CN104756920B - Huso huso raun and Amur Sturgeon milter hybrid breeding method - Google Patents
Huso huso raun and Amur Sturgeon milter hybrid breeding method Download PDFInfo
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- CN104756920B CN104756920B CN201510188321.3A CN201510188321A CN104756920B CN 104756920 B CN104756920 B CN 104756920B CN 201510188321 A CN201510188321 A CN 201510188321A CN 104756920 B CN104756920 B CN 104756920B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
A kind of Huso huso raun and Amur Sturgeon milter hybrid breeding method, belong to technical field of aquaculture.The method comprising the steps of：First, Juvenile stage：Female parent selects 14+Age Huso huso, male parent select 8+Age Amur Sturgeon；2nd, parent fish rearing and genital regulating：1st, parent fish is preferred；2nd, the feedstuff of parent fish, feeding and fortification；3rd, water temperature genital regulating；4th, breeding condition and daily management；3rd, artificial fertilization and hatching：1st, ovum, semen collection are squeezed；2nd, artificial fertilization；3rd, ovum is washed；4th, incubating oosperm；4th, larva and juvenile and juvenile fish are cultivated.Hybrid sturgeon seed rate of fertilization that this method is cultivated, incubation rate are high, and the characteristics of have the growth traitss such as growth fast, wide adaptability, strong stress resistance excellent, are suitable for the cultivation of China different regions Acipenser Sinensis.
Description
Technical field
The invention belongs to technical field of aquaculture, is related to a kind of Huso huso raun and Amur Sturgeon milter hybrid breeding side
Method.
Background technology
At present, China has become Yang Xun big countries of the world, and about 21000 tons of annual production accounts for world's sturgeon cultivation yield
89%.With the fast development of China's sturgeon cultivation industry, the problems such as hybridize that breed variety is chaotic, there is no excellent Cultivars increasingly
Prominent, seriously constrain the sound development of China's Acipenser Sinensis industry.At present, China's sturgeon cultivation kind mostly is wild and raises and train kind, raw
On product, breed variety is chaotic, mostly is the chaotic cenospecies of some little plants, and culture performance is low.Except China there is no Acipenser Sinensis excellent
Outside kind, the such as country such as the former Soviet Union, Germany, the U.S. and Iran of other sturgeon cultivation big countries cultivates and is suitable for this country's cultivation
The master of environment supports kind.For example, the little huso sturgeon sturgeon (Europe huso sturgeon Husohuso ♀ × sterlet Acipenserruthenus ♂) of the former Soviet Union,
The siberia platform of Germany, height the head sturgeon and paddlefish etc. in the U.S. are respectively provided with the breeding environment in native country and grow fast, premunition
The advantages of strong and early sexual maturity.Therefore, the Acipenser Sinensis new varieties for cultivating suitable China native country cultivation are badly in need of in China, optimize and improve
The structure of China's sturgeon cultivation kind.
Amur sturgeon, sturgeon are Amur Sturgeon and Huso huso, are the special product and important economic fish in Heilungkiang, are only distributed in
Heillongjiang River system.Wherein Amur Sturgeon is the native country sturgeon cultivation species of the earliest independent development of China, because which has individual big, growth
The advantages of fast and meat is good, and being welcome by raiser, accounts for more than the 15% of China's cultured output, but which has and is not easily accepted by
The shortcoming of man-made feeds.Huso huso build is very large, under the conditions of artificial cultivation growth vigor substantially, but its sexual maturity is later than other sturgeons
Fish, about 16-20, these cultivation shortcomings are subject to certain restrictions their cultivation, popularization.Heilungkiang hybrid sturgeon is black
Longjiang Huso huso and the filial generation of Amur Sturgeon, with the presence of a number of individual in the natural water area in Heilungkiang.From 2007
After the Heilungkiang hybrid sturgeon sexual maturity of year artificial cultivation, with other artificial sturgeon breeding variety protections, higher breeding is shown
Performance.And the cultivation experience of many raisers is also indicated that, the growth performance of Heilungkiang hybrid sturgeon is preferable.Therefore, Huso huso raun
There is important grinding with the Amur Sturgeon milter hybrid new breed production of hybrid seeds and seed rearing to the sound development for promoting China's Acipenser Sinensis industry
Study carefully meaning.
