CN107683798A - A kind of Pelteobagrus fulvidraco artificial breeding and the method for raising - Google Patents
A kind of Pelteobagrus fulvidraco artificial breeding and the method for raising Download PDFInfo
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- CN107683798A CN107683798A CN201710888306.9A CN201710888306A CN107683798A CN 107683798 A CN107683798 A CN 107683798A CN 201710888306 A CN201710888306 A CN 201710888306A CN 107683798 A CN107683798 A CN 107683798A
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- 241000376029 Tachysurus fulvidraco Species 0.000 title claims abstract description 114
- 238000000034 method Methods 0.000 title claims abstract description 26
- 238000009395 breeding Methods 0.000 title claims abstract description 14
- 230000001488 breeding effect Effects 0.000 title claims abstract description 11
- 229910052500 inorganic mineral Inorganic materials 0.000 claims abstract description 40
- 239000011707 mineral Substances 0.000 claims abstract description 40
- 239000012530 fluid Substances 0.000 claims abstract description 24
- 239000003755 preservative agent Substances 0.000 claims abstract description 24
- 230000002335 preservative effect Effects 0.000 claims abstract description 24
- 239000003643 water by type Substances 0.000 claims abstract description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 61
- 102000002322 Egg Proteins Human genes 0.000 claims description 30
- 108010000912 Egg Proteins Proteins 0.000 claims description 30
- 210000004681 ovum Anatomy 0.000 claims description 30
- 230000000384 rearing effect Effects 0.000 claims description 29
- 238000002347 injection Methods 0.000 claims description 27
- 239000007924 injection Substances 0.000 claims description 27
- 230000012447 hatching Effects 0.000 claims description 24
- 238000006213 oxygenation reaction Methods 0.000 claims description 20
- 210000000582 semen Anatomy 0.000 claims description 20
- 241000251468 Actinopterygii Species 0.000 claims description 15
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- 230000037396 body weight Effects 0.000 claims description 15
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- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 claims description 10
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 10
- 239000000203 mixture Substances 0.000 claims description 10
- 239000011148 porous material Substances 0.000 claims description 10
- 229910052711 selenium Inorganic materials 0.000 claims description 10
- 239000011669 selenium Substances 0.000 claims description 10
- 230000009027 insemination Effects 0.000 claims description 8
- 238000001665 trituration Methods 0.000 claims description 8
- 238000002156 mixing Methods 0.000 claims description 6
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 claims description 5
- 235000017060 Arachis glabrata Nutrition 0.000 claims description 5
- 241001553178 Arachis glabrata Species 0.000 claims description 5
- 235000010777 Arachis hypogaea Nutrition 0.000 claims description 5
- 235000018262 Arachis monticola Nutrition 0.000 claims description 5
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 5
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 claims description 5
- 241000243686 Eisenia fetida Species 0.000 claims description 5
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 5
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims description 5
- 241001597008 Nomeidae Species 0.000 claims description 5
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 claims description 5
- 239000005864 Sulphur Substances 0.000 claims description 5
- 239000002253 acid Substances 0.000 claims description 5
- 150000007513 acids Chemical class 0.000 claims description 5
- 238000013019 agitation Methods 0.000 claims description 5
- 125000001246 bromo group Chemical class Br* 0.000 claims description 5
- 239000011575 calcium Substances 0.000 claims description 5
- 229910052791 calcium Inorganic materials 0.000 claims description 5
- 238000004140 cleaning Methods 0.000 claims description 5
- 239000010949 copper Substances 0.000 claims description 5
- 229910052802 copper Inorganic materials 0.000 claims description 5
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 claims description 5
- 229910000397 disodium phosphate Inorganic materials 0.000 claims description 5
- JEGUKCSWCFPDGT-UHFFFAOYSA-N h2o hydrate Chemical compound O.O JEGUKCSWCFPDGT-UHFFFAOYSA-N 0.000 claims description 5
- PNDPGZBMCMUPRI-UHFFFAOYSA-N iodine Chemical compound II PNDPGZBMCMUPRI-UHFFFAOYSA-N 0.000 claims description 5
- 229910052742 iron Inorganic materials 0.000 claims description 5
- 238000002386 leaching Methods 0.000 claims description 5
- 239000011777 magnesium Substances 0.000 claims description 5
- 229910052749 magnesium Inorganic materials 0.000 claims description 5
- 230000035800 maturation Effects 0.000 claims description 5
- 229910052757 nitrogen Inorganic materials 0.000 claims description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N nitrogen Substances N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 5
- 235000020232 peanut Nutrition 0.000 claims description 5
- 239000011734 sodium Substances 0.000 claims description 5
- 229910052708 sodium Inorganic materials 0.000 claims description 5
- 239000011780 sodium chloride Substances 0.000 claims description 5
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 claims description 4
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims description 4
- 239000007788 liquid Substances 0.000 claims description 4
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 claims description 2
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 2
- 239000007836 KH2PO4 Substances 0.000 claims description 2
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 2
- 229910000147 aluminium phosphate Inorganic materials 0.000 claims description 2
- 239000001110 calcium chloride Substances 0.000 claims description 2
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 2
- 239000004202 carbamide Substances 0.000 claims description 2
