CN114916474A - Artificial breeding method for Pseudobagrus Zhongguchi - Google Patents

Artificial breeding method for Pseudobagrus Zhongguchi Download PDF

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Publication number
CN114916474A
CN114916474A CN202210569150.9A CN202210569150A CN114916474A CN 114916474 A CN114916474 A CN 114916474A CN 202210569150 A CN202210569150 A CN 202210569150A CN 114916474 A CN114916474 A CN 114916474A
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fish
pseudobagrus
breeding method
artificial
water
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张丽媛
杨剑虹
陶毅波
王慧
严辉
许国明
黄茂
熊清海
蒋荣明
李晶
董学文
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Anning Friendship Ornamental Fish Breeding Co Ltd
Kunming Fisheries Research Institute
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Anning Friendship Ornamental Fish Breeding Co Ltd
Kunming Fisheries Research Institute
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00—Culture of aquatic animals
    • A01K61/10—Culture of aquatic animals of fish
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K61/00—Culture of aquatic animals
    • A01K61/10—Culture of aquatic animals of fish
    • A01K61/17—Hatching, e.g. incubators
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/20—Animal feeding-stuffs from material of animal origin
    • A23K10/22—Animal feeding-stuffs from material of animal origin from fish
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/20—Animal feeding-stuffs from material of animal origin
    • A23K10/26—Animal feeding-stuffs from material of animal origin from waste material, e.g. feathers, bones or skin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/158—Fatty acids; Fats; Products containing oils or fats
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/174—Vitamins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87—Re-use of by-products of food processing for fodder production

Abstract

The invention provides an artificial breeding method of pseudobagrus ussuriensis, and belongs to the technical field of aquaculture. The artificial breeding method of the Pseudobagrus Zhongbuttockii comprises the steps of carrying out reinforced cultivation on parent fishes of the Pseudobagrus Zhongbuttockii in 1 month of each year to obtain mature female fishes and mature male fishes; pairing the mature female fish and the mature male fish in an environment of a spawning medium to obtain fertilized eggs attached to the spawning medium; the fertilized eggs attached to the spawning medium are hatched to obtain the pseudobagrus Zhongguchi fries, and the method simulates the natural environment suitable for the survival of the pseudobagrus Zhongguchi fries through three steps of parent fish intensive cultivation, reproduction and fertilized egg hatching, so that a large amount of pseudobagrus Zhongguchi fries are successfully bred, and the fertilized egg hatching rate is over 75 percent.

