CN105850807A - Artificial propagation method of Phoxinus lagowskii Dybowskii - Google Patents
Artificial propagation method of Phoxinus lagowskii Dybowskii Download PDFInfo
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- 238000000034 method Methods 0.000 title claims abstract description 23
- 241001637825 Rhynchocypris lagowskii Species 0.000 title abstract description 7
- 241000251468 Actinopterygii Species 0.000 claims abstract description 107
- 230000012447 hatching Effects 0.000 claims abstract description 15
- 238000002360 preparation method Methods 0.000 claims abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 70
- 235000013601 eggs Nutrition 0.000 claims description 17
- 102000002322 Egg Proteins Human genes 0.000 claims description 11
- 108010000912 Egg Proteins Proteins 0.000 claims description 11
- 210000004681 ovum Anatomy 0.000 claims description 11
- 239000007924 injection Substances 0.000 claims description 10
- 238000002347 injection Methods 0.000 claims description 10
- 239000003814 drug Substances 0.000 claims description 9
- 230000000384 rearing effect Effects 0.000 claims description 9
- 230000003187 abdominal effect Effects 0.000 claims description 8
- 230000002196 ecbolic effect Effects 0.000 claims description 7
- 238000004519 manufacturing process Methods 0.000 claims description 7
- 206010002091 Anaesthesia Diseases 0.000 claims description 6
- 230000037005 anaesthesia Effects 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 6
- 108700012941 GNRH1 Proteins 0.000 claims description 5
- 239000000579 Gonadotropin-Releasing Hormone Substances 0.000 claims description 5
- 230000037396 body weight Effects 0.000 claims description 5
- 229940079593 drug Drugs 0.000 claims description 5
- 230000000638 stimulation Effects 0.000 claims description 5
- 239000000758 substrate Substances 0.000 claims description 5
- QCDWFXQBSFUVSP-UHFFFAOYSA-N 2-phenoxyethanol Chemical compound OCCOC1=CC=CC=C1 QCDWFXQBSFUVSP-UHFFFAOYSA-N 0.000 claims description 4
- 210000004602 germ cell Anatomy 0.000 claims description 4
- 229960005323 phenoxyethanol Drugs 0.000 claims description 4
- 210000000582 semen Anatomy 0.000 claims description 4
- 230000000694 effects Effects 0.000 claims description 3
- 230000002085 persistent effect Effects 0.000 claims description 3
- 101100224827 Caenorhabditis elegans dom-3 gene Proteins 0.000 claims description 2
- 206010010071 Coma Diseases 0.000 claims description 2
- 238000010276 construction Methods 0.000 claims description 2
- 239000003673 groundwater Substances 0.000 claims description 2
- 230000032696 parturition Effects 0.000 claims description 2
- 239000008188 pellet Substances 0.000 claims description 2
- 238000003825 pressing Methods 0.000 claims description 2
- 230000001737 promoting effect Effects 0.000 claims description 2
- 239000003488 releasing hormone Substances 0.000 claims description 2
- 238000000926 separation method Methods 0.000 claims description 2
- 238000011031 large-scale manufacturing process Methods 0.000 abstract description 7
- 238000009395 breeding Methods 0.000 abstract description 5
- 230000001488 breeding effect Effects 0.000 abstract description 5
- 238000011161 development Methods 0.000 abstract description 2
- 230000018109 developmental process Effects 0.000 abstract description 2
- 238000004088 simulation Methods 0.000 abstract description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 6
- 239000001301 oxygen Substances 0.000 description 6
- 229910052760 oxygen Inorganic materials 0.000 description 6
- 230000009027 insemination Effects 0.000 description 5
- 241000252206 Cypriniformes Species 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 238000007726 management method Methods 0.000 description 2
- 210000003097 mucus Anatomy 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 239000004575 stone Substances 0.000 description 2
- 239000013589 supplement Substances 0.000 description 2
- 241000252210 Cyprinidae Species 0.000 description 1
- 241000252233 Cyprinus carpio Species 0.000 description 1
- 241000145619 Phoxinus Species 0.000 description 1
- 230000003444 anaesthetic effect Effects 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
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- 239000000796 flavoring agent Substances 0.000 description 1
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- 239000007789 gas Substances 0.000 description 1
- 210000002149 gonad Anatomy 0.000 description 1
- 230000026109 gonad development Effects 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
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- 229930014626 natural product Natural products 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
- 235000021401 pellet diet Nutrition 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses an artificial propagation method of Phoxinus lagowskii Dybowskii and belongs to the technical field of artificial propagation of fishes. The method comprises six steps including preparation of an artificial spawning ground, selection of parent fishes, breeding of the parent fishes, artificial spawning inducement, natural spawning and natural hatching. Through strict control of various links, particularly simulation of natural spawning ground environment conditions, the artificial spawning ground is prepared, so that the Phoxinus lagowskii Dybowskii can spawn, be fertilized and be hatched smoothly in the artificial spawning ground under the condition of artificial spawning inducement, and the phenomenon that the parent fishes do not spawn due to arrangement of artificial fish nests during artificial spawning inducement in the past is eliminated. The spawning inducement rate, the fertilizing rate and the hatching rate of the Phoxinus lagowskii Dybowskii are effectively increased, the spawning inducement rate is 95% or higher, the fertilizing rate is up to 86.8%, the hatching rate of fertilized ova is up to 84.6%, large-scale production of Phoxinus lagowskii Dybowskii fries is realized, requirements of breeding enterprises and fishermen for large-scale fry breeding are met, and the method has the important significance in development of large-scale breeding of the Phoxinus lagowskii Dybowskii.