- 229910001629 magnesium chloride Inorganic materials 0.000 claims description 2
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 2
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 2
- 239000000745 gonadal hormone Substances 0.000 claims 1
- 239000002434 gonadorelin derivative Substances 0.000 claims 1
- 230000004720 fertilization Effects 0.000 abstract description 13
- 244000045947 parasite Species 0.000 abstract description 8
- 230000004083 survival effect Effects 0.000 abstract description 5
- 238000011534 incubation Methods 0.000 abstract description 4
- 238000005457 optimization Methods 0.000 abstract description 4
- 239000007921 spray Substances 0.000 abstract description 2
- 102000011022 Chorionic Gonadotropin Human genes 0.000 description 11
- 108010062540 Chorionic Gonadotropin Proteins 0.000 description 11
- 229940084986 human chorionic gonadotropin Drugs 0.000 description 10
- 229940088597 hormone Drugs 0.000 description 8
- 239000005556 hormone Substances 0.000 description 8
- 239000005696 Diammonium phosphate Substances 0.000 description 6
- 208000030852 Parasitic disease Diseases 0.000 description 6
- 210000003298 dental enamel Anatomy 0.000 description 6
- MNNHAPBLZZVQHP-UHFFFAOYSA-N diammonium hydrogen phosphate Chemical compound [NH4+].[NH4+].OP([O-])([O-])=O MNNHAPBLZZVQHP-UHFFFAOYSA-N 0.000 description 6
- 229910000388 diammonium phosphate Inorganic materials 0.000 description 6
- 235000019838 diammonium phosphate Nutrition 0.000 description 6
- 241000239250 Copepoda Species 0.000 description 4
- 241001000394 Diaphania hyalinata Species 0.000 description 4
- -1 LRH-A2 Proteins 0.000 description 4
- 239000003488 releasing hormone Substances 0.000 description 4
- 241000256135 Chironomus thummi Species 0.000 description 3
- 235000019750 Crude protein Nutrition 0.000 description 3
- 241001500087 Trichodina Species 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 230000001186 cumulative effect Effects 0.000 description 3
- 239000004744 fabric Substances 0.000 description 3
- 210000003746 feather Anatomy 0.000 description 3
- 238000011049 filling Methods 0.000 description 3
- 238000012544 monitoring process Methods 0.000 description 3
- 239000004570 mortar (masonry) Substances 0.000 description 3
- 230000003071 parasitic effect Effects 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 230000001105 regulatory effect Effects 0.000 description 3
- 238000005070 sampling Methods 0.000 description 3
- WESJNFANGQJVKA-UHFFFAOYSA-M sodium;2,3-dichloro-2-methylpropanoate Chemical compound [Na+].ClCC(Cl)(C)C([O-])=O WESJNFANGQJVKA-UHFFFAOYSA-M 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- 210000002700 urine Anatomy 0.000 description 3
- 241001262815 Dactylogyrus Species 0.000 description 2
- 241001523601 Gyrodactylus Species 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 210000001061 forehead Anatomy 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 241000252233 Cyprinus carpio Species 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004246 corpus luteum Anatomy 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000002196 ecbolic effect Effects 0.000 description 1
- 210000004943 gill filament Anatomy 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 210000003097 mucus Anatomy 0.000 description 1
- 230000003287 optical effect Effects 0.000 description 1
- 230000032696 parturition Effects 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 238000009738 saturating Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000009303 sperm storage Effects 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- LEDMRZGFZIAGGB-UHFFFAOYSA-L strontium carbonate Chemical compound [Sr+2].[O-]C([O-])=O LEDMRZGFZIAGGB-UHFFFAOYSA-L 0.000 description 1
- 229910000018 strontium carbonate Inorganic materials 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
- Fodder In General (AREA)
Abstract
The invention discloses a kind of Pelteobagrus fulvidraco artificial breeding and the method for raising, the inventive method is by using special spermatozoa preservative fluid, specific 27 29 DEG C of warm mineral waters, the technological means such as optimization parent culture condition significantly improve Pelteobagrus fulvidraco spawning rate (up to 95.3%), rate of fertilization (up to 93.7%), incubation rate (up to 94.1%), spray emergence rate (up to 93.8%) and seed survival rate (up to 75.4%), and it can be bred in can realizing Pelteobagrus fulvidraco seedling at all seasons, Pelteobagrus fulvidraco seedling and the commodity Pelteobagrus fulvidraco that no parasite carries can be cultivated, the time more than 20% that Pelteobagrus fulvidraco seedling is cultivated and commodity Pelteobagrus fulvidraco is formed can be shortened, remarkable in economical benefits.
Description
Technical field
The invention belongs to propagate field artificially, and in particular to a kind of Pelteobagrus fulvidraco artificial breeding and the method for raising.
Background technology
Pelteobagrus fulvidraco is distributed widely in the rivers in the Changjiang river and Pearl River system, in lake, and You Yizhong, downstream lake are more.Cause
Its fine and tender taste is delicious and cultivates species as the new famous-particular-excellent in China, is one of important small-sized economic fish.It is big with four
Chinese carp is compared, and the spawning rate of Pelteobagrus fulvidraco, rate of fertilization, incubation rate and seed survival rate are relatively low so that Pelteobagrus fulvidraco seedling is difficult to meet city
The demand of field (particularly in winter-spring season).
Parasitic disease is one of Pelteobagrus fulvidraco Major Diseases, as the bacterial complications as caused by ich often give raiser
Bring heavy economic losses.Under many circumstances, Pelteobagrus fulvidraco parasitic disease is due to the Pelteobagrus fulvidraco seedling of raiser's purchase
Kind just carries parasitic agent.Therefore, cultivate the Pelteobagrus fulvidraco seedling that no parasite carries be preventing and treating Pelteobagrus fulvidraco parasitic disease and its
One important step of complication.