Description

Artificial breeding method for Pseudobagrus Zhongguchi
Technical Field
The invention belongs to the technical field of aquaculture, and particularly relates to an artificial breeding method for pseudobagrus ussuriensis.
Background
Pseudobagrus Zhongbuttocknsis (Pseudobagus medinalis Regan) belongs to the order of Viridae, the genus of Pseudobagrus, the local name wire bending, the special species of Yunnan, and is only distributed in the basin of Dian Pond. The main characteristics are as follows: round abdomen, flat caudal side, smooth body surface without scales, fat fin base slightly shorter than hip fin base, 4 pairs of mouth width approximately equal to head width, lower position, no bifurcation of caudal fin, slightly concave middle; the requirement on water quality is high; the food is taken at night and in the dark at night; the group is favored; cave dwelling) is originally common fishes in the Yunnan pond, has few internal organs, more edible parts and tender meat and delicious taste, and is deeply loved by consumers. In this year, the quantity of pseudobagrus ussuriensis gradually decreases, the lake body of the Yunnan pond is completely eradicated, the pseudobagrus ussuriensis is only scattered on a few ponds and caves around the Yunnan pond and enters the lake stream, and the downstream of the Yunnan pond is a part of the river reach of a common river, so that the distribution range of wild species is narrow, the quantity is extremely small, the pseudobagrus ussuriensis is endangered, and the protection needs to be strengthened urgently. The artificial domestication, breeding and seedling raising of the Pseudobagrus mid-buttock is a direct, important and effective means for rescue protection and even subsequent development and utilization. The artificial domestication and breeding of Pseudobagrus Zhongbuttock is not reported at home and abroad at present.
Disclosure of Invention
In view of the above, the present invention aims to provide an artificial breeding method for pseudobagrus ussuriensis, which can obtain a large amount of pseudobagrus ussuriensis.
The invention provides an artificial breeding method for Pseudobagrus mesopterus, which comprises the following steps:
1) in 1 month middle ten days each year, carrying out reinforced cultivation on pseudobagrus ussuriensis parent fish to obtain mature female fish and mature male fish;
2) pairing the mature female fish and the mature male fish in the step 1) in an environment of a spawning medium to obtain fertilized eggs attached to the spawning medium;
3) hatching the fertilized eggs attached to the egg laying medium in the step 2) to obtain pseudobagrus ussuriensis fries.
Preferably, during the intensive culture of the pseudobagrus middle-hip fish in the step 1), the depth of a water body is 65-75 cm, and the temperature of the water body is 18-22 ℃; the temperature difference between day and night is less than or equal to 4 ℃; the dissolved oxygen of the water body is not less than 6 mg/L.
Preferably, the feed in the step 1) comprises the following components in percentage by mass: 80% of animal natural bait and 20% of artificial feed.
Preferably, the artificial feed comprises the following components in percentage by mass: 35% of fish meal, 35% of bean cake, 11.7% of bran, 16% of wheat middling, 1% of meat and bone meal, 1% of fish oil, 0.1% of vitamin for the voronoi fish, 0.1% of voronoi cleaner and 0.1% of voronoi composite probiotics.
Preferably, the feeding method of the feed comprises the steps of feeding twice a day, wherein one time is morning and one time is evening;
the feeding amount of the feed is 3% of the weight of the parent fish.
Preferably, during the intensive breeding and pairing of the pseudobagrus middle gluteus parent fish in the step 1), flushing is further included.
Preferably, the water is flushed every other day for 2 hours every 4 months from late ten days to before sexual maturity of the middle hip pseudobagrus;
the water is flushed for 2 hours each time in the morning and afternoon of 6 months.
Preferably, the oviposition medium in step 2) comprises one or more of the following: palm pieces, nylon mesh and artificial turf.
Preferably, the hatching method in the step 3) is to hatch the fry for 86 hours out of the membrane in a water body with the water temperature of 19-22 ℃ or hatch the fry for 70 hours out of the membrane at the water temperature of 22-24 ℃.
The artificial breeding method for the pseudobagrus mid-buttock provided by the invention is characterized in that in 1 month middle of the year, the pseudobagrus mid-buttock parent fish is subjected to reinforced cultivation to obtain mature female fish and mature male fish; pairing the mature female fish and the mature male fish in an environment of a spawning medium to obtain fertilized eggs attached to the spawning medium; and hatching the fertilized eggs attached to the oviposition medium to obtain pseudobagrus ussuriensis fries. According to the method, through three steps of intensive cultivation, propagation and fertilized egg hatching, a natural environment suitable for the survival of the pseudobagrus ussuriensis is simulated, a large number of pseudobagrus ussuriensis fries are successfully bred, and the fertilized egg hatching rate is over 75%.
The invention further defines a method for strengthening culture, which reduces the energy consumption of the pseudobagrus ussuriensis parent fish as much as possible by strictly defining the water body environment parameters, and simultaneously limits the feed composition and the feeding times to supplement the energy required by the development of sperm eggs, thereby enabling the pseudobagrus ussuriensis parent fish to grow rapidly and achieve sexual maturity.
The invention also specifically defines flushing during the intensive culture. The water flushing method comprises two stages of water flushing, wherein water is flushed in the early stage to stimulate gonad development, and water is flushed in the later stage to stimulate spawning.
Drawings
Fig. 1 is a schematic structure diagram of an incubation container for artificial pairing of pseudobagrus natalensis in the invention.
Detailed Description
The invention provides an artificial breeding method for Pseudobagrus mesopterus, which comprises the following steps:
1) in 1 month middle every year, carrying out reinforced cultivation on pseudobagrus ussuriensis parent fish to obtain mature female fish and mature male fish;
2) pairing the mature female fish and the mature male fish in the step 1) in an environment of a spawning medium to obtain fertilized eggs attached to the spawning medium;
3) hatching the fertilized eggs attached to the oviposition medium in the step 2) to obtain pseudobagrus ussuriensis fries.
