CN102524128A - Artificial reproduction method for Pseudobagras ussuriensis - Google Patents
Artificial reproduction method for Pseudobagras ussuriensis Download PDFInfo
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention discloses an artificial reproduction method for Pseudobagras ussuriensis and aims to solve the problem in the prior art that the maturity of male adult Pseudobagras ussuriensis gonads is poor under routine parent fish breeding and induced spawning conditions and the artificial reproduction is implemented by killing fish and collecting sperms. The method allows natural maturity, natural spermiation and estrus of male Pseudobagras ussuriensis by adding exogenous hormones and changing environmental conditions. Besides, the sperms resulting from natural spermiation have a high degree of maturity, which provides guarantee for fertilization rate. Both natural fertilization and artificial fertilization can successfully produce a large amount of fertilized eggs. More importantly, male and female parent fish are not injured and both applied to artificial reproduction production in the next year, which provides germplasm support for seed preservation and breeding of Pseudobagras ussuriensis. The invention overcomes the problem in the prior art that the artificial reproduction is implemented only by killing fish and collecting sperms.
Description
Technical field
The present invention relates to a method of artificial breeding of fish consumption, in particular to a ussuri intends Chang artificial breeding methods.
Background technology
Ussuri intends Chang (Pseudobagrus? Ussuriensis) belonging Siluriformes (Silurformes), BAGRID (Bagridae), is intended to Chang (Pseudobagrus), located in China's Heilongjiang River and the Pearl River to the Yangtze River and Huaihe River of Taihu Lake, Hongze Lake and other waters, but in recent years its resources plummeted.This oppresses thorn between matter delicacy, delicious flavour, no flesh, the market demand is vigorous; And feeding habits are wide, thermophilic strong (about heatproof 0-36 ℃), disease is few, growth rate is very fast, and extremely consumers in general and raiser like, it is very wide to culture future.Therefore, carrying out its artificial propagation and marketable fish cultures very necessary.
The artificial propagation of fish technology be with female, male parent fish rearing to gonad maturity, promote parent population to oestrus, lay eggs through the artificial induced spawning measure again or arrange smart acquisition fertilized egg, build the adapt circumstance condition then, make incubating oosperm go out the technology of fry.Wherein, obtaining fertilized egg in enormous quantities is the key factor that artificial propagation of fish is produced.
The method that fertilized egg is obtained in the artificial propagation of fish both at home and abroad has two kinds, and the one, through the method acquisition fertilized egg of male and female parent population natural spawning, fertilization in spawning pond; The 2nd, adopt artificial insemination method to obtain fertilized egg.Our existing ussuri intends Chang artificial breeding methods are used: the same pool created by conventional methods male and female broodstock.When hastening parturition, dam fish double injection ocyodinic, 30%, the 2 injection surplus of injecting total ID for the 1st time; A milter shot is carried out when raun the 2nd pin is injected, and dosage is the half the of raun ID.Under this technical scheme condition because the milter spermary can't full maturity, can not arrange naturally smart with oestrus, therefore can't be through the method acquisition fertilized egg of female, male parent population natural spawning, fertilization in spawning pond; Also the artificial insemination method that does not injure male parent population can't be adopted, after the effect time after hastening parturition arrives,,, seminal fluid can't be extruded because milter spermary maturity is poor although the dam fish can be extruded mature egg smoothly.Therefore, produce at present to adopt and kill the artificial insemination method acquisition fertilized egg that fish is got essence, promptly kill milter earlier; Take out spermary; With the spermary dilution of milling, after the most of sperm of microscopy has vigor, artificial again mature egg is extruded into to contain carry out smart ovum in the ovum basin and mix and obtain fertilized egg.Since many years Ussuri intends Chang fish breeding, the male fish could be used for many years as a parent retained as captive breeding or germplasm group reservations.Therefore, the existing technology is used to kill the male fish sperm artificial propagation method is devastated germplasm resources, sustainable Chang is not consistent with the direction of development.
Summary of the invention
The lack of the prior art, the present invention solves the technical problem is to provide a captive breeding ussuri intended Chang method, this method can not damage the case of male broodstock through natural spawning, fertilization or artificial insemination method to obtain law large quantities of high-quality eggs and hatched fry, not only can improve the fertilization rate, more important is to preserve the male parent groups for next year propagation.