The content of the invention
It is an object of the invention to provide a kind of Pelteobagrus fulvidraco artificial breeding and the method for raising.
The technical solution used in the present invention is：
A kind of Pelteobagrus fulvidraco artificial breeding and the method for raising, comprise the following steps：
1) Pelteobagrus fulvidraco parent is raised with 27-29 DEG C of warm mineral water, raised 7~10 days, then to Pelteobagrus fulvidraco parent
This progress artificial induced spawning；
2) artificial insemination：After artificial induced spawning 16-20h, male spermaries are taken, grind to obtain spermary trituration, add sperm storage
Liquid, mix, obtain Pelteobagrus fulvidraco seminal fluid, while female adult ovum is taken out；By the leaching of Pelteobagrus fulvidraco seminal fluid on ovum, mix, wherein yellow forehead
The volume ratio of milt liquid and ovum is 0.2~0.4：1；And then a small amount of 27-29 DEG C of warm mineral water is added, mix, standing 3~
8min, embryonated egg is cleaned up；
The formula of the spermatozoa preservative fluid is：Every liter of spermatozoa preservative fluid contains 6.5~7.5g NaCl, 0.4~0.6g
MgCl2, 0.8~1.2g Na2HPO4·7H2O, 0.7~0.9g KH2PO4, 1.4~1.6g CaCl2, 0.01~0.02g
SrCO3, 0.1~0.2g KIO3, 0.5~1.0g 1B selenium, surplus is water, and pH is 7~7.5；
3) Pelteobagrus fulvidraco embryonated egg is uniformly sprinkling upon in 27-29 DEG C of warm mineral water hatchery for be laid with fish nest and hatched,
Oxygenation is carried out using micro-pore oxygenation mode；
4) initial stage raises after incubating oosperm：After incubating oosperm, it is 27-29 DEG C to maintain hatchery temperature mineral water water temperature,
Abundant oxygenation is carried out using micro-pore oxygenation mode；The 3rd~7 day after hatching, 2 wheel animalcules are fed daily, are needed every time by every tail fry
1-5 wheel animalcule standard is fed；
5) advanced fry of yellow catfish is cultivated：Before Pelteobagrus fulvidraco seedling is transferred to seed rearing pool, using 83 DEG C or so warm mineral waters by seedling
Kind of rearing pool temperature adjustment simultaneously maintains 27-29 DEG C；The advanced fry of yellow catfish of the 9th~11 day after hatching is transferred to seed rearing pool
Constant temperature raising is carried out, stocking density is 2.5 × 105~3.5 × 105Tail/mu；2 artemia naupiar larvas of feeding daily, raise every time
Amount is fed as the 4.5~5.5% of fry body weight；
6) feeding management：Carry out later stage feeding management within the 28th~33 day after hatching, water level is maintained 1-1.5m, water is saturating
Lightness is maintained at 20-30cm, the ammonium of application of phosphoric acid two and urea, is defined by water ammonia nitrogen no more than 1mg/L；Daily feeding 2 times it is red
Worm, each scale of feeding is the 4.5~5.5% of fry body weight, until fry reaches specification；
In above-mentioned temperature mineral water containing 225~275 μ g/L sodium, 0.5~0.7 μ g/L iron, 0.6~0.8 μ g/L iodine, 0.3~
0.5 μ g/L bromines, 0.05~0.07 μ g/L sulphur, 1.4~1.6 μ g/L metasilicic acids, 1.1~1.3 μ g/L heavy carbonics, 280~320 μ g/L
Calcium, 0.6~0.8 μ g/L magnesium, 0.001~0.003 μ g/L lithiums, 0.1~0.14 μ g/L selenium, 0.02~0.06 μ g/L copper, 0.02~
0.04 μ g/L strontiums.
Further, in step 1), it is female-male proportion 13 more than age at the age that Pelteobagrus fulvidraco parent, which is,:0.8~1.2.
Further, in step 1), the operation of artificial induced spawning is：Generated using by human chorionic gonadotropin HCG, rush corpus luteum
The mixing derivant of hormone-releasing hormone analog LRH-A and DOM DOM compositions induces yellow forehead in 27-29 DEG C of warm mineral water
The maturation of fish female and male gametophyte, per kg body weight female Pelteobagrus fulvidraco HCG, LRH-A2, DOM of injection be respectively 1100~1300IU,
24~26 μ g, 9~11 μ g；Using double injection method, the 1st injection dosage is total amount 2/9~2/7, is noted for the second time after 9~11h
Penetrate；Milter dosage halves, and only injects 1 time, is synchronously injected in the 2nd injection of raun.
Further, in step 2), it is 1g that spermary, which grinds liquid and the mass volume ratio of spermatozoa preservative fluid,:0.8~1.2ml.
Further, in step 3), the density that Pelteobagrus fulvidraco embryonated egg is sprinkling upon the hatchery for being laid with fish nest is 2.5~3.4
Ten thousand ovum/square metre.
Further, in step 5), seed rearing pool first passes through in advance cleans up the pond and cultivates food organisms cultivation.
Further, it is described to clean up the pond to kill wild fishes, the spiral shell in seed rearing pool.
Further, the concrete operations that the food organisms are cultivated are：The 4th~6 day after cleaning up the pond, in seed rearing pool
28~32 kgs/acre of peanut press pulps are added, new water, agitation mud is injected, cultivates food organisms.