Content of the invention
It is an object of the invention to provide a kind of Huso huso raun and Amur Sturgeon milter hybrid breeding method, the method can be cultivated
Acipenser Sinensis new varieties and realize scale seed breeding.High through the hybrid seedlings insemination rate of the method cultivation, incubation rate.
The purpose of the present invention is achieved through the following technical solutions：
A kind of Huso huso raun and Amur Sturgeon milter hybrid breeding method, including：First, Juvenile stage, two, parent fish rearing and
Genital regulating, three, artificial fertilization and hatching, four, larva and juvenile and juvenile fish are cultivated, and are comprised the following steps that：
First, Juvenile stage：Female parent selects 14+Age Huso huso, male parent select 8+Age Amur Sturgeon.
2nd, parent fish rearing and genital regulating：By temperature control and fortification, promote male and female parent fish sexual maturity synchronization, specifically
Require as follows：
1st, parent fish is preferred：Select form, body colour normal, individual big, healthy without the female fish parent fish of 14 Huso husos more than age of wound,
Total length is up to 190～220cm, 60～90kg of body weight；Amur Sturgeon milter up to 8 more than age, 155～175cm of total length, body weight up to 18～
23kg；Preferred parent fish is put in 4 × 25m parent fish rearings pond in batches, male and female quantitative proportion is ♀: ♂=1: 1～1.5；Raise
20～25kg/m of density3；
2nd, parent fish feeding and fortification are cultivated：The feeding of parent fish：Bait is Acipenser Sinensis parent fish expanded pellet diet, feeds day 2 times,
0.5～1wt.% of the day feeding volume for fish body body weight；Parent fish fortification is cultivated：Primiparity sturgeon parent fish and send out through producing sturgeon parent fish gonad
When educating to IV, parent fish fortification cultivation is proceeded by, cultivated before incubating to parent fish product, gonad development is to the V phases；Through producing parent fish, produce
Feedstuff is fed after incubating, and is fed daily 2 times, 1～1.5wt.% of the feeding volume for parent fish body weight, bait：In per kilogram basis bait
Add the chilled fishes of 20wt.% and 3wt.% celluloses in material；
3rd, water temperature genital regulating：When natural temperature is down to 3 DEG C, 3 DEG C of water temperatures 50d are maintained；Then water temperature starts back from 3 DEG C
Rise, daily 1 DEG C of slow rising, until when being increased to 15 ± 0.5 DEG C, hereafter water temperature remains unchanged, routine observation parent fish gonad is sent out
Educate situation；
4th, breeding condition and daily management：Continuous charge, running water technology, the clear pond of timing change water；Condition of water quality：DO≥
6.0mg/L, pH7.0～8.0, hardness 10.0mg/l；Residual bait, blowdown are removed in time.
3rd, artificial fertilization and hatching：Crowded ovum, semen collection → artificial fertilization → wash ovum → incubating oosperm, wherein：Manually receive
Essence, fertilization rate reached to 90%；Hatching water temperature (15 ± 0.5) DEG C, hatches prelarva, incubation rate more than 85% after germ cell 168h.
4th, larva and juvenile and juvenile fish are cultivated：Feedstuff series are water silk earthworm → water silk earthworm+mixed feed → mixed feed.Membrane
1～7 sky and water silk earthworm live bait of prelarva is cultivated 7 after mincing, and cultivates water temperature (18 ± 0.5) DEG C, feeds number of times day for 8 times；Every after 7 days
Secondary when feeding, mixed feed need to be added in 5～7.5wt.% ratios in water silk earthworm and carry out mixing feeding, stop adding after 3 days
Mixed feed, changing live bait and replacing the mode of feeding with mixed bait carries out prelarva acclimatization culture, start to feed daily cooperation number of times 1～
2 times, live bait 6～7 times reduced by a live bait later and feeds number of times per 1～2 day, and the corresponding mixed feed that increases feeds number of times work, protects
Hold feed day sum constant；Through the mixed feed acclimatization culture of prelarva can be completed within 7～10 days.The hybrid sturgeon Seedling that this method is cultivated
Kind of rate of fertilization, incubation rate height (insemination rate reaches more than 90%, and incubation rate reaches more than 85%), and have that growth is fast, adaptability
Extensively, the characteristics of growth traitss such as strong stress resistance are excellent, is suitable for the cultivation of China different regions Acipenser Sinensis.