Description
Technical field
The invention belongs to artificial propagation of fish technical field, especially relate to a kind of colder water small fishes and draw
The artificial fecundation method of family name.
Background technology
Laplace (Phoxinus lagowskii Dybowskii) is under the jurisdiction of Cypriniformes on taxonomy of fishes
Cypriniformes, Cyprinidae Cyprinidae, Leuciscinae Leuciscinae, genus Phoxinus
Agassiz, fisherman is referred to as Radix Salicis Babylonicae.Laplace is the small-sized economic fish that rivers are wild, its fine and tender taste,
In meat flavour deliciousness, muscle, protein content is (17.88 ± 0.63) %, and fat content is (3.95 ± 0.05) %,
Nutritious, it is the small-sized economic fish of high-quality that people like, has become, at northern area, the cultivation that pond is new
Kind, market prospect is the most considerable.This fish inhabits that water temperature is on the low side, the river of water quality clarification under field conditions (factors)
In stream, the waters such as tributary being distributed mainly on Heilungkiang, TUMENJIANG RIVER, the Yalu River, the Liaohe River, Upper-middle Reaches of The Yellow River.
This fish is inclined cold water fishes, water temperature 0 DEG C～26 DEG C of living, the most suitable growth water temperature 15 DEG C～24 DEG C, 2 rheological properties
Maturation, is the Fish that in batches lay eggs, and lays eggs 2 times～3 times at northern area natural conditions next year, spawning season
5～July, ripe general 10g～30g of individual weight.Laplace is individual maximum one in genus, manually
Cultivation is maximum individual up to more than 500g.
Fishing for due to environmental pollution and people's transition in recent years, the population quantity of this fish gradually decreases, and causes
Market supply wretched insufficiency.North fisherman takes to purchase wild fry and carries out pond culture to meet the market demand,
But due to wild seed limited amount, cannot meet the demand in market, the most relevant Laplace is artificial at all
The research of proliferation, domestic rarely seen Zhang Yongquan [University of Anhui's journal (natural science edition), 2015,39 (4),
92-96.], Luo little Nian [aquatic science, 2013,32 (11), 673-675.], Zu Xiujie [aquaculture,
2015, (6): 9-12.] etc. in terms of artificial propagation the fragmentary report of test, Zhang Yongquan uses artificial insemination
Method obtains insemination ovum, and owing to Laplace individuality is little, epidermal mucus is many, artificial insemination operations's difficulty, it is impossible to
Carrying out seed large-scale production, the method that Luo little Nian uses small size pond sandstone substrate pond to hang Petiolus Trachycarpi fish nest makes
Its natural spawning, gas stone oxygen supplement bottom the method employing, non-water flow stimulates, and parent fish gonad development is bad, produces
After ovum, parent fish cannot remove pond of laying eggs, and parent fish eats ovum phenomenon seriously puerperal, and germ cell greatly can quilt
Parent fish eats up, and emergence rate can be substantially reduced.Zu Xiujie uses and lays Petiolus Trachycarpi fish nest and stone bottom round spawning pond
The method of head makes its natural spawning, the method small scale, it is impossible to large-scale mass production.Therefore prior art
In the middle of need badly want a kind of novel technical scheme to solve this problem.