Further, the food organisms are wheel animalcule, cladocera and/or Copepods biology.
The beneficial effects of the invention are as follows：
By the present invention in that pond temperature is adjusted with special Pelteobagrus fulvidraco spermatozoa preservative fluid, using 83 DEG C or so warm mineral waters
Save and maintain 27-29 DEG C, optimization oxytocic hormone proportioning, the optimization technological means such as parent culture condition hastened parturition with improving Pelteobagrus fulvidraco
Rate, rate of fertilization, incubation rate and seed survival rate, breeding more than two weeks time, can cultivating without small melonworm for Pelteobagrus fulvidraco can be shifted to an earlier date
Deng parasite carry advanced fry of yellow catfish, can shorten Pelteobagrus fulvidraco cultivate the time more than 20%, remarkable in economical benefits.
Embodiment
For the ease of the understanding of those skilled in the art, present invention work is further retouched with reference to specifically embodiment
State.
A kind of Pelteobagrus fulvidraco artificial breeding of embodiment 1 and the method for raising
Present invention is disclosed it is a kind of using warm mineral water, spermatozoa preservative fluid carry out advanced fry of yellow catfish artificial breeding method,
Comprise the following steps：
1) 27 DEG C are maintained pond temperature adjustment and using 83 DEG C or so of warm mineral water.Temperature in use sensor is to water
Temperature is monitored in real time.27 DEG C of temperature will be transferred in the common pond reinforced cultivating 3 Pelteobagrus fulvidraco parent more than age of more than 30 days
In mineral water, Pelteobagrus fulvidraco parent reinforced cultivating is carried out 10 days, and feed high protein feed (crude protein ratio is 39%), per in the sky
Noon 9：00, afternoon 16：00, once, feeding is to eat up as amount in 1 hour every time for each feeding.
2) the Pelteobagrus fulvidraco parent after reinforced cultivating is subjected to artificial induced spawning, using yellow by human chorionic gonadotropin (HCG), rush
The mixing derivant of body generation hormone-releasing hormone analog 2 (LRH-A2) and DOM (DOM) composition induces Pelteobagrus fulvidraco at 29 DEG C
The maturation of female and male gametophyte.HCG, LRH-A2, DOM per the injection of kg body weight female Pelteobagrus fulvidraco are respectively 1200IU, 25 micrograms, 10
Milligram；Using double injection method, the 1st injection dosage is that total amount is injected for second after 1/4,10 hours；Milter dosage halves, and
Only injection 1 time, synchronously injected in the 2nd injection of raun.
3) artificial insemination：
After second injection of hormone 16-20 hours, raun start heat, gametocide Pelteobagrus fulvidraco, spermary is taken, in mortar
Spermary trituration is ground to obtain, adds the ratio of 1ml spermatozoa preservative fluids to add spermatozoa preservative fluid according to 1 gram of spermary trituration, mixing is equal
It is even, obtain the Pelteobagrus fulvidraco seminal fluid for fertilization；
The formula of the spermatozoa preservative fluid is：Every liter of spermatozoa preservative fluid contains 6.5g NaCl, 0.6g MgCl2、1.2g
Na2HPO4·7H2O、0.7g KH2PO4、1.4g CaCl2、0.01g SrCO3、0.2g KIO3, 0.5g 1B selenium, surplus
For water, pH is 7~7.5；
After preparing seminal fluid, artificial insemination is carried out immediately.While mature egg is squeezed into dry white enamel jorum, use
Syringe (not having to syringe needle) is drawn the seminal fluid leaching prepared and is gently mixed on ovum, and with feather.But Pelteobagrus fulvidraco ovum occupies white enamel
During 4/5ths space of jorum, stopping is squeezed into Pelteobagrus fulvidraco ovum and drenched into seminal fluid, and the wherein volume ratio of seminal fluid and ovum is 0.3：1.It
The 27-29 DEG C of warm mineral water that volume is cumulative volume 5% is added afterwards, is mixed, and is stood 5min, ovum is fully combined with sperm.Will be complete
Ovum into fertilization procedures rinses several times, washes away watery blood.Spawning rate is counted, the results are shown in Table 1.
4) Pelteobagrus fulvidraco egg hatching.According to 30,000 ovum/square metre standard, Pelteobagrus fulvidraco embryonated egg is uniformly spread out into cloth and is being laid with
Hatched in 27-29 DEG C of warm mineral water hatchery of fish nest, and oxygenation is carried out using micro-pore oxygenation mode.1500 are taken to be subjected to
The Pelteobagrus fulvidraco ovum of precision processing, after by precision processing 30 minutes, fertilization situation is observed under 50 times of ordinary optical microscopes, count by
Smart rate, the results are shown in Table 1.
5) initial stage raises after incubating oosperm：Hatchery temperature mineral water water temperature is maintained to use micro-pore oxygenation side at 27-29 DEG C
Formula carries out abundant oxygenation, from hatching the 3rd day to the 7th day, every morning 9：00, afternoon 16：00, a wheel animalcule is respectively fed, by every
Tail fry needs 1-5 wheel animalcule standard to feed every time.When fry can put down trip, Pelteobagrus fulvidraco seedling emergence rate is counted, is shown in Table 1.