To solve the above problems, the present invention is achieved technical solutions: one intended Chang Ussuri artificial breeding methods, especially, comprising the following steps:
(1) selection of parent population
Select complete, undamaged 3 ages of fin ray and above parent population;
(2) cultivation of parent population
Female, male parent population is put into different cultivation ponds respectively cultivate, from artificial induced spawning beginning in previous month, in male parent fish rearing pond, throwing something and feeding contains the artifical compound feed of testosterone hormone;
(3) artificial induced spawning
Female, the male parent population injection oxytocic drug of selective maturation; Female, male parent population is all taked biphasic injection; Dam fish dosage is: inject luteotropin releasing hormone d-ala analog No. 2 for the first time; For the second time inject luteotropin releasing hormone d-ala analog No. 2, human chorionic gonadtropin and DOM, the dosage of milter is 70% of raun, the time interval of double injection is 14～20 hours;
(4) promote that parent population oestruses
Adopting miniflow water qi of chong channel ascending adversely or being interrupted flushing method stimulates the parent population after hastening parturition, and the condition that controls environment;
(5) fertilization
Obtain fertilized egg through natural spawning, fertilization or test-tube method;
(6) artificial incubation
Fertilized egg is put into the hatchery hatch, controlled water flow, water quality, water temperature and dissolved oxygen keep good incubation condition.
Preferably, in the Ussuri intended Chang artificial propagation method, the step (2), the hormone testosterone is 17α-methyl testosterone.
Preferably, in the Ussuri intended Chang artificial propagation method, the artificial testosterone-containing compound feed preparation method: warm water with a small amount of flour in the mix in the synthesis of thin paste, then dilute paste testosterone and stir, and then the thin paste with adequate mixing with artificial feed pellets, placed in a cool dry ventilated place.
Preferably, in the Ussuri intended Chang artificial propagation method, the testosterone hormone pellets with artificial mass ratio of 20mg: 1kg.
Preferably, in the Ussuri intended Chang artificial propagation method, the artificial protein content of feed particles is not less than 20%.
Preferably, in the Ussuri intended Chang artificial propagation method, the step (3), the female broodstock in the first injection of luteinizing hormone-releasing hormone analogues on the 2nd dose of 5μg ~ 8μg/kg weight, female broodstock in the second injection luteinizing hormone-releasing hormone analogues on the 2nd dose of 10μg ~ 15μg/kg body weight dose of hCG 500IU ~ 700IU/kg weight, Domperidone at a dose of 2mg ~ 4mg/kg weight.
Preferably, in the Ussuri intended Chang artificial propagation method, the step (4) in the post-spawning male and female broodstock according to the ratio of 1:1 to 5:6 into the spawning pool for natural spawning, fertilization.
Preferably, in the Ussuri intended Chang artificial propagation method, the step (5), the artificial insemination method: Extrusion three female broodstock egg liquid, while a male's semen extrusion or broodstock sucked out with a straw, and then the egg fluid and semen mixed implementation of artificial insemination.
Compared with the prior art, the present invention intends Chang according ussuri male gonadal development of adult fish in the late Chang held their sex hormone levels decline unique variation, through artificial feeding of exogenous hormones and other technologies intended to make the Ussuri Chang male good natural maturation, ejaculation and natural estrus.And, arrange the sperm that smart mode obtains naturally, its spermioteleosis degree is high, and fertilization rate is secure.Adopt natural insemination or test-tube method all can successfully obtain fertilized egg in enormous quantities.More importantly, the male and female broodstock will not be damaged, the male and female broodstock Following Chang can be used for next year's production of artificial breeding, the male fish to carry out conservation and breeding work laid the germplasm protection .Avoid prior art to adopt and killed the mode of production that fish is got smart method completion artificial propagation.
Description of drawings
In order to be illustrated more clearly in the embodiment of the invention or technical scheme of the prior art; To do to introduce simply to the accompanying drawing of required use in embodiment or the description of the Prior Art below; Obviously, the accompanying drawing in describing below only is some embodiments of the present invention, for those of ordinary skills; Under the prerequisite of not paying creative work, can also obtain other accompanying drawing according to these accompanying drawings.