Specific embodiment
Below technical scheme is further described, but is not limited thereto, every to the technology of the present invention
Scheme is modified or equivalent, without deviating from the spirit and scope of technical solution of the present invention, all should cover in the present invention
Protection domain in.
A kind of Huso huso raun and Amur Sturgeon milter cross-breeding method for culturing seedlings, comprise the following steps：
First, Juvenile stage：
Female parent selects 14 ages above Huso huso raun, male parent to select 8 ages above Amur Sturgeon milter.
2nd, parent fish rearing and genital regulating：
Including：1st, parent fish is preferred；2nd, the feedstuff of parent fish, feeding and fortification；3rd, water temperature genital regulating；4th, bar is cultivated
Part and daily management, particular content are as follows：
1st, parent fish is preferred：Select form, body colour normal, individual big, healthy without the female fish parent fish of 14 Huso husos more than age of wound,
Total length is up to 190～220cm, 60～90kg of body weight；Amur Sturgeon milter up to 8 more than age, 155～175cm of total length, body weight up to 18～
23kg；Preferred parent fish is put in 4 × 25m parent fish rearings pond in batches, male and female quantitative proportion is ♀: ♂=1: 1～1.5；Raise
Density 20kg/m3；
2nd, the feedstuff of parent fish, feeding and fortification are cultivated：The feedstuff of parent fish：Bait is Acipenser Sinensis parent fish expanded pellet diet；Parent
The feeding of fish：Day feeds 2 times, 0.5～1wt.% of the day feeding volume for fish body body weight；Parent fish fortification is cultivated：Automatic control temperature control
Start, add the chilled fishes of 20wt.% and 3wt.% celluloses in per kilogram foundation bait (Acipenser Sinensis commercial feed powder)；Primiparity
Sturgeon parent fish and through produce sturgeon parent fish gonad development to IV when, proceed by parent fish fortification cultivation, cultivate to parent fish produce incubate before, property
Gland is developed to the V phases, feeds daily 2 times, 1～1.5wt.% of the feeding volume for parent fish body weight.
3rd, water temperature genital regulating：Water temperature regulates and controls：When natural temperature is down to 3 DEG C, 3 DEG C of water temperatures 50d are maintained；Then water temperature from
3 DEG C of bottom outs, 1 DEG C of slow rising daily, until when being increased to 15 ± 0.5 DEG C, hereafter water temperature remains unchanged, routine observation parent
Piscinity gland developmental state；
4th, breeding condition and daily management：Daily management：During parent fish rearing, continuous charge, running water technology, the clear pond of timing
Change water；Condition of water quality：DO >=6.0mg/L, pH7.0～8.0, hardness 10.0mg/l；Residual bait, blowdown are removed in time.
3rd, artificial fertilization and hatching：
Including：1st, ovum, semen collection are squeezed；2nd, artificial fertilization；3rd, ovum is washed；4th, incubating oosperm, particular content are as follows：
Cultivate through 50d temperature controls, control light and fortification, the female fish parent fish gonad development of Huso huso is to the IV phases to V phases, outward appearance
The full expansion of raun abdominal part can be observed soft, meanwhile, Amur Sturgeon milter gonad is also ripe；
1st, ovum, semen collection are squeezed：Huso huso parent fish is first fished for, after M-222 anesthesia, ovum is taken with Minimally Invasive Surgery, squeeze ovum inflow and wash one's face
Fertilization is waited in basin；In addition pull Amur Sturgeon milter side squish lip suction pipe collecting semen in advance out and instill another empty beaker；Little per 2
When collection once, 4 DEG C of Refrigerator stores are standby, carry out seminal fluid microscopy before artificial fertilization, select the good seminal fluid of motility of sperm and enter pedestrian
Work is fertilized；
2nd, artificial fertilization：It is fertilized using dry method, adds 500ml cold water, agitation activation essence in the beaker for fill seminal fluid 15ml
Son, pours the sperm that is activated in Sheng ovum container rapidly, quickly stirs 5min, then to Sheng ovum container addition 2000ml water temperatures be
15 ± 0.5 DEG C of pure and fresh fresh water, stirs 1min with feather, and whole fertilization process requires to complete in 10min；
3rd, ovum is washed：After fertilization completes 15min, germ cell is poured into bolting silk and is sewed in the special fishing net for washing ovum, with 15 ±
0.5 DEG C of pure and fresh fresh water washes ovum 3 times；
4th, unsticking：Washed germ cell pour into pre- fill in 5 liter of 20% talcous unsticking cylinder, unsticking 20 minutes；
5th, incubating oosperm：Hatching adopts vial-type couveuse, 30cm × 60cm；Germ cell is placed in each hatching bottle
1.5L, hatching water temperature are 15 ± 0.5 DEG C, and pH7～8, germ cell are hatched through 168h, and prelarva demoulding is hatched.