6) advanced fry of yellow catfish is cultivated, and is specifically included：(1) clean up the pond, wild fishes, spiral shell in killing seed rearing pool etc..(2) train
Educate food organisms.The 5th day after cleaning up the pond, 28~32 kgs/acre of peanut press pulps are added in seed rearing pool, inject new water, agitation
The food organisms such as mud, culture of rotifera, cladocera and Copepods.(3) before Pelteobagrus fulvidraco seedling is transferred to seed rearing pool, 83 DEG C are used
Left and right temperature mineral water is by seed rearing pool temperature adjustment and maintains 27-29 DEG C.(4) fry is put in a suitable place to breed.According to 300,000 tails/mu mark
Standard, the Pelteobagrus fulvidraco seedling of the 9th day after hatching is transferred to the seed rearing pool (water temperature maintains 27-29 DEG C) after sterilization, raised daily
2 artemia naupiar larvas are fed, each scale of feeding is the 5% of fry body weight.
7) feeding management：Proceed by within the 28th day after hatching later stage feeding management, regular water filling, water level maintain 1.0 meters-
Between 1.5 meters.Regulating water quality and fertile degree.Water transparence is maintained between 20-30 centimetres, and dose is (with Diammonium phosphate (DAP) and urine
Based on element) it is defined by water ammonia nitrogen no more than 1mg/L.Every morning 9：00, afternoon 16：00, each red worm of feeding (midge children
Worm), each scale of feeding is the 5% of fry body weight.When fry specification reaches 5-6cm, you can press summer flower sale, and analyze Pelteobagrus fulvidraco
Survival rate of fish fry (the results are shown in Table 1).
In above-mentioned temperature mineral water containing 275 μ g/L sodium, 0.7 μ g/L iron, 0.8 μ g/L iodine, 0.3 μ g/L bromines, 0.05 μ g/L sulphur,
1.6 μ g/L metasilicic acids, 1.3 μ g/L heavy carbonics, 280 μ g/L calcium, 0.8 μ g/L magnesium, 0.003 μ g/L lithiums, 0.1 μ g/L selenium, 0.06 μ g/
L copper, 0.02 μ g/L strontiums.
The Pelteobagrus fulvidraco artificial induced spawning of table 1, fertilization, hatching and seed rearing result
From table 1 it follows that the present invention is significantly improved by optimizing parent culture, fertilization, hatching and rearing conditions
The spawning rate (up to 95.3%) of Pelteobagrus fulvidraco, rate of fertilization (up to 93.7%), incubation rate (up to 94.1%), spray emergence rate (reach
And seed survival rate 93.8%) (up to 75.4%)；Significantly improve the growth rate of Pelteobagrus fulvidraco, after fertilization 24 days or so, body length
Up to more than 3cm, the nursery speed of growth fast more than 20% of more conventional method cultivation；And Pelteobagrus fulvidraco can be realized at all seasons
In can breed.
Pelteobagrus fulvidraco (experimental group), the commercially available Pelteobagrus fulvidraco (control group) obtained below to the present embodiment live box carries out parasite inspection
Survey and statistical analysis occurs for parasitic disease.
Parasite monitoring method
The Pelteobagrus fulvidraco gill filament and epidermal mucus that the present embodiment live box obtains is taken to carry out Pelteobagrus fulvidraco under 400 power microscopes common
The microscopy (pond of every 5 mu of sizes, test samples number are 20 tails) of parasitic agent, every 10 days sampling monitorings once (from
Start within the 20th day after hatching).Control group sample is derived from local and periphery fry field, per sub-sampling 3, every 50 tails of sampling.
As a result：
Assay is as shown in table 2, there it can be seen that 20 days always after hatching for the Pelteobagrus fulvidraco of the technology of the present invention raising
80 days after to hatching, Pelteobagrus fulvidraco is without parasitic infections such as small melonworm, Trichodina, Dactylogyrus, Gyrodactylus, and present conventional method
The parasitic disease such as small melonworm, Trichodina is multiple in Pelteobagrus fulvidraco under cultivating and raising.The above results illustrate that the present invention passes through optimization
Parent culture, fertilization, hatching and rearing conditions, the infection of parasitic pathogen can be effectively avoided, improve the safety of product Pelteobagrus fulvidraco
Property.
The Pelteobagrus fulvidraco parasite monitoring result of table 2
| Group | Small melonworm | Trichodina | Dactylogyrus | Gyrodactylus |
| Experimental group monitors parasite number | 0 | 0 | 0 | 0 |
| Control group monitors parasite number | 2 | 1 | 0 | 1 |
A kind of Pelteobagrus fulvidraco artificial breeding of embodiment 2 and the method for raising
Present invention is disclosed it is a kind of using warm mineral water, spermatozoa preservative fluid carry out advanced fry of yellow catfish artificial breeding method,
Comprise the following steps：
1) 29 DEG C are maintained pond temperature adjustment and using 83 DEG C or so of warm mineral water.Temperature in use sensor is to water
Temperature is monitored in real time.29 DEG C of temperature will be transferred in the common pond reinforced cultivating 3 Pelteobagrus fulvidraco parent more than age of more than 30 days
In mineral water, female-male proportion 1:0.8, carry out Pelteobagrus fulvidraco parent reinforced cultivating 7 days, and feed high protein feed (ratio of crude protein
Example is 39%) every morning 9：00, afternoon 16：00, once, feeding is to eat up as amount in 1 hour every time for each feeding.