Figure 1 shows the hair to be Chang Ming Wusu artificial breeding methods in a block diagram.
Embodiment
In order further to understand the present invention, below in conjunction with embodiment the preferred embodiment of the invention is described, describe just to further specifying feature and advantage of the present invention but should be appreciated that these, rather than to the restriction of claim of the present invention.
Participation in Figure 1, specific embodiments of the present invention discloses a method intended Chang ussuri artificial propagation method comprises the following steps:
(1) selection of parent population
Select complete, undamaged 3 ages of fin ray and above parent population;
(2) cultivation of parent population
Female, male parent population is put into different cultivation ponds respectively cultivate, from artificial induced spawning beginning in previous month, in male parent fish rearing pond, throwing something and feeding contains the artifical compound feed of testosterone hormone;
(3) artificial induced spawning
Female, the male parent population injection oxytocic drug of selective maturation; Female, male parent population is all taked biphasic injection; Dam fish dosage is: inject No. 2, luteotropin releasing hormone d-ala analog (LRH-A2) for the first time; For the second time inject luteotropin releasing hormone d-ala analog No. 2 (LRH-A2), human chorionic gonadtropin (HCG) and DOMs (DOM), the dosage of milter is 70% of raun, and the time interval of double injection is 14～20 hours;
(4) promote that parent population oestruses
Adopting miniflow water qi of chong channel ascending adversely or being interrupted flushing method stimulates the parent population after hastening parturition, and the condition that controls environment;
(5) fertilization
Obtain fertilized egg through natural spawning, fertilization or test-tube method;
(6) artificial incubation
Fertilized egg is put into the hatchery hatch, controlled water flow, water quality, water temperature and dissolved oxygen keep good incubation condition.
Research shows; Male fish is in testis development is oestrused etc. process with row's essence; Need a large amount of estradiol (E2) and testosterone (T) hormone; And T is the precursor of synthetic E2, and (17 α-MT) possibly directly or through its metabolite act on spermary promote the milter testis development ripe to add external source 17 Alpha-Methyl testosterones.The study also found that the inventor, T levels in male fish ussuri intended Chang testicular development and ejaculation estrus and other physiological processes play a crucial regulatory role.
After throwing something and feeding and add the feed of 17 α-MT in the embodiment of the invention month before hastening parturition, T content can be kept higher level in the male parent population body, and spermary maturation acceleration, Spermatogenic action are remarkable; Simultaneously, when hastening parturition, female, male parent population is all taked 2 injections; The dosage of hastening parturition of male parent population per weight is 70% of raun, and builds the method for control environment condition, can further promote arranging essence and oestrus naturally of milter; Thereby; Can obtain high quality fertilized egg of scophthatmus in enormous quantities through natural spawning, fertilization or test-tube method, and hatch fry not damaging on the basis of female, male parent population.
In the step (1), select individual big, physique is healthy and strong, anosis, fin ray is complete, undamaged 3 ages and above parent population, is preferably annual February (under the natural environment temperature) option date of parent population.Under the artificial controlled condition of environmental temperature, also can not receive the constraint on date.
In the step (2), female, male parent population is put into different cultivation ponds respectively and cultivates, and the stocking rate of parent population is preferably 667m
2Put in a suitable place to breed about 100kg, and raise together with flower silver carp 100 tails～200 tails that specification is 15cm～20cm.
The parent fish rearing pond be preferably placed at the water source nearer, annotate that row is convenient, the quiet place of environment.Smooth at the bottom of the pond, mud is less, area 660m
2～1300m
2, depth of water 1.3m～1.8m, advancing discharge outlet has barricade.The preparation of ponds meets the conventional fry and fingerling technical specification (SC/T1008-94) of cultivating in pond.
The testosterone hormone is preferably 17 Alpha-Methyl testosterones (17 α-MT).
The preparation method who contains the artifical compound feed of testosterone hormone is: become thin pulp to stick with paste with a small amount of flour mix in warm boiling water; Again 17 Alpha-Methyl testosterones (white powder, odorless, tasteless) are evenly admixed during thin pulp sticks with paste; Then this thin pulp is stuck with paste and the abundant mix of artificial compound feed; Acquisition contains the artifical compound feed of testosterone hormone, after being placed on the shady and cool ventilation place at last and drying up, can throw something and feed.