In 1 month middle every year, the method carries out reinforced cultivation on pseudobagrus ussuriensis parent fish to obtain mature female fish and mature male fish.
In the invention, because the fish is a temperature-changing animal, the temperature difference between day and night is large, the water temperature is influenced, the water temperature is greatly changed, the body temperature of the fish is in response to the change of the water temperature, more energy is consumed, and the sperm and egg development is influenced. During the intensive cultivation period of the Pseudobagrus middle-buttock parent fish, the depth of the water body is preferably 65-75 cm, and more preferably 70 cm. The depth of the water body is increased relative to the water depth in the non-breeding period, so that the influence of day and night air temperature change on the water temperature can be reduced, and the water temperature is ensured to be basically constant. The temperature of the water body is preferably 18-22 ℃, and more preferably 20 ℃. The temperature difference between day and night is preferably less than or equal to 4 ℃, and more preferably less than or equal to 2 ℃. The dissolved oxygen of the water body is preferably not less than 6mg/L, and more preferably 7-10 mg/L.
In the invention, the feed preferably comprises the following components in percentage by mass: 80% of animal natural bait and 20% of artificial feed. The feed increases the proportion of animal natural bait relative to the feed during non-reproduction periods, and supplements the required amount of protein required for the development of sperms and eggs by increasing the protein intake of pseudobagrus middle gluteus animals. The artificial feed preferably comprises the following components in percentage by mass: 35% of fish meal, 35% of bean cake, 11.7% of bran, 16% of wheat middling, 1% of meat and bone meal, 1% of fish oil, 0.1% of vitamin for the voronoi fish, 0.1% of voronoi cleaner and 0.1% of voronoi composite probiotics. The feeding method of the feed is preferably to feed twice a day, wherein one time is morning (6:00) and one time is night (22: 00). The animal natural bait is preferably frozen red worms. The feeding times are increased in the breeding period, the energy required by the development of sperms and eggs is mainly supplemented, and the rapid and strengthened parent fish cultivation is realized. The feeding amount of the feed is 3% of the weight of the parent fish. In the embodiment of the invention, the head and tail method is to gather the parent fishes and scatter feed.
In the present invention, the pseudobagrus mesopotatus preferably further comprises flushing water during intensive rearing. In late 4 months to before sexual maturity in pseudobagrus ussuriensis, the water is preferably flushed every other day for 2 hours. The flushing method is preferably characterized in that a leather hose is obliquely arranged at the top of the culture pond at an angle of 30 degrees close to the inner side, and the water faucet is controlled. The flushing speed is preferably that the culture pond does not flood water.
In the invention, the Pseudobagrus intermedius age II + reaches sexual maturity, belongs to an egg laying batch type, and male fish individuals with sexual maturity are larger than female fish. The weight of the female fish in the primary sexual maturity is more than 35g, and the total length is more than 130 cm; the weight of the first sexual mature male fish is more than 50g, and the total length is more than 145 cm. The abdomen of the female fish is enlarged, the ovary is obvious, the abdomen is flat and concave when the female fish faces the abdomen, the two sides are raised, and the genital pore is red. The male fish has large reproductive protrusion and is peach-red in congestion.
After obtaining the mature female fish and the mature male fish, the invention carries out 1:1 artificial pairing on the mature female fish and the mature male fish in an environment of a spawning medium to obtain fertilized eggs attached to the spawning medium.
In the invention, the male fish in the breeding period has a nest habit, is very good in fighting, has a hard thorn on the pectoral fin and is easy to fight and hurt, and some male fish even die slowly after being seriously injured, thereby influencing the breeding. Therefore, when the hatching container is matched, the hatching container (shown in figure 1) is divided into a plurality of cells by using the barriers, each cell is internally provided with a nest (tile) and a spawning medium, and each cell only keeps 1 pair of parent fishes (the male-female ratio is 1:1), so that the parent fishes of each pair are not influenced by each other; meanwhile, the small holes are uniformly distributed on the partition plate, so that the water in the hatching container can freely flow in each partition; the tiles can be naturally placed at the bottom of the container in a laying mode, and holes and wires are punched and tied at the two ends of the tiles so that the tiles can be lifted conveniently to observe the spawning condition; and a spawning medium is arranged in each tile. During the pairing period, the parent fish enters the breeding period in 6 months, and the parent fish is flushed with water once every morning and afternoon, 2 hours each time, until the spawning is finished. During pairing, water quality control is well performed, and the water temperature is preferably 18-22 ℃ and more preferably 20 ℃. The dissolved oxygen in the water body is not less than 6mg/L, and more preferably 7-10 mg/L; the photosynthetic bacteria were used to condition the water 1 time every 10 days.
In the invention, a fish nest is preferably arranged in each cell at the bottom of the culture pond by using a cylindrical tile. Laying media are distributed in the fish nests. The oviposition medium preferably comprises one or more of the following: palm pieces, nylon mesh and artificial turf. Experiments show that different materials have different spawning medium effects, and the fertilized egg attachment rate is sequentially palm slice, nylon mesh and artificial turf.
In the invention, compared with a method adopting artificial oxytocin injection, the pairing method is more suitable for fertilization of the Pseudobagrus ussuriensis, and the fertilization rate reaches 91 percent, but the fertilization cannot be realized by the method adopting artificial oxytocin injection, and the analysis reason is probably that the Pseudobagrus ussuriensis in wild breeds has artificial sex hormone stress.
After fertilized eggs attached to the spawning medium are obtained, the method disclosed by the invention can be used for hatching the fertilized eggs attached to the spawning medium to obtain pseudobagrus ussuriensis fries.