2) the Pelteobagrus fulvidraco parent after reinforced cultivating is subjected to artificial induced spawning, using yellow by human chorionic gonadotropin (HCG), rush
The mixing derivant of body generation hormone-releasing hormone analog 2 (LRH-A2) and DOM (DOM) composition induces Pelteobagrus fulvidraco at 29 DEG C
The maturation of female and male gametophyte.HCG, LRH-A2, DOM per the injection of kg body weight female Pelteobagrus fulvidraco are respectively 1200IU, 25 micrograms, 10
Milligram；Using double injection method, the 1st injection dosage is that total amount is injected for second after 2/9,11 hours；Milter dosage halves, and
Only injection 1 time, synchronously injected in the 2nd injection of raun.
3) artificial insemination：
After second injection of hormone 20 hours, raun start heat, gametocide Pelteobagrus fulvidraco, spermary is taken, is ground in mortar
It is broken that spermary trituration, the ratio for adding 1.2ml spermatozoa preservative fluids according to 1 gram of spermary trituration add spermatozoa preservative fluid, mixed equal
It is even, obtain the Pelteobagrus fulvidraco seminal fluid for fertilization；
The formula of the spermatozoa preservative fluid is：Every liter of spermatozoa preservative fluid contains 7.5g NaCl, 0.4g MgCl2、0.8g
Na2HPO4·7H2O、0.9g KH2PO4、1.6g CaCl2、0.02g SrCO3、0.1g KIO3, 1.0g 1B selenium, surplus
For water, pH 7；
After preparing seminal fluid, artificial insemination is carried out immediately.While mature egg is squeezed into dry white enamel jorum, use
Syringe (not having to syringe needle) is drawn the seminal fluid leaching prepared and is gently mixed on ovum, and with feather.But Pelteobagrus fulvidraco ovum occupies white enamel
During 4/5ths space of jorum, stopping is squeezed into Pelteobagrus fulvidraco ovum and drenched into seminal fluid, and the wherein volume ratio of seminal fluid and ovum is 0.3：1.It
29 DEG C of warm mineral waters that volume is cumulative volume 5% are added afterwards, are mixed, and are stood 5min, ovum is fully combined with sperm.Will complete by
The ovum rinsing of essence operation several times, washes away watery blood.
4) Pelteobagrus fulvidraco egg hatching.According to 2.5 ten thousand ovum/square metre standard, Pelteobagrus fulvidraco embryonated egg is uniformly spread out into cloth and laid
Have in 29 DEG C of warm mineral water hatcheries of fish nest and hatched, and oxygenation is carried out using micro-pore oxygenation mode.
5) initial stage raises after incubating oosperm：Hatchery temperature mineral water water temperature is maintained to use micro-pore oxygenation mode at 29 DEG C
Abundant oxygenation is carried out, from hatching the 3rd day to the 7th day, every morning 9：00, afternoon 16：00, a wheel animalcule is respectively fed, by every tail
Fry needs 1-5 wheel animalcule standard to feed every time.
6) advanced fry of yellow catfish is cultivated, and is specifically included：(1) clean up the pond, wild fishes, spiral shell in killing seed rearing pool etc..(2) train
Educate food organisms.The 5th day after cleaning up the pond, 28~32 kgs/acre of peanut press pulps are added in seed rearing pool, inject new water, agitation
The food organisms such as mud, culture of rotifera, cladocera and Copepods.(3) before Pelteobagrus fulvidraco seedling is transferred to seed rearing pool, 83 DEG C are used
Left and right temperature mineral water is by seed rearing pool temperature adjustment and maintains 29 DEG C.(4) fry is put in a suitable place to breed., will according to 350,000 tails/mu standard
The Pelteobagrus fulvidraco seedling of the 9th day is transferred to the seed rearing pool (water temperature maintains 27-29 DEG C) after sterilization after hatching, daily feeding 2 times
Artemia naupiar larva, each scale of feeding are the 5% of fry body weight.
7) feeding management：Proceed by within the 33rd day after hatching later stage feeding management, regular water filling, water level maintain 1.0 meters-
Between 1.5 meters.Regulating water quality and fertile degree.Water transparence is maintained between 20-30 centimetres, and dose is (with Diammonium phosphate (DAP) and urine
Based on element) it is defined by water ammonia nitrogen no more than 1mg/L.Every morning 9：00, afternoon 16：00, each red worm of feeding (midge children
Worm), each scale of feeding is the 5% of fry body weight.When fry specification reaches 5-6cm, you can press summer flower sale.
In above-mentioned temperature mineral water containing 225 μ g/L sodium, 0.5 μ g/L iron, 0.6 μ g/L iodine, 0.5 μ g/L bromines, 0.07 μ g/L sulphur,
1.4 μ g/L metasilicic acids, 1.1 μ g/L heavy carbonics, 320 μ g/L calcium, 0.6 μ g/L magnesium, 0.001 μ g/L lithiums, 0.14 μ g/L selenium, 0.02 μ
G/L copper, 0.04 μ g/L strontiums.
A kind of Pelteobagrus fulvidraco artificial breeding of embodiment 3 and the method for raising
Present invention is disclosed it is a kind of using warm mineral water, spermatozoa preservative fluid carry out advanced fry of yellow catfish artificial breeding method,
Comprise the following steps：
1) 29 DEG C are maintained pond temperature adjustment and using 83 DEG C or so of warm mineral water.Temperature in use sensor is to water
Temperature is monitored in real time.29 DEG C of temperature will be transferred in the common pond reinforced cultivating 3 Pelteobagrus fulvidraco parent more than age of more than 30 days
In mineral water, female-male proportion 1:1.2, carry out Pelteobagrus fulvidraco parent reinforced cultivating 10 days, and feed high protein feed (ratio of crude protein
Example is 39%) every morning 9：00, afternoon 16：00, once, feeding is to eat up as amount in 1 hour every time for each feeding.