Summary of the invention
The technical problem to be solved is: provide the artificial fecundation method of a kind of Laplace fish, simulation
Laplace natural spawning site manufacture of intraocular spawning ground, artificial induced spawning, the method for natural spawning, it is achieved that seed
Large-scale production, occurs not concentrating, laying eggs in batches and show during solving existing wild Laplace natural spawning
There are artificial induced spawning, artificial insemination that artificial propagation uses, it is impossible to the problem carrying out large-scale production, meet
Large-scale cultivation produces the needs of seed.
The artificial fecundation method of a kind of Laplace fish, is characterized in that: comprise the following steps,
Step one, the preparation of artificial spawning field
Manufacture of intraocular spawning ground, spawning ground is the width suitable for reading prismatoid water more than end opening width of a length of 30m
Mud sump, and water inlet side a width of 8.0m suitable for reading, end opening a width of 6.0, horizontal plane suitable for reading is the deepest away from pond dolly
Degree is for 50cm, and sidewall gradient ratio is for 1:2；Outlet side wide 8.0m, end opening width 4.0m suitable for reading, suitable for reading
Horizontal plane is 100cm away from vertical depth at the bottom of pond, and sidewall gradient compares 1:2；Described prismatoid include end wall I,
At the bottom of sidewall I, end wall II, sidewall II and pond, described end wall I, sidewall I, end wall II and sidewall II
Upper edge in same level；The upper edge of described end wall I is 50cm far from the vertical dimension at the bottom of pond, on end wall I
End is provided with water inlet, and the upper edge of described end wall II is 100cm far from the vertical dimension at the bottom of pond, and end wall II lower end sets
It is equipped with outlet, and the position of water inlet is in the horizontal direction higher than the position of outlet；At the bottom of described pond and
Sidewall I and sidewall II be upwards provided with gravel layer at 9cm～11cm along at the bottom of pond；
By water inlet water filling in spawning ground, it is 30cm deeply to end wall I side pool inner water, end wall II side
Pool inner water stops water filling for 80cm deeply；
Step 2, selection parent fish source
Choose and derive from the pond culture marketable fish of more than 2 years individuality, and female individuals is at more than 40g, male
Property more than individuality 30g；
Step 3, parent fish are cultivated
It is to cultivate in the pond at the bottom of sandstone that the parent fish chosen in described step 2 is placed on substrate, commodity of throwing something and feeding
Grain feedstuff, uses 24 cun of immersible pumps at Yuan Chi diagonal angle recirculated water, and the flow of every pump is 60m3/ h, often
It circulation 2 times, circulation time is respectively 3:00～6:00 in morning and 18:00～21:00 in afternoon；Carry out
Parent fish rearing, to water temperature stability more than 16 DEG C, continues one week, selects abdominal part to expand, and ovum grain size is uniform
And have the female parent fish of separation and the light male parent fish pressing abdominal part to have milky seminal fluid to flow out standby；
Step 4, artificial induced spawning
Taking the parent fish that described step 3 obtains, it is in 1.5% phenoxyethanol anesthesia liquid that parent fish is put into concentration, makes
Parent fish is anaesthetized, and anaesthetizes and goes into a coma to fish body, and with injecting oxytocic drug after towel fish body, oxytocic is for promoting Huang
Voxel releasing hormone LRH-A3With DOM DOM, liquid measure is 0.2ml, and female parent fish dose of medicament is every
Kg body weight injection luteinizing hormone releasing hormone LRH-A310 μ g/kg～12 μ g/kg and DOM DOM 3
Mg/kg～4mg/kg, male parent fish dosage is that per kilogram of body weight injects luteinizing hormone releasing hormone LRH-A3
5 μ g/kg～6 μ g/kg and DOM DOM 1.5mg/kg～2mg/kg；Carry out the female parent fish hastened parturition and
The proportioning of male parent fish is 1:1.2；
Step 5, lay eggs
Female parent fish and male parent fish after described step 4 proportioning of learning from else's experience throw in construction in described step one
In artificial spawning field, carrying out stimulation by running water, discharge is 0.4m3/ h～0.5m3/ h, water temperature is 16 DEG C～17 DEG C,
Stimulation by running water, Effect time is 36h～40h, and the persistent period of laying eggs is 5h～8h；After Gunther parent fish spawning the 2nd day
At water outlet, valve is opened, with net cage, parent fish is picked out, be transplanted on outdoor parent fish rearing pond；Parent fish moves
After going out, water inlet side, spawning ground water level being added to 30cm, outlet side water level adds to 80cm；
Step 6, hatching
Germ cell is hatching naturally in artificial spawning field, under conditions of water temperature 16 DEG C～17 DEG C, carries out flowing water
Hatching, discharge controls at 0.3m3/ h, the seedling-growing time of incubating oosperm is 102h34min～106h52min.