Protein content must be thrown something and fed greater than 20% artificial compound feed in the parent fish rearing pond.Setting up bait table at parent fish pond, throws something and feeds on bait table in the location.At 9 o'clock in the morning threw something and fed 30% and 70% respectively with at 17 o'clock in afternoon.Each daily ration, feeding quantity has been ingested with 60min and has been advisable, the active situation that the bait of throwing something and feeding will note observing fish, and the bait of throwing something and feeding is not superfluous.Daily ration of feeding is referring to table 1.Water temperature reaches more than 17 ℃ (hastening parturition preceding 1 month) and rises; In male parent fish pond, throw something and feed and contain 17 Alpha-Methyl testosterones ((the quality proportioning of testosterone hormone and artificial compound feed is 20mg: the artificial compound feed of hormone 1kg), one stops to throw something and feed before hastening parturition for 17 α-MT).Throw something and feed in the female parent fish pond and do not add the artifical compound feed of hormone.
Table 1 ussuri intended Chang broodstock amount of feeding
| Temperature | Feeding volume (accounting for the % of parent population body weight) |
| 10℃～15℃ | 1.5 |
| 15℃～20℃ | 2～3 |
| 20℃～30℃ | 3～4 |
Female, Xiong Qinyupeiyuchi requires: water quality is pure and fresh, and dissolved oxygen is more than 5mg/L, and pH 7～8, are strictly on guard against the parent population floating head.In May, every separated 10d～15d changes Chi Shui once.
In the step (3), the optimum condition of artificial induced spawning:
A) time: be preferably mid or late May to early June.
B) water temperature: be stabilized in more than 22 ℃.
C) spawning inducing pool: adopt the cement pit of miniflow water, area is 10m
2～50m
2, depth of water 0.6m～1.0m.
D) artificial solar protection devices: solar protection devices will be covered in the top, hatchery of hastening parturition, and available bamboo stake, steel pipe etc. are fixing, loam cake black puggaree.
E) selection of mature brood fish: the raun belly expands softness, and gonopore is little infrared prominent, presses belly that sense of movement is arranged with have gentle hands.Male parent population seminal fluid can not be extruded, and gonapophysis is little red and expand.
F) injection of ocyodinic:
(1) injecting method: the injection of pectoral fin base portion, concrete grammar is: syringe needle towards the fish cephalad direction, is become 45 inserting needle 2mm～3mm gently, inject body cavity fast.
(2) preparation of ocyodinic and consumption: mix to use ocyodinic also to divide the effect of hastening parturition of biphasic injection better: the dosage of dam fish luteotropin releasing hormone d-ala analog No. 2 (LRH-A2) when injecting for the first time is 5 μ g～8 μ g/kg body weight, and the dosage of dam fish luteotropin releasing hormone d-ala analog No. 2 (LRH-A2) when injecting for the second time is that the dosage of 10 μ g～15 μ g/kg body weight, human chorionic gonadtropin (HCG) is that the dosage of 500IU～700IU/kg body weight, DOM (DOM) is 2mg～4mg/kg body weight.The milter consumption is 70% of a raun.The equal biphasic injection of female, milter, needle gage are 14～20 hours.
Step (4), the effect of Ussuri intends Chang estrus time and temperature, the type of drug oxytocin, broodstock maturity, micro-water and climate conditions have a direct relationship between the effects of time, the most important factor affecting water temperature (see Table 2 ).
Table 2 Effect of time Ussuri intended Chang
| Water temperature | 19℃～22℃ | 23℃～25℃ | 26℃～28℃ | 29℃～30℃ |
| The effect time | 24～22h | 21～19h | 18～16h | 14～11h |
Environmental condition when promoting the sexual maturity is preferably: keep quite, lucifuge, dissolved oxygen amount are sufficient, water temperature 24-28 ℃.Preceding 4h～5h the bath of oestrusing stimulates oestruses, make milter can be well row's essence naturally, and with raun synchronization of estrus.