In the invention, the egg laying medium with fertilized eggs is transferred to a hatching pond for hatching. The hatching pond is preferably splashed with benzalkonium bromide solution (for aquatic products) in a full pond. The dosage of benzalkonium bromide solution is 0.15g/m 3 1 time every 2 days, 3 times in a row. The hatching method is preferably to hatch the fry for 86 hours to form a membrane in a water body with the water temperature of 19-22 ℃ or hatch the fry for 70 hours to form a membrane at the water temperature of 22-24 ℃, hatch the fry for 86 hours at 19 ℃ and hatch the fry for 70 hours at 24 ℃ under the control of a constant temperature device. By adopting the hatching and cultivating method, the hatching rate is more than 75 percent.
The artificial breeding method for pseudobagrus ussuriensis provided by the invention is described in detail with reference to the following examples, but the examples should not be construed as limiting the scope of the invention.
Example 1
Artificial breeding method for Pseudobagrus Zhongguchi
And (3) strengthening cultivation of the Pseudobagrus middle-buttock parent fish: in 1 middle of the month, the parent fish is intensively cultivated, the water level of the culture pond is increased from 45cm to 70cm in the non-breeding period, and the influence of the temperature difference between day and night on the gonad development is reduced. The bait is changed from 100% artificial feed into feed consisting of 80% animal natural bait (frozen red worm, feeding after thawing) and 20% artificial feed. The feeding time is 22: on the basis of once feeding for 00, once feeding is added at 6:00 in the next day. Beginning in late 4 months, the water is flushed every other day at 8:00 am for 2h each time, and the gonad development is stimulated.
Selecting sexually mature parent fishes: pseudobagrus intermedius age II + reaches sexual maturity, belongs to an egg laying batch type, and male fish individuals with sexual maturity are larger than female fish. The weight of the female fish in the primary sexual maturity is more than 35g, and the total length is more than 130 cm; the weight of the first sexual mature male fish is more than 50g, and the total length is more than 145 cm. The abdomen of the female fish is enlarged, the ovary is obvious, the abdomen is flat and concave when the female fish faces the abdomen, the two sides are raised, and the genital pore is red. The male fish has large reproductive protrusion and is peach-red in congestion.
Flushing and nest distribution in the breeding period: pseudobagrus middle gluteus has a reproductive period of 5-7 months. Selecting sexual mature parent fish, and carrying out artificial intervention propagation. The male fish genital process is obviously convex and reddish; the abdomen of the female fish is touched and is soft; at this point, the male and female 1:1 pair was placed in the fish nest in the pool. From 6 months of the reproductive period, the water is flushed once at 8:00 a.m. every day and once at 14:00 a.m. every afternoon, each time lasts for 2h, the diameter of a flushing pipe is 7cm, the spawning is stimulated, and the water is flushed until the spawning is finished. Observing and recording the ingestion and activity conditions of the parent fish, recording the water temperature change of the culture pond, well managing and controlling the water quality, controlling the water temperature to be 20 +/-2 ℃, regulating the water for 1 time by using photosynthetic bacteria after 10 days, and keeping the good water quality of the culture pond and sufficient dissolved oxygen (more than 6 mg/L).
And (3) arranging a fish nest in each grid at the bottom of the pond by using cylindrical tiles (and ensuring that the number of the fish nests is larger than that of male fishes), placing a palm piece (50cm multiplied by 15cm) which is steamed and torn into a fluffy shape and then disinfected by saline water below each fish nest, and moving the palm piece together with fertilized eggs to an incubation cylinder for incubation. And (5) counting and calculating the attachment rate of the fertilized eggs according to a formula I.
Fertilized egg attachment ratio (%) — number of medium fertilized eggs/total number of fertilized eggs × 100% formula I
The parent fish can lay eggs through free pairing, and the fertilization rate reaches 91 percent.
When hatching fertilized eggs, the benzalkonium bromide solution (for aquatic products) is sprinkled in the whole pool, and the dosage is 0.15g/m 3 1 time every 2 days, 3 times in a row. When the water temperature is 23 ℃, the membrane is hatched for about 70h, and the hatching rate reaches 75 percent.
Comparative example 1
Effect of artificially injected oxytocin on fertilized egg formation
Intensive culture of parent fishes of pseudobagrus ussuriensis was carried out according to the method of example 1, parent fishes with mature gonads were selected and thrown into a culture pond according to the male-female ratio of 1:1, and oxytocin was artificially injected according to the method of table 1.
TABLE 1 conditions of manual oxytocin injection
Figure BDA0003658436000000061
As a result, none of the three groups of oxytocin-injected female fishes were able to lay eggs. The reason for the analysis may be that pseudobagrus ussuriensis in wild breeders has stress to artificial synthetic hormones, and the response mechanism to artificial synthetic hormones remains to be further studied.
Example 2
Influence of different-material spawning media on attachment rate of medium for mid-hip pseudobagrus ussuriensis oosperms
The middle-hip pseudobagrus fish enhancement and pairing were performed as described in example 1, except that the spawning media were set as palm pieces (50cm × 15cm), nylon mesh pieces (48cm × 20cm), and artificial turf (50cm × 20cm), respectively. Wherein the palm pieces are steamed and torn into fluffy shape and then sterilized by saline water. The nylon net and the artificial turf are subjected to solarization treatment after being sterilized by saline water. The fertilized egg attachment condition is observed in the oviposition period. And (5) counting the attachment rate of the fertilized eggs.
The results show that different materials of oviposition media have obvious influence on the attachment rate of fertilized eggs: the fertilized egg attachment rate is sequentially palm piece (95%) > nylon mesh piece (47%) > artificial turf (21%). When buttockss pseudobagrus in artifical breeding, select the palm piece to be favorable to improving the fertilized egg attachment rate as the medium of laying eggs, and then improve and breed efficiency.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and amendments can be made without departing from the principle of the present invention, and these modifications and amendments should also be considered as the protection scope of the present invention.