In the commercial pellet feedstuff thrown something and fed in described step 3, protein content is 32%～35%, and fat content is
3.5%～4.0%.
By above-mentioned design, the present invention can bring following beneficial effect: a kind of Laplace fish artificial
Propagation method, simulates Laplace natural spawning site manufacture of intraocular spawning ground, artificial induced spawning, the side of natural spawning
Method, it is achieved that seed large-scale production, occur during solving existing wild Laplace natural spawning not concentrating,
Lay eggs and the artificial induced spawning of existing artificial propagation employing, artificial insemination, it is impossible to carry out large-scale production in batches
Problem, meets large-scale cultivation and produces the needs of seed.
Accompanying drawing explanation
Below in conjunction with the drawings and specific embodiments, the present invention is further illustrated:
Fig. 1 is the structural representation of the artificial fecundation method artificial spawning field of the present invention a kind of Laplace fish.
Fig. 2 is the FB(flow block) schematic diagram of the artificial fecundation method of the present invention a kind of Laplace fish.
In figure at the bottom of 1-end wall I, 2-sidewall I, 3-end wall II, 4-sidewall II, 5-pond, 6-water inlet, 7-
Outlet.
Detailed description of the invention
The artificial fecundation method of a kind of Laplace fish, as in figure 2 it is shown, mainly comprise the steps that
Step one, the preparation of artificial spawning field: Laplace artificial induced spawning, the success or failure of natural spawning breeding, close
Key is the environmental condition in spawning ground.Under natural environmental condition, Laplace spawning ground on the bank, river course having current,
At depth of water 30-50cm, water quality is pure and fresh, substrate is gravel.Key technical problems of the present invention is for simulating natural product
Ovum environment manufacturing artificial spawning ground, meets Laplace and lays eggs the requirement of physiological habit.As it is shown in figure 1, lay eggs
Field is the width suitable for reading prismatoid cement pit more than end opening width of a length of 30m, and water inlet 6 side is suitable for reading
A width of 8.0m, end opening a width of 6.0, horizontal plane suitable for reading is 50cm away from vertical depth at the bottom of pond, and sidewall gradient ratio
For 1:2；Outlet 7 side wide 8.0m suitable for reading, end opening width 4.0m, horizontal plane suitable for reading is away from vertical depth at the bottom of pond
For 100cm, sidewall gradient compares 1:2；Described prismatoid includes end wall I 1, sidewall I 2, end wall II 3, side
At the bottom of wall II 4 and pond 5, the upper edge of described end wall I 1, sidewall I 2, end wall II 3 and sidewall II 4 with
In one horizontal plane；The upper edge of described end wall I 1 is 50cm far from the vertical dimension of 5 at the bottom of pond, and end wall I 1 upper end sets
Being equipped with water inlet 6, the upper edge of described end wall II 3 is 100cm far from the vertical dimension of 5 at the bottom of pond, bottom end wall II 3
It is provided with outlet 7, and the position of water inlet 6 is in the horizontal direction higher than the position of outlet 7；Described pond
The end 5 and sidewall I 2 and sidewall II 4 at the bottom of pond 5 upwards 9cm～11cm, be provided with gravel layer；Enter
Higher than at the bottom of pond, outlet 7 side at the bottom of pond, side, the mouth of a river 6, slope ratio is for 1:60, and pond, water inlet 6 side is the deepest
50cm, pond, outlet 7 side vertically deep 100cm, when laying eggs, water level adds to water inlet 6 side depth of water 30cm,
Outlet 7 side depth of water 80cm；Before the water filling of artificial spawning field, one layer of (10cm of cloth at the side slope of at the bottom of pond and both sides
Left and right) gravel that differs in size, gravel is the decoy laid eggs of Laplace and the attachment of germ cell.
Step 2, parent fish source: the parent fish that lays eggs derives from the pond culture marketable fish of more than 2 years, female individual
More than body 40g, male more than 30g, anosis without wound.Sex judging to be carried out and female in election process
The collocation of male ratio.Raun is without gonapophysis, and abdominal part is relatively big, and milter has gonapophysis, and light pressure abdominal part has milky
Seminal fluid flows out.Male and female proportioning 1:1.2 is advisable.