In the step (5), the method for fertilization comprises two kinds:
(1) natural spawning, fertilization: natural spawning, fertilization female: male proportioning is 1: 1～5: 6; Female, male parent population after being about to hasten parturition is put into spawning pond in 1: 1～5: 6 ratio; Realize natural spawning, fertilization, the concrete realization as follows: after having injected the second pin ocyodinic, female, male parent population is put into spawning pond in 1: 1～5: 6 ratio; Artificial fish nest is set in the spawning pond; The fish nest is with making such as willow coring, brown sheet or polythene net sheets, and a part hangs on water body, at the bottom of another part tiling pond.Adopt the hydrostatic qi of chong channel ascending adversely or be interrupted bath, the preceding 4h that oestruses～5h bath stimulates oestruses (time of oestrusing calculated through the effect time in the table 1), and will wash by water when oestrusing to stimulate changes fine bourn into.Before and after the effect time, milter chases raun, and along the mad trip of pool side, afterbody and dorsal fin surface frequently, forms the spray; Be sunken on the water-bed fish nest under raun begins to rotate and lay eggs, milter also chases raun to forward on the fish nest of bottom and arranges essence, and this macrura reevesii nest has adhered to a large amount of fertilized eggs, can the fish nest be moved into miniflow water hatching in the hatchery.
(2) artificial insemination: test-tube hero: female proportioning is 1: 3, promptly extrudes the ovum liquid of 3 tail dam fishes, simultaneously the suction pipe sucking-off is extruded or used to the seminal fluid of the male parent population of 1 tail, then ovum liquid and seminal fluid is mixed, and realizes artificial insemination.The concrete realization as follows: after having injected the second pin ocyodinic; Female, male parent population is put into spawning pond in 3: 1 ratio, artificial fish nest is set in the spawning pond, the fish nest is with making such as willow coring, brown sheet or polythene net sheets; A part hangs on water body, at the bottom of another part tiling pond.Adopt the hydrostatic qi of chong channel ascending adversely or be interrupted bath, the preceding 4h that oestruses～5h bath stimulates oestruses.Want activity situation and the part fish of the close observation fish situation of laying eggs before and after the effect time; Chase raun, surface, form indivedual parent populations such as spray phenomenon of oestrusing frequently along the mad trip of pool side, afterbody and dorsal fin and occur if any milter; Or check when the fish nest finds to have a small amount of self-produced fertilized egg to adhere to; All should fish for the parent population inspection immediately, when press female parent population belly to have ovum liquid to flow out smoothly with have gentle hands after, can carry out manual work and squeeze ovum and insemination from gonopore.Employing semi-dry process insemination after the body surface of soon female, male parent population and the moisture of vessel are dried with dry towel, is extruded 3 tail raun ovum liquid earlier; Simultaneously the suction pipe sucking-off is extruded or used to the seminal fluid of milter; Pour into fast in the ovum liquid behind the physiological saline dilution seminal fluid with about 5mL 0.5% immediately, with goose feather it was mixed about 10 seconds gently, the physiological saline that adds 15～20mL0.5% more fully mixed smart ovum more than 20 seconds; Ovum is fertilized fully; Then it is sprinkled on the fish nest in the water of hatchery equably, fertilized egg promptly sticks on the fish nest after about 20 seconds, can get into the miniflow water hatching stage.
In the step (6), preferably, the fish nest that has adhered to a large amount of fertilized eggs is placed on carries out flowing water hatching in the miniflow water cement pond.Will keep 24 hours continuous flowing water during this time, water quality is good, contamination-free, and water temperature is at 23 ℃～28 ℃, and dissolved oxygen is more than 6mg/L.Fertilized egg needs 50h～70h can hatch prelarva (fry) approximately.
In order to further specify technical scheme of the present invention; Below in conjunction with embodiment the preferred embodiment of the invention is described; Describe just to further specifying feature and advantage of the present invention but should be appreciated that these, rather than to the restriction of claim of the present invention.
Embodiment one:
The inventor has done experiment for the first time in February, 2010 to June according to technique scheme, and its practical implementation method is following:
(1) selection of parent population
February, select individual large, infirmity, disease, fin intact without damage 3 years old and above Ussuri intended Chang broodstock, where male broodstock 660 (average per tail 215.3g).