Claims (9)

1. An artificial breeding method for Pseudobagrus medialis is characterized by comprising the following steps:
1) in 1 month middle ten days each year, carrying out reinforced cultivation on pseudobagrus ussuriensis parent fish to obtain mature female fish and mature male fish;
2) pairing the mature female fish and the mature male fish in the step 1) in an environment of a spawning medium to obtain fertilized eggs attached to the spawning medium;
3) hatching the fertilized eggs attached to the egg laying medium in the step 2) to obtain pseudobagrus ussuriensis fries.
2. The artificial breeding method according to claim 1, wherein during the intensive cultivation of the parent fishes of pseudobagrus ussuriensis in the step 1), the depth of the water body is 65-75 cm, and the temperature of the water body is 18-22 ℃; the temperature difference between day and night is less than or equal to 4 ℃; the dissolved oxygen of the water body is not less than 6 mg/L.
3. The artificial breeding method according to claim 1, wherein the feed in the step 1) comprises the following components in percentage by mass: 80% of animal natural bait and 20% of artificial feed.
4. The artificial breeding method according to claim 3, wherein the artificial feed comprises the following components in percentage by mass: 35% of fish meal, 35% of bean cake, 11.7% of bran, 16% of wheat middling, 1% of meat and bone meal, 1% of fish oil, 0.1% of vitamin for Weinuo fish, 0.1% of Weinuoqin and 0.1% of Weinuo composite probiotics.
5. The artificial breeding method according to claim 1, wherein the feeding method of the feed comprises feeding twice a day, once in the morning and once in the evening;
the feeding amount of the feed is 3% of the weight of the parent fish.
6. The artificial breeding method of claim 1, wherein the step 1) of intensive breeding and pairing of the parent fishes of pseudobagrus ussuriensis further comprises flushing water.
7. The artificial breeding method according to claim 6, wherein the water is flushed every other day for 2 hours each time from late 4 months to before sexual maturity of Pseudobagrus middle-buttock parent fish;
from 6 months, the water is flushed each time for 2h in the morning and afternoon.
8. The artificial breeding method according to claim 1, wherein the oviposition medium in step 2) comprises one or more of the following: palm pieces, nylon mesh and artificial turf.
9. The artificial breeding method according to claim 1, wherein the hatching in the step 3) is hatching the fry for 86 hours and emerging film in a water body with the water temperature of 19-22 ℃ or hatching the fry for 70 hours and emerging film in a water body with the water temperature of 22-24 ℃.
CN202210569150.9A 2022-05-24 2022-05-24 Artificial breeding method for Pseudobagrus Zhongguchi Pending CN114916474A (en)

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