Step 3, parent fish rearing: be placed in parent fish rearing pond by the parent fish chosen and cultivate, parent fish is trained
Educating pond and require that water quality is pure and fresh, substrate is sandstone.Throw something and feed during parent fish rearing protein content 32%～35%,
The artificial hard pellet diet of fat content 3.5%～4.0%, feeding volume grasps flexibly according to fish situation of ingesting, and one
As less than parent fish body weight 5%.Parent fish rearing to promote gonad Rapid development with stimulation by running water.
Step 4, artificial induced spawning: when water temperature stability is more than 16 DEG C, check the mature condition of parent fish, uses
Dig egg apparatus to be dug out by the ovum of female parent fish, when the color of ovum becomes cinerous from xanthochromia, and when ovum grain separates, ovum
Grow to IV end of term, carried out artificial induced spawning so that it is oestrus and lay eggs.
First have to carry out the discriminating of male and female parent fish during artificial induced spawning, female parent fish without gonapophysis, abdominal part expands,
Softness, male parent fish has gonapophysis, and light pressure abdominal part has milky seminal fluid to flow out.
Owing to Laplace is little shape Fish, selected parent fish individual weight is generally between 30g～100g, scale
Producing parent fish quantity used more, this fish body surface smooth, mucus is many, operates extremely difficult during injection, with fiber crops
After parent fish is anaesthetized by liquor-saturated medicine, injection operation is fairly simple, and time-consuming.Anaesthetic is phenoxyethanol,
It is in 1.5% phenoxyethanol anesthesia liquid that parent fish is put into concentration, carries out oxytocic drug injection again after making parent fish anesthesia,
During anesthesia, dosage must be grasped, and dosage is excessive easily causes parent fish death, dosage too small parent fish recovery time
Too short, this dosage is under the conditions of water temperature 16 DEG C～17 DEG C, and parent fish revives for 12 minutes～15 minutes, if note
Penetrate parent fish quantity too much, can take to anaesthetize the method in batches injected in batches.After raun injection, carry out male parent
The anesthesia injection of fish.Male and female parent fish proportioning 1:1.2.
Oxytocic drug uses luteinizing hormone releasing hormone (LRH-A3) and DOM (DOM), dosage is dam
Fish injection LRH-A310 μ g/kg～12 μ g/kg and DOM 3mg/kg～4mg/kg, male parent fish dosage halves.
Liquid medicine injection is 0.2ml, and injection site is dorsal fin base portion.Use a shot.
Step 5, natural spawning: parent fish is directly put into artificial spawning after having injected oxytocic drug by male and female parent fish
In Chang, and with stimulation by running water, the inlet valve control of the flow of water, flow-control is at 0.4m3/ h～0.5m3/ h,
During water temperature 16 DEG C～17 DEG C, Effect time is generally 36h～40h, persistent period 5h～8h of laying eggs.Parent fish has produced
Ovum second day, opens spawning ground bottom valve, and the water within spawning ground is released slowly, water outlet net
Parent fish is picked out by case, is put in outdoor parent fish rearing pond.After parent fish removal, outlet is closed, is filled to
Outlet side water level 80cm.
Step 6, naturally hatching: hatching can use water purification to hatch naturally, it is possible to uses Lotic hatching, water purification
Hatching often to measure Spawning water dissolved oxygen, keeps dissolved oxygen at more than 5mg/L, and dissolved oxygen reduces employing micropore
Oxygen increasing equipment oxygen supplement.During Lotic hatching, flow-control is at 0.3m3/h.Water temperature is at 16 DEG C～17 DEG C of incubating oosperms
Seedling-growing time is 102h34min～106h52min.Hatching carries out the fry later stage by chinese carp management method after emerging
Management.
The present invention takes to simulate Laplace natural spawning site manufacture of intraocular spawning ground, makes spawning ground and natural environment
Intending mutually, spawning ground scale is big, meets Laplace reproduction enviromental conditions for spawning, makes Laplace human controllable's
Under the conditions of carry out large-scale production.