(2) cultivation of parent population
Female, male parent population is put into different cultivation ponds respectively to be cultivated; Begin from April 22 (the pond water temperature reaches 17 ℃); In the cultivation pond of male parent population, add and contain the 17 Alpha-Methyl testosterones (artifical compound feed of 17 α-MT); Throw something and feed continuously 30～36 days (artificial propagation was thrown something and fed 30 days for 1,2 batches continuously, and artificial propagation was thrown something and fed table 3 36 days for 3,4 batches continuously).(artifical compound feed of 17 α-MT) is admixed in the artificial compound feed as adhesive with a small amount of flour and is processed, and (17 α-MT) the quality proportioning with artificial compound feed is 20mg: 1kg to 17 Alpha-Methyl testosterones to contain 17 Alpha-Methyl testosterones.
(3) artificial induced spawning and fertilization, hatching
The parent population of selective maturation: the raun belly expands softness, and gonopore is little infrared prominent, presses belly that sense of movement is arranged with have gentle hands.Male parent population seminal fluid can not be extruded, and gonapophysis is little red and expand.
May 23 onwards technical solution according to the invention Artificial propagation of four groups (Table 3), which, on May 23 and May 28 two batches of fish breeding artificial insemination method, according to the male and female broodstock 3: The proportion of a spawning broodstock 98 ussuri intended Chang group (ie, 33 male and 98 female broodstock broodstock) and 137 groups (ie, 46 male and 137 female broodstock broodstock), male and female broodstock technology in accordance with the present invention Methods 2 oxytocin, female broodstock dose: the first injection of luteinizing hormone-releasing hormone analogues on the 2nd (LRH-A2) 8μg/kg weight, the second injection of luteinizing hormone-releasing hormone analogues on the 2nd (LRH- A2) 10μg/kg body weight, human chorionic gonadotropin (HCG) 500IU/kg weight and Domperidone (DOM) 4mg/kg weight, male a dose of 70% of females, two injections at intervals of 20 hours.Spawning rate is respectively 82.4% and 86.3%; Fertilization rate reached 86.6% and 83.1%; Hatch fry 16.2 ten thousand tails and 18.6 ten thousand tails; Incubation rate reaches 72.1% and 69.5%.Female, male parent population is not damage all.
June 2 and June 6 two batches of fish, according to the male and female ratio of 5:6, respectively, broodstock spawning broodstock 156 Ussuri intended Chang group (ie, 188 male and 156 female broodstock broodstock) and 260 groups (ie 312 male and 260 female broodstock broodstock), female broodstock doses: the first injection of luteinizing hormone-releasing hormone analogues on the 2nd (LRH-A2) 5μg/kg weight, the second injection of luteinizing hormone-releasing hormone analogues No. 2 (LRH-A2) 15μg/kg body weight, human chorionic gonadotropin (HCG) 700IU/kg weight and Domperidone (DOM) 2mg/kg body weight dose males 70% females, two injections The time interval is 14 hours.Adopt natural spawning, fertilization method breeding, spawning rate is respectively 85.6% and 88.4%; Fertilization rate reached 89.5% and 86.6%; Hatch fry 23.2 ten thousand tails and 36.4 ten thousand tails; Incubation rate reaches 64.2% and 67.6%.Female, the male parent population in postpartum is not damage all.
Table 32010 years ussuri intended Chang Statistics of artificial propagation
Embodiment two:
The inventor has done experiment for the second time in February, 2011 to June according to technique scheme, and its practical implementation method is following:
(1) selection of parent population
February, select individual large, infirmity, disease, fin intact without damage 3 years old and above Ussuri intended Chang broodstock, where male broodstock 854 (average per tail 206.5g).
(2) cultivation of parent population
Female, male parent population is put into different cultivation ponds respectively to be cultivated; Begin from April 22 (the pond water temperature reaches 17 ℃); In the cultivation pond of male parent population, add and contain the 17 Alpha-Methyl testosterones (artifical compound feed of 17 α-MT); Throwing something and feeding continuously, (artificial propagation was thrown something and fed 28 days for 1,2 batches continuously, and artificial propagation was thrown something and fed 32 days for 3,4 batches continuously, and artificial propagation was thrown something and fed 38 days for 5,6,7 batches continuously in 28～38 days.Table 4).(artifical compound feed of 17 α-MT) is admixed in the artificial compound feed as adhesive with a small amount of flour and is processed, and (17 α-MT) the quality proportioning with artificial compound feed is 20mg: 1kg to 17 Alpha-Methyl testosterones to contain 17 Alpha-Methyl testosterones.