4th, the cultivation of larva and juvenile and juvenile fish：
Temporarily the foster initial stage, breeding density control is in 3 000～5 000 tails/m2, develop breeding density before sinking to fry and drop to
2 000～3 000 tails/m2；1～7 sky and water silk earthworm live bait of membrane prelarva mince after cultivate 7 days (round glass jar tank 2m ×
0.4m), water temperature (18 ± 0.5) DEG C is cultivated, number of times is fed day for 8 times；When fed after 7 days every time, need in the water silk earthworm by 5～
7.5wt.% ratios interpolation mixed feed (Acipenser Sinensis micropartical open-mouthed bait) carry out mixing and feed, and stop adding after 3 days coordinating and raise
Material, changing live bait and replace the mode of feeding with mixed bait carries out prelarva acclimatization culture, starts to feed daily and coordinates number of times 1～2 time, work
Bait 6～7 times, reduced by a live bait later and feeds number of times per 1～2 day, and the corresponding mixed feed that increases feeds number of times work, is kept for day throw
Feed sum constant.Through the mixed feed acclimatization culture of prelarva can be completed within 7～10 days；Juvenile fish culture pond is having set up the facility that shelters from heat or light
Cement pit or fiberglass pond cultivate based on.Every square metre of water surface of fry breeding density can put the fry 500～1 of 3cm or so in a suitable place to breed
000 tail.
Claims (4)
1. a kind of Huso huso raun and Amur Sturgeon milter hybrid breeding method, it is characterised in that methods described step is as follows：
First, Juvenile stage：Female parent selects 14+Age Huso huso, male parent select 8+Age Amur Sturgeon；
2nd, parent fish rearing and genital regulating：
（1）Parent fish is preferred：Selection form, body colour are normal, individual big, healthy without the female fish parent fish of 14 Huso husos more than age is hindered, entirely
It is up to 190～220cm, 60～90kg of body weight；Amur Sturgeon milter up to 8 more than age, 155～175cm of total length, body weight up to 18～
23kg；Preferred parent fish is put in 4 × 25m parent fish rearings pond in batches, male and female quantitative proportion is ♀: ♂=1：1～1.5；Raise
20～25kg/m of density3；
（2）Parent fish feeding and fortification are cultivated：The feeding of parent fish：Day feed 2 times, day feeding volume for fish body body weight 0.5～
1wt.%；Parent fish fortification is cultivated：Primiparity sturgeon parent fish and through produce sturgeon parent fish, when gonad development is to IV, proceed by parent fish nutrition
Reinforced cultivating, cultivate to parent fish produce incubate before, gonad development to V phase is fed 2 times daily, feeding volume for parent fish body weight 1～
1.5wt.%；
（3）Water temperature genital regulating：When natural temperature is down to 3 DEG C, 3 DEG C of water temperatures 50d are maintained；Then water temperature is from 3 DEG C of bottom outs,
1 DEG C of slow rising daily, until when being increased to 15 ± 0.5 DEG C, hereafter water temperature remains unchanged, routine observation parent fish gonad development feelings
Condition；
（4）Breeding condition and daily management：Continuous charge, running water technology, the clear pond of timing change water；Condition of water quality：DO≥6.0mg/
L, pH7.0～8.0, hardness 10.0mg/l；Residual bait, blowdown are removed in time；
3rd, artificial fertilization and hatching：Crowded ovum, semen collection → artificial fertilization → wash ovum → incubating oosperm；
4th, larva and juvenile and juvenile fish are cultivated：1～7 sky and water silk earthworm live bait of membrane prelarva is cultivated 7 after mincing, cultivation water temperature (18 ±
0.5) DEG C, number of times is fed day for 8 times；When fed after 7 days every time, in water silk earthworm need to add to coordinate in 5～7.5wt.% ratios and raise
Material carries out mixing and feeds, and stops adding mixed feed after 3 days, and changing live bait and replacing the mode of feeding with mixed bait carries out prelarva domestication
Cultivate, it is 1～2 time to feed cooperation number of times during beginning daily, live bait 6～7 times, reduced by live bait later per 1～2 day and feed time
Number, the corresponding mixed feed that increases feed number of times, and it is constant that holding feeds sum day；Raise through the cooperation of prelarva can be completed within 7～10 days
Material acclimatization culture.
