dosage is 2/5 ~ 3/5 of raun accumulated dose;
C. semi-dry process artificial insemination: after drying the body surface water on ripe raun, NaCl dilution is held in basin, then squeeze ovum engagement, clamp-on seminal fluid simultaneously, stir with feather, sperm is fully contacted with ovum, add the clear water identical with Spawning water again, activate sperm and ovum, after continuing to stir 1min, add the unnecessary seminal fluid of clear water rinsed clean and foreign material, pour net cage hatching into;
(2) adopt net cage hatching and miniflow water carries out fry hatching, until prelarva yolk sac absorbs complete, yolk sac throws something and feeds the yolk water after filtering till flat trip after disappearing, whole hatching process terminates.
2. artificial propagation as claimed in claim 1 is like the method for catfish Triplophysa, it is characterized in that adopting net cage hatching, the water surface placed by net cage, and Spawning water is Natural Water, dissolved oxygen >=5mg/L, hatching water temperature 7 ~ 15 DEG C, pH value is 7.2 ~ 8.0, places 1 layer of ovum, adopt micro-Lotic hatching in case, should select bad ovum, unfertilized egg, dead ovum in hatching in time, net cage will avoid illumination.
3. artificial propagation as claimed in claim 1 or 2 is like the method for catfish Triplophysa, and when it is characterized in that semi-dry process artificial insemination, seminal fluid 5 ~ 10mL dilution rinses engagement.
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Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN104335937A (en) * | 2014-08-08 | 2015-02-11 | 襄阳远丰农业科技发展有限公司 | Breeding method of Taiwan loaches |
| CN105052782B (en) * | 2015-07-15 | 2016-03-23 | 中国水产科学研究院长江水产研究所 | The artificial fecundation method of Xichang Triplophysa |
| CN105360028B (en) * | 2015-11-16 | 2018-06-26 | 华中农业大学 | The artificial fecundation method of east Triplophysa |
| CN106106293A (en) * | 2016-07-25 | 2016-11-16 | 云南华电鲁地拉水电有限公司 | A kind of artificial fecundation method of wild beautiful Triplophysa |
| CN107432261B (en) * | 2017-09-14 | 2020-07-14 | 四川省农业科学院水产研究所 | Domestication method of wild silurus siluroides |
| CN110876343B (en) * | 2019-06-20 | 2022-02-08 | 四川省农业科学院水产研究所(四川省水产研究所) | Method for improving artificial breeding efficiency of Leptobotia elongata |
| CN114868676B (en) * | 2022-03-08 | 2022-11-11 | 西双版纳云博水产养殖开发有限公司 | Spawning induction method suitable for male meinan silurus meridionalis |
| CN115777602B (en) * | 2022-11-11 | 2024-04-23 | 四川大学 | Ecological pool for domesticating loaches and domestication method |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| RU2390992C1 (en) * | 2007-12-18 | 2010-06-10 | Государственное научное учреждение "Институт генетики и цитологии Национальной академии наук Беларуси" | METHOD OF ARTIFICIAL REPRODUCTION OF CATFISH (Silurus glanis L.) |
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| ES2254859T3 (en) * | 1998-04-30 | 2006-06-16 | Gilbert Ribes | PROCEDURE OF REPRODUCTION AND AGING IN A PARTICULARLY AQUATIC ENVIRONMENT OF SILURUS GLANIS AND DEVICE FOR STARTING UP. |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| RU2390992C1 (en) * | 2007-12-18 | 2010-06-10 | Государственное научное учреждение "Институт генетики и цитологии Национальной академии наук Беларуси" | METHOD OF ARTIFICIAL REPRODUCTION OF CATFISH (Silurus glanis L.) |
Non-Patent Citations (2)
| Title |
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| 似鲇高原鳅人工繁殖试验;李跃东等;《阿坝科技》;20091231;第45-46页 * |
| 塔里木河流域叶尔羌高原鳅人工繁殖试验;陈生熬等;《特产研究》;20081231(第4期);第28-30页 * |
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