(3) artificial induced spawning and fertilization, hatching
The parent population of selective maturation: the raun belly expands softness, and gonopore is little infrared prominent, presses belly that sense of movement is arranged with have gentle hands.Male parent population seminal fluid can not be extruded, and gonapophysis is little red and expand.
May 20 - June 15, oxytocin Ussuri intends Chang female broodstock 1096, male broodstock 761, male and female broodstock are taking second injection, which
Adopt spermatozoa insemination method's (male and female ratio=1: 3) carry out artificial propagation production of technical scheme of the present invention 20～June 2 May; Hasten parturition respectively female, male parent population 557 tails and 187 tails; Dam fish dosage is: inject No. 2 (LRH-A2) 8 μ g/kg body weight of luteotropin releasing hormone d-ala analog for the first time; Inject for the second time No. 2 (LRH-A2) 15 μ g/kg body weight of luteotropin releasing hormone d-ala analog, human chorionic gonadtropin (HCG) 700IU/kg body weight and DOM (DOM) 2mg/kg body weight; The dosage of milter is 70% of raun, and the time interval of double injection is 20 hours.
Adopt 6～June 15 June the natural spawning, fertilization method (male and female than=10: 11 or 10: 10) of technical scheme of the present invention to carry out artificial propagation production; Hasten parturition respectively female, male parent population 539 tails and 574 tails; Dam fish dosage is: inject No. 2 (LRH-A2) 5 μ g/kg body weight of luteotropin releasing hormone d-ala analog for the first time; Inject for the second time No. 2 (LRH-A2) 10 μ g/kg body weight of luteotropin releasing hormone d-ala analog, human chorionic gonadtropin (HCG) 500IU/kg body weight and DOM (DOM) 4mg/kg body weight; The dosage of milter is 70% of raun, and the time interval of double injection is 14 hours.
Reproductive effect is seen table 4.The mean spawning rate 86.2%, average fertilization rate 86.3%, average incubation rate 71.5% obtains the fry amount altogether and reaches 168.4 ten thousand tails.Female, the male parent population in postpartum is not damage all.
Table 42 011 years ussuri intended Chang Statistics of artificial propagation
In summary, the present invention has the advantage: According ussuri intends Chang male gonadal development of adult fish in the late sex hormone levels continue to drop its unique variation, through artificial feeding of exogenous hormones and environmental conditions such as technology, enabling Ussuri Nature intended Chang male nicely mature ejaculation and natural estrus.And, arrange the sperm that smart mode obtains naturally, its spermioteleosis degree is high, and fertilization rate is secure.Adopt natural insemination or test-tube method all can successfully obtain fertilized egg in enormous quantities.The more important thing is that female, male parent population all can not sustain damage, female, male parent population all can continue on for the artificial propagation production of next year, for guarantor's kind and the breeding work of carrying out these male fish have been established the germplasm guarantee.Avoid prior art to adopt and killed the mode of production that fish is got smart method completion artificial propagation.
The explanation of above embodiment just is used for helping to understand method of the present invention and core concept thereof.Should be pointed out that for those skilled in the art, under the prerequisite that does not break away from the principle of the invention, can also carry out some improvement and modification to the present invention, these improvement and modification also fall in the protection domain of claim of the present invention.
To the above-mentioned explanation of the disclosed embodiments, make this area professional and technical personnel can realize or use the present invention.Multiple modification to these embodiment will be conspicuous concerning those skilled in the art, and defined General Principle can realize under the situation that does not break away from the spirit or scope of the present invention in other embodiments among this paper.Therefore, the present invention will can not be restricted to these embodiment shown in this paper, but will meet and principle disclosed herein and features of novelty the wideest corresponding to scope.