Claims (2)
1. an artificial fecundation method for Laplace fish, is characterized in that: comprise the following steps,
Step one, the preparation of artificial spawning field
Manufacture of intraocular spawning ground, spawning ground is the width suitable for reading prismatoid water more than end opening width of a length of 30m
Mud sump, and water inlet (6) side a width of 8.0m suitable for reading, end opening a width of 6.0, horizontal plane suitable for reading is away from pond dolly
The straight degree of depth is 50cm, and sidewall gradient ratio is for 1:2；Outlet (7) side wide 8.0m suitable for reading, end opening width
4.0m, horizontal plane suitable for reading is 100cm away from vertical depth at the bottom of pond, and sidewall gradient compares 1:2；Described prismatoid bag
Include at the bottom of end wall I (1), sidewall I (2), end wall II (3), sidewall II (4) and pond (5), described end
The upper edge of wall I (1), sidewall I (2), end wall II (3) and sidewall II (4) is in same level；
The upper edge of described end wall I (1) is 50cm far from the vertical dimension of (5) at the bottom of pond, and end wall I (1) upper end is arranged
Having water inlet (6), the upper edge of described end wall II (3) is 100cm far from the vertical dimension of (5) at the bottom of pond, end wall
II (3) lower end is provided with outlet (7), and the position of water inlet (6) is in the horizontal direction higher than water outlet
The position of mouth (7)；(5) at the bottom of described pond and sidewall I (2) and sidewall II (4) along (5) at the bottom of pond
Upwards it is provided with gravel layer at 9cm～11cm；
By water inlet (6) water filling in spawning ground, it is 30cm deeply to end wall I (1) side pool inner water,
End wall II (3) side pool inner water stops water filling for 80cm deeply；
Step 2, selection parent fish source
Choose and derive from the pond culture marketable fish of more than 2 years individuality, and female individuals is at more than 40g, male
Property more than individuality 30g；
Step 3, parent fish are cultivated
It is to cultivate in the pond at the bottom of sandstone that the parent fish chosen in described step 2 is placed on substrate, commodity of throwing something and feeding
Grain feedstuff, uses 24 cun of immersible pumps at Yuan Chi diagonal angle recirculated water, and the flow of every pump is 60m3/ h, often
It circulation 2 times, circulation time is respectively 3:00～6:00 in morning and 18:00～21:00 in afternoon；Carry out
Parent fish rearing, to water temperature stability more than 16 DEG C, continues one week, selects abdominal part to expand, and ovum grain size is uniform
And have the female parent fish of separation and the light male parent fish pressing abdominal part to have milky seminal fluid to flow out standby；
Step 4, artificial induced spawning
Taking the parent fish that described step 3 obtains, it is in 1.5% phenoxyethanol anesthesia liquid that parent fish is put into concentration, makes
Parent fish is anaesthetized, and anaesthetizes and goes into a coma to fish body, and with injecting oxytocic drug after towel fish body, oxytocic is for promoting Huang
Voxel releasing hormone LRH-A3With DOM DOM, liquid measure is 0.2ml, and female parent fish dose of medicament is every
Kg body weight injection luteinizing hormone releasing hormone LRH-A310 μ g/kg～12 μ g/kg and DOM DOM 3
Mg/kg～4mg/kg, male parent fish dosage is that per kilogram of body weight injects luteinizing hormone releasing hormone LRH-A3
5 μ g/kg～6 μ g/kg and DOM DOM 1.5mg/kg～2mg/kg；Carry out the female parent fish hastened parturition and
The proportioning of male parent fish is 1:1.2；
Step 5, lay eggs
Female parent fish and male parent fish after described step 4 proportioning of learning from else's experience throw in construction in described step one
In artificial spawning field, carrying out stimulation by running water, discharge is 0.4m3/ h～0.5m3/ h, water temperature is 16 DEG C～17 DEG C,
Effect time is 36h～40h, and the persistent period of laying eggs is 5h～8h；After Gunther parent fish spawning the 2nd day in outlet (7)
Valve is opened by place, is picked out by parent fish with net cage, is transplanted on outdoor parent fish rearing pond；After parent fish removal, will
Spawning ground water inlet (6) side water level adds to 30cm, and outlet (7) side water level adds to 80cm；
Step 6, hatching
Germ cell is hatching naturally in artificial spawning field, under conditions of water temperature 16 DEG C～17 DEG C, carries out flowing water
Hatching, discharge controls at 0.3m3/ h, the seedling-growing time of incubating oosperm is 102h34min～106h52min.
The artificial fecundation method of a kind of Laplace fish the most according to claim 1, is characterized in that: described
In the commercial pellet feedstuff thrown something and fed in step 3, protein content is 32%～35%, fat content be 3.5%～
4.0%.
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