2. Huso huso raun according to claim 1 and Amur Sturgeon milter hybrid breeding method, it is characterised in that the step
In rapid two, used in the process of the feeding of parent fish, parent fish bait is Acipenser Sinensis parent fish expanded pellet diet.
3. Huso huso raun according to claim 1 and Amur Sturgeon milter hybrid breeding method, it is characterised in that the step
In rapid two, bait is cultivated in fortification used in parent fish fortification cultivating process：Add in per kilogram parent fish bait
The chilled fishes of 20wt.% and 3wt.% celluloses.
4. Huso huso raun according to claim 1 and Amur Sturgeon milter hybrid breeding method, it is characterised in that the step
In rapid three, the fertilization rate reached of artificial fertilization to 90%；Hatching water temperature (15 ± 0.5) DEG C, hatches prelarva after germ cell 168h, hatch
Rate more than 85%.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107549059A (en) * | 2017-08-15 | 2018-01-09 | 广东省生物资源应用研究所 | A kind of Amur Sturgeon raun and paddlefish milter hybrid breeding method |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105557586A (en) * | 2016-01-13 | 2016-05-11 | 杭州千岛湖鲟龙科技股份有限公司 | Hybrid breeding method for female Siberian sturgeon and male Amur sturgeon |
| CN106069899B (en) * | 2016-06-15 | 2021-12-28 | 中国水产科学研究院长江水产研究所 | Method for realizing natural mating and oviposition of Acipenser dabryanus through artificial regulation |
| CN107018935B (en) * | 2017-05-23 | 2023-07-21 | 中国水产科学研究院黑龙江水产研究所 | Wild device of marijuana fry |
| CN109804951B (en) * | 2019-03-26 | 2022-02-18 | 海南晨海水产有限公司 | Artificial crossbreeding method for large-body mackerel and camel-back bass |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6443097B1 (en) * | 2001-03-16 | 2002-09-03 | University Of Maryland Biotechnology Institute | Recirculating marine aquaculture process |
| ES2194578B1 (en) * | 2001-04-03 | 2005-04-01 | Universidad De Granada | FEED FOR THE FOOD OF THE ESTURION ASCIPENSER NACARII, IN THE PHASES UNDER THE 6 MONTHS UNTIL COMMERCIAL SIZE. |
| CN101697714B (en) * | 2009-11-16 | 2012-07-25 | 中国水产科学研究院黑龙江水产研究所 | Sturgeon incubation method in muddy water body |
| CN103329825A (en) * | 2013-06-17 | 2013-10-02 | 广州市先步农业科技有限公司 | Hybrid sturgeon fingerling domestication and cultivation method |
| CN104365518B (en) * | 2014-11-27 | 2016-09-07 | 四川省农业科学院水产研究所 | Acipenser dabryanus seed opening and acclimation method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107549059A (en) * | 2017-08-15 | 2018-01-09 | 广东省生物资源应用研究所 | A kind of Amur Sturgeon raun and paddlefish milter hybrid breeding method |
| CN107549059B (en) * | 2017-08-15 | 2020-01-07 | 广东省生物资源应用研究所 | Hybrid breeding method for female sturgeon and male paddlefish |
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