Claims (8)
1 A ussuri intends Chang artificial breeding methods, characterized in that it comprises the following steps:
(1) selection of parent population
Select complete, undamaged 3 ages of fin ray and above parent population;
(2) cultivation of parent population
Female, male parent population is put into different cultivation ponds respectively cultivate, from artificial induced spawning beginning in previous month, in male parent fish rearing pond, throwing something and feeding contains the artifical compound feed of testosterone hormone;
(3) artificial induced spawning
Female, the male parent population injection oxytocic drug of selective maturation; Female, male parent population is all taked biphasic injection; Dam fish dosage is: inject luteotropin releasing hormone d-ala analog No. 2 for the first time; For the second time inject luteotropin releasing hormone d-ala analog No. 2, human chorionic gonadtropin and DOM, the dosage of milter is 70% of raun, the time interval of double injection is 14～20 hours;
(4) promote that parent population oestruses
Adopting miniflow water qi of chong channel ascending adversely or being interrupted flushing method stimulates the parent population after hastening parturition, and the condition that controls environment;
(5) fertilization
Obtain fertilized egg through natural spawning, fertilization or test-tube method;
(6) artificial incubation
Fertilized egg is put into the hatchery hatch, controlled water flow, water quality, water temperature and dissolved oxygen keep good incubation condition.
(2) according to claim 1, wherein the Ussuri intends Chang artificial breeding methods, wherein: said step (2), the hormone testosterone is 17α-methyl testosterone.
3 according to claim 1, wherein the Ussuri intends Chang artificial breeding methods, wherein: said artificial testosterone-containing compound feed preparation method: warm water with a small amount of flour in the mix in the synthesis of thin paste, then Testosterone dilute paste and stir, and then the thin paste with adequate mixing with artificial feed pellets, placed in a cool dry ventilated place.
4 according to claim 3 ussuri intends Chang artificial breeding methods, wherein: said testosterone pellets with artificial mass ratio of 20mg: 1kg.
5 according to claim 3 ussuri intends Chang artificial breeding methods, wherein: said artificial pellet the protein content of not less than 20%.
6 according to claim 1, wherein the Ussuri intends Chang artificial breeding methods, wherein: said step (3), the female broodstock in the first injection of luteinizing hormone-releasing hormone analogues on the 2nd dose of 5μg ~ 8μg/kg weight, female broodstock in the second injection luteinizing hormone-releasing hormone analogues on the 2nd dose of 10μg ~ 15μg/kg body weight dose of hCG 500IU ~ 700IU/kg weight, to Europe one dose of 2mg ~ 4mg/kg body weight.
As claimed in claim 1, wherein said artificial breeding ussuri Chang proposed method characterized in that: said step (4), the oxytocin after male and female broodstock 1:1 ~ 5:6 ratio into production eggs in the pool for natural spawning, fertilization.
8 according to claim 1, wherein the Ussuri intends Chang artificial breeding methods, wherein: said step (5), the artificial insemination method: Extrusion three female broodstock egg liquid, while a male broodstock semen extrusion or sucked out with a straw, and then the egg fluid and semen mixed implementation of artificial insemination.
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| CN103404461A (en) * | 2013-08-16 | 2013-11-27 | 淮阴师范学院 | Artificially induced gynogenesis method for Pseudobagrus ussuriensis |
| CN103404461B (en) * | 2013-08-16 | 2015-08-05 | 淮阴师范学院 | Ussuriensis Artificial gynogenesis method |
| CN107980672A (en) * | 2017-12-10 | 2018-05-04 | 云南省渔业科学研究院 | A kind of method of artificial induction's fat Bangladesh dace breeding |
| CN107980672B (en) * | 2017-12-10 | 2021-04-27 | 云南省渔业科学研究院 | Method for artificially inducing propagation of dace labrocarpa |
| CN109349171A (en) * | 2018-11-30 | 2019-02-19 | 河南省水产科学研究院 | A kind of quasi- Chang Ecology propagation method |
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| CN110250052B (en) * | 2019-07-04 | 2022-04-26 | 淮阴师范学院 | Breeding method for pseudobagrus ussuriensis hypoxia-resistant family |
| CN110250054A (en) * | 2019-08-06 | 2019-09-20 | 安徽省农业科学院水产研究所 | A kind of parent culture method promoting Pelteobagrus fulvidraco gonad development |
| CN114793959A (en) * | 2022-05-06 | 2022-07-29 | 昆明市水产科学研究所 | Artificial whitebait propagation method for improving survival rate of postpartum parent fish |
| CN114793959B (en) * | 2022-05-06 | 2023-03-14 | 昆明市水产科学研究所 | Whitebait artificial propagation method for improving survival rate of postpartum parent fish |
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