2) the Pelteobagrus fulvidraco parent after reinforced cultivating is subjected to artificial induced spawning, using yellow by human chorionic gonadotropin (HCG), rush
The mixing derivant of body generation hormone-releasing hormone analog 2 (LRH-A2) and DOM (DOM) composition induces Pelteobagrus fulvidraco at 29 DEG C
The maturation of female and male gametophyte.HCG, LRH-A2, DOM per the injection of kg body weight female Pelteobagrus fulvidraco are respectively 1100IU, 26 micrograms, 9
Milligram；Using double injection method, the 1st injection dosage is that total amount is injected for second after 2/7,9 hours；Milter dosage halves, and
Only injection 1 time, synchronously injected in the 2nd injection of raun.
3) artificial insemination：
After second injection of hormone 16 hours, raun start heat, gametocide Pelteobagrus fulvidraco, spermary is taken, is ground in mortar
It is broken that spermary trituration, the ratio for adding 0.8ml spermatozoa preservative fluids according to 1 gram of spermary trituration add spermatozoa preservative fluid, mixed equal
It is even, obtain the Pelteobagrus fulvidraco seminal fluid for fertilization；
The formula of the spermatozoa preservative fluid is：Every liter of spermatozoa preservative fluid contains 6.5g NaCl, 0.6g MgCl2、0.8g
Na2HPO4·7H2O、0.7g KH2PO4、1.6g CaCl2、0.01g SrCO3、0.1g KIO3, 0.5g 1B selenium, surplus
For water, pH 7；
After preparing seminal fluid, artificial insemination is carried out immediately.While mature egg is squeezed into dry white enamel jorum, use
Syringe (not having to syringe needle) is drawn the seminal fluid leaching prepared and is gently mixed on ovum, and with feather.But Pelteobagrus fulvidraco ovum occupies white enamel
During 4/5ths space of jorum, stopping is squeezed into Pelteobagrus fulvidraco ovum and drenched into seminal fluid, and the wherein volume ratio of seminal fluid and ovum is 0.4：1.It
29 DEG C of warm mineral waters that volume is cumulative volume 5% are added afterwards, are mixed, and are stood 5min, ovum is fully combined with sperm.Will complete by
The ovum rinsing of essence operation several times, washes away watery blood.
4) Pelteobagrus fulvidraco egg hatching.According to 3.4 ten thousand ovum/square metre standard, Pelteobagrus fulvidraco embryonated egg is uniformly spread out into cloth and laid
Have in 29 DEG C of warm mineral water hatcheries of fish nest and hatched, and oxygenation is carried out using micro-pore oxygenation mode.
5) initial stage raises after incubating oosperm：Hatchery temperature mineral water water temperature is maintained to use micro-pore oxygenation mode at 29 DEG C
Abundant oxygenation is carried out, from hatching the 3rd day to the 7th day, every morning 9：00, afternoon 16：00, a wheel animalcule is respectively fed, by every tail
Fry needs 1-5 wheel animalcule standard to feed every time.
6) advanced fry of yellow catfish is cultivated, and is specifically included：(1) clean up the pond, wild fishes, spiral shell in killing seed rearing pool etc..(2) train
Educate food organisms.The 5th day after cleaning up the pond, 28~32 kgs/acre of peanut press pulps are added in seed rearing pool, inject new water, agitation
The food organisms such as mud, culture of rotifera, cladocera and Copepods.(3) before Pelteobagrus fulvidraco seedling is transferred to seed rearing pool, 83 DEG C are used
Left and right temperature mineral water is by seed rearing pool temperature adjustment and maintains 29 DEG C.(4) fry is put in a suitable place to breed., will according to 250,000 tails/mu standard
The Pelteobagrus fulvidraco seedling of the 11st day is transferred to the seed rearing pool (water temperature maintains 27-29 DEG C) after sterilization after hatching, daily feeding 2 times
Artemia naupiar larva, each scale of feeding are the 5% of fry body weight.
7) feeding management：Proceed by within the 28th day after hatching later stage feeding management, regular water filling, water level maintain 1.0 meters-
Between 1.5 meters.Regulating water quality and fertile degree.Water transparence is maintained between 20-30 centimetres, and dose is (with Diammonium phosphate (DAP) and urine
Based on element) it is defined by water ammonia nitrogen no more than 1mg/L.Every morning 9：00, afternoon 16：00, each red worm of feeding (midge children
Worm), each scale of feeding is the 5% of fry body weight.When fry specification reaches 5-6cm, you can press summer flower sale.
In above-mentioned temperature mineral water containing 225 μ g/L sodium, 0.5 μ g/L iron, 0.6 μ g/L iodine, 0.5 μ g/L bromines, 0.07 μ g/L sulphur,
1.4 μ g/L metasilicic acids, 1.1 μ g/L heavy carbonics, 320 μ g/L calcium, 0.6 μ g/L magnesium, 0.001 μ g/L lithiums, 0.14 μ g/L selenium, 0.02 μ
G/L copper, 0.04 μ g/L strontiums.
Claims (9)
1. a kind of Pelteobagrus fulvidraco artificial breeding and the method for raising, it is characterised in that comprise the following steps：
1) Pelteobagrus fulvidraco parent is raised with 27-29 DEG C of warm mineral water, raises 7~10 days, then Pelteobagrus fulvidraco parent is entered
Row artificial induced spawning；
2) artificial insemination：After artificial induced spawning 16-20h, male spermaries are taken, grind to obtain spermary trituration, add spermatozoa preservative fluid, are mixed
It is even, Pelteobagrus fulvidraco seminal fluid is obtained, while female adult ovum is taken out；By the leaching of Pelteobagrus fulvidraco seminal fluid on ovum, mix, wherein Pelteobagrus fulvidraco seminal fluid
Volume ratio with ovum is 0.2~0.4：1；And then a small amount of 27-29 DEG C of warm mineral water is added, and mix, stand 3~8min, will
Embryonated egg cleans up；
The formula of the spermatozoa preservative fluid is：Every liter of spermatozoa preservative fluid contains 6.5~7.5g NaCl, 0.4~0.6g MgCl2、
0.8~1.2g Na2HPO4·7H2O, 0.7~0.9g KH2PO4, 1.4~1.6g CaCl2, 0.01~0.02g SrCO3、0.1
~0.2g KIO3, 0.5~1.0g 1B selenium, surplus is water, and pH is 7~7.5；
3) Pelteobagrus fulvidraco embryonated egg is uniformly sprinkling upon in 27-29 DEG C of warm mineral water hatchery for be laid with fish nest and hatched, used
Micro-pore oxygenation mode carries out oxygenation；
4) initial stage raises after incubating oosperm：After incubating oosperm, it is 27-29 DEG C to maintain hatchery temperature mineral water water temperature, is used
Micro-pore oxygenation mode carries out abundant oxygenation；The 3rd~7 day after hatching, 2 wheel animalcules are fed daily, and 1-5 are needed every time by every tail fry
Wheel animalcule standard is fed；
5) advanced fry of yellow catfish is cultivated：Before Pelteobagrus fulvidraco seedling is transferred to seed rearing pool, seed is raised using 83 DEG C or so warm mineral waters
Support pond temperature adjustment and maintain 27-29 DEG C；The advanced fry of yellow catfish of the 9th~11 day after hatching is transferred into seed rearing pool to carry out
Constant temperature is raised, and stocking density is 2.5 × 105~3.5 × 105Tail/mu；2 artemia naupiar larvas of feeding daily, each scale of feeding
For the 4.5~5.5% of fry body weight；
6) feeding management：Carry out later stage feeding management within the 28th~33 day after hatching, water level is maintained 1-1.5m, water transparence
20-30cm, the ammonium of application of phosphoric acid two and urea are maintained at, is defined by water ammonia nitrogen no more than 1mg/L；2 red worms of feeding daily, often
Secondary scale of feeding is the 4.5~5.5% of fry body weight, until fry reaches specification；
Contain 225~275 μ g/L sodium, 0.5~0.7 μ g/L iron, 0.6~0.8 μ g/L iodine, 0.3~0.5 μ in above-mentioned temperature mineral water
G/L bromines, 0.05~0.07 μ g/L sulphur, 1.4~1.6 μ g/L metasilicic acids, 1.1~1.3 μ g/L heavy carbonics, 280~320 μ g/L calcium,
0.6~0.8 μ g/L magnesium, 0.001~0.003 μ g/L lithiums, 0.1~0.14 μ g/L selenium, 0.02~0.06 μ g/L copper, 0.02~0.04
μ g/L strontiums.
2. according to the method for claim 1, it is characterised in that in step 1), Pelteobagrus fulvidraco parent is the age to be 3 more than age,
Female-male proportion is 1:0.8~1.2.
3. according to the method for claim 1, it is characterised in that in step 1), the operation of artificial induced spawning is：Using by fine hair
The mixing derivant of film gonadal hormone HCG, luteinizing hormone-releasing hormone analog LRH-A and DOM DOM compositions is in 27-
The maturation of Pelteobagrus fulvidraco female and male gametophyte is induced in 29 DEG C of warm mineral water, per HCG, LRH- of the injection of kg body weight female Pelteobagrus fulvidraco
A2, DOM are respectively 1100~1300IU, 24~26 μ g, 9~11 μ g；Using double injection method, the 1st injection dosage is total amount
Second of injection after 2/9~2/7,9~11h；Milter dosage halves, and only injects 1 time, is synchronously noted in the 2nd injection of raun
Penetrate.
4. according to the method for claim 1, it is characterised in that in step 2), spermary grinds the matter of liquid and spermatozoa preservative fluid
Amount volume ratio is 1g:0.8~1.2ml.
5. according to the method for claim 1, it is characterised in that in step 3), Pelteobagrus fulvidraco embryonated egg, which is sprinkling upon, is laid with fish nest
Hatchery density for 2.5~3.4 ten thousand ovum/square metre.
6. according to the method for claim 1, it is characterised in that in step 5), seed rearing pool first passes through in advance to be cleaned up the pond and train
Educate food organisms cultivation.
7. according to the method for claim 6, it is characterised in that it is described clean up the pond for kill seed rearing pool in wild fishes,
Spiral shell.
8. according to the method for claim 6, it is characterised in that the concrete operations that the food organisms are cultivated are：Cleaning up the pond
The 4th~6 day afterwards, 28~32 kgs/acre of peanut press pulps are added in seed rearing pool, new water, agitation mud is injected, cultivates bait life
Thing.
9. the method according to claim 6 or 8, it is characterised in that the food organisms are wheel animalcule, cladocera and/or oar
Sufficient class biology.
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